CN211793997U - Medical biomaterial low temperature save set - Google Patents
Medical biomaterial low temperature save set Download PDFInfo
- Publication number
- CN211793997U CN211793997U CN201922502059.1U CN201922502059U CN211793997U CN 211793997 U CN211793997 U CN 211793997U CN 201922502059 U CN201922502059 U CN 201922502059U CN 211793997 U CN211793997 U CN 211793997U
- Authority
- CN
- China
- Prior art keywords
- solution
- temperature
- liquid nitrogen
- control part
- concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medical Preparation Storing Or Oral Administration Devices (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a medical biomaterial low temperature save set for the high and conventional technique of activity requirement is difficult to the biomaterial that satisfies the requirement after various rewarming of low temperature preservation. Present biomaterial carries out the low temperature protection loading and elutes and adopts manual operation, wastes time and energy, the step is loaded down with trivial details, makes mistakes easily, the utility model provides a medical biomaterial low temperature storage device, the utility model discloses mainly constitute by two parts: the temperature control part of the solution enables the temperature of the cryoprotectant to be reduced or increased according to a set time curve in a gradient manner; the solution concentration control part increases or decreases the concentration of the cryoprotectant according to a set time curve gradient; the solution temperature control part and the solution concentration control part share an industrial control computer and a data acquisition control interface board for matching and regulation. The utility model discloses can make biomaterial carry out automatic cryoprotectant loading/elution under controllable temperature and concentration condition, avoid the manual operation defect.
Description
Technical Field
The utility model relates to a medical biomaterial low temperature save set for the high and conventional technique of activity requirement is difficult to the biomaterial that satisfies the requirement after various rewarming of low temperature preservation.
Background
Cryopreservation of organisms is generally divided into freezing and vitrification methods, wherein vitrification methods both employ rapid temperature raising and lowering techniques, which are feasible for suspended cells or thin-layered tissues, but difficult to achieve for tissues or organs with greater thickness. For biological tissues of a certain size, the time it takes for the cryoprotectant to enter cells in its central location is much longer than it takes to enter thin layers of tissue or suspended cells, and the problem of toxic damage is more severe. In order to reduce toxic damage, the characteristic that the toxicity of the cryoprotectant is reduced along with the reduction of concentration and temperature can be utilized, when the cryoprotectant is loaded into the biological material, a mode of increasing the concentration while reducing the temperature is adopted, the concentration required by vitrification is finally achieved, when the cryoprotectant is eluted from the interior of the biological material, a mode of increasing the temperature while reducing the concentration is adopted, and the concentration is finally made to be close to zero, so that a low-temperature preservation mode of a liquid phase line tracking method is developed. However, the loading/elution of the cryoprotectant during the low-temperature protection of the existing biological material adopts manual operation, which is complicated in steps, labor-consuming, and prone to error in manual operation, thereby causing failure of low-temperature preservation of the organism and bringing great loss to scientific research.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model and the technical task that provides overcome current biomaterial and carry out low temperature protection loading and elution and adopt manual operation, waste time and energy, the step is loaded down with trivial details, the error of makeing mistakes easily leads to the shortcoming of biological preservation failure, a medical biomaterial low temperature save set is provided, the device can make biomaterial carry out automatic cryoprotectant loading/elution under controllable temperature and concentration condition, realize preserving bioactivity better, avoid all sorts of defects that manual operation brought.
