CN211645237U - Sample protection device with microorganisms - Google Patents
Sample protection device with microorganisms Download PDFInfo
- Publication number
- CN211645237U CN211645237U CN201922224013.8U CN201922224013U CN211645237U CN 211645237 U CN211645237 U CN 211645237U CN 201922224013 U CN201922224013 U CN 201922224013U CN 211645237 U CN211645237 U CN 211645237U
- Authority
- CN
- China
- Prior art keywords
- sealing
- cover body
- hole
- piece
- accommodating cavity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Sampling And Sample Adjustment (AREA)
Abstract
The utility model provides a carry sample protection device of microorganism realizes protecting the sample of gathering, realizes the save of sample normal atmospheric temperature. The device, comprising: a storage tube; with the tube cap of storage tube detachable connection, the tube cap includes: a cover body; the annular bulge is fixed on the inner side wall of the cover body; the sealing sheet is attached to the annular bulge, the sealing sheet, the annular bulge and the cover body jointly enclose an accommodating cavity for forming sealing protection liquid, and a gap for the protection liquid to pass through is formed between the sealing sheet and the inner side wall of the cover body; the operating piece partially penetrates through a through hole formed in the cover body and then extends into the accommodating cavity; and a notch for flowing out of the protection liquid in the accommodating cavity is formed between the sealing sheet and the annular bulge by actuating the operating piece.
Description
Technical Field
The utility model relates to a medical science sample acquisition and storage field specifically are a carry sample protection device of microorganism.
Background
The intestinal flora is the most important external environment in human body, and various microorganisms are combined according to a certain proportion, are mutually restricted and mutually dependent, and form an ecological balance in quality and quantity. The majority of the fecal bacteria are anaerobes, some of which are strictly anaerobes, which easily lose their activity in aerobic environments. In the traditional method, bacteria are separated and cultured by using a solid or liquid culture medium, the period is long, all intestinal flora is difficult to separate and culture due to technical influence, and the research of people on the intestinal flora is limited. At present, the NGS high-throughput sequencing technology is adopted, separation culture is not needed, and the types and the number of the intestinal flora of the human body can be comprehensively detected by extracting and detecting DNA of a fecal sample.
Due to the instability of microorganism samples and the complexity of sample preservation, the normal temperature preservation and collection of microorganisms are a common concern. Therefore, the preservation of fecal samples is more critical than the protection of other bacteria.
SUMMERY OF THE UTILITY MODEL
The utility model provides a carry sample protection device of microorganism realizes protecting the sample of gathering, realizes the save of sample normal atmospheric temperature.
The technical scheme of the utility model is that:
the utility model provides a carry sample protection device of microorganism, include: a storage tube; with the tube cap of storage tube detachable connection, the tube cap includes: a cover body;
the annular bulge is fixed on the inner side wall of the cover body;
the sealing sheet is attached to the annular bulge, the sealing sheet, the annular bulge and the cover body jointly enclose an accommodating cavity for forming sealing protection liquid, and a gap for the protection liquid to pass through is formed between the sealing sheet and the inner side wall of the cover body;
the operating piece partially penetrates through a through hole formed in the cover body and then extends into the accommodating cavity;
and a notch for flowing out of the protection liquid in the accommodating cavity is formed between the sealing sheet and the annular bulge by actuating the operating piece.
Preferably, the through hole provided in the cover body includes:
a first hole formed by opening from the outer surface of the top wall of the cover body;
a second hole formed by opening from the inner surface of the top wall of the cover body, the second hole being connected to the first hole; the aperture of the second hole is gradually increased from top to bottom;
the operating member includes:
a button disposed within the first aperture;
the push rod penetrates through the second hole and extends into the accommodating cavity, and the outer diameter of the push rod is gradually increased from top to bottom;
a portion of the push rod and the second bore form an interference fit when the button is not pressed downward.
Preferably, the sealing sheet is attached to the lower side of the annular bulge;
and pushing the sealing sheet to move downwards by pressing the operating piece downwards, so that a notch for flowing out of the protection liquid in the accommodating cavity is formed between the sealing sheet and the annular protrusion.
Preferably, the apparatus further comprises:
the fixing piece is fixed on the inner side wall of the cover body and arranged on the lower side of the sealing sheet, and a channel for the protective liquid to flow into the storage pipe is arranged on the fixing piece;
a spring connected between the sealing plate and the fixing member;
the spring is in a compressed state when the sealing plate is pushed down by the operating member.
Preferably, the sealing sheet is attached to the upper side of the annular protrusion, and the operating piece is connected with the annular protrusion;
through upwards pulling operating parts, drive the gasket rebound, make the gasket with form the confession between the annular arch the breach that the protection liquid in the holding intracavity flows.
