CN112574855A - Device and method for separating and culturing animal bone marrow mesenchymal stem cells - Google Patents

Device and method for separating and culturing animal bone marrow mesenchymal stem cells Download PDF

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CN112574855A
CN112574855A CN202011571769.0A CN202011571769A CN112574855A CN 112574855 A CN112574855 A CN 112574855A CN 202011571769 A CN202011571769 A CN 202011571769A CN 112574855 A CN112574855 A CN 112574855A
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box body
culture
stem cells
mesenchymal stem
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张金芳
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Hongkang Xinda Jiangsu Medical Research Co ltd
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Abstract

The invention discloses a device and a method for separating and culturing animal bone marrow mesenchymal stem cells, wherein the containing space is sealed by a box cover, the damping device enables a distribution device to be installed more stably, the monitoring device monitors the temperature in the box body, the sterilization device sterilizes the interior of the box body, a slide rail is installed in the containing space, a slide block can slide on the slide rail and drive a containing box to slide on the slide rail, the box cover is opened, an auxiliary plate is connected in the box body through a pin shaft, the auxiliary plate is opened and the containing box is pushed out, the containing box is used for containing required reagent bottles, instruments and the like, the space for taking the reagent bottles and the like is enlarged by adopting a drawing mode, the operation is more convenient, and the using effect is better.

Description

Device and method for separating and culturing animal bone marrow mesenchymal stem cells
Technical Field
The invention relates to the technical field of medical experimental instruments, in particular to a device and a method for separating and culturing animal bone marrow mesenchymal stem cells.
Background
Mesenchymal stem cells are important members of a stem cell family, are derived from mesoderm in an early development stage, and belong to pluripotent stem cells. The mesenchymal stem cells are clinically applied to solving various blood system diseases, cardiovascular diseases, liver cirrhosis, nervous system diseases, partial resection injury repair of knee joint meniscus, autoimmune diseases and the like, and great breakthrough is made, so that lives of more patients are saved; in addition, mesenchymal stem cells have long-term development prospects in nervous system repair and more aspects.
At present, animal bone marrow mesenchymal stem cells are mostly separated and cultured by adopting a mesenchymal stem cell rapid separation and culture kit, and the kit is a box for containing chemical reagents for monitoring chemical components, drug residues, virus types and the like, and is convenient for cell culture. However, the existing reagent kit only has a simple fixing device for placing reagent bottles and the like, and has limited accommodating space, inconvenient operation and poor use effect.
Disclosure of Invention
The invention aims to provide a device and a method for separating and culturing animal bone marrow mesenchymal stem cells, and aims to solve the technical problems of inconvenient operation and poor use effect caused by simple structure and limited accommodating space of a kit in the prior art.
In order to achieve the purpose, the device for separating and culturing the animal bone marrow mesenchymal stem cells comprises a box body, a box cover, an auxiliary plate, a damping device, a distribution device, a sterilization device and a monitoring device;
the box cover is rotatably connected with the box body and is positioned on one side of the box body; one side of the auxiliary plate is rotatably connected with the box body and is positioned on one side of the box body, which is far away from the box cover, and the other side of the auxiliary plate is detachably connected with the box body and forms an accommodating space with the box body; the damping device is fixedly connected with the box body, the sterilizing device is fixedly connected with the box body, and the monitoring device is fixedly connected with the box body;
the distribution device comprises a slide rail, a slide block and a storage box, and the slide rail is fixedly connected with the box body and is positioned in the accommodating space; one side of slider with slide rail sliding connection to be located the slide rail is kept away from one side of box body, the opposite side of slider with receiver fixed connection.
Wherein, distributor still includes base plate and baffle, the base plate with the receiver can be dismantled and be connected, and be located the receiver is kept away from one side of slider, the baffle with the base plate block, and be located the base plate is kept away from one side of receiver.
The base plate is provided with a mounting groove, and the mounting groove is positioned on one side, close to the partition plate, of the base plate.
The distribution device further comprises a storage plate, wherein the storage plate is clamped with the box body and is positioned on one side, close to the storage box, of the box body.
The object placing plate is provided with a through hole, and the through hole is located on one side, far away from the box body, of the object placing plate.
