CN214004669U - Biological cell culture device convenient to sample - Google Patents
Biological cell culture device convenient to sample Download PDFInfo
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- CN214004669U CN214004669U CN202022856515.5U CN202022856515U CN214004669U CN 214004669 U CN214004669 U CN 214004669U CN 202022856515 U CN202022856515 U CN 202022856515U CN 214004669 U CN214004669 U CN 214004669U
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- sampling
- bottle body
- cell culture
- sleeve
- biological cell
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Abstract
The utility model relates to a cell culture technical field just discloses a biological cell culture device convenient to sample, including the body, the outside intercommunication of body has quantity to be two breather nozzles, the outside intercommunication of body has one end to extend to the inside appearance pipe of body, the inside swing joint that goes out the appearance pipe has one end to extend to the sealing plug in the appearance outside of managing, the sampling hole has been seted up at the top of body, the inside swing joint of body has one end to run through the sampling hole and extends to the sampling tube of body top, the top threaded connection of body has the sleeve that is located the sampling tube outside. This biological cell culture device convenient to sample has solved current cell culture device and its inside culture medium can expose by a large scale in external environment when carrying out sampling operation, leads to the pollution of culture medium easily, and external object when sampling operation enters into the culture dish easily, causes the problem of destruction to the culture medium.
Description
Technical Field
The utility model relates to a cell culture device technical field specifically is a biological cell culture device convenient to sample.
Background
Cell culture, also called cell cloning technology, is also known as cell culture technology in biology, and is an essential process for the whole biotechnology or one of the biological cloning technologies, and cell culture itself is the large-scale cloning of cells, and is usually performed by a culture dish.
The cell culture device that has now on the market mainly is the culture dish, and it has multiple specification, can provide necessary space for cell culture, but the culture medium of current cell culture device inside can expose by a large scale in external environment when carrying out sampling operation, leads to the pollution of culture medium easily, and external object easily enters into the culture dish during sampling operation, causes the destruction to the culture medium, so proposes a biological cell culture device convenient to sample and solves the above-mentioned problem of proposing.
SUMMERY OF THE UTILITY MODEL
Technical problem to be solved
The utility model provides a be not enough to prior art, the utility model provides a biological cell culture device convenient to sample possesses advantages such as the sample of being convenient for, has solved current cell culture device and can expose by a large scale in external environment its inside culture medium when carrying out sampling operation, leads to the pollution of culture medium easily, and external object when sampling operation enters into the culture dish easily, causes the problem of destruction to the culture medium.
(II) technical scheme
For the purpose of realizing the above-mentioned sample of being convenient for, the utility model provides a following technical scheme: a biological cell culture device convenient for sampling comprises a bottle body, wherein the outer side of the bottle body is communicated with two vent nozzles, one end of the vent nozzles extends to a sample outlet pipe inside the bottle body, one end of the interior of the sample outlet pipe is movably connected with a sealing plug, the other end of the interior of the sample outlet pipe extends to the outer side of the sample outlet pipe, a sampling hole is formed in the top of the bottle body, one end of the interior of the bottle body is movably connected with the sampling pipe, the sampling pipe penetrates through the sampling hole and extends to the upper part of the bottle body, the top of the bottle body is in threaded connection with a sleeve positioned on the outer side of the sampling pipe, a sealing sleeve positioned on the outer side of the sampling pipe and one end of the sealing sleeve extends to the upper part of the sleeve is fixedly connected with the outer side of the sealing sleeve, one opposite side of the fixing base and the sleeve is fixedly connected with a spring, the spring is positioned on the outer side of the sealing sleeve, the outer side of the sampling pipe is fixedly connected with an elastic rubber sleeve positioned on the upper part of the sealing sleeve, and one end of the elastic rubber sleeve extends to the upper part of the sampling pipe, the outer side of the elastic rubber sleeve is fixedly connected with a fixing ring, the top of the bottle body is provided with an operation hole located on the right side of the sampling hole, the top of the bottle body is in threaded connection with a cover located above the operation hole, and the inside of the cover is fixedly connected with a rubber film.
Preferably, the body is the glass bottle, go out the appearance pipe and be the slope and arrange, and go out the appearance pipe and be located the inside one end of body and be higher than the one end of keeping away from the body.
Preferably, the sealing plug is a rubber plug, and the left side of the sealing plug is fixedly connected with a spherical handle.
Preferably, two the equal fixedly connected with one end in inside of breather nozzle extends to the air cock stopper on breather nozzle right side, and the air cock stopper is the rubber buffer, the inside of breather nozzle is seted up and is located the air cock stopper outside and with the inside air vent that communicates of body.
Preferably, the sampling tube is funnel-shaped, and the volume of the inner space of the sampling tube is larger than that of the inner space of the elastic rubber sleeve.
Preferably, the sealing sleeve is a tubular rubber film, and the adhesive film is a circular rubber film.
