CN212140483U

CN212140483U - Sterile experimental animal excrement collecting device

Info

Publication number: CN212140483U
Application number: CN202020433866.2U
Authority: CN
Inventors: 谈善军; 王俊杰; 吴国豪
Original assignee: Zhongshan Hospital Fudan University
Current assignee: Zhongshan Hospital Fudan University
Priority date: 2020-03-30
Filing date: 2020-03-30
Publication date: 2020-12-15
Anticipated expiration: 2030-03-30

Abstract

The utility model relates to an experimental animal excrement sterile collecting device, which comprises a workbench, a fixing belt and a liquid absorbing device; the workbench comprises a drain hole, a connecting pipe column and an absorption device groove; the connecting pipe column is connected with the lower end of the drain hole, and the other end of the connecting pipe column can be detachably connected with an external collecting container; an absorption device groove matched with the liquid absorption device is arranged on the workbench, and the edge area of the absorption device groove is connected with the drainage hole; an absorption window capable of placing absorbent paper is arranged on the absorption device groove; the fixing belt is used for fixing an experimental object to keep the experimental object stable in the experimental process; the connecting pipe column can be detachably connected with a sterile collector with a sterile cap; its advantages are: can separate excrement and urine at the collection in-process, effectively prevent that excrement and urine from being contaminated, and can provide the low temperature experiment environment that special experiment needs, easy operation, can effectively reduce the operation risk.

Description

Sterile experimental animal excrement collecting device

Technical Field

The utility model belongs to the technical field of animal experiment utensil and specifically relates to an aseptic collection device of experimental animals excrement and urine.

Background

The intestinal flora is a hot spot of the current biomedical research, and plays an important role in the occurrence and prevention of intestinal diseases, tumors, nervous system diseases and other diseases. Collecting fresh feces is the first condition for studying intestinal flora so as to further extract and separate bacteria from the feces. Therefore, the feces collection needs aseptic operation, and the influence on the experimental result caused by the mixed foreign bacteria in the environment in the operation sample is avoided. In addition, some studies require extraction of DNA, RNA, etc. from stool for further analysis. It is therefore also desirable to maintain a low temperature environment during fecal collection to slow the degradation of DNA and RNA.

The current methods for collecting animal feces include the following:

one is to disinfect the anus with alcohol and stimulate the animal to defecate by abdominal massage, and to collect the feces with sterile EP tube. However, this method is limited in that: the collection efficiency is slow and the animal can be controlled for a limited time, so that only a small amount of sample can be collected at a time; the collection process is complex, two persons are often needed for operation, one person controls animals, and the other person collects the animals; in the collection process, a collector is easy to touch a sample by mistake to cause pollution; the collection process can not be kept at low temperature, which is not beneficial to extracting DNA and RNA; the operation process is very irritating to animals, and the animals are easy to resist and hurt people.

Another approach is to use a special metabolic study cage that can automatically collect feces, but metabolic study cages are expensive and sterility and low temperature cannot be guaranteed during collection. In addition, the plastic bag is tied to the anus of the animal, and the excrement of the animal can directly fall into the plastic bag. But then the feces and urine will be discharged together into the plastic bag and the sterility requirements cannot be met either. In addition, this method cannot be kept at low temperature, and is disadvantageous for extraction of DNA and RNA.

Chinese patent documents: CN203467379U discloses a laboratory mouse metabolism cage, which comprises a bracket, a mouse cage fixed on the bracket and a separation collector, wherein the bottom of the mouse cage is a net grid, the separation collector is arranged below the mouse cage, the separation collector comprises a funnel, and the size of the upper opening of the funnel is matched with the size of the net grid; the lower end of the conical separator is in an opening design, the conical separator is arranged in the funnel and is coaxial with the funnel, the size of an upper opening of the conical separator is smaller than that of a lower opening of the conical separator, and the size of the lower opening of the conical separator is larger than that of a neck of the funnel; the upper surface of the urine collecting ring is provided with an annular urine collecting groove, the lower opening of the funnel is positioned in the urine collecting groove, the urine collecting ring is obliquely arranged, the lowest end of the urine collecting ring is provided with a urine guide opening, and a urine tube for containing urine is arranged below the urine guide opening; excrement and urine pipe sets up in the funnel below for connect excrement and urine greatly, urine pipe upper end opening part is provided with filtration membrane, can hold back excrement and urine, and the effect of letting excrement and urine separation is more obvious, and it is used for solving the not thorough problem of experiment rat excrement and urine separation, but it does not separate urine and excrement and urine from the source, but separates the urine excrement and urine that has cross contamination through filtration membrane, therefore it can not realize aseptic collection faecal effect.

