CN211199191U

CN211199191U - Magnetic adsorption device for micro centrifugal tube

Info

Publication number: CN211199191U
Application number: CN201921653844.0U
Authority: CN
Inventors: 杨冰; 杨博然; 闫亮亮; 李文佳
Original assignee: Tianjin Prius Biotechnology Co ltd
Current assignee: Tianjin Prius Biotechnology Co ltd
Priority date: 2019-09-30
Filing date: 2019-09-30
Publication date: 2020-08-07
Anticipated expiration: 2029-09-30

Abstract

The utility model relates to a miniature centrifuging tube magnetic force adsorption equipment sets up a plurality of trompils at the support body up end, can set up the magnetic force cover in every trompil, and this magnetic force cover outer fringe contacts with the trompil inner edge, can imbed miniature centrifuging tube in this magnetic force cover, and the lower extreme that the magnetic force cover is located the support body below is for the elasticity end of from top to bottom gradually leanin, imbeds the permanent magnet in elasticity end lower extreme. The utility model discloses in, because the permanent magnet pastes the position that leans on in miniature centrifuging tube bottom under the effect of elasticity end, the magnetic bead in miniature centrifuging tube this moment has certain distance by the bottom in adsorption column and these magnetic beads and the miniature centrifuging tube, makes pipetting gun or pipetting pump take waste buffer out as far as. Overall structure is simple, can make by the working of plastics, easily dismouting, be convenient for wash, and the magnetic force cover of different internal diameters can adapt to the miniature centrifuging tube of different specifications, low cost makes things convenient for a large amount of purchases moreover.

Description

Magnetic adsorption device for micro centrifugal tube

Technical Field

The utility model relates to a magnetic adsorption structure improvement for DNA separationTechnical FieldIn particular to a magnetic adsorption device of a micro centrifugal tube.

Background

The traditional DNA separation and purification process often uses centrifugation, the used equipment is a centrifuge, the centrifuge has high rotating speed, so the centrifuge tube has to have good quality, the cost is increased, the centrifugation process is time-consuming, and more times of centrifugation are time-consuming, along with the development of scientific technology, a magnetic bead method is developed for extracting nucleic acid by a magnetic bead method, the magnetic bead method uses nanotechnology to improve and modify the surface of superparamagnetic nanoparticles, the magnetic beads can be specifically identified and efficiently combined with nucleic acid molecules on a microscopic interface, DNA and RNA are separated, the publication number is CN209397190U, the patent name is a magnetic force rack for nucleic acid purification, the magnetic force rack discloses a magnetic force device which triggers a switch by inserting a reaction tube and electrifys an electromagnet and a corresponding indicator lamp, so as to generate a magnetic field for purification, the patent has the following problems that 1, a large number of experiments in a laboratory environment need frequent cleaning equipment, the patent includes electromagnets and contacts, springs and the like, which cause inconvenient cleaning, 2, the overall structure is too complex, the use is convenient, the use is very convenient, the operation of a small scale of the centrifuge tube is not easy to be purchased, the centrifuge tube is not easy to be used, the centrifuge tube is not suitable for a small scale, the centrifuge tube is 2, the centrifuge tube is not suitable for a small scale, the test is not suitable for the test, the test is not suitable for the test of a small scale, the test of the test tube, the test tube is not suitable for the test tube is not.

SUMMERY OF THE UTILITY MODEL

The utility model aims to overcome the not enough of prior art, provide the trompil on the support body and place the magnetic force cover of different internal diameters and adapt to the miniature centrifuging tube of different specifications and easily install, maintain, be convenient for wash low cost's a miniature centrifuging tube magnetic force adsorption equipment.

The utility model adopts the technical proposal that:

the utility model provides a miniature centrifuging tube magnetic force adsorption equipment which characterized in that: including the support body, set up a plurality of trompils at the support body up end, every trompil can set up the magnetic force cover, and this magnetic force cover outer fringe contacts with the trompil inner edge, can imbed miniature centrifuging tube in this magnetic force cover, and the lower extreme that the magnetic force cover is located the support body below is for from top to bottom gradually the elasticity end of leanin, embeds the permanent magnet in elasticity end lower extreme.

Furthermore, the miniature centrifugal tube is inserted into the magnetic sleeve and then is externally extruded with an elastic end, and the permanent magnet in the elastic end is pressed on the outer edge of the inclined section at the lower end of the miniature centrifugal tube under the action of elastic force.

And the permanent magnet is used for attracting the magnetic beads in the miniature centrifugal tube and enabling the magnetic beads to leave the bottom in the miniature centrifugal tube.

Furthermore, the outer edge of the magnetic sleeve and the inner edge of the opening are embedded with each other through the convex edges and the grooves, or the outer edge of the magnetic sleeve and the inner edge of the opening are embedded with each other through the convex blocks and the concave pits, or the magnetic sleeve is in interference fit with the opening.

Furthermore, the outer edges of the plurality of magnetic sleeves on the upper end surface of the frame body are integrally provided with a connecting plate, and the side edges of the connecting plate are provided with an integral holding end.