Preferably, the apparatus further comprises:
the fixing piece is fixed on the inner side wall of the cover body and arranged on the lower side of the annular bulge, and a channel for the protective liquid to flow into the storage pipe is formed in the fixing piece;
a spring connected between the sealing plate and the fixing member;
the spring is in tension when the sealing plate is pushed down by the operating member.
Preferably, a guide slope is formed on the upper surface of the fixed part facing the sealing sheet, and the guide slope is communicated to a channel on the upper surface of the fixed part.
Preferably, the apparatus further comprises:
and the collector is fixed on the fixing piece, and the collector part extends into the storage pipe.
The utility model has the advantages that:
the sample stored by the protective solution can be transported and stored at normal temperature, so that the cost of low-temperature transportation and storage is saved. The utility model discloses wide application in collection, normal atmospheric temperature transportation, the low temperature transportation of microorganism sample, convenient to carry, simple to use, realization normal atmospheric temperature microorganism sample preserve the transportation.
Drawings
Fig. 1 is a schematic structural diagram of a first embodiment of the present invention;
fig. 2 is a schematic view showing leakage of the protective liquid in the first embodiment of the present invention;
FIG. 3 is an enlarged schematic view of FIG. 1 at A;
FIG. 4 is an enlarged view of FIG. 2 at B;
fig. 5 is a schematic view of a sample bubble in a protective liquid according to a first embodiment of the present invention;
fig. 6 is a schematic structural diagram of a second embodiment of the present invention;
description of reference numerals: 11. a cover body; 111. a first hole; 112. a second hole; 12. an annular projection; 13. sealing the sheet; 14. an operating member; 141. a button; 142. a push rod; 15. a fixing member; 16. a spring; 17. a collector; 18. an internal thread; 2. a storage tube; 21. an external thread; 3. a sample; 101. an accommodating cavity; 102. a protective solution; 103. a gap; 104. and (4) a notch.
Detailed Description
Exemplary embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. While exemplary embodiments of the invention are shown in the drawings, it should be understood that the invention can be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.
Referring to fig. 1 to 5, the present invention provides a sample protection device carrying microorganisms, including: a storage tube 2; with the tube cap of storage tube 2 detachable connection, the tube cap includes: the cover body 11 is provided with an internal thread 18 on the cover body 11, an external thread 21 on the storage tube 2, and the cover body 11 and the storage tube 2 are screwed together by matching the internal thread 18 with the external thread 21; an annular protrusion 12 fixed to an inner sidewall of the cover body 11; a sealing sheet 13 attached to the annular protrusion 12, wherein the sealing sheet 13, the annular protrusion 12 and the cover 11 together enclose an accommodating cavity 101 for forming a sealing protection liquid 102, and a gap 103 for the protection liquid 102 to pass through is formed between the sealing sheet 13 and the inner side wall of the cover 11; the operating part 14, part of which passes through the through hole arranged on the cover body 11 and then extends into the accommodating cavity 101; by operating the operating element 14, a gap 104 through which the protection liquid 102 in the housing chamber 101 flows out is formed between the seal piece 13 and the annular protrusion 12.
As shown in fig. 1 to 5, the cover 11 specifically includes a top wall and a side wall, and when the operating element 14 is not operated, an accommodating cavity 101 defined by the sealing sheet 13, the annular protrusion 12 and the inner side wall of the cover 11 is a sealed cavity, so that the protective liquid 102 is sealed in the accommodating cavity 101.
As shown in fig. 1 to 6, a through hole communicating with the accommodating cavity 101 is provided on the top wall of the cover 11, and a portion of the operation member 14 slidably passes through the through hole from the outside of the cover 11 and extends into the accommodating cavity 101. In order to achieve that the operating element 14 can slide relative to the cover 11 and that the sealing liquid does not leak through the through hole, various implementations are provided in the present embodiment.