In summary, there is a need for an experimental animal feces sterile collection device that can separate feces from urine during collection, avoid contact between feces and urine, effectively prevent feces from being contaminated, provide a low-temperature experimental environment favorable for extraction of DNA, RNA and other special experimental needs, and is simple in operation and capable of effectively reducing experimental cost and risks.

Disclosure of Invention

The utility model aims at providing a can separate excrement and urine at the collection in-process, avoid the contact of excrement and urine, prevent effectively that excrement and urine from being contaminated, and can provide the low temperature experimental environment that does benefit to special experiments needs such as extraction DNA, RNA, easy operation, the aseptic collection device of experimental animal excrement and urine of effective reduction experiment cost and risk.

In order to achieve the purpose, the utility model adopts the technical proposal that:

an experimental animal excrement sterile collection device comprises a workbench, a plurality of fixing belts and a liquid absorption device; the workbench comprises a drain hole, a connecting pipe column and an absorption device groove; the connecting pipe column is connected with the lower end of the drain hole, and the other end of the connecting pipe column can be detachably connected with an external collecting container; the absorption device groove is arranged on the workbench, the edge area of the absorption device groove is connected with the drainage hole, and the liquid absorption device is detachably connected with the absorption device groove; the fixed band pass through connecting portion with its surface of workstation is detachable links to each other.

As a preferred technical scheme, the workbench is of a suspended plate-shaped body structure provided with a plurality of supporting columns.

As a preferable technical scheme, the absorption device groove is provided with an absorption window which is arranged next to the drainage hole; the absorption window can be provided with absorbent paper.

As a preferable technical solution, the liquid absorbing device is a water absorbing sponge.

As a preferred technical scheme, the connecting part is of a magic tape structure.

As a preferred technical scheme, the fixing belts comprise a head and neck fixing belt, a chest and back fixing belt and a hip fixing belt; and the hip fixing belt is arranged above the excretion hole.

As a preferred solution, the outer collection container comprises a sterile collector with a sterile cap.

As a preferable technical scheme, a low-temperature storage device can be placed under the drain hole on the workbench.

As a preferred technical solution, the low temperature storage device includes an ice box or a dry ice box, and may provide a low temperature environment for the external collection container.

As a preferred embodiment, the outer collection container may also be a centrifuge tube.

The utility model has the advantages that:

1. the workbench comprises a drain hole and an absorption device groove; the absorbing device groove is arranged on the workbench, the edge area of the absorbing device groove is connected with the drain hole, the urine and the excrement can be separated through the liquid absorbing device in the absorbing device groove, the contact between the excrement and the urine is avoided, and the excrement of the experimental animal only entering the external collecting container is guaranteed.

2. Can place low temperature storage device under the drain hole on the workstation, wherein can be arranged in to outside collecting vessel to special experiment needs are used for providing the low temperature and preserve the environment, and then has made things convenient for the experimenter to extract DNA and RNA from animal manure.

3. This device convenient to use, single can operate, and do benefit to the disinfection cleanness, can disinfect workstation, escape orifice, animal anus before beginning to gather to use aseptic collector, guarantee to collect the sample under the aseptic condition, thereby reduce miscellaneous fungus and pollute.

4. The accessible is a plurality of in the collection process the animal is fixed to the connection dismantled between fixed band and the workstation, need not amazing animal, reduces operating personnel and is bitten the risk by the animal, and does benefit to a large amount of collection samples in a period.

Drawings

FIG. 1 is a schematic view of the components of the experimental animal excrement sterile collecting device of the utility model.

Figure 2 is a side view of the sterile experimental animal excrement collecting device matched with the sterile collector.

Fig. 3 is a schematic perspective view of the experimental animal waste sterile collecting device of the present invention.

Figure 4 is a side view of the sterile collecting device of experimental animal waste and the low-temperature storage device.

FIG. 5 is a side view of the sterile experimental animal waste collecting device in cooperation with a commercially available centrifuge tube.