Furthermore, the lower end of the frame body is connected with the upper end surface of a base plate, and the bottom surface of the base plate is provided with an anti-slip pad.

Furthermore, the lower end of the frame body is provided with tenons, and each tenon is embedded into a mortise arranged in the alignment of the base plate.

Furthermore, the side face of the tenon and the mortise are embedded with each other through the convex edge and the groove, or the side face of the tenon and the mortise are embedded with each other through the convex block and the concave, or the tenon and the mortise are in interference fit.

The utility model has the advantages that:

the utility model discloses in, the support body trompil, the embedded magnetic force cover in the trompil, magnetic force cover have multiple specification, and its internal diameter is different to adapt to the embedding of the miniature centrifuging tube of different specifications, in the magnetic force cover can be horizontal, fore-and-aft embedding trompil, specifically use the quantity decision according to miniature centrifuging tube. And (3) placing magnetic beads into the micro centrifugal tube, adding buffer solution after reaction for cleaning for multiple times, and after each cleaning, pumping out waste buffer solution at the bottom of the micro centrifugal tube by using a liquid transfer gun or a liquid transfer pump and continuing to clean for the next time. Because the permanent magnet pastes the position that leans on at miniature centrifuging tube bottom under the effect of elasticity end, the magnetic bead in miniature centrifuging tube is adsorbed the post and these magnetic beads have certain distance with the bottom in the miniature centrifuging tube this moment, makes pipetting gun or pipetting pump can take out useless buffer solution as far as. Overall structure is simple, can make by the working of plastics, easily dismouting, be convenient for wash, and the magnetic force cover of different internal diameters can adapt to the miniature centrifuging tube of different specifications, low cost makes things convenient for a large amount of purchases moreover.

Drawings

Fig. 1 is a schematic structural diagram of the present invention;

FIG. 2 is a sectional view taken along line A-A of FIG. 1;

FIG. 3 is a schematic drawing of the second microcentrifuge tube from the left in FIG. 1 for withdrawing waste buffer;

FIG. 4 is an enlarged view of section I of FIG. 2 (with the microcentrifuge tube removed from the magnetic sleeve);

FIG. 5 is a schematic view of the magnetic sleeve with a trumpet-shaped lower end;

fig. 6 is an enlarged view of a portion II of fig. 2.

Detailed Description

The present invention is further illustrated by the following examples, which are intended to be illustrative, not limiting and are not intended to limit the scope of the invention.

The utility model provides a miniature centrifuging tube magnetic force adsorption equipment, as shown in fig. 1 ~ 6, the utility model discloses an innovation lies in: including support body 1, set up a plurality of trompils 24 at the support body up end, every trompil can set up magnetic sleeve 9, and this magnetic sleeve outer fringe contacts with the trompil inner edge, and this magnetic sleeve can imbed miniature centrifuging tube 4, 5, 7, and the lower extreme that the magnetic sleeve is located the support body below is for from top to bottom gradually leanin's elasticity end 14, imbeds permanent magnet 15 in the arch 10 of elasticity end lower extreme.

In this embodiment, the microcentrifuge tube has model 7 of 0.5ml, model 5 of 1.5ml and model 4 of 2.0ml, and three kinds of microcentrifuge tubes are supporting three kinds of magnetic sleeves respectively, and as can be known from fig. 2, the trompil of support body is fixed size, but the internal diameter 25 of magnetic sleeve is different. The larger the capacity of the micro centrifugal tube is, the larger the inner diameter of the magnetic sleeve corresponding to the micro centrifugal tube is, and the smaller the inner diameter of the magnetic sleeve is. The permanent magnet can be selected according to actual needs.

The magnetic sleeve can be made of plastic with certain elasticity, so that the elastic end can be a sleeve end expansion structure similar to that in an expansion pipe, for example, in fig. 4, the elastic end which is inclined inwards is composed of two symmetrical sheet-shaped plates, and a permanent magnet is embedded in the lower end of the sheet-shaped plate at the left side in fig. 4. When the micro centrifugal tube is inserted, the two sheet-shaped plates are pressed outwards, the sheet-shaped plate on the left side is pressed on the outer edge of the inclined section 12 at the lower end of the micro centrifugal tube under the action of elastic force, and the magnetic field of the permanent magnet can adsorb magnetic beads in the micro centrifugal tube as shown in fig. 3.

The magnetic sleeve may also be made of a harder rubber, in which case the flexible end has a trumpet-like structure 26 as shown in fig. 5, and the trumpet-like structure gradually narrows from top to bottom. When the micro centrifugal tube is inserted, the horn-shaped structure is integrally propped open, and the permanent magnet is attached to the outer edge of the inclined section at the lower end of the micro centrifugal tube.

Whichever elastic end is used, the permanent magnet attracts the magnetic beads in the microcentrifuge tube and moves the magnetic beads away from the bottom of the microcentrifuge tube as shown in FIG. 3.

