CN114017966A - Refrigeration device and refrigeration method for culturing non-blood stem cells - Google Patents
Refrigeration device and refrigeration method for culturing non-blood stem cells Download PDFInfo
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- CN114017966A CN114017966A CN202111287670.2A CN202111287670A CN114017966A CN 114017966 A CN114017966 A CN 114017966A CN 202111287670 A CN202111287670 A CN 202111287670A CN 114017966 A CN114017966 A CN 114017966A
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- placing
- box
- hole
- threaded rod
- test tube
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
- F25D11/00—Self-contained movable devices, e.g. domestic refrigerators
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
- F25D23/00—General constructional features
- F25D23/02—Doors; Covers
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
- F25D25/00—Charging, supporting, and discharging the articles to be cooled
- F25D25/02—Charging, supporting, and discharging the articles to be cooled by shelves
- F25D25/024—Slidable shelves
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
- F25D3/00—Devices using other cold materials; Devices using cold-storage bodies
- F25D3/12—Devices using other cold materials; Devices using cold-storage bodies using solidified gases, e.g. carbon-dioxide snow
Abstract
The invention discloses a refrigerating device and a refrigerating method for culturing non-blood stem cells. The invention has the beneficial effects that: the test tube placing device is provided with the fixing assembly, the placing plate can be driven to rotate through the second transmission rod, dry ice can be uniformly scattered in the placing groove, direct contact between the dry ice and a test tube is reduced, the dry ice is convenient to add, the one-way threaded rod is arranged, the motor and the drive gear are mutually meshed, the one-way threaded rod can be driven to rotate, the test tube can be lifted through limiting of the bottom plate and the top plate, a worker can take out and place the test tube conveniently, the sealing cover is provided with the taking-out hole, and temperature loss of the worker during taking and placing the test tube can be reduced.
Description
Technical Field
The invention relates to a refrigerating device, in particular to a refrigerating device and a refrigerating method for culturing non-blood stem cells, and belongs to the technical field of culturing non-blood stem cells.
Background
Stem cells are a class of cells with unlimited or immortal self-renewal capacity, capable of producing at least one type and highly differentiated progeny cells, and most biologists and physicians now believe that stem cells are cells from embryonic, fetal or adult sources, with unlimited self-renewal and proliferative differentiation capacity under certain conditions, capable of producing daughter cells with identical phenotype and genotype as well as self, and capable of producing specialized cells that make up body tissues, organs, and also capable of differentiating into progenitor cells.
When cultivateing stem cell, stem cell culture medium need be placed and store in the low temperature usually, current refrigerating plant uses liquid nitrogen or dry ice refrigeration mostly, the dry ice is piled up in the refrigerating plant bottom mostly placing the in-process, inconvenient interpolation during later stage interpolation, place in-process dry ice easy and stem cell culture medium laminating, it is impaired to cause stem cell culture medium to preserve, and the staff need bow when taking stem cell culture medium and stretch into refrigerating plant with the hand, make a round trip to get the longer refrigerant loss that increases among the refrigerating plant of in-process time of putting easily.
Disclosure of Invention
In order to solve the above problems, an object of the present invention is to provide a refrigerator for culturing non-hematopoietic stem cells and a refrigerating method.
The present invention is to achieve the above object by the following means, and a refrigerator for culturing non-hematopoietic stem cells and a refrigerating method, comprising:
the ice storage box comprises a storage box body, wherein supporting legs are arranged at four corners of the bottom of the storage box body, a storage cover is arranged at the top of the storage box body, a first handle is arranged at the top of the storage cover, a fixed box is arranged on the outer side of the storage box body, a storage groove is formed in the top of the fixed box body, and a dry ice box is arranged in the storage groove;
the mounting box is mounted at the bottom of the placing box through bolts, a motor is mounted inside the mounting box, a refrigerating cylinder is mounted at the bottom inside the placing box, and the placing box is connected to the inner wall of the refrigerating cylinder in a sliding mode;
the moving assembly is arranged inside the placing box and comprises a bidirectional threaded rod, the top of the placing box is provided with an accommodating groove, the inner wall of the accommodating groove is connected with a bottom plate in a sliding mode, two ends of the top of the bottom plate are provided with connecting rods, and the top of each connecting rod is provided with a top plate;
the fixed subassembly, fixed subassembly sets up the inside at fixed case, fixed subassembly is including articulated piece, the outside of articulated piece articulates there is the second transfer line, the spout has all been seted up at the inside both ends of fixed case, the inside slidable mounting of spout has the slider, the installation piece is installed to the one end of slider.
