CN211411636U

CN211411636U - Resonant electrophoresis device

Info

Publication number: CN211411636U
Application number: CN201922271947.7U
Authority: CN
Inventors: 李慧; 焦健
Original assignee: Jilin University
Current assignee: Jilin University
Priority date: 2019-12-17
Filing date: 2019-12-17
Publication date: 2020-09-04
Anticipated expiration: 2029-12-17

Abstract

A resonance electrophoresis device relates to the field of molecular biomedicine. The device solves the problem that the existing electrophoresis device is difficult to separate particles with similar mobility in the process of electrophoretic separation, and comprises an electrophoresis tank, an electrode A, an electrode B, an electrode C, an electrode D, an electrode E, an electrode F, an electrode G, an electrode H and a resonant power supply; the inner edge of the electrophoresis tank is circular, and the electrode A, the electrode B, the electrode C, the electrode D, the electrode E, the electrode F, the electrode G and the electrode H are uniformly distributed on the circumference of the inner edge of the electrophoresis tank; the center of the electrophoresis tank is used as the common vertex of the included angle between the electrodes, and the included angle between the adjacent electrodes is 45 degrees; the resonance power supply is provided with eight output ends; the electrode A, the electrode B, the electrode C, the electrode D, the electrode E, the electrode F, the electrode G and the electrode H are respectively and electrically connected with the output end of the resonance power supply; the center of the electrophoresis tank is an electrophoresis area, and the electrophoresis tank is filled with buffer solution; the electrophoretic support is located in an electrophoretic zone.

Description

Resonant electrophoresis device

Technical Field

The utility model relates to a molecular biomedicine field. In particular to a resonance electrophoresis device.

Background

Under the action of an electric field, charged particles move towards an electrode opposite to the electric property of the charged particles, which is called electrophoresis, and in molecular biomedical research and application, an electrophoresis device is often used for separating various particles mixed together, such as biomolecules such as amino acids, peptides, proteins and nucleotides.

Only if the mobility of the particles is different, the particles can be separated by electrophoresis; the particles with small difference of mobility are not easy to separate; the mobility of the particles is related to various factors such as the mass of the particles, the carrying capacity of electricity, the properties of a buffer solution and an electrophoresis support material and the like; the traditional scheme mainly uses a constant electric field for electrophoresis; for some particles with similar electrophoretic mobility under a constant electric field, an electrophoretic separation mode of a reverse pulse or alternating pulse electric field is adopted; because there are an infinite number of electrophoretic particle types, there are always some particles with similar mobility that are difficult to separate under a constant or pulsed electric field.

SUMMERY OF THE UTILITY MODEL

The utility model discloses a solve current electrophoresis apparatus and carry out the electrophoresis separation in-process, be difficult to the problem that realizes the separation to the close particle of mobility, provide a resonance electrophoresis apparatus.

The resonance electrophoresis device comprises an electrophoresis tank, an electrode A, an electrode B, an electrode C, an electrode D, an electrode E, an electrode F, an electrode G, an electrode H and a resonance power supply;

the inner edge of the electrophoresis tank is circular, and the electrode A, the electrode B, the electrode C, the electrode D, the electrode E, the electrode F, the electrode G and the electrode H are uniformly distributed on the circumference of the inner edge of the electrophoresis tank;

the center of the electrophoresis tank is used as a common vertex of an included angle between each two electrodes, and the included angle between every two adjacent electrodes is 45 degrees; the resonance power supply is provided with eight output ends;

the electrode A, the electrode B, the electrode C, the electrode D, the electrode E, the electrode F, the electrode G and the electrode H are respectively and electrically connected with the output end of the resonance power supply;

the center of the electrophoresis tank is an electrophoresis area, and the electrophoresis tank is filled with buffer solution; the electrophoretic support is located in an electrophoretic zone.

The utility model has the advantages that: the utility model provides a device and a method for realizing electrophoretic separation by utilizing different particle Brownian motion characteristic frequencies or different rotation characteristic frequencies. A resonant power supply is used to generate a vibrating and rotating cosine alternating electric field in the electrophoretic region, which affects the mobility when the electrophoretic particles resonate, thereby separating otherwise similar particles.

Drawings

Fig. 1 is a structural diagram of a resonance electrophoresis apparatus according to the present invention.

