CN211348255U - Fluorescence immunoassay appearance - Google Patents

Fluorescence immunoassay appearance Download PDF

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Publication number
CN211348255U
CN211348255U CN201921729645.3U CN201921729645U CN211348255U CN 211348255 U CN211348255 U CN 211348255U CN 201921729645 U CN201921729645 U CN 201921729645U CN 211348255 U CN211348255 U CN 211348255U
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sample
incubation
driving mechanism
card
area
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CN201921729645.3U
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招睿雄
杜沛深
蒋庭彦
肖林
甘剑亮
卢鹏
聂浩英
高峰
章国建
张二盈
李镇雄
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Shenzhen Kingfocus Biomedical Engineering Co ltd
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Shenzhen Kingfocus Biomedical Engineering Co ltd
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Abstract

The utility model relates to a fluorescence immunoassay analyzer, which comprises an underframe, a sampling quantitative mechanism, a clamping and supporting mechanism, an incubation detection mechanism, a humidity control mechanism and a sample introduction mechanism; the sampling quantitative mechanism is arranged at the rear side of the incubation detection mechanism, the sample injection mechanism is arranged at one side of the incubation detection mechanism, the humidity control mechanism is arranged between the incubation detection mechanism and the sample injection mechanism, and the clamping and supporting mechanism is arranged at the upper end of the incubation detection mechanism; the sampling quantitative mechanism comprises a mounting frame connected with the underframe, a mechanical arm capable of realizing three-axis transmission is arranged on the mounting frame, and a quantitative pump, a bar code scanner and an optical measurer are arranged on the mechanical arm. The utility model discloses the test item can make up at random, can satisfy the big condition of disposable sample size and also can adapt to the sample and be little and frequent condition, and strong adaptability uses the scene nimble, has still reduced design cost and manufacturing cost, adopts the wet mechanism of accuse, realizes the test card and hatches and the constant humidity of testing process, has improved measuring stability and accuracy.

Description

Fluorescence immunoassay appearance
Technical Field
The utility model relates to an immunoassay appearance technical field, more specifically say and indicate a fluorescence immunoassay appearance.
Background
The existing full-automatic dry type fluorescence immunoassay analyzer generally has the following characteristics: the reagent card adopts a card bin type structure, the reagent card is pre-loaded into the cartridge clip, the cartridge clip package is torn off and loaded into the card bin when in use, and the equipment automatically takes out the reagent card from the card bin for testing; the reagent card incubation module adopts a rotating disc type structure or an orbital production line type structure, and test cards of equipment with the rotating disc type structure are radially distributed around an incubation disc; the track assembly line type structure test cards are connected front and back along the assembly line track and are arranged one by one; there are generally two ways of sample aspiration: a sampling needle or Tip; the sampling needle mode can puncture a whole blood sample, and can carry out uniform mixing operation through a mechanism, but a liquid path system is needed to clean the sampling needle; the Tip head mode generally needs to open a cover for sample loading, the whole blood sample generally cannot be mixed uniformly, the Tip head is needed as a consumable material, and the problem of cross contamination does not exist; as the sample container, a whole blood collection tube, a urine test tube, a micro blood collection tube, or the like is generally used; the sample feeding mode generally adopts a sample rack, the sample rack generally feeds samples from right to left or from front to back, and the sample rack enters the device and exits from another outlet or returns from the original path after sampling.
However, the existing products generally have the following problems: 1. the cartridge type structure generally has the following problems: 1) the development difficulty is high, the reliability is poor, and the problem of shell clamping is easy to occur; 2) the number of the card bins is limited, the number of the common card bin type equipment capable of loading the cartridge clips at the same time is 2-4, so that the number of the items capable of being detected at the same time is limited, if the number of the card bins is increased, the volume of the equipment needs to be correspondingly and obviously increased, and meanwhile, due to the structural characteristics of the card bins, the number of the cartridge clips is hardly increased greatly; 3) because the number of reagent cards which can be loaded in the card bin at one time is large, if the test process cannot be used up, the rest test cards also need to be stored under the conditions of temperature control and humidity control, extra burden is added to the design of equipment, the stability is influenced, and if the test cards cannot be used up in the valid period, the test cards need to be scrapped, so that waste is caused; 4) the cartridge type structure requires certain requirements for the structure of the test card, and in order to reliably take the card from the cartridge holder, the test card generally needs to be specially customized, so that the development cost is increased; 2. the rotating disc type incubation module has the advantages that the test cards are radially arranged, the circumference of the incubation module is correspondingly increased when one test card is added, if the number of the test cards is large, the diameter of the incubation disc needs to be increased, the volume of equipment also becomes very large, and meanwhile, because the rotating disc type structure needs to be specially manufactured, if the equipment needs to be upgraded and the number of the test cards is increased, the rotating disc needs to be manufactured again, the whole structure, devices and the volume of the equipment need to be redesigned, and the equipment is not beneficial to upgrading; the track assembly line type structure needs the test cards to be arranged in a row to pass through the track for measurement, but because the length of the track is limited, the number of the test cards which are simultaneously incubated on the machine each time is limited, and the test efficiency is not high; 3. at present, most of automatic equipment does not have a temperature control incubation function, and influences the stability of a measuring result of a test card; 4. many equipment use the steel needle to sample at present, because the steel needle is used repeatedly, all need wash after having adopted the sample at every turn, avoid cross contamination, consequently equipment need design corresponding washing liquid way system and be equipped with outside washing liquid and waste liquid bucket, lead to the volume of equipment to increase, and the reliability reduces, simultaneously because the introduction of liquid way has increased the design degree of difficulty and the risk that the liquid way was revealed. In addition, the cleaning of the sample needle by the cleaning mode has the residual risk and influences the reliability of the test, and in addition, the scrap of the rubber plug of the blood sampling tube can be brought in the puncture process of the steel needle, so that the problems of liquid path blockage and the like are caused; 5. the device for sampling by using the Tip head is incapable of shaking the blood sampling tube by using a shaking mechanism like a steel needle scheme because a whole blood sample needs to be opened and put on a machine, the device of the type usually requires an operator to manually shake the sample and then put on the machine for measurement, and under the condition of more samples, the former sample is still tested, and the latter sample is precipitated and layered due to overlong waiting time, so that the accuracy of the test is seriously influenced; 6. most of fluorescence immunoassay analyzers in the market do not have the humidity control function at present, and the measurement stability and accuracy of the test card are influenced by other influences brought by the environment, so that the final test result is influenced.
