CN211379324U - Manual feeding observation cup for diaphorina citri - Google Patents
Manual feeding observation cup for diaphorina citri Download PDFInfo
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- CN211379324U CN211379324U CN201922408622.9U CN201922408622U CN211379324U CN 211379324 U CN211379324 U CN 211379324U CN 201922408622 U CN201922408622 U CN 201922408622U CN 211379324 U CN211379324 U CN 211379324U
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- cup
- feeding
- film
- diaphorina citri
- cup body
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Abstract
The utility model relates to a manual feeding observation cup for diaphorina citri, which comprises a feeding liquid layer, a cup body and a cup bottom which are arranged from top to bottom in sequence; the feeding liquid layer comprises an upper film, a lower film and feeding liquid between the upper film and the lower film; the cup body is a transparent cylinder I with openings at two ends; the cup bottom is a transparent cylinder II with an opening at the upper end and a sealing bottom cover at the lower end, the cup body can be inserted into the cup bottom, the height of the cup bottom is smaller than that of the cup body, a breathable screen window is arranged at the center of the bottom cover, and a plurality of supporting suckers are uniformly distributed on the lower surface of the bottom cover on the outer side of the breathable screen window; raise the upper end opening of liquid layer and cup and can dismantle sealing connection, in the lower extreme of cup inserted the bottom of a cup, the bottom of a cup upper end is sealed through sealing membrane and cup outer wall can dismantle. The utility model has simple structure, convenient operation and fast assembly; easy to observe and wash; host plants are not needed, and artificial feeding can be realized; the feeding liquid can be replaced or added at any time; can be used for rapid pesticide bioassay experiments, RNA interference or insect-resistant protein evaluation and the like.
Description
Technical Field
The utility model relates to a technical field is raised to the diaphorina citri, specifically relates to an artifical observation cup of feeding of diaphorina citri.
Background
Asian Citrus psyllid (Diaphorina citri) is a main disease transmission vector insect which transmits Citrus Huanglongbing (HLB), has piercing-sucking mouthparts and can suck phloem juice of rutaceae plants, the pathogen of the Citrus Huanglongbing in China is Asian species of phlobacterium (Candidatus Liberibacter asiaticus', CLas), the bacterium can proliferate in psyllids, and the psyllids can inject bacterium-carrying saliva into host plants to cause the diffusion and spread of the Citrus Huanglongbing, thereby seriously threatening the development of the Citrus industry.
In recent years, various pest control technologies are emerging continuously, reasonable and effective biological measurement methods are needed for evaluating the control effect, and currently, in-vitro branch tip feeding methods and artificial feed feeding methods are available for Asian diaphorina citri. The in vitro shoot feeding method is to directly put the citrus or murraya paniculata shoot which is favorite by the psyllids into a centrifugal tube for the psyllids to take. The method has the advantages of convenience and rapidness, and simulates the natural environment for feeding insects. The method has the defects that the cut at the tail end of the tender tip of the citrus needs to be soaked in a to-be-detected medicament (or dsRNA or insecticidal protein) in advance for absorption for a period of time before the cut can be used for feeding the psyllids of the citrus, the tail end of a branch needs to be soaked in the to-be-detected medicament all the time in the experimental process, but the medicament is not uniformly diffused in the branch, the concentration is not uniform, the result fluctuation is large, more biological repetition is needed, the tender tip needs to be replaced frequently, and the observation is. The artificial feed feeding method is to feed the psyllids for a short time by preparing an artificial feeding solution for the psyllids or using a 30% sucrose solution and the like. The method can mix the to-be-detected medicament (or dsRNA or insecticidal protein) into the feed for direct feeding, and the psyllids with uniform concentration of the to-be-detected medicament can directly feed without pretreatment, so that the repeatability of the experimental result is good. The defects that the conventional artificial feed for the diaphorina citri is not perfect enough and has few varieties, and the whole life history of the diaphorina citri cannot be fed. The artificial feed is flowing liquid, has certain viscosity, is not easy to feed, and is easy to die once wings of the psyllids are stuck. Therefore, an effective and easily-observed device is needed, and the artificial feeding liquid of the mixed medicament (or small molecules) is directly sent into the diaphorina citri body through a mouth needle to perform bioassay evaluation by means of the mode that the diaphorina citri takes food autonomously.
