CN211842229U

CN211842229U - Novel marked gel electrophoresis gel recovery and cutting device

Info

Publication number: CN211842229U
Application number: CN202020209144.9U
Authority: CN
Inventors: 潘聪; 冯韵宇
Original assignee: Chengdu Yibang Pharmaceutical Technology Co ltd
Current assignee: Chengdu Yibang Pharmaceutical Technology Co ltd
Priority date: 2020-02-25
Filing date: 2020-02-25
Publication date: 2020-11-03
Anticipated expiration: 2030-02-25

Abstract

The utility model relates to a molecular biology equipment field relates to gel electrophoresis glues and retrieves cutting device, but concretely relates to novel gel electrophoresis of mark glues retrieves cutting device, including both ends open-ended recovery tube and the boost motor that is used for injecting air into to the recovery tube, the opening of the one end of recovery tube is for being used for cutting the cutting opening that gel electrophoresis glued, and the opening of the other end of recovery tube is for the connector with the boost motor intercommunication, and the recovery tube can be dismantled with the boost motor. The device can cut and recover the gel electrophoresis gel containing the target gene DNA fragment rapidly and safely. The device can be applied to molecular biology experimental research, and can ensure the safety and the accuracy of gel recovery operation.

Description

Novel marked gel electrophoresis gel recovery and cutting device

Technical Field

The utility model relates to a molecular biology equipment field relates to gel electrophoresis and glues recovery cutting device, and concretely relates to novel gel electrophoresis that can mark glues recovery cutting device.

Background

With the increase of the investment of China in the field of biological medicine, more and more biological medicine related laboratories appear. In the research of biochemical analysis and test, gel electrophoresis separation has become the most common means for detection experiments such as PCR and enzyme digestion identification. The gel electrophoresis method is the simplest, rapid, economical and practical method for separating the DNA fragment of the target gene which is wanted by people. In the prior art, the method for cutting and recovering the target gene DNA fragment from the gel electrophoresis gel comprises the following steps: putting the gel electrophoresis gel into an ultraviolet dark box, developing the DNA band under the irradiation of an ultraviolet lamp because the gel electrophoresis gel contains ethidium bromide EB nucleic acid dye with carcinogenic toxicity, finally manually cutting off the part containing the target gene DNA fragment by using a blade, putting the cut gel into a microcentrifuge tube (for example, a 0.5ml EP tube), and then carrying out subsequent separation and purification operation on the target gene DNA fragment. Cutting the gel with a blade often requires four or more knives, which is time consuming, cumbersome and imprecise, also tends to shred the gel, and the blade edge is sharp and easily injurious to humans. The overlong ultraviolet irradiation time not only damages the basic group and influences the accuracy of the experiment, but also easily causes harm to people after long-time contact. In addition, during electrophoresis gel running, a plurality of samples in a pore passage often occur, and when the gel containing the target gene DNA fragment is cut and recovered, the sample names are easy to be confused. Therefore, it is necessary to design a gel recycling and cutting device which has a simple structure, is safe and feasible, is easy to operate, and is accurate and efficient.

SUMMERY OF THE UTILITY MODEL

The utility model aims to solve the technical problem that a novel gel electrophoresis that can mark is glued and is retrieved cutting device is provided, and the device can avoid using the blade, cuts and retrieves the gel electrophoresis that contains target gene DNA fragment fast and safely and glues.

For solving above-mentioned technical problem, this technical scheme provides but novel gel electrophoresis of mark glues recovery cutting device, including both ends open-ended recovery tube and the boost motor that is used for injecting the air into the recovery tube, the opening of the one end of recovery tube is for being used for cutting the cutting opening that gel electrophoresis glued, and the opening of the other end of recovery tube is for the connector with the boost motor intercommunication, and the recovery tube can be dismantled with the boost motor.

By adopting the technical scheme, the technical principle and the beneficial effects are as follows: the recovery tube is vertically placed above the electrophoresis gel, one end of the recovery tube is provided with a cutting opening, the cutting opening is aligned to the position of the target DNA strip, the cutting opening can surround the DNA strip to be recovered to cut the electrophoresis gel (generally agarose gel), and the gel with the target gene DNA fragment can be cut off by only one-time cutting. And the electrophoresis gel has certain viscosity, can form certain adhesion with the inner wall of the recovery tube, and can take out the gel adhered inside the recovery tube and provided with the target gene DNA fragment only by taking out the recovery tube. In order to conveniently take out the gel from the recovery tube and put the gel into a microcentrifuge tube (such as an EP tube), a booster (a common syringe can be used) is fixedly connected with the connecting port, the cutting port is arranged above the microcentrifuge tube, the booster is used for inflating the recovery tube, so that the gel with the target gene DNA fragment falls into the microcentrifuge tube, and then the next separation, purification and recovery operation is carried out. Since the cutting operation is performed in an ultraviolet chamber (e.g., a dark chamber type ultraviolet analyzer), the space inside the chamber is limited and the light is dim (only a small amount of ultraviolet light and fluorescence from the DNA fragments), if the recovery tube and the booster are not detachable, the booster occupies a large space, which is inconvenient for the user to hold and perform the cutting operation. In addition, the booster can also block light, so that a user cannot conveniently observe the position of the DNA strip, and the cutting accuracy is influenced. In addition, the booster and the recovery tube are detachably arranged, so that the recovery tube (which is a disposable article) can be replaced before each operation, the samples (gel with different DNA fragments) are prevented from being mixed, and the cross contamination among the samples is prevented.

