CN211741195U - Simple stable isotope gas marking device - Google Patents
Simple stable isotope gas marking device Download PDFInfo
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- CN211741195U CN211741195U CN201921927737.2U CN201921927737U CN211741195U CN 211741195 U CN211741195 U CN 211741195U CN 201921927737 U CN201921927737 U CN 201921927737U CN 211741195 U CN211741195 U CN 211741195U
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- stable isotope
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Abstract
The utility model relates to a technical field of isotope marking device, more specifically relates to a simple and easy gaseous mark device of stable isotope. A simple stable isotope gas marking device comprises a marking box and a cover arranged at the upper part of the marking box, wherein a cultivation mechanism, a beaker, a water pump and a triangular flask for loading lactic acid are arranged in the marking box; the beaker, the water pump and the triangular flask are connected through pipelines, and the water pump is connected with an external power supply; the cultivation mechanism plants to be marked. The utility model discloses a device need not extra light source, and the device is simple effective, and the volume size can design as required in a flexible way, the incasement13CO2The gas concentration can be adjusted according to the size of the box body to add Ba13CO3The dosage of the powder and the lactic acid is flexibly controlled, and the fan ensures13CO2The gas distribution is even, convenient operation, and the material is comparatively common, and the cost of manufacture is low.
Description
Technical Field
The utility model relates to a technical field of isotope marking device, more specifically relates to a simple and easy gaseous mark device of stable isotope.
Background
Isotopes are two or more atoms of the same chemical element having the same atomic number, occupying the same position in the periodic table of the elements, having almost the same chemical properties but different atomic mass or mass number, and thus differing in their mass spectral behavior, radioactive transitions and physical properties (e.g. diffusivity in the gaseous state). Isotopes are classified into two types, namely radioactive isotopes and stable isotopes. The radioactive isotope is the first tracer to be applied in practice, and the stable isotope is gradually raised in scientific research and rapidly developed with the advantages of safety, stability, convenience and the like in later period along with the development and maturation of mass spectrum technology.
The isotope feeding to the plants can conveniently research the absorption, transportation and metabolic processes of a certain specific substance in the plants. For example, O production during photosynthesis in plants2The source of (A) and the study of the carbon fixation and transmission process in photosynthesis are not separated from the contribution of isotope labeling.
Jenkinson designed the first use in 196014The device for marking the plants by the C radioactive isotopes is used for culturing the marked plants in a water culture mode in a closed space, and later researchers further introduce a device which can be used for marking the plants growing in different growing media and obtain good marking effect, but the device has the advantages that14C is a radioactive isotope which is harmful to the environment and therefore remains after labeling14C needs to be recovered, and the operation is relatively complex. And, currently prevalent CO2The marking devices have the problems of being heavy, relatively high in price, relatively large in size, relatively complicated in operation and the like, and the application range and the operation conditions of the isotope marking are severely restricted. Therefore, it is highly desirable to provide a simple and effective CO2An isotope labeling device for expanding the application of isotope labeling technology in photosynthetic carbon assimilation and distribution.
SUMMERY OF THE UTILITY MODEL
The utility model discloses aim at solving above-mentioned technical problem to a certain extent at least, provide a simple and easy stable isotope gas marking device, the device convenient operation, the cost of manufacture is low.
The technical scheme of the utility model is that: a simple stable isotope gas marking device comprises a marking box and a cover arranged at the upper part of the marking box, wherein a cultivation mechanism, a beaker, a water pump and a triangular flask for loading lactic acid are arranged in the marking box;
the beaker, the water pump and the triangular flask are connected through pipelines, and the water pump is connected with an external power supply; the cultivation mechanism plants to be marked.
The utility model discloses in, cultivation mechanism is for cultivating box or basin, has in the beaker to be used for13C-labeled barium carbonate powder, lactic acid in the triangular flask is sucked into the beaker by a water pump, and the lactic acid and Ba in the beaker13CO3The powder reacts to give off the isotope-labelled13CO2A gas.
Furthermore, the marking box is made of organic glass. The marking box is made of organic glass with good light transmittance, so that an additional light source is not needed, and the marking box can be directly placed in natural conditions or places such as a greenhouse, a plant culture room and the like for testing. The cover of the marking box can be sealed by using an adhesive tape, so that the air tightness is ensured.
The device can calculate Ba added into the box according to the volume in the marking box13CO3The amount of the powder and the lactic acid is used, thereby accurately controlling the reaction in the marking box13CO2The concentration of the gas.
Furthermore, a fan is arranged on the side wall inside the marking box, and the fan is a storage battery or a dry battery fan. Preferably, the number of the fans is 4, and the fans are hung on the inner side wall of the marking box.
