CN211689087U

CN211689087U - High-flux genome DNA extraction device

Info

Publication number: CN211689087U
Application number: CN202020045065.9U
Authority: CN
Inventors: 张馨洁; 陈瑞; 张书翠
Original assignee: Shandong Longtai Biotech Co ltd
Current assignee: Shandong Longtai Biotech Co ltd
Priority date: 2020-01-10
Filing date: 2020-01-10
Publication date: 2020-10-16
Anticipated expiration: 2030-01-10

Abstract

The utility model discloses a high flux genome DNA extraction element, including the case main part, the fixed top that is provided with in lower part of case main part, the fixed sill that is provided with in upper portion of case main part, and go up top and sill and correspond from top to bottom, the middle part rigidity of case main part sets up to cavity, the last fixed surface who goes up the top is provided with ultrasonic manufacturing module, the fixed motor that is provided with in inside central point rigidity of sill, the braking end of motor runs through the lower fixed surface bull stick that is connected with of sill, and the lower extreme of bull stick rotates the upper surface middle part position of connecting at the top, the pole body lower part external surface fixed connection of bull stick has the rack, the internal surface fixed of rack has placed and has placed the box. The utility model discloses in, adopt the ultrasonic wave to realize that the material smashes the operation, broken weak point consuming time increases broken speed, and upper portion is provided with the motor, can realize the centrifugal operation of broken liquid on the whole rack, increases operation speed, and centrifugal operation is high-efficient, realizes the high flux purpose.

Description

High-flux genome DNA extraction device

Technical Field

The utility model relates to a DNA draws the field, especially relates to a high flux genome DNA extraction element.

Background

Genomic DNA, which is a carrier of genetic information and the most basic genetic material, plays an important role in genetic variation, metabolic regulation, and the like, and is a major research object in molecular biology and genetic engineering. The plant genome DNA is used as a template of PCR reaction for gene cloning, positioning, molecular marker assisted breeding, transgenic material detection and other researches. Whether the research on the structure and function of plant DNA or the research on the transformation and transduction of exogenous DNA is carried out, the first thing is to extract natural high molecular weight DNA from plant tissues, so that the extraction of sufficient high quality DNA is a prerequisite and necessary guarantee for obtaining accurate experimental results.

The existing DNA extraction process mainly comprises grinding, reagent adding and centrifugal operation, and reagents are added in the extraction process in various types, so that the operation flow speed is too low, one or more tubes can only commonly work together, the grinding process is complicated, the grinding degree can not be guaranteed to be the same, the grinding speed is low, the operation speed is influenced, and the operation efficiency can also be influenced by separating single test tubes one by one.

Disclosure of Invention

The utility model aims at solving the defects existing in the prior art and providing a high-throughput genome DNA extraction device.

In order to achieve the above purpose, the utility model adopts the following technical scheme: the utility model provides a high flux genome DNA extraction element, includes the case main part, the fixed top that is provided with in lower part of case main part, the fixed base station that is provided with in upper portion of case main part, and go up top and base station and correspond from top to bottom, the middle part rigidity of case main part sets up to cavity, the last fixed surface who goes up the top is provided with the ultrasonic manufacturing module, the fixed motor that is provided with in inside central point position of base station, the lower fixed surface who runs through the base station of braking end of motor is connected with the bull stick, and the lower extreme of bull stick rotates the upper surface middle part position of connection at the top, the pole body lower part external surface fixedly connected with rack of bull stick, the internal fixed surface of rack has placed and places the box, the last fixed surface who places the box is provided with the extraction tank, the inside fixed electrothermal.

As a further description of the above technical solution:

the temperature control device is characterized in that a control panel is fixedly arranged on one side of the front surface of the base table, and a temperature display is fixedly arranged on the other side of the front surface of the base table.

As a further description of the above technical solution:

the box comprises a box body and is characterized in that two sides of the middle position of the front surface of the box body are respectively and rotatably connected with a glass door switch, the glass door switches are symmetrically arranged on two sides of the middle position of the front surface of the box body, and the glass door switches are rotatably connected with side plates of the box body through hinges.

