CN211445694U - Separator for menstrual blood stem cells - Google Patents

Abstract

The utility model relates to the technical field of stem cell separation, in particular to a separator for menstrual blood stem cells, which comprises a separator body, wherein the top of the separator body is provided with a sealing cover, one side of the top of the sealing cover is provided with a menstrual blood inlet, the bottom of the separator body is provided with a plasma outlet, the inside of the separator body is provided with a plurality of mutually communicated separating chambers, the insides of the separating chambers are respectively provided with a filter screen, the inside of the separator body is provided with a stirring device which passes through the separating chambers and the filter screen, and the stirring device is rotationally connected with the sealing cover; the outside of the separator body is provided with a heating device. Adopt the separator of above-mentioned structure, realized the separation to endometrial tissue, stem cell, erythrocyte and leucocyte in menstrual blood, can promote the mobility of blood through agitating unit when separating moreover, can prevent through heating device that blood solidifies, prevents that endometrial tissue from adsorbing blood, has improved separation efficiency greatly, easy operation moreover.

Description

Separator for menstrual blood stem cells

Technical Field

The utility model relates to a stem cell separation technical field especially relates to a separator for menstrual blood stem cell.

Background

Stem cells are a type of pluripotent cells with self-replicating ability, and can be divided into embryonic stem cells and adult stem cells according to the development stage of the stem cells; the development potential of stem cells can be divided into: totipotent, pluripotent and unipotent stem cells. Stem cells are insufficiently differentiated and immature cells, have potential functions of regenerating various organs and human bodies, and are called as universal cells in the medical field.

The endometrium of the female is a highly regenerative tissue, and a small group of stem cells with strong proliferation and multidirectional differentiation potential exist in the endometrium tissue, can continuously divide and proliferate, and timely supplement the fallen terminally differentiated cells, so that the endometrium can always keep self-renewal and regeneration capacity. In view of this characteristic, the collection of stem cells has been carried out in the prior art using menstrual blood with endometrial tissue discharged during female menstruation as a raw material. At present, in actual operation, collected menstrual blood is mainly filtered to respectively collect the menstrual blood and endometrium, and then the menstrual blood is centrifuged to collect stem cells; digesting the collected endometrial tissue, and collecting stem cells; in the prior art, a separator for separating menstrual blood stem cells is provided, the structure of the separator comprises a plurality of mutually communicated tube bodies, a filter screen is arranged in each tube body, an extractor is arranged at an outlet of the tube body at the bottom, and menstrual blood is sequentially filtered by each layer of tube body through the extraction of the extractor so as to collect stem cells; however, the separator of the above structure has the following problems in practical use: menstrual blood has high viscosity, falling endometrium is carried in the menstrual blood, the menstrual blood coagulates when the environmental temperature is low, a large amount of menstrual blood can be adsorbed by the endometrium, even if an extractor is adopted for extraction, the menstrual blood has poor liquidity and cannot freely pass through each layer of tube body, so that the separation effect of the endometrium and the menstrual blood is poor, the separation of stem cells is influenced, and meanwhile, some white blood cells are also carried in the stem cells separated and collected in the tube body; therefore, in order to solve the above problems, a separator for menstrual blood stem cells has been developed.

SUMMERY OF THE UTILITY MODEL

The utility model discloses the technical problem that will solve is: aiming at the defects of the prior art, the separator for the menstrual blood stem cells is provided, can prevent the menstrual blood from coagulating, promotes the fluidity of the menstrual blood, improves the separation efficiency of the menstrual blood and endometrium, and is more beneficial to the separation and collection of the stem cells.

In order to solve the technical problem, the technical scheme of the utility model is that:

a separator for menstrual blood stem cells comprises a separator body, wherein a detachable sealing cover is arranged at the top of the separator body, a menstrual blood inlet is formed in one side of the top of the sealing cover, a plasma outlet is formed in the bottom of the separator body, and the plasma outlet is communicated with an extractor through a guide pipe; the separator comprises a separator body, a plurality of separation chambers and a plurality of stirring devices, wherein the separation chambers are communicated with one another; and a heating device is arranged outside the separator body.

As an improved technical solution, the heating device is a jacket disposed on the outside of the separator body, and the jacket is provided with a heating medium inlet and a heating medium outlet.

As an improved technical scheme, the heating device is an electric heating jacket arranged on the outside of the separator body, an electric heating wire is arranged in the electric heating jacket, a battery and a switch are arranged on the outside of the electric heating jacket, and the electric heating wire and the switch are respectively and electrically connected with the battery.