The device for the isolated culture of the animal bone marrow mesenchymal stem cells further comprises a handle, wherein the handle is fixedly connected with the box body and is positioned on one side of the box body, which is far away from the box cover.
The device for isolated culture of the animal bone marrow mesenchymal stem cells further comprises a fixing lock, one side of the fixing lock is fixedly connected with the box body and is positioned on one side of the box body close to the auxiliary plate, and the other side of the fixing lock is detachably connected with the box cover.
A method for separating and culturing animal bone marrow mesenchymal stem cells comprises the following steps:
collecting animal bone marrow cell tissue under aseptic condition, placing into a reagent bottle filled with cell preservation solution, and transporting to a laboratory in a sealed manner;
washing animal bone marrow cell tissue with a washing solution;
cutting the cleaned animal bone marrow cell tissue into 1-2mm3Uniformly spreading the tissue fragments on the bottom of a culture dish or a culture bottle, and standing for 5-15 minutes until the tissue fragments are tightly attached to the culture dish or the culture bottle;
adding the prepared whole culture medium into a culture dish or a culture bottle until the tissue fragments are wet;
placing the culture dish or culture bottle added with the full culture medium into a 37 ℃ incubator with the carbon dioxide concentration of 5% for culture, replacing the full culture medium once every 1 to 3 days, and culturing for 5 to 10 days to obtain the P0 generation mesenchymal stem cells;
continuously culturing for 10 to 14 days, when the cell fusion rate in a culture dish or a culture bottle reaches 50 to 70 percent, cleaning twice by using a cleaning solution, simultaneously adding a digestive juice, shaking the culture dish or the culture bottle to ensure that the digestive juice is fully contacted with the cells, and continuously placing the culture dish or the culture bottle into an incubator for digestion for 30 to 60 seconds;
adding the prepared full culture medium into a culture dish or a culture bottle after digestion to terminate digestion, namely obtaining the mesenchymal stem cells of P1 generation, taking out a part of the mesenchymal stem cells of P1 generation, putting the part of the mesenchymal stem cells of P1 generation into a frozen stock solution for freezing storage, and adding the rest of the mesenchymal stem cells of P1 generation into the frozen stock solution according to the requirement of 1: subculturing at a ratio of 3.
The device and the method for isolated culture of the animal bone marrow mesenchymal stem cells have the advantages that the accommodating space is formed by the box body and the auxiliary plate, the accommodating space is sealed by the box cover, the distribution device is more stably installed by the damping device, the monitoring device monitors the temperature inside the box body, the sterilization device sterilizes the inside of the box body, the slide rail is installed in the accommodating space, the slide block and the slide rail are oppositely arranged in a matching way, can slide on the slide rail and are fixedly connected with the storage box to drive the storage box to slide on the slide rail, the box cover consists of two parts which are respectively connected through a pin shaft, can rotate on the box body, the box cover is opened, the auxiliary plate is connected in the box body through the pin shaft to further open the auxiliary plate and push out the storage box, the receiver is in the same place required reagent bottle and apparatus etc. adopts the mode of taking out, enlarges the space of taking reagent bottle etc. and it is more convenient to operate, and the result of use is better.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural diagram of an apparatus for isolated culture of mesenchymal stem cells of animal bone marrow according to the present invention.
Fig. 2 is a schematic view showing a structure in which the auxiliary plate of the present invention is opened.
Fig. 3 is a schematic structural view of the storage plate of the present invention.
Fig. 4 is an enlarged view of the invention at B of fig. 2.
Fig. 5 is an enlarged view of fig. 3 a of the present invention.
FIG. 6 is a diagram illustrating the steps of a method for culturing an isolated mesenchymal stem cell of an animal according to the present invention.
1-box body, 2-box cover, 3-auxiliary plate, 4-handle, 5-fixed lock, 10-shock absorption device, 20-distribution device, 21-slide rail, 22-slide block, 23-storage box, 24-base plate, 25-partition plate, 26-storage plate, 30-sterilization device, 31-disinfectant bottle, 32-spray nozzle, 33-extrusion mechanism, 40-monitoring device, 41-temperature sensor, 42-display screen, 100-device for separating and culturing animal bone marrow mesenchymal stem cells, 101-containing space, 241-mounting groove and 261-through hole.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention.
