CN211339537U - Algae continuous culture reaction system - Google Patents

Algae continuous culture reaction system Download PDF

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Publication number
CN211339537U
CN211339537U CN201921919889.8U CN201921919889U CN211339537U CN 211339537 U CN211339537 U CN 211339537U CN 201921919889 U CN201921919889 U CN 201921919889U CN 211339537 U CN211339537 U CN 211339537U
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algae
photobioreactor
tank
led chip
closed reactor
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CN201921919889.8U
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叶华
关辉
邹立红
易启甲
史会帅
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Anhui Debao Biotechnology Co ltd
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Anhui Debao Biotechnology Co ltd
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Abstract

The utility model relates to an algae continuous culture reaction system, continuous culture reaction system includes two at least photobioreactors, culture solution case and algae kind case, two the photobioreactors are first photobioreactor and second photobioreactor respectively, algae kind case is through first pipe connection first photobioreactor, provides the algae liquid that contains the algae kind for first photobioreactor, and the culture solution case provides nutrient substance for the different growth stages of algae through second pipeline parallel connection first photobioreactor and second photobioreactor, first photobioreactor passes through third pipe connection second photobioreactor. The light bioreactor is internally provided with a waterproof LED lamp, and the waterproof LED lamp comprises an LED chip, a transparent lampshade, a heat dissipation part, an exhaust part and a waterproof device.

Description

Algae continuous culture reaction system
Technical Field
The utility model belongs to the technical field of algae culture equipment, concretely relates to algae continuous culture reaction system.
Background
Microalgae is an autotrophic plant with rich nutrition and high photosynthetic utilization rate, biomass of the microalgae contains various biochemical substances, such as polysaccharide, protein, grease, carbohydrate, pigment and the like, the microalgae is widely distributed on land and sea, and the microalgae has wide development prospects in the fields of food, medicine, genetic engineering, liquid fuel and the like. The microalgae has high growth speed and strong environmental adaptability, and can be subjected to photosynthetic self-culture in a photobioreactor. Haematococcus pluvialis is a unicellular green alga in which a large amount of astaxanthin is accumulated in cells of Haematococcus pluvialis under conditions of nitrogen deficiency, phosphorus deficiency or irradiation with intense light energy. One of the strongest natural antioxidants of natural astaxanthin in the world can effectively remove oxygen free radicals in cells, enhance the regeneration capacity of the cells, maintain the balance of organisms, reduce the accumulation of aging cells and protect the health of cells and DNA from inside to outside, thereby protecting the health of skin, promoting the growth of hair, resisting aging, relieving sports fatigue and enhancing the vitality.
At present, the large-scale culture of haematococcus pluvialis mainly adopts a runway type culture pond utilizing outdoor natural light, and the main factors hindering the runway type large-scale production of the haematococcus pluvialis are biological pollution and the problem of overhigh cost caused by low yield per unit area due to low population density in the process of culturing the haematococcus pluvialis. Haematococcus pluvialis cultured by using a biological reaction kettle consisting of a pipeline type photo-biological reaction kettle or a small system has the defects that the pipeline wall is easily adhered by algae cells to cause light transmittance reduction, temperature instability and difficulty in cleaning during the culture, so that the method is only suitable for small-scale production in a laboratory.
The growth process of various algae including haematococcus pluvialis is divided into different stages, the growth environments required by the different stages are different, the cultivation requirements of the algae in the different growth stages are met, the cultivation efficiency and the automation degree are improved, and an industrial large-scale cultivation system is established, which is always a problem researched by technical personnel in the field.
SUMMERY OF THE UTILITY MODEL
In order to the above problem, the utility model provides an algae continuous culture reaction system, continuous culture reaction system includes two at least photobioreactors, culture solution case and algae kind case, two the photobioreactors are first photobioreactor and second photobioreactor respectively, algae kind case is through first pipe connection first photobioreactor, provides the algae liquid that contains the algae kind for first photobioreactor, and the culture solution case provides nutrient material for the different growth stages of algae through second pipeline parallel connection first photobioreactor and second photobioreactor, first photobioreactor passes through third pipe connection second photobioreactor.
The photobioreactor includes the airtight reactor jar body, the internal portion of airtight reactor jar is equipped with waterproof LED lamp, and the external portion of airtight reactor jar is equipped with LED drive controller, waterproof LED lamp includes LED chip, transparent lamp shade, radiating part, exhaust part and watertight fittings, the surface at the radiating seat of radiating part is established to the LED chip, and LED chip and radiating part establish inside transparent lamp shade, watertight fittings establishes the top at transparent lamp shade, the LED chip can send the light that is suitable for the multiple wavelength range of algae growth, the energy of LED drive controller control LED chip increases simultaneously or reduces.
The closed reactor tank body is selected from a vertical type or a horizontal type, and preferably, the closed reactor tank body is a vertical container. More preferably, the closed reactor tank is a cylinder, more preferably, the diameter of the closed reactor tank is 10-150cm, the height of the closed reactor tank is 150-280cm, and a proper cylinder size can be selected according to the actual requirement of algae cultivation. The material of the closed reactor tank body is selected from stainless steel, plastic or organic glass, and preferably, the material of the closed reactor tank body is stainless steel.
Preferably, the top of the airtight reactor tank body is provided with a detachable face cover, the face cover can be provided with various joints, the face cover is connected and sealed with the airtight reactor tank body through a buckle, more preferably, a sealing ring and a flange are arranged between the face cover and the cylinder body, so that the face cover and the airtight reactor tank body can be conveniently and fixedly connected, and the sealing performance of the photobioreactor is improved.
Preferably, the outer wall of the closed reactor tank body is provided with a sight glass, so that the growth condition of algae in the closed reactor tank body can be observed conveniently.
Preferably, a support seat is arranged below the closed reactor tank body and used for supporting and stabilizing the closed reactor tank body, and a space is reserved between the bottom of the closed reactor tank body and the ground so as to facilitate discharge of the algae liquid or the products in the closed reactor tank body. In a specific embodiment of the present invention, the supporting seat is four supporting legs, and is disposed at the periphery of the bottom of the closed reactor tank body for fixedly supporting the closed reactor tank body.
The airtight reactor tank body comprises at least one waterproof LED lamp, a support and a condenser pipe, wherein the waterproof LED lamp and the condenser pipe are fixed on the support, the waterproof LED lamp comprises an LED chip, a transparent lampshade, a radiating part, an exhaust part and a waterproof device, and the waterproof device is installed at the top of the transparent lampshade.
The waterproof LED lamp is in a long strip shape and is vertically placed inside the sealed reactor tank body, the waterproof device of the waterproof LED lamp is located at the upper part or the top of the sealed reactor tank body, and preferably, the waterproof device is located above the liquid level of the algae liquid.
The arrangement position of the waterproof LED lamp in the closed reactor tank body is selected from the upper part, the middle part and the lower part of the closed reactor tank body, and preferably, the waterproof LED lamp is arranged in the middle of the closed reactor tank body.
The heat dissipation part comprises colorless transparent silicone oil and a heat dissipation seat, the colorless transparent silicone oil is filled in the transparent lampshade, and the heat dissipated by the LED chip is conducted out of the transparent lampshade.
The heat dissipation seat and the LED chip are immersed in the colorless transparent silicone oil, the silicone oil can absorb heat emitted when the LED chip emits light, the heat dissipation performance of the waterproof LED lamp is improved, the waterproof LED lamp can bear high power or work for a long time, in addition, the transmission fidelity rate of the silicone oil to light is better than that of air, and light emitted by the LED chip is not influenced.
The radiating seat is long-strip barrel-shaped, the cross section of the radiating seat is polygonal, the LED chips are installed on the radiating seat, and preferably, the LED chips are installed on the outer surface of the radiating seat facing to different directions in rows. Preferably, the lamp pieces are respectively arranged on the outer surfaces of the heat radiating seat facing different directions, and the LED chips are mounted on the lamp pieces.
Preferably, the LED chips are arranged in rows on the outer surface of the heat dissipation seat, the LED chips with different wavelengths are arranged in a staggered mode, and the arrangement density of the LED chips is reasonably arranged according to the actual length of the heat dissipation seat and the cultured algae quantity of the photobioreactor. For example, one row or two rows of the LED chips are disposed on the outer surface of the heat sink, wherein the first LED chip and the second LED chip are staggered, or the first LED chip and the third LED chip are staggered, or the second LED chip and the third LED chip are staggered, or the first LED chip, the second LED chip, and the third LED chip are staggered in any order.
Preferably, the LED chips with different wavelengths are arranged on the same surface of the heat radiating seat, so that illumination of 1-360 degrees is realized. LED chips on the same surface of the heat radiating seat are connected in series or in parallel, after the circuits of the chips are gathered, the chips enter the heat radiating seat from an opening at the bottom of the heat radiating seat, then the chips stretch out of the waterproof device from an opening at the top of the heat radiating seat, and then the chips are connected with an external power supply or a control system, and a waterproof insulating layer is sleeved outside the wires of the circuits.
The material of radiating seat is the metal that the heat conductivity is good, and is preferred, the material of radiating seat is metallic aluminum, is favorable to the LED chip heat dissipation.
