CN212077019U

CN212077019U - Extraction element is used in nucleic acid testing

Info

Publication number: CN212077019U
Application number: CN202020441706.2U
Authority: CN
Inventors: 曾丹; 叶建方; 苑洁
Original assignee: Guizhou Center For Disease Control And Prevention
Current assignee: Guizhou Center For Disease Control And Prevention
Priority date: 2020-03-31
Filing date: 2020-03-31
Publication date: 2020-12-04
Anticipated expiration: 2030-03-31

Abstract

The utility model discloses an extraction device for nucleic acid detection, relating to the technical field of nucleic acid extraction, which comprises a box body, wherein a plurality of extraction chambers are arranged inside the box body; the utility model discloses a box, draw the room, the case lid, the activity cavity, drive mechanism, shake equal stick, the collecting box, the outage, the chamber slides, the setting of closing plate and screw rod, it needs manual extraction when many times to have solved current nucleic acid detection, but manual extraction mostly can only draw a sample at every turn, when a plurality of samples draw, extraction efficiency is low, it consumes longer to draw, the waste liquid after drawing simultaneously lacks centralized collection and handles, all need topple over at every turn, very troublesome problem, possess the advantage of manual extraction and the waste liquid collection of being convenient for of a plurality of samples simultaneously, effectively reduced and drawn personnel's repetitive operation number of times, the operating efficiency of manual extraction nucleic acid has been improved, and conveniently concentrate emission treatment, therefore, the multifunctional integrated circuit board is very practical, and is worth using widely.

Description

Extraction element is used in nucleic acid testing

Technical Field

The utility model relates to a nucleic acid extraction technical field specifically is an extraction element for nucleic acid testing.

Background

At present, nucleic acid is the most important substance in all biological molecules, widely existing in all animal and plant cells and microorganisms, and is a general term for deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). Based on the development of biotechnology, nucleic acid detection technology is widely used in various fields, such as pathogen biological detection, substance identification, genetic relationship, etc., and plays a very important role, but these researches require that nucleic acid is separated and extracted before detection.

Through search discovery, the Chinese utility model patent: application No.: CN201520132302.4, publication No.: CN204607994U, a nucleic acid rapid extraction device, comprising a centrifugal column, wherein the centrifugal column comprises two independent cavities formed by separation, the independent cavities comprise a first cavity and a second cavity, and a vertical separation body and a horizontal separation body are arranged between the first cavity and the second cavity and divide the first cavity and the second cavity into independent cavities; the centrifugal column is also provided with a microporous filter plate and a nucleic acid adsorption membrane positioned below the microporous filter plate; the horizontal separating body is provided with a liquid outlet hole communicated with the second cavity; the top of the centrifugal column is provided with an opening respectively connected with the first cavity and the second cavity, and the inner side of the bottom of the centrifugal column is provided with the nucleic acid adsorption film. The technical scheme of the utility model, at the nucleic acid extraction in-process to trace (like forensic identification) sample, can effectively avoid causing the loss of trace nucleic acid material evidence because of the absorption of examining material such as swab, cotton swab, essence spot, blood spot, cigarette butt and the absorption of centrifugation pipe wall, improve nucleic acid extraction efficiency, reduce cross contamination simultaneously.

In summary, the existing nucleic acid detection needs manual extraction many times, but manual extraction mostly only can extract one sample at a time, when a plurality of samples are extracted, the extraction efficiency is low, the extraction consumption is long, and meanwhile, the extracted waste liquid is lack of centralized collection treatment and needs to be dumped every time, which is very troublesome, so that an extraction device for nucleic acid detection, which has the advantages that a plurality of samples are simultaneously extracted manually and the waste liquid is convenient to collect, is provided.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide an extraction element for nucleic acid testing possesses the advantage that a plurality of samples were manual simultaneously and were drawed and the waste liquid of being convenient for is collected, has solved current nucleic acid testing and need manual drawing many times, but manual drawing mostly can only draw a sample at every turn, when a plurality of samples were drawed, extraction efficiency was low, and it is longer to draw, and the waste liquid after drawing simultaneously lacks centralized collection processing, all need topple over at every turn, very troublesome problem.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides an extraction element for nucleic acid testing, includes the box, the inside of box has been seted up a plurality of and has been drawed the room, the top joint of box has the case lid, movable cavity has been seted up to the inside of case lid, the internally mounted of activity cavity has drive mechanism, drive mechanism's bottom is run through to the bottom of case lid and is located to draw the inside of room and has cup jointed and shake the stick all, the bottom of box has bolted the collecting box, draw the bottom of room and seted up the outage, and the bottom of outage communicates with the top of collecting box, the inside of box has been located one side of outage and has been seted up the chamber of sliding, the inside sliding connection in chamber of sliding has the closing plate, and the closing plate is sealed with the outage, the left side rotation of closing plate.