SUMMERY OF THE UTILITY MODEL
The utility model aims to overcome the defects of the prior art and provide a fluorescence immunoassay analyzer.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
a fluorescence immunoassay analyzer comprises a bottom frame, a sampling quantitative mechanism, a clamping and supporting mechanism, an incubation detection mechanism, a humidity control mechanism and a sample introduction mechanism, wherein the sampling quantitative mechanism, the clamping and supporting mechanism, the incubation detection mechanism, the humidity control mechanism and the sample introduction mechanism are arranged on the bottom frame; the sampling quantitative mechanism is arranged at the rear side of the incubation detection mechanism, the sample injection mechanism is arranged at one side of the incubation detection mechanism, the humidity control mechanism is arranged between the incubation detection mechanism and the sample injection mechanism, and the clamping and supporting mechanism is arranged at the upper end of the incubation detection mechanism; the sampling quantitative mechanism comprises a mounting frame connected with the underframe, a mechanical arm capable of realizing three-axis transmission is arranged on the mounting frame, and a quantitative pump, a bar code scanner and an optical measurer are arranged on the mechanical arm.
The further technical scheme is as follows: the card support mechanism comprises a tray, a plurality of clamping grooves used for placing test cards are formed in the tray, elastic pieces are arranged in the clamping grooves, a movable blocking rod is arranged at one end of the tray, and a positioning pin is arranged at the other end of the tray.
The further technical scheme is as follows: the incubation detection mechanism comprises a support frame connected with the underframe, a temperature control heater, a Peltier, a cooling fan, an air guide pipe, an incubation heat conduction plate and a card support inspection microswitch; the incubation heat conducting plate is arranged on the upper surface of the supporting frame, the radiating fan is connected with the temperature control heater and the air guide pipe, the temperature control heater is positioned below the incubation heat conducting plate, radiating fins are further arranged on the temperature control heater, the Peltier paste is arranged between the incubation heat conducting plate and the radiating fins, the card support inspection microswitch is arranged on the incubation heat conducting plate, and the air guide pipe is adjacent to the temperature control heater.
The further technical scheme is as follows: the incubation heat-conducting plate is provided with a plurality of incubation areas, and each incubation area is internally provided with one card support inspection microswitch.
The further technical scheme is as follows: the upper surface of the Peltier is connected with the bottom surface of the incubation heat conduction plate, and the lower surface of the Peltier is connected with the upper surface of the radiating fin; the upper surface of the Peltier, the bottom surface of the incubation heat conduction plate and the joint of the lower surface of the Peltier and the upper surface of the radiating fin are provided with heat conduction silicone layers.
The further technical scheme is as follows: the sample feeding mechanism comprises a sample feeding box body, a sample feeding plate, a first driving mechanism, a second driving mechanism, a third driving mechanism and a fourth driving mechanism; the first driving mechanism and the fourth driving mechanism are arranged on one side of the sample injection box body, and the second driving mechanism and the third driving mechanism are arranged on the other side of the sample injection box body; the sample feeding plate is connected with the sample feeding box body and is positioned at the upper end of the sample feeding box body.
The further technical scheme is as follows: the sample feeding plate is provided with a sample rack sample feeding area, a bar code scanning completion area and a sample feeding area; the first driving mechanism is used for pushing the sample rack from the sample loading area of the sample rack to the barcode scanning area, the second driving mechanism is used for pushing the sample rack from the barcode scanning area to the barcode scanning completion area, the third driving mechanism is used for pushing the sample rack from the barcode scanning completion area to the sample loading area, and the fourth driving mechanism is used for pushing the sample rack from the sample loading area back to the sample loading area of the sample rack; the sampling box body is close to a scanning module support is arranged at the position of the bar code scanning area, and a scanning module is arranged on the scanning module support.