In the embodiment of attaching the sealing sheet 13 to the upper surface of the annular protrusion 12, as shown in fig. 2 and 4, the through hole formed in the top wall of the lid 11 is formed by a first hole 111 and a second hole 112 connected to each other, the first hole 111 is formed by opening a hole in the outer surface of the top wall of the lid 11, the second hole 112 is formed by opening a hole in the inner surface of the top wall of the lid 11, and the diameter of the second hole 112 gradually increases from top to bottom. The operating element 14 includes a button 141 for pressing and a push rod 142 connected to the button 141, the button 141 is disposed in the first hole 111, the button 141 is hidden in the first hole 111 to improve the aesthetic appearance of the structure, and a gap 103 is formed between the button 141 and the first hole 111. The outer diameter of the connecting rod is gradually increased from top to bottom. When the button 141 is not pressed, an interference fit, and thus a seal, is formed between a portion of the push rod 142 and the second hole 112 to prevent the protective liquid 102 from leaking. When the protection liquid 102 needs to be fed into the storage tube 2, and an operator only needs to press the button 141, under the action of pressure, the push rod 142 moves downwards, and the push rod 142 further pushes the sealing sheet 13 to move downwards, so that the protection liquid 102 flows out; meanwhile, since the outer diameter of the push rod 142 is gradually increased from top to bottom, after the portion of the push rod 142 that forms the interference fit with the second hole 112 passes through the second hole 112, the push rod 142 can smoothly move downward through the second hole 112. Meanwhile, since the interference fit is formed between the push rod 142 and the second hole 112, the push rod 142 needs to be pushed to move downwards by applying a large pressure by an operator, so that the operator can be prevented from mistakenly touching the button 141. In another implementation, the button 141 of the operating element 14 protrudes outside the outer surface of the top wall of the cover 11, and the inner diameter of a through hole formed in the cover 11 gradually increases from top to bottom, and the through hole forms an interference fit with a portion of the push rod 142 when the button 141 is not pressed.
In the case where the sealing sheet 13 is attached to the lower surface of the annular protrusion 12, as shown in fig. 6, the button 141 of the operating element 14 protrudes outside the outer surface of the top wall of the lid body 11, the inner diameter of a through hole formed in the lid body 11 is gradually reduced from top to bottom, the outer diameter of the push rod 142 of the operating element 14 is gradually reduced from top to bottom, and when the button 141 is not pulled upward, the through hole is integrally in interference fit with a portion of the push rod 142.
In this embodiment, 2 schemes are provided to realize that the operating part 14 drives the sealing piece 13 to move to form the notch 104. As shown in fig. 1 to 5, in a first implementation, the sealing sheet 13 is attached to the lower side of the annular protrusion 12; by pressing the operating part 14 downwards, the sealing sheet 13 is pushed to move downwards, so that a gap 104 for the protective liquid 102 in the accommodating cavity 101 to flow out is formed between the sealing sheet 13 and the annular protrusion 12. At this time, the sealing piece 13 passes through and forms the gap 104 with the lower surface of the annular protrusion 12. Since the operating member 14 does not have a self-return function, in view of the risk of leakage of the return fluid entering the storage tube through the through hole during storage of the sample 3, in this first implementation the device further comprises: a fixing member 15 fixed to an inner side wall of the cover 11, the fixing member 15 being disposed under the sealing sheet 13, the fixing member 15 being provided with a passage through which the protective liquid 102 flows into the storage tube 2; a spring 16 connected between the sealing plate 13 and the fixing member 15; the spring 16 is in a compressed state when the sealing plate 13 is pushed down by the operating member 14. When the button 141 of the operating member 14 is pressed down, the push rod 142 of the operating member 14 pushes the sealing sheet 13 to move down, a gap 104 is formed between the sealing sheet 13 and the lower surface of the annular protrusion 12, and the spring 16 is compressed when the sealing sheet 13 moves down because the spring 16 and the sealing sheet 13 are connected together. When an operator stops pressing the button 141 of the operating part 14, the compressed spring 16 rebounds to reversely push the sealing sheet 13 to move upwards, the sealing sheet 13 further drives the push rod 142 to reversely move upwards, the push rod 142 and the sealing sheet 13 both reset, sealing is formed between the sealing sheet 13 and the annular protrusion 12, and backflow of backflow liquid entering the storage tank can be prevented.