The utility model discloses the technical scheme who adopts: a medical biomaterial low-temperature storage device mainly comprises a solution concentration control part and a solution temperature control part, wherein the solution concentration control part comprises a plurality of solution storage tanks, a multi-way switching valve, a peristaltic pump, a cryopreservation container, a waste liquid collecting barrel, a data acquisition control interface board, an industrial control computer and a solution conveying pipe; the solution temperature control part comprises the freezing container, a temperature sensor, a liquid nitrogen tank, a heat exchanger, an electromagnetic valve, a stirring fan, an electric heater, the data acquisition control interface board, the industrial control computer, an insulation can, a liquid nitrogen delivery pipe and a nozzle; the solution storage tank, the multi-way switching valve, the peristaltic pump, the solution side of the heat exchanger, the cryopreservation container and the waste liquid collecting barrel are sequentially connected by a solution conveying pipe; the liquid nitrogen side of the liquid nitrogen tank, the electromagnetic valve and the heat exchanger are connected in sequence by a liquid nitrogen conveying pipe; the industrial control computer is connected with a data acquisition control interface board, and the data acquisition control interface board is connected with the multi-way switching valve, the electromagnetic valve, the electric heater and the temperature sensor; the nozzle is positioned at the bottom of the heat preservation box, and the stirring fan is positioned at the top of the heat preservation box. The utility model discloses mainly constitute by two parts: the temperature control of the solution and the concentration of the solution, wherein the temperature control part of the solution is used for enabling the temperature of the cryoprotectant to be gradually reduced or increased according to a set time curve; the solution concentration control part is used for enabling the concentration of the cryoprotectant to increase or decrease according to a set time curve gradient; the solution temperature control part and the solution concentration control part share an industrial control computer and a data acquisition control interface board, the setting of the change curve of the temperature and the concentration of the cryoprotectant along with time is implemented by software of the computer, and the software control is conventional technology for the technicians in the field and is not described again. The utility model discloses adjust the temperature of freezing the interior solution of depositing the container through the measure of solution concentration control and solution temperature control these two parts during the use, the heat exchanger through the liquid nitrogen cooling makes cryoprotectant solution drop to the settlement temperature before the container is deposited to the injection freezing on the one hand, and on the other hand spouts the space temperature that the insulation can made around the sample container into with the liquid nitrogen and maintains near settlement temperature. When the low-temperature protective agent is loaded, the method for increasing the concentration of the low-temperature protective agent is to pump the low-temperature protective agent solution with higher concentration into the cryopreservation container and pump out the original solution in the cryopreservation container; the concentration reduction method is that the low-temperature protective agent solution with lower concentration is pumped into the freezing container, meanwhile, the original solution in the freezing container is pumped out, and the new solution can be kept for a preset period of time after entering the freezing container.
As a further improvement and supplement to the technical proposal, the utility model adopts the following technical measures that the quantity of the solution storage tanks is determined according to the low-temperature preservation proposal of the biological materials, the quantity is 2-10, and the maximum path number of the multi-path switching valve is 10.
The insulation can is internally provided with a bracket, and the freezing container is placed on the bracket and can be taken out from the bracket. The support is arranged in the heat preservation box to place the freezing container, the freezing container can be conveniently taken out from the support, the biological material is placed in the freezing container to be cooled, after the concentration of the low-temperature protective agent solution in the biological material reaches the concentration required by vitrification, the freezing container is transferred to a low-temperature refrigerator or a liquid nitrogen tank for storage, but the amount of liquid nitrogen sprayed into the heat preservation box can be increased when the condition is not met, so that the temperature in the box reaches the temperature level of the low-temperature refrigerator.
The freezing solution delivery pipe between deposit container and the waste liquid collecting vessel on connect one section nonrust steel pipe, the solution inlet side that has electric heating wire and is located the peristaltic pump is wound outward to the nonrust steel pipe, electric heating wire and a temperature controller link to each other. Because the temperature of the solution discharged from the heat insulation box can be as low as minus 50 ℃, in order to ensure the normal operation of the peristaltic pump, the temperature of the solution discharged from the heat insulation box can be increased through the stainless steel pipe with the electric heating wire. The electric heating wire can be heated by adopting 24V direct current and is adjusted and controlled by a temperature controller.
The temperature sensors are four, wherein the first temperature sensor is arranged at a solution side outlet of the heat exchanger, the second temperature sensor is arranged on a solution outlet pipeline of the cryopreservation container, the third temperature sensor is arranged in the incubator, and the fourth temperature sensor is arranged on a liquid nitrogen conveying pipeline connected with the electromagnetic valve. The temperature of each part is measured in real time through four temperature sensors, so that the temperature of the cryoprotectant loaded each time can be accurately controlled.
The utility model discloses an automatic change cooperative control solution temperature control and solution concentration, specifically the in-process concentration of cryoprotectant at the cooling is increasing gradually, and the in-process concentration that heaies up is the in-process concentration that reduces gradually, and at the cooling or arbitrary moment of hearing up, the temperature of solution all is higher than the freezing temperature of solution.
Drawings
FIG. 1: fig. 1 is a schematic diagram of the present invention.