The outer edge and the inner edge of the opening of the magnetic sleeve are embedded with each other through the convex edge 19 and the groove 20, or the outer edge and the inner edge of the opening of the magnetic sleeve are embedded with each other through the convex block and the concave, or the magnetic sleeve is in interference fit with the opening.

In order to improve the intensity of magnetic sleeve and the convenience of dismouting, a plurality of magnetic sleeves are located the outer fringe an organic whole of support body up end and are provided with connecting plate 2, for example: two magnetic sleeves are shown in fig. 1 as a group, three, four or more magnetic sleeves may be shown as a group, and a group of one magnetic sleeve is also possible. Regardless of the number of the magnetic sleeves, the side edges of one group of the connecting plates are provided with an integrated holding end 3 as shown in figure 1, and the holding end can be conveniently held by hands and is convenient for the installation and the disassembly of the magnetic sleeves.

The lower end 16 of the frame is connected to the upper end of a base plate 13, and the bottom of the base plate is provided with a non-slip mat 11. The lower end of the frame body is provided with a tenon 17, and each tenon is embedded into a mortise arranged on the base plate in an alignment way. The side face of the tenon and the mortise are embedded with each other through the convex edge and the groove, or the side face of the tenon and the mortise are embedded with each other through the convex block and the concave part, or the tenon and the mortise are in interference fit.

The utility model discloses a process is:

1. selecting a proper frame body, and then installing the frame body on a base plate, wherein as shown in figure 1, 10 holes are formed in the frame body, two magnetic sleeves are positioned on the same connecting plate and vertically embedded into the holes in the figure;

2. a magnetic sleeve is embedded in the opening, and the specification and the number of the magnetic sleeve are determined by the specification and the number of the micro centrifugal tubes used in the experiment;

3. adding the substance to be purified into a miniature centrifugal tube, and adding enzyme for treatment after cracking;

4. adding magnetic beads 21 for reaction;

5. after treatment, inserting the micro centrifugal tube into the magnetic sleeve, adding a buffer solution into the micro centrifugal tube for cleaning for multiple times, and buckling an upper cover 8 of the micro centrifugal tube during cleaning to shake the frame body so that the buffer solution can clean the inner wall of the whole micro centrifugal tube;

after each washing, the lower end 22 of the liquid transfer gun 18 is extended to the bottom in the miniature centrifugal tube as shown in figure 3, the waste buffer solution 23 is gradually pumped out, and then the buffer solution is added again for washing again;

6. and finally, taking out the magnetic beads for subsequent treatment.

7. And (3) disassembling the substrate, the frame body and the magnetic sleeve, soaking the substrate, the frame body and the magnetic sleeve into a clean buffer solution, adding enzyme for reaction, washing and drying the substrate for the next test.

Claims

1. The utility model provides a miniature centrifuging tube magnetic force adsorption equipment which characterized in that: including the support body, set up a plurality of trompils at the support body up end, every trompil can set up the magnetic force cover, and this magnetic force cover outer fringe contacts with the trompil inner edge, can imbed miniature centrifuging tube in this magnetic force cover, and the lower extreme that the magnetic force cover is located the support body below is for from top to bottom gradually the elasticity end of leanin, embeds the permanent magnet in elasticity end lower extreme.

2. The magnetic adsorption device of a micro centrifugal tube according to claim 1, wherein: the miniature centrifuging tube inserts outside extrusion elasticity end after the magnetic sleeve, and the permanent magnet in the elasticity end pushes down the slope section outer fringe at miniature centrifuging tube lower extreme under the effect of elasticity.

3. The magnetic adsorption device of a micro centrifugal tube according to claim 1, wherein: the permanent magnet is used for attracting the magnetic beads in the miniature centrifugal tube and enabling the magnetic beads to leave the bottom in the miniature centrifugal tube.

4. The microcentrifuge tube magnetic adsorption device of claim 1, 2 or 3, which is characterized in that: the outer edge of the magnetic sleeve and the inner edge of the opening are embedded with each other through the convex edges and the grooves, or the outer edge of the magnetic sleeve and the inner edge of the opening are embedded with each other through the convex blocks and the concave pits, or the magnetic sleeve is in interference fit with the opening.

5. The microcentrifuge tube magnetic force adsorption device of claim 4, which is characterized in that: the outer edges of the plurality of magnetic sleeves on the upper end face of the frame body are integrally provided with connecting plates, and the side edges of the connecting plates are provided with integrated holding ends.

6. The microcentrifuge tube magnetic force adsorption device of claim 5, characterized in that: the lower end of the frame body is connected with the upper end face of a base plate, and the bottom face of the base plate is provided with an anti-slip pad.

7. The magnetic adsorption device of a micro centrifugal tube according to claim 6, wherein: the lower end of the frame body is provided with tenons, and each tenon is embedded into a mortise arranged in the alignment of the base plate.

8. The microcentrifuge tube magnetic force adsorption device of claim 7, which is characterized in that: the side face of the tenon and the mortise are embedded with each other through the convex edge and the groove, or the side face of the tenon and the mortise are embedded with each other through the convex block and the concave part, or the tenon and the mortise are in interference fit.