The exhaust part is communicated with the inside and the outside of the transparent lampshade and used for guiding out the gas expanded when the inside of the transparent lampshade is heated out of the waterproof LED lamp, so that the safe use of the waterproof LED lamp is ensured. Preferably, the exhaust part is an exhaust pipe, the exhaust pipe is arranged in a waterproof circuit of the LED chip, and the waterproof circuit can penetrate out of the tank body of the closed reactor and is connected with an external power supply.
Colorless transparent silicone oil is heated the back volume expansion, and simultaneously, the inside air of transparent lamp shade is heated and also can be expanded, the blast pipe can with unnecessary air in the transparent lamp shade is derived, prevents transparent lamp shade internal pressure is too big.
The transparent lampshade protects the LED chip and the circuit and isolates the algae liquid from the LED chip. The transparent lampshade is in a strip barrel shape, the bottom of the transparent lampshade is sealed, the top of the transparent lampshade is provided with an opening, and the opening in the top allows the heat dissipation seat, the circuit and the colorless transparent silicone oil to enter and exit the transparent lampshade.
The waterproof device is arranged at the top opening of the transparent lampshade, seals the top opening and allows the circuit of the LED chip to penetrate out of the waterproof device. For example, the waterproof device is a silicone cap and is provided with a clamp which provides a locking seal.
And the circuit of the waterproof LED lamp extends out of the closed reactor tank body through the waterproof connector and then is connected with the LED drive controller.
The LED chip can emit light in a plurality of wavelength ranges selected from the following two or more combined wavelength ranges: 390-420nm, 440-470nm or 640-680 nm.
Preferably, the LED chip comprises three chips with different wavelengths, the LED chip comprises a first LED chip, a second LED chip and a third LED chip, the wavelength of light emitted by the first LED chip is 390-420nm, the wavelength of light emitted by the second LED chip is 440-470nm, and the wavelength of light emitted by the third LED chip is 640-680nm, so that different requirements of the algae on the wavelength of light energy at different growth stages are met.
Preferably, the irradiation radius of the waterproof LED lamp is 5-20cm, and more preferably, the irradiation radius of the waterproof LED lamp is 10-15 cm. The irradiation radius of the waterproof LED lamp is adjusted according to the density of algae liquid or the quantity of cultured algae, so that algae cells at all positions in the tank body of the closed reactor can receive enough light energy, and illumination dead corners caused by overlarge density of the algae liquid or too much algae are avoided. Preferably, the waterproof LED lamps are uniformly arranged in the tank body of the closed reactor.
The photobioreactor further includes an LED drive controller that controls power of the LED chips to be simultaneously increased or decreased. The LED driving controller controls the power of the LED chip so as to control the energy of the LED chip, and can be matched with different requirements of algae cultivation on the light energy.
The LED driving controller can adjust the light quantum flux density of a single waterproof LED lamp to be 1 × 104-8×105μmol/m2S, preferably, the photon flux density is 4 × 105-6×105μmol/m2S. The working time of the waterproof LED lamp is a culture period for the rapid growth and proliferation of algae cells, the culture period is 60-300 hours, preferably, the culture period is 60-150 hours, and more preferably, the culture period is 60-100 hours. The total power of the waterproof LED lamp is 60-15000W.
Preferably, the photobioreactor further comprises a power transmitter for measuring the power of the waterproof LED lamp.
Through LED drive controller regulates and control power or energy, the illumination time of waterproof LED lamp, can provide different light quantum flux density and illumination cycle for the different growth stage of haematococcus pluvialis, the utility model provides a mutually supporting of light quantum flux density, the condition of cultivation cycle provides the illumination condition that is fit for the haematococcus pluvialis growth, can improve the speed that haematococcus pluvialis grows by a wide margin, shortens growth cycle, improves the quality and the quantity of accumulation astaxanthin. On the other hand, waterproof LED lamp sets up the design of the above-mentioned specific illumination condition of density cooperation, provides all-round, the adjustable illumination environment of multidimension degree for haematococcus pluvialis, replaces traditional natural lighting or fluorescent lamp to shine completely, has overcome the adverse effect that natural lighting receives conditions such as weather, season, sunshine time, breed pond degree of depth.
The support comprises an upper support and a lower support, the upper support fixedly supports the middle upper part of the waterproof LED lamp, the lower support fixedly supports the middle lower part of the waterproof LED lamp, preferably, the upper support fixedly supports the upper part of the waterproof LED lamp, and the lower support fixedly supports the bottom of the waterproof LED lamp. The utility model discloses an in a specific embodiment, upper bracket and lower carriage all have vertically and horizontally staggered's crossbeam, are equipped with the rand at the intersection of two crossbeams, the rand is used for cup jointing and fastening waterproof LED lamp.
Preferably, the rack further includes an intermediate rack which is coupled to support the condensation duct, and the intermediate rack may take various forms, for example, a longitudinal support bar and/or a lateral support bar provided between the upper rack and the lower rack.
The closed reactor tank body comprises at least one condensing pipe, the shape of the condensing pipe is selected from one or the combination of more than two of linear shape, U-shaped, spiral shape or snake line shape, and preferably, the shape of the condensing pipe is spiral shape. The condenser pipe is arranged at one or more than two positions of the upper part, the middle part and the lower part of the closed reactor tank body and is immersed below the liquid level of the algae liquid, preferably, the condenser pipe is arranged at the upper part, the middle part and the lower part of the closed reactor tank body; preferably, the condensation pipe corresponds to the position of the waterproof LED lamp.
Preferably, the number of the condensation pipes is 2-10, and the condensation pipes can be arranged in the closed reactor tank body from top to bottom or arranged in the closed reactor tank body in an array or a square matrix manner. In one embodiment of the present invention, 5 spiral condensation pipes are disposed in the closed reactor tank and arranged in the order from top to bottom.
And cooling water is introduced into the condensation pipe, so that a large amount of heat emitted by the waterproof LED lamp and redundant heat released in the algae growth and metabolism process can be taken out of the closed reactor tank body, and the temperature inside the closed reactor tank body is always kept in a temperature range suitable for algae growth and metabolism. The condenser pipe adjusts the temperature in the closed reactor tank body to be 15-30 ℃, and preferably, the temperature is 20-25 ℃. The condenser pipe is used for adjusting the temperature and combining with the multi-dimensional adjustable illumination condition provided by the waterproof LED lamp, so that a suitable growth environment is provided for algae, and the growth of the algae and the accumulation of astaxanthin are promoted.
And the inlet and the outlet of the condensation pipe are respectively connected with a cold water inlet pipe and a cold water outlet pipe, the cold water inlet pipe and the cold water outlet pipe are connected to a cooling water connector on the outer wall of the closed reactor tank body, and the cooling water connector is connected to a water chiller through a pipeline and a first valve. Preferably, the cooling water joint is arranged at the upper part of the closed reactor tank body.
When a plurality of condensation pipes are arranged, the inlet and the outlet of each condensation pipe can be respectively connected with the cold water inlet pipe and the cold water outlet pipe in a serial or parallel mode, and then water is supplied or discharged from the cooling water joint in a unified mode.
The closed reactor is characterized in that at least one temperature probe is arranged in the closed reactor tank body, the temperature probe is immersed below the liquid level of algae liquid, a circuit of the temperature probe extends out of the closed reactor tank body through a waterproof connector and then is connected with a PLC (programmable logic controller), preferably, the PLC is connected with the water chiller, when the temperature in the closed reactor tank body is high, the control system starts the water chiller, the movement of cooling water in a condensation pipe takes away surplus heat, and when the temperature in the closed reactor tank body is low, the control system stops the water chiller.
The photobioreactor also comprises an air supply device which provides carbon dioxide and air required by the growth of algae for the closed reactor tank body. The gas supply device comprises a sterile filter, a gas flow regulator, a first gas joint and a second gas joint, wherein the carbon dioxide gas source and the air gas source are connected with the sterile filter or the gas flow regulator and then connected with the first gas joint, the sequence of the sterile filter and the gas flow regulator can be randomly exchanged, the first gas joint is used as a gas inlet, the second gas joint is used as a gas outlet, and the first gas joint and the second gas joint are arranged on the outer wall of the closed reactor tank body and communicated with the inside of the closed reactor tank body. The gas flow regulator comprises a carbon dioxide flow meter and an air flow meter, and the flow rates of the carbon dioxide and the air are respectively regulated.
When the device is used, carbon dioxide and air control the flow through the carbon dioxide flow meter and the air flow meter, then enter the tank body of the closed reactor through the first gas joint, are subjected to algae growth and metabolism utilization, then pass through the sterile filter from the second gas joint, are subjected to sterilization treatment, and finally are discharged out of the tank body of the closed reactor.
For example, the carbon dioxide gas source is industrial carbon dioxide, the air gas source is compressed air formed by air passing through an air compressor, and the carbon dioxide is used as a carbon source required by algae growth.
The positions of the first gas joint and the second gas joint are selected from a surface cover of a closed reactor tank body, the middle part of the tank body or the bottom of the tank body.
And the carbon dioxide and the air can be independently introduced into the tank body of the closed reactor after being filtered.
