CN211306422U

CN211306422U - Device for accurately cutting coronal rat brain tissue

Info

Publication number: CN211306422U
Application number: CN201921782615.9U
Authority: CN
Inventors: 冯郸; 马洁; 楼敏铭
Original assignee: Tianjin Kangting Biological Engineering Group Co Ltd
Current assignee: Tianjin Kangting Biological Engineering Group Co Ltd
Priority date: 2019-10-23
Filing date: 2019-10-23
Publication date: 2020-08-21
Anticipated expiration: 2029-10-23

Abstract

The utility model relates to a device for accurately cutting the coronal section mouse brain tissue, which comprises a base, a left clamping part, a right clamping part, a mouse brain coronal section knife rest and a cutting blade, the front side and the rear side of the upper surface of the base are correspondingly and vertically provided with a mouse brain coronal section knife rest, the coronal section knife rest is correspondingly provided with a plurality of knife grooves corresponding to the inner side wall, the top end of the coronal section knife rest is provided with a scale lath, the scale lathes corresponding to the positions of the cutter grooves are uniformly and correspondingly provided with scales which are sequentially increased from right to left, a plurality of cutting blades are hung on the outer side wall of the crown-shaped section knife rest through hooks, the cutting blades are inserted into the knife grooves for cutting, the left baffle and the right baffle are symmetrically arranged on the left side and the right side of the upper surface of the base, and a left clamping part and a right clamping part are respectively arranged on the left baffle and the right baffle and used for clamping and stabilizing rat brain tissues. The utility model discloses it is stable, accurate to cut into slices to place the centre gripping.

Description

Device for accurately cutting coronal rat brain tissue

Technical Field

The utility model belongs to the technical field of it is biological, relate to brain tissue cutting technique, especially a device that is used for accurate coronal face mouse brain tissue of cutting.

Background

Brain tissue is the key part of many research works and experimental teaching such as nerves and spirit, generally rat and mouse brain tissue are selected as research objects, researchers want to observe the brain tissue, need to cut the brain tissue into a plurality of sections according to a rat brain map, take out the needed parts, dehydrate, soak wax, embed and slice, and finally observe the brain tissue through immunohistochemistry. However, the brain tissue parts are dislocated complicatedly, and how to find the parts needed by the brain tissue parts for tissue observation.

At present, scales are generally drawn on coordinate paper according to a brain atlas, and then, a brain is placed on the coordinate paper and cut by using an operating knife. However, the brain tissues are generally fixed in paraformaldehyde and are taken out and placed on the coordinate paper, and the paper is wet, so that the brain tissues can only be used once, the coordinate paper needs to be replaced for each brain tissue, the experiment is complicated, and the experiment time is wasted because coordinates need to be drawn for each brain tissue. Because the brain tissue can not be fixed when the device is placed on the coordinate paper, all brains sometimes move, and the brain tissue parts required by experimenters can not be accurately cut. And according to the scale of the coordinate paper, the brain tissue can not be accurately cut out because the approximate distance is identified by naked eyes. At present, a scalpel is used for cutting brain tissue, but the scalpel is narrow and wide, so that the scalpel cannot cut the section of the brain tissue to be flat, the error of the part is caused, and the whole tissue cannot be cut quickly when paraffin is sliced.

The following patent documents relevant to the present application are found by search, and specific disclosures are as follows:

