CN211595624U - Stem cell culture medium rapid cooling device - Google Patents
Stem cell culture medium rapid cooling device Download PDFInfo
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- CN211595624U CN211595624U CN201922437876.3U CN201922437876U CN211595624U CN 211595624 U CN211595624 U CN 211595624U CN 201922437876 U CN201922437876 U CN 201922437876U CN 211595624 U CN211595624 U CN 211595624U
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Abstract
The utility model relates to the technical field of biological science, in particular to a stem cell culture medium rapid cooling device, which comprises a seal box, a cooling device, a rapid cooling frame, a transmission device and a release frame, wherein the seal box comprises a box body, a placing door, an observation window, a transmission motor, a rotating wheel and a collecting box, the cooling device comprises a rapid cooling box, a cooling liquid storing box, a cooling pipe and a vacuum pump, trachea and vacuum pump output tube are received to the vacuum pump, and quick cooling frame includes sealing door, rotation seat, joint, base, sliding plate and culture medium fixed plate, and transmission includes promotion motor, driving shaft, main bevel gear, transmission bevel gear and transmission lead screw, and the release frame is including placing case, slider, limiting plate, push motor and push screw, the utility model discloses can carry out low temperature storage culture medium and also can carry out quick cooling stem cell culture medium, protection test product.
Description
Technical Field
The utility model relates to a biological science technical field especially relates to a stem cell culture medium rapid cooling device.
Background
The culture medium is the basic tool for carrying out biological cell culture, but at present, in the subpackaging process after the sterilization of the culture medium, the temperature of the stem cell culture medium is too high, so that the survival amount of stem cells is reduced, the culture of the stem cells is very unfavorable, and the traditional laboratory cooling mode can only slowly reduce the temperature, needs a large amount of time, causes great damage to the cultured stem cells, and increases the difficulty of culture.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a stem cell culture medium rapid cooling device can carry out low temperature storage culture medium and also can carry out rapid cooling stem cell culture medium, protection test product.
The purpose of the utility model is realized through the following technical scheme:
a stem cell culture medium rapid cooling device comprises a seal box, a cooling device, a rapid cooling frame, a transmission device and a release frame, wherein the cooling device is fixedly connected in the seal box;
the sealing box comprises a box body, a placing door, an observation window, a transmission motor, two rotating wheels and a collecting box, wherein the placing door is hinged on the box body;
the cooling device comprises a quick cooling box, a cooling liquid storage box, a cooling pipe, a vacuum pump air receiving pipe and a vacuum pump output pipe, wherein the quick cooling box is fixedly connected in the box body;
the rapid cooling rack comprises a sealing door, a rotating seat, a joint, a base, a sliding plate and a culture medium fixing plate, wherein the sealing door is connected with the rapid cooling box in a sliding manner;
the transmission device comprises a lifting motor, a driving shaft, a main bevel gear, transmission bevel gears and a transmission screw rod, wherein the lifting motor is fixedly connected to the box body, the driving shaft is fixedly connected to an output shaft of the lifting motor, the driving shaft is rotatably connected to the box body, the main bevel gear is fixedly connected to the left end of the driving shaft, the two transmission bevel gears are in meshing transmission with the main bevel gear, the two transmission bevel gears are fixedly connected to the transmission screw rod, and the transmission screw rod is rotatably connected to the box body;
the release frame including placing case, slider, limiting plate, pushing motor and promotion lead screw, slider fixed connection is in the rear end of placing the case, slider sliding connection is on the box, limiting plate sliding connection is in placing the incasement, the limiting plate passes through screw drive with the promotion lead screw and is connected, the rear end fixed connection of promotion lead screw is on pushing motor's output shaft, pushing motor fixed connection is in placing the incasement, it passes through screw drive with the transmission lead screw and is connected to place the case.
