CN219489891U - Antibody purification and separation device - Google Patents

Abstract

The utility model discloses an antibody purifying and separating device which comprises a base, wherein vertical plates are fixedly connected to two sides of the top of the base, a crushing assembly and a separating assembly are sequentially arranged between the two vertical plates from the top to the top, a purifying assembly is arranged at the top of the base and comprises two supporting columns fixedly connected between the two vertical plates, a purifying box is fixedly connected to the top ends of the two supporting columns, and two transverse plates are fixedly connected to the inside of the purifying box. According to the utility model, the electric driving vertical rod is driven to rotate by the driving device, so that the cleaning brush on the vertical rod can clean the cells piled up by the first filter screen and the second filter screen on the two transverse plates, the filtering effect of the first filter screen and the second filter screen is prevented from being influenced by more cells, the device can improve the filtering effect of the first filter screen and the second filter screen, the problem that the cells are piled up and need to be manually treated is effectively avoided, the stability of the purification environment is ensured, and the integral purification efficiency is improved.

Description

Antibody purification and separation device

Technical Field

The utility model relates to the technical field of biology, in particular to an antibody purifying and separating device.

Background

Antibodies are proteins that are produced by the body as a result of stimulation by an antigen and that have a protective effect. It (immunoglobulin is not just an antibody) is a large Y-shaped protein secreted by plasma cells (effector B cells) and used by the immune system to identify and neutralize foreign substances such as bacteria, viruses, etc., found only in body fluids such as vertebrate blood, and the cell membrane surface of B cells thereof. Antibodies recognize a unique feature of a particular foreign object, which is called an antigen.

When the existing antibody purification and separation device is used for purifying an antibody, a series of treatment and filtration are needed to be carried out on the antibody, and when the antibody is filtered, some larger cells are blocked on the filter screen, so that other cells cannot pass through the filter screen, and at the moment, after the filter screen is required to be treated by an operator, when the filter screen is treated by the operator, the pollution is easily brought to the purification environment, and the purification and separation device cannot continue to work while the treatment is carried out, so that the integral purification rate is easily influenced.

Disclosure of Invention

The utility model aims to solve the defects in the prior art and provides an antibody purifying and separating device.

In order to achieve the above purpose, the present utility model adopts the following technical scheme: the utility model provides an antibody purification separator, includes the base, the both sides fixedly connected with riser at base top, two have set gradually broken subassembly and separation subassembly from top to top between the riser, the top of base is provided with purification subassembly, purification subassembly includes two support columns of fixed connection between two risers, two the top fixedly connected with purification case of support column, the inside fixedly connected with diaphragm of purification case, be located the top the inside right side fixedly connected with first filter screen of diaphragm, be located the bottom the inside right side fixedly connected with second filter screen of diaphragm, two the logical groove has all been seted up on the inside left side of diaphragm, two the inside rotation of purification case is connected with same montant, two equal fixedly connected with cleaning brush on the montant, two the equal sliding connection collection box in the left bottom of diaphragm, two the left side fixedly connected with same baffle of collection box.

As a further description of the above technical solution:

the bottom of purifying box is provided with driving motor, driving motor's output rotates runs through purifying box and with the bottom fixed connection of montant.

As a further description of the above technical solution:

the outside of purifying box rotates to be connected with two limiting plates, two limiting plates all with the lateral wall swing joint of baffle.

As a further description of the above technical solution:

the crushing assembly comprises a crushing box fixedly connected between two vertical plates, a first discharging pipe is communicated with the bottom end of the crushing box, and a first switching valve is arranged on the first discharging pipe.

As a further description of the above technical solution:

the separation assembly comprises a separation box fixedly connected between two vertical plates, a second discharging pipe is communicated with the bottom end of the separation box, and a second switching valve is arranged on the second discharging pipe.

As a further description of the above technical solution:

the bottom of the first discharging pipe is communicated with the separation box, and the bottom of the second discharging pipe is communicated with the purification box.

The utility model has the following beneficial effects:

compared with the prior art, this antibody purification separator, through setting up the rotation of drive electric drive montant, and then make the cleaning brush on the montant can clear up first filter screen and the piled up cell of second filter screen on two diaphragms, avoid the cell to influence its filter effect more, when the cleaning brush rotates and advance first filter screen and second filter screen, can drive to pile up the cell on first filter screen and second filter screen and remove, the cleaning brush continues to rotate and brings the cell of one side to the logical groove top of diaphragm opposite side, piled up cell will be transferred into the recovery tank of below through logical groove, the convenience is to it carry out recovery processing, the device can improve the filter effect of first filter screen and second filter screen, the effectual problem that needs manual handling of cell pile up has been avoided, the stability of purification environment is guaranteed and, holistic purification efficiency is improved.

