CN211861494U - Device for collecting and counting cryptocaryon irritans cysts - Google Patents
Device for collecting and counting cryptocaryon irritans cysts Download PDFInfo
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- CN211861494U CN211861494U CN201922029148.9U CN201922029148U CN211861494U CN 211861494 U CN211861494 U CN 211861494U CN 201922029148 U CN201922029148 U CN 201922029148U CN 211861494 U CN211861494 U CN 211861494U
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The utility model provides a device for collecting and counting cryptocaryon irritans cyst. The device comprises a collection cloth liner 1, an open container 2, a fence 3 and a ventilation device 4, wherein the collection cloth liner 1 is laid at the bottom of the open container 2, the fence 3 which is communicated up and down is placed on the collection cloth liner 1, and the bottom area of the fence 3 is smaller than that of the collection cloth liner 1; the aeration means 4 is suspended from the middle of the middle end of the enclosure 3. Device simple structure, application method is simple, the whole cysts that drop on the collection fish body that can be accurate, then accurate count, can calculate the infection rate that obtains amazing cryptocaryon insect, the anti amazing cryptocaryon insect's of aassessment fish ability size that can be accurate, simple and convenient.
Description
Technical Field
The utility model belongs to the technical field of fish parasite control. And more particularly, to a device for collecting and counting cryptocaryon irritans cysts.
Background
Cryptocaryon irritans is a parasitic disease caused by cryptocaryon irritans parasitizing on the body surface of marine teleostean fish, and a large number of macroscopic 'small white spots' can be formed on the body surfaces of skin, fin rays, gills and the like of the fish infected with the parasite, and the disease is also called 'white spot disease'. The cryptocaryon irritans can almost infect all cultivated marine teleostean fishes in south China, is the parasitic disease with the most serious harm at present, and can cause hundreds of millions of economic losses every year. In recent years, a large number of research works on cryptocaryon irritans prevention and treatment including immune prevention and treatment, drug prevention and treatment, ecological prevention and treatment and the like are carried out by scholars at home and abroad. The evaluation of the prevention and control effect is a key link for the prevention and control research of cryptocaryon irritans, wherein the infection rate of cryptocaryon irritans to fish is a main index for evaluating the insect resistance of fish bodies.
The life history of cryptocaryon irritans includes 4 stages of trophozoites, cysts precursors, cysts and larvae, wherein trophozoites are the stages of parasitizing fish bodies, cysts precursors and cysts are the stages of division and reproduction, and larvae are the stages of infection. The intensity of infection of fish by cryptocaryon irritans is currently evaluated mainly against the parasitic stages of the parasite: the infection intensity of the fish is represented by the ratio of the number of trophozoites of the second gill arch on the right side of the fish to the body weight of the fish. Although the method is short in time consumption and simple, the method causes large error of results due to large difference between fish bodies. In addition, this method does not allow assessment of the rate of infection of fish by cryptocaryon irritans. Therefore, a more scientific and accurate method for evaluating the infection intensity of the cryptocaryon irritans in the fish body needs to be found.
Comparison of the infection rate of cryptocaryon irritans is the most direct and accurate indicator for evaluating the cryptocaryon irritans resistance of fish, however, the total trophozoite number of fish is difficult to be counted. During the process from larva infection to cyst formation, a larva invading the fish body develops and matures through trophozoite, and finally an cyst is formed. Therefore, the capsule which is completely dropped by the collected fish body can directly respond to the infection rate of the cryptocaryon irritans to the fish, but the capsule which is directly collected at the bottom of the fish pond has large workload, large quantity of lost capsules and broken parts of capsules, and finally the infection rate is calculated inaccurately. It is therefore desirable to provide a device for accurately collecting and counting capsules.
Disclosure of Invention
The utility model aims at providing a device for collecting and count amazing cryptocaryon irritans cyst. The device can be accurate the whole cysts that drop on the collection fish body, then after the accurate count, can calculate the infection rate that obtains amazing cryptocaryon insect, the anti-amazing cryptocaryon insect's of aassessment fish ability size that can be accurate, simple and convenient.
