CN211725636U

CN211725636U - Culture medium stirring and filtering device

Info

Publication number: CN211725636U
Application number: CN201922394147.4U
Authority: CN
Inventors: 陈廷涛
Original assignee: Nanchang University
Current assignee: Nanchang University
Priority date: 2019-12-27
Filing date: 2019-12-27
Publication date: 2020-10-23
Anticipated expiration: 2029-12-27

Abstract

The utility model discloses a culture medium stirring and filtering device, which comprises a stirring device and a filtering device, the filtering device is positioned at the lower end of the stirring device, a stirring paddle is arranged in the stirring device, the stirring paddle is driven by a motor, ozone generators are arranged below the two sides of the stirring device, the ozone generator is connected with the blowing nozzle through a small air pump, the blowing nozzle is positioned at the bottom in the stirring device, the middle side of the bottom of the stirring device is provided with a communicating pipe, the communicating pipe is provided with an electromagnetic valve, a filter screen is arranged in the filtering device, the filter screen is located communicating pipe under, and the filter screen middle part is high, and is low all around, the utility model discloses structural design is simple, reasonable, and is high-efficient convenient, easily operates, will stir and filter two processes and combine together, has improved culture medium preparation efficiency on the one hand, and on the other hand has reduced the contaminated risk of culture medium.

Description

Culture medium stirring and filtering device

Technical Field

The utility model belongs to the technical field of the cell culture technique and specifically relates to a culture medium stirring filter equipment.

Background

The culture medium is a nutrient substance artificially prepared according to the nutrient requirement of the microorganism according to a proportion. The culture medium generally contains water, a nitrogen source, inorganic salts, a carbon source, growth factors, agar and the like, some nutrient components are not easy to dissolve, continuous stirring is needed in the preparation process to accelerate dissolution, then impurities are filtered and removed, stirring and filtering operation are carried out in two working procedures by a conventional method, stirring is carried out in one working procedure, and then manual transportation is carried out in the other working procedure, so that the method is low in efficiency, and the risk of culture medium pollution is increased.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a culture medium stirring filter equipment to solve the problem that proposes among the above-mentioned background art. In order to achieve the above object, the utility model provides a following technical scheme:

a culture medium stirring and filtering device comprises a stirring device and a filtering device, wherein the filtering device is positioned at the lower end of the stirring device, the top of the stirring device is fixedly connected with a motor, the output end of the motor is fixedly connected with a rotating shaft, the rotating shaft is directly communicated with the inner bottom end of the stirring device, a stirring paddle is arranged on the rotating shaft, the stirring paddle is of a frame type, an exhaust pipe is arranged on the right side of the motor and extends into the stirring device, an exhaust pump is arranged on the exhaust pipe, a liquid injection pipe is arranged on the left side of the motor and extends into the stirring device, a liquid injection valve is arranged on the liquid injection pipe, a feeding pipe is arranged on the side wall on the left side of the stirring device, the feeding pipe is of a circular arc structure, a feeding port cover is movably connected onto the feeding pipe, an ozone generator is arranged below two sides of the stirring, the small air pump is located at the lower side of the stirring device, the air blowing nozzle is located at the bottom in the stirring device, a waterproof breathable film is arranged on the air blowing nozzle, the middle of the bottom of the stirring device is provided with a communicating pipe, the bottom of the communicating pipe stretches into the inside of the filtering device, the communicating pipe is provided with an electromagnetic valve, a filter screen is installed in the filtering device and located under the communicating pipe, the middle of the filter screen is high, the periphery of the filter screen is low, the right side of the bottom of the filtering device is provided with a liquid outlet pipe, and.

In this embodiment: install pressure sensor and acidimeter in the agitating unit, the agitating unit left side is provided with control panel, be provided with the controller in the control panel, the last display screen that is provided with of control panel, display screen below are provided with solenoid valve aperture regulating switch, the controller is connected with solenoid valve, pressure sensor, acidimeter and solenoid valve aperture regulating switch electricity respectively.

In this embodiment: the controller is of model C8051F 330.

In this embodiment: the filter screen is made of double-layer gauze.