The further technical scheme is as follows: and a first reflective sensor is arranged at a position, close to the barcode scanning area, on the sample feeding plate, and a second reflective sensor is also arranged at a position, close to the sample adding area, on the sample feeding plate.
The further technical scheme is as follows: the first driving mechanism and the third driving mechanism have the same structure; the first driving mechanism comprises a motor supporting plate, a first motor, a first belt, a first push rod part, a first guide rail, a first idler wheel part and a first optical coupler part; the first motor is fixed on the side surface of the sample injection box body, the first guide rail, the first idler wheel part and the first optical coupler part are all connected with the sample injection box body, and the first motor is in transmission connection with the first push rod part through the first belt and forms reciprocating motion with the first guide rail, the first idler wheel part and the first optical coupler part; the second driving mechanism and the fourth driving mechanism have the same structure; the second driving mechanism comprises a second motor, a second belt, a second push rod part, a second guide rail, a second idler wheel part and a second optical coupler part; the second motor is fixed on the side surface of the sample injection box body and is adjacent to the first motor; the second guide rail, second idler portion, and second opto-coupler portion all with advance kind box hookup, the second motor passes through the second belt with second push rod portion transmission hookup, and with second guide rail, second idler portion, and second opto-coupler portion constitute reciprocating motion.
The further technical scheme is as follows: the humidity control mechanism comprises an accelerated convection fan, a drying module, an ultrasonic atomizer and a water bottle; the drying module is connected with the accelerated convection fan, the ultrasonic atomizer is connected with the water bottle, and the water bottle is adjacent to the drying module; the drying module is also provided with a plurality of grids.
Compared with the prior art, the utility model beneficial effect be: the test items can be randomly combined, so that the condition of large disposable sample quantity can be met, the condition of small and frequent samples can be adapted, the adaptability is strong, and the application scene is flexible; the device has the advantages of high measurement accuracy and efficiency, reduced design cost and production cost, realization of constant humidity in the incubation and test processes of the test card by adopting the humidity control mechanism, and improvement of the stability and accuracy of measurement.
The invention is further described with reference to the accompanying drawings and specific embodiments.
Drawings
FIG. 1 is a diagram of a fluorescence immunoassay analyzer according to the present invention;
FIG. 2 is a block diagram of a card holder structure;
FIG. 3 is a view showing the structure of an incubation detection mechanism;
FIG. 4 is a schematic diagram of the exploded structure of FIG. 3;
FIG. 5 is a structural view of a sample injection mechanism;
FIG. 6 is a first schematic diagram of the exploded structure of FIG. 5;
FIG. 7 is a second schematic diagram of the exploded structure of FIG. 5;
FIG. 8 is a block diagram of a moisture control mechanism;
fig. 9 is a schematic diagram of the exploded structure of fig. 8.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention will be described in further detail with reference to the accompanying drawings and the following detailed description.
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those skilled in the art without creative efforts belong to the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.
In the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "secured" are to be construed broadly and can, for example, be connected or detachably connected or integrated; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
In the present disclosure, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may comprise direct contact between the first and second features, or may comprise contact between the first and second features not directly. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above should not be understood to necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples described in this specification can be combined and combined by one skilled in the art.
As shown in fig. 1 to fig. 9, the present invention discloses a fluorescence immunoassay analyzer, which comprises a bottom frame 10, a sampling quantitative mechanism 20 disposed on the bottom frame 10, a card support mechanism 30, an incubation detection mechanism 40, a humidity control mechanism 50, and a sample introduction mechanism 60; the sampling quantitative mechanism 20 is arranged at the rear side of the incubation detection mechanism 40, the sample injection mechanism 60 is arranged at one side of the incubation detection mechanism 40, the humidity control mechanism 50 is arranged between the incubation detection mechanism 40 and the sample injection mechanism 60, and the card support mechanism 30 is arranged at the upper end of the incubation detection mechanism 40; the sampling and quantifying mechanism 20 includes a mounting frame 21 coupled to the base frame 10, a mechanical arm 22 capable of three-axis transmission is disposed on the mounting frame 21, and a quantifying pump (not shown), a barcode scanner (not shown), and an optical measuring device (not shown) are disposed on the mechanical arm 22.
