CN212102865U

CN212102865U - Culture apparatus for primary cells of tumor

Info

Publication number: CN212102865U
Application number: CN202020581810.1U
Authority: CN
Inventors: 韩汶延
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-04-18
Filing date: 2020-04-18
Publication date: 2020-12-08
Anticipated expiration: 2030-04-18

Abstract

The utility model provides a primary culture apparatus for cell of tumour, include: the box body, the door body, the culture partition plate and the atomization device are provided with openings at one side; the door body rotates in the opening of the box body; the culture partition plate is detachably arranged in the box body; the atomizing chamber is arranged between the inner side wall of the box body and the outer side wall of the box body, the inner side wall is provided with a through hole communicated with the atomizing chamber, and the atomizing device is communicated with the atomizing chamber through a conduit. The utility model provides a primary culture apparatus for cell of tumour through set up the atomizing chamber between the inside wall and the lateral wall of box, and the fog that atomizing device produced is transmitted to the atomizing intracavity, and in the through-hole that the fog was passed through on the inner wall of incubator entered into the incubator, the through-hole of equipartition not only can increase the humidity of internal gas of incubator to can guarantee the homogeneity of internal gas humidity of box.

Description

Culture apparatus for primary cells of tumor

Technical Field

The utility model relates to a tumour culture apparatus technical field particularly, relates to a primary for cell culture apparatus of tumour.

Background

The primary cell is a cell which is cultured immediately after being taken out from a body, and the cultured 1 st generation cell and cells within 10 generations are generally called primary cell culture, and the primary cell has an irreplaceable function in the field of biomedicine. In the current biomedical field, the research on tumor cells is mostly completed by culturing the tumor primary cells, which have high requirements on the culture environment. However, the humidity environment of the prior art tumor primary cell culture device is poor, and the temperature of the humidity cannot be accurately adjusted, so that the tumor primary cell culture is affected to a certain extent. The utility model provides a primary for cell culture apparatus of tumour is used for solving the technical problem that prior art's incubator appears above.

SUMMERY OF THE UTILITY MODEL

The present invention aims at least solving one of the technical problems existing in the prior art or the related art.

In view of this, the present invention provides a culture device for primary tumor cells.

In order to achieve the above object, the technical solution of the present invention provides a culture apparatus for primary tumor cells, including: the box body, the door body, the culture partition plate and the atomization device are provided with openings at one side; the door body rotates in the opening of the box body; the culture partition plate is detachably arranged in the box body; the atomizing chamber is arranged between the inner side wall of the box body and the outer side wall of the box body, the inner side wall is provided with a through hole communicated with the atomizing chamber, and the atomizing device is communicated with the atomizing chamber through a conduit.

In the above technical solution, preferably, the through holes are arranged in a plurality of rows in the vertical direction, and the distance between any two adjacent rows of through holes is equal.

In any of the above technical solutions, preferably, each row of through holes are sequentially arranged along the horizontal direction, and the distance between two adjacent through holes is equal.

In any of the above technical solutions, preferably, the bottom wall of the box body is inclined downwards gradually from front to back, and a drain hole is formed at the lowest point of the bottom wall of the box body.

In any of the above technical solutions, preferably, the method further includes: the hygrograph is fixedly arranged on the box body and used for detecting the humidity in the box body.

In any of the above technical solutions, preferably, the atomizing device includes: the water tank is provided with a liquid inlet; and the atomizer is fixedly arranged on the water tank, a water inlet of the atomizer extends into the water tank, and a mist outlet of the atomizer is communicated with the atomizing cavity through a conduit.

In any of the above technical solutions, preferably, the atomizing device further includes: the water level gauge is fixedly arranged on the water tank.

In any of the above technical solutions, preferably, an electric heating wire is disposed in the side wall of the water tank.

In any of the above technical solutions, preferably, the atomizing device further includes: and the ultraviolet lamp is fixedly arranged in the water tank and is positioned at the top of the water tank.

Adopt the utility model provides a technical scheme compares with known public technique, has following beneficial effect:

1. the utility model discloses a set up the atomizing chamber between the inside wall and the lateral wall of box, the fog that atomizing device produced is transmitted to the atomizing intracavity, and in the through-hole that the fog passed through on the inner wall of incubator box entered into the incubator, the through-hole of equipartition not only can increase the humidity of internal gas of incubator to can guarantee the homogeneity of internal gas humidity of box.

2. The atomizing chamber can also play the effect that separates the temperature, is convenient for keep the temperature invariant in the box, and then is convenient for the smooth culture of primary tumor cell.

3. The utility model discloses a reserve the wash port in the lower of incubator bottom wall, can in time discharge the incubator with the water droplet of cultivateing in-process liquefaction outside, avoid polluting the cultivation environment.

4. The utility model discloses an increase the design of electric heating wire and liquid reserve tank, through intelligent heating device control electric heating wire, can heat the pure water of storing in the liquid reserve tank to cultivateing required specific temperature, and then guarantee that the fog that the atomizer produced can reach the temperature of specific demand.

