CN211713126U

CN211713126U - Cell hypoxia culture device

Info

Publication number: CN211713126U
Application number: CN202020070263.0U
Authority: CN
Inventors: 郇延军; 屈佳丹; 王祥雨; 孙铭菊
Original assignee: Qingdao Agricultural University
Current assignee: Qingdao Agricultural University
Priority date: 2020-01-13
Filing date: 2020-01-13
Publication date: 2020-10-20
Anticipated expiration: 2030-01-13
Also published as: AU2020103399A4

Abstract

The utility model discloses a cell hypoxia culture apparatus, it includes box, controller and oxygen concentration meter, the upper end of box is provided with the case lid, is provided with a plurality of cultivation boxes from top to bottom in the box, is provided with the pivot on every cultivation box's the axis, is provided with the puddler in the pivot, and the puddler tip extends in to cultivating the box. The upper end of the rotating shaft is provided with a driving tooth, and the bottom end of the rotating shaft is provided with a tooth groove matched with the driving tooth. The rotating shaft at the lowest end is connected with a driving motor. The side wall and the bottom wall of the box body are provided with heat preservation chambers, the upper ends of the heat preservation chambers are connected with a water inlet, the bottom ends of the heat preservation chambers are connected with a water outlet, and valves are arranged on the water inlet and the water outlet. Two limiting rods are arranged on the bottom wall of the box body, and corresponding limiting holes are formed in the side wall of each culture box. The utility model has the characteristics of convenient, the heat preservation of taking and can cultivate in a large number.

Description

Cell hypoxia culture device

Technical Field

The utility model relates to a cell hypoxia culture device.

Background

The concentration of required oxygen is different when the cell of different positions of animal is cultivateed, and required normal oxygen content generally can be less than the oxygen content in the air, and the low oxygen is cultivateed and is had an important effect to medical research, and some cells need put into a bar magnet that has the polytetrafluoroethylene cladding in the culture dish in the process of cultivating to in sending the culture dish into the thermostated container, stir the cultivation on the magnetic stirrers, can meet following problem in the culture process: 1. the culture dish can only be singly cultured when in culture, and each magnetic stirrer can only stir one culture dish once, and is inefficient. 2. The magnetic bar is attached to the bottom wall of the culture dish, and the cells are easily crushed during the stirring process. Therefore, the designed cell hypoxia culture device which is convenient to take, can preserve heat and can be used for mass culture has wide market prospect.

SUMMERY OF THE UTILITY MODEL

The utility model provides a cell hypoxia culture device which is convenient to take, can keep warm and can be cultured in a large number.

The utility model provides a technical scheme that its technical problem adopted is:

the cell hypoxia culture device comprises a box body, a controller and an oxygen concentration meter, wherein a box cover is arranged at the upper end of the box body, a plurality of culture boxes are arranged up and down in the box body, a rotating shaft is arranged on the axis of each culture box, a stirring rod is arranged on each rotating shaft, and the end part of each stirring rod extends into each culture box; the upper end of the rotating shaft is provided with driving teeth, and the bottom end of the rotating shaft is provided with tooth grooves matched with the driving teeth; the rotating shaft at the lowest end is connected with a driving motor; the side wall and the bottom wall of the box body are provided with heat preservation chambers, the upper end of each heat preservation chamber is connected with a water inlet, the bottom end of each heat preservation chamber is connected with a water outlet, and valves are arranged on the water inlets and the water outlets;

two limiting rods are arranged on the bottom wall of the box body, and a limiting hole is correspondingly arranged on the side wall of each culture box; the cover body is provided with an air inlet and an air outlet.

Furthermore, an annular groove is formed in the culture box; the rotating shaft is sleeved with a bearing, the inner ring of the bearing is fixedly connected with the rotating shaft, and the outer ring of the bearing is fixedly connected with the culture box.

Further, the bottom of the stirring rod is provided with a spherical head.

Furthermore, a driving motor control button and a display screen are arranged on the controller.

The utility model has the advantages that:

through vertically setting up a plurality of culture boxes in the box, can cultivate a large amount of cells simultaneously. Through setting up the drive tooth in the upper end of pivot, the bottom sets up the tooth's socket, during the use, connects a plurality of cultivation boxes in proper order through drive tooth and tooth's socket, still can realize the stirring to every cultivation box when realizing cultivateing in a large number, during the use, only need bottom half installation a driving motor drive can. Through being provided with the heat preservation cavity on the lateral wall and the diapire at the box, keep warm to the indoor water injection of heat preservation cavity when using. The oxygen content in the box body can be observed at any time by arranging the display screen on the controller.

Drawings

FIG. 1 is a schematic perspective view of the present invention;

FIG. 2 is a schematic perspective view of the present invention;

in the figure, a box body 1, a heat preservation chamber 11, a water inlet 111, a water outlet 112, a valve 113, a limiting rod 12, a controller 13, a supporting leg 14, a driving motor control button 131, an oxygen concentration meter display screen 132, a box cover 2, an air inlet 21, an air outlet 22, a culture box 3, a rotating shaft 31, a stirring rod 311, a spherical head 311a, a driving tooth 312, a tooth groove 313, a bearing 32, a driving motor 33 and a limiting hole 34.

Detailed Description

For a better understanding of the present invention, embodiments thereof are explained in detail below with reference to the accompanying drawings.

