CN211412057U - Anti-overflow experimental ware for patch clamp experiments - Google Patents
Anti-overflow experimental ware for patch clamp experiments Download PDFInfo
- Publication number
- CN211412057U CN211412057U CN201921510151.6U CN201921510151U CN211412057U CN 211412057 U CN211412057 U CN 211412057U CN 201921510151 U CN201921510151 U CN 201921510151U CN 211412057 U CN211412057 U CN 211412057U
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- Prior art keywords
- groove
- recording
- liquid
- patch clamp
- tank
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Abstract
The utility model discloses an anti-overflow experimental ware for patch clamp experiments, including the cover glass bottom plate, follow the cover glass bottom plate length is to having set gradually into cistern, recording groove and flowing back groove, it all is linked together through the through-hole between cistern and recording groove, recording groove and the flowing back groove to go into, the bottom of flowing back groove is connected with and passes the siphon drainage tube of cover glass bottom plate. The utility model discloses an anti-overflow experimental ware for patch clamp experiments, its income liquid groove, recording tank, the flowing back groove that utilizes the segmentation intercommunication manage the experimental ware in segments for the environmental stability in the experimental ware influences little, the record of the long-term experiment of being convenient for to the experimental result.
Description
Technical Field
The utility model belongs to the technical field of electrophysiological experiment device, concretely relates to anti-overflow goes out laboratory ware for patch clamp experiments.
Background
The patch clamp is a tool used when ion current of recording through an ion channel reflects the single or multiple molecular activities of the ion channel of a cell membrane, and the recording groove for the patch clamp experiment is a vessel used for preserving brain tissue and culturing cells for a long time and carrying out experiment recording, the current experimental vessel has a recording groove in a cuboid shape, the addition and the discharge of artificial cerebrospinal fluid are mixed together, the stable performance of the artificial cerebrospinal fluid cannot be ensured, and the larger fluctuation of the liquid level can be caused when the liquid is added and discharged, thereby affecting the accuracy of the experiment.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a diaphragm tongs is anti-overflow experimental ware for experiments, its income liquid groove, record tank, the flowing back groove that utilizes the segmentation intercommunication manage the segmentation of experimental ware for the record of experimental ware internal stability, the long-term experiment of being convenient for.
The utility model adopts the technical proposal that: the anti-overflow experimental vessel for the patch clamp experiment comprises a cover glass bottom plate, wherein a liquid inlet groove, a recording groove and a liquid discharge groove are sequentially arranged along the length direction of the cover glass bottom plate, the liquid inlet groove and the recording groove are communicated with each other through holes, and the bottom of the liquid discharge groove is connected with a siphon drainage tube penetrating through the cover glass bottom plate.
Further, along the length direction of the cover glass bottom plate, the length of the recording groove is larger than the length of the liquid inlet groove and the liquid outlet groove.
Furthermore, the liquid inlet groove and the recording groove are communicated with each other through two through holes, and the recording groove and the liquid discharge groove are communicated with each other through two through holes.
Further, the liquid inlet groove is connected with a liquid inlet hose, and the liquid inlet hose is connected with a liquid inlet peristaltic pump.
Further, the liquid discharge groove is connected with a liquid discharge hose, and the liquid discharge hose is connected with a liquid discharge peristaltic pump.
Further, the siphon drainage tube is connected to a waste liquid collecting vessel.
The utility model has the advantages that: the utility model discloses an anti-overflow experimental ware for patch clamp experiments, its income liquid groove, recording tank, the flowing back groove that utilizes the segmentation intercommunication manage the experimental ware in segments for the environmental stability in the experimental ware influences little, the record of the long-term experiment of being convenient for to the experimental result.
Drawings
FIG. 1 is a top view of the anti-overflow experimental vessel for patch clamp experiments;
fig. 2 is a side view of fig. 1.
In the figure, 1, a cover glass bottom plate, 2, a liquid inlet groove, 3, a recording groove, 4, a liquid discharge groove, 5, a liquid inlet hose, 6, a liquid discharge hose and 7, a siphon drainage tube.
Detailed Description
The patch clamp recording tank is an important component of a cell constant temperature recording tank system (a cell recording tank and a constant temperature controller), and is coordinated with a peristaltic pump system to perfuse liquid to a sample, maintain the nutrition of the sample and keep the activity of the sample.
When recording cell channel electrical activity in electrophysiology, patch clamp experiments, it is often necessary to keep cells or tissue sections in a relatively constant temperature system to maintain their activity, which requires that the cell recording tank record the level of the external liquid for a long time to be stable and compatible with a temperature controller and a peristaltic pump matched with the cell recording tank. The utility model discloses the anti-overflow experiment household utensils for the patch clamp experiment can dynamically maintain the stability that the recording fluid is flat to compatible traditional temperature controller and other plug-in components equipment are suitable for in electrophysiology, the patch clamp experiment record multiple cell and brain piece/tissue piece sample, can keep the sample activity more than 10 hours, can realize fast perfusion and dose.
