CN211328940U

CN211328940U - Special ultrafiltration device of exosome extraction separation

Info

Publication number: CN211328940U
Application number: CN201921990874.0U
Authority: CN
Inventors: 陈锦阳; 乔博超; 李静静
Original assignee: Zhejiang Healthfuture Biomedical Technology Co ltd
Current assignee: Zhejiang Healthfuture Biomedical Technology Co ltd
Priority date: 2019-11-18
Filing date: 2019-11-18
Publication date: 2020-08-25
Anticipated expiration: 2029-11-18

Abstract

The utility model relates to the technical field of exosome extraction and separation equipment, in particular to an ultrafiltration device special for exosome extraction and separation, which comprises a bracket, wherein an installation cavity is arranged at the front side of the bracket; a hollow liquid storage tank, an ultrafiltration assembly and a hollow collecting tank are sequentially arranged in the mounting cavity from top to bottom; this special ultrafiltration device of exosome extraction separation is through the negative pressure mechanism that is equipped with, drive piston motion under the effect of cylinder piston pole promptly, and then form the atmospheric pressure difference between liquid storage pot and collection tank, thereby make the exosome extract the separation through the milipore filter under the effect of atmospheric pressure difference, this design has changed traditional milipore filter and has extracted the mode of exosome through long-time low-speed centrifugation, on the one hand, the time of extracting the separation has been reduced, on the other hand, the efficiency of extracting the separation has been improved, the problem that there is the drawback in the mode of traditional milipore filter extraction exosome has been solved.

Description

Special ultrafiltration device of exosome extraction separation

Technical Field

The utility model relates to an exosome draws splitter technical field, specifically is a special ultrafiltration device of exosome extraction separation.

Background

The expression level of the content in the exosome is closely related to the type of cells and the health pathological state of the cells, and the exosome also has huge functions in the aspects of intercellular information communication and disease diagnosis;

when the existing ultrafiltration equipment is used for extracting and separating exosomes, the exosomes are extracted and separated through an ultrafiltration membrane through long-time low-speed centrifugation, however, the method has the defects of long extraction time and low extraction efficiency, and in view of the above, the special ultrafiltration device for extracting and separating exosomes is provided.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a special ultrafiltration device of exosome extraction separation to solve the problem that there is the drawback in the mode that the exosome was extracted to the traditional milipore filter that provides in the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme:

an ultrafiltration device special for exosome extraction and separation comprises a bracket, wherein a mounting cavity is arranged on the front side of the bracket; a hollow liquid storage tank, an ultrafiltration assembly and a hollow collecting tank are sequentially arranged in the mounting cavity from top to bottom, and a negative pressure mechanism is arranged on the collecting tank; the liquid storage tank comprises a liquid inlet pipe, the liquid inlet pipe is communicated with the liquid storage tank, the liquid inlet pipe penetrates through the top surface of the support and protrudes above the support, and a first valve is arranged on the liquid inlet pipe; the ultrafiltration assembly comprises a liquid inlet hopper, the liquid inlet hopper is communicated with the liquid storage tank, a connecting pipe is arranged below the liquid inlet hopper, the liquid inlet hopper is communicated with the connecting pipe, the liquid inlet hopper and the connecting pipe are of an integrally formed structure, a ultrafiltration membrane is embedded in the position, close to the bottom end, of the inner wall of the connecting pipe, an extraction pipe is arranged on the connecting pipe, the extraction pipe is communicated with the connecting pipe, and a second valve is arranged on the extraction pipe; the collecting tank comprises a collecting pipe, the two ends of the collecting pipe are respectively communicated with the connecting pipe and the inside of the collecting tank, a third valve is arranged on the collecting pipe, a liquid discharge pipe is arranged on the position, close to the bottom end, of the outer wall of the circumference of the collecting tank, a fourth valve is arranged on the liquid discharge pipe, and the liquid discharge pipe is communicated with the inside of the collecting tank.