Preferably, the transmission mechanism comprises a first gear, a first rotating shaft is sleeved at the center of the first gear and is rotatably connected with the movable cavity, the bottom of the first rotating shaft is sleeved with the top of the shaking rod through a bearing, the inner sides of the two first gears positioned on the front side are meshed with a second gear, a second rotating shaft is sleeved at the center of the second gear and is rotatably connected with the movable cavity.

Preferably, the top of the second rotating shaft penetrates through the top of the box cover through a bearing and is bolted with a rocking handle, and a protective sleeve is adjusted on the surface of the rocking handle.

Preferably, the bottom of the inner side of the extraction chamber is provided with a filter plate, a porous nucleic acid adsorption medium is placed in the extraction chamber, and the diameter of the porous nucleic acid adsorption medium is larger than the inner diameter of a filter hole of the filter plate.

Preferably, a sealing gasket is bonded to the top of the inner side of the box cover and is matched with the opening of the box body.

Preferably, a handle is bolted to the center of the top of the box cover, and an anti-slip sleeve is sleeved on the surface of the handle.

Preferably, one end of the screw rod, which is far away from the sealing plate, penetrates through the outer side of the box body and is bolted with a handle, and a threaded hole matched with the screw rod for use is formed in the surface of the box body.

Preferably, four corners of the bottom of the collecting box are all bolted with supporting legs, the right side of the collecting box is connected with a liquid discharge pipe, and the surface of the liquid discharge pipe is bolted with a water valve.

Preferably, the surface of the box body is integrally provided with scales, and the scales are matched with the height of the extraction chamber.

Compared with the prior art, the beneficial effects of the utility model are as follows:

the utility model discloses a box, draw room, case lid, activity cavity, drive mechanism and shake the setting of equal stick, it needs manual extraction when many to have solved current nucleic acid testing, but the manual sample that can only be drawed at every turn of drawing mostly, when a plurality of samples draw, extraction efficiency is low, draw and consume longer problem, possess a plurality of samples and manually draw simultaneously, effectively reduced the repetitive operation number of times that draws personnel, improved the manual operation efficiency who draws nucleic acid, and is very practical, and is worth using widely.

The utility model discloses a setting of collecting box, outage, the chamber of sliding, closing plate and screw rod has solved the waste liquid after drawing and has lacked the centralized collection processing, all need topple over at every turn, and is very troublesome, has possessed the advantage that the waste liquid of being convenient for was collected, convenient concentrated emission processing, simple and convenient practicality.

Drawings

FIG. 1 is a front view of the structure of the present invention;

FIG. 2 is a schematic top sectional view of the cover of the present invention;

FIG. 3 is a left side view of the structure of the present invention;

fig. 4 is an enlarged schematic view of a portion a in fig. 1 according to the present invention.

In the figure: 1. a box body; 2. an extraction chamber; 3. a box cover; 4. a movable chamber; 5. a transmission mechanism; 51. a first gear; 52. a first rotating shaft; 53. a second gear; 54. a second rotating shaft; 6. Shaking the uniform rod; 7. a collection box; 8. a drain hole; 9. a sliding cavity; 10. a sealing plate; 11. a screw; 12. a rocking handle; 13. a filter plate; 14. a porous nucleic acid adsorption media; 15. a gasket; 16. a handle; 17. a handle; 18. supporting legs; 19. a liquid discharge pipe; 20. and (4) calibration.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1 to fig. 3, the present invention provides an embodiment: an extraction device for nucleic acid detection comprises a box body 1, a plurality of extraction chambers 2 are arranged in the box body 1, a box cover 3 is clamped at the top of the box body 1, a movable cavity 4 is arranged in the box cover 3, a transmission mechanism 5 is arranged in the movable cavity 4, the bottom of the transmission mechanism 5 penetrates through the bottom of the box cover 3 and is sleeved with a shaking equalizing rod 6 in the extraction chamber 2, the problems that the extraction chamber 2, the box cover 3, the movable cavity 4, the transmission mechanism 5 and the shaking equalizing rod 6 are arranged are solved, manual extraction is needed in many times in the existing nucleic acid detection, but most of manual extraction can only extract one sample at each time, when a plurality of samples are extracted, the extraction efficiency is low, the extraction consumption is long, the simultaneous manual extraction of the samples is realized, the repeated operation times of extraction personnel are effectively reduced, and the operation efficiency of manually extracting nucleic acid is improved, is very practical and is worth popularizing and using.