As an improved technical scheme, the separation chambers are respectively a first separation chamber, a second separation chamber, a third separation chamber and a fourth separation chamber, wherein the pore diameter of a filter screen in the first separation chamber is 20-30 mu m, the pore diameter of a filter screen in the second separation chamber is 2-8 mu m, and the pore diameter of a filter screen in the third separation chamber is 0.1-0.3 mu m.

As an improved technical scheme, agitating unit includes the puddler and sets up a plurality of auxiliary agitator on the puddler, the one end of puddler pass through the bearing with sealed lid rotates to be connected, the top of puddler is equipped with can dismantle the handle, and is a plurality of auxiliary agitator is including being located first agitator in the first separation chamber and being located second agitator in the second separation chamber, wherein first agitator is the helical blade that the slope set up, the Y shape puddler that the second agitator set up for the slope.

As a further improved technical scheme, a plurality of triangular blades are obliquely arranged on the propeller blade.

As an improved technical scheme, filter screen in the first separating chamber, the filter screen in the second separating chamber and the filter screen in the third separating chamber slope respectively and set up, just filter screen in the first separating chamber the filter screen in the second separating chamber and the filter screen in the third separating chamber is zigzag distribution, one side of first separating chamber, second separating chamber and third separating chamber is equipped with the separator export respectively.

As an improved technical scheme, the sealing cover is in threaded connection with the separator body.

As an improved technical scheme, the bottom of the separator body is provided with supporting legs.

After the technical scheme is adopted, the beneficial effects of the utility model are that:

the separator for the menstrual blood stem cells comprises a separator body, a detachable sealing cover is arranged at the top of the separator body, a menstrual blood inlet is formed in one side of the top of the sealing cover, a plasma outlet is formed in the bottom of the separator body, and the plasma outlet is communicated with an extractor through a guide pipe; the separator comprises a separator body, a plurality of separation chambers, a plurality of sealing covers and a plurality of stirring devices, wherein the separation chambers are communicated with one another; the outside of the separator body is provided with a heating device. The method comprises the following steps that a worker adds collected menstrual blood into a separator body from a menstrual blood inlet on a sterile operating platform, firstly rotates a stirring device for a period of time, stirs the menstrual blood in the separator body through the stirring device to promote blood flow, and part of the menstrual blood slowly and sequentially passes through a plurality of separation chambers under the action of gravity along with the stirring of the stirring device; when the ambient temperature is lower, the separator body is heated by a heating medium in the heating device or the electric heating device, thereby avoiding the menstrual blood from being coagulated when the temperature is lower and promoting the blood to flow; the staff draws the extractor, and menstrual blood passes through a plurality of separating chambers that are linked together each other in proper order under the effect of extractor, and endometrium tissue, stem cell, leucocyte and red blood cell are held back in corresponding separating chamber by the filter screen respectively, and plasma gets into the extractor from the plasma export of separator body. In conclusion, the separator with the structure realizes the separation of endometrial tissue, stem cells, red blood cells and white blood cells in menstrual blood, can promote the fluidity of blood by the stirring device during the separation, can prevent the blood from coagulating by the heating device, prevents the endometrial tissue from adsorbing the blood, greatly improves the separation efficiency, and has simple operation.

Since the heating means is a jacket provided on the outside of the separator body, the jacket is provided with a heating medium inlet and a heating medium outlet. When the ambient temperature is lower, advance the pipe with hot water and communicate the heat medium import, hot water outlet intercommunication heat medium exit tube, advance the pipe through hot water with suitable temperature through the water pump and carry to the jacket inside, utilize the hot water in the jacket to heat the inside menstruation blood of separator body, prevent that menstruation blood from appearing solidifying, promote blood to flow. The heating device adopting the structure has simple structure and is convenient for heating the menstrual blood in the separator body.

Because heating device is the electrical heating cover of setting on the outside of separator body, is equipped with electric heating wire in the electrical heating cover, and the outside of electrical heating cover is equipped with battery and switch, and electric heating wire and switch electricity connect the battery respectively. When the ambient temperature is low, in order to prevent the menstrual blood from coagulating, the switch on the electric heating sleeve is opened, and the menstrual blood in the separator body is heated through the electric heating sleeve. The electric heating sleeve is adopted for heating, the structure is simple, and the use is convenient.