Detailed Description

The present invention will be further described with reference to the following detailed description. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Furthermore, it should be understood that various changes and modifications can be made by those skilled in the art after reading the disclosure of the present invention, and such equivalents also fall within the scope of the appended claims.

The reference numerals and components referred to in the drawings are as follows:

1. working table 2, fixing belt 3, liquid absorbing device

4. External collecting container 5, cryogenic storage device 11, support column

12. A drain hole 13, a connecting pipe column 14, and an absorption device groove

15. Connecting part 21, head and neck fixing strap 22, chest and back fixing strap

23. Hip fixing belt 41, sterile cap 42, sterile collector

43. Centrifuge tube 141, absorption window 31, water absorption sponge

32. Water-absorbing paper

Example 1

Please refer to fig. 1 and fig. 2; fig. 1 is the component schematic diagram of the experimental animal excrement sterile collecting device of the utility model, and fig. 2 is the side view of the experimental animal excrement sterile collecting device and the sterile collector. An experimental animal excrement sterile collection device comprises a workbench 1, a plurality of fixing belts 2 and a liquid absorption device 3; the workbench 1 is a suspended plate structure provided with a plurality of supporting columns 11 and comprises a drain hole 12, a connecting pipe column 13 and an absorption device groove 14; the connecting pipe column 13 is connected with the lower end of the drain hole 12, and the other end of the connecting pipe column can be detachably connected with the external collecting container 4; the absorption device groove 14 is arranged on the workbench 1, the edge area of the absorption device groove is connected with the drainage hole 12, the liquid absorption device 3 is detachably connected with the absorption device groove, and the liquid absorption device 3 is a water absorption sponge 31; the absorption device groove 14 is provided with an absorption window 141 which is arranged close to the drainage hole 12, and absorbent paper 32 can be placed on the absorption device groove, so that the upper end surface of the absorption device groove 14 filled with the absorbent sponge 31 and the absorbent paper 32 is flush with the upper end plane of the workbench 1; the upper plane of the workbench 1 is also provided with a connecting part 15 which is used for being in adaptive connection with the fixing belt 2; the fixing belts 2 comprise a head and neck fixing belt 21, a chest and back fixing belt 22 and a hip fixing belt 23; the hip fixing strap 23 is arranged above the excretory hole 12 and used for fixing the hip region of the experimental subject, the chest and back fixing strap 22 is used for fixing the chest and back region of the experimental subject, and the head and neck fixing strap 21 is used for fixing the head and neck region of the experimental subject; the head and neck fixing band 21, the chest and back fixing band 22 and the hip fixing band 23 can be detachably connected with the surface of the workbench 1 through a connecting part 15, and the connecting part 15 is of a magic tape structure; the outer collection container 4 is a sterile collector 42 with a sterile cap 41, said sterile cap 41 being removably connected to the sterile collector 42.

Please refer to fig. 3; fig. 3 is a schematic perspective view of the experimental animal waste sterile collecting device of the present invention. The utility model discloses a use method: the liquid absorbing device 3, namely the water absorbing sponge 31 is inserted into the absorbing device groove 14, and then the water absorbing paper 32 is placed in the absorbing window 141; preparing the head and neck fixing straps 21, the chest and back fixing straps 22 and the hip fixing straps 23; anaesthetizing the experimental animal by using anaesthetic drugs which do not influence the intestinal flora, placing the experimental animal on a workbench 1, and enabling the animal anus to be positioned above the excretion hole 12 and the animal urethra opening to be positioned on the absorbent paper 32; then the neck fixing band 21 is used for fixing the neck of the animal; the chest and back fixing belts 22 are used for fixing the chest and back of an animal; the hip fixing belt 23 is used for fixing the hip of an animal; alcohol is sprayed to the bottom surface of the workbench 1 and the anus of the animal for disinfection, after the sprayed alcohol is dried, the sterile collector 42 is taken, the sterile cap 41 on the sterile collector 42 is taken down, then the sterile collector 42 is connected to the connecting pipe column 13, after the collection is finished, the sterile collector 42 is taken down, the sterile cap 41 is covered, and the feces sterile collection is finished.