SUMMERY OF THE UTILITY MODEL
Aiming at the defects in the prior art, the utility model provides a manual feeding observation cup for diaphorina citri, which comprises a feeding liquid layer, a cup body and a cup bottom which are arranged from top to bottom in sequence; the feeding liquid layer comprises an upper film, a lower film and feeding liquid between the upper film and the lower film; the cup body is a transparent cylinder I with openings at two ends; the cup bottom is a transparent cylinder II with an opening at the upper end and a sealing bottom cover at the lower end, the inner diameter of the cup bottom is matched with the outer diameter of the cup body, so that the cup body can be inserted into the cup bottom, the height of the cup bottom is smaller than that of the cup body, a breathable screen window is arranged at the center of the bottom cover, and a plurality of supporting suckers are uniformly distributed on the lower surface of the bottom cover on the outer side of the breathable screen window along the circumferential direction;
raise the upper end opening of liquid layer and cup and can dismantle sealing connection, in the lower extreme of cup inserted the bottom of cup, the bottom of cup upper end is through sealing membrane and cup outer wall can dismantle sealedly.
The utility model discloses a detachable of each other raises the liquid layer, cup and bottom of cup mutually support, adopt double-deck film feeding method, will raise the liquid seal and form double-deck liquid sandwich sealing layer of raising in the double-deck film that upper and lower film is constituteed to supply oranges and tangerines psylla mouth needle to pierce through the film and get the food, need not often to open and feed the observation cup, improved the leakproofness, can dismantle the connection through cup and bottom of cup, make the bottom of cup can change the washing at any time, in order to avoid raising the in-process and accumulate too much oranges and tangerines psylla excrement. Be equipped with ventilative screen window on the bottom through the cup bottom, can ventilate and breathe freely, can prevent that oranges and tangerines psylla adult or nymph from escaping again, be equipped with the support sucking disc at the bottom lower surface, can fix and feed and observe the cup, and can raise and feed and observe the bottom of the cup in order to keep the circulation of air.
Preferably, the upper film, the lower film and the sealing film are Parafilm films, so that the method is convenient and safe, and cannot generate adverse effects on feeding of the diaphorina citri.
Preferably, the mesh number of the ventilation screen window is 120 meshes, so that diaphorina citri is prevented from escaping from the ventilation screen window while air permeability is ensured.
Preferably, the supporting sucker is a transparent silica gel pad, the feeding observation cup can be stably fixed after being assembled due to the arrangement of the supporting sucker, and the supporting sucker has a certain height, so that the good air permeability of the air-permeable screen window can be ensured, and the feeding observation cup can be smoothly exchanged with the outside air.
Preferably, the cup body is a cylinder supported by quartz glass.
Preferably, the cup bottom is a cylinder made of polystyrene plastic.
The utility model discloses still including enabling other subassemblies of the artifical observation cup normal use of feeding of a oranges and tangerines psylla, like the insect-attracting pipe and the sealing film etc. that will get the put psylla, the sealing film is the Parafilm, directly glues on glass, can not drop, is the conventional technological means in this field. In addition, the device or the component which is not limited in the utility model adopts the conventional technical means in the field, such as the supporting sucker, the sealing film, the transparent screen window and the like, which are common equipment in the field.