The plurality of separation chambers are respectively a first separation chamber, a second separation chamber, a third separation chamber and a fourth separation chamber, wherein the aperture of a filter screen in the first separation chamber is 20-30 mu m, the aperture of a filter screen in the second separation chamber is 2-8 mu m, and the aperture of a filter screen in the third separation chamber is 0.1-0.3 mu m. Because endometrium tissue, stem cells, white blood cells and red blood cells are different in size, the endometrium tissue can be intercepted in the first separation chamber by adopting filter screens with different apertures, the stem cells are intercepted in the second separation chamber, and the white blood cells and the red blood cells are intercepted in the third separation chamber.

Because agitating unit includes the puddler, sets up a plurality of auxiliary agitator on the puddler, the one end of puddler is passed through the bearing and is connected with sealed lid rotation, and the top of puddler is equipped with the handle, and a plurality of auxiliary agitator are including being located the first agitator of first separation chamber and being located the second agitator of second separation chamber, and wherein first agitator is helical blade, and the Y shape puddler that the second agitator set up for the slope. When the separation operation, the staff holds the handle, then rotates the handle, and the puddler drives first agitator and second agitator and rotates, and first agitator and second agitator stir the menstrual blood in first separating chamber and the second separating chamber, promote blood to flow. The stirring device adopting the structure has reasonable design and simple structure, and improves the fluidity of menstrual blood.

Because the propeller blade is provided with a plurality of triangular blades in an inclined way. The falling endometrial tissue is carried in the menstrual blood, the flow of the menstrual blood can be influenced by the large endometrial tissue, and when the propeller blade rotates along with the stirring shaft, the large endometrial tissue is decomposed into small endometrial tissue by the triangular blade arranged on the propeller blade. The arrangement of the triangular blade can avoid the absorption of menstrual blood by massive inner membrane tissues and the blockage of a filter screen, and further improves the fluidity of blood.

Because the filter screen in the first separating chamber, the filter screen in the second separating chamber and the filter screen in the third separating chamber are respectively arranged in an inclined mode, the filter screen in the first separating chamber, the filter screen in the second separating chamber and the filter screen in the third separating chamber are distributed in a zigzag mode, and the separated matter outlets are respectively arranged on one side of the first separating chamber, one side of the second separating chamber and one side of the third separating chamber. After the separation work is finished, opening a separator outlet at one side of the first separation chamber, a separator outlet of the second separation chamber and a separator outlet of the third separation chamber in sequence, taking out the endometrial tissue trapped by the first separation chamber by using a small spoon made of silica gel or a silica gel stick or tweezers, decomposing the endometrial tissue by using digestive juice, and collecting stem cells; collecting the stem cells intercepted by the second separation chamber by using a small spoon or a silica gel rod made of silica gel; and collecting the white blood cells and the red blood cells intercepted by the third separation chamber by using a small spoon made of silica gel. By adopting the design, the structure is simple, and the separated substances can be conveniently taken out.

Because the bottom of the separator body is provided with the supporting legs. The separator body can be supported and fixed by the supporting legs, so that the separator is convenient to operate.

Drawings

FIG. 1 is a schematic view of a separator for menstrual blood stem cells according to the present invention;

the device comprises a separator body, a plasma outlet, a first separation chamber, a filter screen, a second separation chamber, a filter screen, a third separation chamber, a filter screen, a 131 separator outlet, a fourth separation chamber, a 2-sealing cover, a 20-menstrual blood inlet, a 3-flow guide pipe, a 4-extractor, a 5-stirring rod, a 50-first stirrer, a 500-blade, a 51-second stirrer, a 6-handle, a 7-heating device and 8-supporting legs.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