Drawings

FIG. 1 is a schematic diagram of the overall external structure of an antibody purification and separation device according to the present utility model;

FIG. 2 is a longitudinal cross-sectional side view of a part of an antibody purifying and separating apparatus according to the present utility model;

fig. 3 is a cross-sectional top view of a part of the structure of an antibody purification and separation device according to the present utility model.

Legend description:

1. a base; 2. a riser; 3. a support column; 4. a purifying box; 5. a cross plate; 6. a first filter screen; 7. a second filter screen; 8. a through groove; 9. a vertical rod; 10. a cleaning brush; 11. a recovery box; 12. a baffle; 13. a driving motor; 14. a limiting plate; 15. a crushing box; 16. a first discharge pipe; 17. a first switching valve; 18. a separation box; 19. a second discharge pipe; 20. and a second switching valve.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Referring to fig. 1-3, the present utility model provides an antibody purification and separation device: comprises a base 1, two sides of the top of the base 1 are fixedly connected with risers 2, the top of the base 1 is provided with a purification component, the purification component comprises two support columns 3 fixedly connected between the two risers 2, the top ends of the two support columns 3 are fixedly connected with a purification box 4, the purification box 4 is arranged into a cylinder shape, the support columns 3 are used for supporting the purification box 4, the inside of the purification box 4 is fixedly connected with two transverse plates 5, the shape of the transverse plates 5 is consistent with that of the purification box 4, the two transverse plates 5 are distributed up and down, the right side of the inside of the transverse plates 5 at the top is fixedly connected with a first filter screen 6, the right side of the inside of the transverse plates 5 at the bottom is fixedly connected with a second filter screen 7, the first filter screen 6 and the second filter screen 7 are both arranged into a fan shape, the filter eyes of the first filter screen 6 are larger than those of the second filter screen 7, the left side of the inside of the two transverse plates 5 is provided with through grooves 8, the through groove 8 is arranged into a long strip shape, the same vertical rod 9 is rotationally connected inside the two purifying boxes 4, the vertical rod 9 is arranged at the center position of the two transverse plates 5, a driving motor 13 is arranged at the bottom of the purifying box 4, the driving motor 13 is connected with an external power supply and a control switch, the output end of the driving motor 13 rotationally penetrates through the purifying box 4 and is fixedly connected with the bottom end of the vertical rod 9, the cleaning brush 10 is fixedly connected with the two vertical rods 9, the cleaning brush 10 is contacted with the surface of the transverse plates 5, the bottoms at the left sides of the two transverse plates 5 are respectively and slidingly connected with the recovery boxes 11, the same baffle 12 is fixedly connected with the left sides of the two recovery boxes 11, the baffle 12 is arranged to facilitate the disassembly of the two recovery boxes 11, a groove in which the baffle 12 slides is arranged on the purifying box 4, the external rotational connection of the purifying box 4 is fixedly connected with the two limiting plates 14, the two limiting plates 14 are movably connected with the outer side walls of the baffle 12, by providing the limiting plate 14, the baffle plate 12 is fixed.

The utility model provides a set gradually crushing subassembly and separating assembly from top to top between two risers 2, crushing subassembly includes the crushing case 15 of fixed connection between two risers 2, the inside of crushing case 15 is provided with supersonic generator, can carry out the breakage to the cell tissue, the bottom intercommunication of crushing case 15 has first discharging pipe 16, be provided with first ooff valve 17 on the first discharging pipe 16, separating assembly includes the separating case 18 of fixed connection between two risers 2, the inside of separating case 18 is provided with the separator, the bottom intercommunication of separating case 18 has second discharging pipe 19, be provided with second ooff valve 20 on the second discharging pipe 19, the bottom and the separating case 18 intercommunication of first discharging pipe 16, the bottom and the purifying case 4 intercommunication of second discharging pipe 19, first ooff valve 17 and second ooff valve 20 all adopt external control ware to control, and second ooff valve 20 sets up intermittent type ooff valve, intermittent type time is the time that cleaning brush 10 passes through first filter screen 6, then second ooff valve 20 is in the open state, the discharge gate of second discharging pipe 19 sets up in the top of first filter screen 6 on the contrary.