In the figure: the system comprises a solution storage tank 1-1, a solution storage tank 1-2, a solution storage tank 1-5, a solution storage tank 1-6, a multi-way switching valve 2, a liquid nitrogen tank 3, a peristaltic pump 4, an electromagnetic valve 5-1, an electromagnetic valve 5-2, a waste liquid collecting barrel 6, a heat exchanger 7, an industrial control computer 8, a data acquisition control interface board 9, a stirring fan 10, an electric heater 11, a freezing storage container 12, a bracket 13, an incubator 14, a nozzle 15, a first temperature sensor 16, a second temperature sensor 17, a third temperature sensor 18, a fourth temperature sensor 19 and a stainless steel pipe 20.
Detailed Description
The invention will be further described with reference to the following description and embodiments in conjunction with the accompanying drawings.
As shown in the attached drawings, the medical biomaterial low-temperature storage device comprises a plurality of solution storage tanks (1-1, 1-2, 1-5 and 1-6, only 4 of which can be up to 10), a multi-way switching valve 2 (the maximum way number is 10), a liquid nitrogen tank 3 (a self-pressurization type or electric pressurization type liquid nitrogen tank which is provided with a pressure gauge and a safety valve installed by a manufacturer), a peristaltic pump 4 (a double-channel type, when peristaltic pump pipes with the same specification are used, the solution flow rates of the two channels are equal), an electromagnetic valve 5-1 (a low-temperature type, namely, the electromagnetic valve can operate for a long time without failure at the liquid nitrogen temperature), an electromagnetic valve 5-2, a waste liquid collecting barrel 6, a heat exchanger 7, an industrial control computer 8, a data acquisition control interface board 9, an insulation can 14, an electric heater 11, a freezing container 12, a first temperature sensor 16 (a sensor capable of measuring the liquid nitrogen temperature, such as a platinum resistor, a T-type thermocouple and a K-type thermocouple, the comprehensive temperature measurement accuracy of which reaches +/-0.5 ℃ after the conversion of the data acquisition control interface board, a second temperature sensor 17, a third temperature sensor 18 and a fourth temperature sensor 19; the top end of the liquid nitrogen tank 3 is provided with a safety valve, a support for placing the cryopreservation container 12 is arranged in the heat insulation box 14, the solution storage tank is connected with the multi-way switching valve 2 in parallel, the outlet of the multi-way switching valve 2 is connected to one channel of the two-channel peristaltic pump 4, the outlet of the channel is connected with the solution side inlet of the heat exchanger 7, the solution side outlet of the heat exchanger 7 is connected with the cryopreservation container 12, the solution outlet pipe of the cryopreservation container 12 is connected with the other channel of the two-channel peristaltic pump 4, and the outlet of the channel; the liquid nitrogen tank 3 is connected with electromagnetic valves in parallel, wherein the outlet of one electromagnetic valve 5-1 is connected with a nozzle 15 to spray liquid nitrogen into the heat-preserving box 14, the outlet of the other electromagnetic valve 5-2 is connected to the liquid nitrogen side of the heat exchanger 7, and the liquid nitrogen is changed into nitrogen gas after being cooled to be discharged to the atmosphere; the first temperature sensor 16 is arranged at the solution side outlet of the heat exchanger 7, the second temperature sensor 17 is arranged on the solution outlet pipeline of the freezing container 12, the third temperature sensor 17 is arranged in the insulation can 14, the fourth temperature sensor T4 is arranged on the liquid nitrogen conveying pipeline connected with the electromagnetic valve, the signals of the temperature sensors enter the data acquisition control interface board 9, the data acquisition control interface board 9 is connected with the industrial control computer 8, and the data acquisition control interface board 9 is connected with the multi-way switching valve 2, the electromagnetic valve and the electric heater 11. In addition, a section of stainless steel tube 20 is connected to the solution conveying pipe between the freezing container 12 and the waste liquid collecting barrel 6, an electric heating wire is wound outside the stainless steel tube and is positioned at the solution inlet side of the peristaltic pump 4, and the electric heating wire is connected with a temperature controller (not shown).
The utility model discloses mainly constitute by two parts: the solution temperature control and the solution concentration cooperative control mean that the changes of concentration and temperature have a restriction relationship, specifically, the concentration is gradually increased in the process of temperature reduction, the concentration is gradually decreased in the process of temperature increase, and the temperature of the solution is higher than the freezing temperature of the solution at any time of temperature reduction or temperature increase.
The solution temperature control part is used for enabling the temperature to be reduced or increased according to a set time curve gradient and comprises the freezing container 12, a temperature sensor, a liquid nitrogen tank 3, a heat exchanger 7, an electromagnetic valve 5, a stirring fan 10, an electric heater 11, a data acquisition control interface board 9, the industrial control computer 8, an incubator 14, a liquid nitrogen delivery pipe and a nozzle 15.
The solution concentration control part is used for increasing or reducing the concentration according to a set time curve gradient and comprises a plurality of solution storage tanks, a multi-way switching valve 2, a peristaltic pump 4, a freezing storage container 12, a waste liquid collecting barrel 6, a data acquisition control interface board 9, an industrial control computer 8 and a solution conveying pipe.
The solution temperature control part and the solution concentration control part share an industrial control computer and a data acquisition control interface board, and the setting of the change curves of the temperature and the concentration along with the time is implemented by software of the computer. The software is conventional to those skilled in the art and will not be described in detail.
Use before the device carries out biomaterial's cryo-preservation, at first need rationally select the kind of cryoprotectant according to biomaterial's nature, secondly need confirm cryoprotectant loading, elution step according to cryoprotectant biomaterial's osmotic characteristic, each step includes quantitative parameters such as protectant concentration, temperature and duration, and the corresponding relation between cryoprotectant solution freezing point temperature and the protectant concentration is the basis of carrying out this work.
The working process for the cryoprotectant loading of the biological material is as follows. The biological material is initially placed in a preservation solution without a low-temperature protective agent and placed in a freezing container, the freezing container is placed on a bracket in a heat preservation box, and a solution conveying pipe is connected to the freezing container; under the control of a computer, a peristaltic pump pumps out a solution in a low-concentration low-temperature protective agent solution storage tank with the concentration of x1, the solution is cooled to a certain temperature Ts1 which is higher than the freezing point of the solution through a heat exchanger and is sent to a freezing container, the solution conveying capacity is slightly more than the solution quantity required by the original solution in the freezing container which is completely replaced, the operation of the peristaltic pump is stopped after the conveying capacity meets the requirement, and simultaneously,
spraying the biological material into liquid nitrogen in an incubator through an electromagnetic valve and a nozzle under the control of a computer to enable the temperature in the incubator to reach Ts1, and treating the biological material in a low-temperature protective agent solution with the temperature of Ts1 and the concentration of x1 for a set time t 1; under the control of a computer, the peristaltic pump is started again, the low-temperature protective agent solution with the concentration of x2 (x 2> x 1) is pumped out of the storage tank, cooled to a certain temperature Ts2 higher than the freezing point of the solution through a heat exchanger and sent to a freezing container, the solution conveying amount is slightly more than the solution amount required by the original solution in the freezing container to be replaced completely, the operation of the peristaltic pump is suspended after the conveying amount meets the requirement, meanwhile, under the control of the computer, liquid nitrogen is sprayed into the heat preservation tank through an electromagnetic valve and a nozzle, the temperature in the tank reaches Ts2, and the biological material is treated in the low-temperature protective agent solution with the temperature of Ts2 and the concentration of x2 for set time t 2; under the control of a computer, the steps are repeated, the concentration of the cryoprotectant solution is continuously increased, the temperature of the cryoprotectant solution is reduced until the final concentration xn and the temperature Tsn are reached, and the biological material is processed in the solution for a time tn; and taking out the frozen container from the incubator, and storing the frozen container in a low-temperature refrigerator or a liquid nitrogen container.
Claims (4)
1. A medical biomaterial low-temperature storage device is characterized by comprising a solution concentration control part and a solution temperature control part, wherein the solution concentration control part comprises a plurality of solution storage tanks, a multi-way switching valve (2), a peristaltic pump (4), a cryopreservation container (12), a waste liquid collecting barrel (6), a data acquisition control interface board (9), an industrial control computer (8) and a solution conveying pipe; the solution temperature control part comprises the freezing container (12), a temperature sensor, a liquid nitrogen tank (3), a heat exchanger (7), an electromagnetic valve (5), a stirring fan (10), an electric heater (11), the data acquisition control interface board (9), the industrial control computer (8), an incubator (14), a liquid nitrogen delivery pipe and a nozzle (15); the solution storage tank, the multi-way switching valve (2), the peristaltic pump (4), the solution side of the heat exchanger (7), the cryopreservation container (12) and the waste liquid collecting barrel (6) are sequentially connected by a solution conveying pipe; the liquid nitrogen side of the liquid nitrogen tank (3), the electromagnetic valve and the heat exchanger (7) are connected in sequence by a liquid nitrogen conveying pipe; the industrial control computer (8) is connected with a data acquisition control interface board (9), and the data acquisition control interface board (9) is connected with the multi-way switching valve (2), the electromagnetic valve, the electric heater (11) and the temperature sensor; the nozzle (15) is positioned at the bottom of the heat preservation box (14), and the stirring fan (10) is positioned at the top of the heat preservation box (14); freezing container (12) and waste liquid collecting vessel (6) between solution conveyer pipe on connect one section nonrust steel pipe (20), nonrust steel pipe (20) are outer around having electric heating wire and being located the solution import side of peristaltic pump (4), electric heating wire and a temperature controller link to each other.
