CN211999718U - Novel PCR temperature control sample application box - Google Patents

Abstract

The utility model relates to a novel PCR accuse temperature point appearance box, box body and lid including the transparent plastic preparation, the box body intussuseption is filled with the heat conduction material piece, be equipped with a plurality of test-tube groove on the heat conduction material piece, the bottom of box body is equipped with the refrigerant groove, the refrigerant inslot is equipped with refrigerant box, refrigerant box is through setting up the opening of box body side is put into the refrigerant inslot, placed the refrigerant in the refrigerant box, the refrigerant with the contact of heat conduction material piece, the heat conduction material piece with be equipped with the insulating layer between the inner wall of box body, be equipped with temperature sensing color development piece on the insulating layer. The utility model is used for the sample that keeps when PCR spotting operation is in low temperature environment to can indicate operating personnel temperature variation, in time change the refrigerant, the sample is inefficacy not when guaranteeing the spotting operation.

Description

Novel PCR temperature control sample application box

The technical field is as follows:

the utility model relates to a medical science experiment technical field, concretely relates to novel PCR accuse temperature point appearance box.

Background art:

PCR (polymerase chain reaction) sample application is often required in PCR experiments, in order to ensure the stability of a detection sample of a detection reagent box in the experiment, low-temperature operation is required for reagent sample application and preservation in the existing experiment, and external pollution in the repeated operation process is also avoided. In addition, leakage or error point is easy to cause due to visual and mental fatigue in the repeated sample application process, the consumption time is long from the preparation of the detection reagent and the sample to be detected to the completion of sample application, and the reagent is easy to heat up and lose efficacy. Patent No. ZL201821937261 sets up the ice chest through the lower part at the lid, sets up the notch channel that can hold a plurality of centrifuging tubes on the lid, and the ice chest is all around with bottom cavity for put into the refrigerant, lower floor's ice chest can be inserted to the lid, with the low temperature effect of better maintenance device. But the sample preservation and the continuous sample application operation of sample after this kind of structure can't realize the point sample, and the sample after the point sample is accomplished can not deposit at low temperature and lead to losing efficacy, and operating personnel also can not audio-visually observe whether the temperature is suitable, and it is untimely to change the refrigerant easily, and refrigeration effect is not good to make the improper temperature lead to the point sample failure.

The utility model has the following contents:

technical problem to be solved

The utility model aims at providing a novel PCR accuse temperature point appearance box for the sample that keeps when PCR sample application is operated is in the low temperature environment, solves that the sample can not deposit at low temperature and lead to becoming invalid after the current equipment sample application is accomplished, and operating personnel also can not audio-visual observation temperature suitable, changes the refrigerant untimely easily, and refrigeration effect is not good, thereby makes the improper problem that leads to the sample application failure of temperature.

The technical scheme is as follows:

in order to solve the technical problem, the utility model adopts the following technical scheme: the utility model provides a novel PCR accuse temperature point appearance box, box body and lid including the transparent plastic preparation, the box body intussuseption is filled with the heat conduction material piece, heat conduction material piece top is equipped with a plurality of test-tube groove, the bottom of box body is equipped with the refrigerant groove, the refrigerant inslot is equipped with refrigerant box, refrigerant box is through setting up the opening of box body side is put into the refrigerant inslot, placed the refrigerant in the refrigerant box, the refrigerant with the contact of heat conduction material piece, the heat conduction material piece with be equipped with the insulating layer between the inner wall of box body, be equipped with the temperature sensing on the insulating layer and show the color lump.

The test tube groove is used for placing a sample reagent for PCR sample application and a reagent after sample application, the refrigerant box is placed in the refrigerant groove, the refrigerant is in contact with the heat-conducting material blocks to cool the sample in the test tube groove, the heat-insulating layer is used for slowing down the transmission of heat outside the box body to the heat-conducting material blocks so as to keep the low-temperature state in the test tube groove, and the temperature sensing color-displaying block is used for reminding an operator of timely replacing the refrigerant in the refrigerant box through color change when the temperature of the heat-conducting material blocks is too high so as to keep the low temperature of the test tube groove and prevent the sample from losing efficacy due to temperature rise.

