CN211785523U - Chloroform fumigation device for measuring soil microbial biomass carbon - Google Patents
Chloroform fumigation device for measuring soil microbial biomass carbon Download PDFInfo
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- CN211785523U CN211785523U CN202020011738.9U CN202020011738U CN211785523U CN 211785523 U CN211785523 U CN 211785523U CN 202020011738 U CN202020011738 U CN 202020011738U CN 211785523 U CN211785523 U CN 211785523U
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- fumigation
- chloroform
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Abstract
The utility model relates to a chloroform fumigation apparatus for determining soil microorganism biomass carbon, including fumigation jar, fumigation room, chloroform heating chamber, and be provided with soil sample fumigation groove and agitating unit in the fumigation room, soil sample fumigation groove and chloroform heating chamber are through setting up a plurality of air inlet columns on the baffle intercommunication, agitating unit includes first agitator and second agitator, fumigation tank top is provided with air pump, gas transmission pipeline, blast pipe, is provided with first valve on the blast pipe, be provided with the second valve on the gas transmission pipeline; the utility model discloses in through the first agitator and the second agitator that set up, stir soil, enlarged the area of contact of soil and chloroform, secondly, through the integrated design of air pump, pipe, rectangle connecting pipe for chloroform stifling gas used repeatedly, simultaneously through the feedback control return circuit that temperature sensor, main control system and electric heating board are constituteed, can automatic control stifling temperature to default.
Description
Technical Field
The utility model relates to a soil microorganism biomass carbon nitrogen determination especially relates to a chloroform fumigation apparatus for determining soil microorganism biomass carbon.
Background
The soil microbial biomass refers to the total amount of live microorganisms with the volume less than 5 mu m3-10 mu m3 in soil, the nutrients of the total microorganisms account for corresponding elements in the soil in a small proportion, but the total amount is significant in evaluating the effect of the total microorganisms on the crop nutrition. Because it is very active and can rapidly participate in nutrient circulation, both as a driving force for the nutrient circulation process and as a "converter" of organic substances entering the soil, and as a "source" and "sink" for the internal supply mechanism of soil energy and nutrients, in particular C, N, P, S (ref 1: Jenkinson DS, Ladd J N, Microbial biomass in soil: measurements and turnover, Soil biochemistry, 1981, (5): 415-.
The proportion of microbial biomass carbon in the total soil carbon is small, generally only 1% -4% of the total soil organic carbon, but for the effective soil nutrients, the microbial biomass carbon is a great source and stock, and is the most active part in soil organic matters, can reflect the effective conditions and biological activity of the soil nutrients, can reflect the number of soil microorganisms to a great extent, and is a parameter index for evaluating the microbial biomass and activity. The microbial biomass carbon conversion is rapid, can reflect the change of soil organic matters before the change of the total carbon of the soil is detected, and is a more sensitive soil quality index (document 2: admiralty, haumingde, the influence of long-term rotation fertilization on the carbon and nitrogen of soil microorganisms, and the water and soil conservation research, 2003, 10 (1): 85-87). Are commonly used to estimate soil biological status and are critical to evaluating soil quality status.
The method comprises the steps of fumigating a soil sample by chloroform, cracking dead cells of soil microorganisms to release microorganism biomass carbon, extracting the soil by using a certain volume of LK2SO4 solution, measuring the content of the microorganism biomass carbon by using an organic carbon automatic analyzer, and measuring the difference and the conversion coefficient of organic carbon according to the fumigated soil and un-fumigated soil SO as to calculate the soil microorganism biomass carbon. The device that current fumigation was used generally adopts glass material, and the gas tightness is poor easily to damage, and when fumigating moreover, in the whole device of chloroform steam, not to fumigating soil, the fumigation effect is poor and efficient.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a chloroform fumigation apparatus for determining soil microorganism biomass carbon has solved the problem that exists among the above-mentioned background art.