As shown in fig. 1, the fluorescence immunoassay analyzer is divided into 5 modules: the device comprises a sampling quantifying mechanism 20, a card support mechanism 30, an incubation detection mechanism 40, a humidity control mechanism 50 and a sample injection mechanism 60, wherein all areas are clearly distributed and obviously modularized, a diluent placing area, a reaction cup placing area and a Tip placing area are further arranged beside the humidity control mechanism 50, various consumable materials are arranged in a partitioned mode, the bottom of the incubation detection mechanism 40 is easily expanded to be a temperature control heating area while the test card is placed on the temperature control heating area, the bottom of the test card is tightly attached to a temperature control plane, temperature control is carried out through solid-state heat conduction, efficiency is high, and temperature control is accurate; in the embodiment, the test cards are arranged in two rows, each row comprises 15 test cards, and 30 test cards can be simultaneously loaded, wherein the number of the test cards can be increased or reduced according to design requirements; the test cards are loaded through the card slots in the card support mechanism 30, each card slot can be loaded with different numbers of test cards, in this embodiment, each card slot is loaded with 5 test cards, and after the test cards are loaded into the card slots, the card slots are placed on the temperature control plane of the incubation detection mechanism 40 for incubation; the sampling quantitative mechanism 20 is an XYZ three-axis transmission mechanism, can move in 3 dimensions, and is a precise quantitative pump mounted on a Z axis (mechanical arm), the quantitative pump moves to the sample injection mechanism 60 along with the mechanism to suck the sample, can also move to a diluent placement area to suck the diluent and mix the sample and the diluent, and can also suck the mixed sample and then move to the incubation detection mechanism 40 to add the sample to the sample adding part of the test card to react; the Z-axis of the sampling and quantifying mechanism 20 is further equipped with a barcode scanner and an optical measuring device, the barcode scanner can scan and read barcodes on all test cards along with the movement of the XY two dimensions, and the optical measuring device can scan reaction areas on the test cards to obtain fluorescence measurement signals.
Specifically, as shown in fig. 2, the card support mechanism 30 includes a tray 31, a plurality of card slots 32 for placing test cards are provided on the tray 31, spring pieces 33 are provided in the card slots 32, one end of the tray 31 is provided with a movable stop lever 34, and the other end is provided with a positioning pin 35; the plurality of card slots 32 can be loaded with a plurality of types of test cards, that is, each type of test card can be placed in one card slot, and the test cards can be used randomly according to requirements out of sequence when used; the elastic sheet 33 can abut against and press the test card placed in the card slot 32 to limit the displacement of the test card in the left-right direction, in the embodiment, the stop lever 34 is connected with the tray 31 by a magnet, the locking function is realized when the stop lever 34 is closed, the test card can be inserted into and pulled out of the card slot 32 randomly when the stop lever 34 is opened, and the test card which is not inserted into the card slot can be pushed to the right when the stop lever 34 is closed; the positioning pins 36 serve as a guide and limit to accurately guide and limit the tray 31 to the incubation detection mechanism 40.
Specifically, as shown in fig. 3 to 4, the incubation detection mechanism 40 includes a support 41 coupled to the chassis 10, a temperature-controlled heater 42, a peltier 43, a heat-dissipating fan 44, an air duct 45, an incubation heat-conducting plate 46, and a card support inspection microswitch 47; the incubation heat conducting plate 46 is arranged on the upper surface of the supporting frame 41, the cooling fan 44 is connected with the temperature control heater 42 and the air guide pipe 45, the temperature control heater 42 is positioned below the incubation heat conducting plate 46, the temperature control heater 42 is further provided with a cooling fin 48, the peltier 43 is arranged between the incubation heat conducting plate 46 and the cooling fin 48, the card support inspection microswitch 47 is arranged on the incubation heat conducting plate 46, and the air guide pipe 45 is adjacent to the temperature control heater 42.
The incubation heat conduction plate 46 is provided with a plurality of incubation areas, each incubation area is provided with one card support inspection microswitch 47, in the embodiment, there are 6 incubation areas, each area is provided with one card support inspection microswitch 47, and the card support is placed on the incubation area and can press the card support inspection microswitch 47 to trigger a signal of the card support computer.
In the present embodiment, the heat dissipation fan 44 is disposed on the side surface of the temperature-controlled heater 42 and the back surface of the air guiding pipe 45 for guiding away heat.
In this embodiment, the peltier 43 is used as a temperature control element, the peltier 43 can realize heating or cooling of a temperature control surface by switching the direction of current, the upper surface of the peltier 43 is connected to the bottom surface of the incubation heat conduction plate 46, and the lower surface is connected to the upper surface of the heat dissipation fin 48; the upper surface of the peltier device 43 and the bottom surface of the incubation heat conduction plate 46, and the joint of the lower surface of the peltier device 43 and the upper surface of the temperature control heater 42 are provided with heat conduction silicone layers (not shown in the figure), so that the heat conduction speed is improved; in the present embodiment, the peltier device 43 is connected to the incubation heat conduction plate 46 and the heat dissipation plate 48 by adhesion; the side of the peltier element 20 that is attached to the incubation thermal plate 46 may be either hot or cold.
Specifically, as shown in fig. 5 to 7, the sample injection mechanism 60 includes a sample injection box 61, a sample injection plate 62, a first driving mechanism 63, a second driving mechanism 64, a third driving mechanism 65, and a fourth driving mechanism 66; the first driving mechanism 63 and the fourth driving mechanism 66 are arranged on one side of the sample box body 61, and the second driving mechanism 64 and the third driving mechanism 65 are arranged on the other side of the sample box body 61; the sample inlet plate 62 is connected with the sample inlet box 61 and is located at the upper end of the sample inlet box 61.