Preferably, the one side of placing case and cold-stored section of thick bamboo and laminating each other has seted up the interlude hole, the output of motor extends to the inside of cold-stored section of thick bamboo through the interlude hole, first gear is installed to the output of motor, the inside one end bearing of cold-stored section of thick bamboo rotates installs one-way threaded rod, the second gear is installed in the outside of one-way threaded rod bottom.
Preferably, the inside other end of cold-stored section of thick bamboo is installed the gag lever post, the rack is installed at the positive top of gag lever post, the one end of placing the box is seted up threaded hole, the inside screw thread of screw hole rotates and is connected with one-way threaded rod, the other end of placing the box is installed the guiding hole, the inside slip interlude of guiding hole is connected with the gag lever post.
Preferably, place the bottom of incasement wall and install the slide, the top slidable mounting of slide has the mounting plate, the connecting block is installed in the outside of mounting plate, the pole setting is installed at the top of connecting block, the central point in the pole setting outside puts and installs the stopper.
Preferably, the inner wall of placing the case has seted up the guide way, the inside sliding connection of guide way has the stopper, the top of fixed case is provided with sealed lid, the second handle is all installed at the both ends at sealed lid top, the fixed orifices has been seted up to the one end at sealed lid top, the inside cover of fixed orifices is equipped with the pole setting.
Preferably, the two ends of the bidirectional threaded rod are provided with moving blocks in a reverse threaded manner, and the front surfaces of the moving blocks are hinged with first transmission rods.
Preferably, the locating piece is all installed at the both ends of bottom plate bottom, the other end and the locating piece of first transfer line are articulated mutually, place the inside of box and seted up the linkage hole, the inside bearing in linkage hole rotates and is connected with two-way threaded rod, the third gear is installed in the outside that two-way threaded rod extends to linkage hole one end, the spring is installed at the top of slider, the top of spring and the inner wall fixed connection of fixed case.
Preferably, the bottom of second transfer line one end articulates there is the pull rod, the removal hole has all been seted up at the both ends of fixed case, the inside slip interlude in removal hole is connected with the pull rod, the guide block is installed in the front of installation piece, the restriction hole has been seted up in the front of second transfer line, the inside sliding connection in restriction hole has the guide block.
Preferably, the top of the bottom plate is provided with a placing groove, the top plate is provided with a placing hole, the placing groove is located at the bottom of the placing hole, the inside of the placing hole is sleeved with the test tube, and the placing groove corresponds to the same size as the test tube.
The refrigeration method for culturing the non-blood stem cells comprises the following steps;
the first step is as follows: the dry ice box is placed in a placing groove of the fixed box and pressed downwards, the dry ice box can be fixed through the fixing assembly, the dry ice can enter the placing groove of the placing plate through the discharge hole, and the dry ice sublimes to absorb heat so as to refrigerate the interior of the placing box;
the second step is that: when the test tube is taken, the motor is started, the motor drives the first gear to rotate, the first gear drives the second gear to rotate, the second gear drives the one-way threaded rod to rotate, and the placing box is driven to ascend through the one-way threaded rod;
the third step: place the box at the in-process that rises, the third gear receives blockking of rack to rotate, and the third gear drives two-way threaded rod and rotates, and two-way threaded rod passes through movable block, first transfer line and locating piece and drives the test tube and rise, and the test tube rises to the top of sealed lid, and the staff takes the test tube.