The device comprises a closed reactor tank body and is characterized in that at least one pH probe is arranged in the closed reactor tank body, the pH probe is immersed in algae liquid and is connected with a pH controller outside the closed reactor tank body through a line and a waterproof joint, and the pH controller is connected with a carbon dioxide flowmeter and an air flowmeter through a PLC (programmable logic controller) control device. And the pH controller and the PLC control system control the flow of the carbon dioxide supplied to the tank body of the closed reactor to increase or decrease according to the increase or decrease of the pH value detected by the pH probe. The pH probe is used for monitoring whether the pH value of the algae liquid is suitable for the growth of algae in real time, the algae can generate various nutrients and excrement in the growth and metabolism processes, the substances are partially released into the algae liquid and can change the pH value of the algae liquid, and the pH value of the algae liquid is a key factor influencing the growth and metabolism of the algae.
Preferably, the number of the pH probes is 2-5, so that the pH values of the algae liquid at different positions can be monitored conveniently.
The setting position of the pH probe is selected from the upper part, the middle part and the lower part of the inside of the tank body of the closed reactor, and preferably, the pH probe is arranged in the middle part of the inside of the tank body of the closed reactor.
The utility model combines the pH value of the algae liquid with the carbon source carbon dioxide which is necessary for the growth of the algae to regulate and control. Because carbon dioxide dissolves in behind the algae liquid, the algae liquid shows acidity, and pH value reduces, the utility model discloses establish the contact of carbon dioxide and pH value from this, through real-time supervision algae liquid pH value, the flow of linkage adjustment carbon dioxide, and then reach the dual purpose of control algae carbon source supply volume and algae liquid pH value simultaneously.
The utility model discloses discover that the pH value control of algae liquid is in 6-8 within ranges, is favorable to the algae to grow, and equal maximize promotion algae growth is supplied with to algae liquid pH value and carbon dioxide carbon source simultaneously, and is preferred, the pH value is 6.5-7. Meanwhile, the flow rate of the carbon dioxide is 0.05-1L/min, preferably, the flow rate of the carbon dioxide is 0.05-0.3L/min; the air flow rate is 0.7-10L/min, preferably 0.7-5L/min. The utility model discloses when discovering algae liquid pH value, carbon dioxide flow and air mass flow and satisfying above-mentioned condition simultaneously, the alga growth situation is good in the algae liquid.
Preferably, the bottom of the photobioreactor is provided with an aeration device, more preferably, the aeration device is a nano aeration pipe, and the nano aeration pipe is simple to install, convenient to replace and adjust and low in cost. The setting position of the nano aeration pipe is selected from the bottom in the closed reactor tank body or the lower support, preferably, the nano aeration pipe is uniformly distributed on the lower support, and the nano aeration pipe is uniformly spread by taking the waterproof LED lamp on the lower support as a positioning point or an isolation point, so that the aeration uniformity is improved.
The nano aeration pipe is connected with the first gas connector, the mixed gas of the sterile carbon dioxide and the sterile compressed air uniformly enters the algae liquid through the holes of the nano aeration pipe, and the disturbance of the algae liquid is promoted along with the rising of the bubbles, so that the growth of algae cells is in a suspension state. And regulating and controlling the carbon dioxide flow meter and the air flow meter to enable the algae liquid in the closed reactor tank to be in a flowing state, and keeping the regulation and control performance of the nutrient composition, the dissolved oxygen concentration and the pH value of the algae liquid, so that harmful bacteria breeding and carbon source shortage caused by algae accumulation are prevented, and finally, the death of algae is caused.
The photobioreactor also has the functions of liquid supply and liquid drainage, and the liquid supply function is to provide culture solution, make-up water or algae seed solution supplied by other reactors for the photobioreactor. The liquid discharge function is to discharge liquid such as algae liquid or cleaning liquid in the closed reactor tank body.
The photobioreactor still includes the culture solution joint, the culture solution connects culture solution case and the internal portion intercommunication of airtight reactor jar, for the photobioreactor provides the culture solution, satisfies the growth and the metabolism demand of alga in airtight reactor jar, and is preferred, set up the second valve between culture solution joint and the culture solution case, be convenient for control the supply of culture solution. Preferably, the culture solution connector is arranged on the surface cover.
Preferably, the photobioreactor further comprises a water supply joint for supplying pure water to the algae liquid, and the algae liquid density or nutrient substance density is adjusted according to different growth conditions of algae. The water supply joint is connected with the pure water tank, and a third valve is arranged between the pure water tank and the water supply joint, so that the supply of pure water can be conveniently controlled.
Preferably, the photobioreactor still includes raw materials algae seed joint for to add raw materials algae seed in the airtight reactor jar body, raw materials algae seed joint connection algae seed case, be equipped with the fourth valve between algae seed case and the raw materials algae seed joint, be convenient for control the supply of algae seed.
The photobioreactor also comprises a liquid discharge device for discharging algae liquid. The drainage device comprises a drainage connector, a multi-way pipe fitting, a sampling valve, a fifth valve and a sixth valve. The liquid discharge joint is arranged at the bottom of the closed reactor tank body and is connected with one opening of the multi-way pipe fitting, and other openings of the multi-way pipe fitting are respectively connected with the sampling valve, the fifth valve and the sixth valve through pipelines. The sampling valve is used for sampling in real time, monitoring the condition of algae in the growth process, judging the harvesting time or adjusting each parameter of the algae culture environment. The fifth valve is a harvesting valve and is connected with the algae treatment device through a pipeline, and finally, a valuable product is obtained. And the sixth valve is a drain valve and is connected with a sewer pipe through a pipeline for discharging waste or cleaning liquid.
Optionally, the sampling valve is separately arranged at the lower part or the bottom of the closed reactor tank body, so that an operator can sample at any time.
Preferably, the photobioreactor further comprises a cleaning device for cleaning the closed reactor tank. Cleaning device includes cleaning head and cleaning fluid joint, cleaning fluid joint and cleaning head are established respectively the surface and the internal surface of airtight reactor jar body face lid, cleaning fluid joint one end is connected airtight reactor jar external pure water case or disinfectant box, and the other end is connected the cleaning head of airtight reactor jar internal portion, preferably, be equipped with the seventh valve between pure water case or disinfectant box and the cleaning fluid joint, preferably, the cleaning head is the CIP cleaning head, provides 360 degrees powerful washability, will remaining stubborn algae or algae class metabolite washout in the airtight reactor jar is clean. And after a batch of algae is cultured, discharging the algae out of the closed reactor tank, opening a seventh valve, allowing clear water or disinfectant water in a pure water tank or a disinfectant tank to enter a cleaning fluid joint through a pipeline, finally spraying the clear water or the disinfectant water into the closed reactor tank from the cleaning head, cleaning residual algae or algae metabolites, and discharging all dirt and cleaning fluid from a sixth valve of the liquid discharging device.
Preferably, the closed reactor tank body also comprises a liquid level detection device, wherein the liquid level detection device comprises a pressure sensor and a capacitance sensor and is used for monitoring the height of the algae liquid in the closed reactor tank body. In a specific embodiment of the present invention, the bottom of the closed reactor tank is provided with a liquid level sensor for monitoring the height of the algae liquid in the closed reactor tank.
The internal multiple circuit and the pipeline of having of airtight reactor, for example, the circuit of waterproof LED lamp, the circuit of pH probe, the circuit of temperature probe, the gas circuit of carbon dioxide, the gas circuit of air, these circuit and pipeline connect out through a plurality of water joint respectively the airtight reactor jar body, corresponding equipment or air supply are connected respectively to the subdividing, and are preferred, be equipped with the spool box on the external wall of airtight reactor jar, the spool box is used for holding above-mentioned circuit and pipeline, protects above-mentioned circuit and pipeline, the operation of being convenient for.
The photobioreactor further comprises a control system for controlling the waterproof LED lamp, the temperature probe, the pH probe, the carbon dioxide flow meter and the air flow meter. Control system includes LED drive controller, PLC controlling means, pH controller, power transmitter, carbon dioxide flowmeter, air flow meter and control box, LED drive controller, PLC controlling means, pH controller and power transmitter establish the inside of control box, carbon dioxide flowmeter and air flow meter establish the inside or the outside at the control box. The LED driving controller, the pH controller, the power transmitter, the carbon dioxide flowmeter, the air flowmeter, the temperature probe and the liquid level detection device are connected with the PLC control device through lines and are controlled and coordinated by the PLC control device.
Specifically, the LED driving controller is connected with and controls the working time of the waterproof LED lamp and the energy of the emitted light, and the power transmitter is connected with and detects the power of the waterproof LED lamp.
The pH controller is connected with and controls the pH probe, the pH probe collects pH value data of the algae liquid and transmits the pH value data back to the pH controller and the PLC control device, the PLC control device controls the carbon dioxide flow meter to correspondingly increase or decrease the flow of carbon dioxide in a linkage manner according to the increase or decrease of the pH value, so that the pH value of the algae liquid returns to a normal level, and the pH value of the algae is continuously fed back to the pH controller and the PLC control device through the pH probe; meanwhile, the PLC control device reasonably controls the flow of the carbon dioxide according to the quantity of the raw material algae and the culture time of the algae, so as to ensure the sufficient supply of the carbon dioxide; on the other hand, the PLC control device correspondingly controls the air flow meter and adjusts the air flow according to the flow of the carbon dioxide, thereby ensuring the oxygen supply of the algae. The utility model discloses a PLC controlling means realizes multidimension degree, the harmony control to the various factors of alga growing environment to pH controller, pH probe, carbon dioxide flowmeter and air flow meter's coordinated control, provides suitable growing environment for the alga.