The utility model has the advantages that:
the utility model provides a stem cell culture medium rapid cooling device, coolant liquid storage pump and cooling tube are always in operation state, when rapid cooling is needed, the sealing door is pulled out, then the sliding plate and the culture medium fixing plate are pulled out to place the culture medium in the fixing groove on the culture medium fixing plate, the sliding plate and the culture medium fixing plate are pulled back, the sealing door is closed, the vacuum pump is started, moisture on the surface of the culture medium is rapidly evaporated and absorbed to rapidly cool down the heat by using the extracted air, when the culture medium is normally stored and observed, the placing door is opened, the placing box is lifted by the starting motor lifting motor, the culture medium is placed in the placing box, the lifting motor is restarted to descend the placing box, the pushing motor is started when the lowest end is reached, the transmission motor is simultaneously started, the limiting plate slides out, the culture medium sequentially falls on the conveying belt from back to front in the placing box, the equal distribution is on the conveyer belt, and the accessible observation window is observed, when needing to take out, and the motor is said again and the culture medium is conveyed the collecting box in, and this device not only can carry out low temperature storage culture medium and also can carry out rapid cooling stem cell culture medium.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention;
fig. 2 is a cross-sectional view of the overall structure of the present invention;
FIG. 3 is a schematic view of the overall structure of the sealing box of the present invention;
FIG. 4 is a sectional view of the seal box structure of the present invention;
FIG. 5 is a schematic view of the overall structure of the cooling device of the present invention;
FIG. 6 is a schematic view of the overall structure of the rapid cooling rack of the present invention;
fig. 7 is a schematic view of the overall structure of the transmission device of the present invention;
FIG. 8 is a schematic view of the overall structure of the release frame of the present invention;
fig. 9 is a sectional view of the structure of the release frame of the present invention.
In the figure: a seal box 1; a box body 1-1; placing a door 1-2; 1-3 of an observation window; 1-4 of a transmission motor; 1-5 of a rotating wheel; collecting boxes 1-6; a cooling device 2; 2-1 of a quick cooling box; a coolant storage pump 2-2; 2-3 of a cooling pipe; 2-4 of a vacuum pump; a vacuum pump gas collecting pipe 2-5; a vacuum pump output pipe 2-6; a rapid cooling rack 3; a sealing door 3-1; 3-2 of a rotating seat; 3-3 of a joint; 3-4 parts of a base; a sliding plate 3-5; 3-6 parts of a culture medium fixing plate; a transmission device 4; a hoisting motor 4-1; 4-2 of a driving shaft; 4-3 of a main bevel gear; 4-4 of transmission bevel gear; 4-5 of a transmission screw rod; a release frame 5; placing a box 5-1; 5-2 of a slide block; 5-3 of a limiting plate; 5-4 of a pushing motor; 5-5 of a pushing screw rod.
Detailed Description
The present invention will be described in further detail with reference to the accompanying fig. 1-9.
The first embodiment is as follows:
as shown in fig. 1-9, a stem cell culture medium rapid cooling device comprises a seal box 1, a cooling device 2, a rapid cooling frame 3, a transmission device 4 and a release frame 5, wherein the cooling device 2 is fixedly connected in the seal box 1, the rapid cooling frame 3 is slidably connected in the cooling device 2, the front end of the rapid cooling frame 3 is hinged with the seal box 1, the transmission device 4 is fixedly connected with the seal box 1, the transmission device 4 is in threaded transmission connection with the release frame 5, the release frame 5 is slidably connected in the seal box 1, and two rotating wheels 1-5 are rotatably connected on a box body 1-1;
the cooling liquid storage pump 2-2 and the cooling pipe 2-3 are always in an operating state, when rapid cooling is needed, the sealing door 3-1 is pulled out, then the sliding plate 3-5 and the culture medium fixing plate 3-6 are pulled out, the culture medium is placed in a fixing groove on the culture medium fixing plate 3-6, the sliding plate 3-5 and the culture medium fixing plate 3-6 are pulled back, the sealing door 3-1 is closed, the vacuum pump 2-4 is started, the moisture on the surface of the culture medium is rapidly evaporated and absorbed by using the extracted air to rapidly cool down, when in ordinary storage and observation, the placing door 1-2 is opened, the motor lifting motor 4-1 is started to lift the placing box 5-1, the culture medium is placed into the placing box 5-1, the lifting motor 4-1 is started again to make the placing box 5-1 fall, when the culture medium falls to the lowest end, the pushing motor 5-4 is started, the transmission motor 1-4 is started at the same time, the limiting plate 5-3 slides out, the culture medium sequentially falls on the conveying belt from back to front in the placing box 5-1 and is uniformly distributed on the conveying belt, the culture medium can be observed through the observation window 1-3, and when the culture medium needs to be taken out, the culture medium is conveyed into the collection box 1-6 by the transmission motor 1-4.
The second embodiment is as follows:
as shown in fig. 1-9, the seal box 1 comprises a box body 1-1, a placing door 1-2, an observation window 1-3, a transmission motor 1-4, two rotating wheels 1-5 and a collecting box 1-6, wherein the placing door 1-2 is hinged on the box body 1-1, the observation window 1-3 is fixedly connected with the front end of the box body 1-1, the transmission motor 1-4 is fixedly connected on the box body 1-1, the rotating wheels 1-5 are provided, the rotating wheels 1-5 positioned at the rear side are fixedly connected on an output shaft of the transmission motor 1-4, the two rotating wheels 1-5 are in transmission connection through a transmission belt, and the collecting box 1-6 is fixedly connected in the box body 1-1;
the box body is made of heat insulation materials, the placing door 1-2 is hinged on the box body 1-1 and sealed, the transmission motor 1-4 is started, and the two rotating wheels 1-5 rotate the conveyor belt to start to operate.