In the description of the present invention, it is to be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships illustrated in the drawings, and are used merely for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, are not to be construed as limiting the present invention. Further, in the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
Referring to fig. 1 to 5, the present invention provides an apparatus 100 for isolated culture of mesenchymal stem cells of animal bone marrow, comprising a box body 1, a box cover 2, an auxiliary plate 3, a shock-absorbing device 10, a distribution device 20, a sterilization device 30 and a monitoring device 40;
the box cover 2 is rotatably connected with the box body 1 and is positioned on one side of the box body 1; one side of the auxiliary plate 3 is rotatably connected with the box body 1 and is positioned on one side of the box body 1 far away from the box cover 2, and the other side of the auxiliary plate 3 is detachably connected with the box body 1 and forms an accommodating space 101 with the box body 1; the damping device 10 is fixedly connected with the box body 1, the sterilizing device 30 is fixedly connected with the box body 1, and the monitoring device 40 is fixedly connected with the box body 1;
the distribution device 20 comprises a slide rail 21, a slide block 22 and a storage box 23, wherein the slide rail 21 is fixedly connected with the box body 1 and is positioned in the accommodating space 101; one side of slider 22 with slide rail 21 sliding connection to be located slide rail 21 keeps away from one side of box body 1, the opposite side of slider 22 with receiver 23 fixed connection.
In this embodiment, the box body 1 and the auxiliary plate 3 form the accommodating space 101, the box cover 2 seals the accommodating space 101, the shock absorbing device 10 enables the distribution device 20 to be installed more stably, the monitoring device 40 monitors the temperature inside the box body 1, the sterilization device 30 sterilizes the inside of the box body 1, the slide rail 21 is installed in the accommodating space 101, the slider 22 is arranged opposite to the slide rail 21, can slide on the slide rail 21 and is fixedly connected with the storage box 23 to drive the storage box 23 to slide on the slide rail 21, the box cover 2 is composed of two parts, respectively connected by a pin shaft and can rotate on the box body 1, the box cover 2 is opened, the auxiliary plate 3 is connected in the box body 1 by the pin shaft, and the shock absorbing device 10 is connected to a side close to the storage box 23, the auxiliary plate 3 is further opened to push out the storage box 23, the auxiliary plate 3 and the damping device 10 support the retracted storage box 23, so that the storage box 23 is used more stably, the storage box 23 is used for placing required reagent bottles, instruments and the like, the space for taking the reagent bottles and the like is enlarged by adopting a pulling-out mode, the operation is more convenient, and the using effect is better.
Further, referring to fig. 2 and 5, the dispensing device 20 further includes a base plate 24 and a partition plate 25, the base plate 24 is detachably connected to the storage box 23 and is located on a side of the storage box 23 away from the slider 22, and the partition plate 25 is engaged with the base plate 24 and is located on a side of the base plate 24 away from the storage box 23.
In this embodiment, base plate 24 is installed in the bottom of receiver 23, the baffle 25 block is in on the base plate 24, and the quantity of baffle 25 is a plurality of, with base plate 24 with receiver 23 forms the separate space, conveniently places required reagent bottle and apparatus etc. and avoids mixed pollution, uses safelyr, and the result of use is better.
Further, referring to fig. 5, the substrate 24 has a mounting groove 241, and the mounting groove 241 is located on a side of the substrate 24 close to the partition 25.
In the present embodiment, the mounting grooves 241 of the base plate 24 are provided opposite to the partition plate 25 to engage with the partition plate 25, and the number of the mounting grooves 241 is plural and distributed on the surface wall of the base plate 24, so that the partition space formed by the partition plate 25 can be adjusted as required, and the operation is more convenient and the use feeling is better.
Further, referring to fig. 3, the dispensing device 20 further includes a storage plate 26, and the storage plate 26 is engaged with the box body 1 and is located on a side of the box body 1 close to the storage box 23.
In this embodiment, three sides of the object placing plate 26 are engaged with the box body 1, and the number of the object placing plate is plural, and the object placing plate is distributed along the height direction of the box body 1, so that the accommodating space of the box body 1 is increased, and the using effect is better.