Form two membrane feeding liquid sandwich sealing layers in the double-layer film to supply the diaphorina citri kou needle to pierce through the film and get the food, need not often to open and feed and observe the cup, improved the leakproofness, can dismantle the connection through cup and bottom of cup, make the bottom of cup change the washing at any time, in order to avoid raising the in-process and accumulate too much diaphorina citri excrement. Be equipped with ventilative screen window on the bottom through the cup bottom, can ventilate and breathe freely, can prevent that oranges and tangerines psylla adult or nymph from escaping again, be equipped with the support sucking disc at the bottom lower surface, can fix and feed and observe the cup, and can raise and feed and observe the bottom of the cup in order to keep the circulation of air. Simple structure, convenient operation, easily observation adopt double-deck film feeding method, only need seal the liquid of raising in double-deck film, need not often to open the observation cup of feeding. When the diaphorina citri is fed and observed and a biological test experiment is carried out, the diaphorina citri can be conveniently detached, replaced and cleaned, and the observation is more simple, convenient and visual.
The utility model has the advantages of simple structure, convenient operation and fast assembly; easy to observe and wash; host plants are not needed, and artificial feeding can be realized; the feeding liquid can be replaced or added at any time; can be used for rapid pesticide bioassay experiments, RNA interference or insect-resistant protein evaluation and the like.
Drawings
The present invention will be further explained with reference to the drawings and examples.
Fig. 1 is a schematic structural view of a cup body and a cup bottom in embodiment 1 of the present invention;
fig. 2 is a schematic structural view of a cup body and a cup bottom in embodiment 2 of the present invention;
FIG. 3 is a schematic view of the assembled structure of the feeding cup of the utility model;
fig. 4 is a comparison graph of psyllid midgut before and after the citrus psyllid is fed by the feeding cup of the present invention.
In the figure: 1. the feed comprises a cup body, 2 parts of a cup bottom, 3 parts of a breathable screen window, 4 parts of a supporting sucker, 5 parts of a lower film, 6 parts of a diaphorina citri, 7 parts of a feeding liquid, 8 parts of a sealing film and 9 parts of an upper film.
Detailed Description
The present invention will be described more clearly with reference to the accompanying drawings and specific embodiments in the embodiments of the present invention, and the description herein is only for explaining the present invention, but not for limiting the present invention. Based on the embodiments of the present invention, any modifications, equivalent replacements, improvements, etc. made by other embodiments obtained by those skilled in the art without creative efforts should be included in the protection scope of the present invention.
Examples
The present invention will be described more clearly with reference to the accompanying drawings and specific embodiments in the embodiments of the present invention, and the description herein is only for explaining the present invention, but not for limiting the present invention. Based on the embodiments of the present invention, any modifications, equivalent replacements, improvements, etc. made by other embodiments obtained by those skilled in the art without creative efforts should be included in the protection scope of the present invention.
Example 1:
as shown in fig. 1, the utility model provides a manual feeding observation cup for diaphorina citri, which comprises a feeding liquid layer, a cup body 1 and a cup bottom 2 which are arranged from top to bottom in sequence; the feeding liquid layer comprises an upper film 9, a lower film 5 and a feeding liquid 7 between the upper film and the lower film; the cup body 1 is a transparent cylinder I with openings at two ends; the cup bottom 2 is a transparent cylinder II with an opening at the upper end and a sealing bottom cover at the lower end, the inner diameter of the cup bottom 2 is matched with the outer diameter of the cup body 1, so that the cup body 1 can be inserted into the cup bottom 2, the height of the cup bottom 2 is smaller than that of the cup body 1, a breathable screen window 3 is arranged in the center of the bottom cover, and a plurality of supporting suckers 4 are uniformly distributed on the lower surface of the bottom cover on the outer side of the breathable screen window 3 along the circumferential direction;
raise the upper end opening of liquid layer and cup 1 and can dismantle sealing connection, in the lower extreme of cup 1 inserted the bottom of cup 2, 2 upper ends of the bottom of cup and through sealing membrane 8 and cup 1 outer wall can dismantle sealedly.
