CN211848003U

CN211848003U - Ultrasonic cell harvesting device

Info

Publication number: CN211848003U
Application number: CN202020050496.4U
Authority: CN
Inventors: 罗火生; 马颖; 罗成; 周栋; 罗鸣
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-03-24
Filing date: 2020-01-10
Publication date: 2020-11-03
Anticipated expiration: 2030-01-10

Abstract

The utility model discloses an ultrasonic wave results cell device, it includes ultrasonic device and cell factory, and ultrasonic device's vibration end is placed in the cell factory outside, through producing the vibration that the ultrasonic wave realized the cell factory, and then peels off the cell from the cell factory wall, and the in-process is through horizontal migration device and vertical mobile device control vibration end relative cell factory horizontal migration and vertical removal, accomplishes the peeling off to the different layers of cell factory. The method has the advantages of simple process, short time consumption, high efficiency, less virus loss and improvement of virus yield, and reduces pollution risk; in addition, partial cells can be simultaneously crushed in the ultrasonic harvesting process, the pressure of the subsequent crushing step is reduced, and the ultrasonic cell disruption machine is particularly suitable for a cell culture tool for batch production in a cell factory.

Description

Ultrasonic cell harvesting device

Technical Field

The invention relates to the technical field of biological products, in particular to a device for harvesting cells in a cell factory by utilizing an ultrasonic technology.

Background

The cell factory is a cell culture tool commonly used in the biological and pharmaceutical industries, and is suitable for industrial mass production of vaccines, such as hepatitis A vaccine, encephalitis B vaccine and the like. Most cells are in adherent growth in the culture process, and when the vaccine is prepared, especially for the preparation of intracellular virus vaccines with strong cell binding property, such as rotavirus, EV71 virus, CA16 virus, hepatitis A virus, herpes virus and the like, the cultured cells need to be peeled, collected and crushed from culture tools such as cell factories, spinner flasks or Kirschner flasks, and free virus particles can be obtained. At present, the following two methods are mainly used for collecting cells: the method comprises the steps of firstly, freezing a cell culture vessel in a refrigerator, taking out, thawing, and harvesting, wherein the freezing process takes long time, the virus loss is large, and if the cell culture vessel is used for collecting cells in a cell factory, a large amount of ultralow temperature preservation equipment needs to be arranged, so that the feasibility is poor; and the other is a digestive juice method, specifically, digestive juice containing digestive enzyme is added into a cell culture vessel, adhesion molecules between cells and the cell culture vessel are cut off, the cells are peeled and collected from the cell culture vessel, and introduced exogenous substances are removed through centrifugation, so that the cells are harvested. Is suitable for a cell factory system, but has the defects that digestive juice components are introduced and are difficult to remove, and has risks on the safety of vaccines. In view of the above, there is a need to develop a safe and efficient method for harvesting cells in a cell factory.

Disclosure of Invention

The invention aims to overcome the defects in the prior art, seeks to design a device for harvesting cells based on ultrasonic waves, and solves the problems of long time consumption and complex treatment process of the existing cell harvesting method.

In order to achieve the purpose, the invention adopts the following technical scheme:

an ultrasonic cell harvesting device comprises an ultrasonic device and a cell factory, wherein the vibration end of the ultrasonic device is arranged outside the cell factory, and the cell factory vibrates by generating ultrasonic waves so as to strip cells from the wall of the cell factory.

The vibration end of the ultrasonic device is tightly attached to the outer wall of the cell factory or has a certain distance with the outer wall of the cell factory.

The ultrasonic device comprises an ultrasonic generator, an ultrasonic transducer and a vibration end, wherein the ultrasonic generator, the ultrasonic transducer and the vibration end are sequentially connected, and the vibration end is in contact with the outer wall of the cell factory.

The section of the vibration end 2 is in a shape like a Chinese character 'yi' or 'C'.

An ultrasonic cell harvesting device comprises a horizontal moving device which realizes that a vibration end approaches to the outer wall of a cell factory.

The horizontal moving device comprises a platform, a horizontal guide rail and a cylinder, wherein at least one horizontal guide rail is fixed on the platform, the ultrasonic device is connected with the horizontal guide rail in a sliding manner, a piston rod of the cylinder is connected with the ultrasonic device, and under the driving of the cylinder, the ultrasonic device moves left and right along the horizontal guide rail to drive the vibration end to be close to or far away from the cell factory.