A device for collecting and counting cryptocaryon irritans cysts comprises a collecting cloth liner, an open container, a fence and a ventilation device, wherein the collecting cloth liner is laid at the bottom of the open container, the fence which is communicated up and down is placed on the collecting cloth liner, and the area of the bottom of the fence is smaller than that of the collecting cloth liner; the ventilating device is suspended at the middle position of the middle end of the fence.
The device for collecting and counting cryptocaryon irritans cysts is used by the following method:
adding water for cultivation into an open container, placing a fence on a collecting cloth liner, then placing the fish infected with cryptocaryon irritans into water, then placing the fish into a ventilation device, cultivating the fish in still water, then replacing the collecting cloth liner with new ones every day, and counting the number of cysts on the collecting cloth liner, wherein the growth and development cycles of the trophozoites of cryptocaryon irritans at different temperatures are different, and the time for the trophozoites to develop on the fish body to cysts is about 2-12d under the condition of 18-32 ℃, so that the cysts which completely fall off on the fish body can be collected after the trophozoites are continuously used for collecting and counting for 2-12 d. Different collection strategies are set for different fishes at different water temperatures.
And then calculating the total number of cysts, and calculating the infection rate of the cryptocaryon irritans according to the total number of cysts collected and/or the infection dose of 100, thereby accurately evaluating the capacity of the fish to resist the cryptocaryon irritans.
Preferably, the collecting cloth liner is dark polyester rain awning cloth. The cyst is white, so that the dark collection cloth liner is selected, and the counting is easy and accurate; and the terylene awning cloth is provided with certain lines, the capsule is easy to adhere to the terylene awning cloth, and even if water with certain speed flows, the capsule can also adhere to the terylene awning cloth and is not easy to fall off, thereby ensuring the collection accuracy.
Preferably, the container and the pen may each be a barrel container.
Preferably, the pen is tapered with the upper end being smaller in diameter than the lower end. In order to ensure that the capsule falling off from the fish body cannot be adhered to the side wall of the fence, the fence is arranged to be in a conical shape, the capsule can be ensured to be completely adhered to the collecting cloth liner, and errors in the collecting process are reduced.
Preferably, the inner wall of the enclosure is a smooth inner wall.
Preferably, the enclosure is smaller in diameter and height than the container.
Preferably, the edge of the collection sheet is higher than the bottom end of the rail.
Because both the container and the pen have a certain weight, even if the pen is placed on the collection cloth liner only, the cysts are difficult to run off from the gap between the pen and the collection cloth liner in the process of still water cultivation. However, to ensure a complete solution, the edge of the collection pad can be raised too high above the bottom of the pen, but not so high as to lift the pen off the bottom of the container.
Preferably, a detachable hanging rod is further arranged in the middle of the top end of the container.
Preferably, the edge of the collecting cloth liner may be provided with an edge ring, which may be hung from a hanging rod.
Preferably, the ventilation device comprises a gas stone and a ventilation pipe which are communicated with each other, and the gas stone is hung on the hanging rod through the ventilation pipe.
Preferably, the air stone is suspended in the middle of the middle end of the fence. The middle position of the air stone hanging on the upper end of the fence is used for ensuring that the cysts falling off from the fish body can be uniformly distributed on the collecting laying cloth.
The utility model discloses following beneficial effect has:
device simple structure, application method is simple, the whole cysts that drop on the collection fish body that can be accurate, then after the accurate count, can calculate the infection rate that obtains amazing cryptocaryon insect, the anti amazing cryptocaryon insect's of aassessment fish ability size that can be accurate, simple and convenient.
Drawings
FIG. 1 is a schematic view showing the structure of the Cryptocaryon irritans-collecting encapsulation device of example 1.
FIG. 2 is a schematic view showing the structure of the Cryptocaryon irritans-collecting encapsulation device of example 1.