Wherein, a sample rack sample loading area 621, a barcode scanning area 622, a barcode scanning completion area 623 and a sample loading area 624 are arranged on the sample inlet plate 62; the first driving mechanism 63 is used for pushing the sample rack from the sample rack loading area 621 to the barcode scanning area 622, the second driving mechanism 64 is used for pushing the sample rack from the barcode scanning area 622 to the barcode scanning completion area 623, the third driving mechanism 65 is used for pushing the sample rack from the barcode scanning completion area 623 to the sample loading area 624, and the fourth driving mechanism 66 is used for pushing the sample rack from the sample loading area 624 back to the sample rack loading area 621; the sample introduction and the sample pushing are in the same area, the space for placing the sample rack is greatly reduced, the occupied space is small, the sample introduction device is short, the area is small, the movement time of the sample rack is short, the consumed time is short, and therefore the detection efficiency is improved. A scanning module bracket 67 is arranged at the position of the sample introduction box body 61 close to the barcode scanning area 622, and a scanning module 671 is arranged on the scanning module bracket; in this embodiment, the scanning module 671 is fixed on the scanning module bracket 67 through screws, so as to facilitate the bar code scanning of the sample on the sample rack, which is simple and convenient and has high efficiency.
A first reflective sensor 68 is disposed on the sample feeding plate 62 at a position close to the barcode scanning area 622, and a second reflective sensor 69 is further disposed on the sample feeding plate 62 at a position close to the sample loading area 624, in this embodiment, the first reflective sensor 68 and the second reflective sensor 69 are reflective optical coupling sensors; first reflective sensor 68 is used for detecting whether the sample frame reaches the position of bar code scanning, and second reflective sensor 69 is used for detecting whether the sample frame reaches the application of sample position, need not external force and only needs photoinduction, and is stable and effectual, and the fault rate is low.
The first driving mechanism 63 and the third driving mechanism 65 are identical in structure; the first driving mechanism 63 includes a motor support plate 631, a first motor 632, a first belt 633, a first push rod portion 634 (in this embodiment, the sample rack is in contact with the first push rod portion 634 or the second push rod portion 643 to perform pushing), a first guide rail 635, a first idler wheel portion 636, and a first optical coupler portion 637; motor support plate 631 passes through the screw fixation in advance the side of appearance box 61, first motor 632 passes through the screw fixation in motor support plate 631, first guide rail 635, first idler wheel portion 636, and first opto-coupler portion 637 all through the screw with advance the hookup of appearance box 61, first motor 632 passes through first belt 633 with first push rod portion 634 transmission hookup, and with first guide rail 635, first idler wheel portion 636, and first opto-coupler portion 637 constitute reciprocating motion to realize that first actuating mechanism 63 pushes away the position that the bar code scanned with the sample frame, the sample frame that third actuating mechanism 65 will scan finishes pushes away the position of application of sample.
Wherein, the second driving mechanism 64 and the fourth driving mechanism 66 have the same structure; the second driving mechanism 64 includes a second motor 641, a second belt 642, a second push rod 643, a second guide rail 644, a second idler gear 645, and a second optical coupler 646; the second motor 641 is fixed on the side surface of the sample injection box body 61 through a screw and is adjacent to the first motor 632, so that the space is saved; the second guide rail 644, the second idler wheel unit 645, and the second optical coupler unit 646 are all coupled to the sample feeding case 61 through screws, the second motor 641 is drivingly coupled to the second push rod unit 643 through the second belt 642, and forms a reciprocating motion with the second guide rail 644, the second idler wheel unit 645, and the second optical coupler unit 646, so as to realize that the second driving mechanism 64 continuously advances the sample rack, and the sample rack reaches the position of the scanning module 671 for sample barcode scanning, and the fourth driving mechanism 66 pushes the sample rack back to the initial position after the sample feeding is completed.
The sample injection box 61 is located outside the first motor 632 and the second motor 641, and a motor shield 70 is further disposed on the outside of the first motor 632 and the second motor 641, and the motor shield 70 is fixed on the side surface of the sample injection box 61 through screws for protecting the motors.
Wherein, still be equipped with a plurality of hexagonal screw-bolts 80 in the case 61 of advancing, in this embodiment, hexagonal screw-bolt 80 is hexagonal copper stud, and hexagonal copper stud passes through the screw fixation in case 61 of advancing for support into model 62, advance model 62 and pass through the screw fixation on hexagonal copper stud, simple and practical, the atress ability is strong.
Wherein, the upper portion of advancing kind box 61 still is equipped with side backup pad 90, it is located to advance the model 62 side the central point of side backup pad 90 puts, and side backup pad 90 passes through the screw fixation on advancing kind box 61, all be equipped with ascending bellying 91 around side backup pad 90 for direction and spacing sample frame, the apron of advancing kind box 61 simultaneously is equivalent to.
Specifically, as shown in fig. 8 to 9, the humidity control mechanism 50 includes an accelerated convection fan 51, a drying module 52, an ultrasonic atomizer 53, and a water bottle 54; the drying module 52 is coupled with the accelerated convection fan 51, the ultrasonic atomizer 53 is coupled with the water bottle 54, and the water bottle 54 is adjacent to the drying module 52; the drying module 52 is further provided with a plurality of grids 521.