The utility model discloses in, in order to make the gas that the reaction produced can evenly distributed, four lateral walls tops are provided with four fans in the mark incasement. And the fan power supply is a storage battery or a dry battery, so that the limitation of an external power supply is avoided.
Furthermore, the beaker is arranged at the corner of the marking box, and the water pump is arranged at the position adjacent to the beaker.
The stable isotope gas marking device also comprises a water outlet pipe and a water suction pipe, wherein the beaker is connected with the water pump through the water outlet pipe, and the water pump is connected with the triangular flask filled with the lactic acid through the water suction pipe.
The utility model discloses in, the water pump is connected respectively and is absorbed water pipe and outlet pipe, and the lactic acid liquid level that absorbs water in the pipe other end immerges the triangular flask is below, and the outlet pipe other end stretches into the beaker of incasement.
Furthermore, a switch is arranged on the outer side wall of the marking box, a hole is formed in the side wall of the marking box, the switch is connected with an external power supply outside the marking box, and an electric wire connected with the switch is connected into the marking box through the hole and is connected with the water pump. One end of the switch is connected with an external power supply, and the other end of the switch penetrates through the hole in the box wall through an electric wire to be connected into the box so as to control the operation of the micro-sampling water pump.
The device accurately controls the adding amount of the lactic acid liquid in the triangular flask by controlling the running time of the water pump. In order to ensure the accurate liquid adding amount, the water pump is preferably provided with a direct current motor of 12V and a water pipe with the inner diameter of 1.5mm, so that the flow rate is ensured to be 0.5 ml/s.
Furthermore, a soft rubber plug is arranged on the hole. The soft rubber plug not only ensures the passing of the electric wire, but also ensures the air tightness of the whole device.
Furthermore, the cultivation mechanism is made of opaque organic glass or plastic. The surface layer of the cultivation mechanism is provided with a preservative film.
The utility model discloses in, cultivation mechanism material is all can for opaque organic glass box, plastics basin etc.. The cultivation mechanism can be filled with various growth media such as soil, sand, nutrient solution, matrix and the like, and the surface layer of the cultivation mechanism is sealed by a preservative film so as to prevent the isotope-labeled gas from polluting the growth media in the cultivation mechanism.
The utility model discloses a theory of operation: the plant to be marked is placed in the marking box, and the surface of the cultivation mechanism is provided with a preservative filmSealing to prevent contamination of the growth medium, and placing weighed Ba in the beaker13CO3Powder, the power supply of the fan is turned on, the cover of the marking box is covered, and the transparent adhesive tape is used for sealing; connecting an external power supply, starting a switch to operate a trace sample adding water pump, sucking lactic acid into the beaker in the marking box, and enabling the lactic acid and Ba in the small beaker13CO3The powder reacts to give off the isotope-labelled13CO2A gas; the reaction being produced by fans on four side walls of the chamber13CO2The gas is uniformly distributed in the box.
Compared with the prior art, the beneficial effects are: the utility model discloses a device need not extra light source, and the device is simple effective, and the volume size can design as required in a flexible way, the incasement13CO2The gas concentration can be adjusted according to the size of the box body to add Ba13CO3The dosage of the powder and the lactic acid is flexibly controlled, and the fan ensures13CO2The gas distribution is even, convenient operation, and the material is comparatively common, and the cost of manufacture is low.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
In the figure: 1-a lid; 2-marking the box; 3-a fan; 4-holes; 5-soft rubber plug; 6-external power supply; 7-beaker; 8-preservative film; 9-a cultivation mechanism; 10-a switch; 11-an electrical wire; 12-a water pump; 13-a suction pipe; 14-a water outlet pipe; 15-triangular flask.
Detailed Description
The drawings are for illustrative purposes only and are not to be construed as limiting the patent; for the purpose of better illustrating the embodiments, certain features of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted. The positional relationships depicted in the drawings are for illustrative purposes only and are not to be construed as limiting the present patent.
Example one
As shown in figure 1, the simple stable isotope gas marking device comprises a marking box 2 and a cover 1 arranged at the upper part of the marking box 2, wherein a cultivation mechanism 9, a beaker 7, a water pump 12 and a triangular flask 15 for loading lactic acid are arranged inside the marking box 2;
the beaker 7, the water pump 12 and the triangular flask 15 are connected through pipelines, and the water pump 12 is connected with an external power supply; the cultivation facilities 9 plant plants to be marked.