The utility model adopts the technical proposal that:
a chloroform fumigation device for measuring soil microbial biomass carbon comprises a fumigation tank, wherein supporting seats are arranged at four corners of the bottom of the fumigation tank, and the chloroform fumigation device is characterized in that the interior of the fumigation tank is divided into an upper fumigation chamber and a lower chloroform heating chamber by a partition plate, a first temperature sensor is arranged on the side wall of the interior of the chloroform heating chamber, a first heating plate is arranged at the bottom of the chloroform heating chamber, the first heating plate is connected with the output end of a first heater arranged at the bottom of the fumigation tank, and the side wall of the chloroform heating chamber is connected with a feeding pipeline;
the soil sample fumigating chamber is internally provided with a soil sample fumigating groove and a stirring device, the soil sample fumigating groove is communicated with the chloroform heating chamber through a plurality of air inlet columns arranged on the partition plate, the stirring device comprises a first stirrer, a second stirrer and a stirring motor respectively connected with the first stirrer and the second stirrer, the first stirrer and the second stirrer are oppositely arranged in the soil sample fumigating groove, the top of the fumigating tank is provided with an air pump, the input end of the air pump is communicated with the fumigating chamber through an air outlet pipeline, the output end of the air pump is connected with an air pipeline, the air pipeline is connected with a branch pipeline, the branch pipeline is an exhaust pipe, the exhaust pipe is provided with a first valve, one end of the air pipeline, far away from the air pump, is positioned below the exhaust pipe and is provided with a second valve, and the output end of the second valve is communicated with a rectangular connecting pipe through a guide pipe, the rectangular connecting pipe is arranged in the fumigation chamber and is positioned above the soil sample fumigation groove, and a plurality of gas transmission spray heads are arranged at the bottom of the rectangular connecting pipe;
still be provided with the main control system on the fumigation jar, the main control system is connected with air pump, agitator motor, first heater and first temperature sensor electricity, be provided with display screen and operating keyboard on the main control system.
Preferably, the left side wall and the right side wall of the fumigation chamber are respectively provided with a second heating plate, and the second heating plates are connected with the output ends of second heaters arranged on the left side wall and the right side wall of the fumigation tank; and a second temperature sensor is also arranged in the fumigation chamber, and the second heater and the temperature sensor are electrically connected with the control host.
Preferably, the middle position of one side of the fumigation tank close to the control host is provided with a material placing bin door, the material placing bin door is provided with a locking buckle, the locking buckle and a locking member arranged on the fumigation tank work in a matched mode, and a sealing structure is arranged inside the material placing bin door.
Preferably, the length of the rectangular connecting pipe is greater than that of the soil sample fumigation groove, and 8 groups of gas transmission spray heads are arranged on the rectangular connecting pipe.
Preferably, the end face of the air inlet column close to the soil sample fumigation groove is provided with a mesh screen, and the number of the air inlet columns is 6.
Preferably, the first stirrer and the second stirrer have the same structure, wherein the first stirrer comprises a first vertical stirring sheet, a second vertical stirring sheet and a mounting disc, and a main shaft of the stirring motor is fixedly connected with the mounting disc.
Preferably, the control host is PLC S7-200CN in model, the control host is fixed on the tank body of the fumigation tank through screws, the display screen is glued on the control host, and the operation keyboard is embedded on the control host.
Compared with the prior art, the beneficial effects of the utility model are that: the utility model discloses in through the first agitator and the second agitator that set up, stir soil, the area of contact of soil and chloroform has been enlarged, upset about the soil microorganism is carried out to the first perpendicular stirring piece of cooperation and the perpendicular stirring piece of second, the area of soil microorganism and the gaseous contact of chloroform has further been enlarged, and then the stifling efficiency of improvement, secondly, through the air pump, the pipe, the integrated design of rectangle connecting pipe, make the gaseous used repeatedly of chloroform fumigation, stifling time has further been shortened, simultaneously through temperature sensor, the feedback control return circuit that control host computer and electric heating board are constituteed, can automatic control fumigation temperature to default.
Drawings
Fig. 1 is a schematic structural diagram of a chloroform fumigation apparatus for measuring soil microbial biomass carbon according to an embodiment of the present invention;
fig. 2 is a cross-sectional view of a chloroform fumigation apparatus for measuring carbon content in soil microorganisms according to an embodiment of the present invention;
fig. 3 is an internal view of a door of a hopper in a chloroform fumigation apparatus for measuring carbon content in soil microorganisms according to an embodiment of the present invention;
fig. 4 is a structural diagram of a rectangular connecting pipe in a chloroform fumigation apparatus for measuring soil microbial biomass carbon according to an embodiment of the present invention.