Compared with the prior art, the beneficial effects of the utility model are that: (1) the ozone generator generates ozone, and the ozone enters the stirring device through the blowing nozzle under the pressurization effect of the small air pump, so that the effects of sterilization and disinfection are achieved, the raw material flow is promoted, and the stirring and mixing efficiency is improved; (2) install the filter screen in the filter equipment, high in the middle of the filter screen, low all around, the impurity that the filter screen was strained can erode all around, can guarantee not blockking up in the middle of the filter screen, remains the filter capacity all the time. (3) An acidity meter is arranged in the stirring device and used for detecting the pH value in the stirring device, and an acid-base solution is added through a liquid injection pipe to adjust the pH value of the culture medium, so that the pH value of the culture medium can meet the culture requirement.

Drawings

FIG. 1 is a schematic structural view of the present invention;

reference numerals: the device comprises a motor 1, a liquid injection pipe 2, a liquid injection valve 3, a feeding pipe 4, an air extraction pipe 5, an air extraction pump 6, a control panel 7, a display screen 8, a solenoid valve opening adjusting switch 9, an ozone generator 10, a small air pump 11, an air blowing nozzle 12, a waterproof air-permeable film 13, a solenoid valve 14, a communicating pipe 15, a filter screen 16, a manual valve 17, a liquid outlet pipe 18, a rotating shaft 19, a stirring paddle 20, a pressure sensor 21, an acidimeter 22, a stirring device 23, a filtering device 24 and a controller 25.

Detailed Description

The utility model discloses the following will be explained in detail with the accompanying drawings, as shown in fig. 1, a culture medium stirring filter equipment, including agitating unit 23 and filter equipment 24, filter equipment 24 is located agitating unit 23's lower extreme, agitating unit 23 top fixed connection motor 1, motor 1's output fixed connection axis of rotation 19, axis of rotation 19 leads to bottom in the agitating unit 23, sets up stirring rake 20 in the axis of rotation 19, stirring rake 20 is the frame, and frame stirring rake 20 effective stirring space is big, and the stirring of nearly full volume is convenient for raw materials misce bene.

In this embodiment: 1 right side of motor sets up exhaust tube 5, inside 5 one ends of exhaust tube stretched into agitating unit 23, be provided with aspiration pump 6 on 5 other ends of exhaust tube, when 23 internal pressure of agitating unit were high on the left, aspiration pump 6 can extract a part atmospheric pressure, the reducing mechanism internal pressure, and 1 left side of motor is provided with annotates liquid pipe 2, annotate that 2 one end of liquid pipe stretched into agitating unit 23 inside, annotate and be provided with on 2 other ends of liquid pipe and annotate liquid valve 3, annotate liquid pipe 2 top and be the bell mouth, be convenient for add acid-base solution, be difficult to spill outside, be provided with inlet pipe 4 on the lateral wall on 23 left sides of agitating unit, inlet pipe 4 is the circular arc structure, swing joint has the inlet flap.

In this embodiment: the ozone generator 10 is arranged below two sides of the stirring device 23, the ozone generator 10 is connected with one end of the small air pump 11, the other end of the small air pump 11 is connected with the air blowing nozzle 12, the small air pump 11 is arranged at the lower side of the stirring device 23, the air blowing nozzle 12 is arranged at the bottom in the stirring device 23, the air blowing nozzle 12 is provided with a waterproof breathable film 13, the ozone generator 10 generates ozone, the ozone enters the stirring device 23 through the air blowing nozzle 12 under the pressurization effect of the small air pump 11, on one hand, the sterilization and disinfection effects are achieved, on the other hand, the raw material flow is promoted, the stirring and mixing efficiency is improved, the bottom inside of the stirring device 23 is provided with a communicating pipe 15, the bottom end of the communicating pipe 15 extends into the inside of the filtering device 24, the communicating pipe 15 is provided with an electromagnetic valve 14, a filtering screen, the filter screen 16 middle part is high, and is low all around, and the impurity that filter screen 16 was strained can erode all around, can guarantee not blockked up in the middle of the filter screen 16, remains the filter capacity all the time, and 24 bottom right sides of filter equipment set up drain pipe 18, set up manual valve 17 on the drain pipe 18.

