CN211785565U - Enzyme-linked immunoassay device with membrane puncture function is sealed to consumptive material - Google Patents
Enzyme-linked immunoassay device with membrane puncture function is sealed to consumptive material Download PDFInfo
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- CN211785565U CN211785565U CN202020142612.5U CN202020142612U CN211785565U CN 211785565 U CN211785565 U CN 211785565U CN 202020142612 U CN202020142612 U CN 202020142612U CN 211785565 U CN211785565 U CN 211785565U
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- detection
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- sealing membrane
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- 238000002965 ELISA Methods 0.000 title claims abstract description 40
- 239000012528 membrane Substances 0.000 title claims abstract description 34
- 239000000463 material Substances 0.000 title claims description 8
- 238000001514 detection method Methods 0.000 claims abstract description 150
- 239000000523 sample Substances 0.000 claims abstract description 47
- 238000007789 sealing Methods 0.000 claims abstract description 34
- 238000002038 chemiluminescence detection Methods 0.000 claims abstract description 29
- 230000003287 optical effect Effects 0.000 claims description 36
- 238000006073 displacement reaction Methods 0.000 claims description 11
- 239000000126 substance Substances 0.000 claims description 2
- 238000010586 diagram Methods 0.000 description 10
- 238000003018 immunoassay Methods 0.000 description 7
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 description 3
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000002798 spectrophotometry method Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 239000000428 dust Substances 0.000 description 2
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 2
- 238000005375 photometry Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000004887 air purification Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
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Abstract
The utility model provides an ELISA detection device with consumable sealing membrane puncture function, which can perform chemiluminescence detection and photometric detection, can realize ELISA detection and chemiluminescence immunodetection on one device, and comprises a photometric detection unit, a chemiluminescent detection unit and a detection bin, wherein the photometric detection unit is provided with a detection groove for placing the detection bin; the chemiluminescence detection unit is positioned above the photometric detection unit, the chemiluminescence detection unit is provided with a probe and a movement mechanism connected with the probe, the probe is positioned above the detection groove, the movement mechanism drives the probe to move up and down, the chemiluminescence detection and the photometric detection of a solution to be detected can be easily and conveniently completed, the detection efficiency is improved, and the detection cost is reduced.
Description
Technical Field
The utility model belongs to the technical field of biochemical immunoassay, especially, relate to an enzyme-linked immunosorbent assay device with membrane puncture function is sealed to consumptive material.
Background
Biochemical immunoassay equipment is divided into various types of detection equipment such as an enzyme-linked immunoassay instrument and a chemiluminescent immunoassay instrument, wherein the enzyme-linked immunoassay instrument uses a spectrophotometry for detection, when light with different wavelengths is continuously irradiated to a sample solution with a certain concentration, absorption intensities corresponding to the different wavelengths can be obtained, and a qualitative and quantitative analysis method of substances is carried out by utilizing the curve; the chemiluminescence immunoassay instrument uses a chemiluminescence detection method, is a novel analysis method, has the advantages of extremely high sensitivity (for example, 2X 10' mol-L ATP can be measured by using the chemical reaction of fluorescein, fluorescein alcohol and Adenosine Triphosphate (ATP)), better selectivity, simplicity, high analysis speed (mostly within 1 min), wide linear range of several orders of magnitude and the like. Is widely applied in the fields of environment, life, medicine and the like.
Most of the existing instruments on the market mainly adopt a detection method, for example, an enzyme-linked immunosorbent assay (one type of enzyme-linked immunoassay) can only use a spectrophotometry, and cannot be used as a related detection item of chemiluminescence. The chemiluminescence detection instrument cannot perform spectrophotometry detection.
The chemiluminescence detection instrument has high requirement on the overall light shading of the instrument (because the sensitivity of a detection probe is high, the detection is greatly influenced by external light, and the detection probe can be damaged by the direct irradiation of the light), so that the enzyme-linked immunoassay analyzer and the chemiluminescence analyzer are not made into an integrated detection.
The consumables used by the two types of instruments are generally placed in an open mode or the sealing cover is opened before the instruments are placed in the open mode, dust pollutants in the air must be effectively controlled, and experiments of the type must be carried out in a laboratory with an air purification system.