Fig. 3 is a schematic diagram showing different positions of the placement of the air stones.
Detailed Description
The present invention will be further described with reference to the following drawings and specific examples, which are not intended to limit the invention in any way. Unless otherwise indicated, the reagents, methods and apparatus employed in the present invention are conventional in the art.
Unless otherwise indicated, reagents and materials used in the present invention are commercially available.
Example 1
A device for collecting and counting cryptocaryon irritans cysts is shown in figure 1, and comprises a collecting cloth mat 1, an open container 2, a fence 3 and a ventilation device 4, wherein the collecting cloth mat 1 is laid at the bottom of the open container 2, the fence 3 which is communicated up and down is placed on the collecting cloth mat 1, and the bottom area of the fence 3 is smaller than that of the collecting cloth mat 1; the aeration means 4 is suspended in the middle of the middle end of the enclosure 3.
The use method of the device for collecting the cryptocaryon irritans comprises the following steps:
the water for breeding is added into the open container 2, the fence 3 is placed on the collecting cloth liner 1, then the fish infected with cryptocaryon irritans is placed into the water, then the ventilating device 4 is placed, the breeding is carried out in still water, then the collecting cloth liner 1 is replaced with new one every day, the number of cysts on the collecting cloth liner 1 is counted, and after 2 d-12 d is counted continuously, all cysts falling off from the fish body can be collected. Because the growth cycles of the cryptocaryon irritans at different temperatures are different, the cryptocaryon irritans can completely enter the cyst stage within 2-12 days in most cases, so that all the infected cryptocaryon irritans on fish bodies can be accurately collected.
And then calculating the total number of cysts, and calculating the infection rate of the cryptocaryon irritans according to the total number of cysts collected and/or the infection dose of 100, thereby accurately evaluating the capacity of the fish to resist the cryptocaryon irritans.
The collecting cloth liner 1 is deep color polyester rain awning cloth. The cyst is white, so that the dark collection cloth liner is selected, and the counting is easy and accurate; and the terylene awning cloth is provided with certain lines, the capsule is easy to adhere to the terylene awning cloth, and even if water with certain speed flows, the capsule can also adhere to the terylene awning cloth and is not easy to fall off, thereby ensuring the collection accuracy.
The container 2 and the enclosure 3 may each be a tub-shaped container.
The rail 3 is tapered with the upper end being smaller in diameter than the lower end. In order to ensure that the cysts falling off from the fish body cannot be adhered to the side wall of the fence 3, the fence 3 is arranged into a conical shape, so that the cysts can be completely adhered to the collecting cloth liner 1, and the error in the collecting process is reduced.
The inner wall of the fence 3 is a smooth inner wall.
The pen 3 is smaller in diameter and height than the container.
The edge of the collecting cloth mat 1 is higher than the bottom end of the fence 3.
Since the container 2 and the pen 3 each have a certain weight, even if the pen 3 is simply placed on the collection cloth 1, the cysts are difficult to run off from the gap between the pen 3 and the collection cloth 1 during the still water cultivation. However, to ensure a perfect fit, the edge of the collection pad 1 can be too high above the bottom end of the rail 3, but not so high as to lift the rail 3 off the bottom of the container.
The middle of the top end of the container 2 is also provided with a detachable hanging rod 21.
As shown in fig. 2, the edge of the collecting cloth liner 1 may be provided with an edge ring 11, and the edge ring 11 may be hung on a hanging bar 21.
The ventilating device 4 comprises a gas stone 41 and a ventilating pipe 42 which are communicated with each other, and the gas stone 41 is hung on the hanging rod 21 through the ventilating pipe 42.
The air stone 41 is hung in the upper end of the fence 3 at an intermediate position. The air stone 41 is hung in the middle of the middle end of the fence 3, so that the cysts falling off from the fish body can be uniformly distributed on the collecting cloth liner, and errors in the counting process are avoided.