The drying module 52 is filled with a drying agent, which may be a chemical drying agent, such as calcium sulfate, calcium chloride, etc., and is dried by combining with water to form a hydrate; physical desiccants are also possible. The surface of the drying module 52 is provided with a grid 521 window, so that the surface of the drying agent can be contacted with air, the drying agent has a color development function, and when the interior of the drying agent absorbs moisture, the drying agent can change color to remind a user of replacement; the drying module 52 is further provided with a heating module (not shown in the figure), which can heat the drying agent to analyze water in the drying agent, so as to realize cyclic utilization of the drying agent, the air outlet surface of the convection fan 51 is accelerated to be opposite to the grid 521 window on the surface of the drying module 52, convection and exchange between air and the surface of the drying agent are increased by accelerating the convection fan 51, so that the drying efficiency is improved, meanwhile, the convection fan 51 is also used for controlling humidity, when the humidity in the cavity is close to or reaches the target humidity, the convection fan 51 is accelerated to stop or reduce the air speed, so that the drying efficiency of the drying module 52 is reduced, exchange and drying of water vapor are realized only through natural convection of air, and the stability of the humidity in the cavity is maintained within a certain range; when the humidity in the cavity is too high or the humidity rises to exceed the control precision range requirement of the target humidity, the accelerated convection fan 51 is started to increase the air convection and drying effects; the control of humidity is realized through the synergistic effect of the drying module 52 and the accelerated convection fan 51; the drying module 52 further comprises an automatic heating and water removing function, after the drying agent in a single drying module 52 is saturated due to water absorption, the module can be taken out of the equipment cavity, the heater of the drying module 52 can be used for heating through inserting electricity, moisture absorbed in the drying agent can be separated out due to the heating effect, after the drying agent is heated for a certain time, the drying agent is recovered to a drying state, the drying agent can be placed into the equipment cavity again for repeated use, and the continuous work of humidity control of the equipment can be kept; this drying module 52 still includes the colour inductor (not shown in the figure), the colour inductor can continuously monitor the colour on drier surface (drying module 52 leaves transparent window, an acquisition for color sensor's signal), the colour that detects the interior drier of window when color sensor changes, can send the warning, remind the user to change this drying module 52, keep the drying effect of system, the advantage of this scheme lies in, carry out the drying through the synergism of accelerating convection fan 51 and drying module 52, form the inner loop drying of gaseous in the cavity, its drying efficiency is high, the power consumption is low, can not lead to the air heating effect, and is with low costs simultaneously.
The utility model discloses an ultrasonic atomization ware 53 humidification, ultrasonic atomization ware 53 utilizes the electron high frequency oscillation (oscillation frequency is 1.7MHz or 2.4MHz, exceeds the hearing scope of people, and this electron oscillation is absolutely harmless to human body and animal), through the high frequency resonance of ceramic atomization piece, breaks up the molecular bond between the liquid hydrone and produces the elegant water smoke naturally, need not heat or add any chemical reagent; the utility model humidifies the air in the equipment cavity by controlling the working on-off switch of the ultrasonic atomizer 53; when a humidity sensor of the equipment detects that the air humidity in the cavity is reduced and exceeds the precision range of the humidity target value, starting an atomizer to perform humidification operation, and improving the air humidity in the cavity; the drying module 52 and the ultrasonic atomizer 53 work alternately according to the signal of the humidity sensor, and when the humidity in the equipment cavity is too high, the drying module 52 starts to work to reduce the humidity in the cavity; when the humidity in the equipment cavity is too low, the ultrasonic atomizer 53 starts to work to increase the humidity in the cavity, the drying module 52 and the ultrasonic atomizer 53 work alternately to control the humidity in the equipment cavity within a proper humidity range, and the measurement stability and accuracy of the test card are improved.
The utility model has the characteristics of it is following:
1. adopt matrix test card loading structure, the measurement item can make up at random, compares the design of card lifting storehouse formula, the utility model discloses can carry out the test card of multiple test item and operate the computer simultaneously and measure according to the measurement demand, for example: 30 reagent cards which are arranged at one time can be used for operating 30 detection items simultaneously theoretically, the card bin type equipment is limited by the number of card bins, test cards with the same number as the number of the card bins can be operated at the same time at each time, the number of the card bins is limited by the structure and the volume, the number of the test cards is only 5 at most generally, and the number of the test cards is far lower than the design of the utility model;
2. in the matrix type incubation detection mechanism, the channels of each test card are identical, so that test items can be randomly combined and placed, and the item types and the number of the test cards can be flexibly matched;
3. the number of the test cards which are loaded on the machine at each time can be carried out according to actual test requirements, and the number of samples which are loaded on the machine is required to be tested, so that the problem of overdue waste caused by residual test cards of the card bin type equipment is solved, and storage designs such as extra constant humidity for a card bin and the like are not required to be added for storing the residual test cards;
4. the test card can be added at any time in the test process, so that the method can well adapt to the conditions of small quantity of samples and high frequency in emergency treatment and the like, therefore, the matrix type design scheme can meet the condition of large disposable sample quantity and can also adapt to the condition of small and frequent samples, the adaptability is strong, and the application scene is flexible;