Preferably, the negative pressure mechanism comprises a barrel, a cavity is formed in the barrel, an air hole is formed in the top surface of the barrel, the air hole is communicated with the cavity, an exhaust pipe is coaxially arranged at the bottom end of the barrel, the exhaust pipe and the barrel are of an integrally formed structure, two ends of the exhaust pipe are respectively communicated with the cavity and the inside of the collection tank, an air cylinder is fixedly connected to the top surface of the barrel through a bolt, a piston rod of the air cylinder penetrates through the outer wall of the barrel and extends into the cavity, a piston is coaxially arranged at the end of the piston rod of the air cylinder, the piston is in sliding connection with the cavity, and a through hole is formed in the piston.

Preferably, check valves are respectively embedded in the air exhaust pipe and the through hole.

Preferably, the liquid inlet hopper is funnel-shaped in overall shape.

Preferably, the level of the extraction tube is higher than the level of the ultrafiltration membrane.

Preferably, the negative pressure mechanism is arranged obliquely, and an included angle between the negative pressure mechanism and the collecting tank is 45 degrees.

Compared with the prior art, the beneficial effects of the utility model are that: this special ultrafiltration device of exosome extraction separation is through the negative pressure mechanism that is equipped with, drive piston motion under the effect of cylinder piston pole promptly, and then form the atmospheric pressure difference between liquid storage pot and collection tank, thereby make the exosome extract the separation through the milipore filter under the effect of atmospheric pressure difference, this design has changed traditional milipore filter and has extracted the mode of exosome through long-time low-speed centrifugation, on the one hand, the time of extracting the separation has been reduced, on the other hand, the efficiency of extracting the separation has been improved, the problem that there is the drawback in the mode of traditional milipore filter extraction exosome has been solved.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic view of the structure of the liquid storage tank of the present invention;

FIG. 3 is a schematic structural view of an ultrafiltration module according to the present invention;

fig. 4 is a partial structural sectional view of the present invention;

FIG. 5 is a schematic structural view of a collection tank of the present invention;

fig. 6 is a structural sectional view of the middle negative pressure mechanism of the present invention.

In the figure: 1. a support; 11. a mounting cavity; 2. a liquid storage tank; 21. a liquid inlet pipe; 22. a first valve; 3. an ultrafiltration module; 31. a liquid inlet hopper; 32. a connecting pipe; 321. ultrafiltration membranes; 33. an extraction tube; 34. a second valve; 4. a collection tank; 41. a collection pipe; 42. a third valve; 43. a liquid discharge pipe; 44. a fourth valve; 5. a negative pressure mechanism; 51. a barrel; 511. air holes; 512. a cavity; 52. an air exhaust pipe; 53. a cylinder; 54. a piston; 541. and a through hole.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.

Furthermore, the terms "first", "second", "third" and "fourth" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, features defined as "first", "second", "third" and "fourth" may explicitly or implicitly include one or more of the features.

Example 1

An ultrafiltration device special for exosome extraction and separation is shown in fig. 1 to 5 and comprises a bracket 1, wherein a mounting cavity 11 is arranged at the front side of the bracket 1. The installation cavity 11 is internally provided with a hollow liquid storage tank 2, an ultrafiltration component 3 and a hollow collection tank 4 from top to bottom in sequence. The liquid storage tank 2 comprises a liquid inlet pipe 21, the liquid inlet pipe 21 is tightly welded with the oil storage tank 2, the liquid inlet pipe 21 is communicated with the liquid storage tank 2, the liquid inlet pipe 21 penetrates through the top surface of the support 1 and protrudes above the support, and a first valve 22 is arranged on the liquid inlet pipe 21. Ultrafiltration module 3 is including advancing liquid hopper 31, and it is linked together with liquid storage pot 2 to advance liquid hopper 31, the below of entering liquid hopper 31 is equipped with connecting pipe 32, it is linked together with connecting pipe 32 to advance liquid hopper 31, it is the integrated into one piece structure with connecting pipe 32 to advance liquid hopper 31, and the top of entering liquid hopper 31 and the top fixed connection of connecting pipe 32's bottom respectively through flange and collecting pipe 41, the inner wall of connecting pipe 32 is close to bottom department and inlays and is equipped with ultrafiltration membrane 321, be equipped with extraction tube 33 on the connecting pipe 32, extraction tube 33 and connecting pipe 32 closely weld, and extraction tube 33 and connecting pipe 32 are linked together, be equipped with second valve 34 on the extraction. The collection tank 4 includes collecting pipe 41, and collecting pipe 41's both ends are linked together with the inside of connecting pipe 32 and collection tank 4 respectively, and collecting pipe 41's both ends pass through the flange respectively with connecting pipe 32 and collection tank 4 fixed connection, be equipped with third valve 42 on the collecting pipe 41, the circumference outer wall of collection tank 4 is close to bottom department and is equipped with fluid-discharge tube 43, and fluid-discharge tube 43 and collection tank 4 closely weld, be equipped with fourth valve 44 on the fluid-discharge tube 43, and fluid-discharge tube 43 is linked together with the inside of collection tank 4.