When the hanging position of the air stone 41 is different, the hanging position is as shown in fig. 3, wherein fig. 3a shows that the air stone is positioned in the middle of the fence 3 and does not touch the bottom; fig. 3b shows the air stone in the middle of the fence 3, touching the bottom; fig. 3c shows the air stone on the contact wall of the fence 3, without touching the bottom; fig. 3d shows the air stone on the wall, bottom of the enclosure 3.
In addition, two air stones are respectively arranged, fig. 3e shows that the air stones are positioned on the contact wall of the fence 3, and the two air stones are positioned on one diameter of the fence 3; three air stones are respectively arranged, and the 3 air stones are positioned on the contact wall of the fence 3 and on the same horizontal plane to form an equilateral triangle.
The 6 groups, when the quantity and the position of the air stone are different, the distribution of the capsules on the collecting pad cloth 1 is different, and the result of the position collection of fig. 3a is that: the cysts are uniformly distributed on the collecting laying cloth 1;
the results collected for the FIG. 3b position are: the capsules are distributed in disorder, and the accumulation condition of partial positions appears, so that accurate counting is difficult;
the results collected for the fig. 3c positions are: the side close to the air stone 4 is provided with a few capsules which are distributed, and the other side is provided with a plurality of capsules which are stacked, so that accurate counting is difficult;
the results collected for the fig. 3d positions are: the capsules are distributed on one side close to the air stone 4, while the capsules on the other side are distributed in disorder, so that the accumulation of partial positions occurs, and accurate counting is difficult;
the positions shown in fig. 3e and 3f are not easy to be counted because of the random distribution of the capsules on the collecting cloth liner due to the plurality of air stones 4, and the accumulation of partial positions occurs.
According to the above, the air stone 4 is hung at the middle position of the middle end of the fence 3, and the capsules can be uniformly distributed on the collecting cloth mat 1, so that accurate counting is facilitated.
Example 2
The apparatus for collecting and counting cryptocaryon irritans cysts (as shown in fig. 1) in example 1 was used as a collecting apparatus, and the cryptocaryon irritans cysts were collected and the infection rate of cryptocaryon irritans was calculated.
After the device was prepared, the test was performed according to the following procedure:
artificial infection animal model test
An indoor simulation experiment is carried out by taking trachinotus ovatus as an infected object, and the specific process is as follows:
(1) experimental trachinotus ovatus temporary culture
Temporarily culturing 10-20 g of 30-trachinotus ovatus in each culture barrel at the water temperature of 30 +/-1 ℃, continuously aerating and culturing in running water. Feeding according to 6% of fish weight every day, 1.5% every time, and 4 times a day (7: 30, 11: 30, 15:30, 18: 30). The bottom of the breeding barrel is brushed once every 3 days, so that the cryptocaryon irritans infection is avoided.
(2) Cryptocaryon irritans infection test
Larvae incubated for 2h were used to infect trachinotus ovatus at doses of 2000, 1000, 500, 250 and 0 larvae/tail, with a water volume of 100L. After 2h of still water infection, transferring the trachinotus ovatus to a device without larvae for collecting and counting cryptocaryon irritans cysts, and carrying out running water temporary rearing according to the conditions in the step (1); then, at 2d, 3d and 4d nights after infection, the collection pad 1 was cultured in still water and replaced with a new one, and the number of cysts on the replaced pad was counted. Each set of experiments was in triplicate.
(3) Data statistics
The number of shedding capsules in each group was counted and subjected to linear analysis using R2And (3) evaluating the fitting degree of the curve, and evaluating whether the trachinotus ovatus infected by the larvae/tail dose of 0-2000 can accurately reflect the degree of the trachinotus ovatus infected by the cryptocaryon irritans under the method.
The statistical and calculation results are shown in table 1.