The temperature probe is connected with a PLC (programmable logic controller) through a line, the PLC is connected with and controls the supply of cooling water, namely, the water chiller is controlled, the temperature probe detects the temperature data of the algae liquid in real time and transmits the temperature data back to the PLC, the PLC controls the supply of the cooling water according to the temperature, and the cooling water takes away the water-proof LED lamp in the tank body of the closed reactor and the redundant heat generated by the life activities of the algae.
The liquid level detection device is connected with the PLC control device through a line, monitors and feeds back the liquid level height in the closed reactor tank body in real time, and plays a role in the processes of liquid supply, sampling, liquid drainage and cleaning.
Preferably, control system still includes the exhaust fan, does control system in time dispels the heat, the fretwork department in the side of control box is established to the exhaust fan.
Preferably, the outer surface of the control box can be further provided with a data display screen for displaying various parameters and operating conditions of the photobioreactor, such as temperature, pH value, carbon dioxide flow, air flow, liquid level height, and parameters of the number, working time, wavelength, power and the like of waterproof LED lamps, so that an operator can conveniently observe and control the photobioreactor.
Preferably, all joints of the present invention are quick joints.
The utility model provides a photobioreactor has solved current traditional little algae breeding mode and has received harmful effects problems such as weather, light, pollution, ambient temperature, has further solved little algae poor, the too slow problem of speed of growing stability in natural environment. By providing the microalgae with a spectrum which is pertinently suitable for photosynthesis and a good and stable culture environment, the growth speed of the microalgae can be greatly improved, the yield per unit area is high, the problems of influence of natural environmental factors on the microalgae, heavy metal pollution, fungal infection, competition of other algae and the like are greatly reduced, and the growth period of the microalgae is shortened. Adopt photobioreactor breed haematococcus pluvialis, can make the astaxanthin content in the haematococcus pluvialis to reach 4-6% of cell dry weight, and adopt traditional sunlight or fluorescent tube etc. to cultivate the astaxanthin content that haematococcus pluvialis generated and be less than 1.8-2.0% of cell dry weight at most. The photobioreactor can further solve the problem that a large amount of microalgae is dead due to cross infection of the large amount of chytrid or large-area pollution when the microalgae is cultured on a large scale through modularization, industrialization and standardization.
Preferably, the continuous culture reaction system comprises a disinfectant tank, a pure water tank, a product processing device and a main control computer, wherein the disinfectant tank is connected with the first photobioreactor and the second photobioreactor in parallel through a fifth pipeline, and the pure water tank is connected with the culture solution tank, the algae seed tank, the disinfectant tank, the first photobioreactor and the second photobioreactor in parallel through a sixth pipeline.
The algae seed box is used for preliminarily expanding and culturing algae seeds of algae, the culture solution box is used for storing and/or preparing culture solution required by the growth of the algae, the disinfectant box is used for storing and/or preparing disinfectant, and the pure water box is used for storing pure water.
The control system of the first photobioreactor and the second photobioreactor, the culture solution box, the algae seed box, the disinfection solution box and the pure water box are connected with a main control computer through circuits, and the main control computer controls the flow and time of liquid conveyed by each reactor and each material box. The second photobioreactor is connected to a product processing apparatus through a fourth pipeline, and the product processing apparatus is selected from a centrifuge, a dryer, a screening machine or a purification device.
The first photobioreactor has the function of culturing algae seeds and promoting the growth and the number increase of the algae seeds, and the second photobioreactor has the function of continuously culturing algae growing to a certain stage and promoting the accumulation of algae biomass to obtain valuable biomass. For example, in the culture of Haematococcus pluvialis, algae species grow in the first photobioreactor to promote the proliferation and growth of algae, and culture conditions are changed in the second photobioreactor to promote the accumulation of astaxanthin in the algae, thereby obtaining valuable astaxanthin.
Preferably, the number of the first photo-bioreactors is 2-100, the number of the first photo-bioreactors is reasonably adjusted according to the number and scale of algae cultivation, the drainage joints of the first photo-bioreactors are connected in parallel through the third pipeline and then connected to the third pump, the first photo-bioreactors are connected in parallel through the first pipeline to the algae liquid tank, the first photo-bioreactors are connected in parallel through the second pipeline to the culture liquid tank, the first photo-bioreactors are connected in parallel through the fifth pipeline to the disinfection liquid tank, and the first photo-bioreactors are connected in parallel through the sixth pipeline to the pure water tank.
Preferably, the number of the second photo-bioreactors is 2-100, the number of the second photo-bioreactors is reasonably adjusted according to the number and scale of algae cultivation, the drain connectors of the second photo-bioreactors are connected in parallel through the fourth pipeline and then connected to the fourth pump, the second photo-bioreactors are connected in parallel through the third pipeline to the first photo-bioreactor, the second photo-bioreactors are connected in parallel through the second pipeline to the culture solution tank, the second photo-bioreactors are connected in parallel through the fifth pipeline to the disinfection solution tank, and the second photo-bioreactors are connected in parallel through the sixth pipeline to the pure water tank.
The main control computer is internally provided with algae automatic culture program software, algae seed input quantity, algae liquid output quantity, culture liquid input quantity, disinfectant input quantity, pure water input quantity and product output quantity of the first photobioreactor and the second photobioreactor can be preset in advance, input time or input interval of algae seeds, culture liquid, disinfectant and pure water and output time or output interval of algae liquid and products can also be preset, and the algae seed box, the culture liquid box, the disinfectant box, the pure water box, the first photobioreactor and the second photobioreactor are controlled by the software and convey various feed liquid according to a preset program, so that the continuous culture reaction system can automatically and continuously run.
Drawings
Fig. 1 shows a whole perspective view of the photobioreactor according to the present invention.
Figure 2 shows a side perspective view of the photobioreactor according to the present invention.
Fig. 3 is a structural view showing a heat sink of the waterproof LED lamp 5.
Fig. 4 is a block diagram showing the circuit of the waterproof LED lamp 5.
Fig. 5 is a structural view showing the waterproof LED lamp 5.
FIG. 6 is a schematic diagram showing a structure of an algae continuous culture reaction system.
In the attached figure, 1-a pH probe, 2-a liquid level sensor, 3-a sampling valve, 4-a control box, 5-a waterproof LED lamp, 501-an exhaust pipe, 502-an end cap, 503-a transparent lampshade, 504-colorless transparent silicone oil, 505-a first LED chip, 506-a second LED chip, 507-a third LED chip, 508-a radiating seat, 509-a circuit, 510-a waterproof jacket, 511-a lamp sheet, 6-1-an upper bracket, 6-2-a lower bracket, 7-a condenser tube, 8-a CIP cleaning head, 9-a waterproof joint, 10-1-a gas joint, 10-2-a cleaning fluid joint, 10-3-a raw material algae seed joint, 10-4-a water supplementing joint and 10-5-a culture solution joint, 11-sight glass, 12-sterile filter, 13-fastener, 14-sealing ring, 15-1-carbon dioxide flowmeter, 15-2-air flowmeter, 16-pH controller, 17-power transmitter, 18-LED drive controller, 19-surface cover, 20-closed reactor tank, 21-flange, 22-PLC control device, 23-exhaust fan, 24-nano aeration pipe, 25-liquid discharge joint, 26-middle support, 27-wire tube box, 28-1-third valve, 28-2-fourth valve, 28-3-seventh valve, 28-4-second valve, 28-5-fifth valve, 28-6-sixth valve, 28-7-first valve, 29-temperature probe, 30-supporting foot, 31-main control computer, 32-pure water tank, 33-sixth pump, 34-disinfectant tank, 35-fifth pump, 36-culture solution tank, 37-second pump, 38-algae tank, 39-first pump, 40-first photobioreactor, 41-second photobioreactor, 42-centrifuge, 43-third pump, 44-fourth pump, 45-power supply line, 46-sixth pipeline, 47-fifth pipeline, 48-second pipeline, 49-first pipeline, 50-carbon dioxide gas pipe, 51-compressed air pipe, 52-first discharge branch, 53-second discharge branch, 54-third pipeline, 55-fourth pipeline, and 56-industrial control line.
Detailed Description
Example 1
The structure of the photobioreactor for algae cultivation in this embodiment is shown in fig. 1 and 2, the algae or algae cells in this embodiment are haematococcus pluvialis or haematococcus pluvialis cells, and the algae species is haematococcus pluvialis. The main body of the algae cultivation photobioreactor is a closed reactor tank body 20, the closed reactor tank body 20 is a vertical cylinder, the diameter is 100cm, the height is 225cm, the designed solution containing amount is 1000L, and the material of the closed reactor tank body 20 is stainless steel.