Further, referring to fig. 3, the object placing plate 26 has a through hole 261, and the through hole 261 is located on a side of the object placing plate 26 away from the box body 1.
In this embodiment, put and have on the thing board 26 through-hole 261, be convenient for spray disinfection reagent and fall into put the below of thing board 26, and then can disinfect comprehensively, be favorable to the cultivation of cell.
Further, referring to fig. 1, the apparatus 100 for isolated culturing of animal mesenchymal stem cells further includes a handle 4, wherein the handle 4 is fixedly connected to the box body 1 and is located at a side of the box body 1 away from the box cover 2.
In this embodiment, the handle 4 is arranged on the box body 1, and is used for touching the handle 4 to lift the box body 1, so that the use is more convenient, and the user experience is better.
Further, referring to fig. 1, the apparatus 100 for isolated culture of mesenchymal stem cells of animal bone marrow further includes a fixing lock 5, one side of the fixing lock 5 is fixedly connected to the box body 1 and is located at one side of the box body 1 close to the auxiliary plate 3, and the other side of the fixing lock 5 is detachably connected to the box cover 2.
In this embodiment, the fixing lock 5 is used for locking the box cover 2, the box cover 2 is composed of two parts which are respectively connected through a pin shaft and can rotate on the box body 1, and the box body 1 is sealed by fixing the fixing lock 5 on the box body 1, so that the internal reagent bottles, instruments and the like can be placed more safely.
Further, referring to fig. 2, the monitoring device 40 includes a temperature sensor 41 and a display screen 42, the temperature sensor 41 is located inside the box body 1, and the display screen 42 is fixedly connected to the box body 1, located outside the box body 1, and penetrates through the box body 1.
In this embodiment, the model of the temperature sensor 41 is PT100, which senses the temperature inside the cartridge body 1 and transmits the display screen 42, the model of the display screen 42 is 275M1RZ, which displays the temperature sensed by the temperature sensor 41 on the outer side of the cartridge body 1, so as to facilitate observation and further facilitate the use of cell culture.
Further, referring to fig. 1 and 2, the sterilization device 30 includes a disinfectant bottle 31, a spray nozzle 32 and a squeezing mechanism 33, the disinfectant bottle 31 is fixedly connected with the box cover 2, is located on one side of the box cover 2 away from the box body 1, and penetrates through the box cover 2; the spray nozzle 32 is fixedly connected with the disinfectant bottle 31 and is positioned on one side of the disinfectant bottle 31 close to the box body 1; the squeezing mechanism 33 is fixedly connected with the disinfectant bottle 31 and is positioned on one side of the disinfectant bottle 31 far away from the box cover 2.
In this embodiment, disinfectant such as alcohol is equipped with in the disinfectant bottle 31, installs the outside of lid 2 is run through lid 2 with spray nozzle 32 connects, squeezing mechanism 33 comprises squeeze bar and piston, and the squeeze bar is connected with the piston, and the piston is attached the inside of disinfectant bottle 31, through pressing the squeeze bar, the piston is in reciprocate in disinfectant bottle 31, according to the pressure differential principle, will disinfectant in the disinfectant bottle 31 passes through spray nozzle 32 sprays the inside of box body 1 is right the inside of box body 1 is disinfected, is favorable to cell culture's efficiency.
Referring to fig. 6, a method for isolating and culturing mesenchymal stem cells of animal bone marrow comprises the following steps:
s101: collecting animal bone marrow cell tissue under aseptic condition, placing into a reagent bottle filled with cell preservation solution, and sealing, transporting and testing in laboratory.
S102: animal bone marrow cell tissue was washed with a washing solution.
S103: cutting cleaned animal bone marrow cell tissue into 1-2mm3The tissue fragments are evenly spread at the bottom of a culture dish or a culture bottle and are placed for 5 to 15 minutes until the tissue fragments are tightly attached to the culture dish or the culture bottle.
S104: the prepared whole culture medium is added into a culture dish or a culture bottle, and the tissue fragment is wet.
S105: placing the culture dish or culture bottle with the whole culture medium into a 37 ℃ incubator with the carbon dioxide concentration of 5% for culture, replacing the whole culture medium once every 1-3 days, and culturing for-5-10 days to obtain the P0 generation mesenchymal stem cells.