Further, the box body is separated into three region by the baffle, lays the district for PCR point model respectively, has configured the sample and has deposited district and former state safety and put the district, the test tube slot distributes PCR point model lays the district, have configured the sample deposit the district with former sample is laid in the district, and the operating personnel of being convenient for carries out the deposit of sample application operation and sample application back sample that finishes to conveniently distinguish different kinds of reagent, realize article regional planning such as sample and reagent, guide the operator to carry out the accuracy and operate.

Furthermore, the heat conducting material block is made of heat conducting insulating elastic rubber, the heat conducting effect is good, the weight is light, the heat insulation layer is made of pearl wool, the heat insulation effect is good, the heat insulation layer is non-toxic, the refrigerant is ice cubes, and the ice cube refrigerating device is convenient to obtain when the refrigerating effect is good.

Further, the temperature sensing color development block is made of reversible temperature sensing color change materials, the color change temperature is 4-10 ℃, and the temperature required by PCR sample application cannot be higher than 10 ℃, so that when the temperature in the heat conduction material block is higher than 4-10 ℃, the temperature sensing color development block changes color, an operator is prompted to replace the refrigerant immediately, and the overhigh temperature is prevented.

Furthermore, a handle is arranged on one side, close to the opening, of the refrigerant box, and the handle can be conveniently placed into and drawn out of the refrigerant box by an operator, so that the refrigerator is convenient to use.

(III) the beneficial effects are as follows:

compared with the prior art, the utility model discloses the beneficial effect who produces is: through set up the heat conduction material piece in the box body, the below of box body sets up the refrigerant groove that can supply the refrigerant box to put into and take out, sets up the test tube groove in the heat conduction material piece, sets up insulating layer and temperature sensing color development piece between heat conduction material piece and the box body inner wall, can keep the low temperature state of PCR sample application operation in-process sample reagent, reminds operating personnel in time to change the refrigerant through discolouring of temperature sensing color development piece when the high temperature simultaneously, effectively avoids the sample inefficacy.

Description of the drawings:

in order to more clearly illustrate the technical solution in the embodiments of the present invention, the drawings required to be used in the embodiments will be briefly described below.

FIG. 1 is a top view of a novel PCR temperature-controlled spotting box according to an embodiment of the present invention;

FIG. 2 is a side view of a novel PCR temperature-controlled spot box according to an embodiment of the present invention;

FIG. 3 is a cross-sectional view of the novel PCR controlled temperature spot kit A-A according to the embodiment of the present invention;

in the figure: 1. a box body; 2. a box cover; 3. a block of thermally conductive material; 4. a test tube groove; 5. a refrigerant tank; 6. a refrigerant box; 7. an opening; 8. a refrigerant; 9. a thermal insulation layer; 10. the temperature sensing color display block; 11. a partition plate; 12. a PCR spotting plate placing region; 13. a configured sample storage area; 14. an original sample placement area; 15. handle bar

The specific implementation mode is as follows:

the technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.

As shown in figure 1, a novel PCR temperature control point appearance box that is shown in figure 2 and figure 3, box body 1 and lid 2 including the transparent plastic preparation, box body 1 intussuseption is filled with heat conduction material piece 3, heat conduction material piece 3 top is equipped with a plurality of test tube groove 4, the bottom of box body 1 is equipped with refrigerant groove 5, be equipped with refrigerant box 6 in the refrigerant groove 5, refrigerant box 6 places into refrigerant groove 5 through setting up opening 7 in box body 1 side, refrigerant 8 has been placed in the refrigerant box 6, refrigerant 8 and heat conduction material piece 3 contact, be equipped with insulating layer 9 between the inner wall of heat conduction material piece 3 and box body 1, be equipped with temperature sensing color lump 10 on insulating layer 9.

Preferably, the box body 1 is divided into three areas by the partition boards 11, namely a PCR sample placing area 12, a configured sample storing area 13 and an original sample placing area 14, the test tube slots 4 are distributed in the PCR sample placing area 12, the configured sample storing area 13 and the original sample placing area 14, so that an operator can conveniently perform sample application operation and store samples after sample application is finished, different types of reagents can be conveniently distinguished, regional planning of articles such as samples and reagents can be realized, and the operator can be guided to perform accurate operation.