In a second realization, the above-mentioned gap 104 is formed between the sealing disc 13 and the upper surface of the annular projection 12. As shown in fig. 6, the sealing plate 13 is attached to the upper side of the annular protrusion 12, and the operating element 14 is connected to the annular protrusion 12; by pulling the operating part 14 upwards, the sealing sheet 13 is driven to move upwards, so that a gap 104 for the protective liquid 102 in the accommodating cavity 101 to flow out is formed between the sealing sheet 13 and the annular protrusion 12. At this time, the sealing piece 13 passes through and forms the gap 104 with the lower surface of the annular protrusion 12. Since the operating member 14 does not have a self-return function, in view of the risk of leakage of the return fluid entering the storage tube through the through hole during storage of the sample 3, in this second implementation the device further comprises: a fixing member 15 fixed on the inner side wall of the cover 11, wherein the fixing member 15 is arranged at the lower side of the annular protrusion 12, and a channel for the protective liquid 102 to flow into the storage pipe 2 is arranged on the fixing member 15; a spring 16 connected between the sealing plate 13 and the fixing member 15; when the sealing plate 13 is pushed down by the operating member 14, the spring 16 is in a stretched state. When the button 141 of the operating member 14 is pulled up, the push rod 142 of the operating member 14 pulls the sealing sheet 13 upwards, a gap 104 is formed between the sealing sheet 13 and the upper surface of the annular protrusion 12, and the spring 16 is stretched when the sealing sheet 13 moves upwards because the spring 16 and the sealing sheet 13 are connected together. When the operator stops pressing the button 141 of the operating element 14, the stretched spring 16 rebounds to reversely push the sealing sheet 13 to move upwards, the sealing sheet 13 further drives the push rod 142 to move downwards reversely, the push rod 142 and the sealing sheet 13 both reset, and a seal is formed between the sealing sheet 13 and the annular protrusion 12, so that backflow of backflow liquid entering the storage tank can be prevented.
For both solutions in fig. 1 to 6, a guide bevel is formed on the upper surface of the fixing element 15 facing the sealing plate 13, said guide bevel being connected to a passage on the upper surface of the fixing element 15. The guide slope is mainly used to guide the protective liquid 102 flowing onto the upper surface of the fixing member 15, so that the protective liquid 102 can rapidly flow into the storage pipe 2.
For both versions of fig. 1 to 6, the apparatus further comprises:
a collector 17 fixed on the fixing member 15, wherein the collector 17 partially extends into the storage tube 2. Wherein, the lower extreme of collector 17 is the spoon form for carry out sample 3 and gather. In the invention, taking feces as an example, the collection and storage of the feces sample 3 are carried out according to the following steps:
step 1, an operator takes out the device of the embodiment, screws off the pipe cover in the forward direction, and collects a small amount of excrement from the excrement collecting box by means of a collector 17 in the pipe cover;
step 3, pressing or upwards pulling the operating part 14 to enable the operating part 14 to drive the sealing sheet 13 and the annular protrusion 12 to form a gap 104, enabling the protective liquid 102 stored in the accommodating space to flow into the storage pipe 2 through the gap 104, the gap 103 and a channel on the fixing part 15, soaking the excrement sample 3 stored in the storage pipe 2, and protecting the excrement sample 3;
step 4, releasing the operating element 14, and returning the sealing sheet 13 and the operating element 14 under the reaction of the spring 16.
In addition, the device provided in the present application can be used for collecting and storing the stool sample 3, and can also be used for storing other samples 3 carrying microorganisms, such as samples 3 of oral tissues and the like. The protective solution 102 may be composed of methanol, ethanol, and β -mercaptoethanol.
The sample 3 stored by the protective solution 102 can be transported and stored under normal temperature, so that the cost of low-temperature transportation and storage is saved. The utility model discloses wide application in collection, normal atmospheric temperature transportation, the low temperature transportation of microorganism sample 3, convenient to carry, simple to use, realization normal atmospheric temperature microorganism sample 3 preserve the transportation.
The foregoing embodiments have described only some of the one or more embodiments of the present invention, but it will be appreciated by those skilled in the art that the invention can be embodied in many other forms without departing from the spirit or scope of the invention. Accordingly, the present examples and embodiments are to be considered as illustrative and not restrictive, and various modifications and substitutions may be made thereto without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (8)
1. A microorganism-bearing sample protection device, comprising: a storage tube (2); a tube cap detachably connected to the storage tube (2), the tube cap comprising: a cover (11);
an annular protrusion (12) fixed on the inner side wall of the cover body (11);
the sealing piece (13) is arranged in a fit mode with the annular bulge (12), the sealing piece (13), the annular bulge (12) and the cover body (11) jointly enclose an accommodating cavity (101) for forming sealing protection liquid (102), and a gap (103) for the protection liquid (102) to pass through is formed between the sealing piece (13) and the inner side wall of the cover body (11);
the operating piece (14) partially penetrates through a through hole formed in the cover body (11) and then extends into the accommodating cavity (101);
and a notch (104) for the protective liquid (102) in the accommodating cavity (101) to flow out is formed between the sealing sheet (13) and the annular bulge (12) by operating the operating piece (14).
2. Device according to claim 1, characterized in that said sealing disc (13) is applied on the lower side of said annular projection (12);
by pressing the operating piece (14) downwards, the sealing sheet (13) is pushed to move downwards, and a gap (104) for the protective liquid (102) in the accommodating cavity (101) to flow out is formed between the sealing sheet (13) and the annular protrusion (12).