2. The cryopreservation apparatus for medical biological materials as claimed in claim 1, wherein the number of the solution storage tanks is determined according to the cryopreservation scheme of biological materials, the number is 2-10, and the maximum path number of the multi-path switching valve (2) is 10.
3. The cryopreservation device of claim 2 wherein the incubator (14) is provided with a support (13), and the cryopreservation vessel (12) is placed on the support (13) and can be taken out of the support (13).
4. The cryopreservation device of claim 1, wherein the number of the temperature sensors is four, a first temperature sensor (16) is arranged at the solution side outlet of the heat exchanger (7), a second temperature sensor (17) is arranged on the solution outlet pipeline of the cryopreservation container (12), a third temperature sensor (18) is arranged in the incubator (14), and a fourth temperature sensor (19) is arranged on the liquid nitrogen delivery pipeline connected with the electromagnetic valve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922502059.1U CN211793997U (en) | 2019-12-31 | 2019-12-31 | Medical biomaterial low temperature save set |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922502059.1U CN211793997U (en) | 2019-12-31 | 2019-12-31 | Medical biomaterial low temperature save set |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211793997U true CN211793997U (en) | 2020-10-30 |
Family
ID=73041788
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201922502059.1U Expired - Fee Related CN211793997U (en) | 2019-12-31 | 2019-12-31 | Medical biomaterial low temperature save set |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211793997U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023072600A1 (en) * | 2021-10-26 | 2023-05-04 | Asymptote Ltd | Systems and methods for cryopreservation of biological material |
-
2019
- 2019-12-31 CN CN201922502059.1U patent/CN211793997U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023072600A1 (en) * | 2021-10-26 | 2023-05-04 | Asymptote Ltd | Systems and methods for cryopreservation of biological material |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN211793997U (en) | Medical biomaterial low temperature save set | |
| CN104677000B (en) | Liquid nitrogen cooling cryogenic device and implementation method for same | |
| JP6062054B2 (en) | Liquid feeding device and cell culture device using the same | |
| CN203320025U (en) | Fermentation tank capable of monitoring fermentation environment in centralized manner | |
| CN108717029A (en) | Low-temperature control system and control method for vacuum QCM | |
| CN108277334A (en) | A kind of deep cold treatment apparatus and method | |
| CN107960613B (en) | Pressure-regulating and temperature-controlling egg pickling equipment adopting liquid circulation and control method | |
| CN104986446A (en) | Automated biological low temperature preservation device | |
| CN102416205A (en) | Dry type plasma thawing instrument | |
| CN102039208A (en) | External circulating precise constant-temperature medium box and using method thereof | |
| CN111357738B (en) | Freezing and thawing control system for human embryo cryopreservation | |
| CN115363015A (en) | Automatic multi-style sample cryopreservation machine and using method | |
| CN212279636U (en) | Freezing and thawing control system for human embryo freezing | |
| CN204111762U (en) | A kind of low tempertaure storage device being applied to extensive animal cell culture and fermentable | |
| CN205981851U (en) | Clear up system in succession with adjustable pressure, flow | |
| CN215884977U (en) | Sample access device | |
| CN211451954U (en) | Movable liquid rapid temperature control device | |
| CN115199943B (en) | Liquid nitrogen liquid level control system | |
| CN117143722B (en) | Multichannel frozen cell synchronous thawing device and control method thereof | |
| CN216114906U (en) | Adjustable freezer | |
| CN207665849U (en) | A kind of quick deinsectization chamber system of port agricultural product | |
| CN206783300U (en) | A kind of paint filling equipment | |
| CN115226705B (en) | Cell cryopreservation and resuscitation integrated equipment | |
| CN103969288A (en) | Low-temperature-area thermal conductivity testing device | |
| CN114149899B (en) | Quick rewarming cryopreservation pipe device and use method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20201030 Termination date: 20211231 |