TABLE 1 encapsulation by collection of the pad at different infectious doses
| Infectious dose (larva/tail) | 2000 1000 | 500 | 250 | 0 | |
| Total number of capsules/ | 8209.67±137.44 | 3772±157.79 | 2462.33±339.17 | 1373±96.17 | 0 |
| Infection rate/%) | 13.68±0.23 12 | 57±0.53 | 16.42±2.26 18 | .31±1.28 | 0 |
| Average number of cysts per fish/fish | 273.66±4.58 12 | 5.73±5.26 | 82.08±11.31 | 45.77±3.21 | 0 |
From the above results, it was found that the number of capsules collected in each test group and the dose of infection showed a good linear relationship, and the linear equation was that y is 192.5+3.9612x, R20.990; at the same time, there is also a good linear relationship between the number of cysts collected per fish on average and the infectious dose, with the linear equation y being 6.41667+0.13204x, R2＝0.990。
The results show that the infection rate can be calculated by collecting cryptocaryon irritans cysts, when the infection dosage is 0-2000 larvae/tails, the total number of the cysts which fall off and the average number of cysts generated by each fish have a good linear relation with the infection dosage, the infection rate of cryptocaryon irritans on trachinotus ovatus can be accurately calculated, and the infection degree of cryptocaryon irritans on fish bodies can be effectively evaluated.
It should be finally noted that the above embodiments are only intended to illustrate the technical solutions of the present invention, and not to limit the scope of the present invention, and that those skilled in the art can make variations or modifications in other different forms based on the above description and thought, and all embodiments need not be exhaustive. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.
Claims (10)
1. The device for collecting and counting cryptocaryon irritans cysts is characterized by comprising a collecting cloth mat (1), an open container (2), a fence (3) and a ventilating device (4), wherein the collecting cloth mat (1) is laid at the bottom of the open container (2), the fence (3) which is communicated up and down is placed on the collecting cloth mat (1), and the area of the bottom of the fence (3) is smaller than that of the collecting cloth mat (1); the ventilating device (4) is suspended at the middle position of the middle end of the fence (3).
2. Device for collecting and counting cryptocaryon irritans cysts according to claim 1, characterized in that the collecting mat (1) is a dark polyester rainfly.
3. Device for collecting and counting cryptocaryon irritans cysts according to claim 1, characterized in that the container (2) and the pen (3) can both be tubbined containers.
4. Device for collecting and counting Cryptocaryon irritans cysts according to claim 3, characterized in that the pen (3) is tapered with an upper end smaller in diameter than a lower end.
5. Device for collecting and counting Cryptocaryon irritans cysts according to claim 3, characterized in that the inner walls of the pen (3) are smooth inner walls.
6. Device for collecting and counting cryptocaryon irritans cysts according to claim 3, characterized in that the pen (3) is smaller in diameter and height than the container (2).
7. Device for collecting and counting cryptocaryon irritans cysts according to claim 1, characterized in that the edges of the collecting cloth mat (1) are higher than the bottom end of the pen (3).
8. Device for collecting and counting cryptocaryon irritans capsules according to claim 1, characterized in that the container (2) is also provided with a removable hanging bar (21) in the middle of its top end.
9. Device for collecting and counting cryptocaryon irritans cysts according to claim 8, characterized in that the aeration means (4) comprise an air stone (41) and an aeration pipe (42) communicating with each other, the air stone (41) being suspended on the hanging bar (21) by means of the aeration pipe (42).
10. Device for collecting and counting cryptocaryon irritans cysts according to claim 9, characterized in that the air stones (41) are suspended in the middle of the middle end of the pen (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922029148.9U CN211861494U (en) | 2019-11-20 | 2019-11-20 | Device for collecting and counting cryptocaryon irritans cysts |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922029148.9U CN211861494U (en) | 2019-11-20 | 2019-11-20 | Device for collecting and counting cryptocaryon irritans cysts |
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| CN211861494U true CN211861494U (en) | 2020-11-06 |
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| CN201922029148.9U Active CN211861494U (en) | 2019-11-20 | 2019-11-20 | Device for collecting and counting cryptocaryon irritans cysts |
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