The top of the closed reactor tank body 20 is provided with a detachable surface cover 19, the surface cover 19 is provided with a gas joint 10-1, a cleaning liquid joint 10-2, a raw material algae seed joint 10-3, a water supplementing joint 10-4 and a culture solution joint 10-5, the surface cover 19 is connected and sealed with the closed reactor tank body 20 through a buckle 13, and a sealing ring 14 and a flange 21 are arranged between the surface cover 19 and the closed reactor tank body 20, so that the surface cover 19 and the closed reactor tank body 20 can be conveniently and fixedly connected, and the sealing performance of the photobioreactor is improved. The sight glass 11 is arranged on the outer wall of the closed reactor tank 20, so that the growth condition of algae in the closed reactor tank 20 can be observed conveniently.
The four supporting legs 30 are uniformly arranged on the periphery of the bottom of the closed reactor tank 20 and are used for supporting and stabilizing the closed reactor tank 20, and meanwhile, a space is reserved between the bottom of the closed reactor tank 20 and the ground, so that the algae liquid or products in the closed reactor tank 20 can be conveniently discharged.
The sealed reactor tank body 20 comprises 30 waterproof LED lamps 5, an upper support 6-1, a lower support 6-2 and a condenser pipe 7, the tops of the waterproof LED lamps 5 and the condenser pipe 7 are fixed on the upper support 6-1, the bottoms of the waterproof LED lamps are fixed on the lower support 6-2, the irradiation radius of the waterproof LED lamps is 10cm, the waterproof LED lamps are uniformly arranged, algae cells at all positions in the sealed reactor tank body 20 can receive enough light energy, and illumination dead angles caused by overlarge density of algae liquid or excessive algae are avoided.
The waterproof LED lamp 5 is structurally shown in FIGS. 3-5, the waterproof LED lamp 5 comprises an LED chip, a transparent lampshade 503, a heat dissipation component, an exhaust component and a waterproof device, and the waterproof device is mounted on the top of the transparent lampshade 503.
Waterproof LED lamp 5 is rectangular shape to vertical the placing in the middle part of airtight reactor jar body 20, transparent lamp shade 503 protects LED chip and circuit 509, keeps apart algae liquid and LED chip. The transparent lamp housing 503 has a long barrel shape and is sealed at the bottom and has an opening at the top, which allows the heat sink 508 and the circuit 509 to enter and exit the transparent lamp housing 503.
The waterproof device of the waterproof LED lamp 5 is an end cap 502, and the end cap 502 is disposed at the top opening of the transparent lamp housing 503 and seals the top opening of the transparent lamp housing 503, while allowing the circuit 509 of the LED chip to penetrate through the end cap 502. The upper end and the lower end of the end cap 502 are provided with openings, and the openings at the upper end and the lower end are locked by using a hose clamp and are positioned above the liquid level of the algae liquid.
The LED chip comprises a first LED chip 505, a second LED chip 506 and a third LED chip 507, wherein the wavelength of the first LED chip 505 is 390-420nm, the wavelength of the second LED chip 506 is 440-470nm, and the wavelength of the third LED chip 507 is 640-680 nm.
The heat dissipation part comprises colorless transparent silicone oil 504 and a heat dissipation seat 508, colorless transparent silicone oil 504 is filled in the transparent lampshade 503, the heat dissipation seat 508 and the LED chip are immersed in the colorless transparent silicone oil 504, the colorless transparent silicone oil 504 can absorb heat emitted when the LED chip emits light, the heat dissipation performance of the waterproof LED lamp 5 is improved, the waterproof LED lamp 5 can bear higher power or longer-time work, in addition, the colorless transparent silicone oil 504 has better transmission fidelity ratio to light than air, and the light emitted by the LED chip is not influenced.
The heat sink 508 is a long barrel shape with a square cross section, lamp pieces 511 are respectively arranged on the outer surfaces of the heat sink 508 facing different directions, and the LED chips are mounted on the lamp pieces 511. The LED chips are arranged in a row on the outer surface of the lamp sheet 511, and the row is repeated from top to bottom in the order of the first LED chip 505, the second LED chip 506, and the third LED chip 507. The LED chips on the same surface of the heat dissipation seat 508 are connected in series or in parallel, after the circuits 509 of the chips are converged, the chips enter the heat dissipation seat 508 from the opening at the bottom of the heat dissipation seat 508, then the end cap 502 extends out from the opening at the top of the heat dissipation seat 508, and then the chips are connected with an external power supply or control system, and the outside of the wire of the circuit 509 is sleeved with a waterproof jacket 510. The heat dissipation base 508 is made of metal aluminum, which is beneficial to heat dissipation of the LED chip.
The exhaust member is an exhaust pipe 501, and the exhaust pipe 501 is provided inside the waterproof jacket 510 and extends out of the transparent cover 503 together with the circuit 509. The exhaust pipe 501 communicates the inside and the outside of the transparent lamp enclosure 503, and is configured to guide the gas expanded by heat inside the transparent lamp enclosure 503 out of the waterproof LED lamp 5. Colorless transparent silicone oil 504 is heated the back volume expansion, and simultaneously, the inside air of transparent lamp shade 503 is heated and also can expand, and blast pipe 501 can be derived the unnecessary air in transparent lamp shade 503, prevents that transparent lamp shade 503 internal pressure is too big.
The circuitry 509 of the waterproof LED lamp 5 extends out of the closed reactor tank 20 through a waterproof joint and then connects to the LED drive controller 18.
The LED driving controller 18 controls the power of the LED chips to be increased or decreased simultaneously, so that the energy of the LED chips is controlled, and different requirements of algae cultivation on light energy can be met. The LED driving controller 18 can control the power of the first LED chip 505, the second LED chip 506, and the third LED chip 507 to be simultaneously increased or decreased. When the power is zero, the light of the LED chip is extinguished.
The light quantum flux density of the waterproof LED lamp 5 is 6 × 105μmol/m2S, the working time of the waterproof LED lamp 5 is a culture period for the rapid growth and proliferation of the algae cells, the culture period is 60 hours, and the power is 300W. A power transmitter may be used for measuring the power of the watertight LED lamp 5.
The support is made of stainless steel and comprises an upper support 6-1, a lower support 6-2 and a middle support 26, the upper support 6-1 and the lower support 6-2 are provided with criss-cross crossbeams, a clamping ring is arranged at the intersection of the two crossbeams, and the clamping ring is used for being sleeved with the waterproof LED lamp 5. The middle bracket 26 is a longitudinal support bar fixed between the upper bracket 6-1 and the lower bracket 6-2, and is used for fixing the condensation duct 7.
The closed reactor tank 20 internally comprises five condensation pipes 7, the condensation pipes 7 are spiral and are sequentially arranged at the upper part, the middle part and the lower part of the closed reactor tank 20 from top to bottom, and all the condensation pipes 7 are immersed below the liquid level of the algae liquid. The cooling water is introduced into the condensation pipe 7, and a large amount of heat emitted by the waterproof LED lamp 5 and redundant heat released in the algae growth and metabolism process are taken out of the closed reactor tank body 20, so that the temperature inside the closed reactor tank body 20 is always kept in a temperature range suitable for algae growth and metabolism. The condenser tube 7 adjusts the temperature inside the closed reactor tank 20 to 25 ℃. The inlets and outlets of the 5 condenser pipes 7 connected in series are respectively connected with a cold water inlet pipe and a cold water outlet pipe, the cold water inlet pipe and the cold water outlet pipe are connected to a cooling water connector at the upper part of the outer wall of the closed reactor tank body 20, water is supplied by the cooling water connectors uniformly, and the cooling water connectors are connected to a water chiller through pipelines and first valves 28-7.
A temperature probe 29 is arranged in the sealed reactor tank 20, the temperature probe 29 is immersed below the liquid level of algae liquid, a circuit of the temperature probe 29 extends out of the sealed reactor tank 20 through a waterproof connector 9 and then is connected with a PLC (programmable logic controller) 22, the PLC 22 is connected with a water chiller, when the temperature in the sealed reactor tank 20 is high, the PLC 22 starts the water chiller, the movement of cooling water in a condenser pipe 7 brings away redundant heat, and when the temperature in the sealed reactor tank 20 is low, the PLC 22 suspends the water chiller. The temperature probe 29 is combined with the multi-dimensional adjustable lighting conditions provided by the waterproof LED lamp 5 to provide a suitable growing environment for Haematococcus pluvialis, and promote the growth of Haematococcus pluvialis and the accumulation of astaxanthin.
The photobioreactor further comprises a gas supply device for supplying carbon dioxide and air required for algae growth to the photobioreactor. The gas supply device comprises a sterile filter 12, a carbon dioxide flowmeter 15-1, an air flowmeter 15-2, a gas connector 10-1 and a second gas connector, one end of the carbon dioxide flowmeter 15-1 and one end of the air flowmeter 15-2 are connected with a carbon dioxide gas source and an air gas source, the other end of the carbon dioxide flowmeter 15-1 and the air flowmeter 15-2 are connected with a first gas connector, the gas connector 10-1 serves as a gas outlet, the gas connector 10-1 is arranged on a surface cover of the closed reactor tank body, and the first gas connector is arranged at the middle upper part of the outer wall and communicated with.
