CN211881811U

CN211881811U - External culture apparatus of cyst cercaria

Info

Publication number: CN211881811U
Application number: CN202020286868.3U
Authority: CN
Inventors: 付永; 沈秀英
Original assignee: Qinghai Academy of Animal Science and Veterinary Medicine
Current assignee: Qinghai Academy of Animal Science and Veterinary Medicine
Priority date: 2020-03-10
Filing date: 2020-03-10
Publication date: 2020-11-10
Anticipated expiration: 2030-03-10

Abstract

The utility model discloses an external culture apparatus of cyst cercaria, include: the double-layer bracket of the culture bottle comprises four vertical supporting upright rods, two first horizontal supporting rods and two second horizontal supporting rods; the first horizontal supporting rod is detachably connected with the vertical supporting upright rod; the culture bottle comprises a culture bottle body, and a sample adding port and a filtering port which are respectively arranged at two ends of the culture bottle body; the sample adding port is inclined upwards along the horizontal extension line of the culture bottle body, and the filtering port is inclined downwards along the horizontal extension line of the culture bottle body; a filtering membrane is arranged inside the filtering port; the sample adding port and the filtering port are integrally buckled and sealed by a ventilating cover; when two culture bottles are placed on the culture bottle double-layer support, the filtering port of the culture bottle positioned on the upper layer can be just aligned to the sample adding port of the culture bottle positioned on the lower layer. The utility model discloses not only improve the survival rate of soaking the cercaria, improved soaking the cercaria greatly moreover choose speed and efficiency, had stronger practicality.

Description

External culture apparatus of cyst cercaria

Technical Field

The utility model relates to a biological equipment field, more specifically the saying so relates to an external culture apparatus of cyst cercaria.

Background

The alveolar echinococcosis is a kind of zoonosis caused by the larva cyst metacercaria of echinococcus multilocularis. The alveolar echinococcosis is called 'worm cancer', and becomes one of chronic infectious diseases with the highest human mortality, particularly, the alveolar echinococcosis is a rare high-incidence epidemic area of alveolar echinococcosis in the source region of the three rivers in Qinghai, and the incidence rate of people in remote pastoral areas is up to 10%. However, the slow development of the pathogenic alveolar miracidium and the limited sample sources limit the deep research on the pathogenic mechanism of the alveolar echinococcosis.

The in vitro culture of the oncosphere larvae can solve the problem that the sample source is difficult in the current oncosphere research, but the traditional cell culture dish is not easy to pick out large vesicles formed in the oncosphere development process and dead oncosphere, so that the picking rate of the oncosphere is too low.

Therefore, a specific device for culturing the oncosphere larvae is developed, which not only can realize the in-vitro culture of the oncosphere larvae and improve the survival rate of the oncosphere larvae, but also can effectively improve the picking speed and efficiency of the oncosphere larvae, and is a direction for the current needs of technical personnel in the field to focus on research.

SUMMERY OF THE UTILITY MODEL

Therefore, the utility model aims at providing an external culture apparatus of oncosphere larva, its specific technical scheme as follows:

an in-vitro culture device for oncosphere larvae comprises:

the double-layer bracket of the culture bottle comprises four vertical supporting vertical rods which are parallel to each other, two first horizontal supporting rods which are arranged at the same position below the vertical supporting vertical rods and are parallel to each other, and two second horizontal supporting rods which are fixed at the tops of the vertical supporting vertical rods and are parallel to each other; the first horizontal supporting rod is detachably connected with the vertical supporting vertical rods so as to realize that the vertical height of the first horizontal supporting rod between the vertical supporting vertical rods is adjustable;

the culture bottle comprises a culture bottle body and bottle openings which are arranged at two ends of the culture bottle body and are integrally connected with the culture bottle body; the two bottle openings are respectively a sample adding opening and a filtering opening; the sample adding port is inclined upwards along the horizontal extension line of the culture bottle body, and the filtering port is inclined downwards along the horizontal extension line of the culture bottle body; the bottle mouth end part of the sample adding port is externally expanded into a horn shape, and a filtering membrane is arranged inside the filtering port; the sample adding port and the filtering port are integrally buckled and sealed by a ventilating cover;

when the two culture bottles are placed on the culture bottle double-layer support, the filtering port of the culture bottle positioned on the upper layer can be just aligned to the sample adding port of the culture bottle positioned on the lower layer.