SUMMERY OF THE UTILITY MODEL
The utility model discloses make the improvement to the problem that above-mentioned prior art exists, promptly the utility model provides an ELISA device with membrane puncture function is sealed to consumptive material has and can accomplish and realize ELISA and chemiluminescence immunodetection on an equipment.
In order to solve the technical problem, the utility model provides an enzyme-linked immunoassay device with consumable sealing membrane puncture function, which can perform chemiluminescence detection and photometric detection, can realize enzyme-linked immunoassay and chemiluminescence immunoassay on one device, and comprises a photometric detection unit, a chemiluminescence detection unit and a detection bin, wherein,
the photometric detection unit is provided with a detection groove, and the detection groove is used for placing the detection bin;
the chemiluminescence detection unit is positioned above the photometric detection unit and is provided with a probe and a movement mechanism connected with the probe, the probe is positioned above the detection groove, and the movement mechanism drives the probe to move up and down.
Preferably, a movement displacement platform is arranged below the photometric detection unit, a sliding rail is arranged on the movement displacement platform, and the photometric detection unit is arranged on the sliding rail in a sliding manner.
Preferably, a first stepping motor and a rotary-to-linear motion mechanism are arranged between the motion displacement platform and the photometric detection unit, and the first stepping motor drives the photometric detection unit to move on the slide rail.
Preferably, the photometric detection unit includes an optical lens group disposed on one side of the detection tank, the other side of the detection tank is provided with an optical detection receiving lens group, optical channels are disposed between the optical lens group and the detection tank, and between the optical detection receiving lens group and the detection tank, and the optical channels are provided with lenses.
Preferably, the optical lens group, the optical detection receiving lens group and the optical channel are arranged in two groups, and a 405nm light source and a 450nm light source are respectively adopted.
Preferably, the chemiluminescence detection unit comprises a second stepping motor, and the front end of the second stepping motor is connected with the probe and used for driving the probe to move up and down.
Preferably, the front end of the second stepping motor is connected with the probe through a lever mechanism, and the front end of the second stepping motor and the probe are respectively connected with two ends of the lever mechanism.
Preferably, a limiting part is arranged at the front end of the second stepping motor, and a limiting sensor is arranged in front of the limiting part.
Preferably, a sealing film is arranged at the upper end of the detection bin, and a film pricking puncture head is arranged at the front end of the probe.
Preferably, an elastic gasket is arranged on the probe to ensure the tightness of the detection cabin during photometric detection.
The utility model has the advantages that:
the utility model integrates chemiluminescence detection and photometric detection, can realize enzyme-linked immunoassay and chemiluminescence immunoassay on one device, and reduces the cost of detection equipment and detection procedures;
secondly, the sealing film on the detection bin is matched with the film pricking puncture head, so that the influence of dust on the experiment can be avoided to the greatest extent, and the detection precision is improved.
Drawings
FIG. 1 is a schematic structural diagram of an ELISA device with a consumable sealing membrane puncturing function according to an embodiment of the present invention;
FIG. 2 is a schematic structural diagram of a movement displacement platform of an ELISA device with a consumable sealing membrane puncturing function according to an embodiment of the present invention;
FIG. 3 is a schematic structural diagram of a photometric detection unit of an ELISA device with a consumable sealing membrane puncturing function according to an embodiment of the present invention;
FIG. 4 is a schematic structural diagram of a chemiluminescent detection unit of an ELISA device with a consumable sealing membrane puncturing function according to an embodiment of the present invention;
FIG. 5 is a schematic diagram of the structure of the chemiluminescence detection unit of the ELISA device with a consumable sealing membrane puncturing function according to the embodiment of the present invention, wherein the probe is located at the highest point;
FIG. 6 is a schematic structural diagram of a chemiluminescent detection unit of an ELISA device with a consumable sealing membrane puncturing function according to an embodiment of the present invention, in which a probe is located at the lowest point;
FIG. 7 is a schematic view of the local structure of the photometric detection unit of the ELISA device with the consumable sealing membrane puncturing function according to the embodiment of the present invention;
FIG. 8 is a schematic view of a partial structure of a chemiluminescent detection unit of an ELISA device with a consumable sealing membrane puncturing function according to an embodiment of the present invention before detection;