5. the test cards are fixed and measured on the machine through the card slots, each card slot can be used for placing 5 test cards (the number can be modified according to actual needs or habits), after the test cards are inserted into the card slots, the whole card support mechanism is placed towards the incubation detection mechanism, the incubation detection mechanism is divided into a plurality of corresponding cells according to the number of the card slots, in the embodiment, the cells are divided into 6 cells (the number of the cells can be increased or reduced according to design requirements), each cell corresponds to the placement position of one card support, in order to enable the card supports to be placed easily and accurately, the structure corresponding to the card support at each cell position is designed with a guide slot and a magnetic attraction, when the card supports enter the guide structure, due to the effect of the magnetic attraction, the card supports can be automatically matched with the positioning structure, so that the card supports can be accurately and conveniently positioned in the cells, and the positioning precision is very high, the consistency of the channels among the test cards can be ensured, and the accuracy of measurement is ensured;
6. because the test card incubation detection mechanism adopts a matrix structure, and the equipment layout adopts a modular design, the test card incubation detection mechanism can be adapted to different types of reagent cards, such as a microfluidic chip, an integrated reagent card package, a gold-labeled test card and the like, and the equipment expansion and design compatibility is very convenient;
7. the sample introduction mechanism adopts a circulating sample introduction design, common equipment on the market generally adopts a transmission mode that a sample frame is turned left from the right or turned right from the left, the sample frame is fed from one end and then discharged from the other end, or adopts a structure that a plurality of sample frames are simultaneously fed in and discharged, the utility model adopts a sample frame circulating motion mode, after the sample frame to be detected is placed from the front, the sample frame is automatically fed into the equipment, after a test tube bar code is scanned, the sample frame is moved to a sampling position for sample mixing and sample suction, after the sample is sucked, the test tube frame is moved to a sample introduction position from the sample suction position to complete a cycle, the circulating sample introduction mechanism can reach the maximum sample introduction quantity of the sample frame, the common sample introduction mode that one end is fed in and one end is discharged or simultaneously fed in and discharged is in the same space, the sample frame quantity on one machine is less than the circulating sample introduction structure, the design of the sample feeding mechanism can effectively reduce the movement stroke of the sample frame and reduce the space of the sample feeding module, and meanwhile, the reliability of the sample feeding mechanism can be effectively improved due to the simple movement of the sample frame;
8. the utility model discloses a Tip head has inhaled the mode of appearance, adopts the mode of inhaling the sample to beat the whole blood sample simultaneously and carries out the mixing, because the sample size of whole blood sample is great, in order to can compromise mixing effect and inhale appearance precision, can operate the Tip head of multiple specification simultaneously, the utility model designs two kinds of Tip heads, a volume is 200ul (can also use the Tip head of other volumes according to the application needs) for absorb the mixed sample of Tip blood sample or having added the diluent, cooperation ration module, 200ul Tip head can accurately absorb the sample size of 5-150ul scope; in order to mix the whole blood sample, 1000ul of Tip is added, the Tip has a large volume, and can absorb a sufficient whole blood sample at one time, then the whole blood sample is pumped back into the sample tube, and the sample can be effectively mixed after repeated pumping, so that the design has the characteristics of no cross contamination and no liquid path for Tip head sampling, the problem that the uncapped sample tube cannot be shaken uniformly mechanically can be solved, the uncapped structure is not required to be designed, the design risk is reduced, and the design difficulty is reduced;
9. due to the adoption of a modularized design scheme, and the fact that all modules have the design characteristic of reusability, for example, the incubation detection mechanism is of a matrix structure, the number of cells can be increased or reduced according to the design requirement; the sample feeding mechanism adopts a circulating sample feeding mode, the number of the sample racks on the machine at one time is only related to the movement stroke of the sample racks, and the number of the sample racks on the machine at one time can be increased or reduced by changing the stroke without changing the scheme of the sample feeding mechanism; the number of the consumables on the disposable computer is changed by only increasing or reducing the number of the consumable boxes; under the condition of not changing the layout of the whole machine, the equipment can be modified by only increasing and reducing the unit number of the modules and changing the stroke of an XYZ axis of the sampling quantitative mechanism according to the layout condition, so that the equipment can be conveniently designed and upgraded and reused.
The technical content of the present invention is further described by the embodiments only, so that the reader can understand it more easily, but the embodiments of the present invention are not limited thereto, and any technical extension or re-creation according to the present invention is protected by the present invention. The protection scope of the present invention is subject to the claims.

Claims (10)

1. A fluorescence immunoassay analyzer is characterized by comprising an underframe, a sampling quantitative mechanism, a clamping and supporting mechanism, an incubation detection mechanism, a humidity control mechanism and a sample introduction mechanism, wherein the sampling quantitative mechanism, the clamping and supporting mechanism, the incubation detection mechanism, the humidity control mechanism and the sample introduction mechanism are arranged on the underframe; the sampling quantitative mechanism is arranged at the rear side of the incubation detection mechanism, the sample injection mechanism is arranged at one side of the incubation detection mechanism, the humidity control mechanism is arranged between the incubation detection mechanism and the sample injection mechanism, and the clamping and supporting mechanism is arranged at the upper end of the incubation detection mechanism; the sampling quantitative mechanism comprises a mounting frame connected with the underframe, a mechanical arm capable of realizing three-axis transmission is arranged on the mounting frame, and a quantitative pump, a bar code scanner and an optical measurer are arranged on the mechanical arm.