S106: and continuously culturing for-10-14 days, when the cell fusion rate in the culture dish or the culture bottle reaches 50-70%, cleaning twice by using a cleaning solution, simultaneously adding a digestive juice, shaking the culture dish or the culture bottle to ensure that the digestive juice is fully contacted with the cells, and continuously placing the culture dish or the culture bottle into an incubator for digestion for 30-60 seconds.
S107: adding the prepared full culture medium into a culture dish or a culture bottle after digestion to terminate digestion, namely obtaining the mesenchymal stem cells of P1 generation, taking out a part of the mesenchymal stem cells of P1 generation, putting the part of the mesenchymal stem cells of P1 generation into a frozen stock solution for freezing storage, and adding the rest of the mesenchymal stem cells of P1 generation into the frozen stock solution according to the requirement of 1: subculturing at a ratio of 3.
In this embodiment, the lid 2 is opened, the animal bone marrow cell tissue is stored in a reagent bottle of cell preservation solution placed in the dispensing device 20, the lid 2 is closed to seal the case 1, the lid 2 is opened after transportation and laboratory, the washing solution is taken out to wash the animal bone marrow cell tissue, and the washed animal bone marrow cell tissue is cut into 1 to 2mm pieces by an instrument placed in the dispensing device 203Uniformly paving the tissue fragments at the bottom of a culture dish or a culture bottle, placing for 5-15 minutes until the tissue fragments cling to the culture dish or the culture bottle, and mixing the tissue fragments with the weight ratio of 1: 0.5-0.8: dissolving 0.8-1:0.8-1 of culture solution, additives, growth factor I and growth factor II in water for injection to prepare a full culture medium, adding the full culture medium into a culture dish or a culture bottle, wetting broken tissue, putting the culture dish or the culture bottle into a 37 ℃ incubator with the carbon dioxide concentration of 5% for culture, replacing the full culture medium once every 1-3 days, and culturing for-5-10 days to obtain the P0-generation mesenchymal stem cells; continuously culturing for-10-14 days, when the cell fusion rate in the culture dish or the culture bottle reaches 50-70%, cleaning twice by using a cleaning solution, simultaneously adding a digestive juice, shaking and shaking the culture dish or the culture bottle to make the digestive juice fully contact with the cells, and continuously placing the culture dish or the culture bottle into an incubator for digesting for 30-60 seconds; adding the prepared full culture medium into the digested culture dish or culture bottle to terminate the digestion, thus obtaining the mesenchymal stem cells of the P1 generation, and taking out a part of the mesenchymal stem cells of the P1 generationAnd (3) putting the cells into a freezing storage solution for freezing storage, wherein the freezing storage density of the freezing storage solution is 3-5 multiplied by 106 cells/ml, and the rest of the P1 generation mesenchymal stem cells are cultured according to the proportion of 1: 3, and the cell culture operation is more convenient and faster by utilizing the box body 1.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

Claims (8)

1. A device for separating and culturing animal bone marrow mesenchymal stem cells is characterized by comprising a box body, a box cover, an auxiliary plate, a damping device, a distribution device, a sterilization device and a monitoring device;
the box cover is rotatably connected with the box body and is positioned on one side of the box body; one side of the auxiliary plate is rotatably connected with the box body and is positioned on one side of the box body, which is far away from the box cover, and the other side of the auxiliary plate is detachably connected with the box body and forms an accommodating space with the box body; the damping device is fixedly connected with the box body, the sterilizing device is fixedly connected with the box body, and the monitoring device is fixedly connected with the box body;
the distribution device comprises a slide rail, a slide block and a storage box, and the slide rail is fixedly connected with the box body and is positioned in the accommodating space; one side of slider with slide rail sliding connection to be located the slide rail is kept away from one side of box body, the opposite side of slider with receiver fixed connection.
2. The apparatus for isolated culture of mesenchymal stem cells of an animal according to claim 1,
the distribution device further comprises a base plate and a partition plate, the base plate is detachably connected with the storage box and located on one side, away from the sliding block, of the storage box, and the partition plate is clamped with the base plate and located on one side, away from the storage box, of the base plate.