A separator for menstrual blood stem cells, as shown in figure 1, comprises a separator body 1, a detachable sealing cover 2 is arranged at the top of the separator body 1 (the sealing cover is in threaded connection with the separator body), a menstrual blood inlet 20 is arranged on one side of the top of the sealing cover 2, a plasma outlet 10 is arranged at the bottom of the separator body 1, and the plasma outlet 10 is communicated with an extractor 4 (a disposable injector) through a guide tube 3; the separator body 1 is internally provided with a plurality of mutually communicated separation chambers, the plurality of separation chambers are internally provided with filter screens respectively, wherein the plurality of separation chambers are a first separation chamber 11, a second separation chamber 12, a third separation chamber 13 and a fourth separation chamber 14 respectively (a plasma outlet is positioned at the bottom of the fourth separation chamber), the pore diameter of the filter screen in the first separation chamber 11 is 20-30 mu m, the pore diameter of the filter screen in the second separation chamber 12 is 2-8 mu m, and the pore diameter of the filter screen in the third separation chamber 13 is 0.1-0.3 mu m; the separator comprises a separator body 1, wherein a stirring device penetrating through a plurality of separation chambers and a filter screen is arranged in the separator body 1, the stirring device is rotatably connected with a sealing cover 2, the stirring device comprises a stirring rod 5 and a plurality of auxiliary stirrers arranged on the stirring rod 5, one end of the stirring rod 5 is rotatably connected with the sealing cover 2 through a bearing, a handle 6 is arranged at the top end of the stirring rod 5, the lower end of the stirring rod 5 sequentially penetrates through the centers of the filter screens, the plurality of auxiliary stirrers comprise a first stirrer 50 positioned in a first separation chamber 11 and a second stirrer 51 positioned in a second separation chamber 12, the first stirrer 50 is a helical blade, and the second stirrer 51 is an obliquely arranged Y-shaped stirring rod; the outside of separator body 1 is equipped with heating device 7, and wherein heating device is the jacket (the jacket cover is established at the separator originally) that sets up on the outside of separator body, and the jacket is equipped with heat medium import and heat medium export.

The collected menstrual blood is added into the separator body from a menstrual blood inlet by a worker on a sterile operating platform, the stirring shaft is held by a hand and rotated for a period of time, the first stirrer and the second stirrer are driven to rotate when the stirring shaft rotates, the stirring shaft, the first stirrer (a spiral blade) and the second stirrer (a Y-shaped stirring rod) stir the menstrual blood in the separator body together, the blood flow is promoted, and part of the menstrual blood slowly passes through a plurality of separation chambers in sequence under the action of gravity along with the stirring of the stirring device; when the ambient temperature is lower, advance the pipe with hot water and communicate the heat medium import, hot water outlet intercommunication heat medium exit tube, advance the pipe through hot water with suitable temperature through the water pump and carry to the jacket inside, utilize the hot water in the jacket to heat the inside menstruation blood of separator body, prevent that menstruation blood from appearing solidifying, promote blood to flow. The decimator is twitched again to the staff, menstrual blood passes a plurality of separating chambers that are linked together each other in proper order under the effect of decimator, because the endometrium tissue, the stem cell, leucocyte and erythrocyte size are different, can be detained the endometrium tissue in first separating chamber through the filter screen that adopts different apertures, detain the stem cell in the second separating chamber, detain leucocyte and erythrocyte in the third separating chamber, the plasma that is located the fourth separating chamber gets into the decimator along the honeycomb duct from the plasma export, shift plasma to the collection intraductal through the decimator and preserve. The separator adopting the structure realizes the rapid separation of endometrial tissue, stem cells, white blood cells, red blood cells and plasma in menstrual blood.

Wherein the propeller blade is provided with a plurality of triangular blades 500 on the upper side. The falling endometrial tissue is carried in the menstrual blood, the large endometrial tissue can influence the flow of the menstrual blood, when the screw propeller blade rotates along with the stirring shaft, the large endometrial tissue is decomposed into small tissue by the triangular blade arranged on the screw propeller blade, and the large endometrial tissue can be prevented from adsorbing the menstrual blood and blocking the filter screen by the triangular blade, so that the blood flow is promoted.

Wherein the filter screen in the first separating chamber 11, the filter screen in the second separating chamber 12 and the filter screen in the third separating chamber 13 are respectively arranged in an inclined manner, the filter screen in the first separating chamber, the filter screen in the second separating chamber and the filter screen in the third separating chamber are distributed in a zigzag manner, and the separated substance outlets 131 are respectively arranged on one side of the first separating chamber 11, one side of the second separating chamber 12 and one side of the third separating chamber 13. After the separation work is finished, taking down the jacket, opening a separator outlet at one side of the first separation chamber, a separator outlet of the second separation chamber and a separator outlet of the third separation chamber in sequence, taking out the endometrial tissue intercepted by the first separation chamber by using a small spoon made of silica gel material or a silica gel stick or tweezers, decomposing the endometrial tissue by using digestive juice, centrifuging and collecting stem cells; collecting the stem cells intercepted by the second separation chamber by using a small spoon or a silica gel rod made of silica gel; and collecting the white blood cells and the red blood cells intercepted by the third separation chamber by using a small spoon made of silica gel.

Wherein the width of first separating chamber 11 is greater than the width of second separating chamber 12, and the width of second separating chamber 12 is greater than the width of third separating chamber 13, is equipped with the draw-in groove that is used for the card to fix the filter screen on the inner wall of first separating chamber, second separating chamber and third separating chamber respectively, and the edge both sides of filter screen are equipped with the T fixture block with draw-in groove looks adaptation respectively. By the design, the disassembly and the assembly are convenient.

Wherein the bottom of the separator body 1 is provided with support legs 8. The separator body can be supported and fixed by the supporting legs, so that the separator is convenient to operate.

As another embodiment, the heating device 7 is an electric heating jacket disposed on the outside of the separator body, an electric heating wire is disposed in the electric heating jacket, a battery and a switch are disposed on the outside of the electric heating jacket, and the electric heating wire and the switch are respectively electrically connected to the battery. When the ambient temperature is low, in order to prevent the menstrual blood from coagulating, the switch on the electric heating sleeve is opened, and the menstrual blood in the separator body is heated through the electric heating sleeve. The electric heating sleeve is adopted for heating, the structure is simple, and the use is convenient.

The point that needs to explain is that the puddler is connected for dismantling (the card is solid or threaded connection) with first agitator, when needs wash inside the separator body, according to a direction rotatory sealed lid, and sealed lid is opened the back, holds the handle and shifts out sealed lid and agitating unit and the filter screen in the first separator, then adds the washing liquid and washes the separator is inside. The first stirrer and the second stirrer are detachably connected with the stirring rod, so that the first stirrer and the second stirrer are designed to be conveniently detached and installed.

The above description is only exemplary of the present invention and should not be taken as limiting the scope of the present invention, as any modifications, equivalents, improvements and the like made within the spirit and principles of the present invention are intended to be included within the scope of the present invention.

Claims (9)

1. A separator for menstrual blood stem cells, comprising: the device comprises a separator body, wherein a detachable sealing cover is arranged at the top of the separator body, a menstrual blood inlet is formed in one side of the top of the sealing cover, a plasma outlet is formed in the bottom of the separator body, and the plasma outlet is communicated with an extractor through a guide pipe; the separator comprises a separator body, a plurality of separation chambers and a plurality of stirring devices, wherein the separation chambers are communicated with one another; and a heating device is arranged outside the separator body.

2. A separator for menstrual blood stem cells according to claim 1, wherein: the heating device is a jacket arranged on the outer portion of the separator body, and the jacket is provided with a heating medium inlet and a heating medium outlet.

3. A separator for menstrual blood stem cells according to claim 1, wherein: heating device is for setting up electrical heating cover on the outside of separator body, be equipped with electric heating wire in the electrical heating cover, the outside of electrical heating cover is equipped with battery and switch, electric heating wire and switch electricity connection battery respectively.

4. A separator for menstrual blood stem cells according to claim 1, wherein: the separation chambers are respectively a first separation chamber, a second separation chamber, a third separation chamber and a fourth separation chamber, wherein the pore diameter of a filter screen in the first separation chamber is 20-30 mu m, the pore diameter of a filter screen in the second separation chamber is 2-8 mu m, and the pore diameter of a filter screen in the third separation chamber is 0.1-0.3 mu m.

5. A separator for menstrual blood stem cells according to claim 4, wherein: agitating unit includes puddler and a plurality of auxiliary agitator of setting on the puddler, the one end of puddler pass through the bearing with sealed lid rotates and connects, the top of puddler is equipped with can dismantle the handle, and is a plurality of auxiliary agitator is including being located first agitator in the first separation chamber and being located second agitator in the second separation chamber, wherein first agitator is the helical blade that the slope set up, the Y shape puddler that the second agitator set up for the slope.

6. A separator for menstrual blood stem cells according to claim 5, wherein: the propeller blade is provided with a plurality of triangular blades in an inclined mode.

7. A separator for menstrual blood stem cells according to claim 4, wherein: filter screen in the first separator, the filter screen in the second separator with filter screen in the third separator inclines respectively to set up, just filter screen in the first separator, the filter screen in the second separator and the filter screen in the third separator are zigzag distribution, the second separator one side of first separator, second separator and third separator is equipped with the separator export respectively.

8. A separator for menstrual blood stem cells according to claim 1, wherein: the sealing cover is in threaded connection with the separator body.

9. A separator for menstrual blood stem cells according to claim 1, wherein: the bottom of separator body is equipped with the supporting leg.

Images