2. The fluorescence immunoassay analyzer of claim 1, wherein the card support mechanism comprises a tray, a plurality of slots for holding the test cards are arranged on the tray, spring pieces are arranged in the slots, a movable stop lever is arranged at one end of the tray, and a positioning pin is arranged at the other end of the tray.
3. The fluoroimmunoassay analyzer of claim 1, wherein the incubation detection mechanism comprises a support frame coupled to the chassis, a temperature-controlled heater, a peltier, a heat-dissipation fan, an air duct, an incubation heat-conducting plate, and a card-holder inspection microswitch; the incubation heat conducting plate is arranged on the upper surface of the supporting frame, the radiating fan is connected with the temperature control heater and the air guide pipe, the temperature control heater is positioned below the incubation heat conducting plate, radiating fins are further arranged on the temperature control heater, the Peltier paste is arranged between the incubation heat conducting plate and the radiating fins, the card support inspection microswitch is arranged on the incubation heat conducting plate, and the air guide pipe is adjacent to the temperature control heater.
4. The fluoroimmunoassay analyzer of claim 3, wherein the incubation heat-conducting plate is provided with a plurality of incubation areas, and each of the incubation areas is provided with one of the card holder inspection micro-switches.
5. The fluoroimmunoassay analyzer of claim 4, wherein an upper surface of the Peltier is coupled to a bottom surface of the incubation heat-conducting plate, and a lower surface is coupled to an upper surface of the heat sink; the upper surface of the Peltier, the bottom surface of the incubation heat conduction plate and the joint of the lower surface of the Peltier and the upper surface of the radiating fin are provided with heat conduction silicone layers.
6. The fluoroimmunoassay analyzer of claim 1, wherein the sample introduction mechanism comprises a sample introduction chamber, a sample introduction plate, a first driving mechanism, a second driving mechanism, a third driving mechanism, and a fourth driving mechanism; the first driving mechanism and the fourth driving mechanism are arranged on one side of the sample injection box body, and the second driving mechanism and the third driving mechanism are arranged on the other side of the sample injection box body; the sample feeding plate is connected with the sample feeding box body and is positioned at the upper end of the sample feeding box body.
7. The fluoroimmunoassay analyzer of claim 6, wherein the sample inlet plate is provided with a sample rack loading area, a barcode scanning completion area, and a sample loading area; the first driving mechanism is used for pushing the sample rack from the sample loading area of the sample rack to the barcode scanning area, the second driving mechanism is used for pushing the sample rack from the barcode scanning area to the barcode scanning completion area, the third driving mechanism is used for pushing the sample rack from the barcode scanning completion area to the sample loading area, and the fourth driving mechanism is used for pushing the sample rack from the sample loading area back to the sample loading area of the sample rack; the sampling box body is close to a scanning module support is arranged at the position of the bar code scanning area, and a scanning module is arranged on the scanning module support.
8. The fluoroimmunoassay analyzer of claim 7, wherein a first reflective sensor is disposed on the sample inlet plate near the barcode scanning area, and a second reflective sensor is disposed on the sample inlet plate near the sample application area.
9. The fluoroimmunoassay analyzer of claim 8, wherein the first and third drive mechanisms are identical in construction; the first driving mechanism comprises a motor supporting plate, a first motor, a first belt, a first push rod part, a first guide rail, a first idler wheel part and a first optical coupler part; the first motor is fixed on the side surface of the sample injection box body, the first guide rail, the first idler wheel part and the first optical coupler part are all connected with the sample injection box body, and the first motor is in transmission connection with the first push rod part through the first belt and forms reciprocating motion with the first guide rail, the first idler wheel part and the first optical coupler part; the second driving mechanism and the fourth driving mechanism have the same structure; the second driving mechanism comprises a second motor, a second belt, a second push rod part, a second guide rail, a second idler wheel part and a second optical coupler part; the second motor is fixed on the side surface of the sample injection box body and is adjacent to the first motor; the second guide rail, second idler portion, and second opto-coupler portion all with advance kind box hookup, the second motor passes through the second belt with second push rod portion transmission hookup, and with second guide rail, second idler portion, and second opto-coupler portion constitute reciprocating motion.
10. The fluoroimmunoassay analyzer of claim 1, wherein the humidity control mechanism comprises an accelerated convection fan, a drying module, an ultrasonic atomizer, and a water bottle; the drying module is connected with the accelerated convection fan, the ultrasonic atomizer is connected with the water bottle, and the water bottle is adjacent to the drying module; the drying module is also provided with a plurality of grids.
CN201921729645.3U 2019-10-15 2019-10-15 Fluorescence immunoassay appearance Active CN211348255U (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115825463A (en) * 2023-02-17 2023-03-21 世纪亿康(天津)医疗科技发展有限公司 Blood coagulation detection equipment

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115825463A (en) * 2023-02-17 2023-03-21 世纪亿康(天津)医疗科技发展有限公司 Blood coagulation detection equipment

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