3. The apparatus of claim 2, further comprising:
the fixing piece (15) is fixed on the inner side wall of the cover body (11), the fixing piece (15) is arranged on the lower side of the sealing sheet (13), and a channel for the protective liquid (102) to flow into the storage pipe (2) is arranged on the fixing piece (15);
a spring (16) connected between the sealing plate (13) and the fixing piece (15);
the spring (16) is in a compressed state when the sealing plate (13) is pushed down by the operating member (14).
4. The device according to claim 3, characterized in that the through hole provided on the cover (11) comprises:
a first hole (111) formed by opening from the outer surface of the top wall of the cover body (11);
a second hole (112) opened from an inner surface of a top wall of the cover body (11), the second hole (112) being connected to the first hole (111); the aperture of the second hole (112) is gradually increased from top to bottom;
the operating member (14) includes:
a button (141) disposed within the first aperture (111);
the push rod (142) penetrates through the second hole (112) and extends into the accommodating cavity (101), and the outer diameter of the push rod (142) is gradually increased from top to bottom;
a portion of the push rod (142) and the second hole (112) form an interference fit when the button (141) is not pressed downward.
5. The device according to claim 1, characterized in that said sealing plate (13) is applied on the upper side of said annular projection (12) and said operating element (14) is associated with said annular projection (12);
the operating piece (14) is pulled upwards to drive the sealing piece (13) to move upwards, so that a gap (104) for flowing out of the protection liquid (102) in the accommodating cavity (101) is formed between the sealing piece (13) and the annular protrusion (12).
6. The apparatus of claim 5, further comprising:
the fixing piece (15) is fixed on the inner side wall of the cover body (11), the fixing piece (15) is arranged on the lower side of the annular bulge (12), and a channel for the protective liquid (102) to flow into the storage pipe (2) is formed in the fixing piece (15);
a spring (16) connected between the sealing plate (13) and the fixing piece (15);
the spring (16) is in a stretched state when the sealing plate (13) is pushed down by the operating member (14).
7. The device according to claim 4 or 6, characterized in that the upper surface of the stationary part (15) facing the sealing plate (13) is formed with a guiding bevel, which communicates to a passage on the upper surface of the stationary part (15).
8. The apparatus of claim 4 or 6, further comprising:
a collector (17) fixed on the fixing part (15), wherein part of the collector (17) extends into the storage pipe (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922224013.8U CN211645237U (en) | 2019-12-12 | 2019-12-12 | Sample protection device with microorganisms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922224013.8U CN211645237U (en) | 2019-12-12 | 2019-12-12 | Sample protection device with microorganisms |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211645237U true CN211645237U (en) | 2020-10-09 |
Family
ID=72695771
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201922224013.8U Active CN211645237U (en) | 2019-12-12 | 2019-12-12 | Sample protection device with microorganisms |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211645237U (en) |
-
2019
- 2019-12-12 CN CN201922224013.8U patent/CN211645237U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5015964B2 (en) | Sample container for containing a small amount of liquid for analysis | |
| CN211645237U (en) | Sample protection device with microorganisms | |
| CN102252871B (en) | One-time conveying sampler | |
| CN103114035A (en) | Device for fully-enclosed extraction, transfer and storage of nucleic acids | |
| US3919053A (en) | Assembly for specimen culturing | |
| CN212722096U (en) | Sample collection device | |
| CN217149141U (en) | Single-tube nucleic acid extraction device | |
| CN112683897B (en) | Urinary sediment detection kit | |
| CN214004669U (en) | Biological cell culture device convenient to sample | |
| CN212134189U (en) | Environmental pollution source water quality sampler | |
| CN216445342U (en) | Microbial fermentation is with detecting sample extraction element | |
| CN202126362U (en) | Disposable transportation sampler | |
| CN219348647U (en) | Fecal instant detection device | |
| CN201971832U (en) | Sampling device for fermentation tank | |
| CN218202805U (en) | PCR tube for nucleic acid amplification | |
| CN201524341U (en) | Puncture guide device for cardiovascular intervention therapy | |
| CN215129050U (en) | Isolated medical endocrine sample extractor | |
| CN219951011U (en) | Cell expansion culture dish | |
| CN220270925U (en) | Simple gas sampler capable of being operated by one hand | |
| CN209968375U (en) | Oxygen collection device convenient to operation | |
| CN221091778U (en) | Dampproofing reserves device of organic fertilizer | |
| CN217845711U (en) | Excrement sampling device | |
| CN220671004U (en) | Food inspection sampling device | |
| CN219313518U (en) | Purification kit for nucleic acid extraction | |
| CN218885466U (en) | Push type excrement sampler |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |