CN211522226U - Kit for rapidly separating and culturing umbilical cord mesenchymal stem cells - Google Patents
Kit for rapidly separating and culturing umbilical cord mesenchymal stem cells Download PDFInfo
- Publication number
- CN211522226U CN211522226U CN201922370681.1U CN201922370681U CN211522226U CN 211522226 U CN211522226 U CN 211522226U CN 201922370681 U CN201922370681 U CN 201922370681U CN 211522226 U CN211522226 U CN 211522226U
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- Prior art keywords
- placing groove
- umbilical cord
- cell
- bottle
- box body
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- Expired - Fee Related
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- 210000003954 umbilical cord Anatomy 0.000 title claims abstract description 56
- 210000002901 mesenchymal stem cell Anatomy 0.000 title claims abstract description 13
- 238000012258 culturing Methods 0.000 title claims abstract description 10
- 239000007788 liquid Substances 0.000 claims abstract description 70
- 210000004027 cell Anatomy 0.000 claims abstract description 49
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 36
- 238000004113 cell culture Methods 0.000 claims abstract description 31
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 27
- 238000005406 washing Methods 0.000 claims abstract description 23
- 238000007710 freezing Methods 0.000 claims abstract description 19
- 230000008014 freezing Effects 0.000 claims abstract description 19
- 230000001079 digestive effect Effects 0.000 claims abstract description 16
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 14
- 238000005507 spraying Methods 0.000 claims abstract description 10
- 238000005213 imbibition Methods 0.000 claims abstract description 9
- 239000007921 spray Substances 0.000 claims abstract description 9
- 238000005192 partition Methods 0.000 claims abstract description 3
- 239000003153 chemical reaction reagent Substances 0.000 claims description 33
- 238000004321 preservation Methods 0.000 claims description 5
- 238000000926 separation method Methods 0.000 claims description 3
- 210000000130 stem cell Anatomy 0.000 abstract description 6
- 239000000243 solution Substances 0.000 description 22
- 239000002274 desiccant Substances 0.000 description 6
- 239000003761 preservation solution Substances 0.000 description 5
- 230000029087 digestion Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 210000003981 ectoderm Anatomy 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000003394 haemopoietic effect Effects 0.000 description 1
- 230000003832 immune regulation Effects 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 210000003716 mesoderm Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000001778 pluripotent stem cell Anatomy 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The utility model discloses a kit for rapidly separating and culturing umbilical cord mesenchymal stem cells, which comprises a box body with a hollow upper end opening, wherein the open end of the box body is hinged with a box cover, the box body divides an inner cavity into an umbilical cord collecting and preserving liquid placing groove, an umbilical cord washing liquid placing groove, a cell digestive juice placing groove, a cell freezing and preserving liquid placing groove, a cell culture plate placing groove and a disinfection bottle placing groove through a partition plate; alcohol disinfection bottle has been placed in the disinfection bottle standing groove, and the bottleneck department intercommunication of alcohol disinfection bottle has push type imbibition mechanism, and the upper end intercommunication of the drain pipe of push type imbibition mechanism has the porous spraying shower nozzle that can spray to all around, and the upper end of porous spraying shower nozzle is provided with the briquetting, and when the lid was closed, can push down the briquetting. The utility model discloses an alcohol is to the inside disinfection of kit, replaces ultraviolet ray disinfection's disinfection mode, can not harm the stem cell of cultivateing.
Description
Technical Field
The utility model relates to a medical science experimental apparatus technical field, in particular to quick separation and culture umbilical cord mesenchymal stem cells's kit.
Background
The mesenchymal stem cells are important members of a stem cell family, are derived from mesoderm and ectoderm in early development, belong to pluripotent stem cells, are originally found in bone marrow, and are increasingly concerned by people due to the characteristics of multidirectional differentiation potential, hematopoietic support, stem cell implantation promotion, immune regulation, self-replication and the like.
The utility model discloses a utility model patent with publication number CN209160351U discloses a kit for rapidly separating and culturing umbilical cord mesenchymal stem cells, which comprises a box body, wherein the inner wall of the box body is provided with a rubber plate, the inner wall of the box body is divided into an umbilical cord collecting and preserving liquid placing groove, an umbilical cord washing liquid placing groove, a cell culture plate placing groove, a cell digestive juice placing groove and a cell freezing and preserving liquid placing groove by a clapboard between the rubber plate and the inner wall of the box body, the middle ends of the inner walls of the umbilical cord collecting and preserving liquid placing groove, the umbilical cord washing liquid placing groove, the cell digestive juice placing groove and the cell freezing and preserving liquid placing groove are provided with a middle plate, the middle plate is provided with a through hole, a groove is arranged in the through hole, a spring is arranged in the groove, the spring is provided with a hemispherical block, the two hemispherical blocks are mutually matched, a reagent bottle is clamped, and the shaking of the reagent bottle, reduce the influence of vibration on cells during culture and meet the requirements in actual work. This application adopts the disinfection of ultraviolet ray lamp disinfection, but ultraviolet ray has the kill and kill effect to the cell equally, can pass transparent reagent bottle damage to the stem cell of cultivateing in the disinfection process.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a quick separation and culture umbilical cord mesenchymal stem cells's kit adopts alcohol to the inside disinfection of kit, replaces ultraviolet ray disinfection's disinfection mode, can not harm the stem cell of cultivation.
The above technical purpose of the present invention can be achieved by the following technical solutions: a kit for rapidly separating and culturing umbilical cord mesenchymal stem cells comprises a hollow box body with an opening at the upper end, wherein the opening end of the box body is hinged with a box cover, the box body divides an inner cavity into an umbilical cord collecting and preserving liquid placing groove, an umbilical cord washing liquid placing groove, a cell digestive juice placing groove, a cell freezing and preserving liquid placing groove, a cell culture plate placing groove and a disinfection bottle placing groove through a partition plate, the middle ends of the inner walls of the umbilical cord collecting and preserving liquid placing groove, the umbilical cord washing liquid placing groove, the cell digestive juice placing groove and the cell freezing and preserving liquid placing groove are fixedly connected with an intermediate plate, and each intermediate plate is provided with a plurality of reagent bottle jacks;
alcohol disinfection bottle has been placed in the disinfection bottle standing groove, the bottleneck department intercommunication of alcohol disinfection bottle has push type imbibition mechanism, the upper end intercommunication of the drain pipe of push type imbibition mechanism has the porous spraying shower nozzle that can spray all around, the upper end of porous spraying shower nozzle is provided with the briquetting, when the lid is closed, can with the briquetting pushes down.
By adopting the technical scheme, the kit is taken out of a freezing chamber at the temperature of-20 ℃, a cover plate is opened, the umbilical cord is washed by washing liquid in an umbilical cord washing liquid bottle, then liquid in a cell freezing liquid bottle is unfrozen, then cells are taken out of the umbilical cord, then the umbilical cord collection liquid bottle is used for storing the cells, then the cells are placed on a six-hole cell culture plate, and then nutrient solution sleeved with the cell culture solution is introduced into the six-hole cell culture plate to culture the cells;
after reagent bottles and other instruments are put back into the reagent box, the box cover is covered, the box cover presses the pressing block downwards, so that the liquid outlet pipe is pressed into the pressing type liquid suction mechanism, the pressing type liquid suction mechanism sucks alcohol from the alcohol disinfection bottle to be sprayed out from the porous spraying nozzle, the sprayed alcohol is scattered into the reagent box to be disinfected in the alcohol box, and after the box cover is opened next time, the liquid outlet pipe is not pressed and automatically extends out of the pressing type liquid suction mechanism.
The utility model discloses a further set up to: the bottom surface of the box body is provided with a plurality of through holes, the lower end of the box body is provided with a drawer box with an upper end opening and matched with the bottom surface of the box body, the drawer box is provided with a drawer capable of being drawn out outwards, and a drying agent is placed in the drawer.
