CN211793995U - Liquid nitrogen freezing storage box for sampling and sample separating of animal tissues - Google Patents
Liquid nitrogen freezing storage box for sampling and sample separating of animal tissues Download PDFInfo
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- CN211793995U CN211793995U CN201922010981.9U CN201922010981U CN211793995U CN 211793995 U CN211793995 U CN 211793995U CN 201922010981 U CN201922010981 U CN 201922010981U CN 211793995 U CN211793995 U CN 211793995U
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Abstract
The utility model discloses a liquid nitrogen cryopreservation box for sampling and sample separating of animal tissues, which is characterized by comprising a cover component and a box body matched with the cover component; the cover assembly sequentially comprises an outer cover, an inner cover and a heat insulation layer from top to bottom, a plurality of holes which are positioned in the cover assembly and penetrate through the outer cover, the inner cover and the heat insulation layer, and hole plugs which respectively seal the holes; sample storage devices are respectively accommodated in the holes. The liquid nitrogen cryopreservation box simplifies the processes of sampling, sample separation and cryopreservation of animal tissue samples, saves the using amount of liquid nitrogen, and ensures the quality of the samples in the preservation process.
Description
Technical Field
The utility model relates to a liquid nitrogen cryopreserved box that is used for animal tissue sampling branch appearance, be used for the sampling of animal tissue molecular appearance, divide appearance and cryopreserved very much.
Background
In recent years, with the rapid development of big data and informatization, relevant scientific research institutions and scientific research personnel all over the world play an irreplaceable important role in diagnosis, prevention and control of serious animal epidemic diseases. The accuracy and effectiveness of the laboratory monitoring results are influenced by factors such as experimental methods, experimental equipment, monitoring reagents, experimental operations and the like, and also by factors such as collection, transportation, storage and the like of samples.
The collection of animal disease samples is an important content in laboratory test work of animal epidemic diseases. How to collect animal disease material, collection time, whether the sample is properly processed, stored and transported, etc., are directly related to the accuracy and reliability of the test result, and have great influence on diagnosis of animal epidemic diseases, epidemiological investigation, immune monitoring, etc.
With the advent of the big data age, the data volume of various experiments has also increased dramatically. Therefore, in animal experiments, a large number of tissue samples are collected for experiments and detection, such as tissues of heart, liver, spleen, lung, kidney, pancreas, intestine (duodenum, jejunum, ileum, caecum, colon, rectum), brain (brain, cerebellum, delayed brain, etc.), spinal cord, muscle, nerve, etc., wherein the number of the samples can reach more than 20 per time, and the number of the samples can reach thousands, which causes a great problem of sample cryopreservation in scientific research experiments.
The conventional sample dividing cryopreservation method comprises the following steps: (1) sequentially putting collected animal tissue samples into the same large liquid nitrogen tank; (2) after sampling is finished, performing secondary sampling on the sample in the liquid nitrogen tank by using a liquid nitrogen lifting barrel, a small mesh bag or a cloth bag; (3) and respectively putting the samples after sample separation into foam boxes filled with liquid nitrogen for freezing storage. The method has the problems of large sample separation workload, serious waste of liquid nitrogen, overlong sample separation time, unfreezing of partial samples, deterioration of sample quality, influence on experimental results and the like. Meanwhile, the collected sample in the large liquid nitrogen tank is divided by using the liquid nitrogen lifting barrel and the small mesh bag, so that the large sample is not easy to be sampled, and the sample in the liquid nitrogen lifting barrel is easy to float out of the surface of liquid nitrogen in the lifting barrel, so that the quality of the sample is damaged; the liquid nitrogen bottle is classified by using a small mesh bag or a cloth bag, so that the mouth of the liquid nitrogen bottle is not easy to pull out; when the sample after being subjected to sample division is put into a foam box filled with liquid nitrogen for freezing storage, the liquid nitrogen in the foam box is volatile, so that liquid nitrogen waste and sample quality damage can be caused; and when the number of the foam boxes is large, the foam boxes easily occupy too much area of an experiment operation table, other experiment operations are hindered, and the safety is low.
Therefore, how to ensure that animal tissue samples with various quantities and varieties can be rapidly and efficiently collected and classified in a laboratory, and ensuring the preservation quality of the collected samples is a problem to be solved urgently by scientific researchers.
SUMMERY OF THE UTILITY MODEL
Therefore, the utility model provides an animal tissue sample sampling and divide small-size liquid nitrogen cryopreserved box of appearance realizes the sampling and the branch appearance to animal tissue sample simultaneously through adopting a plurality of little cryopreserved units to can guarantee the cryopreserved quality of sample better.