Through adopting the technical scheme, the utility model provides a special external culture apparatus to steep ball worm, the device wholly comprises blake bottle double-deck support and two blake bottles on arranging the blake bottle double-deck support in, and it can carry out the external cultivation of bubble ball worm and twice filtration after realizing the cultivation of bubble ball worm culture solution, has not only improved the survival rate of steeping ball worm greatly, and the bulk operation is got up conveniently, swiftly moreover, has improved the speed and the efficiency of choosing of steeping ball worm greatly.

On the basis of the technical scheme, the utility model discloses still can make following improvement:

preferably, a plurality of threaded holes are formed in the four vertical supporting vertical rods along the axial direction of the vertical supporting vertical rods at intervals, and the first horizontal supporting rod is fixedly connected with the vertical supporting vertical rods through adjusting knobs matched with the threaded holes.

According to the technical scheme, the first horizontal supporting rod can be vertically adjusted on the vertical supporting upright rod through the screwing and fixing of the adjusting knob and the threaded holes with different heights.

Preferably, a groove capable of supporting the culture bottle sample adding port and the filter port is designed in the middle of or close to the middle of the two second horizontal support rods.

The culture bottle on the upper layer has relatively safe and stable placing space due to the arrangement of the groove, and the condition that the culture bottle falls off due to the shaking of the support or the accidental hanging collision of an operator is avoided.

Preferably, the depth of the groove is determined by that the top surface of the bottle body of the culture bottle is not higher than the top surface of the vertical supporting upright rod, so that the culture bottle on the upper layer is prevented from being hung down.

Preferably, the included angle between the sample adding port, the filtering port and the horizontal extension line of the culture bottle body ranges from 40 degrees to 50 degrees.

Preferably, the aperture of the sample adding port is larger than that of the filtering port, so as to further ensure that the sample adding port of the lower layer culture bottle can receive all culture solution flowing out of the filtering port of the upper layer culture bottle.

Preferably, the flask body of the culture flask is correspondingly marked with capacity scale marks.

Preferably, the membrane aperture of the filter membrane is 1mm, so that larger vesicles formed in the development process of the alveolar miracidium and dead alveolar miracidium are effectively filtered.

Preferably, the material of blake bottle is polystyrene, and polystyrene has excellent optical property, is convenient for microscope observation.

Preferably, the double-layer bracket of the culture bottle is made of P steel with high strength level.

The utility model relates to an external culture apparatus of bubble cercaria has both realized the external culture of bubble cercaria, can realize rapid filtration to bubble cercaria culture solution again, has not only improved bubble cercaria survival rate, has still improved the speed and the efficiency of choosing of bubble cercaria, and the practicality is strong, is fit for popularizing and applying.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.

FIG. 1 is a schematic view of the overall installation structure of the external culture device for the cyst larvae of the present invention.

FIG. 2 is a schematic view of the double-layered support of the culture bottle in the device of the present invention.

FIG. 3 is a schematic view of the structure of the culture flask of the device of the present invention.

Wherein, in the figure,

100-a double-layer bracket of a culture bottle,

101-vertical supporting upright rod, 102-first horizontal supporting rod, 103-second horizontal supporting rod, 104-threaded hole, 105-adjusting knob and 106-groove;

200-a culture flask, wherein the culture flask is a hollow flask,

201-flask body, 202-sample port, 203-filtration port, 204-filtration membrane, 205-air-permeable cover, 206-volume scale mark.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary and intended to be used for explaining the present invention, and should not be construed as limiting the present invention.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In the present disclosure, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may comprise direct contact between the first and second features, or may comprise contact between the first and second features not directly. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.

Example (b):

an in vitro culture device for oncosphere larvae according to the embodiment of the present invention is described in detail with reference to FIGS. 1-3.

As shown in figure 1, the embodiment of the utility model discloses an external culture device of oncosphere larva, include: a culture bottle double-layer bracket 100 and a culture bottle 200.