In this embodiment, the whole shape of feed liquor fill 31 is hopper-shaped, and when the exosome entered into to connecting pipe 32 through the feed liquor fill 31 of hopper-shaped, under the effect that negative pressure and pipe diameter become little, the high-speed ultrafiltration membrane 321 that passes through of exosome had improved the efficiency of mentioning the separation.

Further, the level of the extraction tube 33 is higher than that of the ultrafiltration membrane 321, which facilitates the filtered exosome to be discharged to the outside for collection through the extraction tube 33.

Referring to fig. 6, the collecting tank 4 is provided with a negative pressure mechanism 5. Specifically, the negative pressure mechanism 5 includes a cylinder 51, a cavity 512 is provided inside the cylinder 51, an air hole 511 is provided on the top surface of the cylinder 51, the air hole 511 is communicated with the cavity 512, an air exhaust pipe 52 is coaxially provided at the bottom end of the cylinder 51, the air exhaust pipe 52 is tightly welded to the circumferential outer wall of the collection tank 4, the air exhaust pipe 52 and the cylinder 51 are of an integrally formed structure, two ends of the air exhaust pipe 52 are respectively communicated with the cavity 512 and the inside of the collection tank 4, a cylinder 53 is fixedly connected to the top surface of the cylinder 51 through a bolt, a piston rod of the cylinder 53 penetrates through the outer wall of the cylinder 51 and extends into the cavity 512, a piston 54 is coaxially provided at the end of the piston rod of the cylinder 53, the piston 54 is embedded at the end of the piston rod of the cylinder 53, the.

In this embodiment, check valves are respectively embedded in the air exhaust tube 52 and the through hole 541, the check valve in the air exhaust tube 52 facilitates the air in the collecting tank 4 to enter the cavity 512, so as to form a negative pressure in the collecting tank 4, and the check valve in the through hole 541 facilitates the air in the cavity 512 to be exhausted to the outside.

Further, negative pressure mechanism 5 is the slope setting, and negative pressure mechanism 5 and the contained angle between collection tank 4 are 45, and the negative pressure mechanism 5 of slope has prevented that impurity etc. after the filtration from getting into in negative pressure mechanism 5.

It should be noted that the cylinder 53 in this embodiment may be a thin cylinder of model SDA32 x 5 manufactured by shannais technologies group limited, and its associated pneumatic system, pipeline and solenoid valve may also be provided by the manufacturer.

Before the special ultrafiltration device for exosome extraction and separation in the embodiment is used, a user firstly closes the second valve 34, the third valve 42 and the fourth valve 44, then the user conveys exosomes into the oil storage tank 2 through the liquid inlet pipe 21, exosomes synchronously enter the liquid inlet hopper 31 and the connecting pipe 32, and at the moment, the user closes the first valve 22;

when the special ultrafiltration device for exosome extraction and separation in the embodiment is used, a user operates the air cylinder 53 to start working, the piston rod of the air cylinder 53 extends and drives the piston 54 to slide in the cavity 512, at the moment, air in the cavity 512 is exhausted to the outside through the through hole 541, when the piston rod of the air cylinder 53 contracts and drives the piston 54 to slide in the cavity 512, air in the collection tank 4 enters the cavity 512 at the moment, the above steps are repeated, the pressure in the collection tank 4 is gradually reduced, then the user opens the third valve 42, a pressure difference exists between the liquid storage tank 2 and the collection tank 4, under the action of the pressure difference, exosome passes through the ultrafiltration membrane 321 at a high speed, impurities and other ultrafiltration membranes 321 are filtered by the ultrafiltration membrane and fall into the collection tank 4 below, when the filtration is finished, the user opens the second valve 34, the extracted exosome flows out through the extraction pipe 33, at the same time, the user opens the fourth valve 44, and the impurities and the like are discharged to the outside through the drain pipe 43.