As a further description of the above technical solution:

the rack is fixed to be set up to the circular frame, four placing boxes can be placed in the rack, and four placing box side walls are mutually attached, and the extraction groove is fixedly provided with a plurality of upper surfaces for placing boxes.

As a further description of the above technical solution:

the upper surface of rack is provided with the apron, the apron is fixed to be provided with four, and four apron and four place the corresponding setting of box.

As a further description of the above technical solution:

the side plates of the box main body are provided with a certain thickness, and electric heating tubes are arranged inside the plate bodies on the two sides of the box main body.

The utility model discloses following beneficial effect has:

1. the utility model discloses the structure adopts the ultrasonic wave to realize smashing the operation of material, and broken complete and weak point consuming time increases broken speed, and upper portion is provided with the motor, can realize the centrifugal operation of broken liquid on the whole rack, increases operation speed, and centrifugal operation is high-efficient, realizes the purpose of high flux.

2. The utility model discloses can place a plurality of boxes of placing on the structure rack, place the box body upper surface and be provided with a plurality of material standing grooves, can realize a plurality of materials simultaneously and draw the DNA operation, increase the operation speed, can provide the contrast to and the high flux operation.

3. The utility model discloses the fixed electrothermal tube that is provided with of structure both sides plate body inside can realize temperature control through control panel to provide the suitable temperature of different chemical agent reactions, thereby the better flow of DNA is drawed in the realization.

Drawings

FIG. 1 is a front view of a high throughput genomic DNA extraction apparatus;

FIG. 2 is a front sectional view of a high throughput genomic DNA extraction apparatus;

FIG. 3 is a top view of a rack of a high throughput genomic DNA extraction apparatus.

Illustration of the drawings:

1. a box main body; 2. jacking; 3. a base table; 4. opening and closing the glass door; 5. a control panel; 6. a temperature display; 7. a motor; 8. a rotating rod; 9. placing a rack; 10. a cover plate; 11. an electric heating tube; 12. an ultrasonic manufacturing module; 13. placing the box; 14. and (4) an extraction groove.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention; the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance, and furthermore, unless otherwise explicitly stated or limited, the terms "mounted," "connected," and "connected" are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Referring to fig. 1-3, the present invention provides an embodiment: a high-flux genome DNA extraction device comprises a box main body 1, wherein an upper top 2 is fixedly arranged at the lower part of the box main body 1, an upper part connecting position is provided, a bottom table 3 is fixedly arranged at the upper part of the box main body 1, bottom supporting operation is provided, the upper top 2 corresponds to the bottom table 3 up and down, the middle position of the box main body 1 is fixedly arranged to be hollow, an extraction space is provided, an ultrasonic manufacturing module 12 is fixedly arranged on the upper surface of the upper top 2, the effects of ultrasonic vibration and accelerated mixing are realized, a motor 7 is fixedly arranged at the inner central position of the bottom table 3, centrifugal braking is provided, a braking end of the motor 7 penetrates through the lower surface of the bottom table 3 and is fixedly connected with a rotating rod 8, the lower end of the rotating rod 8 is rotatably connected at the middle position of the upper surface of the upper top 2, a placing frame 9 is fixedly connected to the outer surface of the lower part of, an extraction groove 14 is fixedly arranged on the upper surface of the placing box 13 to provide an extraction placing space, and an electric heating tube 11 is fixedly arranged inside a side plate at the middle part of the box main body 1 to realize the control of the internal temperature.

A control panel 5 is fixedly arranged on one side of the front surface of the base 3 to provide integral rhythm control, a temperature display 6 is fixedly arranged on the other side of the front surface of the base 3 to realize temperature display, switch glass doors 4 are respectively and rotatably connected to two sides of the middle position of the front surface of the box main body 1, the switch glass doors 4 are symmetrically arranged on two sides, the switch glass doors 4 are rotatably connected with side plates of the box main body 1 through hinges to provide a visual operation process, a rotating rod 8 is arranged at the center of the upper and lower surfaces of the middle part of the box main body 1, the lower end of the rotating rod 8 is rotatably connected with the middle part of the upper surface of the upper top 2, the central position of an ultrasonic manufacturing module 12 is hollow to provide a switching space of the rotating rod 8, a circular frame is fixedly arranged on a placing frame 9, four placing boxes 13 can be placed in the placing frame 9, the side walls of the four placing boxes 13 are mutually, can draw multiunit DNA simultaneously, the upper surface of rack 9 is provided with apron 10, and apron 10 is fixed to be provided with four, and four apron 10 and four place the corresponding setting of box 13, and liquid can not fly out when guaranteeing centrifugal operation, and the curb plate of case main part 1 is provided with certain thickness, for the electrothermal tube 11 connection provides the prerequisite, and the both sides board of case main part 1 is inside all to be provided with electrothermal tube 11.

The working principle is as follows: when DNA extraction operation is carried out, substances can be placed in the extraction groove 14, the placing box 13 is placed in the placing rack 9, extracting solution is added into the extraction groove 14 in which the substances are placed, the cover plate 10 is covered, the crushing and extracting solution mixing operation of the substances is realized through the ultrasonic manufacturing module 12, when other steps are carried out, different types of extracting solution are added into the extraction groove 14, the centrifugal operation is realized through the starting motor 7, in order to provide a better reaction environment, the electric heating tube 11 is arranged in the box main body 1, reaction proper temperatures of different extracting solutions can be provided, the operation efficiency is increased, high-flux extraction operation is realized, and the DNA extraction process is completed.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.

Claims

1. A high throughput genome DNA extraction device, includes case main part (1), its characterized in that: the lower part of the box main body (1) is fixedly provided with an upper top (2), the upper part of the box main body (1) is fixedly provided with a bottom platform (3), the upper top (2) and the bottom platform (3) correspond up and down, the middle position of the box main body (1) is fixedly arranged to be hollow, the upper surface of the upper top (2) is fixedly provided with an ultrasonic manufacturing module (12), the inner center position of the bottom platform (3) is fixedly provided with a motor (7), the braking end of the motor (7) penetrates through the lower surface of the bottom platform (3) and is fixedly connected with a rotating rod (8), the lower end of the rotating rod (8) is rotatably connected with the middle position of the upper surface of the upper top (2), the outer surface of the lower part of the rod body of the rotating rod (8) is fixedly connected with a placing rack (9), the inner surface of the placing rack (9) is fixedly provided with a placing box (13), and the upper surface of, an electric heating pipe (11) is fixedly arranged inside a side plate at the middle part of the box main body (1).

2. The high-throughput genomic DNA extraction device according to claim 1, wherein: the temperature control device is characterized in that a control plate (5) is fixedly arranged on one side of the front surface of the base table (3), and a temperature display (6) is fixedly arranged on the other side of the front surface of the base table (3).

3. The high-throughput genomic DNA extraction device according to claim 1, wherein: the box is characterized in that two sides of the middle position of the front surface of the box main body (1) are respectively and rotatably connected with a switch glass door (4), the switch glass doors (4) are symmetrically arranged, and the switch glass doors (4) are rotatably connected with side plates of the box main body (1) through hinges.

4. The high-throughput genomic DNA extraction device according to claim 1, wherein: the ultrasonic wave manufacturing device is characterized in that the rotating rod (8) is arranged at the center of the upper surface and the lower surface of the middle part of the box main body (1), the lower end of the rotating rod (8) is rotatably connected to the middle part of the upper surface of the upper top (2), and the central position of the ultrasonic wave manufacturing module (12) is arranged to be hollow.

5. The high-throughput genomic DNA extraction device according to claim 1, wherein: the rack (9) is fixedly arranged to be a round frame, four placing boxes (13) can be placed in the rack (9), the side walls of the four placing boxes (13) are attached to each other, and a plurality of extracting grooves (14) are fixedly arranged on the upper surface of the placing boxes (13).

6. The high-throughput genomic DNA extraction device according to claim 1, wherein: the upper surface of rack (9) is provided with apron (10), apron (10) are fixed to be provided with four, and four apron (10) and four place box (13) and correspond the setting.

7. The high-throughput genomic DNA extraction device according to claim 1, wherein: the side plates of the box main body (1) are provided with a certain thickness, and electric heating tubes (11) are arranged inside the plate bodies on the two sides of the box main body (1).