Further, the cutting opening is oval.

By adopting the technical scheme, the gel volume cut by the rectangular cutting opening is smaller (namely the length of the long axis of the ellipse is consistent with the length of the long edge of the rectangle) compared with the gel volume cut by the rectangular cutting opening with the same length, the recovered gel volume is smaller, the subsequent purification operation is easier, and the purification rate is higher.

Further, the recovery pipe is made of transparent plastic.

By adopting the technical scheme, the recovery pipe is made of plastic, the cost is lower, and the plastic is suitable for being used as a material of disposable articles. The recovery tube is a disposable article, so that the samples (gels with different DNA fragments) are prevented from being mixed, and cross contamination among the samples is prevented. In addition, the transparent recovery tube can prevent the recovery tube from shielding fluorescence emitted by the DNA strip in the glue cutting operation, and is more convenient for observation and accurate cutting.

Further, the outer surface of the recovery pipe is provided with a marking area.

By adopting the technical scheme, the confusion of sample marking can be avoided.

Further, a label plate for writing and blocking light is fixed on the marking area.

By adopting the technical scheme, the label plate is convenient to write. The label plate has a light blocking function, and the position of the recovery tube can be observed conveniently in the ultraviolet box. If the entire recovery tube is transparent, it may be difficult to distinguish the location of the recovery tube in a dark uv chamber (only the DNA strip in the gel at the bottom of the uv chamber fluoresces under uv excitation, and in addition, uv light is not visible and has little illumination effect). If a small light blocking structure is arranged in the transparent recovery pipe to form contrast, the position of the recovery pipe can be judged easily.

Further, the major axis of the cuts was 5mm and the minor axis was 3 mm.

By adopting the technical scheme, the cutting opening is smaller than the caliber of the micro centrifugal tube, so that the cut gel can be conveniently put into the micro centrifugal tube. The above arrangement allows the cutting port to cut the gel containing DNA sufficiently while avoiding cutting to obtain an excessive gel containing no DNA fragments.

Further, the booster comprises a cylinder and a piston structure; one end of the cylinder is communicated with the recovery pipe, and the piston structure is inserted into the other end of the cylinder and is used for pushing air into the recovery pipe from the inside of the cylinder.

By adopting the technical scheme, the gel can fall into the micro centrifugal tube by injecting air into the recovery tube from the booster.

Further, the piston structure comprises a piston head, a piston push rod and a piston push handle; the piston head is connected with the inner surface of the cylinder body in a sealing and sliding mode, one end of the piston push rod is fixedly connected with the piston head, and the other end of the piston push rod is fixedly connected with the piston push handle.

By adopting the technical scheme, the piston push rod and the piston push handle are arranged to facilitate pushing the piston head to convey gas.

Further, the barrel comprises a barrel body, a conical base and a connector which are integrally formed and sequentially communicated, and the connector is used for being inserted into the connector and enabling the barrel to be communicated with the recovery pipe.

By adopting the technical scheme, the connector and the connector are connected in an inserting mode, the structure is simple, and the fixing and the disassembling operation are convenient.

Further, the piston head comprises a piston body, a conical plug and a conical head which are fixedly connected in sequence, the piston body is hermetically and slidably connected with the inner surface of the cylinder body, the shape of the conical plug is matched with the inner surface of the side wall of the conical base, and the conical head is fixed at the conical tip of the conical plug and is used for being inserted into the connector.

Adopt above-mentioned technical scheme, the setting of toper stopper and conical head can fully extrude the air in the section of thick bamboo body, ensures to squeeze into the gel in the miniature centrifuging tube.

Drawings

Fig. 1 is a schematic perspective view of a recovery pipe according to embodiment 1 of the present invention.

Fig. 2 is a bottom view of the recovery pipe according to embodiment 1 of the present invention.

Fig. 3 is a front view of the novel markable gel electrophoresis gel recovery cutting device of embodiment 1 of the present invention.

Fig. 4 is a front view of a booster according to embodiment 1 of the present invention.