(III) advantageous effects
Compared with the prior art, the utility model provides a biological cell culture device convenient to sample possesses following beneficial effect:
the biological cell culture device convenient for sampling is characterized in that the sampling tube is arranged, a culture medium of the device is loaded through the bottle body, the operation such as liquid adding and the like can be carried out on the interior of the device through the operation hole after the cover is opened, the sampling tube can be jacked up under the action of the spring when not in use, when the sampling operation is required, the sealing plug is pulled out, the elastic rubber sleeve is held and pressed downwards, the sampling tube is inserted into the culture medium in the bottle body, the fixing ring is held to keep the sampling tube in position and appropriately loosen the elastic rubber sleeve, the sampling tube sucks partial sample, then the lower end of the sampling tube is aligned to the right end of the sampling tube, the elastic rubber sleeve is held again to enable the sample in the sampling tube to be extruded out and flow out through the sampling tube, the device can not expose the culture medium in the outside air when the sampling operation is carried out, and the possibility of culture medium pollution is reduced to a certain extent, and the external object is difficult to enter the inside of the bottle body during the sampling operation, thereby achieving the purpose of being convenient for sampling.
Drawings
Fig. 1 is a front view of the present invention;
FIG. 2 is a front cross-sectional view of the present invention;
fig. 3 is an enlarged view of a portion a in fig. 2 according to the present invention.
In the figure: 1. a bottle body; 2. a breather nozzle; 3. a sample outlet pipe; 4. a sealing plug; 5. a sampling hole; 6. a sleeve; 7. a sampling tube; 8. sealing sleeves; 9. a fixed seat; 10. a spring; 11. an elastic rubber sleeve; 12. a fixing ring; 13. an operation hole; 14. a glue film; 15. and a cover.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-3, a biological cell culture device convenient for sampling comprises a bottle body 1, the outer side of the bottle body 1 is communicated with two vent nozzles 2, the inner parts of the two vent nozzles 2 are fixedly connected with air tap plugs, one end of each air tap plug extends to the right side of the vent nozzle 2, the air tap plugs are rubber plugs, the inner parts of the vent nozzles 2 are provided with vent holes which are positioned at the outer sides of the air tap plugs and communicated with the inner parts of the bottle body 1, the outer side of the bottle body 1 is communicated with a sample outlet pipe 3, one end of each sample outlet pipe 3 extends to the inner part of the bottle body 1, the bottle body 1 is a glass bottle, the sample outlet pipe 3 is obliquely arranged, one end of the sample outlet pipe 3 positioned in the bottle body 1 is higher than one end far away from the bottle body 1, the inner part of the sample outlet pipe 3 is movably connected with a sealing plug 4, one end of each sealing plug 4 is a sealing plug, the left side of the sealing plug 4 is fixedly connected with a spherical handle, the top part of the bottle body 1 is provided with a sampling hole 5, the inner part of the bottle body 1 is movably connected with a sampling tube 7, one end of the sampling tube 7 penetrates through the sampling hole 5 and extends to the upper part of the bottle body 1, the sampling tube 7 is in a funnel shape, the volume of the inner space of the sampling tube 7 is larger than that of the inner space of the elastic rubber sleeve 11, the top part of the bottle body 1 is in threaded connection with a sleeve 6 positioned at the outer side of the sampling tube 7, the inner part of the sleeve 6 is fixedly connected with a sealing sleeve 8 positioned at the outer side of the sampling tube 7, one end of the sealing sleeve 8 extends to the upper part of the sleeve 6, the outer side of the sealing sleeve 8 is fixedly connected with a fixed seat 9 positioned at the upper part of the sleeve 6, the opposite sides of the fixed seat 9 and the sleeve 6 are fixedly connected with a spring 10, the spring 10 is positioned at the outer side of the sealing sleeve 8, the outer side of the sampling tube 7 is fixedly connected with an elastic rubber sleeve 11 positioned at the upper part of the sealing sleeve 8, the outer side of the elastic rubber sleeve 11 is fixedly connected with a fixed ring 12, the top part of the bottle body 1 is provided with an operation hole 13 positioned at the right side of the sampling hole 5, the top of the bottle body 1 is in threaded connection with a cover 15 positioned above the operation hole 13, the cover 15 is fixedly connected with an adhesive film 14 inside, the sealing sleeve 8 is a tubular rubber film, and the adhesive film 14 is a circular rubber film.