In this embodiment: install pressure sensor 21 and acidimeter 22 in agitating unit 23, agitating unit 23 left side is provided with control panel 7, be provided with controller 25 in the control panel 7, the last display screen 8 that is provided with of control panel 7, display screen 8 below are provided with two solenoid valve aperture regulating switch 9, and left realization valve aperture increases, and the realization valve aperture on right side reduces, controller 25 is connected with solenoid valve 14, pressure sensor 21, acidimeter 22 and solenoid valve aperture regulating switch 9 electricity respectively, and the signal transmission that pressure sensor 21 and acidimeter 22 detected gives controller 25, and controller 25 sends the signal again for display screen 8, and display screen 8 shows agitating unit 23 internal pressure, pH value and the aperture of solenoid valve 14.

In this embodiment: the controller 25 is of the type C8051F 330.

In this embodiment: the material of the filter screen 16 is double-layer gauze.

The utility model discloses the theory of operation is: raw materials enter the interior of the stirring device 23 from the feeding pipe 4, after the power is supplied to the motor 1, the stirring paddle 20 is driven to rotate, the raw materials are uniformly mixed, the ozone generator 10 generates ozone, under the pressurization effect of the small air pump 11, the raw materials enter the interior of the stirring device 23 through the blowing nozzle 12, on one hand, the sterilization and disinfection effects are achieved, on the other hand, the raw materials are promoted to flow, the stirring and mixing efficiency is improved, after the raw materials are uniformly mixed, the opening degree of the electromagnetic valve 14 is controlled through the electromagnetic valve opening degree adjusting switch 9, the flow rate of the culture media entering the filtering device 24 is controlled, the middle of the filter screen 16 is high, the periphery of the filter screen 16 is low, impurities filtered by the filter screen 16 can be flushed around, the middle of the filter screen 16 can be guaranteed not to be.

The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included within the protection scope of the present invention.

Claims

1. A culture medium stirring and filtering device is characterized in that: including agitating unit (23) and filter equipment (24), filter equipment (24) are located the lower extreme of agitating unit (23), agitating unit (23) top fixed connection motor (1), the output fixed connection axis of rotation (19) of motor (1), bottom in axis of rotation (19) the straight-through agitating unit (23), set up stirring rake (20) on axis of rotation (19), stirring rake (20) are the frame, and motor (1) right side sets up exhaust tube (5), inside exhaust tube (5) one end stretched into agitating unit (23), was provided with aspiration pump (6) on exhaust tube (5) the other end, and motor (1) left side is provided with annotates liquid pipe (2), annotate inside liquid pipe (2) stretched into agitating unit (23), be provided with on annotating liquid pipe (2) inlet pipe (3), be provided with inlet pipe (4) on agitating unit (23) left side lateral wall, the inlet pipe (4) is the circular arc structure, swing joint has the feed inlet flap on inlet pipe (4), and the both sides below of agitating unit (23) is provided with ozone generator (10), ozone generator (10) are connected with the one end of little air pump (11), and the other end of little air pump (11) is connected blow shower nozzle (12), little air pump (11) are located the downside of agitating unit (23), blow shower nozzle (12) are located agitating unit (23) bottom, are provided with waterproof ventilated membrane (13) on blow shower nozzle (12), and agitating unit (23) bottom inboard is provided with communicating pipe (15), and the inside of filter equipment (24) is stretched into to communicating pipe (15) bottom, is provided with solenoid valve (14) on communicating pipe (15), installs filter screen (16) in filter equipment (24), filter screen (16) are located communicating pipe (15) under, the middle part of the filter screen (16) is high, the periphery is low, a liquid outlet pipe (18) is arranged on the right side of the bottom of the filter device (24), and a manual valve (17) is arranged on the liquid outlet pipe (18).

2. The culture medium stirring and filtering device as set forth in claim 1, wherein: install pressure sensor (21) and acidimeter (22) in agitating unit (23), agitating unit (23) left side is provided with control panel (7), be provided with controller (25) in control panel (7), be provided with display screen (8) on control panel (7), display screen (8) below is provided with solenoid valve aperture regulating switch (9), controller (25) are connected with solenoid valve (14), pressure sensor (21), acidimeter (22) and solenoid valve aperture regulating switch (9) electricity respectively.

3. The culture medium stirring and filtering device as claimed in claim 2, wherein: the controller (25) is of the type C8051F 330.

4. The culture medium stirring and filtering device as set forth in claim 1, wherein: the material of the filter screen (16) is double-layer gauze.