Preferably, the heat conducting material block 3 is made of heat conducting insulating elastic rubber, the heat conducting effect is good, the weight is light, the heat insulation layer 9 is made of pearl wool, the heat insulation effect is good, the heat insulation layer is non-toxic, the refrigerant 8 is ice cubes, and the ice cube is good in refrigerating effect and convenient to obtain.

Preferably, the temperature-sensing color-developing block 10 is a reversible temperature-sensing color-changing material, the color-changing temperature is 4-10 ℃, and because the temperature required by PCR sample application cannot be higher than 10 ℃, when the temperature in the heat-conducting material block 3 is higher than 4-10 ℃, the temperature-sensing color-developing block 10 changes color, and prompts an operator to immediately replace the refrigerant 8 to prevent overhigh temperature.

Preferably, a handle 15 is provided on the side of the refrigerant box 6 near the opening 7, and the handle 15 is convenient for an operator to put and withdraw the refrigerant box 6 into and out of the refrigerant tank 5 for use.

The utility model discloses a novel PCR accuse temperature point appearance box's application method as follows:

the original sample to be spotted is placed in the test tube groove 4 in the original sample placement region 14, the sample placement operation is carried out in the test tube groove 4 in the PCR sample placement plate placement region 12, the sample after the sample placement is placed in the test tube groove 4 in the configured sample storage region 13, the refrigerant 8 is placed in the refrigerant box 6 and then inserted into the refrigerant groove 5 through the opening 7, the refrigerant 8 is contacted with the heat-conducting material block 3, the sample in the test tube groove 4 is cooled, the heat insulation layer 9 can slow down the heat outside the box body 1 from transferring to the heat conduction material block 3 so as to keep the low temperature state in the test tube groove 4, when the refrigerating capacity of the refrigerant 8 is insufficient and the heat conducting material block 3 is heated, the color of the temperature sensing color displaying block 10 is changed, and an operator can replace the refrigerant 8 in time according to the observed color change so as to keep the low temperature of the test tube groove 4 and prevent the sample from losing efficacy due to heating.

To sum up, the utility model provides a novel PCR accuse temperature point appearance box for keeping the sample when PCR spotting operation is in low temperature environment, solve present equipment spotting and accomplish the back sample and can not deposit at low temperature and lead to becoming invalid, and operating personnel also can not audio-visual observation temperature suitable, change the refrigerant untimely easily, refrigeration effect is not good, thereby makes the improper problem that leads to the spotting failure of temperature.

The present invention has been described above by way of example, but the present invention is not limited to the above-mentioned embodiments, and any modification or variation based on the present invention is within the scope of the present invention.

Claims (5)

1. The utility model provides a novel PCR accuse temperature point appearance box, includes box body and lid of transparent plastic preparation, its characterized in that: the box body is filled with heat-conducting material blocks, a plurality of test tube grooves are formed in the top of each heat-conducting material block, a refrigerant groove is formed in the bottom of the box body, a refrigerant box is arranged in the refrigerant groove, the refrigerant box is placed into the refrigerant groove through an opening formed in the side face of the box body, a refrigerant is placed in the refrigerant box and is in contact with the heat-conducting material blocks, a heat insulation layer is arranged between the heat-conducting material blocks and the inner wall of the box body, and temperature sensing color-displaying blocks are arranged on the heat insulation layer.

2. The novel PCR controlled temperature sample application kit according to claim 1, wherein: the box body is divided into three areas by partition plates, namely a PCR sample plate placing area, a configured sample storing area and an original sample intrinsic placing area, the test tube grooves are distributed in the PCR sample plate placing area, the configured sample storing area and the original sample placing area.

3. The novel PCR controlled temperature sample application kit according to claim 1, wherein: the heat conducting material block is made of heat conducting insulating elastic rubber, the heat insulation layer is made of pearl wool, and the refrigerant is ice blocks.

4. The novel PCR controlled temperature sample application kit according to claim 1, wherein: the temperature-sensing color-developing block is a reversible temperature-sensing color-changing material, and the color-changing temperature is 4-10 ℃.

5. The novel PCR controlled temperature sample application kit according to claim 1, wherein: and a handle is arranged on one side of the refrigerant box close to the opening.

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