1. a device and a method (CN106289856B) for manufacturing a brain tissue slice with a large section and a large thickness are provided, wherein the base is provided with a groove which is used for preventing the interference between a cutter and the base when the cutter cuts brain tissue; the top of the base is detachably connected with the positioning plate and the stop block respectively, and the height of the stop block is higher than that of the positioning plate; the baffle block is a cuboid, and the left side face of the baffle block is used as a reference surface for cutting the brain tissue into a section; the top surface and the bottom surface of the positioning plate are square, the side surface of the positioning plate is vertical to the top surface and the bottom surface, and the length of the positioning plate is the same as that of the groove; the positioning plate is provided with 5 guide grooves for guiding the cutting knife; any one guide groove is at least parallel to one side surface of the positioning plate; the guide grooves comprise at least 1 cutting block guide groove and 4 cutting block guide grooves; the 4 slicing guide grooves are respectively parallel to 4 side surfaces of the positioning plate, and the slicing guide grooves corresponding to the adjacent side surfaces are mutually vertical; the distance between the right side surface of the slice guide groove and the adjacent side surface of the positioning plate is the thickness of the section of the brain tissue; the distances from any one slice guide groove to the corresponding adjacent side of the positioning plate are different; when brain tissue slices with different thicknesses are cut, the orientation of a positioning plate is adjusted to enable a guide groove with a proper distance value to be parallel to the left side face of the stop block, and then a cutter is used for cutting; the positioning plate is also provided with 4 countersunk through holes with central symmetry, so that the left and right positions of the positioning plate on the base can be conveniently adjusted to enable the positioning plate to be tightly attached to the left side surface of the stop block.

2. The rat brain tissue positioning and repairing device (CN207336106U) comprises a substrate, wherein a groove which is similar to the shape of a rat brain tissue is formed in the top surface of the substrate, a cutter groove, a cerebellar bridge cutter groove, two midbrain cutter grooves and two thalamus cutter grooves which penetrate through the groove and are arranged in parallel along the length extending direction of the groove are vertically and longitudinally cut in the top surface of the substrate, and the cerebellar bridge cutter grooves are close to the cutter grooves.

3. A device (CN202547971U) for slicing and layering of mouse brain tissues comprises a slicing module, wherein a plurality of cutter grooves with equal intervals are formed in the slicing module, and when slicing is carried out, fresh mouse brain tissues are placed on the slicing module; the cutter is connected with the upper end of a vertical arm, and the lower end of the vertical arm is connected with a mechanism capable of moving and positioning along the length direction of the front surface of the operating platform.

Through the contrast of technical characteristics, above-mentioned patent literature of disclosing with the utility model discloses a technical structure is inequality, can not influence the creativity and the novelty of the utility model application.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to overcome prior art's weak point, provide one kind and place stable, the accurate device that is used for accurate cutting coronal face mouse brain tissue of centre gripping of section.

The utility model provides a its technical problem take following technical scheme to realize:

a device for accurately cutting coronal rat brain tissue comprises a base, a left clamping part, a right clamping part, a rat brain coronal section knife rest and cutting blades, wherein the rat brain coronal section knife rest is vertically installed on the front side and the rear side of the upper surface of the base correspondingly, a plurality of knife grooves are correspondingly formed in the coronal section knife rest corresponding to the inner side wall, scale slats protruding out of the outer side wall of the rat brain coronal section knife rest are formed at the top end of the coronal section knife rest, scales are correspondingly and uniformly arranged on the scale slats corresponding to the positions of the knife grooves and are sequentially increased from right to left, a plurality of cutting blades are hung on the outer side wall of the coronal section knife rest through hooks, the cutting blades are inserted into the knife grooves for cutting, a left baffle and a right baffle are further symmetrically installed on the left side and the right side of the upper surface of the base, and a left clamping part and a right clamping part are respectively arranged on the left baffle and, used for clamping and stabilizing the brain tissue of the rat.

And, right clamping part includes right track, connecting rod and right splint, right baffle lower part is provided with a right track that can adjust from top to bottom, perpendicular slidable mounting has the connecting rod in the track of the right side, the connecting rod has the round hole with right track connection mid portion, and the round hole is furnished with the bolt, a right splint is equipped with admittedly to the connecting rod tip, the plug has been laid to right splint internal surface, the leftmost end of right splint with 0 scale of coronal section knife rest top scale plate corresponds.

And, left clamping part includes left track, flexible arm and left splint, left baffle lower part is provided with a left track that can adjust from top to bottom, vertical slidable mounting has flexible arm in the track of a left side, flexible arm and left track connection mid portion have the round hole, and the round hole is furnished with the bolt, a left splint is equipped with admittedly to flexible arm one end, the plug has been laid to the splint internal surface, it has the round hole to go back equipartition interval system on the flexible arm, the round hole is furnished with the screw.

And, a plurality of waste liquid recovery holes are evenly distributed on the upper surface of the base at intervals, and a waste liquid outlet is also arranged on the side wall of the base.