The feeding cup body 1 is designed into a cylindrical shape, two ends of the feeding cup body are open, the feeding cup body is made of quartz glass with the thickness of 2mm, the inner diameter of the feeding cup body is 2.9cm, and the height of the feeding cup body is 6 cm; the cup bottom 2 is designed to be cylindrical, the upper end of the cup bottom is provided with an opening, the inner diameter of the opening at the upper end is 3.5cm and the inner diameter of the air-permeable screen window 3 is 2cm and the height is 1.2 cm.
The upper film, the lower film and the sealing film are made of Parafilm, so that the method is convenient and safe, and cannot generate adverse effect on feeding of the diaphorina citri.
The mesh number of the breathable screen window is 120 meshes, so that the diaphorina citri is prevented from escaping from the breathable screen window while the breathability is ensured.
The supporting suction cups 4 are transparent silica gel pads, the number of the supporting suction cups is not less than 3, and the single size of the supporting suction cups is 9mm multiplied by 3 mm. The feeding observation cup can be stably fixed after being assembled by arranging the suction cup support, the support suction cup 4 has a certain height, and good air permeability of the air permeable screen window 3 can be ensured, so that the feeding observation cup can be smoothly exchanged with outside air.
The cup body 1 is a cylinder supported by quartz glass.
The cup bottom 2 is a cylinder made of polystyrene plastic.
Example 2:
as shown in fig. 2, the utility model provides a manual feeding observation cup for diaphorina citri, which comprises a feeding liquid layer, a cup body 1 and a cup bottom 2 which are arranged from top to bottom in sequence; the feeding liquid layer comprises an upper film 9, a lower film 5 and a feeding liquid 7 between the upper film and the lower film; the cup body 1 is a transparent cylinder I with openings at two ends; the cup bottom 2 is a transparent cylinder II with an opening at the upper end and a sealing bottom cover at the lower end, the inner diameter of the cup bottom 2 is matched with the outer diameter of the cup body 1, so that the cup body 1 can be inserted into the cup bottom 2, the height of the cup bottom 2 is smaller than that of the cup body 1, a breathable screen window 3 is arranged in the center of the bottom cover, and a plurality of supporting suckers 4 are uniformly distributed on the lower surface of the bottom cover on the outer side of the breathable screen window 3 along the circumferential direction;
raise the upper end opening of liquid layer and cup 1 and can dismantle sealing connection, in the lower extreme of cup 1 inserted the bottom of cup 2, 2 upper ends of the bottom of cup and through sealing membrane 8 and cup 1 outer wall can dismantle sealedly.
The cup body of the feeding cup is designed to be cylindrical, two ends of the cup body are open, the cup body is made of quartz glass with the thickness of 2mm, and the inner diameter of the cup body is 6cm and the height of the cup body is 6 cm. The cup bottom is designed to be cylindrical, the upper end of the cup bottom is provided with an opening, the inner diameter of the upper end opening is 6.6cm and is made of polystyrene plastics, and the inner diameter of the breathable screen window is 3cm and the height of the breathable screen window is 1.4 cm.
The upper film, the lower film and the sealing film are made of Parafilm, so that the method is convenient and safe, and cannot generate adverse effect on feeding of the diaphorina citri.
The mesh number of the breathable screen window is 120 meshes, so that the diaphorina citri is prevented from escaping from the breathable screen window while the breathability is ensured.
The supporting suction cups are transparent silica gel pads, the number of the supporting suction cups is not less than 3, and the single size of the supporting suction cups is 9mm multiplied by 3 mm. The feeding observation cup can be stably fixed after being assembled by arranging the suction cup, and the supporting suction cup has a certain height, so that the good air permeability of the air-permeable screen window can be ensured, and the feeding observation cup can be smoothly exchanged with outside air.
The cup body is a cylinder supported by quartz glass.
The cup bottom is a cylinder made of polystyrene plastic.