Detailed Description

First embodiment, the present embodiment is described with reference to fig. 1, and the resonance electrophoresis apparatus includes an electrophoresis tank W, an electrode a, an electrode B, an electrode C, an electrode D, an electrode E, an electrode F, an electrode G, an electrode H, and a resonance power source P.

The inner edge of the electrophoresis tank W is circular, and 8 electrodes, namely an electrode A, an electrode B, an electrode C, an electrode D, an electrode E, an electrode F, an electrode G and an electrode H are fixedly arranged at the inner edge; all the electrodes are uniformly distributed on the circumference where the inner edge of the electrophoresis tank W is located, the circle center O of the electrophoresis tank W is used as the common vertex of the included angle between the electrodes, and the included angle between the adjacent electrodes is 45 degrees; the resonant power supply P has 8 output ends, OA, OB, OC, OD, OE, OF, OG and OH; the electrode A is electrically connected with the OA output end of the resonant power supply P;

the electrode B is electrically connected with an OB output end of the resonant power supply P; the electrode C is electrically connected with an OC output end of the resonant power supply P; the electrode D is electrically connected with the OD output end of the resonant power supply P; the electrode E is electrically connected with the OE output end of the resonant power supply P; the electrode F is electrically connected with the OF output end OF the resonance power supply P; the electrode G is electrically connected with the OG output end of the resonant power supply P; the electrode H is electrically connected with the OH output end of the resonance power supply P;

a square electrophoresis area U is arranged in the center of the electrophoresis tank W, and four vertexes of the square where the electrophoresis area U is located are I, J, K and L respectively; the diagonal line IL of the square where the electrophoresis area U is located is superposed with the connecting line of the electrode HD, and the diagonal line JK is superposed with the connecting line of the electrode BF; the electrophoresis tank W is filled with buffer solution; the electrophoresis support is located in electrophoresis zone U.

In this embodiment, the output voltages of the 4 output terminals OA, OE, OC and OG of the resonant power supply are sine wave ac; if the angular frequency is defined as omega/2, t is time, the maximum value is defined as U_MThen;

the voltage VA function expression on the electrode A is shown as follows;

VA＝U_Mcos(ωt/2)

the voltage VE function expression on the electrode E is as follows;

VE＝-U_Mcos(ωt/2)

the voltage VC function expression on the electrode C is as follows;

VC＝U_Mcos(ωt+π/2)

the function expression of the voltage VG on the electrode G is shown as follows;

VG＝-U_Mcos(ωt+π/2)

the other 4 output ends OB, OF, OD and OH OF the resonant power supply are direct current; the voltage on the electrode B is;

VB＝U_M

the voltage on the electrode F is;

VF＝-U_M

the voltage on the electrode D is;

VD＝-U_M

the voltage on the electrode H is;

VH＝U_M

establishing a plane rectangular coordinate system by taking a point O at the center of an electrophoresis area U as a coordinate origin, taking a connecting line of an electrode C and an electrode G as an x axis, and taking a connecting line of an electrode E and an electrode A as a y axis; the electrophoretic region is near the center;

the electric field intensity in the y-axis direction is;

Uy＝U_M[1+cos(ωt/2)]

the electric field strength in the x-axis direction is:

Ux＝U_Mcos(ωt+π/2)

in the resonance electrophoresis apparatus according to the present embodiment, the particles subjected to electrophoresis in the electric field are subjected to an electric field force in both the x-axis direction and the y-axis direction, and move in the electrophoresis region in a meandering manner along a serpentine curve as the electric field direction rotates; the electric field force in the x-axis direction and the y-axis direction both accord with simple harmonic vibration; the stress direction of the y axis is always kept unchanged, the magnitude of the stress direction is changed according to the cosine law, and the angular frequency is omega/2; the stress direction of the x axis can be changed in two directions, the magnitude of the stress changes according to the cosine law, and the angular frequency is omega; the rotation and swing in the direction of the electric field also accord with simple harmonic vibration, and the angular frequency is omega/2; during the positive half cycle of the VC alternating-current voltage, the electric field rotates in the anticlockwise direction; during the negative half cycle of the VC alternating-current voltage, the electric field rotates clockwise; an offset electric field exists in the y-axis direction of the electrophoresis area, the integral of the electric field force is not zero, and the integral of the electric field force in the x-axis direction of the electrophoresis area is zero, so that the electrophoresis particles finally move only along the y-axis direction;