Wherein, 1-a fumigation tank; 2, an air pump; 3-a gas pipeline; 4-an exhaust pipe; 5-a first valve; 6-a second valve; 7-a discharge bin gate; 8-a first heater; 9-a support seat; 10-a feed line; 11-a control host; 12-operating a keyboard; 12-a display screen; 14-a second heater; 15-a locking member; 16-a locking buckle; 17-a fumigation chamber; 18-rectangular connecting tubes; 19-a first vertical stirring blade; 20-mounting a disc; 21-a soil sample fumigation tank; 22-a second vertical stirring blade; 23-a second temperature sensor; 24-chloroform heating chamber; 25-a first heating plate; 26-an air inlet column; 27-a separator; 28-stirring motor; 29-a second heating plate; 30-a gas transmission spray head; 31-a second temperature sensor; 32-sealing structure.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
Referring to fig. 1 and 2, the utility model discloses a chloroform fumigation apparatus for determining soil microorganism biomass carbon, including fumigation tank 1, supporting seats 9 are installed at four corners of the bottom of the fumigation tank, the interior of fumigation tank 1 is divided into an upper fumigation chamber and a lower chloroform heating chamber 24 by partition plate 27, a first temperature sensor 23 is arranged on the side wall of the interior of chloroform heating chamber 24, a first heating plate 25 is arranged at the bottom of the first temperature sensor, the first heating plate 25 is connected with the output end of a first heater 8 arranged at the bottom of fumigation tank 1, and the side wall of chloroform heating chamber 24 is connected with a feeding pipeline 10; the soil sample fumigating chamber is internally provided with a soil sample fumigating groove 21 and a stirring device, the soil sample fumigating groove 21 is communicated with a chloroform heating chamber 24 through a plurality of air inlet columns 26 arranged on a partition plate 27, the stirring device comprises a first stirrer, a second stirrer and a stirring motor 28 respectively connected with the first stirrer and the second stirrer, the first stirrer and the second stirrer are oppositely arranged in the soil sample fumigating groove 21, the top of the fumigating tank 1 is provided with an air pump 2, the input end of the air pump 2 is communicated with the fumigating chamber through an air outlet pipeline, the output end of the air pump 2 is connected with an air pipeline 3, the air pipeline 3 is connected with a branch pipeline which is an exhaust pipe 4, the exhaust pipe 4 is provided with a first valve 5, one end of the air pipeline 3 far away from the air pump 2 and a second valve 6 is arranged below the exhaust pipe, the output end of the second valve 6 is communicated with a rectangular connecting pipe 18 through a conduit, the rectangular connecting pipe 18 is arranged in the fumigation chamber and is positioned above the soil sample fumigation groove 21, the bottom of the rectangular connecting pipe 18 is provided with a plurality of gas transmission spray nozzles 30, the left and right side walls in the fumigation chamber are respectively provided with a second heating plate 29, and the second heating plates 29 are connected with the output ends of the second heaters 14 arranged on the left and right side walls of the fumigation tank 1; and a second temperature sensor 31 is also arranged in the fumigation chamber.
Referring to fig. 3, as a further improvement of the present invention, a material placing door 7 is disposed at a middle position of one side of the fumigation tank 1 close to the control host 11, a locking buckle 16 is disposed on the material placing door 7, the locking buckle 16 cooperates with a locking member 15 disposed on the fumigation tank 1, and a sealing structure 32 is disposed inside the material placing door 7; fresh soil is introduced into the soil sample fumigation groove 21 through the emptying bin opening 7, and the sealing structure 32 arranged inside the emptying bin door 7 is used for ensuring the sealing performance of the fumigation chamber, wherein the sealing structure preferably uses a sealing ring, but is not limited to the sealing ring, and other structures capable of achieving the same sealing effect can also be used.
In this embodiment, the first stirrer and the second stirrer have the same structure, wherein the first stirrer comprises a first vertical stirring blade 19, a second vertical stirring blade 22 and a mounting plate 20, and a main shaft of the stirring motor 28 is fixedly connected with the mounting plate 20; the first vertical stirring piece 19 and the second vertical stirring piece 22 on the first stirrer are opposite to the rotating direction of the first vertical stirring piece 19 and the second vertical stirring piece 22 on the second stirrer, so that the stirring and mixing quality of the soil is improved, the soil and the chloroform gas can be conveniently and fully mixed and reacted, and the fumigation efficiency is further improved.
In this embodiment, a mesh screen is disposed on the end surface of the air inlet column 26 close to the soil sample fumigating groove 21, and the number of the air inlet columns 26 is 6. Still be provided with main control system 11 on the fumigation jar 1, main control system 11' S model is PLC S7-200CN, main control system 11 passes through the fix with screw on the jar body, display screen 13 splices on main control system 11, operating keyboard 12 is embedded on main control system 11, main control system 11 is connected with air pump 2, agitator motor 28, first heater 8, second heater 14 and first temperature sensor 23 and second temperature sensor 31 electricity. The first temperature sensor 23 and the second temperature sensor 31 are operated at a refresh frequency of not less than 50HZ, and are preferably temperature sensors of model WJ200 DA.