The cell hypoxia culture device shown in the attached drawings 1-2 comprises an oxygen concentration meter and a box body 1, wherein the oxygen concentration meter is used for detecting the oxygen concentration in the culture device, a detection head of the oxygen concentration meter is positioned in the box body 1, a sealed box cover 2 is arranged at the upper end of the box body 1, the cell hypoxia culture device can be connected in a threaded connection or covering mode, and a cover body and a cup body connection mode of a vacuum cup can be adopted. A plurality of culture boxes 3 are arranged in the box body 1 side by side, three culture boxes are shown in the attached drawings, and a plurality of culture boxes can be actually arranged as required. The axis of each culture box 3 is provided with a rotating shaft 31, the culture box 3 is internally provided with an annular groove, the rotating shaft 31 is sleeved with a bearing 32 (as shown in fig. 2, for illustration, the lowest rotating shaft does not show the culture box 3, the lowest rotating shaft 31 does not show the bearing 32 and the culture box 3 in fig. 1), the inner ring of the bearing 32 is fixedly connected with the rotating shaft 31, and the outer ring of the bearing 32 is fixedly connected with the culture box 3.

The rotating shaft 31 is provided with a stirring rod 311, and the end part of the stirring rod 311 extends into the culture box 3; the bottom of the stirring rod 311 is provided with a spherical head 311 a. The upper end of the rotating shaft 31 is provided with a driving tooth 312, and the bottom end is provided with a tooth slot 313 matched with the driving tooth 312. The rotating shaft 31 at the lowest end is connected with a driving motor 33, the driving motor 33 is fixed at the bottom end of the box body 1, an output shaft of the driving motor 33 penetrates through a through hole (the diameter of the output shaft is larger than that of the driving motor 33, or a bearing is arranged between the output shaft and the through hole) at the bottom of the box body 1 and then vertically extends upwards into the box body 1, and driving teeth matched with the tooth grooves 313 are arranged at the top end of the output shaft. Four legs 14 are fixed on the bottom edge of the box body 1. When the incubator is used, a plurality of culture boxes 3 can be sequentially placed in the incubator body 1, the tooth socket 313 at the bottom end of the rotating shaft 31 of each culture box 3 is sleeved on the driving tooth 312 at the upper end of the other rotating shaft 31 below, and the rotating shafts 31 of the plurality of culture boxes 3 are sequentially connected. Finally, all the rotating shafts 31 are driven to rotate by the driving motor 33 at the bottom end, and are stirred by the stirring rod 311. The side wall and the bottom wall of the box body 1 are provided with heat preservation chambers 11, specifically, interlayers can be arranged in the side wall and the bottom wall of the box body 1, the upper end of each heat preservation chamber 11 is connected with a water inlet 111, the bottom end of each heat preservation chamber is connected with a water outlet 112, and valves 113 are arranged on the water inlets 111 and the water outlets 112. When in use, warm water can be filled into the heat preservation chamber 11 from the water inlet 111 to ensure that the hypoxia culture device is carried out at a proper temperature. As shown in fig. 1, the upper end of the insulating chamber 11 is closed for clarity of illustrating the insulating chamber 11 without showing the connection end of the upper end of the case body to the lid body 2. The connecting end of the upper end of the box body can be the same as the structure of the existing heat preservation water cup, is provided with a threaded opening and is matched with the cover body 2 through threads.

Two limiting rods 12 are arranged on the bottom wall of the box body 1, and a limiting hole 34 is correspondingly arranged on the side wall of each culture box 3. During the use, put spacing hole 34 on the culture box 3 on gag lever post 12 with the cover in proper order, can avoid like this that culture box 3 follows the rotation when pivot 31 rotates. The cover body 2 is provided with an air inlet 21 and an air outlet 22, the air inlet 21 and the air outlet 22 preferably adopt switch valves, and the air inlet 21 and the air outlet 22 are respectively connected with an air suction pump and a gas injection pump.

The controller 13 is disposed on the sidewall of the box 1, the controller 13 is disposed with a driving motor control button 131 and a display screen 132, and the driving motor 33 can be started or stopped by the driving motor control button 131. The oxygen concentration monitored by the oxygen concentration meter may be displayed on the display screen 132.

When in use, the culture boxes 3 are singly stacked in the box body 1 according to the required quantity, and the culture boxes 3 are taken down one by one from top to bottom when taken out.

Claims

1. Cell hypoxia culture apparatus, it includes box, controller and oxygen concentration table, the upper end of box is provided with case lid, its characterized in that: a plurality of culture boxes are arranged in the box body up and down, a rotating shaft is arranged on the axis of each culture box, a stirring rod is arranged on the rotating shaft, and the end part of the stirring rod extends into the culture boxes; the upper end of the rotating shaft is provided with driving teeth, and the bottom end of the rotating shaft is provided with tooth grooves matched with the driving teeth; the rotating shaft at the lowest end is connected with a driving motor; the side wall and the bottom wall of the box body are provided with heat preservation chambers, the upper end of each heat preservation chamber is connected with a water inlet, the bottom end of each heat preservation chamber is connected with a water outlet, and valves are arranged on the water inlets and the water outlets;

two limiting rods are arranged on the bottom wall of the box body, and a limiting hole is correspondingly arranged on the side wall of each culture box; an air inlet and an air outlet are arranged on the box cover.

2. A cell hypoxia culture apparatus according to claim 1, wherein: the culture box is internally provided with an annular groove; the rotating shaft is sleeved with a bearing, the inner ring of the bearing is fixedly connected with the rotating shaft, and the outer ring of the bearing is fixedly connected with the culture box.

3. A cell hypoxia culture apparatus according to claim 1, wherein: the bottom of the stirring rod is provided with a spherical head.

4. A cell hypoxia culture apparatus according to claim 1, wherein: and the controller is provided with a drive motor control button and a display screen.