FIG. 9 is a schematic view of the local structure of the chemiluminescence detection unit of the enzyme-linked immunoassay device with the function of membrane sealing and puncturing for consumables according to the embodiment of the present invention;
FIG. 10 is a schematic structural view of the chemiluminescence detection of an ELISA device with a consumable sealing membrane puncturing function according to an embodiment of the present invention;
FIG. 11 is a schematic structural diagram of the ELISA device with the consumable sealing membrane puncturing function according to the embodiment of the present invention during photometric detection;
FIG. 12 is a schematic diagram of the detection principle of the photometric detection unit of the ELISA device with the function of puncturing the sealing film of the consumable in the embodiment of the present invention;
in the figure:
1. motion shift platform 2, photometric detection unit 3 and chemiluminescence detection unit
4. Bottom plate 5, slide rail 6, slider
7. Conveyor belt 8, first stepping motor 9 and first base
10. Optical lens group 11, detection tank 12 and optical detection receiving lens group
13. Circuit board mounting end face 14, second stepping motor 15 and lead screw
16. Lifting slide block 17, limiting part 18 and limiting sensor
19. Second base 20, lever 21, probe link
22. Light source 23, lens 24, and detection sensor
25. Probe 26, telescopic sleeve 27 and elastic washer
28. Puncture head 29 for puncturing membrane, tray 30 and optical channel
31. Solution 32 to be detected, sealing film 33, and emitted light
34. And (6) detecting the bin.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. It is to be understood that the embodiments described are only some embodiments of the invention, and not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
Exemplarily, as shown in fig. 1 to 4, the utility model provides an enzyme-linked immunoassay device with consumable sealing membrane puncturing function, which can perform chemiluminescence detection and photometric detection, and can realize enzyme-linked immunoassay and chemiluminescence immunoassay on one device, comprising a photometric detection unit 2, a chemiluminescence detection unit 3 and a detection bin 34, wherein,
the photometric detection unit 2 is provided with a detection groove 11, and the detection groove 11 is used for placing a detection bin 34;
the chemiluminescence detection unit 3 is positioned above the photometric detection unit 2, the chemiluminescence detection unit 3 is provided with a probe 25 and a movement mechanism connected with the probe 25, the probe 25 is positioned above the detection groove 11, and the movement mechanism drives the probe 25 to move up and down. Specifically, the solution 31 to be detected is placed in the detection bin 34, the detection bin 34 is placed on the detection tank 11, and the photometric detection unit 2 and the chemiluminescent detection unit 3 respectively detect the solution 31 to be detected in the detection bin 34.
Preferably, a moving displacement platform 1 is arranged below the photometric detection unit 2, a sliding rail 5 is arranged on the moving displacement platform 1, and the photometric detection unit 2 is slidably arranged on the sliding rail 5.
Preferably, a linear motor structure is arranged between the motion displacement platform 1 and the photometric detection unit 2, and specifically comprises a first stepping motor 8 and a rotary-to-linear motion mechanism, wherein the first stepping motor 8 drives the photometric detection unit 2 to move on the slide rail 5. Specifically, motion displacement platform 1 includes bottom plate 4, be provided with slide rail 5 on the bottom plate 4, the one end of slide rail 5 is provided with first step motor 8, be provided with slider 6 between photometry detecting element 2 and the slide rail 5, first step motor 8 is linear electric motor, first step motor 8 is connected with slider 6, specific accessible conveyer belt 7 or turbine worm mechanism or rack and pinion are connected, preferably, this application adopts conveyer belt 7 to connect, 7 position dentate conveyer belts of conveyer belt, be provided with the tooth with 7 case meshing of conveyer belt on the slider 6, first step motor 8 drives slider 6 and above photometry detecting element 2 through conveyer belt 7 and moves on slide rail 5.
Preferably, the photometric detection unit 2 includes a first base 9, the first base 9 is fixedly connected with a slider 6, a detection groove 11 is disposed on the first base 9, an optical lens group 10 is disposed on one side of the detection groove 11, an optical detection receiving lens group 12 is disposed on the other side of the detection groove 11, optical channels 30 are disposed between the optical lens group 10 and the detection groove 11, and between the optical detection receiving lens group 12 and the detection groove 11, and the optical channels 30 are all provided with lenses 23. The side of the first base on the side of the optical detection receiver set 12 is a circuit board mounting section 13 for mounting the related detection circuit. A light source is arranged in the optical lens group 10, light emitted by the light source sequentially passes through the light channel 30, the lens 23, the detection groove 11, the detection bin 34, the detection groove 11, the lens 23, the light channel 30 and the optical detection receiving lens group 12, and finally the light is focused on the detection sensor through the lens 23 to read a corresponding photometric value to complete detection.
In a preferred embodiment, as shown in fig. 3 and 12, two sets of optical lens assembly 10, optical detection receiving lens assembly 12 and optical channel 30 are provided, and respectively employ a 405nm light source and a 450nm light source. Preferably, the distance between the 405nm light source and the 450nm light source is greater than the width of the detection bin 34. Specifically, when performing photometric detection, the first stepper motor 8 drives the photometric detection unit 2 to move on the slide rail 5, so that the detection bin 34 is aligned to the optical channels 30 of the 405nm light source and the 450nm light source respectively for detection. After the photometric detection is completed, the first stepping motor 8 drives the photometric detection unit 2 to move on the slide rail 5, so that the detection bin is located in the middle of the two optical channels 30 for chemiluminescence detection, and the photometric detection unit 2 is prevented from interfering with chemiluminescence detection.
In a preferred embodiment, as shown in fig. 4 to 6, the chemiluminescence detecting unit 3 comprises a second stepping motor 14, and the front end of the second stepping motor 14 is connected with the probe 25 for driving the probe 25 to move up and down. The probe 25 can be extended into the interior of the test chamber 34 for chemiluminescence test. Specifically, the two-step motor 14 is a vertically disposed linear motor, and the output end of the two-step motor is connected to the probe 25 and drives the probe to move in the vertical direction.
In a further preferable scheme, the front end of the second stepping motor 14 is connected with the probe 25 through a lever mechanism, and the front end of the second stepping motor 14 and the probe 25 are respectively connected with two ends of a lever 20 of the lever mechanism. Specifically, a screw rod 15 is arranged at the front end of the second stepping motor 14, a lifting slider 16 is arranged at the front end of the screw rod 15, and the lifting slider 16 is connected with one end of a lever 20 of the lever mechanism.
In a further preferable scheme, a limiting member 17 is arranged at the front end of the second stepping motor 14, and a limiting sensor 18 is arranged in front of the limiting member 17. The height of the probe 25 is precisely controlled.
In a preferred embodiment, as shown in fig. 7 to 11, a sealing film 32 is disposed at the upper end of the detection chamber 34, and a puncture head 28 for puncturing a film is disposed at the front end of the probe 25. The sealing film 32 reduces the influence of external factors on the solution to be detected in the detection chamber 34.
In a further preferred embodiment, the probe 25 is provided with an elastic gasket 27 for ensuring the tightness of the test chamber 34 during photometric tests.
In a further preferred embodiment, a tray 29 is disposed above the test slot 11 for placing and fixing the test chamber 34. Specifically, the detection chamber is placed on the tray 29, and the corresponding detection test is completed by changing the positions of the photometric detection unit 2 and the chemiluminescent detection unit 3 relative to the tray 29.
As shown in fig. 5-6, the left and right diagrams are respectively a schematic structural diagram of the chemiluminescence detection unit 3 in an open state and a closed state, a telescopic sleeve 26 is arranged on the probe 25, the telescopic sleeve 26 is driven by a probe connecting rod 21 connected with the probe to move up and down, the interior of the membrane puncturing head 28 is hollow, the membrane puncturing head 28 is communicated with the chemiluminescence detection sensor to collect optical signals when the device is in the open state, and when the device is in the closed state, the optical path of the chemiluminescence detection sensor can be completely blocked, so that the sensor is protected from being damaged by strong light.
As shown in fig. 7 to 11, the solution 31 to be tested is placed in the test chamber 34, and the test chamber 34 is placed on the tray 29. The tray 29 functions to move the test chamber 34 into the test slot 11 of the mechanism shown in this application and to remain stationary.
The tray 29 mainly plays a role in fixing and positioning. The detection bin 34 is not internally filled with any liquid before being used, when the detection bin is required to be used, the telescopic sleeve 26 is pressed downwards and the sealing film 32 on the detection bin 34 is punctured by the puncturing head 28, the tray 29 is connected to drive the detection bin 34 to temporarily move to the outer side of the device for liquid adding treatment and other steps required by an experiment, and the tray 29 drives the detection bin 34 to move back to the device provided by the application after liquid adding is finished.
Wherein the telescoping sheath 26 remains closed during photometric detection. Light emitted by the light source 22 passes through the solution to be detected 31 through the light channel and enters the light hole of the receiving end, and is identified by the detection sensor, so that the detection operation is completed.
Through the technical scheme, the enzyme-linked immunoassay device with the consumable sealing membrane puncturing function can easily and conveniently complete chemiluminescence detection and photometric detection on the solution 31 to be detected, improves the detection efficiency and reduces the detection cost.
The embodiments of the present invention have been described in detail, but the description is only for the preferred embodiments of the present invention and should not be construed as limiting the scope of the present invention. All the equivalent changes and improvements made according to the application scope of the present invention should still fall within the patent coverage of the present invention.
Claims (10)
1. An enzyme-linked immunoassay device with consumable sealing membrane puncture function is characterized by comprising a photometric detection unit, a chemiluminescence detection unit and a detection bin, wherein,
the photometric detection unit is provided with a detection groove, and the detection groove is used for placing the detection bin;
the chemiluminescence detection unit is positioned above the photometric detection unit and is provided with a probe and a movement mechanism connected with the probe, the probe is positioned above the detection groove, and the movement mechanism drives the probe to move up and down.
2. The ELISA device for detecting the puncture of the sealing membrane of the consumable material according to claim 1, wherein a moving displacement platform is arranged below the photometric detection unit, a slide rail is arranged on the moving displacement platform, and the photometric detection unit is slidably arranged on the slide rail.
3. The ELISA device for detecting the puncture of the sealing membrane of the consumable material as claimed in claim 2, wherein a first stepping motor and a rotary-to-linear motion mechanism are disposed between the moving displacement platform and the photometric detection unit, and the first stepping motor drives the photometric detection unit to move on the slide rail.
4. The ELISA device for detecting the puncture of the sealing membrane of the consumable material as claimed in claim 1, wherein the photometric detection unit comprises an optical lens set disposed on one side of the detection slot, an optical detection receiving lens set disposed on the other side of the detection slot, and optical channels disposed between the optical lens set and the detection slot, and between the optical detection receiving lens set and the detection slot, and provided with lenses.
5. The ELISA device for detecting the puncture of the sealing membrane of the consumable material as claimed in claim 4, wherein the optical lens set, the optical detection receiving lens set and the optical channel are arranged in two groups, respectively using a 405nm light source and a 450nm light source.
6. The ELISA device for detecting the puncture of the sealing membrane of the consumable substance according to claim 1, wherein the chemiluminescence detection unit comprises a second stepping motor, and the front end of the second stepping motor is connected with the probe for driving the probe to move up and down.
7. The ELISA device for detecting the presence of the consumable sealing membrane puncture function according to claim 6 wherein the front end of the second stepping motor is connected to the probe through a lever mechanism, and the front end of the second stepping motor and the probe are respectively connected to two ends of the lever mechanism.
8. The ELISA device for detecting the presence of the consumable sealing membrane puncture function of claim 7 wherein the second stepper motor is provided with a position-limiting element at the front end, and a position-limiting sensor is provided in front of the position-limiting element.
9. The ELISA device for detecting the presence of a consumable sealing membrane puncture function according to any one of claims 1 to 8, wherein a sealing membrane is disposed at the upper end of the detection chamber, and a membrane puncture head is disposed at the front end of the probe.
10. The ELISA device for detecting the presence of a consumable sealing membrane puncture function of claim 9 wherein the probe is provided with an elastic gasket for ensuring the tightness of the detection chamber during photometric detection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020142612.5U CN211785565U (en) | 2020-01-22 | 2020-01-22 | Enzyme-linked immunoassay device with membrane puncture function is sealed to consumptive material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020142612.5U CN211785565U (en) | 2020-01-22 | 2020-01-22 | Enzyme-linked immunoassay device with membrane puncture function is sealed to consumptive material |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211785565U true CN211785565U (en) | 2020-10-27 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020142612.5U Active CN211785565U (en) | 2020-01-22 | 2020-01-22 | Enzyme-linked immunoassay device with membrane puncture function is sealed to consumptive material |
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| Country | Link |
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| CN (1) | CN211785565U (en) |
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