The carbon dioxide gas source is industrial carbon dioxide, the air gas source is compressed air formed by air passing through an air compressor, and the carbon dioxide is used as a carbon source required by the growth of algae. When the device is used, carbon dioxide and air control flow through the carbon dioxide flow meter 15-1 and the air flow meter 15-2, then enter the closed reactor tank 20 through the first gas connector, and flow out of the closed reactor tank 20 from the gas connector 10-1 after the carbon dioxide and the air are subjected to algae growth and metabolism utilization.
A pH probe 1 is arranged at the middle lower part of a closed reactor tank body 20, the pH probe 1 is immersed in algae liquid and is connected with a pH controller 16 outside the closed reactor tank body 20 through a line and a waterproof joint 9, and the pH controller 16 is connected with a carbon dioxide flowmeter 15-1 and an air flowmeter 15-2 through a PLC (programmable logic controller) 22. The pH probe 1 is used for monitoring whether the pH value of the algae liquid is suitable for the growth of algae in real time, the algae can generate various nutrients and excrement in the growth and metabolism process, the substances are partially released into the algae liquid and can change the pH value of the algae liquid, and the pH value of the algae liquid is a key factor influencing the growth and metabolism of the algae.
The pH value of the algae liquid in the embodiment is controlled to be 6.8, which is beneficial to the growth of algae, and meanwhile, the flow rate of carbon dioxide is 0.3L/min, and the flow rate of air is 5L/min. The utility model combines the pH value of the algae liquid with the carbon source carbon dioxide required by the growth of the algae to regulate and control. Because carbon dioxide dissolves in behind the algae liquid, the algae liquid shows acidity, and pH value reduces, the utility model discloses establish the contact of carbon dioxide and pH value from this, through real-time supervision algae liquid pH value, linkage adjustment carbon dioxide flow, and then reach the dual purpose of control algae carbon source supply volume and algae liquid pH value simultaneously.
The photobioreactor also comprises an aeration device which is a nano aeration pipe 24, and the nano aeration pipe 24 is simple to install, convenient to replace and adjust and low in cost. The nano aeration pipes 24 are uniformly distributed on the lower support 6-2, and the nano aeration pipes 24 are uniformly spread by using the waterproof LED lamps 5 on the lower support 6-2 as positioning points or isolation points, so that the aeration uniformity is improved.
The nano aeration pipe 24 is connected with the gas connector 10-1, and the mixed gas of sterile carbon dioxide and sterile compressed air uniformly enters the algae liquid through the holes of the nano aeration pipe 24, and the disturbance of the algae liquid is promoted along with the rising of bubbles, so that the growth of algae cells is in a suspension state, the regulation and control performance of the nutrient composition, the dissolved oxygen concentration and the pH value of the algae liquid are maintained, and the algae is prevented from being accumulated to cause the breeding of harmful bacteria and the lack of a carbon source, and finally the death of the algae is caused.
The photobioreactor also has the functions of liquid supply and liquid drainage, and the liquid supply function is to provide culture solution, make-up water or algae seed solution supplied by other reactors for the photobioreactor. The liquid discharge function is to discharge the algae liquid, the cleaning liquid and other liquids in the photobioreactor.
The culture solution connector 10-5 is used for communicating the culture solution box with the inside of the closed reactor tank body 20 to provide culture solution for the photobioreactor, so that the growth and metabolism requirements of algae are met, and a second valve 28-4 is arranged between the culture solution connector 10-5 and the culture solution box, so that the supply of the culture solution is convenient to control. The water supply joint 10-4 is used for supplying pure water to the algae liquid and adjusting the density of the algae liquid or the density of nutrient substances according to different growth conditions of algae. The water supply joint 10-4 is connected with a pure water tank 32, and a third valve 28-1 is arranged between the pure water tank 32 and the water supply joint 10-4, so that the supply of pure water is convenient to control. The raw material algae seed connector 10-3 is used for adding raw material algae seeds into the photobioreactor, the raw material algae seed connector is connected with the algae seed box 38, and a fourth valve 28-2 is arranged between the algae seed box 38 and the raw material algae seed connector 10-3, so that the supply of the algae seeds is convenient to control.
The drainage means comprises a drainage connector 25, a tee fitting, a sample valve 3, a fifth valve 28-5 and a sixth valve 28-6. The liquid discharge joint 25 is arranged at the bottom of the closed reactor tank 20 and is connected with one opening of the tee pipe, and the other openings of the tee pipe are respectively connected with the fifth valve 28-5 and the sixth valve 28-6 through pipelines. The sampling valve 3 is independently arranged at the lower part of the closed reactor tank body 20, so that an operator can sample at any time, the condition of the algae liquid in the growth process is monitored, and the harvesting time is judged or all parameters of the algae culture environment are adjusted. The fifth valve 28-5 is a harvest valve and is connected to the algae processing plant via a pipe to finally obtain a valuable product. The sixth valve 28-6 is a drain valve and is connected to a sewer pipe through a pipe for discharging waste or cleaning solution.
The photobioreactor further comprises a cleaning device for cleaning the closed reactor tank 20. The cleaning device comprises a CIP cleaning head 8 and a cleaning liquid joint 10-2, the cleaning liquid joint 10-2 and the CIP cleaning head 8 are respectively arranged on the outer surface and the inner surface of the surface cover of the closed reactor tank body 20, one end of the cleaning liquid joint 10-2 is connected with a disinfectant tank 34 outside the closed reactor tank body 20, the other end of the cleaning liquid joint is connected with the CIP cleaning head 8, and a seventh valve 28-3 is arranged between the disinfectant tank 34 and the cleaning liquid joint 10-2. After a batch of algae is cultured, the algae is discharged out of the closed reactor tank 20, then the seventh valve 28-3 is opened, the disinfectant in the disinfectant tank 34 enters the cleaning fluid connector 10-2 through a pipeline, and finally the disinfectant is sprayed into the closed reactor tank 20 from the CIP cleaning head 8, the residual algae or algae metabolites are cleaned, and all dirt and cleaning fluid are discharged from the sixth valve 28-6.
The bottom of the closed reactor tank 20 is provided with a liquid level sensor 2, and is connected with a PLC control device 22 through a line, the liquid level height in the closed reactor tank 20 is monitored and fed back in real time, and the liquid level sensor plays a role in the processes of liquid supply, sampling, liquid drainage and cleaning.
The circuit of the waterproof LED lamp 5, the circuit of the pH probe 1, the circuit of the temperature probe 29, the gas circuit of carbon dioxide and the gas circuit of air in the closed reactor tank 20 are respectively connected out of the closed reactor tank 20 through a plurality of waterproof joints 9, a line box 27 is arranged on the outer wall of the closed reactor tank 20, the line box 27 is used for accommodating the lines and the pipelines, and then the lines and the pipelines are respectively connected with corresponding equipment or gas sources to protect the lines and the pipelines and facilitate operation.
The photobioreactor further comprises a control system for controlling the waterproof LED lamp 5, the temperature probe 29, the pH probe 1, the carbon dioxide flow meter 15-1 and the air flow meter 15-2. The control system comprises an LED drive controller 18, a PLC control device 22, a pH controller 16, a power transmitter 17, a carbon dioxide flowmeter 15-1, an air flowmeter 15-2 and a control box 4, wherein the LED drive controller 18, the PLC control device 22, the pH controller 16 and the power transmitter 17 are arranged inside the control box 4, and the carbon dioxide flowmeter 15-1 and the air flowmeter 15-2 are arranged on the outer side of the control box 4. The LED drive controller 18, the pH controller 16, the power transmitter 17, the carbon dioxide flow meter 15-1, the air flow meter 15-2, the temperature probe 29 and the liquid level sensor 2 are connected with the PLC control device 22 through lines and are controlled and coordinated by the PLC control device 22. The side of the control box 4 is provided with a hollow-out fan 23 for timely heat dissipation of the control system. The outer surface of the control box 4 is also provided with a data display screen for displaying all parameters and operation conditions of the photobioreactor, so that an operator can observe and control the photobioreactor conveniently.
The pH controller 16 is connected with and controls the pH probe 1, the pH probe 1 collects pH value data of the algae liquid and transmits the pH value data back to the pH controller 16 and the PLC control device 22, the PLC control device 22 controls the carbon dioxide flowmeter 15-1 in a linkage manner to correspondingly increase or reduce the flow of carbon dioxide according to the increase or decrease of the pH value, so that the pH value of the algae liquid returns to a normal level, and the pH value of the algae is continuously fed back to the pH controller 16 and the PLC control device 22 through the pH probe 1; meanwhile, the PLC control device 22 reasonably controls the flow of the carbon dioxide according to the quantity of the raw material algae and the culture time of the algae, so as to ensure the sufficient supply of the carbon dioxide; on the other hand, the PLC control device 22 controls the air flow meter 15-2 accordingly according to the flow rate of the carbon dioxide, and adjusts the air flow rate to ensure the oxygen supply of the algae.
Temperature probe 29 passes through line connection PLC controlling means 22, and PLC controlling means 22 connects and control the supply of cooling water, controls cold water machine promptly, and temperature probe 29 real-time detection and gather algae liquid temperature data to pass PLC controlling means 22 back, and PLC controlling means 22 is according to the supply of the high or low control cooling water of temperature, and the cooling water takes away the interior waterproof LED lamp 5 of airtight reactor jar body and the unnecessary heat that algae life activity produced.