The vibrating end is capable of simultaneously generating ultrasonic waves for cell detachment to at least one layer of the cell factory.

The vibrating end is arranged at the outer side of a certain layer of the cell factory, and each layer of the cell factory is peeled off by controlling the vibrating end to move in the height direction relative to the cell factory.

The ultrasonic cell harvesting device should further comprise a vertical moving device for driving the vibrating end 2 to move along the height direction of the cell factory and stop at each layer of the cell factory.

The vertical moving device comprises a lead screw, a lead screw nut, a servo motor, a bearing, a coupler and a vertical guide rail, wherein the servo motor is connected with the lead screw in a rotating mode through the coupler and the bearing sequentially, the lead screw nut is connected with the lead screw, the vertical guide rail is arranged in parallel with the lead screw, and the platform is fixed on the lead screw nut and is connected with the vertical guide rail in a sliding mode.

The ultrasonic cell harvesting device further comprises a controller and a touch screen, wherein the controller is respectively connected with the ultrasonic generator, the air cylinder and the servo motor, the accurate control of ultrasonic power, opening time, horizontal moving distance and vertical moving distance is achieved, the touch screen is connected with the controller, and the control process is achieved by operating the touch screen.

Ultrasonic wave results cell device, still including removing the box, bottom half fixed mounting universal wheel, with ultrasonic device, the cell factory, horizontal migration device and vertical mobile device all place in the box, one side sets up the chamber that holds of placing the cell factory in the box, holding chamber bottom both sides limit fixed slide rail, take out fixed slider in the groove outside and slide rail sliding connection, it sets up the push-and-pull door to hold the chamber outside, remove the access that the cell factory was realized to the push-and-pull door, parallel placement's lead screw and vertical guide rail both ends are all fixed on the removal box that holds chamber one side.

Compared with the prior art, the invention has the following beneficial effects: the cells are stripped from the surface of the culture vessel by adopting ultrasonic oscillation, the process is simple, the time consumption is short, the efficiency is high, the pollution risk is reduced, the virus loss is less, and the virus yield is improved; in addition, partial cells can be simultaneously crushed in the ultrasonic collection process, the pressure of the subsequent crushing step is reduced, and the ultrasonic cell disruption device is particularly suitable for a cell culture tool of a cell factory in batch.

Drawings

FIG. 1 is a front view of an ultrasonic cell harvesting apparatus according to example 1.

FIG. 2 is a side view of the ultrasonic cell harvesting apparatus according to example 1.

Fig. 3 is a cross-sectional view AA of fig. 2.

Detailed Description

The present invention will be further described with reference to the following specific embodiments and accompanying drawings.

Example 1:

an ultrasonic method for harvesting cells features that the cells to be harvested are stripped from the wall of cell factory by ultrasonic waves, and the stripped cells are discharged from the liquid outlet (or liquid inlet) of cell factory along with culture liquid.

An ultrasonic cell harvesting device comprises an ultrasonic device and a cell factory 1, wherein a vibration end 2 of the ultrasonic device is arranged outside the cell factory, the vibration of the cell factory is realized by generating ultrasonic waves, and cells are further peeled from the wall of the cell factory.

The vibrating end 2 of the ultrasonic device is attached to the outer wall of the cell factory or is spaced from the outer wall of the cell factory by a predetermined distance, and in either case, the ultrasonic wave generated by the ultrasonic device can vibrate the cell factory to peel off the cells from the inner wall of the cell factory. In order to achieve a good peeling effect, the vibration end 2 of the ultrasonic device is preferably tightly attached to the outer wall of the cell factory.

The ultrasonic device comprises an ultrasonic generator 11, an ultrasonic transducer 3 and a vibration end 2, wherein the ultrasonic generator 11, the ultrasonic transducer 3 and the vibration end are sequentially connected, and the vibration end 2 is in contact with the outer wall of the cell factory 1.

Further, the shape of the vibrating end 2 is set according to actual needs, the vibrating end 2 is designed to be in a straight shape and is arranged at one side of the cell factory, and the vibrating end can also be designed to be in a C shape to semi-surround the cell factory so as to improve the stripping effect on the cells. It may be designed into other shapes as required.

When the ultrasonic cell harvesting device works, the vibration end is close to the outer wall of the cell factory 1, when the ultrasonic cell harvesting device is in contact with the outer wall of the cell factory, the ultrasonic generator 11 is started, the ultrasonic generator 11 transmits a generated high-frequency alternating current signal to the ultrasonic transducer 3, the ultrasonic transducer 3 generates high-frequency mechanical oscillation (namely ultrasonic waves) and transmits the high-frequency mechanical oscillation to the vibration end 2, so that the oscillation of culture solution and cultured cells in the cell factory is realized, and the cells are peeled off from the inner wall of the cell factory 1.

In a further embodiment, the ultrasonic cell harvesting device comprises a horizontal movement device for achieving the approach of the vibrating end 2 to the outer wall of the cell factory. The horizontal moving device comprises a platform 4, a horizontal guide rail (not shown) and a cylinder 5, wherein at least one horizontal guide rail, preferably two horizontal guide rails, is fixed on the platform 4, the ultrasonic device is connected with the horizontal guide rail in a sliding manner, specifically, the ultrasonic transducer 3 is connected with the horizontal guide rail in a sliding manner, and a piston rod of the cylinder 5 is connected with the ultrasonic transducer 3. Under the drive of cylinder 5, ultrasonic transducer 3 moves along horizontal guide left (or right), drives vibration end 2 and is close to cell factory 1, until the lateral wall contact with certain one deck of cell factory, carries out ultrasonic treatment to this layer, handles the back of accomplishing, and cylinder 5 contracts, and ultrasonic transducer 3 moves along horizontal guide right (or left), drives vibration end 2 and keeps away from cell factory 1.

In a further embodiment, the vibrating end 2 is capable of simultaneously generating ultrasonic waves for stripping cells from at least one layer of the cell factory. Since the number of layers of the cell factory is uncertain in the actual use process, it is preferable that the vibrating end is disposed outside a certain layer of the cell factory, and the peeling of each layer of the cell factory is realized by controlling the vibrating end 2 to move in the height direction with respect to the cell factory 1.

In order to achieve the above-mentioned movement in the vertical direction, the ultrasonic cell harvesting device should further include a vertical moving device for moving the vibrating end 2 along the height direction of the cell factory and stopping at each layer of the cell factory. The vertical moving device comprises a lead screw 6, a lead screw nut (not shown), a servo motor 7, a bearing 8, a coupler 9 and a vertical guide rail 10, wherein the servo motor 7 is rotatably connected with the lead screw 6 sequentially through the coupler 9 and the bearing 8, the lead screw nut is connected with the lead screw 6, the vertical guide rail 10 is arranged in parallel with the lead screw 6, and the platform 4 is fixed on the lead screw nut and is in sliding connection with the vertical guide rail 10. The servo motor 7 drives the screw nut to move up and down when working, and the platform 4 drives the vibration end 2 and the screw nut to move synchronously because the platform 4 is fixed on the screw nut, thereby realizing the movement between different layers of a cell factory.

In a further scheme, the ultrasonic cell harvesting device further comprises a controller (not shown in the figure) and a touch screen 12, wherein the controller is respectively connected with the ultrasonic generator 11, the air cylinder 5 and the servo motor 7 to realize accurate control of ultrasonic power, opening time, horizontal moving distance and vertical moving distance, the touch screen 12 is connected with the controller 11, and the control process is realized by operating the touch screen 12.

Further scheme, ultrasonic wave results cell device, still including removing the box, bottom half fixed mounting universal wheel 13, so that ultrasonic wave results cell device removes, with ultrasonic wave device, cell factory 1, horizontal migration device and vertical mobile device are all placed in the box, one side sets up the chamber that holds of placing cell factory in the box, take out groove 14 and hold chamber sliding connection, specifically, holding chamber bottom both sides limit fixed slide rail 15, take out fixed slider 16 in the groove 14 outside and slide rail 15 sliding connection, it sets up push-and-pull door 17 to hold the chamber outside, remove push-and-pull door 17 and realize the access of cell factory 1. Parallel placement's lead screw 6 and vertical guide rail 10 both ends are all fixed on the removal box that holds chamber one side, screw nut with be connected at lead screw 6, servo motor 7 loops through shaft coupling 9 and bearing 8 and is connected with lead screw 6 rotation, platform 4 is fixed on screw nut, with vertical guide rail 10 sliding connection, fixed horizontal guide rail on platform 4, 3 one end of ultrasonic transducer is connected with the piston rod of cylinder 5, ultrasonic transducer 3 slides along horizontal guide rail under the drive of cylinder 5.

In use, the extraction groove 14 is pulled out, the cell factory is placed in the extraction groove 14, and the extraction groove 14 is pushed into the accommodation cavity. Under the drive of cylinder, vibration end 2 is close to the cell factory, hug closely behind cell factory outer wall, start ultrasonic device, produce the ultrasonic wave, transmit the cell factory through vibration end 2, accomplish peeling off of cell factory internal cell, then the cylinder shrink, vibration end 2 moves along horizontal guide rail, vibration end 2 keeps away from the cell factory, reach the assigned position after, servo motor starts, vibration end 2 upwards or the distance of a cell factory of downstream along vertical guide rail, so repetition, until accomplishing peeling off of cell in the whole cell factory.

Claims

1. An ultrasonic cell harvesting device is characterized by comprising an ultrasonic device and a cell factory, wherein the vibration end of the ultrasonic device is arranged outside the cell factory, and the vibration of the cell factory is realized by generating ultrasonic waves so as to strip cells from the wall of the cell factory; the vibration end of the ultrasonic device is tightly attached to the outer wall of the cell factory or has a certain distance with the outer wall of the cell factory.

2. The ultrasonic cell harvesting device of claim 1, wherein the ultrasonic device comprises an ultrasonic generator, an ultrasonic transducer and a vibrating end, the ultrasonic generator, the ultrasonic transducer and the vibrating end are connected in sequence, and the vibrating end is in contact with the outer wall of the cell factory.

3. The ultrasonic cell harvesting device of claim 2, wherein the ultrasonic cell harvesting device comprises a horizontal moving device for moving the vibrating end toward the outer wall of the cell factory.

4. The ultrasonic cell harvesting device of claim 3, wherein the horizontal moving device comprises a platform, a horizontal guide rail and a cylinder, at least one horizontal guide rail is fixed on the platform, the ultrasonic device is connected with the horizontal guide rail in a sliding manner, a piston rod of the cylinder is connected with the ultrasonic device, and the ultrasonic device moves left and right along the horizontal guide rail under the driving of the cylinder to drive the vibration end to approach or leave the cell factory.

5. The ultrasonic cell harvesting device of claim 4, wherein the vibrating end is capable of simultaneously generating ultrasonic waves to peel off cells from at least one layer of the cell factory.

6. The ultrasonic cell harvesting device of claim 4, wherein the vibrating end is disposed outside a layer of the cell factory, and the peeling of each layer of the cell factory is performed by controlling the vibrating end to move in the height direction of the cell factory, and the ultrasonic cell harvesting device further comprises a vertical moving device for moving the vibrating end in the height direction of the cell factory and stopping at each layer of the cell factory.

7. The ultrasonic cell harvesting device of claim 6, wherein the vertical moving device comprises a screw, a screw nut, a servo motor, a bearing, a coupler and a vertical guide rail, the servo motor is rotatably connected with the screw through the coupler and the bearing in sequence, the screw nut is connected with the screw, the vertical guide rail is parallel to the screw, and the platform is fixed on the screw nut and is slidably connected with the vertical guide rail.

8. The ultrasonic cell harvesting device of claim 7, further comprising a controller and a touch screen, wherein the controller is connected to the ultrasonic generator, the cylinder and the servo motor respectively to control the ultrasonic power, the turn-on time, the horizontal movement distance and the vertical movement distance precisely, and the touch screen is connected to the controller and operated by the touch screen to realize the above control process.

9. The ultrasonic cell harvesting device of claim 8, further comprising a movable box, wherein universal wheels are fixedly mounted at the bottom of the box, the ultrasonic device, the cell factory, the horizontal moving device and the vertical moving device are all placed in the box, a containing cavity for placing the cell factory is formed in one side in the box, slide rails are fixed on two side edges of the bottom of the containing cavity, a slide block fixed on the outer side of the drawing groove is slidably connected with the slide rails, a sliding door is arranged on the outer side of the containing cavity, the cell factory is stored and taken out by moving the sliding door, and two ends of a lead screw and two ends of a vertical guide rail which are placed in parallel are fixed on the movable box on one side of the containing cavity.