Working principle:

after the antibody to be purified is poured into the crushing box 15 for crushing, the first switch valve 17 on the first discharging pipe 16 is opened, so that the antibody crushed in the crushing box 15 enters the separating box 18 for separation, when the antibody is subjected to the treatment of the separating box 18 and can be filtered, the second switch valve 20 and the driving motor 13 on the second discharging pipe 19 are simultaneously opened, the separated antibody flows into the purifying box 4 through the second discharging pipe 19 and is filtered for the first time through the first filter screen 6 on the top transverse plate 5, after the antibody is filtered through the first filter screen 6, larger cells are left on the first filter screen 6, other substances are transferred to the second filter screen 7 through the first filter screen 6 for secondary filtration, after the antibody is filtered, the cells which cannot pass through the second filter screen 7 are left on the second filter screen 7, the second discharging pipe 19 is the purer antibody passing through the second filter screen 7, while the second discharging pipe 19 circulates, the driving motor 13 drives the vertical rod 9 to rotate, the vertical rod 9 rotates to drive the two cleaning brushes 10 to rotate, when the cleaning brushes 10 are about to enter the first filter screen 6 and the second filter screen 7, the second switch valve 20 on the second discharging pipe 19 is closed, the second discharging pipe 19 does not circulate the antibody liquid, the cleaning brushes 10 enter the first filter screen 6 and the second filter screen 7, the filtered cells on the first filter screen 6 and the second filter screen 7 are pushed, the cells accumulated on the first filter screen 6 and the second filter screen 7 move along with the rotation of the cleaning brushes 10 until the cleaning brushes 10 pass through the first filter screen 6 and the second filter screen 7, the second switch valve 20 is opened again, the second discharging pipe 19 performs blanking filtration again, the cleaning brushes 10 drive the accumulated cells to move to the through groove 8, cells drop to the recovery tank 11 of logical groove 8 bottom through logical groove 8, after the purification, through rotating the outside two limiting plates 14 of purification case 4 for after limiting plate 14 does not contact with baffle 12, pulling baffle 12 makes baffle 12 drive two recovery tanks 11 and removes, is convenient for dismantle recovery tank 11, can handle the inside material of recovery tank 11 can.

Finally, it should be noted that: the foregoing description is only illustrative of the preferred embodiments of the present utility model, and although the present utility model has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described, or equivalents may be substituted for elements thereof, and any modifications, equivalents, improvements or changes may be made without departing from the spirit and principles of the present utility model.

Claims (6)

1. An antibody purification and separation device, comprising a base (1), characterized in that: the utility model discloses a novel purification device, including base (1), riser (2) are connected with in both sides at base (1) top, two from top to top set gradually broken subassembly and separation subassembly between riser (2), the top of base (1) is provided with purification subassembly, purification subassembly includes two support columns (3) of fixed connection between two risers (2), two the top fixedly connected with purification case (4) of support column (3), the inside fixedly connected with two diaphragm (5) of purification case (4), be located the top right side fixedly connected with first filter screen (6) inside diaphragm (5), be located the bottom right side fixedly connected with second filter screen (7) inside diaphragm (5), two the inside left side of diaphragm (5) has all been seted up logical groove (8), two the inside rotation of purification case (4) is connected with same montant (9), two on montant (9) equal fixedly connected with cleaning brush (10), two the bottom of diaphragm (5) is left side first filter screen (11), two equal sliding connection has two recovery case (11) on the same left side.

2. The antibody purification and separation device according to claim 1, wherein: the bottom of purifying box (4) is provided with driving motor (13), the output of driving motor (13) rotates and runs through purifying box (4) and with the bottom fixed connection of montant (9).

3. The antibody purification and separation device according to claim 1, wherein: the outside of purifying box (4) rotates to be connected with two limiting plates (14), two limiting plates (14) all with the lateral wall swing joint of baffle (12).

4. The antibody purification and separation device according to claim 1, wherein: the crushing assembly comprises a crushing box (15) fixedly connected between two vertical plates (2), a first discharging pipe (16) is communicated with the bottom end of the crushing box (15), and a first switch valve (17) is arranged on the first discharging pipe (16).

5. The antibody purification and separation apparatus according to claim 4, wherein: the separation assembly comprises a separation box (18) fixedly connected between the two vertical plates (2), a second discharging pipe (19) is communicated with the bottom end of the separation box (18), and a second switch valve (20) is arranged on the second discharging pipe (19).

6. The antibody purification and separation apparatus according to claim 5, wherein: the bottom end of the first discharging pipe (16) is communicated with the separation box (18), and the bottom end of the second discharging pipe (19) is communicated with the purification box (4).