Furthermore, the coronal section holder is a square solid plastic plate.

And, cutting blade includes cutting edge, cutter body and handle of a knife, the cutter body is a rectangle cutter body, the both sides and the sword groove looks adaptation of cutter body, the cutting edge is made to cutter body one end an organic whole, handle formula handle of a knife is made to the cutter body other end an organic whole, it has anti-skidding wearing layer to paste on the handle of a knife.

The utility model has the advantages that:

the utility model is provided with the adjustable left and right clamping parts, so that the placing and clamping are stable, and the damage to brain tissues is small; the knife grooves of the coronal section knife rest are arranged, so that the brain tissue can be cut accurately, quickly and smoothly, and brain tissue parts required by experiments can be cut accurately; this device is equipped with the scale lath, is of value to and need not draw coordinate paper once, and the scale can be used repeatedly to can wash, practice thrift the test time, improve experimental efficiency.

Drawings

FIG. 1 is a schematic structural view of the present invention;

fig. 2 is a perspective view of the present invention.

Detailed Description

The present invention will be described in further detail with reference to the following embodiments, which are illustrative, not restrictive, and the scope of the invention should not be limited thereto.

The device for accurately cutting the coronary mouse brain tissue comprises a base 15, a left clamping part, a right clamping part, a mouse brain coronary face cutting knife rest 13 and a cutting blade 10, wherein the base is of a cuboid structure, the mouse brain coronary face cutting knife rest is correspondingly and vertically arranged on the front side and the rear side of the upper surface of the base, the coronary face cutting knife rest is a square solid plastic plate with the length of 5 cm and the width of 4 cm, the corresponding inner side surfaces of the coronary face cutting knife rest are respectively provided with a thirty-eight cutter groove 11 with the size of 1 mm, the two outer side surfaces at the top end of the coronary face cutting knife rest are respectively provided with a scale lath 12 protruding the outer side surface 1 cm of the mouse brain coronary face cutting knife rest, the scale laths corresponding to the positions are uniformly and correspondingly provided with scales which are sequentially increased from right to left, the outer side surface of the coronary face cutting knife rest is further provided with a hook 17, and the hook can be, the cutting blade comprises a cutting edge, a cutter body and a cutter handle, the cutter body is a rectangular cutter body, the two sides of the cutter body are matched with the cutter groove, the cutting edge is integrally formed at one end of the cutter body, the handle type cutter handle is integrally formed at the other end of the cutter body, and an anti-skidding and anti-abrasion layer is pasted on the cutter handle.

The upper surface of the base between the coronal section knife rests is symmetrically provided with a left baffle 1 and a right baffle 14, the left baffle and the right baffle are respectively provided with a left clamping part and a right clamping part, the right clamping part comprises a right rail 22, a connecting rod 21 and a right clamping plate 20, the lower part of the right baffle is provided with a right rail which can be adjusted up and down, the connecting rod is vertically and slidably arranged in the right rail, the connecting rod is connected with the right rail, the middle part of the connecting rod is provided with a round hole, the round hole is provided with a bolt, the distance between the connecting rod and the base can be adjusted, the end part of the connecting rod is fixedly provided with the right clamping plate, the inner surface of the right clamping plate is paved with a rubber plug 19, so that brain tissues are prevented from being damaged by the clamping plate during clamping, and the rubber plug at the leftmost end of the right clamping;

the left clamping part comprises a left rail 4, a telescopic arm 5 and a left clamp plate 7, the lower part of the left baffle plate is provided with the left rail, the telescopic arm is vertically and slidably mounted in the left rail, the middle part of the telescopic arm, which is connected with the left rail, is provided with a round hole 2, the round hole is provided with a bolt 3, the distance between the telescopic arm and the base can be adjusted, one end of the telescopic arm is fixedly provided with the left clamp plate, the inner surface of the clamp plate is paved with a rubber plug 18 to avoid the damage of the clamp plate to brain tissues during clamping, the telescopic arm is also provided with round holes at intervals, the round holes are provided with screws 6, the left rail, the right rail and the telescopic arm are adjusted according to the mouse brain specifications of rats and mice, after the fixed position is determined, the clamping height of the telescopic arm and the connecting rod is fixed by the screws, and the distance between the left clamp plate, thereby realizing stable clamping of the mouse brain.