Example 2
The structures of the first photo-bioreactor and the second photo-bioreactor of the embodiment are the same as those of the photo-bioreactor of the embodiment 1, and the structures of the first light supplement lamp and the second light supplement lamp are the same as those of the waterproof LED lamp of the embodiment 1.
As shown in FIG. 6, the continuous algae culture system of this embodiment comprises three first photo-bioreactors 40 and three second photo-bioreactors 41, and the algal species tank 38, the culture solution tank 36, the disinfectant tank 34, and the purified water tank 32 are connected in parallel to the three first photo-bioreactors 40 and the three second photo-bioreactors 41, respectively. The carbon dioxide air pipe 50 and the compressed air pipe 51 are connected into the three first photo-bioreactors 40 and the three second photo-bioreactors 41 in parallel, so as to provide carbon sources and oxygen for the growth and metabolism of algae. The algae of this example is Haematococcus pluvialis, and the algal species is Haematococcus pluvialis.
The algae box 38 is used for preliminary expanding and culturing algae seeds, also called a raw material box, and provides the algae seeds for the first photobioreactor 40. Algae seed case 38 includes first agitator, first aseptic filter and first pump 39, and the motor of first agitator is established at the top of algae seed case 38, and inside the stirring rake extended into algae seed case 38, the algae liquid stirring that contains the algae kind with inside, prevent that the algae kind accumulation from deposiing and causing a large amount of deaths, improve the activity of algae kind, and first agitator and first pump 39 pass through industrial control circuit 56 and connect main control computer 31, the opening and closing of the flow of automatic control first pump 39 and first agitator.
The first sterile filter is arranged at the top of the algae box 38, and has the functions of filtering and sterilizing the air entering and exiting the algae box 38, so that a sterile environment is provided for the algae, and the algae is ensured not to be polluted from the source.
The bottom of the algae seed box 38 is provided with a discharge hole and is connected with a first pipeline 49, and the top of the algae seed box 38 is provided with a feed hole for adding algae seeds and algae liquid into the algae seed box 38.
After the first pipeline 49 is connected with the discharge port of the algae seed box 38, the first pump 39 is connected, and then the raw material algae seed connectors 10-3 of the three first photo-bioreactors 40 are respectively connected in parallel, so that the states of the input algae seeds of each first photo-bioreactor 40 are the same, and the three first photo-bioreactors 40 are conveniently and uniformly controlled and managed.
The culture solution box 36 is used for storing and configuring culture solution required by algae growth, the culture solution box 36 comprises a second stirrer, a second sterile filter and a second pump 37, a motor of the second stirrer is arranged at the top of the culture solution box 36, a stirring paddle extends into the culture solution box 36 to uniformly stir the culture solution in the culture solution box, the second stirrer and the second pump 37 are connected with the main control computer 31 through an industrial control line 56, and the flow of the second pump 37 and the opening and closing of the second stirrer are automatically controlled.
The second sterile filter is arranged at the top of the culture solution box 36, and has a filtering and sterilizing effect on air entering and exiting the culture solution box 36, so that the possibility that the culture solution is polluted by external bacteria or microorganisms is reduced as much as possible.
The bottom of the culture solution box 36 is provided with a discharge hole and is connected with a second pipeline 48, and the top of the culture solution box 36 is provided with a feed inlet for adding culture solution into the culture solution box 36.
The second pipe 48 is connected to the discharge port of the culture solution tank 36, and then connected to the second pump 37, and then connected to the three first photo-bioreactors 40 and the three second photo-bioreactors 41 in parallel, respectively, so as to ensure that the sterile culture solution input by each first photo-bioreactor 40 is the same, and the culture solution input by each second photo-bioreactor 41 is the same, thereby facilitating the unified control and management of the three first photo-bioreactors 40 and the three second photo-bioreactors 41.
The outer side of the first photobioreactor 40 is provided with a third pump 43, the raw material algae seed connector 10-3 of the first photobioreactor 40 is connected with the algae seed box 38 through a first pipeline 49, the culture solution connector 10-5 is connected with the culture solution box 36 through a second pipeline 48, the fifth valve 28-5 is connected with the raw material algae seed connector 10-3 of the second photobioreactor 41 through a third pipeline 54, the third pump 43 is connected into the third pipeline 54, and the third pump 43 is connected with the main control computer 31 through an industrial control circuit 56. The first photo bioreactor 40 is provided with a first light supplement lamp therein to provide light energy for the growth of algae. The control system of the first photobioreactor 40 is manually controlled, and an operator adjusts the carbon dioxide flow meter to increase or decrease the carbon dioxide supply according to the increase or decrease of the pH value and adjusts the increase or decrease of the cooling water flow according to the increase or decrease of the temperature.
The carbon dioxide gas pipe 50 and the compressed air pipe 51 are respectively connected with the carbon dioxide flow meter 15-1 and the air flow meter 15-2 of the first photobioreactor 40.
After the sixth valves 28-6 of the three first photobioreactors 40 are connected in parallel, the wastewater waste in the first photobioreactors 40 is discharged into a sewer pipe or a waste liquid storage tank from the first drainage pipe branch 52; after the fifth valves 28-5 of the three first photo-bioreactors 40 are connected in parallel, the third pipeline 54 is connected, and then the third pump 43 is connected, and the three second photo-bioreactors 41 are correspondingly input.
The top of the first photobioreactor 40 is further provided with a cleaning liquid joint 10-2 and a water supplementing joint 10-4, the cleaning liquid joint 10-2 is connected with the disinfectant tank 34 through a fifth pipeline 47, and the water supplementing joint 10-4 is connected with the pure water tank 32 through a sixth pipeline 46.
The fourth valve 28-2 is arranged on the first pipeline 49 in front of the raw material algae seed joint 10-3, the second valve 28-4 is arranged on the second pipeline 48 in front of the culture solution joint 10-5, the seventh valve 28-3 is arranged on the fifth pipeline 47 in front of the cleaning solution joint 10-2, the third valve 28-1 is arranged on the sixth pipeline 46 in front of the water supply joint 10-4, and the sixth valve 28-6 is arranged in front of the first water discharge pipeline branch 52. The opening or closing of the valves can control the flow or stop of the feed liquid at the inlets or outlets, respectively, so as to temporarily activate or deactivate a certain first photobioreactor 40.
When the device is used, algae seeds in the algae seed box 38 are input into the first photobioreactor 40 through the first pipeline 49, the first pump 39 and the raw material algae seed connector 10-3, the culture solution in the culture solution box 36 is input into the first photobioreactor 40 through the second pipeline 48, the second pump 37 and the culture solution connector 10-5, the algae seeds grow in the sterile environment of the culture solution, and the control system of the first photobioreactor 40 controls the temperature and the pH value in the first photobioreactor 40 and inputs carbon dioxide and air for the growth and metabolism of the algae. When the concentration of the algae or the culture solution in the first photobioreactor 40 is too high, pure water in the pure water tank 32 is input into the first photobioreactor 40 through the sixth pipeline 46, the sixth pump 33, and the makeup water connection 10-4. After the algae has grown to a certain stage, it is necessary to enter a biomass accumulation stage to obtain valuable products. From the fifth valve 28-5, the algae is fed via the third pipe 54 and the third pump 43 to the second photobioreactor 41 for cultivation in the biomass accumulation stage. Finally, the sterilizing liquid of the sterilizing liquid tank 34 is inputted through the fifth pipe 47, the fifth pump 35 and the cleaning liquid joint 10-2 and cleans the first photo bioreactor 40, and then all the waste liquid is discharged through the first water discharge pipe branch 52, and the first photo bioreactor 40 waits for the next round of algal cultivation.
The fourth pump 44 is disposed outside the second photobioreactor 41, and the raw material algal species connector 10-3 of the second photobioreactor 41 is connected to the first photobioreactor 40 through the third pipeline 54, that is, the algae cultured in the first photobioreactor 40 is distributed to different second photobioreactors 41 through the third pipeline 54. The culture solution connector 10-5 is connected with the second pipeline 48, the fifth valve 28-5 is connected with the inlet of the product processing device through a fourth pipeline 55, the product processing device is a centrifuge 42, a fourth pump 44 is connected to the fourth pipeline 55, and the control system of the second photobioreactor 41 controls the temperature, the pH value, the carbon dioxide and the air supply of the second photobioreactor 41. The fourth pump 44 is connected to the main control computer 31 via an industrial control line 56. A second light supplement lamp is arranged inside the second photobioreactor 41 to provide light energy for the growth and metabolism of algae.
The branches of the carbon dioxide air pipe 54 and the compressed air pipe 55 respectively pass through a carbon dioxide flow meter and an air flow meter of the second photobioreactor 41 and then enter the second photobioreactor 41. The control system of the second photobioreactor 41 is manually controlled, and the operator adjusts the carbon dioxide flow meter to increase or decrease the carbon dioxide supply according to the increase or decrease of the pH value, and adjusts the increase or decrease of the cooling water flow according to the increase or decrease of the temperature.
The sixth valves 28-6 of the three second photo bioreactors 41 are connected in parallel and then connected to the second water drain pipeline branch 53, so that the waste water and waste in the second photo bioreactors 41 can be discharged into the sewer pipeline or the waste liquid storage tank.
The other connections and valve connections of the second photobioreactor 41 are the same as those of the first photobioreactor 40.
The disinfectant tank 34 is used for storing and/or preparing disinfectant, the disinfectant tank 34 comprises a third stirrer, a fifth sterile filter and a fifth pump 35, a motor of the third stirrer is arranged at the top of the disinfectant tank 34, a stirring paddle extends into the disinfectant tank 34 to uniformly stir the internal disinfectant, the third stirrer and the fifth pump 35 are connected with the main control computer 31 through an industrial control circuit 56, and the flow of the fifth pump 35 and the opening and closing of the third stirrer are automatically controlled.
The fifth sterile filter is arranged at the top of the disinfectant tank 34, and has a filtering and sterilizing effect on air entering and exiting the disinfectant tank, so that the possibility that the disinfectant is polluted by external bacteria or microorganisms is reduced as much as possible.
A liquid outlet is arranged at the bottom of the disinfectant tank 34 and connected with a fifth pipeline 47, and a liquid feeding port is arranged at the top of the disinfectant tank 34 and used for feeding disinfectant into the disinfectant tank 34.
The fifth pipeline 47 is connected to the discharge port of the disinfectant tank 34, and then connected to the fifth pump 35, and then connected to the three first photo-bioreactors 40 and the three second photo-bioreactors 41 in parallel, respectively, so as to ensure that the disinfectant input by each first photo-bioreactor 40 is the same, and the disinfectant input by each second photo-bioreactor 41 is the same, thereby facilitating the unified control and management of the three first photo-bioreactors 40 and the three second photo-bioreactors 41.
The pure water tank 32 is used for storing pure water, the pure water tank 32 comprises a sixth sterile filter and a sixth pump 33, the sixth pump 33 is connected with the main control computer 31 through an industrial control circuit 56, and the flow of the sixth pump 33 is automatically controlled.
The sixth sterile filter is arranged on the top of the pure water tank 32, and has a filtering and sterilizing effect on the air entering and exiting the pure water tank 32, so that the possibility that the pure water is polluted by external bacteria or microorganisms is reduced as much as possible. The bottom of the pure water tank 32 is provided with a water outlet and is connected with a sixth pipeline 46, the top of the pure water tank 32 is provided with a water filling port for adding pure water into the pure water tank 32, and the water filling port is connected with a pure water machine through a pipeline. The sixth pump 33 is connected to a sixth pipe 46 to power the pure water supply. The sixth pipeline 46 is connected to the outlet of the pure water tank 32, and then connected to the sixth pump 33, and further connected to the three first photo-bioreactors 40, the three second photo-bioreactors 41, the algae seed tank 38, the culture solution tank 36, and the disinfectant tank 34 in parallel.
The first photobioreactor 40, the second photobioreactor 41, the culture solution tank 36, the algae seed tank 38, the disinfectant tank 34 and the pure water tank 32 are connected with the main control computer 31 through circuits, and the main control computer 31 controls the flow and time of liquid conveyed by each reactor and each material tank. Specifically, the main control computer 31 is provided with an automatic algae cultivation program software, which can preset the algae seed input amount, the culture solution input amount, the disinfectant input amount, the pure water input amount and the algae output amount of the first photobioreactor 40 in advance, and can also preset input time or input interval of algae seeds, culture solution, disinfectant and pure water, and output time or output interval of algae; the algae automatic breeding program software can preset the algae input quantity, the product output quantity, the culture solution input quantity, the disinfectant input quantity and the pure water input quantity of the second photobioreactor 41 in advance, and can also preset the algae, the culture solution, the disinfectant and the pure water input time or input interval and the product output time or output interval. The algae seed box 38, the culture solution box 36, the disinfectant box 34, the pure water box 32, the first photobioreactor 40 and the second photobioreactor 41 are controlled by software and convey various feed liquids according to a preset program, so that the continuous culture reaction system automatically and continuously operates.
The continuous culture reaction system comprises a power supply line 45 for supplying electric energy to all electric equipment of the system, and the power supply line 45 is connected with a culture solution tank 36, an algae seed tank 38, a disinfectant tank 34, a pure water tank 32, a first photobioreactor 40, a second photobioreactor 41, a main control computer 31, a centrifuge 42, a first pump 39, a second pump 37, a third pump 43, a fourth pump 44, a fifth pump 35, a sixth pump 33, a first stirrer, a second stirrer, a third stirrer and a control system.

Claims (10)

1. The continuous algae culture reaction system is characterized by comprising at least two photobioreactors, a culture solution box and an algae seed box, wherein the two photobioreactors are respectively a first photobioreactor and a second photobioreactor, the algae seed box is connected with the first photobioreactor through a first pipeline and provides algae solution containing algae seeds for the first photobioreactor, the culture solution box is connected with the first photobioreactor and the second photobioreactor in parallel through a second pipeline and provides nutrient materials for different growth stages of algae, and the first photobioreactor is connected with the second photobioreactor through a third pipeline;
the photobioreactor includes the airtight reactor jar body, the internal portion of airtight reactor jar is equipped with waterproof LED lamp, and the external portion of airtight reactor jar is equipped with LED drive controller, waterproof LED lamp includes LED chip, transparent lamp shade, radiating part, exhaust part and watertight fittings, the surface at the radiating seat of radiating part is established to the LED chip, and LED chip and radiating part establish inside transparent lamp shade, watertight fittings establishes the top at transparent lamp shade, the LED chip can send the light that is suitable for the multiple wavelength range of algae growth, the energy of LED drive controller control LED chip increases simultaneously or reduces.
2. The continuous culture reaction system according to claim 1, wherein the plurality of wavelength ranges are selected from wavelength ranges of two or more combinations of: 390-420nm, 440-470nm or 640-680 nm.
3. The continuous culture reaction system of claim 2, wherein the LED chips comprise a first LED chip, a second LED chip and a third LED chip, the wavelength of the light emitted by the first LED chip is 390-420nm, the wavelength of the light emitted by the second LED chip is 440-470nm, and the wavelength of the light emitted by the third LED chip is 640-680 nm.
4. The continuous culture reaction system according to claim 1, wherein the heat dissipation member comprises colorless transparent silicone oil and a heat dissipation base, and the colorless transparent silicone oil is filled in the transparent lamp cover to conduct heat dissipated from the LED chip out of the transparent lamp cover.
5. The continuous culture reaction system of claim 4, wherein the heat sink has an elongated barrel shape, the heat sink has a polygonal cross-section, and the LED chips are mounted in rows on the outer surface of the heat sink facing different directions.
6. The continuous culture reaction system of claim 1, wherein the exhaust component is communicated with the inside and the outside of the transparent lampshade and is used for guiding the gas expanded by heat inside the transparent lampshade out of the waterproof LED lamp.
7. The continuous culture reaction system of claim 1, wherein the LED drive controller controls the power of the LED chips to be increased or decreased simultaneously.
8. The continuous culture reaction system according to claim 1, wherein at least one pH probe is arranged in the closed reactor tank, the pH probe is connected with a pH controller outside the closed reactor tank through a line, the pH controller is connected with a carbon dioxide flowmeter and an air flowmeter through a PLC (programmable logic controller) control device, and the pH controller and the PLC control system control the flow rate of carbon dioxide supplied to the closed reactor tank to increase or decrease according to the increase or decrease of the pH value detected by the pH probe.
9. The continuous culture reaction system according to claim 8, wherein the inside of the closed reactor tank comprises at least one condensation pipe, the shape of the condensation pipe is selected from one or a combination of two or more of a straight line shape, a U-shape, a spiral shape or a serpentine shape, and the condensation pipe is arranged at one or more positions of the upper part, the middle part and the lower part of the closed reactor tank;
the inlet and the outlet of the condenser pipe are respectively connected with a cold water inlet pipe and a cold water outlet pipe, and the cold water inlet pipe and the cold water outlet pipe are connected with a water chiller outside the tank body of the closed reactor;
at least one temperature probe is arranged in the closed reactor tank body and connected with the PLC control device through a line, and the PLC control device is connected with the water chiller.
10. The continuous culture reaction system of claim 1, comprising a disinfectant tank, a purified water tank, a product processing device and a main control computer, wherein the disinfectant tank is connected in parallel with the first photobioreactor and the second photobioreactor through a fifth pipeline, the purified water tank is connected in parallel with the culture tank, the algal seed tank, the disinfectant tank, the first photobioreactor and the second photobioreactor through a sixth pipeline, and the disinfectant tank and the purified water tank are connected in circuit with the main control computer;
the second photobioreactor is connected with a product processing device through a fourth pipeline, the first photobioreactor, the second photobioreactor, the culture solution box, the algae seed box, the disinfectant box and the pure water box are connected with a main control computer through circuits, and the main control computer controls the flow and time of liquid conveying of each reactor and each material box.
CN201921919889.8U 2019-11-08 2019-11-08 Algae continuous culture reaction system Active CN211339537U (en)

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Application Number Priority Date Filing Date Title
CN201921919889.8U CN211339537U (en) 2019-11-08 2019-11-08 Algae continuous culture reaction system

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