The present invention will be described in detail with reference to the accompanying drawings and specific embodiments.
The utility model provides a structure of anti-overflow experiment household utensils for patch clamp experiments is shown in fig. 1 and fig. 2, including cover glass bottom plate 1, along 1 length of cover glass bottom plate to having set gradually income cistern 2, recording tank 3 and fluid-discharge tank 4, all be linked together through the through-hole between income cistern 2 and recording tank 3, recording tank 3 and the fluid-discharge tank 4, the bottom of fluid-discharge tank 4 is connected with siphon drainage tube 7 that passes cover glass bottom plate 1. The structure in which the liquid inlet tank 2, the recording tank 3, and the liquid discharge tank 4 are separated and communicated with each other contributes to providing a stable environment for the recording tank 3, and the influence of the liquid inlet and the liquid discharge on the recording tank 3 is also small.
Further, the length of the recording tank 3 is longer than the length of the liquid inlet tank 2 and the liquid outlet tank 4 along the length direction of the cover glass bottom plate 1. In this embodiment, the length and the width of the cover glass bottom plate 1 are respectively 50mm and 20mm, the length of the liquid inlet groove 2, the length of the recording groove 3 and the length of the liquid discharge groove 4 are respectively 3mm, 25mm and 3mm along the length direction of the cover glass bottom plate 1, the width of the liquid inlet groove is 10mm, the length of the recording groove 3 is far larger than the length of the liquid inlet groove 2 and the length of the liquid discharge groove 4, so that the environment of the recording groove 3 is stable, the operation space is large, and the experimental observation in a long period of time is guaranteed.
Further, the liquid inlet tank 2 is communicated with the recording tank 3, and the recording tank 3 is communicated with the liquid discharge tank 4 through two through holes. Through increasing through-hole quantity for the liquid level of laboratory vessel is undulant littleer during liquid feeding, flowing back, and internal environment is more stable, thereby also littleer to the influence of experiment.
Furthermore, the liquid inlet groove 2 is connected with a liquid inlet hose 5, and the liquid inlet hose 5 is connected with a liquid inlet peristaltic pump. The liquid discharge groove 4 is connected with a liquid discharge hose 6, and the liquid discharge hose 6 is connected with a liquid discharge peristaltic pump.
Further, the siphon drainage tube 7 is connected to a waste liquid collecting vessel, which is generally placed on a vibration-proof table.
Claims (6)
1. The anti-overflow experimental vessel for the patch clamp experiment is characterized by comprising a cover glass bottom plate, wherein a liquid inlet groove, a recording groove and a liquid discharge groove are sequentially arranged along the length direction of the cover glass bottom plate, the liquid inlet groove and the recording groove are communicated with each other through holes, and the bottom of the liquid discharge groove is connected with a siphon drainage tube penetrating through the cover glass bottom plate.
2. The anti-overflow experimental vessel for patch clamp experiments as claimed in claim 1, wherein the length of the recording groove is longer than the length of the liquid inlet groove and the liquid outlet groove along the length direction of the bottom plate of the cover glass.
3. The anti-overflow experimental vessel for patch clamp experiments as claimed in claim 1, wherein the liquid inlet tank and the recording tank, and the recording tank and the liquid outlet tank are all communicated through two through holes.
4. The anti-overflow experimental vessel for patch clamp experiments as claimed in claim 1, wherein the liquid inlet tank is connected with a liquid inlet hose, and the liquid inlet hose is connected with a liquid inlet peristaltic pump.
5. The anti-overflow experimental vessel for patch clamp experiments as claimed in claim 1, wherein the liquid discharge groove is connected with a liquid discharge hose, and the liquid discharge hose is connected with a liquid discharge peristaltic pump.
6. The anti-overflow labware for patch clamp experiments as claimed in claim 1, wherein the siphon drain is connected to a waste liquid collecting vessel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921510151.6U CN211412057U (en) | 2019-09-11 | 2019-09-11 | Anti-overflow experimental ware for patch clamp experiments |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921510151.6U CN211412057U (en) | 2019-09-11 | 2019-09-11 | Anti-overflow experimental ware for patch clamp experiments |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211412057U true CN211412057U (en) | 2020-09-04 |
Family
ID=72248116
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921510151.6U Expired - Fee Related CN211412057U (en) | 2019-09-11 | 2019-09-11 | Anti-overflow experimental ware for patch clamp experiments |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211412057U (en) |
-
2019
- 2019-09-11 CN CN201921510151.6U patent/CN211412057U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200904 Termination date: 20210911 |