In particular, the method comprises the following steps of,

as shown in fig. 2, in the present embodiment, the double-layer support 100 for the culture bottle is made of P-steel, and includes four vertical supporting vertical rods 101 parallel to each other, two first horizontal supporting rods 102 installed at the same position below the vertical supporting vertical rods 101 and parallel to each other, and two second horizontal supporting rods 103 fixed on the top of the vertical supporting vertical rods 101 and parallel to each other.

The first horizontal support rod 102 is detachably connected with the vertical support upright rods 101, so that the vertical height of the first horizontal support rod 102 between the vertical support upright rods 101 can be adjusted.

Specifically, in this embodiment, five threaded holes 104 are formed in the four vertical supporting vertical rods 101 at intervals along the axial direction of the vertical supporting vertical rods 101, and the first horizontal supporting rod 102 is fixedly connected with the vertical supporting vertical rods 101 through an adjusting knob 105 adapted to the threaded holes 104. That is, the screwing and fixing of the adjusting knob 105 and the threaded holes 104 with different heights enables the vertical height of the first horizontal supporting rod 102 on the vertical supporting upright 101 to be adjusted.

As shown in fig. 3, in the present embodiment, the material of the culture bottle 200 is polystyrene, and the culture bottle includes a bottle body 201 and bottle openings disposed at two ends of the bottle body 201 and integrally connected thereto; wherein, the two bottle mouths are respectively a sample adding mouth 202 and a filtering mouth 203.

The sample adding port 202 is inclined upwards along the horizontal extension line of the culture bottle body 201, and the filtering port 203 is inclined downwards along the horizontal extension line of the culture bottle body 201; the bottle mouth end of the sample adding port 202 is externally expanded into a horn shape, and a filtering membrane 204 is arranged inside the filtering port 203; the sample addition port 202 and the filtration port 203 are integrally closed by a gas-permeable cover 205.

In this embodiment, when two culture bottles 200 are placed together on the double-layered rack 100, the filtration port 203 of the culture bottle 200 located on the upper layer can be aligned with the sample addition port 202 of the culture bottle 200 located on the lower layer.

Furthermore, the middle or the position near the middle of the two second horizontal supporting rods 103 is provided with a groove 106 capable of supporting the sample adding port 202 and the filtering port 203 of the culture bottle, and the depth of the groove 106 is determined based on the fact that the top surface of the culture bottle body 201 is not higher than the top surface of the vertical supporting upright rod 101, so that the culture bottle 200 positioned at the top is not easy to drop.

Furthermore, the included angle between the sample addition port 202 and the filtration port 203 and the horizontal extension line of the flask body 201 is 40-50 degrees, and more preferably 40 degrees.

Furthermore, the aperture of the sample addition port 202 is generally larger than that of the filtration port 203, so that the culture solution can be injected and can be completely received by the culture solution flowing downwards from the upper part during one filtration.

Furthermore, the flask body 201 is correspondingly marked with a volume scale line 206.

Further, the membrane aperture of the filtration membrane 204 is 1 mm.

When the utility model is used, firstly, a specified amount of culture solution with pathogenic cyst larvae (accurately judged according to the capacity scale line 206 on the culture bottle 200) is put in one of the culture bottles 200 in advance, and then the culture solution is put on the upper layer of the culture bottle double-layer bracket 100, namely the second horizontal support rod 103; another culture bottle 200 is placed on the lower layer of the culture bottle double-layer bracket 100, namely the first horizontal support rod 102, and the sample adding port 202 of the culture bottle 200 positioned on the lower layer can be just aligned to the filtering port 203 of the upper layer culture bottle 200 by adjusting the height of the first horizontal support rod 102, so that the effect that the sample adding port 202 of the lower layer culture bottle 200 can receive all the culture solution flowing out from the filtering port 203 of the upper layer culture bottle 200 is achieved.