5. The utility model discloses an increase the design of ultraviolet fluorescent tube, carry out ultraviolet sterilization through the ultraviolet fluorescent tube to the pure water of storing in the liquid reserve tank.

Additional aspects and advantages of the invention will be set forth in part in the description which follows, or may be learned by practice of the invention.

Drawings

Fig. 1 is a schematic structural view of a culture apparatus for tumor primary cells according to an embodiment of the present invention;

FIG. 2 is a sectional view of a part of the structure of a culture apparatus for tumor primary cells according to an embodiment of the present invention;

fig. 3 is a sectional view of a part of the structure of a culture apparatus for tumor primary cells according to an embodiment of the present invention.

Wherein, the correspondence between the reference numbers and the component names in fig. 1 to 3 is:

10 boxes, 11 inside walls, 111 through holes, 12 outside walls, 13 atomizing cavities, 14 bottom walls, 15 drain holes, 20 door bodies, 30 culture partition plates, 40 atomizing devices, 41 water tanks, 411 liquid inlets, 412 electric heating wires, 42 atomizers, 43 water level meters, 44 ultraviolet lamps, 50 hygrometers and 60 guide pipes.

Detailed Description

In order that the above objects, features and advantages of the present invention can be more clearly understood, a more particular description of the invention will be rendered by reference to the appended drawings, which are illustrated in the appended drawings. It should be noted that the embodiments and features of the embodiments of the present application may be combined with each other without conflict.

In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, however, the present invention may be practiced in other ways than those specifically described herein, and therefore the scope of the present invention is not limited by the specific embodiments disclosed below.

Some embodiments according to the invention are described below with reference to fig. 1 to 3.

As shown in fig. 1 to 3, an embodiment of the present invention provides a culture device for tumor primary cells, including: a box body 10 with one side opened, a door body 20, a culture partition plate 30 and an atomization device 40; the door body 20 rotates in the opening of the box body 10; the culture partition plate 30 is detachably arranged in the box body 10 (for example, a bulge is arranged on the inner side wall 11 of the box body 10, and the culture partition plate 30 is lapped on the bulge); an atomizing chamber 13 is arranged between the inner side wall 11 of the box body 10 and the outer side wall 12 of the box body 10, a through hole 111 communicated with the atomizing chamber 13 is arranged on the inner side wall 11, and the atomizing device 40 is communicated with the atomizing chamber 13 through a conduit 60.

In the above embodiment, preferably, the through holes 111 are arranged in a plurality of rows in the vertical direction, and the pitch between any two adjacent rows of the through holes 111 is equal.

In any of the above embodiments, preferably, each row of through holes 111 is sequentially arranged along the horizontal direction, and the distance between two adjacent through holes 111 is equal.

In this scheme, through set up atomizing chamber 13 between the inside wall 11 and the lateral wall 12 of box 10, the fog that atomizing device 40 produced is transmitted to atomizing chamber 13 in, and the fog enters into culture box 10 through-hole 111 on the inside wall 11 of culture box 10 in, and the through-hole 111 of equipartition not only can increase the humidity of the interior gas of culture box 10 to can guarantee the homogeneity of the interior gas humidity of box 10.

Meanwhile, the atomization cavity 13 can also play a role in thermal insulation, so that the temperature in the box body 10 is kept constant, and smooth culture of primary tumor cells is facilitated.

In any of the above embodiments, preferably, the bottom wall 14 of the box 10 is inclined gradually downwards from front to back, and the lowest point of the bottom wall 14 of the box 10 is provided with the drainage hole 15.

In this scheme, through reserving wash port 15 in 14 lowest departments of incubator diapire, can in time discharge the incubator with the water droplet of cultivateing in-process liquefaction outside, avoid polluting the cultivation environment.

Further preferably, the bottom of the atomizing chamber 13 is inclined with the bottom wall 14 of the housing 10, and a water leakage hole is provided at the lowest point of the atomizing chamber 13.

In this scheme, set up the hole that leaks through the bottom at atomizing chamber 13, can reduce the water droplet in atomizing chamber 13 and remain, and then reduce the possibility that bacterial growing.

In any of the above embodiments, preferably, the method further includes: and the humidity meter 50 is fixedly arranged on the box body 10, and the humidity meter 50 is used for detecting the humidity in the box body 10.

In any of the above embodiments, preferably, the atomizing device 40 includes: a water tank 41, wherein a liquid inlet 411 is arranged on the water tank 41; and the atomizer 42 is fixedly arranged on the water tank 41, a water inlet of the atomizer 42 extends into the water tank 41, and a mist outlet of the atomizer 42 is communicated with the atomizing cavity 13 through a conduit 60.

In any of the above embodiments, preferably, the atomizing device 40 further includes: and a water level gauge 43 fixedly arranged on the water tank 41.

In any of the above embodiments, preferably, an electric heating wire 412 is provided in the side wall of the water tank 41.

In this scheme, through the design that increases electric heating wire 412 and liquid reserve tank, through intelligent heating device control electric heating wire 412, can heat the pure water of storing in the liquid reserve tank to the required specific temperature of cultivateing, and then guarantee that the fog that atomizer 42 produced can reach the temperature of specific demand.