Referring to fig. 4, in order to further provide the using function of the fumigation apparatus for soil microorganism biomass carbon, the length of the rectangular connecting pipe 18 arranged on the guide pipe is longer than that of the soil sample fumigation groove 21, and the number of the gas transmission nozzles 30 arranged on the rectangular connecting pipe is 8, so that the chloroform gas absorbed by the gas pump 2 is output through the gas transmission nozzles 30, and the utilization rate of the chloroform gas is improved.
The working principle of the device is as follows: before use, the device is powered on, when soil and chloroform need to be placed, soil microorganisms are placed into the soil sample fumigation groove 21 through the material placing bin door 7, and after material adding is finished, the material placing bin door 7 is locked through the cooperation of the locking piece 15 and the lock catch 16; then chloroform and ethanol are added through a feeding pipeline 10, when fumigation is to be carried out, firstly, an air pump 2 is opened through a control host to vacuumize the interior of a fumigation tank 1, a first valve 5 is opened, a second valve 6 is closed, gas is discharged, then, a first heater 8 is opened through the control host 11, the first heater 8 heats chloroform through a first heating plate 25, the boiled chloroform gas enters soil from the bottom of a soil sample fumigation tank 21 through an air inlet column 26, at the moment, a stirring motor 28 on two side walls of the soil sample fumigation tank 21 is opened through the control host 11, so that a first stirrer and a second stirrer are driven to overturn soil microorganisms in the interior to improve the contact area of the chloroform gas and the soil, then, the first valve 5 is closed, the second valve 6 is opened, the air pump 2 is opened through the control host 11, the chloroform gas in the interior is pumped out and then is conveyed into a rectangular connecting pipe 18, thereby spraying the chloroform into the soil through the gas transmission nozzle 30 and improving the utilization rate of the chloroform gas. The whole fumigation chamber is placed under the dark condition to fumigate soil for 24 hours, after the fumigation is finished, the fumigation tank is vacuumized by opening the first valve 5 and the second valve 6, then the first valve 5 and the second valve 6 which are opened during the vacuumization are closed, the air pump 2 is started, the first valve 5 is opened, the second valve 6 is closed, and the soil is vacuumized until the soil has no chloroform smell.
The first temperature sensor 23 and the second temperature sensor 21 are used for respectively acquiring temperature information of fumigation gas in the fumigation chamber and the chloroform heating chamber in the tank in real time and transmitting the temperature information back to the control host 11, the control host 11 compares the temperature information with a preset fumigation temperature value, and the temperature information is controlled to be the preset fumigation temperature value by adjusting the air filter of the heater. Secondly, just after stifling, can open the second heater, the second heater heats inside through the second hot plate, keeps the inside constant temperature of device.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the above embodiments, and that the foregoing embodiments and descriptions are provided only to illustrate the principles of the present invention without departing from the spirit and scope of the present invention.
Claims (7)
1. A chloroform fumigation device for measuring soil microbial biomass carbon comprises a fumigation tank, wherein supporting seats are arranged at four corners of the bottom of the fumigation tank, and the chloroform fumigation device is characterized in that the interior of the fumigation tank is divided into an upper fumigation chamber and a lower chloroform heating chamber by a partition plate, a first temperature sensor is arranged on the side wall of the interior of the chloroform heating chamber, a first heating plate is arranged at the bottom of the chloroform heating chamber, the first heating plate is connected with the output end of a first heater arranged at the bottom of the fumigation tank, and the side wall of the chloroform heating chamber is connected with a feeding pipeline;
the soil sample fumigating chamber is internally provided with a soil sample fumigating groove and a stirring device, the soil sample fumigating groove is communicated with the chloroform heating chamber through a plurality of air inlet columns arranged on the partition plate, the stirring device comprises a first stirrer, a second stirrer and a stirring motor respectively connected with the first stirrer and the second stirrer, the first stirrer and the second stirrer are oppositely arranged in the soil sample fumigating groove, the top of the fumigating tank is provided with an air pump, the input end of the air pump is communicated with the fumigating chamber through an air outlet pipeline, the output end of the air pump is connected with an air pipeline, the air pipeline is connected with a branch pipeline, the branch pipeline is an exhaust pipe, the exhaust pipe is provided with a first valve, one end of the air pipeline, far away from the air pump, is positioned below the exhaust pipe and is provided with a second valve, and the output end of the second valve is communicated with a rectangular connecting pipe through a guide pipe, the rectangular connecting pipe is arranged in the fumigation chamber and is positioned above the soil sample fumigation groove, and a plurality of gas transmission spray heads are arranged at the bottom of the rectangular connecting pipe;
still be provided with the main control system on the fumigation jar, the main control system is connected with air pump, agitator motor, first heater and first temperature sensor electricity, be provided with display screen and operating keyboard on the main control system.