After the culture solution for the cyst larvae in the upper culture bottle 200 reaches the sufficient culture time, the operator can simultaneously open the filter port 203 on the upper culture bottle 200 and the sample port 202 on the lower culture bottle 200, so that the culture solution with the pathogenic cyst larvae just flows into the lower culture bottle 200 after being filtered by the filter membrane 204, and the large vesicles formed in the first culture process and the dead cyst larvae are blocked in the upper culture bottle 200; the culture solution with the pathogenic cyst larvae is continuously cultured for the second time in the lower layer culture bottle 200, after the culture solution of the cyst larvae in the lower layer culture bottle 200 reaches enough culture time, the culture solution flows out from the filtering port 203 of the lower layer culture bottle 200, larger vesicles formed in the second culture process and dead cyst larvae are filtered out again, and finally the pure culture solution of the living cyst larvae is obtained.

The utility model discloses simple structure, the practicality is good, can carry out the external culture of bubble cercaria and the filtration of twice bubble cercaria culture solution, has not only improved bubble cercaria survival rate, and whole process operation is got up conveniently, swiftly moreover, has improved the speed and the efficiency of choosing of bubble cercaria greatly.

The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. An external culture device for the cyst larvae is characterized by comprising:

the culture bottle double-layer support (100) comprises four vertical supporting vertical rods (101) which are parallel to each other, two first horizontal supporting rods (102) which are arranged at the same position below the vertical supporting vertical rods (101) and are parallel to each other, and two second horizontal supporting rods (103) which are fixed at the tops of the vertical supporting vertical rods (101) and are parallel to each other; the first horizontal supporting rod (102) is detachably connected with the vertical supporting vertical rods (101) so as to realize that the vertical height of the first horizontal supporting rod (102) between the vertical supporting vertical rods (101) is adjustable;

the culture bottle (200) comprises a culture bottle body (201) and bottle openings which are arranged at two ends of the culture bottle body (201) and integrally connected with the culture bottle body (200); the two bottle mouths are respectively a sample adding mouth (202) and a filtering mouth (203); the sample adding port (202) is inclined upwards along the horizontal extension line of the culture bottle body (201), and the filtering port (203) is inclined downwards along the horizontal extension line of the culture bottle body (201); the bottle mouth end part of the sample adding port (202) is externally expanded into a horn shape, and a filtering membrane (204) is arranged inside the filtering port (203); the sample adding port (202) and the filtering port (203) are integrally buckled and closed by a ventilation cover (205);

when two culture bottles (200) are placed on the culture bottle double-layer bracket (100), the filtering opening (203) of the culture bottle (200) positioned at the upper layer can be just aligned with the sample adding opening (202) of the culture bottle (200) positioned at the lower layer.

2. The device for in-vitro culture of the oncosphere larvae according to claim 1, wherein a plurality of threaded holes (104) are formed in each of the four vertical supporting vertical rods (101) at intervals along the axial direction of the vertical supporting vertical rods, and the first horizontal supporting rod (102) is fixedly connected with the vertical supporting vertical rods (101) through an adjusting knob (105) matched with the threaded holes (104).

3. The device for in vitro culture of the oncosphere larvae according to claim 1, wherein the middle or the position near the middle of the two second horizontal support bars (103) is provided with a groove (106) capable of supporting the sample addition port (202) and the filtration port (203) of the culture bottle.

4. The device for the in vitro culture of the oncosphere larvae according to claim 3, wherein the depth of the grooves (106) is determined so that the top surface of the flask body (201) is not higher than the top surface of the vertical supporting upright (101).

5. The in vitro culture device for the oncosphere larvae according to claim 1, wherein the included angle between the sample adding port (202), the filtering port (203) and the horizontal extension line of the culture bottle body (201) is 40-50 degrees.

6. The device for in vitro culture of the oncosphere larvae according to claim 1, wherein the aperture of the sample addition port (202) is larger than the aperture of the filter port (203).

7. The device for in-vitro culture of the oncosphere larvae according to claim 1, wherein the flask body (201) of the culture flask is correspondingly marked with volume scale marks (206).

8. The device for the in vitro culture of the oncosphere larvae according to the claim 1, wherein the membrane aperture of the filter membrane (204) is 1 mm.

9. The device for in vitro culture of the oncosphere larvae according to claim 1, wherein the culture bottle (200) is made of polystyrene.

10. The device for in vitro culture of the oncosphere larvae according to claim 1, wherein the double-layer bracket (100) of the culture bottle is made of P steel.