Further, drive mechanism 5 includes first gear 51, first pivot 52 has been cup jointed to the center department of first gear 51, and first pivot 52 rotates with movable chamber 4 to be connected, and the bottom of first pivot 52 cup joints through bearing and the top of shaking equal stick 6, the inboard meshing of two first gear 51 that are located the front side has second gear 53, second pivot 54 has been cup jointed to the center department of second gear 53, and second pivot 54 rotates with movable chamber 4 to be connected, through setting up first gear 51, first pivot 52, second gear 53 and second pivot 54, effectively realize that a plurality of shake equal stick 6 and shake equal operation simultaneously.

Further, the top of the second rotating shaft 54 penetrates through the top of the box cover 3 through a bearing and is bolted with the rocking handle 12, a protective sleeve is adjusted on the surface of the rocking handle 12, and the second rotating shaft 54 can be conveniently operated manually through the arrangement of the rocking handle 12.

Further, draw the inboard bottom of room 2 and install filter 13, draw the inside of room 2 and placed porous nucleic acid adsorption medium 14, and porous nucleic acid adsorption medium 14's diameter is greater than the filtration pore diameter of filter 13, through setting up filter 13, effectively filters when being convenient for to expel liquid, through setting up porous nucleic acid adsorption medium 14, effectively adsorbs the nucleic acid of schizolysis department.

Further, the top of the inner side of the box cover 3 is bonded with a sealing gasket 15, the sealing gasket 15 is matched with the opening of the box body 1, and the sealing performance of the opening of the box body 1 is effectively enhanced by arranging the sealing gasket 15.

Referring to fig. 1, fig. 2 and fig. 4, the present invention provides an embodiment: the utility model provides an extraction element for nucleic acid testing, the bottom of box 1 has bolted the collecting box 7, draws the bottom of room 2 and has seted up outage 8, and the bottom of outage 8 and the top intercommunication of collecting box 7, and the inside of box 1 has been seted up the chamber 9 that slides and is located one side of outage 8, and the inside sliding connection in chamber 9 that slides has closing plate 10, and closing plate 10 is sealed with outage 8, and the left side of closing plate 10 is rotated and is connected with screw rod 11.

Further, the center of the top of the case cover 3 is bolted with a handle 16, the surface of the handle 16 is sleeved with an anti-slip sleeve, and the case cover 3 is convenient to operate to seal and assist the rocking handle 12 to use by arranging the handle 16.

Further, the one end that the screw rod 11 kept away from the sealing plate 10 runs through to the outside of box 1 and bolted connection has handle 17, and the screw hole that cooperation screw rod 11 used is seted up on the surface of box 1, through setting up handle 17, is convenient for operate screw rod 11 and cooperates sealing plate 10 to seal or flowing back the operation.

Furthermore, the four corners of the bottom of the collecting box 7 are all bolted with supporting legs 18, the right side of the collecting box 7 is connected with a liquid discharge pipe 19, the surface of the liquid discharge pipe 19 is bolted with a water valve, the stability of the device is effectively enhanced by arranging the supporting legs 18, and the liquid discharge pipe 19 is arranged to facilitate the concentrated discharge of waste liquid.

Further, the surface of box 1 is integrative to be processed there is scale 20, and scale 20 and the high looks adaptation that draws room 2, through setting up scale 20, is convenient for observe the nucleic acid and separates out the adsorption condition.