The utility model discloses an in the concrete implementation, in order to make the waste liquid when cutting the brain in time discharge, base upper surface equipartition interval system has a plurality of waste liquid recovery holes 8, base one side still is provided with a waste liquid discharge port 16.

The utility model discloses when using, at first place rat brain 9 on the base, according to the height of the flexible arm and the connecting rod of the interior track of altitude mixture control left and right sides of rat brain prepare the height of base, then contact the bregma of rat brain with right splint, then adjust flexible arm, stabilize the centre gripping with the rat brain, then carry out the accurate cutting of cutting blade realization in corresponding the sword inslot interpolation according to the thickness of required brain tissue.

Although the embodiments of the present invention and the accompanying drawings are disclosed for illustrative purposes, those skilled in the art will appreciate that: various substitutions, changes and modifications are possible without departing from the spirit and scope of the present invention and the appended claims, and therefore, the scope of the present invention is not limited to the disclosure of the embodiments and the accompanying drawings.

Claims

1. A device for accurately cutting the brain tissue of a coronal mouse is characterized in that: including base, left clamping part, right clamping part, mouse brain coronal section knife rest and cutting blade, the mouse brain coronal section knife rest is installed perpendicularly to both sides correspondence around the base upper surface, coronal section knife rest corresponds a pair of system of inside wall homogeneous and has a plurality of sword grooves, coronal section knife rest top all has the scale lath of protrusion mouse brain coronal section knife rest lateral wall, and homogeneous corresponds on the scale lath that corresponds the sword groove position and is provided with the scale, the scale scales increase progressively from right side to left side in proper order, a plurality of cutting blades have been hung through the couple on the lateral wall of coronal section knife rest, cutting blade inserts the sword groove and cuts, left baffle and right baffle are still installed to base upper surface left and right both sides symmetry, be provided with left clamping part and right clamping part on left baffle and the right baffle respectively for the stable mouse brain tissue of centre gripping.

2. The device for precision coronal murine brain tissue according to claim 1, characterized by: the right clamping part comprises a right rail, a connecting rod and a right clamping plate, the lower part of the right baffle is provided with the right rail which can be adjusted up and down, the connecting rod is vertically and slidably mounted in the right rail, the connecting rod is connected with the middle part of the right rail through a round hole, the round hole is provided with a bolt, the end part of the connecting rod is fixedly provided with the right clamping plate, the rubber plug is paved on the inner surface of the right clamping plate, and the leftmost end of the right clamping plate corresponds to 0 scale of the top end scale plate of the coronal section knife rest.

3. The device for precision coronal murine brain tissue according to claim 1, characterized by: the utility model discloses a telescopic arm, including left baffle, telescopic arm, bolt, plug, the left clamping part includes left track, flexible arm and left splint, left baffle lower part is provided with a left track that can adjust from top to bottom, perpendicular slidable mounting has flexible arm in the track of a left side, flexible arm and left track connection mid portion have the round hole, and the round hole is furnished with the bolt, a left splint are equipped with admittedly to flexible arm one end, the plug has been laid to the splint internal surface, it has the round hole to go back equipartition interval system on the flexible arm, the round hole is.

4. The device for precision coronal murine brain tissue according to claim 1, characterized by: a plurality of waste liquid recovery holes are uniformly distributed on the upper surface of the base at intervals, and a waste liquid discharge port is further formed in the side wall of the base.

5. The device for precision coronal murine brain tissue according to claim 1, characterized by: the coronal section holder is a square solid plastic plate.

6. The device for precision coronal murine brain tissue according to claim 1, characterized by: the cutting blade comprises a cutting edge, a cutter body and a cutter handle, the cutter body is a rectangular cutter body, the two sides of the cutter body are matched with the cutter groove, the cutting edge is integrally formed at one end of the cutter body, the handle type cutter handle is integrally formed at the other end of the cutter body, and an anti-skidding and anti-abrasion layer is pasted on the cutter handle.