By adopting the technical scheme, alcohol and moisture in the disinfected reagent kit are absorbed by the drying agent, so that the reagent kit is kept dry.
The utility model discloses a further set up to: the box body is connected with one side of the box cover far away from the hinge joint through a pull buckle.
The utility model discloses a further set up to: the umbilical cord is gathered and is preserved liquid standing groove and put the umbilical cord through the reagent bottle jack and gather the preservation liquid bottle, the umbilical cord washing liquid standing groove has put the umbilical cord washing liquid bottle through the reagent bottle jack, the cell digestive juice standing groove has put the cell digestive juice bottle through the reagent bottle jack, the cell is frozen and is deposited the liquid standing groove and has put the cell and freeze the preservation liquid bottle through the reagent bottle jack, put the cell culture liquid suit in the cell culture liquid standing groove, six hole cell culture boards have been put in the cell culture board standing groove.
To sum up, the utility model discloses following beneficial effect has: firstly, the utility model adopts the pressing type liquid suction mechanism to suck alcohol from the alcohol disinfection bottle for spray disinfection, and can disinfect once when closing the box cover, thereby avoiding that the worker needs to spray alcohol for disinfection before putting back the reagent bottle each time, and simultaneously replacing the existing ultraviolet disinfection mode, and effectively preventing the cultured stem cells from being damaged during disinfection;
and secondly, a drying agent is placed at the bottom of the reagent box, so that alcohol and water used for disinfection in the reagent box can be absorbed, and the inside of the reagent box is effectively kept dry.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention;
fig. 2 is a partial sectional view for showing an internal structure.
In the figure: 1. a box body; 2. a box cover; 3. pulling the button; 41. umbilical cord collection and preservation solution placing groove; 42. umbilical cord washing liquid placing groove; 43. placing a cell digestive juice placing groove; 44. placing a cell freezing solution in a groove; 45. a cell culture solution placing groove; 46. a cell culture plate placing groove; 47. a disinfection bottle placing groove; 5. a middle plate; 51. a reagent bottle jack; 61. collecting and preserving liquid bottles for umbilical cords; 62. umbilical cord washing liquid bottle; 63. a cell digestive juice bottle; 64. a cell cryopreservation liquid bottle; 65. sleeving a cell culture solution; 66. six-well cell culture plates; 7. alcohol disinfection bottle; 71. a pressing type liquid suction mechanism; 72. a liquid outlet pipe; 73. a porous spray nozzle; 74. briquetting; 8. a through hole; 9. a drawer box; 91. a drawer.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
Example, a kit for rapidly separating and culturing umbilical cord mesenchymal stem cells, refer to fig. 1-2, comprising a box body 1 with an open upper end, a box cover 2 hinged to the open end of the box body 1, a zipper 3 connecting the box body 1 and the box cover 2 at the side far away from the hinged side, a baffle separating the inner cavity of the box body 1 into an umbilical cord collection and preservation solution placement groove 41, an umbilical cord washing solution placement groove 42, a cell digestive juice placement groove 43, a cell freezing storage solution placement groove 44, a cell culture solution placement groove 45, a cell culture plate placement groove 46 and a disinfection bottle placement groove 47, wherein the umbilical cord collection and preservation solution placement groove 41, the umbilical cord washing solution placement groove 42, the cell digestive juice placement groove 43 and the cell freezing storage solution placement groove 44 are distributed in four grids, the cell culture solution placement groove 45 and the cell culture plate placement groove 46 are arranged at one side of the four grids, the disinfection bottle placement groove 47 is arranged at the middle cross of the four grids, the umbilical cord is gathered and is preserved liquid standing groove 41, umbilical cord washing liquid standing groove 42, cell digestive juice standing groove 43 and cell and freeze and deposit liquid standing groove 44 inner wall middle-end fixedly connected with intermediate plate 5, and a plurality of reagent bottle jack 51 have all been seted up to each intermediate plate 5.