The working principle is as follows: when the device is used, a saline solution is preloaded in the extraction chamber 2 in the box body 1, the saline solution has an auxiliary function of nucleic acid extraction and cracking, a sample is placed in the extraction chamber 2, the box cover 3 is covered by the handle 16, the rocking handle 12 is rotated, the rocking handle 12 drives the second rotating shaft 54, the second rotating shaft 54 drives the second gear 53 in the movable chamber 4, the second gear 53 simultaneously drives the two first gears 51 with meshed surfaces, the first gears 51 drive the other first gears 51 with mutually meshed surfaces, the six first gears 51 simultaneously rotate, the first gear 51 drives the first rotating shaft 52 at the center, the first rotating shaft 52 drives the shaking equalizing rod 6 sleeved at the bottom to stir in the extraction chamber 2, nucleic acid cracking is accelerated, nucleic acid at the cracking position is adsorbed and separated out with the porous nucleic acid adsorption medium 14 in the stirring process, and the porous nucleic acid adsorption medium 14 can be any porous adsorption material such as porous glass beads, graphene particles and the like, after nucleic acid is cracked and desorbed, the handle 17 is rotated, the handle 17 rotates the screw rod 11, the screw rod 11 pulls out the sealing plate 10, the sealing plate 10 is separated from the liquid discharge hole 8, and the waste liquid in the extraction chamber 2 is filtered by the filter plate 13 and then discharged to the inside of the collection box 7 through the liquid discharge hole 8.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. An extraction device for nucleic acid detection, includes box (1), its characterized in that: the improved box is characterized in that a plurality of extraction chambers (2) are arranged in the box body (1), a box cover (3) is clamped at the top of the box body (1), a movable cavity (4) is arranged in the box cover (3), a transmission mechanism (5) is arranged in the movable cavity (4), the bottom of the transmission mechanism (5) penetrates through the bottom of the box cover (3) and is sleeved with a shaking equalizing rod (6) in the extraction chamber (2), a collecting box (7) is bolted at the bottom of the box body (1), a liquid discharge hole (8) is arranged at the bottom of the extraction chamber (2), the bottom end of the liquid discharge hole (8) is communicated with the top of the collecting box (7), a sliding cavity (9) is arranged in one side of the liquid discharge hole (8) in the box body (1), a sealing plate (10) is slidably connected in the sliding cavity (9), and the sealing plate (10) is sealed with the liquid discharge hole (8), the left side of the sealing plate (10) is rotatably connected with a screw rod (11).

2. The extraction device for nucleic acid detection according to claim 1, wherein: drive mechanism (5) include first gear (51), first pivot (52) have been cup jointed to the center department of first gear (51), and first pivot (52) rotate with activity cavity (4) and be connected, and the bottom of first pivot (52) cup joints through bearing and the top of shaking equal stick (6), and the inboard meshing of two first gear (51) that are located the front side has second gear (53), second pivot (54) have been cup jointed to the center department of second gear (53), and second pivot (54) rotate with activity cavity (4) and be connected.

3. The extraction device for nucleic acid detection according to claim 2, wherein: the top of the second rotating shaft (54) penetrates through the top of the box cover (3) through a bearing and is bolted with a rocking handle (12), and a protective sleeve is adjusted on the surface of the rocking handle (12).

4. The extraction device for nucleic acid detection according to claim 1, wherein: the bottom of the inner side of the extraction chamber (2) is provided with a filter plate (13), the inside of the extraction chamber (2) is provided with a porous nucleic acid adsorption medium (14), and the diameter of the porous nucleic acid adsorption medium (14) is larger than the inner diameter of a filter hole of the filter plate (13).

5. The extraction device for nucleic acid detection according to claim 1, wherein: a sealing gasket (15) is bonded to the top of the inner side of the box cover (3), and the sealing gasket (15) is matched with the opening of the box body (1).

6. The extraction device for nucleic acid detection according to claim 1, wherein: the center of the top of the box cover (3) is bolted with a handle (16), and the surface of the handle (16) is sleeved with an anti-slip sleeve.

7. The extraction device for nucleic acid detection according to claim 1, wherein: one end, far away from the sealing plate (10), of the screw rod (11) penetrates through the outer side of the box body (1) and is bolted with a handle (17), and a threaded hole matched with the screw rod (11) for use is formed in the surface of the box body (1).

8. The extraction device for nucleic acid detection according to claim 1, wherein: supporting legs (18) are bolted at four corners of the bottom of the collecting box (7), a liquid discharge pipe (19) is connected to the right side of the collecting box (7), and a water valve is bolted on the surface of the liquid discharge pipe (19).

9. The extraction device for nucleic acid detection according to claim 1, wherein: the surface of the box body (1) is integrally processed with scales (20), and the scales (20) are matched with the height of the extraction chamber (2).