In any of the above embodiments, preferably, the atomizing device 40 further includes: and the ultraviolet lamp 44 is fixedly arranged in the water tank 41 and is positioned at the top of the water tank 41.

In this scheme, through the design that increases the ultraviolet fluorescent tube, carry out ultraviolet sterilization through the ultraviolet fluorescent tube to the pure water that stores in the liquid reserve tank.

DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION

When the device is used, firstly, a culture dish or a culture bottle of the primary tumor cells to be cultured is placed on the culture partition plate 30, and then the door body 20 is closed to perform constant-temperature culture on the primary tumor cells; detect the humidity of gaseous in the cultivation box 10 through hygrograph 50, when the humidity in the cultivation box 10 needs to be increased, the pure water after will being sterilized by ultraviolet lamp 44 through intelligent heating device control electric heating wire 412 heats to required temperature, then switch on atomizer 42, atomize the sterilization pure water of specific temperature in the water tank 41, the fog after the atomizing passes through fog steam pipe 60 and enters into atomizing chamber 13, the rethread through-hole 111 enters into in the cultivation box 10, and then can the homogeneity increase cultivate the humidity of gaseous in the box 10, and can guarantee the temperature of fog steam. After the mist is liquefied, water drops can be discharged from the inner cavity of the culture box body 10 in real time through the water discharge holes 15 on the bottom wall 14 of the culture box body 10, and the culture environment is guaranteed not to be polluted.

Wherein, the box body 10 is connected with a vent pipe for carbon dioxide flowing, the vent pipe is connected with a carbon dioxide source, the vent pipe is provided with a valve, when the valve is opened, the inside of the box body 10 is communicated with the carbon dioxide source so as to supply carbon dioxide into the box body 10.

Wherein the carbon dioxide source is a carbon dioxide generator.

The utility model has the advantages of being simple in structure and easy and simple to handle and manufacturing cost is lower, can enough carry out even regulation and guarantee the accurate temperature of humidification fog to the humidity in the incubator, can guarantee the cultivation environment in the incubator again.

In the present application, the terms "first", "second", "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance; the term "plurality" means two or more unless expressly limited otherwise. The terms "mounted," "connected," "fixed," and the like are to be construed broadly, and for example, "connected" may be a fixed connection, a removable connection, or an integral connection; "coupled" may be direct or indirect through an intermediary. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In the description of the present invention, it should be understood that the terms "upper", "lower", "left", "right", "front", "back", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or unit indicated must have a specific direction, be constructed and operated in a specific orientation, and therefore, should not be construed as limiting the present invention.

In the description of the present specification, the description of the terms "one embodiment," "some embodiments," "specific embodiments," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A culture apparatus for primary tumor cells, comprising:

a box body (10) with an opening at one side, a door body (20), a culture partition plate (30) and an atomization device (40);

the door body (20) rotates in the opening of the box body (10);

the culture partition plate (30) is detachably arranged in the box body (10);

the utility model discloses a spray atomizer, including box (10), the inside wall (11) of box (10) with atomizing chamber (13) have between the lateral wall (12) of box (10), be equipped with on inside wall (11) with through-hole (111) of atomizing chamber (13) intercommunication, atomizing device (40) with atomizing chamber (13) pass through pipe (60) intercommunication.

2. The device for culturing tumor primary cells according to claim 1,

the through holes (111) are arranged in multiple rows in the vertical direction, and the distance between any two adjacent rows of the through holes (111) is equal.

3. The device for culturing tumor primary cells according to claim 2,

every row through-hole (111) set gradually along the horizontal direction, and adjacent two the interval is equal between through-hole (111).

4. The device for culturing tumor primary cells according to claim 1,

from front to back, the bottom wall (14) of the box body (10) is gradually inclined downwards, and a drain hole (15) is formed in the lowest point of the bottom wall (14) of the box body (10).

5. The device for culturing tumor primary cells according to any one of claims 1 to 4, further comprising:

the humidity meter (50) is fixedly arranged on the box body (10), and the humidity meter (50) is used for detecting the humidity in the box body (10).

6. The culture device for tumor primary cells according to any of claims 1 to 4, wherein the nebulizing means (40) comprise:

the water tank (41), wherein a liquid inlet (411) is arranged on the water tank (41); and

the atomizer (42) is fixedly arranged on the water tank (41), a water inlet of the atomizer (42) extends into the water tank (41), and a mist outlet of the atomizer (42) is communicated with the atomizing cavity (13) through the conduit (60).

7. The device for culturing tumor primary cells according to claim 6, wherein the nebulizing means (40) further comprises:

and the water level gauge (43) is fixedly arranged on the water tank (41).

8. The device for culturing tumor primary cells according to claim 6,

an electric heating wire (412) is arranged in the side wall of the water tank (41).

9. The device for culturing tumor primary cells according to claim 6, wherein the nebulizing means (40) further comprises:

and the ultraviolet lamp (44) is fixedly arranged in the water tank (41) and is positioned at the top of the water tank (41).