The foregoing shows and describes the general principles, essential features, and advantages of the invention. It should be understood by those skilled in the art that the present invention is not limited by the above embodiments, and the description in the above embodiments and the description is only preferred examples of the present invention, and is not intended to limit the present invention, and that the present invention can have various changes and modifications without departing from the spirit and scope of the present invention, and these changes and modifications all fall into the scope of the claimed invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. The utility model provides a special ultrafiltration device of exosome extraction separation, includes support (1), its characterized in that: the front side of the bracket (1) is provided with an installation cavity (11); a hollow liquid storage tank (2), an ultrafiltration component (3) and a hollow collection tank (4) are sequentially arranged in the mounting cavity (11) from top to bottom, and a negative pressure mechanism (5) is arranged on the collection tank (4); the liquid storage tank (2) comprises a liquid inlet pipe (21), the liquid inlet pipe (21) is communicated with the liquid storage tank (2), the liquid inlet pipe (21) penetrates through the top surface of the support (1) and protrudes above the support, and a first valve (22) is arranged on the liquid inlet pipe (21); the ultrafiltration assembly (3) comprises a liquid inlet hopper (31), the liquid inlet hopper (31) is communicated with the liquid storage tank (2), a connecting pipe (32) is arranged below the liquid inlet hopper (31), the liquid inlet hopper (31) is communicated with the connecting pipe (32), the liquid inlet hopper (31) and the connecting pipe (32) are of an integrally formed structure, an ultrafiltration membrane (321) is embedded in the position, close to the bottom end, of the inner wall of the connecting pipe (32), an extraction pipe (33) is arranged on the connecting pipe (32), the extraction pipe (33) is communicated with the connecting pipe (32), and a second valve (34) is arranged on the extraction pipe (33); the collecting tank (4) comprises a collecting pipe (41), the two ends of the collecting pipe (41) are respectively communicated with the connecting pipe (32) and the inside of the collecting tank (4), a third valve (42) is arranged on the collecting pipe (41), a liquid discharge pipe (43) is arranged on the position, close to the bottom end, of the outer wall of the circumference of the collecting tank (4), a fourth valve (44) is arranged on the liquid discharge pipe (43), and the liquid discharge pipe (43) is communicated with the inside of the collecting tank (4).

2. The special ultrafiltration device for exosome extraction and separation according to claim 1, characterized in that: the negative pressure mechanism (5) comprises a cylinder body (51), a cavity (512) is formed in the cylinder body (51), an air hole (511) is formed in the top surface of the cylinder body (51), the air hole (511) is communicated with the cavity (512), an air suction pipe (52) is coaxially arranged at the bottom end of the cylinder body (51), the air suction pipe (52) and the cylinder body (51) are of an integrally formed structure, two ends of the air suction pipe (52) are respectively communicated with the cavity (512) and the interior of the collection tank (4), the top surface of the cylinder body (51) is fixedly connected with an air cylinder (53) through bolts, a piston rod of the air cylinder (53) penetrates through the outer wall of the cylinder body (51) and extends into the cavity (512), a piston (54) is coaxially arranged at the end part of a piston rod of the air cylinder (53), and the piston (54) is in sliding connection with the cavity (512), the piston (54) is provided with a through hole (541).

3. The special ultrafiltration device for exosome extraction and separation according to claim 2, characterized in that: check valves are respectively embedded in the air extraction pipe (52) and the through hole (541).

4. The special ultrafiltration device for exosome extraction and separation according to claim 1, characterized in that: the liquid inlet hopper (31) is funnel-shaped in overall shape.

5. The special ultrafiltration device for exosome extraction and separation according to claim 1, characterized in that: the level of the extraction tube (33) is higher than the level of the ultrafiltration membrane (321).

6. The special ultrafiltration device for exosome extraction and separation according to claim 2, characterized in that: the negative pressure mechanism (5) is obliquely arranged, and an included angle between the negative pressure mechanism (5) and the collecting tank (4) is 45 degrees.