in the case of the electrophoretic device according to this embodiment, when no power is applied, the electrophoretic particles are bound by the electrophoretic support and cannot move freely without being separated from the bound region; meanwhile, the particles are also subjected to the random impact of molecules in the buffer solution, so that only vibration in a bound area can be realized; the frequency of vibration is referred to as the brownian motion characteristic frequency of such electrophoretic particles; controlling the output angular frequency of the resonant power supply so that when ω equals the brownian motion characteristic frequency of an electrophoretic particle, the electrophoretic particle will resonate and the amplitude of the resonant electrophoretic particle oscillation will be significantly increased so that it can move from one location to another without being bound by the electrophoretic support; under the resonance condition, the longitudinal offset electric field makes the electrophoretic particles easier to move, and the mobility is obviously increased; the vibration amplitude and the mobility of other electrophoresis particles which do not generate resonance are unchanged;

in this embodiment, the electrophoretic particles are drawn by the rotating electric field to rotationally oscillate; different types of electrophoretic particles have different charge distributions, so that the moments subjected to electric field force are different; the different types of electrophoretic particles have different moments of inertia, so the frequency of resonance with the rotating electric field is different; the resonant frequency is referred to as the characteristic frequency of rotation of the electrophoretic particle; controlling the output angular frequency of the resonant power supply to enable the electrophoretic particles to generate resonant oscillation when omega is equal to the rotation characteristic frequency of certain electrophoretic particles, and enabling the electrophoretic particles to rotate and oscillate in a larger amplitude, so that the electrophoretic particles are easier to separate from the constraint of an electrophoretic support, and the mobility is increased; and the other electrophoretic particles which do not generate resonance have small rotation swing amplitude, and the change of the mobility is negligible.

Claims

1. The resonance electrophoresis device comprises an electrophoresis tank (W), an electrode A, an electrode B, an electrode C, an electrode D, an electrode E, an electrode F, an electrode G, an electrode H and a resonance power supply (P); the method is characterized in that:

the inner edge of the electrophoresis tank (W) is circular, and the electrode A, the electrode B, the electrode C, the electrode D, the electrode E, the electrode F, the electrode G and the electrode H are uniformly distributed on the circumference of the inner edge of the electrophoresis tank (W);

the center of the electrophoresis tank (W) is used as the common vertex of the included angle between the electrodes, and the included angle between the adjacent electrodes is 45 degrees; the resonance power supply (P) is provided with eight output ends;

the electrode A, the electrode B, the electrode C, the electrode D, the electrode E, the electrode F, the electrode G and the electrode H are respectively and electrically connected with the output end of the resonance power supply (P);

an electrophoresis area (U) is arranged at the center of the electrophoresis tank (W), and a buffer solution is filled in the electrophoresis tank (W); the electrophoretic support is located in the electrophoretic zone (U).

2. A resonant electrophoretic device, according to claim 1, wherein: and a square electrophoresis area (U) is arranged at the center of the electrophoresis tank (W), two diagonal lines of the electrophoresis area (U) are respectively superposed with the connecting line of the electrode H and the electrode D, and the connecting line of the electrode B and the electrode F.

3. A resonant electrophoretic device, according to claim 1, wherein:

the output voltages of four output ends OA, OE, OC and OG of the resonant power supply (P) are all alternating-current sine waves; defining the angular frequency of the AC sine wave as omega/2 and the maximum value as U_MAnd then:

the voltages on electrode a, electrode E, electrode C and electrode G are: u shape_Mcos (ω t + π/2), t is time;

the output voltages OF four output ends OB, OF, OD and OH OF the resonance power supply (P) are all direct current, and the voltages on the electrode B and the electrode H are all U_MThe voltage on electrode F and electrode D are both-U_M。

4. A resonant electrophoretic device, according to claim 1, wherein: establishing a plane rectangular coordinate system by taking a central point O of an electrophoresis area (U) as a coordinate origin, taking a connecting line of an electrode C and an electrode G as an x axis, and taking a connecting line of an electrode E and an electrode A as a y axis; the electric field intensity in the center of the electrophoretic region, i.e. the y-axis direction and the x-axis direction, is expressed by the following formula:

Uy＝U_M[1+cos(ωt/2)]

Ux＝U_Mcos(ωt+π/2)。