The third concrete implementation mode:
as shown in fig. 1-9, the cooling device 2 comprises a quick cooling box 2-1, a cooling liquid storage pump 2-2 and a cooling pipe 2-3, the cooling system comprises a vacuum pump 2-4, a vacuum pump air receiving pipe 2-5 and a vacuum pump output pipe 2-6, wherein a quick cooling box 2-1 is fixedly connected in a box body 1-1, a cooling pipe 2-3 is fixedly connected on the quick cooling box 2-1, a cooling liquid storage pump 2-2 is fixedly connected and communicated with the cooling pipe 2-3, the cooling liquid storage pump 2-2 is fixedly connected on the box body 1-1, the vacuum pump air receiving pipe 2-5 is fixedly connected and communicated with the quick cooling box 2-1, the vacuum pump air receiving pipe 2-5 and the vacuum pump output pipe 2-6 are both fixedly connected on the vacuum pump 2-4, and the vacuum pump 2-4 is fixedly connected on the box body 1-1;
and starting a cooling liquid storage pump 2-2 to enable cooling liquid to flow in the cooling pipe 2-3 to reduce the temperature in the whole device, wherein the vacuum pump 2-4 adopts a vacuum pump model XD-0040F, and can also be selected in the market according to actual requirements.
The fourth concrete implementation mode:
as shown in fig. 1-9, the rapid cooling rack 3 comprises a sealing door 3-1, a rotating seat 3-2 and a joint 3-3, the culture medium box comprises a base 3-4, a sliding plate 3-5 and a culture medium fixing plate 3-6, wherein a sealing door 3-1 is in sliding connection with a quick cooling box 2-1, the lower end of the sealing door 3-1 is hinged to a box body 1-1, a rotating seat 3-2 is fixedly connected to the sealing door 3-1, the rotating seat 3-2 is rotatably connected with a joint 3-3, the base 3-4 is fixedly connected to the joint 3-3, the base 3-4 is in sliding connection with the inside of the quick cooling box 2-1, the sliding plate 3-5 is in sliding connection with the base 3-4, and the upper end of the sliding plate 3-5 is fixedly connected to the lower end of the culture medium fixing plate 3-;
the sealing door 3-1 is pulled out, the base 3-4 and the sliding plate 3-5 are pulled out, the culture medium is placed on the base 3-4, the base 3-4 and the sliding plate 3-5 are pushed back, the sealing door 3-1 is closed, the vacuum pump 2-4 is started, air in the quick cooling box 2-1 is pumped out, moisture on the surface of the culture medium is quickly evaporated to take away a large amount of heat, and the temperature of the culture medium is quickly reduced.
The fifth concrete implementation mode:
as shown in fig. 1-9, the transmission device 4 includes a lifting motor 4-1, a driving shaft 4-2, a main bevel gear 4-3, a transmission bevel gear 4-4 and a transmission lead screw 4-5, the lifting motor 4-1 is fixedly connected to the box 1-1, the driving shaft 4-2 is fixedly connected to an output shaft of the lifting motor 4-1, the driving shaft 4-2 is rotatably connected to the box 1-1, the main bevel gear 4-3 is fixedly connected to the left end of the driving shaft 4-2, the transmission bevel gears 4-4 are engaged with the main bevel gear 4-3 for transmission, the transmission bevel gears 4-4 are fixedly connected to the transmission lead screw 4-5, and the transmission lead screw 4-5 is rotatably connected to the box 1-1;
when low-temperature storage is needed, the lifting motor 4-1 is started, the driving shaft 4-2 and the main bevel gear 4-3 rotate to drive the two transmission bevel gears 4-4 engaged with the main bevel gear 4-3 to rotate, so that the transmission screw rod 4-5 rotates.
The sixth specific implementation mode:
as shown in fig. 1-9, the release frame 5 comprises a placing box 5-1, a sliding block 5-2, a limiting plate 5-3, a pushing motor 5-4 and a pushing screw rod 5-5, wherein the sliding block 5-2 is fixedly connected to the rear end of the placing box 5-1, the sliding block 5-2 is slidably connected to a box body 1-1, the limiting plate 5-3 is slidably connected to the placing box 5-1, the limiting plate 5-3 is in threaded transmission connection with the pushing screw rod 5-5, the rear end of the pushing screw rod 5-5 is fixedly connected to an output shaft of the pushing motor 5-4, the pushing motor 5-4 is fixedly connected to the placing box 5-1, and the placing box 5-1 is in threaded transmission connection with the driving screw rod 4-5;
the transmission screw rod 4-5 rotates to enable the placing box 5-1 to move up and down, and meanwhile, the pushing motor 5-5 is started to push the screw rod 5-5 to rotate to push the limiting plate 5-3 out, so that the culture medium can fall down.
The utility model relates to a stem cell culture medium rapid cooling device, its theory of use is: the cooling liquid storage pump 2-2 and the cooling pipe 2-3 are always in an operating state, when rapid cooling is needed, the sealing door 3-1 is pulled out, then the sliding plate 3-5 and the culture medium fixing plate 3-6 are pulled out, the culture medium is placed in a fixing groove on the culture medium fixing plate 3-6, the sliding plate 3-5 and the culture medium fixing plate 3-6 are pulled back, the sealing door 3-1 is closed, the vacuum pump 2-4 is started, the moisture on the surface of the culture medium is rapidly evaporated and absorbed by using the extracted air to rapidly cool down, when in ordinary storage and observation, the placing door 1-2 is opened, the motor lifting motor 4-1 is started to lift the placing box 5-1, the culture medium is placed into the placing box 5-1, the lifting motor 4-1 is started again to make the placing box 5-1 fall, starting a pushing motor 5-4 when the culture medium falls to the lowest end, starting a transmission motor 1-4 at the same time, sliding out a limiting plate 5-3, sequentially dropping the culture medium on a conveyor belt from back to front in a placing box 5-1, uniformly distributing the culture medium on the conveyor belt, observing the culture medium through an observation window 1-3, when the culture medium needs to be taken out, conveying the culture medium into a collecting box 1-6 by the transmission motor 1-4, adopting a heat insulation material for the box body, hinging and sealing the placing door 1-2 on the box body 1-1, starting the transmission motor 1-4, starting the conveyor belt by two rotating wheels 1-5, starting a cooling liquid storage pump 2-2 to enable the cooling liquid to flow in a cooling pipe 2-3, reducing the temperature in the whole device, using an XD-0040F type vacuum pump for the vacuum pump 2-4, and selecting the vacuum pump in the market according to the, pulling out the sealing door 3-1, pulling out the base 3-4 and the sliding plate 3-5, placing the culture medium on the base 3-4, pushing back the base 3-4 and the sliding plate 3-5, closing the sealing door 3-1, starting the vacuum pump 2-4, pumping out the air in the quick cooling box 2-1, quickly evaporating the moisture on the surface of the culture medium to take away a large amount of heat, quickly reducing the temperature of the culture medium, opening the placing door 1-2 to place the culture medium in the placing box 5-1 when low-temperature storage is needed, starting the lifting motor 4-1, rotating the driving shaft 4-2 and the main bevel gear 4-3 to drive the two transmission bevel gears 4-4 engaged with the main bevel gear 4-3 to rotate, rotating the transmission screw rod 4-5 to enable the placing box 5-1 to move up and down, meanwhile, the pushing motor 5-5 is started to push the screw rod 5-5 to rotate so as to push the limiting plate 5-3 out, and the culture medium can fall down.
Of course, the above description is not intended to limit the present invention, and the present invention is not limited to the above examples, and the changes, modifications, additions or replacements made by those skilled in the art within the scope of the present invention also belong to the protection scope of the present invention.
Claims (6)
1. The utility model provides a quick cooling device of stem cell culture medium, includes seal box (1), cooling device (2), quick cooling frame (3), transmission (4) and release frame (5), its characterized in that: cooling device (2) fixed connection is in seal box (1), and quick cooling frame (3) sliding connection is in cooling device (2), and the front end of quick cooling frame (3) is articulated with seal box (1), and transmission (4) and seal box (1) fixed connection, transmission (4) and release frame (5) are connected through screw thread transmission, and release frame (5) sliding connection is in seal box (1).
2. The rapid cooling device for stem cell culture medium according to claim 1, characterized in that: the sealing box (1) comprises a box body (1-1), a placing door (1-2), an observation window (1-3), a transmission motor (1-4), a rotating wheel (1-5) and a collecting box (1-6), the placement door (1-2) is hinged to the box body (1-1), the observation window (1-3) is fixedly connected to the front end of the box body (1-1), the transmission motor (1-4) is fixedly connected to the box body (1-1), two rotating wheels (1-5) are arranged, the rotating wheel (1-5) located on the rear side is fixedly connected to an output shaft of the transmission motor (1-4), the two rotating wheels (1-5) are in transmission connection through a transmission belt, the collection box (1-6) is fixedly connected into the box body (1-1), and the two rotating wheels (1-5) are rotatably connected to the box body (1-1).
3. The rapid cooling device for stem cell culture medium according to claim 2, characterized in that: the cooling device (2) comprises a quick cooling box (2-1), a cooling liquid storage pump (2-2), a cooling pipe (2-3), a vacuum pump (2-4), a vacuum pump air receiving pipe (2-5) and a vacuum pump output pipe (2-6), wherein the quick cooling box (2-1) is fixedly connected in the box body (1-1), the cooling pipe (2-3) is fixedly connected on the quick cooling box (2-1), the cooling liquid storage pump (2-2) is fixedly connected and communicated with the cooling pipe (2-3), the cooling liquid storage pump (2-2) is fixedly connected on the box body (1-1), the vacuum pump air receiving pipe (2-5) is fixedly connected and communicated on the quick cooling box (2-1), the vacuum pump air receiving pipe (2-5) and the vacuum pump output pipe (2-6) are both fixedly connected on the vacuum pump (2-4), the vacuum pump (2-4) is fixedly connected to the box body (1-1).
4. The rapid cooling device for stem cell culture medium according to claim 3, wherein: the rapid cooling rack (3) comprises a sealing door (3-1), a rotating seat (3-2), a joint (3-3), a base (3-4), a sliding plate (3-5) and a culture medium fixing plate (3-6), wherein the sealing door (3-1) is in sliding connection with the rapid cooling box (2-1), the sealing door (3-1) is hinged on the box body (1-1), the rotating seat (3-2) is fixedly connected on the sealing door (3-1), the rotating seat (3-2) is in rotating connection with the joint (3-3), the base (3-4) is fixedly connected on the joint (3-3), the base (3-4) is in sliding connection in the rapid cooling box (2-1), the sliding plate (3-5) is in sliding connection on the base (3-4), the upper end of the sliding plate (3-5) is fixedly connected with the lower end of the culture medium fixing plate (3-6).
5. The device for rapidly cooling stem cell culture media according to claim 4, wherein: the transmission device (4) comprises a lifting motor (4-1), a driving shaft (4-2), a main bevel gear (4-3), a transmission bevel gear (4-4) and a transmission screw rod (4-5), the lifting motor (4-1) is fixedly connected to the box body (1-1), the driving shaft (4-2) is fixedly connected to an output shaft of the lifting motor (4-1), the driving shaft (4-2) is rotatably connected to the box body (1-1), the main bevel gear (4-3) is fixedly connected to the left end of the driving shaft (4-2), the transmission bevel gear (4-4) is in meshing transmission with the main bevel gear (4-3), the transmission bevel gear (4-4) is fixedly connected to the transmission screw rod (4-5), and the transmission screw rod (4-5) is rotatably connected to the box body (1-1).
6. The rapid cooling device for stem cell culture medium according to claim 5, wherein: the release frame (5) comprises a placing box (5-1) and a sliding block (5-2), the device comprises a limiting plate (5-3), a pushing motor (5-4) and a pushing screw rod (5-5), wherein a sliding block (5-2) is fixedly connected to the rear end of a placing box (5-1), the sliding block (5-2) is slidably connected to a box body (1-1), the limiting plate (5-3) is slidably connected into the placing box (5-1), the limiting plate (5-3) is in threaded transmission connection with the pushing screw rod (5-5), the rear end of the pushing screw rod (5-5) is fixedly connected to an output shaft of the pushing motor (5-4), the pushing motor (5-4) is fixedly connected into the placing box (5-1), and the placing box (5-1) is in threaded transmission connection with the transmission screw rod (4-5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922437876.3U CN211595624U (en) | 2019-12-30 | 2019-12-30 | Stem cell culture medium rapid cooling device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922437876.3U CN211595624U (en) | 2019-12-30 | 2019-12-30 | Stem cell culture medium rapid cooling device |
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| CN211595624U true CN211595624U (en) | 2020-09-29 |
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| CN201922437876.3U Active CN211595624U (en) | 2019-12-30 | 2019-12-30 | Stem cell culture medium rapid cooling device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114017966A (en) * | 2021-11-02 | 2022-02-08 | 江苏蒙彼利生物科技有限公司 | Refrigeration device and refrigeration method for culturing non-blood stem cells |
-
2019
- 2019-12-30 CN CN201922437876.3U patent/CN211595624U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114017966A (en) * | 2021-11-02 | 2022-02-08 | 江苏蒙彼利生物科技有限公司 | Refrigeration device and refrigeration method for culturing non-blood stem cells |
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