An umbilical cord collection and preservation solution bottle 61 is placed in the umbilical cord collection and preservation solution placing groove 41 through a reagent bottle jack 51, an umbilical cord washing solution bottle 62 is placed in the umbilical cord washing solution placing groove 42 through the reagent bottle jack 51, a cell digestion solution bottle 63 is placed in the cell digestion solution placing groove 43 through the reagent bottle jack 51, a cell freezing solution bottle 64 is placed in the cell freezing solution placing groove 44 through the reagent bottle jack 51, a cell culture solution suit 65 is placed in the cell culture solution placing groove 45, and a six-hole cell culture plate 66 is placed in the cell culture plate placing groove 46.
Alcohol disinfection bottle 7 has been placed in disinfection bottle standing groove 47, the bottleneck department of alcohol disinfection bottle 7 intercommunication has a push type imbibition mechanism 71, the upper end intercommunication of the drain pipe 72 of push type imbibition mechanism 71 has a porous spraying nozzle 73 that can spray to all around, the upper end of porous spraying nozzle 73 is provided with a briquetting 74, when lid 2 is closed, can push down briquetting 74, make drain pipe 72 impress push type imbibition mechanism 71 to spout alcohol suction from porous spraying nozzle 73.
A plurality of through holes 8 are formed in the bottom surface of the box body 1, a drawer box 9 which is provided with an opening at the upper end and is matched with the bottom surface of the box body 1 is arranged at the lower end of the box body 1, a drawer 91 which can be drawn out outwards is arranged on the drawer box 9, drying agents are placed in the drawer 91, and alcohol and moisture in the disinfected reagent box are absorbed through the drying agents to keep the inside of the reagent box dry.
The use method comprises the following steps: taking the kit out of a freezing chamber at the temperature of 20 ℃ below zero, opening a cover plate through a pull buckle 3, washing the umbilical cord by using a washing liquid in an umbilical cord washing liquid bottle 62, then thawing a cell freezing liquid in a cell freezing liquid bottle 64, then taking the cell out of the umbilical cord, then collecting a preservation liquid bottle 61 by using the umbilical cord to preserve the cell, then placing the cell on a six-hole cell culture plate 66, then introducing a nutrient solution of a cell culture solution set 65 into the six-hole cell culture plate 66, and culturing the cell;
after reagent bottles and other instruments are put back into the reagent kit, the box cover 2 is covered, the box cover 2 presses the press block 74 downwards to press the liquid outlet pipe 72 into the press type liquid suction mechanism 71, the press type liquid suction mechanism 71 sucks alcohol from the alcohol disinfection bottle 7 to spray out from the porous spray nozzle 73, the sprayed alcohol is scattered into the reagent kit to disinfect the interior of the alcohol kit, the alcohol slowly passes through the through hole 8 formed in the bottom surface of the box body 1 to be absorbed and dried by the drying agent in the drawer 91, and after the box cover 2 is opened next time, the liquid outlet pipe 72 automatically extends out of the press type liquid suction mechanism 71 without being pressed.
The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications to the present embodiment without inventive contribution as required after reading the present specification, but all of them are protected by patent laws within the scope of the claims of the present invention.
Claims (4)
1. The utility model provides a kit of rapid separation and culture umbilical cord mesenchymal stem cell, includes cavity upper end open-ended box body (1), its characterized in that: the open end of the box body (1) is hinged with a box cover (2), the box body (1) divides an inner cavity into an umbilical cord collecting and preserving liquid placing groove (41), an umbilical cord washing liquid placing groove (42), a cell digestive juice placing groove (43), a cell freezing preservation liquid placing groove (44), a cell culture liquid placing groove (45), a cell culture plate placing groove (46) and a disinfection bottle placing groove (47) through a partition plate, the middle ends of the inner walls of the umbilical cord collecting and preserving liquid placing groove (41), the umbilical cord washing liquid placing groove (42), the cell digestive juice placing groove (43) and the cell freezing preservation liquid placing groove (44) are fixedly connected with an intermediate plate (5), and each intermediate plate (5) is provided with a plurality of reagent bottle jacks (51);
alcohol disinfection bottle (7) have been placed in disinfection bottle standing groove (47), the bottleneck department intercommunication of alcohol disinfection bottle (7) has push type imbibition mechanism (71), the upper end intercommunication of drain pipe (72) of push type imbibition mechanism (71) has porous spraying shower nozzle (73) that can spray all around, the upper end of porous spraying shower nozzle (73) is provided with briquetting (74), when lid (2) are closed, can with briquetting (74) push down.
