CN211741155U - Electrophoresis separation and ultraviolet absorption detection system based on embedded chip structure - Google Patents

Abstract

An electrophoresis separation and ultraviolet absorption detection system based on an embedded chip structure comprises: the embedded electrophoresis chip and the collimating lens which is respectively arranged at the upper side and the lower side of the embedded electrophoresis chip and respectively connected with the optical detector and the ultraviolet light source, wherein: the embedded electrophoresis chip is provided with a separation capillary tube with a hollow square column structure, and the optical detector and the ultraviolet light source are respectively positioned at two sides of the capillary tube. The utility model discloses the analysis sample that can be quick adopts ultraviolet absorption to detect simultaneously, avoids loaded down with trivial details derivatization process.

Description

Electrophoresis separation and ultraviolet absorption detection system based on embedded chip structure

Technical Field

The utility model relates to a technique in sample detection field specifically is an electrophoresis separation and ultraviolet absorption detecting system based on embedded chip structure.

Background

In the electrophoretic analysis process of samples in pharmacy, chemical industry, clinic and the like, the method can quickly and efficiently analyze the samples, avoid the complicated process of derivatizing the fluorescent groups on the samples to be analyzed, realize high-sensitivity detection, and is expected by analysis and detection technicians. The conventional capillary electrophoresis and chip electrophoresis cannot satisfy the above expectations at the same time. Since the separation capillary required for a typical capillary electrophoresis apparatus is about 30cm to 80cm long and the separation time is about half an hour to 1 hour. The general detection adopts ultraviolet absorption detection, the inner diameter of the capillary is about 50 mu m to 100 mu m, the inner diameter is the optical path of the ultraviolet absorption, the optical path has moderate length, and reasonable sensitivity can be provided. For general chip electrophoresis, channels in a chip are generally processed by photolithography and chemical etching methods, and the aspect ratio (the ratio of the channel width to the channel depth) is often small, so if a sample is detected by using an ultraviolet absorption method, the ultraviolet absorption sensitivity is low because the optical path (the length of the optical path is equal to the channel depth) is short, and general chip electrophoresis often uses fluorescence detection. The fluorescence detection has higher sensitivity, but has the defects, for example, most of samples to be detected do not have autofluorescence, and the samples are required to be subjected to derivatization treatment, so that the samples to be detected are connected with groups capable of generating fluorescence. The derivatization process is complicated, and additional impurities can be generated to interfere the detection result.

SUMMERY OF THE UTILITY MODEL

The utility model discloses it is not enough to the above-mentioned that prior art exists, an electrophoresis separation and ultraviolet absorption detecting system based on embedded chip structure is proposed, adopt unique embedded chip structure, cooperate the cross passageway electricity sampling structure and a segment separation capillary of chip, make and to inject the sample into the separation capillary with very narrow sample stopper, realize quick separation, the loaded down with trivial details fluorescence detection method has been avoided on detection method simultaneously, adopt the ultraviolet absorption to detect, cross section in the capillary is the ultraviolet absorption optical path, realize the sensitivity similar with ordinary capillary.

The utility model discloses a realize through following technical scheme:

the utility model discloses a: the embedded electrophoresis chip and the collimating lens which is respectively arranged at the upper side and the lower side of the embedded electrophoresis chip and respectively connected with the optical detector and the ultraviolet light source, wherein: the embedded electrophoresis chip is provided with a separation capillary tube with a hollow square column structure, and the optical detector and the ultraviolet light source are respectively positioned at two sides of the capillary tube.

The embedded electrophoresis chip comprises: an upper cover plate, a lower substrate and a separation capillary disposed therebetween, wherein: the upper cover plate and the lower base plate are respectively provided with a channel group and a through hole group.

The through hole group arranged on the upper cover plate comprises: main through-hole and supplementary inlet hole correspond the passageway group that sets up on the upper cover plate and include: a main channel located on both sides of the main through hole.

The through hole group arranged on the lower substrate comprises: the main through-hole that is linked together with the fixed slot corresponds the passageway group that sets up on the infrabasal plate and includes: and the auxiliary channel is in a tree structure and is communicated with the fixing groove.

Drawings

FIG. 1 is a schematic view of the overall structure of the apparatus;

FIG. 2 is an exploded schematic view of a chimeric electrophoresis chip;

FIG. 3 is a schematic view of an upper cover plate;

FIG. 4 is a schematic view of a lower substrate;

FIG. 5 is a schematic view of a lower substrate;

FIG. 6 is a schematic view of a separation capillary installation;

FIG. 7 is a schematic diagram of an exemplary test;

FIG. 8 is a diagram of the optical path of the embodiment;

in the figure: the device comprises a collimating lens 1, a mosaic electrophoresis chip 2, an optical fiber 3, a PMT detector 4, an ultraviolet light source 5, a sample absorption capillary 6, a channel group 7, a through hole group 8, an ultraviolet absorption light path 9, an adapter port 10, an upper cover plate 201, a separation capillary 202, a lower substrate 203, a fixing groove 2031, a fixing port 2032 and a capillary outer wall 2021.

Detailed Description

As shown in fig. 1, the present embodiment relates to an electrophoresis separation and ultraviolet absorption detection system based on an embedded chip structure, which includes: the embedded electrophoresis chip 2 and the collimating lens 1 respectively arranged at the upper and lower sides thereof and respectively connected with the PMT detector 4 and the ultraviolet light source 5, wherein: the embedded electrophoresis chip 2 is provided with a separation capillary 202 (the separation capillary is 5cm long, the length of the inner edge of the hollow square column is 100um, and the length of the outer edge is 360um) with a hollow square column structure, two collimating lenses are respectively positioned at the upper side and the lower side of the capillary 202, one lens focuses deuterium lamp ultraviolet light led out by an optical fiber on the capillary, the other lens is used for collecting ultraviolet light penetrating through the capillary and transmitting the ultraviolet light to a PMT detector through the optical fiber, when a sample absorbing the ultraviolet light passes through the capillary, the change of the concentration of the sample in the capillary is converted into the change of light intensity transmitted to the PMT, and the PMT is converted into an electric signal with voltage change.

As shown in fig. 2, the chimeric electrophoresis chip 2 includes: an upper cover plate 201 (made of polymer such as PDMS), a lower base plate 203 (made of polymer such as PDMS), and a separation capillary 202 (made of fused silica) disposed therebetween, wherein: the upper cover plate 201 and the lower substrate 203 are respectively provided with a channel group 7 and a through hole group 8.

As shown in fig. 3, the through hole group 8 provided in the upper cover plate 201 includes: main through-hole 801 (diameter 10mm) and supplementary inlet hole 802 (diameter 5mm), hole 803 (diameter 4mm), hole 804 (diameter 4mm), hole 805 (diameter 4mm), the passageway group 7 that corresponds to set up on upper cover plate 201 includes: a main channel 701 on both sides of the main via 801.

As shown in fig. 4, the through hole group 7 provided on the lower substrate 203 includes: the main via 801 communicating with the fixing groove 2031, and the channel group 7 correspondingly disposed on the lower substrate 203, includes: an auxiliary channel 702 (channel width 100um, depth 100um) of a tree structure communicating with the fixing groove 2031. The lower substrate 203 is provided with a fixing groove 2031, two ends of which are respectively communicated with the channel group 7 and the through hole group 8 and are used for placing the separation capillary 202.

As shown in fig. 5, the auxiliary channel 702 includes a channel branch 7021 (width 100um, depth 100um) and a channel trunk 7022 (width 370um, depth 230um) connected to the fixing groove 2031, wherein: the channel trunk 7022 has a smaller cross-sectional area than the fixing groove 2031, thereby having greater compatibility with dimensional errors of the separation capillary placed therein.

As shown in fig. 6, a fixing hole 2032 is formed on the lower substrate 203, and the fixing hole 2032 is specifically located on the other side of the main through hole 801 corresponding to the fixing groove 2031.

As shown in FIG. 7, the lower substrate 203 is provided with an adapter 10 for connecting the sample capillary 6.

The device carries out detection in the following modes: a small section of capillary is embedded into a pre-processed pore channel in the chip, and the upper substrate and the lower substrate are aligned and fixed to form the capillary embedded electrophoresis chip. Additional channels are formed in the chip perpendicular to and in cross contact with the capillaries to form a cross structure. The crossed cross structure is used as an electric injection port, and the small section of capillary is used as an electrophoresis separation channel of a detected sample. The sample is sucked into the channel 7021 through the sample sucking capillary 6 by applying negative pressure to the auxiliary sample inlet hole 802, then 100V voltage is applied to the channel which is crossed with the sample inlet capillary (vertical to the separation capillary), so that the sample in the channel moves in the channel which is crossed with the separation capillary (vertical to the separation capillary), the sample is prevented from diffusing to a separation channel, then 300V voltage is immediately applied between the outlet end of the separation capillary and the hole 804, and the 100V voltage in the front is closed, so that the sample injection solution is injected into the capillary in a flat plunger shape (the length of the plunger is basically consistent with the width of the channel 7021 and is about 100um) and then is subjected to electrophoretic separation.

The collimator lens 1 connected to the light source as shown in fig. 8 irradiates upward from the bottom of the separation capillary 202, and the PMT detector 4 located above the separation capillary 202 collects corresponding optical path information. Because the sample plunger in the capillary is short, a very short capillary is required for high resolution and very fast analysis times. Meanwhile, because the capillary is a square hollow capillary and the inner edge is 100um, the detection optical path at the position is 100um, the larger aspect ratio has a longer optical path than a smaller aspect ratio channel of the common chip electrophoresis, and the fused quartz material of the capillary has good transmittance to ultraviolet light and can be used as a detector in an ultraviolet absorption mode. Compared with the common capillary electrophoresis, the system in the utility model can greatly reduce the sample analysis time, and can realize the ultraviolet absorption sensitivity similar to the capillary.

This device adopts the cross structure electricity of chip to advance a kind interface and separation capillary complex mode for the sample stopper width of electricity appearance reduces greatly, is about 100 um. In the conventional capillary sample injection mode, the length of the sample plug is close to 1 mm. The length of the sample plug is inversely proportional to the number of theoretical plates for capillary separation, so that the conventional capillary needs a separation degree of 50cm long, and the separation length of about 5cm is only needed in the device, so that the separation time is greatly shortened and is only about one tenth of that of the conventional capillary. Meanwhile, because the optical detection part is still on the capillary tube instead of the chip, the ultraviolet absorption detection structure of the common capillary tube can be adopted, and the optical path of 100um can provide the same sensitivity as that of the common capillary tube

The foregoing embodiments may be modified in various ways by those skilled in the art without departing from the spirit and scope of the present invention, which is not limited by the above embodiments but is to be accorded the full scope defined by the appended claims, and all such modifications and variations are within the scope of the invention.

Claims (8)

1. An electrophoresis separation and ultraviolet absorption detection system based on embedded chip structure, characterized by comprising: the embedded electrophoresis chip and the collimating lens which is respectively arranged at the upper side and the lower side of the embedded electrophoresis chip and respectively connected with the optical detector and the ultraviolet light source, wherein: the embedded electrophoresis chip is provided with a separation capillary tube with a hollow square column structure, and the optical detector and the ultraviolet light source are respectively positioned at two sides of the capillary tube.

2. The embedded chip structure-based electrophoretic separation and ultraviolet absorption detection system as claimed in claim 1, wherein the embedded electrophoretic chip comprises: an upper cover plate, a lower substrate and a separation capillary disposed therebetween, wherein: the upper cover plate and the lower base plate are respectively provided with a channel group and a through hole group.

3. The system of claim 2, wherein the set of through holes disposed on the top cover plate comprises: main through-hole and supplementary inlet hole correspond the passageway group that sets up on the upper cover plate and include: a main channel located on both sides of the main through hole.

4. The system of claim 2, wherein the through hole set disposed on the lower substrate comprises: the main through-hole that is linked together with the fixed slot corresponds the passageway group that sets up on the infrabasal plate and includes: and the auxiliary channel is in a tree structure and is communicated with the fixing groove.

5. The electrophoresis separation and ultraviolet absorption detection system based on embedded chip structure as claimed in claim 2, wherein the lower substrate is provided with a fixing groove for installing a separation capillary, both ends of which are respectively communicated with the channel group and the through hole group.

6. The system according to claim 4, wherein the auxiliary channel comprises a channel branch and a channel trunk connected to the fixing groove, and wherein: the sectional area of the channel trunk is smaller than that of the fixed groove.

7. The system according to claim 4, wherein the lower substrate has a fixing hole, and the fixing hole is located on the other side of the main through hole corresponding to the fixing groove.

8. The system according to claim 2, wherein the lower substrate is provided with an adapter for connecting a sample capillary.

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