It should be noted that: the workbench 1 is a suspended plate-shaped body structure provided with a plurality of support columns 11, a low-temperature storage device 5 can be placed below the workbench, and a low-temperature storage environment is provided for the external collection container 4; the absorption window 141 is arranged on the absorption device groove 14 and is close to the drainage hole 12, the absorption window 141 enables the liquid absorption device 3 to directly contact the opening of the urethra of the experimental subject, so that urine is effectively prevented from diffusing, and the absorption window 141 can be provided with absorbent paper 32, so that the absorption strength can be further increased; the fixing belt 2 comprises a head and neck fixing belt 21, a chest and back fixing belt 22 and a hip fixing belt 23, and can stably fix an experimental object on the workbench 1, and the hip fixing belt 23 is arranged above the excretion hole 12 to fix the position of the anus, so that the excrement is ensured to directly fall into the external collection container 4, and the phenomenon that the anus of the experimental object is displaced due to struggling to cause pollution to the excrement and influence the experimental process is avoided; a low-temperature storage device 5 can be placed below a drain hole 12 on the workbench 1, the low-temperature storage device 5 comprises an ice box or a dry ice box, and the outer collection container 4 can be placed in the low-temperature storage device and can be used for the collection process and the low-temperature storage required by transfer or storage for special experiments; the outer collection container 4 includes a sterile collector 42 with a sterile cap 41 or a commercially available centrifuge tube 43, but it is understood that the outer collection container 4 is available from the prior art or commercially available and includes, but is not limited to, the above two collection devices.

Example 2

Please refer to fig. 4; figure 4 is a side view of the sterile collecting device of experimental animal waste and the low-temperature storage device. This embodiment is substantially the same as embodiment 1, except that a low temperature storage device 5 is placed below the drain hole 12 on the worktable 1, and a sterile collector 42 connected to the connecting string 13 is located inside the ice box to provide a low temperature collection environment for the sterile collector 42, it should be noted that the arrangement of the low temperature storage device 5 does not affect the existing practical performance of the device, and the collection process can be maintained at the low temperature collection environment, which is beneficial for extracting DNA and RNA to meet the special experimental requirements.

Example 3

Please refer to fig. 5; FIG. 5 is a side view of the sterile experimental animal waste collecting device in cooperation with a commercially available centrifuge tube. This example is substantially the same as example 1, except that a commercially available centrifuge tube 43 is used as the outer collection container 4, and the connecting column 13 is connected to the centrifuge tube 43; it should be noted that the change of the external collection container 4 does not affect the use efficiency, and the connecting column 13 can be connected with a commercially available centrifuge tube 43, thereby improving the operability of the experiment and reducing the cost.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of improvements and additions can be made without departing from the principles of the present invention, and these improvements and additions should also be regarded as the protection scope of the present invention.

Claims

1. An experimental animal excrement sterile collection device is characterized by comprising a workbench, a plurality of fixing belts and a liquid absorption device; the workbench comprises a drain hole, a connecting pipe column and an absorption device groove; the connecting pipe column is connected with the lower end of the drain hole, and the other end of the connecting pipe column can be detachably connected with an external collecting container; the absorption device groove is arranged on the workbench, the edge area of the absorption device groove is connected with the drainage hole, and the liquid absorption device is detachably connected with the absorption device groove; the fixed band pass through connecting portion with its surface of workstation is detachable links to each other.

2. The sterile experimental animal excrement collecting device according to claim 1, wherein said working table is a suspended plate structure provided with a plurality of supporting columns.

3. The sterile experimental animal excrement collecting device according to claim 1, wherein said absorption device tank is provided thereon with an absorption window disposed adjacent to said drain hole; the absorption window can be provided with absorbent paper.

4. The sterile experimental animal excrement collection device according to claim 1, wherein said liquid absorption device is a water-absorbing sponge.

5. The sterile experimental animal excrement collecting device according to claim 1, wherein the connecting part is of a magic tape structure.

6. The sterile experimental animal excrement collecting device according to claim 1, wherein said fixing straps comprise a head and neck fixing strap, a chest and back fixing strap and a hip fixing strap; and the hip fixing belt is arranged above the excretion hole.

7. The sterile experimental animal waste collection device according to claim 1, wherein said outer collection container comprises a sterile collector with a sterile cap.

8. The sterile experimental animal excrement collecting device according to claim 7, wherein a low-temperature storage device can be placed under the drain hole on the worktable.

9. The sterile experimental animal waste collection device according to claim 8, wherein said low temperature storage device comprises an ice box or a dry ice box, which can provide a low temperature environment for said outer collection container.

10. The sterile experimental animal waste collection device according to claim 1, wherein said outer collection container is a centrifuge tube.