2. The kit for rapidly isolating and culturing umbilical cord mesenchymal stem cells according to claim 1, wherein: a plurality of through-holes (8) have been seted up to the bottom surface of box body (1), the lower extreme of box body (1) be provided with the upper end opening and with box body (1) bottom surface matched with drawer box (9), drawer box (9) are provided with drawer (91) that can outwards take out, the drier has been placed in drawer (91).
3. The kit for rapidly isolating and culturing umbilical cord mesenchymal stem cells according to claim 1, wherein: the box body (1) is connected with one side, far away from the hinge joint, of the box cover (2) through a pull buckle (3).
4. The kit for rapidly isolating and culturing umbilical cord mesenchymal stem cells according to claim 1, wherein: the umbilical cord collecting and preserving liquid placing groove (41) is placed with an umbilical cord collecting and preserving liquid bottle (61) through a reagent bottle jack (51), the umbilical cord washing liquid placing groove (42) is placed with an umbilical cord washing liquid bottle (62) through the reagent bottle jack (51), the cell digestive liquid placing groove (43) is placed with a cell digestive liquid bottle (63) through the reagent bottle jack (51), the cell freezing and preserving liquid placing groove (44) is placed with a cell freezing and preserving liquid bottle (64) through the reagent bottle jack (51), a cell culture liquid suit (65) is placed in the cell culture liquid placing groove (45), and a six-hole cell culture plate (66) is placed in the cell culture plate placing groove (46).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922370681.1U CN211522226U (en) | 2019-12-25 | 2019-12-25 | Kit for rapidly separating and culturing umbilical cord mesenchymal stem cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922370681.1U CN211522226U (en) | 2019-12-25 | 2019-12-25 | Kit for rapidly separating and culturing umbilical cord mesenchymal stem cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211522226U true CN211522226U (en) | 2020-09-18 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201922370681.1U Expired - Fee Related CN211522226U (en) | 2019-12-25 | 2019-12-25 | Kit for rapidly separating and culturing umbilical cord mesenchymal stem cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211522226U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112538416A (en) * | 2020-12-27 | 2021-03-23 | 弘康信达(江苏)医学研究有限公司 | Kit for rapidly separating and culturing umbilical cord mesenchymal stem cells and use method thereof |
| CN112574855A (en) * | 2020-12-27 | 2021-03-30 | 弘康信达(江苏)医学研究有限公司 | Device and method for separating and culturing animal bone marrow mesenchymal stem cells |
| CN112625904A (en) * | 2020-12-27 | 2021-04-09 | 弘康信达(江苏)医学研究有限公司 | Isolated culture equipment and method for ectodermal mesenchymal stem cells |
-
2019
- 2019-12-25 CN CN201922370681.1U patent/CN211522226U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112538416A (en) * | 2020-12-27 | 2021-03-23 | 弘康信达(江苏)医学研究有限公司 | Kit for rapidly separating and culturing umbilical cord mesenchymal stem cells and use method thereof |
| CN112574855A (en) * | 2020-12-27 | 2021-03-30 | 弘康信达(江苏)医学研究有限公司 | Device and method for separating and culturing animal bone marrow mesenchymal stem cells |
| CN112625904A (en) * | 2020-12-27 | 2021-04-09 | 弘康信达(江苏)医学研究有限公司 | Isolated culture equipment and method for ectodermal mesenchymal stem cells |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200918 |