The invention has the beneficial effects that: the dry ice box is provided with a fixing component, one end of a second transmission rod is pressed, the second transmission rod can rotate, the spring can be compressed through the second transmission rod, the resilience of the spring can enable one end of the second transmission rod to fix the dry ice box, dry ice can fall into a placing groove of a placing plate through a discharge hole, the placing plate can be driven to rotate through a rotating vertical rod, the dry ice can be uniformly scattered in the placing groove, and after the dry ice in the placing groove is used up, the vertical rod is rotated again, so that the dry ice can be rapidly added.
The test tube placing device is provided with the one-way threaded rod, the one-way threaded rod can be driven to rotate by the mutual meshing of the motor and the driving gear, the placing box can vertically move up and down by the one-way threaded rod, the rotation of the two-way threaded rod can be realized by the matching use of the rack and the gear in the upward vertical movement process of the placing box, the ascending of the bottom plate can be realized by the matching use among the moving block, the transmission rod and the positioning block, the ascending of a test tube can be realized by the limiting of the bottom plate and the top plate, and the test tube can be conveniently taken out and placed by a worker.
According to the test tube box, after the dry ice is filled, the isolation of the dry ice in the box can be increased through the sealing cover, the taking hole is formed in the sealing cover in the process of taking out and placing the test tube, the temperature loss of workers when the workers take and place the test tube can be reduced, and the placing cover and the sealing cover can be taken conveniently by the workers through the placing cover and the handle arranged at the top of the sealing cover.
Drawings
FIG. 1 is a perspective view of the overall structure of the present invention;
FIG. 2 is a schematic view of the overall internal structure of the present invention;
FIG. 3 is a side view of the internal structure of the fixing box of the present invention;
FIG. 4 is a perspective view of the top structure of the sealing cap of the present invention;
FIG. 5 is a perspective view of the bottom structure of the sealing cap of the present invention;
FIG. 6 is a perspective view of the refrigeration cartridge of the present invention;
FIG. 7 is a schematic view of the internal structure of the storage case of the present invention;
FIG. 8 is an enlarged view taken at A of FIG. 2 in accordance with the present invention;
FIG. 9 is an enlarged view of the invention at B in FIG. 3.
In the figure: 1. placing a box; 2. supporting legs; 3. placing a cover; 301. a first handle; 4. a fixed box; 5. a dry ice box; 6. mounting a box; 7. a motor; 701. a first gear; 8. placing a plate; 9. connecting blocks; 901. erecting a rod; 902. a limiting block; 10. a cold storage drum; 11. a sealing cover; 1101. a second handle; 12. a one-way threaded rod; 1201. a second gear; 13. placing the box; 14. a limiting rod; 1401. a rack; 15. a bidirectional threaded rod; 1501. a moving block; 1502. a third gear; 16. a first drive lever; 17. a base plate; 1701. positioning blocks; 18. a connecting rod; 19. a top plate; 20. a test tube; 21. a hinged block; 22. a second transmission rod; 23. a pull rod; 24. a spring; 25. a slider; 26. mounting blocks; 2601. and a guide block.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-9, a refrigerating apparatus and a refrigerating method for culturing non-stem cells includes:
the placing box comprises a placing box 1, supporting legs 2 are mounted at four corners of the bottom of the placing box 1, the four supporting legs 2 can play a role of fixedly supporting the placing box 1, a placing cover 3 is arranged at the top of the placing box 1, a through hole is formed in the top of the placing box 1, an arc-shaped positioning groove is formed in the top of the outer side of the placing box 1, the positioning groove corresponds to and is the same as the placing cover 3 in size, the placing cover 3 can play a role of hermetically placing the box 1, a first handle 301 is mounted at the top of the placing cover 3, and the first handle 301 is convenient for workers to take the placing cover 3;
the fixed box 4 is installed on the outer side of the placing box 1, the fixed box 4 is U-shaped, a placing groove is formed in the top of the fixed box 4, a dry ice box 5 is arranged inside the placing groove, a discharging hole is formed in one side, close to the placing box 1, of the dry ice box 5, an adding hole is formed in one side of the dry ice box 5, dry ice can be added into the dry ice box 5 through the adding hole, the dry ice is solid and can sublimate at normal temperature, heat can be absorbed during sublimation, and therefore the temperature inside the placing box 1 is reduced;
the bottom of the inner wall of the placing box 1 is provided with a sliding plate, the top of the sliding plate is provided with a placing plate 8 in a sliding mode, the placing plate 8 is in a ring shape, the top of the placing plate 8 is provided with a placing groove, the placing groove is level to the position of a discharge hole of the placing box 1, dry ice in the dry ice box 5 can enter the placing groove through the discharge hole, the outer side of the placing plate 8 is provided with a connecting block 9, the top of the connecting block 9 is provided with a vertical rod 901, the central position of the outer side of the vertical rod 901 is provided with a limiting block 902, and the position of the vertical rod 901 can be limited through the limiting block 902;
a through hole is formed in one surface, which is attached to the refrigerating cylinder 10, of the placing box 1, the output end of the motor 7 extends into the refrigerating cylinder 10 through the through hole, a first gear 701 is installed at the output end of the motor 7, a one-way threaded rod 12 is installed in the refrigerating cylinder 10 in a rotating mode through a bearing at one end of the inside of the one-way threaded rod 12, a second gear 1201 is installed on the outer side of the bottom of the one-way threaded rod 12, the first gear 701 and the second gear 1201 are meshed with each other, and the first gear 701 rotates to drive the second gear 1201 to rotate synchronously;
the other end of the interior of the refrigeration cylinder 10 is provided with a limiting rod 14, the outer side of the refrigeration cylinder 10 is provided with a plurality of air holes, the top of the front face of the limiting rod 14 is provided with a rack 1401, a third gear 1502 is mutually meshed with the rack 1401, one end of the placing box 13 is provided with a threaded hole, the internal thread of the threaded hole is rotatably connected with a one-way threaded rod 12, the other end of the placing box 13 is provided with a guide hole, the interior of the guide hole is slidably inserted and connected with the limiting rod 14, and the placing box 13 can be limited by the limiting rod 14;
the inner wall of the placing box 1 is provided with a guide groove, the inside of the guide groove is connected with a limiting block 902 in a sliding mode, the top of the fixing box 4 is provided with a sealing cover 11, two ends of the top of the sealing cover 11 are both provided with a second handle 1101, the second handle 1101 is convenient for workers to take the sealing cover 11, one end of the top of the sealing cover 11 is provided with a fixing hole, a vertical rod 901 is sleeved inside the fixing hole, and the vertical rod 901 can be limited through the fixing hole;
the refrigerator comprises a mounting box 6, wherein the mounting box 6 is mounted at the bottom of a placing box 1 through bolts, a motor 7 is mounted inside the mounting box 6, a refrigerating cylinder 10 is mounted at the bottom inside the placing box 1, a placing box 13 is connected to the inner wall of the refrigerating cylinder 10 in a sliding mode, and a plurality of air holes are formed in the bottom of the placing box 13;
the moving assembly is arranged inside the placing box 13 and comprises a bidirectional threaded rod 15, the top of the placing box 13 is provided with a containing groove, the inner wall of the containing groove is connected with a bottom plate 17 in a sliding mode, two ends of the top of the bottom plate 17 are provided with connecting rods 18, the top of each connecting rod 18 is provided with a top plate 19, the outer sides of two ends of the bidirectional threaded rod 15 are provided with threads with opposite turning directions, and the bidirectional threaded rod 15 rotates to enable the moving blocks 1501 at two ends to be driven to be close to each other;
the top of the bottom plate 17 is provided with a placing groove, the placing groove is arc-shaped, the arc-shaped placing groove can be more attached to the test tube 20, the stability of the test tube 20 is improved, a placing hole is formed in the top plate 19, the placing groove is located at the bottom of the placing hole, the test tube 20 is sleeved in the placing hole, and the placing groove is correspondingly the same as the test tube 20 in size;
positioning blocks 1701 are arranged at two ends of the bottom plate 17, the other end of the first transmission rod 16 is hinged with the positioning blocks 1701, a linkage hole is formed in the placing box 13, a bidirectional threaded rod 15 is rotatably connected to an inner bearing of the linkage hole, a third gear 1502 is arranged on the outer side of one end, extending to the linkage hole, of the bidirectional threaded rod 15, a spring 24 is arranged at the top of the sliding block 25, the self elasticity of the spring 24 can realize the up-and-down movement of the sliding block 25 under the condition of external force, and the top of the spring 24 is fixedly connected with the inner wall of the fixed box 4;
the moving blocks 1501 are rotatably mounted on the outer sides of the two ends of the bidirectional threaded rod 15 in reverse threads, the front faces of the moving blocks 1501 are hinged to the first transmission rod 16, the two moving blocks 1501 are driven to approach or separate from each other when the bidirectional threaded rod 15 rotates, and the moving blocks 1501 can drive one end of the bottom of the first transmission rod 16 to approach or separate from each other synchronously, so that the top of the first transmission rod 16 is lifted;
the fixing assembly is arranged inside the fixing box 4 and comprises a hinge block 21, a second transmission rod 22 is hinged to the outer side of the hinge block 21, sliding grooves are formed in two ends of the inside of the fixing box 4, a sliding block 25 is slidably mounted inside the sliding grooves, and a mounting block 26 is mounted at one end of the sliding block 25;
the bottom of second transfer line 22 one end articulates there is pull rod 23, and the removal hole has all been seted up at the both ends of fixed case 4, and the inside slip interlude in removal hole is connected with pull rod 23, and guide block 2601 is installed in the front of installation piece 26, and the restriction hole has been seted up in the front of second transfer line 22, and the inside sliding connection in restriction hole has guide block 2601.
The refrigeration method for culturing the non-blood stem cells comprises the following steps;
the first step is as follows: the dry ice box 5 is placed in a placing groove of the fixed box 4 and pressed downwards, the dry ice box 5 can be fixed through the fixing assembly, dry ice can enter the placing groove of the placing plate 8 through the discharging hole, and the dry ice sublimes and absorbs heat to refrigerate the interior of the placing box 1;
the second step is that: when the test tube 20 is taken, the motor 7 is started, the motor 7 drives the first gear 701 to rotate, the first gear 701 drives the second gear 1201 to rotate, the second gear 1201 drives the one-way threaded rod 12 to rotate, and the placing box 13 is driven to ascend through the one-way threaded rod 12;
the third step: placing box 13 in the in-process that rises, third gear 1502 receives the stopping of rack 1401 to rotate, and third gear 1502 drives two-way threaded rod 15 and rotates, and two-way threaded rod 15 passes through movable block 1501, first transfer lever 16 and locating piece 1701 and drives the test tube 20 and rise, and test tube 20 rises to the top of sealed lid 11, and the staff takes test tube 20.
The invention when in use refers to figures 1 to 9;
the first step of operation is implemented, when dry ice in the dry ice box 5 needs to be added, a worker pulls the pull rod 23 towards the opposite side, the pull rod 23 drives one end of the bottom of the second transmission rod 22 to be away from each other, the second transmission rod 22 is not in contact with the dry ice box 5 at the moment, the worker moves the dry ice box 5 upwards to take out the dry ice box 5, and the dry ice is added into the dry ice box 5 through an adding hole in one end of the dry ice box 5;
implementing the second step of operation, after the dry ice is added, placing the dry ice box 5 in the placing groove of the fixed box 4, pressing the dry ice box 5 downwards to enable two sides of the dry ice box 5 to be in contact with the second transmission rod 22, enabling one end of the bottom of the second transmission rod 22 to rotate downwards under pressure, driving the mounting block 26 to descend in the rotation process of the second transmission rod 22, and stretching the spring 24 in the descending process of the mounting block 26;
when the second transmission rod 22 rotates to a certain angle, one end of the bottom of the second transmission rod 22 is clamped into clamping grooves at two sides of the dry ice box 5 to be fixed, at the moment, the spring 24 is compressed through the resilience of the spring 24, the spring 24 drives the slider 25 to ascend, the slider 25 drives the mounting block 26 to ascend synchronously, the mounting block 26 drives the guide block 2601 to ascend, the guide block 2601 slides in a limiting hole of the second transmission rod 22 and drives the second transmission rod 22 to rotate, one end of the bottom of the second transmission rod 22 is close to the dry ice box 5, and the dry ice box 5 is fixed through the second transmission rod 22;
implementing the third step of operation, the dry ice in the dry ice box 5 enters the placing groove of the placing plate 8 through the dry ice box 5 and the discharging hole of the placing box 1, the placing cover 3 is taken down through the first handle 301, the sealing cover 11 is taken down through the second handle 1101, at this time, the upright stanchion 901 is not limited by the sealing cover 11, the worker rotates the upright stanchion 901, the upright stanchion 901 drives the placing plate 8 to rotate through the connecting block 9, so that the dry ice is uniformly scattered in the placing groove in the process of entering the placing plate 8, the sealing cover 11 is placed on the top of the refrigerating cylinder 10 through the second handle 1101, the placing cover 3 is placed on the top of the placing box 1 through the first handle 301, and the dry ice sublimes and absorbs heat to refrigerate the inside of the placing box 1;
implementing the fourth step of operation, when the test tube 20 needs to be taken out, the worker turns on the motor 7 through an external power supply, the motor 7 drives the first gear 701 to rotate, the first gear 701 drives the second gear 1201 to rotate, the second gear 1201 drives the one-way threaded rod 12 to rotate, and the one-way threaded rod 12 drives the placing box 13 to ascend;
when the placing box 13 is lifted to a certain position, the third gear 1502 is in contact with the rack 1401, the third gear 1502 is blocked by teeth of the rack 1401 to rotate, the third gear 1502 drives the bidirectional threaded rod 15 to rotate, the bidirectional threaded rod 15 drives the two moving blocks 1501 to approach each other, and the moving blocks 1501 drive the first transmission rod 16 to rotate;
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (10)
1. A refrigerator for culturing stem cells-free cells, comprising:
the refrigerator comprises a placing box (1), supporting legs (2) are mounted at four corners of the bottom of the placing box (1), a placing cover (3) is arranged at the top of the placing box (1), a first handle (301) is mounted at the top of the placing cover (3), a fixed box (4) is mounted on the outer side of the placing box (1), a placing groove is formed in the top of the fixed box (4), and a dry ice box (5) is arranged inside the placing groove;
the refrigerator comprises a mounting box (6), wherein the mounting box (6) is mounted at the bottom of a placing box (1) through bolts, a motor (7) is mounted inside the mounting box (6), a refrigerating cylinder (10) is mounted at the bottom inside the placing box (1), and a placing box (13) is connected to the inner wall of the refrigerating cylinder (10) in a sliding mode;
the moving assembly is arranged inside the placing box (13) and comprises a bidirectional threaded rod (15), the top of the placing box (13) is provided with an accommodating groove, the inner wall of the accommodating groove is connected with a bottom plate (17) in a sliding mode, two ends of the top of the bottom plate (17) are provided with connecting rods (18), and the top of each connecting rod (18) is provided with a top plate (19);
fixed subassembly, fixed subassembly sets up the inside at fixed case (4), fixed subassembly is including articulated piece (21), the outside of articulated piece (21) articulates there is second transfer line (22), the spout has all been seted up at the inside both ends of fixed case (4), the inside slidable mounting of spout has slider (25), installation piece (26) are installed to the one end of slider (25).
2. A refrigerator according to claim 1 for culturing hematopoietic stem cells, characterized in that: place the one side that case (1) and cold-stored section of thick bamboo (10) laminated each other and seted up the interlude hole, the output of motor (7) extends to the inside of cold-stored section of thick bamboo (10) through the interlude hole, first gear (701) are installed to the output of motor (7), the inside one end bearing of cold-stored section of thick bamboo (10) rotates and installs one-way threaded rod (12), second gear (1201) are installed in the outside of one-way threaded rod (12) bottom.