According to one aspect of the utility model, a liquid nitrogen cryopreservation box for sampling and sample separating of animal tissues is provided, which comprises a cover component and a box body matched with the cover component;
the cover assembly comprises an outer cover, an inner cover, a heat insulation layer positioned below the inner cover, a plurality of holes positioned in the cover assembly and penetrating through the outer cover, the inner cover and the heat insulation layer, and hole plugs for respectively sealing the holes;
sample storage devices are respectively accommodated in the holes
Preferably, the sample storage device is a pail having a through hole in the wall.
The box body comprises an inner box, an outer box and a heat-preservation interlayer positioned between the inner box and the outer box.
Two handles are arranged at two ends of the upper surface of the outer cover.
Handles are respectively arranged at the upper ends of the left side and the right side of the outer box.
The upper end of the lifting bucket is provided with a clamping edge and a handle.
The outer lid and the outer box are made of stainless steel plates.
The heat-insulating layer and the heat-insulating interlayer are made of foam materials.
The shape of the hole is one or more of a circle, a square and an ellipse.
According to the utility model discloses a method has following beneficial effect:
according to the utility model discloses a liquid nitrogen freezes deposits box is through the sample storage device that has the hole that adopts a plurality of small volumes in a big box that holds the liquid nitrogen, can conveniently from this, realize dividing the appearance simultaneously to the quantity of gathering, the numerous sample of variety to the quantity of liquid nitrogen can be practiced thrift to the storage device of little unit, and good sample freezes deposits the effect has been ensured to every storage device's sealed setting, has reduced that the liquid nitrogen volatilizees.
Drawings
FIG. 1 illustrates a lid assembly structure view of a liquid nitrogen cryopreservation cartridge according to an embodiment of the present invention;
FIG. 2 illustrates a box body structure diagram of a liquid nitrogen cryopreservation box according to an embodiment of the invention;
FIG. 3 illustrates a sample storage device according to an embodiment of the present invention;
fig. 4 illustrates a retractor for a sample storage device according to an embodiment of the present invention.
Detailed Description
Referring to fig. 1 to 4, the liquid nitrogen cryopreservation box for animal tissue sampling and sample separation provided by the invention comprises a cover component and a box body matched with the cover component.
Specifically, as shown in fig. 1, the cap assembly sequentially includes an outer cap 1, an inner cap 5, a heat insulating layer 6, a plurality of holes 2 penetrating through the outer cap 1, the inner cap 5, and the heat insulating layer 6, and hole plugs 3 respectively sealing the holes 2, from top to bottom.
Wherein the wells 2 each receive a sample storage device therein.
Specifically, the hole opening diameter in the inner cap is smaller than the hole opening diameter of the outer cap, while being smaller than the outer diameter of the sample storage device and larger than the inner diameter of the sample storage device.
Referring to fig. 1, the hole 2 includes a first hole section 2a in the outer cap 1 and a second hole section 2b in the inner cap 5, and the inner diameter of the first hole section 2a is larger than that of the second hole section 2 b.
The outer diameter of the upper port of the sample storage device (including the bead 12) is smaller than the inner diameter of the first well section 2a and larger than the inner diameter of the second well section 2b, while the outer diameter of the sample storage device is smaller than the inner diameter of the second well section 2 b. Thereby allowing the sample storage device to be inserted from the first well section 2a of the cover 1 and into the second well section 2 b. Meanwhile, due to the gap between the second hole inner section 2b and the sample storage device, liquid nitrogen can enter the sample storage device conveniently, and the animal tissue sample in the sample storage device can be frozen.
The cross-sectional shape of the holes 2 may be, for example, one or more of circular, square, and oval.
According to one embodiment, the outer cover 1 may be made of a stainless steel plate, for example a stainless steel plate of 2mm thickness. The inner cover 5 is made of a heat insulating material and a stainless steel plate.
The inner cap 5 and the outer cap 1 are connected by welding.
The materials of the heat insulating material in the inner cover 5 and the heat insulating layer 6 can be foam materials respectively.
Further, two handles 4 are arranged at two ends of the upper surface of the outer cover 1 for lifting the cover assembly.
The sample storage device is a pail with a through hole 14 formed in the pail wall 13. The through hole 14 is used for enabling liquid nitrogen in the box body to be immersed into the pail, and therefore freezing storage of samples in the pail is achieved.
As shown in fig. 2, the box body comprises an inner box 7, an outer box 9 and a heat-insulating interlayer 8 positioned between the inner box 7 and the outer box 9. Wherein the inner case 7 and the outer case 9 are made of stainless steel plates. For example, the inner case 7 and the outer case 9 may be made of a 1mm stainless steel plate and a 2mm stainless steel plate, respectively, and the insulating interlayer 8 may be a vacuum interlayer or made of an insulating material (e.g., a foam material) and may have a thickness of 3 cm.
Further, handles 10 are provided at upper end portions of both left and right sides of the outer box 8, respectively.
The pail, as further shown in figure 3, has a pail wall 13 provided with a plurality of through holes 14, a chime 12 at the upper end of the pail and a handle 11. When the pail is placed in the first hole section 2a of the hole 2 of the cover 1, the clamping edge 12 is clamped at the upper end edge of the second hole section 2b, so that the pail is inserted into the second hole section 2b of the hole and forms a sealing function. After the pail is placed in the hole 2, the hole 2 is plugged with a hole plug 3 for sealing. The material of the plug 3 may be foam or rubber.
From the above, the size of the outer cover 1 in the cover assembly is larger than that of the box body, and the size of the inner cover 5 is matched with that of the inner cavity of the box body, so that the box body can be sufficiently sealed.
According to one embodiment, the sample storage device may be pulled using the pull hook shown in FIG. 4.
Utilize the utility model discloses an above-mentioned liquid nitrogen freezes deposits box can put into the sample storage device of difference with the tissue sample of different grade type when gathering animal tissue sample to can carry out the sign on the stopple. Therefore, the sampling, classifying and freezing storage of the animal tissue sample can be realized efficiently and conveniently. Due to the fact that the independent unit sample storage devices are used, waste of liquid nitrogen can be reduced, the good sealing arrangement guarantees the freezing effect of the animal tissue samples, the quality of the animal tissue samples is guaranteed, and the scientific research experiment can be conducted efficiently and accurately.
Claims (10)
1. A liquid nitrogen cryopreservation box for sampling and separating animal tissues is characterized by comprising a cover assembly and a box body matched with the cover assembly;
the cover assembly sequentially comprises an outer cover, an inner cover and a heat insulation layer from top to bottom, a plurality of holes which are positioned in the cover assembly and penetrate through the outer cover, the inner cover and the heat insulation layer, and hole plugs which respectively seal the holes;
sample storage devices are respectively accommodated in the holes.
2. The liquid nitrogen cryopreservation cartridge for animal tissue sampling and aliquoting of claim 1, wherein the sample storage device is a pail provided with a through hole on a barrel wall.
3. The liquid nitrogen cryopreservation cartridge for animal tissue sampling and aliquoting of claim 1 wherein the cartridge body comprises an inner cartridge, an outer cartridge and an insulating barrier between the inner cartridge and the outer cartridge.
4. The liquid nitrogen cryopreservation box for animal tissue sampling and sample separation according to claim 1, wherein two handles are arranged at two ends of the upper surface of the outer cover.
5. The liquid nitrogen cryopreservation box for animal tissue sampling and sample separation as claimed in claim 3, wherein handles are respectively arranged at the upper ends of the left side and the right side of the outer box.
6. The liquid nitrogen cryopreservation box for animal tissue sampling and aliquoting of claim 2 wherein the upper end of the pail is provided with a clamping edge and a handle.
7. The liquid nitrogen cryopreservation cartridge for animal tissue sampling and aliquoting of claim 1 wherein the outer lid and the outer box are made of stainless steel plates.
8. The liquid nitrogen cryopreservation cartridge for animal tissue sampling and aliquoting of claim 3, wherein the heat preservation layer is made of foam material.
9. The liquid nitrogen cryopreservation cartridge for animal tissue sampling and aliquoting of claim 1, wherein the cross-sectional shape of the hole is one or more of circular, square and oval.
10. The liquid nitrogen cryopreservation box for animal tissue sampling and sample separation according to claim 3, wherein the thermal insulation interlayer is a vacuum layer or a foam layer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922010981.9U CN211793995U (en) | 2019-11-20 | 2019-11-20 | Liquid nitrogen freezing storage box for sampling and sample separating of animal tissues |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922010981.9U CN211793995U (en) | 2019-11-20 | 2019-11-20 | Liquid nitrogen freezing storage box for sampling and sample separating of animal tissues |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211793995U true CN211793995U (en) | 2020-10-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201922010981.9U Active CN211793995U (en) | 2019-11-20 | 2019-11-20 | Liquid nitrogen freezing storage box for sampling and sample separating of animal tissues |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211793995U (en) |
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2019
- 2019-11-20 CN CN201922010981.9U patent/CN211793995U/en active Active
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