3. The apparatus for isolated culture of mesenchymal stem cells of an animal according to claim 2,
the base plate is provided with a mounting groove, and the mounting groove is positioned on one side, close to the partition plate, of the base plate.
4. The apparatus for isolated culture of mesenchymal stem cells of an animal according to claim 1,
the distribution device further comprises a storage plate, wherein the storage plate is clamped with the box body and is positioned on one side, close to the storage box, of the box body.
5. The apparatus for isolated culture of mesenchymal stem cells of an animal according to claim 4,
the object placing plate is provided with a through hole, and the through hole is positioned on one side of the object placing plate, which is far away from the box body.
6. The apparatus for isolated culture of mesenchymal stem cells of an animal according to claim 1,
the device for isolated culture of the animal bone marrow mesenchymal stem cells further comprises a handle, wherein the handle is fixedly connected with the box body and is positioned on one side of the box body, which is far away from the box cover.
7. The apparatus for isolated culture of mesenchymal stem cells of an animal according to claim 1,
the device for isolated culture of the animal bone marrow mesenchymal stem cells further comprises a fixing lock, one side of the fixing lock is fixedly connected with the box body and is positioned at one side of the box body close to the auxiliary plate, and the other side of the fixing lock is detachably connected with the box cover.
8. A method for separating and culturing animal bone marrow mesenchymal stem cells is characterized by comprising the following steps:
collecting animal bone marrow cell tissue under aseptic condition, placing into a reagent bottle filled with cell preservation solution, and transporting to a laboratory in a sealed manner;
washing animal bone marrow cell tissue with a washing solution;
cutting the cleaned animal bone marrow cell tissue into 1-2mm3Uniformly spreading the tissue fragments on the bottom of a culture dish or a culture bottle, and standing for 5-15 minutes until the tissue fragments are tightly attached to the culture dish or the culture bottle;
adding the prepared whole culture medium into a culture dish or a culture bottle until the tissue fragments are wet;
placing the culture dish or culture bottle added with the full culture medium into a 37 ℃ incubator with the carbon dioxide concentration of 5% for culture, replacing the full culture medium once every 1 to 3 days, and culturing for 5 to 10 days to obtain the P0 generation mesenchymal stem cells;
continuously culturing for 10 to 14 days, when the cell fusion rate in a culture dish or a culture bottle reaches 50 to 70 percent, cleaning twice by using a cleaning solution, simultaneously adding a digestive juice, shaking the culture dish or the culture bottle to ensure that the digestive juice is fully contacted with the cells, and continuously placing the culture dish or the culture bottle into an incubator for digestion for 30 to 60 seconds;
adding the prepared full culture medium into a culture dish or a culture bottle after digestion to terminate digestion, namely obtaining the mesenchymal stem cells of P1 generation, taking out a part of the mesenchymal stem cells of P1 generation, putting the part of the mesenchymal stem cells of P1 generation into a frozen stock solution for freezing storage, and adding the rest of the mesenchymal stem cells of P1 generation into the frozen stock solution according to the requirement of 1: subculturing at a ratio of 3.
CN202011571769.0A 2020-12-27 2020-12-27 Device and method for separating and culturing animal bone marrow mesenchymal stem cells Pending CN112574855A (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN206872855U (en) * 2017-07-07 2018-01-12 李雪英 A kind of kit of quick separating and culture umbilical cord mesenchymal stem cells
CN210506375U (en) * 2019-06-17 2020-05-12 河北三臧生物科技有限公司 Be convenient for placenta tissue stem cell with cultivating kit
CN211522226U (en) * 2019-12-25 2020-09-18 中国人民解放军联勤保障部队第九二〇医院 Kit for rapidly separating and culturing umbilical cord mesenchymal stem cells

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN206872855U (en) * 2017-07-07 2018-01-12 李雪英 A kind of kit of quick separating and culture umbilical cord mesenchymal stem cells
CN210506375U (en) * 2019-06-17 2020-05-12 河北三臧生物科技有限公司 Be convenient for placenta tissue stem cell with cultivating kit
CN211522226U (en) * 2019-12-25 2020-09-18 中国人民解放军联勤保障部队第九二〇医院 Kit for rapidly separating and culturing umbilical cord mesenchymal stem cells

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