3. A refrigerator according to claim 1 for culturing hematopoietic stem cells, characterized in that: a limiting rod (14) is installed at the other end of the inside of the refrigerating cylinder (10), a rack (1401) is installed at the top of the front face of the limiting rod (14), a threaded hole is formed in one end of the placing box (13), a one-way threaded rod (12) is connected to the inner thread of the threaded hole in a rotating mode, a guide hole is installed at the other end of the placing box (13), and the limiting rod (14) is connected to the inner portion of the guide hole in a sliding and penetrating mode.
4. A refrigerator according to claim 1 for culturing hematopoietic stem cells, characterized in that: the bottom of placing the inner wall of the box (1) is provided with a sliding plate, the top of the sliding plate is provided with a placing plate (8) in a sliding manner, the outer side of the placing plate (8) is provided with a connecting block (9), the top of the connecting block (9) is provided with a vertical rod (901), and the central position of the outer side of the vertical rod (901) is provided with a limiting block (902).
5. A refrigerator according to claim 1 for culturing hematopoietic stem cells, characterized in that: the inner wall of placing case (1) has seted up the guide way, the inside sliding connection of guide way has stopper (902), the top of fixed case (4) is provided with sealed lid (11), second handle (1101) are all installed at the both ends at sealed lid (11) top, the fixed orifices has been seted up to the one end at sealed lid (11) top, the inside cover of fixed orifices is equipped with pole setting (901).
6. A refrigerator according to claim 1 for culturing hematopoietic stem cells, characterized in that: the outer sides of the two ends of the bidirectional threaded rod (15) are provided with moving blocks (1501) in a reverse threaded rotating mode, and the front faces of the moving blocks (1501) are hinged with first transmission rods (16).
7. A refrigerator according to claim 1 for culturing hematopoietic stem cells, characterized in that: locating piece (1701) are all installed at the both ends of bottom plate (17) bottom, the other end and locating piece (1701) of first drive lever (16) are articulated mutually, the linkage hole has been seted up to the inside of placing box (13), the inside bearing in linkage hole rotates and is connected with two-way threaded rod (15), third gear (1502) are installed to the outside that two-way threaded rod (15) extend to linkage hole one end, spring (24) are installed at the top of slider (25), the inner wall fixed connection of the top of spring (24) and fixed case (4).
8. A refrigerator according to claim 1 for culturing hematopoietic stem cells, characterized in that: the bottom of second transfer line (22) one end articulates there is pull rod (23), the removal hole has all been seted up at the both ends of fixed case (4), the inside slip interlude in removal hole is connected with pull rod (23), guide block (2601) are installed in the front of installation piece (26), the restriction hole has been seted up in the front of second transfer line (22), the inside sliding connection in restriction hole has guide block (2601).
9. A refrigerator according to claim 1 for culturing hematopoietic stem cells, characterized in that: the test tube rack is characterized in that a placing groove is formed in the top of the bottom plate (17), a placing hole is formed in the top plate (19), the placing groove is located at the bottom of the placing hole, a test tube (20) is sleeved in the placing hole, and the placing groove is the same as the test tube (20) in size.
10. A cryopreservation method for non-stem cell culture, which is characterized in that: the refrigeration method for culturing the stem-free cells comprises the following steps;
the first step is as follows: the dry ice box (5) is placed in a placing groove of a fixed box (4) and pressed downwards, the dry ice box (5) can be fixed through a fixing assembly, dry ice can enter the placing groove of a placing plate (8) through a discharge hole, and the dry ice sublimes to absorb heat to refrigerate the interior of the placing box (1);
the second step is that: when the test tube (20) is taken, the motor (7) is started, the motor (7) drives the first gear (701) to rotate, the first gear (701) drives the second gear (1201) to rotate, the second gear (1201) drives the one-way threaded rod (12) to rotate, and the placing box (13) is driven to ascend through the one-way threaded rod (12);
the third step: place box (13) in the in-process that rises, third gear (1502) receive the rotation that blocks of rack (1401), and third gear (1502) drive two-way threaded rod (15) and rotate, and two-way threaded rod (15) drive test tube (20) through movable block (1501), first transfer line (16) and locating piece (1701) and rise, and test tube (20) rise to the top of sealed lid (11), and the staff takes test tube (20).
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