When the special Parafilm is used, the upper end opening of the cup body 1 is sealed by a Parafilm film, the prepared feeding liquid 7 is gently dripped onto the sealed Parafilm by a pipette, and then a layer of Parafilm is sealed on the feeding liquid 7, so that the feeding liquid 7 is uniformly distributed between the two layers of Parafilm. Fumigating diaphorina citri with carbon dioxide, transferring to the cup bottom 2, or quickly transferring diaphorina citri to the cup body 1 through an insect suction pipe, quickly closing the cup body 1 and the cup bottom 2, and fixing the cup body 1 and the cup bottom 2 by using a Parafilm. During the feeding period, the diaphorina citri feeding liquid 7 or the cup bottom 2 can be replaced according to the requirement to clean diaphorina citri excrement. As shown in figure 3, the adult diaphorina citri is automatically clustered on the feeding liquid layer at the top of the cup body 1, feeding is carried out on the feeding liquid 7 in the sandwich of the two layers of films, and in figure 4, the midgut of the diaphorina citri before (B) and after (A) is fed can show that the diaphorina citri can be fed in the device independently (the part enclosed by the oval in A in figure 4 shows the artificial feeding liquid which is fed).
The foregoing description of the embodiments of the invention has been presented for purposes of illustration and not limitation, and is not intended to be exhaustive or limited to the embodiments disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the illustrated embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (6)
1. The utility model provides a manual feeding observation cup of diaphorina citri which characterized in that: comprises a feeding liquid layer, a cup body and a cup bottom which are arranged from top to bottom in sequence; the feeding liquid layer comprises an upper film, a lower film and feeding liquid between the upper film and the lower film; the cup body is a transparent cylinder I with openings at two ends; the cup bottom is a transparent cylinder II with an opening at the upper end and a sealing bottom cover at the lower end, the inner diameter of the cup bottom is matched with the outer diameter of the cup body, so that the cup body can be inserted into the cup bottom, the height of the cup bottom is smaller than that of the cup body, a breathable screen window is arranged at the center of the bottom cover, and a plurality of supporting suckers are uniformly distributed on the lower surface of the bottom cover on the outer side of the breathable screen window along the circumferential direction;
raise the upper end opening of liquid layer and cup and can dismantle sealing connection, in the lower extreme of cup inserted the bottom of cup, the bottom of cup upper end is through sealing membrane and cup outer wall can dismantle sealedly.
2. The artificial feeding observation cup for diaphorina citri according to claim 1, which is characterized in that: the upper film, the lower film and the sealing film are Parafilm films.
3. The artificial feeding observation cup for diaphorina citri according to claim 1, which is characterized in that: the mesh number of the breathable screen window is 120 meshes.
4. The artificial feeding observation cup for diaphorina citri according to claim 1, which is characterized in that: the supporting sucker is a transparent silica gel pad.
5. The artificial feeding observation cup for diaphorina citri according to claim 1, which is characterized in that: the cup body is a cylinder supported by quartz glass.
6. The artificial feeding observation cup for diaphorina citri according to claim 1, which is characterized in that: the cup bottom is a cylinder made of polystyrene plastic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922408622.9U CN211379324U (en) | 2019-12-28 | 2019-12-28 | Manual feeding observation cup for diaphorina citri |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922408622.9U CN211379324U (en) | 2019-12-28 | 2019-12-28 | Manual feeding observation cup for diaphorina citri |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211379324U true CN211379324U (en) | 2020-09-01 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201922408622.9U Expired - Fee Related CN211379324U (en) | 2019-12-28 | 2019-12-28 | Manual feeding observation cup for diaphorina citri |
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| Country | Link |
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| CN (1) | CN211379324U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113575518A (en) * | 2021-07-27 | 2021-11-02 | 四川省农业科学院植物保护研究所 | Method for insect film toxicant feeding and application thereof |
-
2019
- 2019-12-28 CN CN201922408622.9U patent/CN211379324U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113575518A (en) * | 2021-07-27 | 2021-11-02 | 四川省农业科学院植物保护研究所 | Method for insect film toxicant feeding and application thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200901 Termination date: 20211228 |