2. The chloroform fumigation device for measuring soil microbial biomass carbon as claimed in claim 1, wherein second heating plates are respectively arranged on the left and right side walls in the fumigation chamber, and the second heating plates are connected with the output ends of second heaters arranged on the left and right side walls of the fumigation tank; and a second temperature sensor is also arranged in the fumigation chamber, and the second heater and the temperature sensor are electrically connected with the control host.
3. The chloroform fumigation device for measuring soil microbial biomass carbon as claimed in claim 1, wherein a material discharge bin door is arranged at the middle position of one side of the fumigation tank close to the control host, a locking buckle is arranged on the material discharge bin door, the locking buckle is matched with a locking piece arranged on the fumigation tank, and a sealing structure is arranged inside the material discharge bin door.
4. The chloroform fumigation device for measuring soil microbial biomass carbon as claimed in claim 1, wherein the length of said rectangular connecting pipe is longer than that of soil sample fumigation tank, and its upper portion is equipped with 8 groups of gas-conveying nozzles.
5. The chloroform fumigation device for measuring soil microbial biomass carbon as claimed in claim 1, wherein a mesh screen is arranged on the end face of the air inlet column close to the soil sample fumigation groove, and the number of the air inlet columns is 6.
6. The chloroform fumigation device for measuring soil microbial biomass carbon as claimed in claim 1, wherein the first stirrer and the second stirrer are identical in structure, wherein the first stirrer comprises a first vertical stirring blade, a second vertical stirring blade and a mounting plate, and a main shaft of the stirring motor is fixedly connected with the mounting plate.
7. The chloroform fumigation device for determining soil microbial biomass carbon as claimed in claim 1, wherein the model of said control host is PLC S7-200CN, said control host is fixed on the tank body of said fumigation tank by screws, said display screen is glued on said control host, and said operation keyboard is embedded in said control host.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020011738.9U CN211785523U (en) | 2020-01-04 | 2020-01-04 | Chloroform fumigation device for measuring soil microbial biomass carbon |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020011738.9U CN211785523U (en) | 2020-01-04 | 2020-01-04 | Chloroform fumigation device for measuring soil microbial biomass carbon |
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| CN211785523U true CN211785523U (en) | 2020-10-27 |
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| CN202020011738.9U Expired - Fee Related CN211785523U (en) | 2020-01-04 | 2020-01-04 | Chloroform fumigation device for measuring soil microbial biomass carbon |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112567916A (en) * | 2021-01-07 | 2021-03-30 | 郑祥瑞 | Reagent method fumigation system for soil improvement |
| CN113000582A (en) * | 2021-02-25 | 2021-06-22 | 永清环保股份有限公司 | System for volatile organic compounds pollutes soil is handled in normal atmospheric temperature desorption |
| CN114226429A (en) * | 2021-12-30 | 2022-03-25 | 湖南博一环保科技有限公司 | Chemical remediation method for organic contaminated soil |
| CN114354306A (en) * | 2021-12-14 | 2022-04-15 | 商丘师范学院 | Soil microorganism fumigation device |
-
2020
- 2020-01-04 CN CN202020011738.9U patent/CN211785523U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112567916A (en) * | 2021-01-07 | 2021-03-30 | 郑祥瑞 | Reagent method fumigation system for soil improvement |
| CN113000582A (en) * | 2021-02-25 | 2021-06-22 | 永清环保股份有限公司 | System for volatile organic compounds pollutes soil is handled in normal atmospheric temperature desorption |
| CN114354306A (en) * | 2021-12-14 | 2022-04-15 | 商丘师范学院 | Soil microorganism fumigation device |
| CN114354306B (en) * | 2021-12-14 | 2024-01-26 | 商丘师范学院 | Soil microorganism fumigation device |
| CN114226429A (en) * | 2021-12-30 | 2022-03-25 | 湖南博一环保科技有限公司 | Chemical remediation method for organic contaminated soil |
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| GR01 | Patent grant | ||
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| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20201218 Address after: 210036 15th floor, No.70 Qingjiang South Road, Gulou District, Nanjing City, Jiangsu Province Patentee after: Nanjing JuShui Technology Co.,Ltd. Address before: Longpan road Xuanwu District of Nanjing city of Jiangsu Province, No. 159 210037 Patentee before: NANJING FORESTRY University |
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
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Granted publication date: 20201027 Termination date: 20220104 |