In this embodiment, the cultivating mechanism 9 is a cultivating box or a pot, and the beaker 7 is provided with a cover for covering the cultivating box or the pot13The lactic acid in the flask 15 was sucked into the beaker 7 by the water pump 12 to mix the lactic acid with Ba in the beaker 713CO3The powder reacts to give off the isotope-labelled13CO2A gas.
The marking box 2 is made of organic glass. The marking box is made of organic glass with good light transmittance, so that an additional light source is not needed, and the marking box can be directly placed in natural conditions or places such as a greenhouse, a plant culture room and the like for testing. The cover of the marking box can be sealed by using an adhesive tape to ensure air tightness.
Moreover, the device can calculate Ba added in the box according to the volume in the marking box13CO3The amount of the powder and the lactic acid is used, thereby accurately controlling the reaction in the marking box13CO2The concentration of the gas.
As shown in fig. 1, four fans 3 are disposed above four side walls in the marking box 2 in order to uniformly distribute the gas generated by the reaction. And the power supply of the fan 3 is a storage battery or a dry battery, so that the limitation of an external power supply is avoided.
In this embodiment, the water pump 12 is connected to a water suction pipe 13 and a water outlet pipe 14, respectively, the other end of the water suction pipe 13 is immersed below the lactic acid liquid level in the triangular flask 15, and the other end of the water outlet pipe 14 extends into the beaker 7 in the tank.
One end of a switch 10 is connected with an external power supply 6, and the other end of the switch passes through the hole 4 of the box wall through an electric wire 11 to be connected into the box, so as to control the operation of the micro sample-adding water pump 12.
The device accurately controls the adding amount of the lactic acid liquid in the triangular flask 15 by controlling the running time of the water pump 12. In order to ensure the accurate liquid adding amount, it is preferable that the water pump 12 is configured with a DC motor of 12V and a water pipe with an inner diameter of 1.5mm, thereby ensuring a flow rate of 0.5 ml/s.
The hole 4 is also provided with a soft rubber plug 5. The soft rubber plug 5 not only ensures the passing of the electric wire 11, but also ensures the air tightness of the whole device.
In this embodiment, the cultivation mechanism 9 is made of an opaque organic glass box, a plastic basin, or the like. The cultivation mechanism 9 can be filled with various growth media such as soil, sand, nutrient solution, matrix and the like, and the surface layer of the cultivation mechanism 9 is sealed by a preservative film 8 to prevent the isotope-labeled gas from polluting the growth media in the cultivation mechanism.
Specifically, the specific structure and principle of the present device may be as follows.
The length, width and height of the marking box 2 are respectively 50cm multiplied by 52cm multiplied by 50cm, so that the total volume in the box is 130L; the length and the width of the marking box cover 1 are respectively 50cm multiplied by 52 cm; the plant material is semen glycines and semen Maydis; the volume of the beaker 7 is 50mL, and 0.5gBa is filled in the beaker 713CO3Powder (2.54mmol) placed in a labeling box; the other flask 15 contains 50mL of lactic acid liquid (lactic acid content 85% -90%). Switch 10 pastes on the case lateral wall with the help of powerful glue, external power supply 6 is connected to one end, other end connecting wire 11, switch 10 below 5cm department has the diameter to be 1.2cm hole 4, electric wire 11 is connected to the water pump 12 of incasement through hole 4, water pump 12 rated voltage is 12V, be furnished with and absorb water pipe 13 and outlet pipe 14, length is 25cm, the internal diameter is 1.5mm, the external diameter is 3.5mm, it stretches into the triangular flask bottom that is equipped with lactic acid liquid to absorb water pipe one end, the other end links to each other with the water pump, outlet pipe one end links to each other with the water pump, the other end is connected to and is equipped13CO3In the powder beaker, the liquid flow rate of the device is 0.5mL/s, so that 5mL of lactic acid (the content of lactic acid is 85% -90%, the total amount of lactic acid is 56.6mmol) can be output from the water outlet pipe after 10s, and the lactic acid is sufficient, so that the chemical reaction equation Ba is adopted13CO3+2CH3CH(OH)COOH＝(CH3CH(OH)COO)2Ba+13CO2+H2O available, produced in the beaker 713CO2Is 2.54 × 24.5-62.23 mL-0.06223L (25 ℃, molar volume of gas at standard atmospheric pressure is about 24.5L/mol), so the total volume produced in the tank is13CO2Gas concentrationDegree of 0.06223 ÷ 130 ═ 4.79 × 10-4L/L is 479 mu L/L; the length, width and height of the cultivation mechanism 9 are respectively 13cm multiplied by 12 cm; the marking time period is nine am to three pm, and is six hours in total; the four fans 3 are adhered to the inner side wall of the marking box by strong glue, and the working time is more than six hours; and after the marking is finished, taking out the cultivation box/pot, opening the preservative film, and collecting samples after three days of ventilation.
The operation of the apparatus of the present embodiment may be such that the marker box 2 is first prepared; then sealing the surface layer of the cultivation mechanism 9 by using a preservative film 8, and winding the preservative film on the stem part of the seedling for more than one circle for sealing so as to prevent the isotope gas from polluting the growth medium; placing the cultivation mechanism 9 into the marking box 2, adding Ba into the beaker 713CO3The water pump 12, the electric wire 11, the water suction pipe 13 and the water outlet pipe 14 are installed and connected to ensure that the device is watertight. Turning on a power supply of the fan 3, covering the cover 1 of the marking box, and sealing; the external power supply 6 is switched on, the switch 10 is switched on, and the water pump 12 is operated to generate13CO2Gas is used, the switch is closed after timing for 10s, and the external power supply 6 is disconnected; the labeling box 2 is illuminated and cultured in the culture room, and is labeled for 6 hours.
Example two
In this embodiment, the connection mode of the marking box 2 and the cover 1 is hinged, the cover 1 can be conveniently rotated on the upper part of the marking box 2 to close the marking box 2, the connection mode is simple and quick, and only a hinge mechanism needs to be additionally installed.
The rest of the structure and operation principle of the present embodiment are the same as those of the first embodiment, and will not be described in detail herein.
It is obvious that the above embodiments of the present invention are only examples for clearly illustrating the present invention, and are not limitations to the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.
Claims (10)
1. A simple stable isotope gas marking device is characterized by comprising a marking box (2) and a cover (1) arranged at the upper part of the marking box (2), wherein a cultivation mechanism (9), a beaker (7), a water pump (12) and a triangular flask (15) for loading lactic acid are arranged inside the marking box (2);
the beaker (7), the water pump (12) and the triangular flask (15) are connected through pipelines, and the water pump (12) is connected with an external power supply; the cultivation mechanism (9) plants the plants to be marked.
2. A simple stable isotope gas labeling apparatus as recited in claim 1, further comprising: the marking box (2) is made of organic glass.
3. A simple stable isotope gas labeling apparatus as recited in claim 2, further comprising: the fan (3) is arranged on the side wall inside the mark box (2), and the fan (3) is a storage battery or a dry battery fan.
4. A simple stable isotope gas labeling apparatus as recited in claim 3, further comprising: the beaker (7) is arranged at the corner of the marking box (2), and the water pump (12) is arranged at the position adjacent to the beaker (7).
5. A simple stable isotope gas labeling apparatus as recited in claim 4, further comprising: the stable isotope gas labeling device also comprises a water outlet pipe (14) and a water suction pipe (13), the beaker (7) and the water pump (12) are connected by the water outlet pipe (14), and the water pump (12) is connected with the triangular flask (15) filled with lactic acid by the water suction pipe (13).
6. A simple stable isotope gas labeling apparatus as recited in claim 1, further comprising: the lateral wall of mark case (2) be equipped with switch (10), the lateral wall of mark case (2) on open and to have hole (4), switch (10) are connected external power source (6) outside mark case (2), electric wire (11) of being connected with switch (10) are passed through hole (4) and are inserted in mark case (2), are connected with water pump (12).
7. A simple stable isotope gas labeling apparatus as recited in claim 6, further comprising: the hole (4) is also provided with a soft rubber plug (5).
8. A simple stable isotope gas labeling apparatus according to any one of claims 1 to 7, further comprising: the cultivation mechanism (9) is made of opaque organic glass or plastic.
9. A simple stable isotope gas labeling apparatus according to any one of claims 1 to 7, further comprising: the surface layer of the cultivation mechanism (9) is provided with a preservative film (8).
10. A simple stable isotope gas labeling apparatus as recited in claim 3, further comprising: the number of the fans (3) is 4, and the fans are suspended on the inner side wall of the marking box (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921927737.2U CN211741195U (en) | 2019-11-08 | 2019-11-08 | Simple stable isotope gas marking device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921927737.2U CN211741195U (en) | 2019-11-08 | 2019-11-08 | Simple stable isotope gas marking device |
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| Publication Number | Publication Date |
|---|---|
| CN211741195U true CN211741195U (en) | 2020-10-23 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201921927737.2U Expired - Fee Related CN211741195U (en) | 2019-11-08 | 2019-11-08 | Simple stable isotope gas marking device |
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|---|---|
| CN (1) | CN211741195U (en) |
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2019
- 2019-11-08 CN CN201921927737.2U patent/CN211741195U/en not_active Expired - Fee Related
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Granted publication date: 20201023 Termination date: 20211108 |