In summary, in the biological cell culture device convenient for sampling, the sampling tube 7 is arranged, the culture medium of the device is contained in the bottle body 1, the operation such as liquid adding and the like can be carried out on the interior of the device through the operation hole 13 after the cover 15 is opened, the sampling tube 7 can be jacked up under the action of the spring 10 when the device is not used, when the sampling operation is required, the sealing plug 4 is pulled out, the elastic rubber sleeve 11 is pinched and pressed downwards, the sampling tube 7 is inserted into the culture medium in the bottle body 1, the fixing ring 12 is pinched to hold the sampling tube 7 in position and appropriately release the elastic rubber sleeve 11, so that the sampling tube 7 sucks partial sample, then the lower end of the sampling tube 7 is aligned with the right end of the sample tube 3, the elastic rubber sleeve 11 is pinched again, so that the sample in the sampling tube 7 can be extruded and flow out through the sample tube 3, the device can not expose the culture medium in a large area in the outside air when the sampling operation is carried out, the contaminated possibility of culture medium has been reduced to a certain extent, and external object is difficult to enter into the inside of body 1 when sampling operation to reach the purpose of the sample of being convenient for, solved current cell culture device when carrying out sampling operation its inside culture medium can expose by a large scale in external environment, lead to the pollution of culture medium easily, and external object when sampling operation enters into the culture dish easily, causes the problem of destruction to the culture medium.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. A biological cell culture device convenient for sampling comprises a bottle body (1), and is characterized in that: the outer side of the bottle body (1) is communicated with two air vent nozzles (2), the outer side of the bottle body (1) is communicated with a sample outlet pipe (3) with one end extending to the inside of the bottle body (1), the inner part of the sample outlet pipe (3) is movably connected with a sealing plug (4) with one end extending to the outer side of the sample outlet pipe (3), the top of the bottle body (1) is provided with a sampling hole (5), the inner part of the bottle body (1) is movably connected with a sampling pipe (7) with one end penetrating through the sampling hole (5) and extending to the upper part of the bottle body (1), the top of the bottle body (1) is in threaded connection with a sleeve (6) positioned on the outer side of the sampling pipe (7), the inner part of the sleeve (6) is fixedly connected with a sealing sleeve (8) positioned on the outer side of the sampling pipe (7) and one end extending to the upper part of the sleeve (6), the outer side of the sealing sleeve (8) is fixedly connected with a fixing seat (9) positioned on the upper part of the sleeve (6), the utility model discloses a sampling device, including fixing base (9), spring (10), sampling tube (7), fixed ring (12), operating hole (13) that are located sampling hole (5) right side are seted up at the top of body (1), the top threaded connection of body (1) has lid (15) that are located operating hole (13) top, the inside fixedly connected with glued membrane (14) of lid (15).
2. A biological cell culture apparatus for facilitating sampling according to claim 1, wherein: the bottle body (1) is a glass bottle, the sample outlet pipe (3) is obliquely arranged, and one end of the sample outlet pipe (3) located inside the bottle body (1) is higher than one end of the sample outlet pipe far away from the bottle body (1).
3. A biological cell culture apparatus for facilitating sampling according to claim 1, wherein: the sealing plug (4) is a rubber plug, and the left side of the sealing plug (4) is fixedly connected with a spherical handle.
4. A biological cell culture apparatus for facilitating sampling according to claim 1, wherein: two the equal fixedly connected with one end in inside of breather nozzle (2) extends to the air cock stopper on breather nozzle (2) right side, and the air cock stopper is the rubber buffer, the inside of breather nozzle (2) is seted up and is located the air cock stopper outside and with the inside air vent that communicates of body (1).
5. A biological cell culture apparatus for facilitating sampling according to claim 1, wherein: sampling tube (7) are the funnel shape, and the volume of sampling tube (7) inner space is greater than the volume of elasticity gum cover (11) inner space.
6. A biological cell culture apparatus for facilitating sampling according to claim 1, wherein: the sealing sleeve (8) is a tubular rubber film, and the adhesive film (14) is a circular rubber film.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022856515.5U CN214004669U (en) | 2020-12-02 | 2020-12-02 | Biological cell culture device convenient to sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022856515.5U CN214004669U (en) | 2020-12-02 | 2020-12-02 | Biological cell culture device convenient to sample |
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| Publication Number | Publication Date |
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| CN214004669U true CN214004669U (en) | 2021-08-20 |
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| CN202022856515.5U Active CN214004669U (en) | 2020-12-02 | 2020-12-02 | Biological cell culture device convenient to sample |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116676192A (en) * | 2023-06-07 | 2023-09-01 | 上海多宁生物科技股份有限公司 | Bioreactor convenient for extracting and supplementing materials and used for CHO cell culture and method thereof |
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2020
- 2020-12-02 CN CN202022856515.5U patent/CN214004669U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116676192A (en) * | 2023-06-07 | 2023-09-01 | 上海多宁生物科技股份有限公司 | Bioreactor convenient for extracting and supplementing materials and used for CHO cell culture and method thereof |
| CN116676192B (en) * | 2023-06-07 | 2023-11-14 | 上海多宁生物科技股份有限公司 | Bioreactor convenient for extracting and supplementing materials and used for CHO cell culture and method thereof |
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