Fig. 5 is a schematic perspective view of a recovery pipe according to embodiment 2 of the present invention.

Detailed Description

The following is further detailed by way of specific embodiments:

reference numerals in the drawings of the specification include: the recycling pipe comprises a recycling pipe 1, a connecting port 2, a cutting port 3, a cylinder body 4, a connecting head 5, a conical base 6, a conical head 7, a conical plug 8, a piston body 9, a piston push rod 10, a piston push handle 11 and a label plate 12.

Example 1

Novel gel electrophoresis gel recovery cutting device can mark, and the treatment structure includes adjusts the structure, as shown in fig. 3, including both ends open-ended recovery tube 1 and the booster that is used for injecting the air to recovery tube 1. As shown in fig. 1 and 2, the recovery pipe 1 is a conical short pipe with gradually changing pipe diameter. As shown in fig. 1, the lower end opening of the recovery pipe 1 is a cutting opening 3 for gel cutting, and the upper end opening of the recovery pipe 1 is a connection opening 2 for connection with the booster. As shown in FIG. 2, the cutting opening 3 and the connection opening 2 are both elliptical, wherein the major axis of the cutting opening 3 is 5mm, the minor axis is 3mm, the size of the cutting opening 3 is smaller than the caliber of the micro-centrifuge tube and larger than the size of the DNA fragment on the electrophoresis gel, so as to ensure that all the DNA fragments can be cut and obtained and the gel can be conveniently put into the micro-centrifuge tube. The recycling pipe 1 is made of transparent plastic, specifically polypropylene plastic, and is common plastic for centrifuge tubes and the like in molecular biology research. The recycling pipe 1 is made of plastic, so that the cost is low, and the plastic is suitable for being used as a material of disposable articles. The recovery tube 1 is a disposable item, which prevents the mixing of samples (gels with different DNA fragments) and prevents cross contamination between samples. In addition, the transparent recovery tube 1 can prevent the recovery tube 1 from shielding fluorescence emitted by the DNA strip in the gel cutting operation, thereby being more convenient for observation and accurate cutting. In order to make the device easy to handle during the glue cutting operation, the distance between the connection port 2 and the cutting port 3 may be set to 3-5cm (i.e., the length range of the recovery pipe).

As shown in fig. 3, the booster is of a needle cylinder structure and comprises a cylinder body and a piston structure, the cylinder body comprises a cylinder body 4, a conical base 6 and a connector 5 which are integrally formed and sequentially communicated, the cylinder body 4 is a right cylindrical cylinder and has openings at two ends, the conical base 6 is a conical pipe and has openings at two ends, and the connector 5 is also a cylinder with openings at two ends (the cross section is oval). The lower end of the cylinder body 4 is connected with an opening at the bottom of the cone base 6, and the upper end of the connector 5 is connected with an opening at the top of the cone base 6. The shape of the connector 5 is matched with that of the connecting port 2, the connector 5 can be inserted and fixed in the connecting port 2, and the air tightness of the contact part of the connector 5 and the connecting port 2 is ensured (the connector 5 is clamped and fixed in the connecting port 2 to form a sealed connection). The piston structure comprises a piston head, a piston push rod 10 and a piston push handle 11, wherein one end of the piston push rod 10 is bonded with the piston head, and the other end of the piston push rod is bonded with the piston push handle 11. The piston head comprises a piston body 9, a conical plug 8 and a conical head 7 which are sequentially bonded, the piston body 9 is cylindrical, a rubber layer is wrapped on the outer layer of the piston body to guarantee air tightness, the periphery of the piston body 9 is in sliding connection with the inner surface of the cylinder body 4, and the piston body 9 slides along the axial direction of the cylinder body 4. The lower end of the piston body 9 is bonded with the conical bottom of the conical plug 8, the shape of the conical plug 8 is matched with the inner surface of the side wall of the conical base 6, and the conical head 7 is a solid cylinder and is fixed at the conical tip of the conical plug 8. When the piston structure is pushed to the lowest end of the cylinder, the conical head 7 is inserted into the connecting head 5, the conical surface of the conical plug 8 contacts with the inner surface of the side wall of the conical base 6 (as shown in fig. 4), and the air in the cylinder is completely exhausted.

The specific implementation process is as follows: when the gel electrophoresis gel is required to be cut, the gel electrophoresis gel is placed in an ultraviolet box, a fluorescence signal of a DNA strip is observed from an observation port at the upper part of the ultraviolet box, then a hand is inserted into the ultraviolet box, the recovery tube 1 is held by hand, the recovery tube 1 is vertically placed above the electrophoresis gel, the cutting port 3 is aligned to the position of the target DNA strip, the cutting port 3 can wrap the DNA strip to be recovered to cut the electrophoresis gel (generally agarose gel), and the gel with the target gene DNA fragment can be cut by only one-time cutting. The recovery tube 1 is then taken out of the ultraviolet box, at which time the electrophoretic glue adheres to the inner wall of the recovery tube 1. Then transfer the piston structure of boost motor to the one end of keeping away from connector 5, insert connector 5 again in connector 2 to with cutting opening 3 alignment micro-centrifuge tube's upper portion opening, promote piston structure, transfer the gel to the micro-centrifuge tube in through atmospheric pressure. The recovery tube 1 is a disposable tube and can be discarded after being cut once (since the gel contains toxic substances such as EB and the like, the gel needs to be discarded to a special experimental waste disposal place). If the recovery pipe 1 is reused, the recovery pipe 1 needs to be cleaned in order to avoid cross contamination, and the water source is seriously polluted by the waste water generated by cleaning because the gel contains toxic substances such as EB and the like. The operation method of the scheme can avoid the water source pollution. In addition, the recovery tube 1 is a disposable tube and can be discarded after being cut once, so that cross contamination among different samples is avoided, confusion of different DNA fragments is avoided, and the accuracy of the experiment is increased.

Example 2

This example is basically the same as example 1, except that, as shown in FIG. 5, a label plate 12 is further stuck to the outer side of the side wall of the collection tube 1, the label plate 12 is a white plastic plate (white PVC plastic plate), and the name of the sample (for example, the length of DNA, the name of a representative gene, etc.) can be marked on the label plate 12 to avoid confusion of the sample. The white plastic plate is small and opaque, facilitating the viewing of the position of the recovery tube 1 in the uv-box. If the entire recovery pipe 1 is transparent, in a dark ultraviolet box, it may be difficult to distinguish the position of the recovery pipe 1. If a small light blocking structure is arranged in the transparent recycling pipe 1 for comparison, the position of the recycling pipe 1 can be judged easily.

While the embodiments of the present invention have been described in detail with reference to the accompanying drawings, it is to be understood that the present invention is not limited to the embodiments and that various changes and modifications may be made therein by those skilled in the art without departing from the spirit and scope of the invention. These should also be considered as the scope of protection of the present invention, and these do not affect the effect of the implementation of the present invention and the utility of the patent. The techniques, shapes, and structural parts, which are omitted from the description of the present invention, are all known techniques.

Claims

1. Novel gel electrophoresis of can marking is glued and is retrieved cutting device, its characterized in that: including both ends open-ended recovery tube and the booster that is used for injecting the air into the recovery tube, the opening of the one end of recovery tube is for being used for cutting the cutting mouth that gel electrophoresis glued, and the opening of the other end of recovery tube is the connector that communicates with the booster, and the recovery tube can be dismantled with the booster.

2. The markable gel electrophoresis gel recovery cutting device according to claim 1, characterized in that: the cutting opening is oval.

3. The markable gel electrophoresis gel recovery cutting device according to claim 2, characterized in that: the recovery pipe is made of transparent plastic.

4. The markable gel electrophoresis gel recovery cutting device according to claim 3, characterized in that: the outer surface of the recovery pipe is provided with a marking area.

5. The markable novel gel electrophoresis gel recovery cutting device according to claim 4, characterized in that: and a label plate for writing and blocking light is fixed on the marking area.

6. The markable novel gel electrophoresis gel recovery cutting device according to claim 5, characterized in that: the major axis of the cut is 5mm and the minor axis is 3 mm.

7. The markable novel gel electrophoresis gel recovery cutting device according to claim 6, characterized in that: the booster comprises a cylinder body and a piston structure; one end of the cylinder is communicated with the recovery pipe, and the piston structure is inserted into the other end of the cylinder and is used for pushing air into the recovery pipe from the inside of the cylinder.

8. The markable gel electrophoresis gel recovery cutting device according to claim 7, characterized in that: the piston structure comprises a piston head, a piston push rod and a piston push handle; the piston head is connected with the inner surface of the cylinder body in a sealing and sliding mode, one end of the piston push rod is fixedly connected with the piston head, and the other end of the piston push rod is fixedly connected with the piston push handle.

9. The markable gel electrophoresis gel recovery cutting device of claim 8, wherein: the barrel comprises a barrel body, a conical base and a connector which are integrally formed and sequentially communicated, and the connector is used for being inserted into the connector and communicating the barrel with the recovery pipe.

10. The markable novel gel electrophoresis gel recovery cutting device according to claim 9, characterized in that: the piston head comprises a piston body, a conical plug and a conical head which are fixedly connected in sequence, the piston body is hermetically and slidably connected with the inner surface of the cylinder body, the shape of the conical plug is matched with the inner surface of the side wall of the conical base, and the conical head is fixed at the conical tip of the conical plug and is used for being inserted into the connector.