CN211199189U - DNA purification is with sliding connection formula electrophoresis apparatus - Google Patents

Abstract

The utility model relates to a DNA purification is with sliding connection formula electrophoresis apparatus sets up a plurality of mutual isolation's electrophoresis cell in the electrophoresis box, and the plate electrode that every electrophoresis cell both sides set up realizes the electricity with the binding post of the cooling box lateral wall setting of homonymy respectively through gliding mode and connects, and this binding post is connected with the power electricity, and lock upper cover on the cooling box remains the clearance between electrophoresis box and the cooling box, this clearance respectively with the refrigerant import and the refrigerant export intercommunication that the cooling box surface set up. The utility model discloses in, can carry out the secondary electrophoresis to a plurality of gel cuttings simultaneously in every electrophoresis box, make the DNA molecular fragment on it remove in the buffer solution, then adsorb the recovery to the DNA molecular fragment in the buffer solution, the voltage of plate electrode circular telegram can appropriate improvement to improve the removal rate of DNA molecular fragment, and the introduction of refrigerant has avoided the gel because of the rising of buffer solution temperature and the problem of dissolving in the buffer solution, has guaranteed the purity that later stage DNA molecular fragment retrieved.

Description

DNA purification is with sliding connection formula electrophoresis apparatus

Technical Field

The utility model belongs to the improved technology of electrophoresis equipment structureFIELDIn particular to a sliding connection type electrophoresis device for DNA purification.

Background

In DNA separation experiments, electrophoresis equipment is often used, which uses the action of an electric field to move DNA molecular fragments to different positions on agarose gel, then cuts the gel into pieces, adsorbs the DNA molecular fragments by adsorption columns or magnetic beads, and then elutes the pieces to obtain the desired DNA molecular fragments. The above separation treatment has the following problems: 1. the traditional electrophoresis equipment has the plug on the bottom box, and the upper cover has the socket, after the upper cover is buckled on the bottom box, one end of the socket is embedded into the plug, and the other end of the socket is connected with the power supply through the cable, so that the buckling process of the upper cover is complex and inconvenient to operate; 2. since the gel is composed of agarose, which needs to be dissolved in a solvent by heating before adsorption, agarose is also adsorbed as an impurity of the DNA molecule fragments during the separation process, which causes the purity of the DNA molecule fragments to be lowered, and is not favorable for the subsequent test.

Disclosure of Invention

The utility model aims to overcome the not enough of prior art, provide and integrate a plurality of electrophoresis cell in an electrophoresis box to be provided with a DNA purification of the cooling box and the slip electric connection structure who is used for cooling off the electrophoresis box and use sliding connection formula electrophoresis apparatus.

The technical scheme that this practicality was taken is:

a sliding connection type electrophoresis device for DNA purification is characterized in that: including cooling box, electrophoresis box and upper cover, place at least one electrophoresis box in the cooling box, set up a plurality of mutual isolation's electrophoresis cell in the electrophoresis box, the plate electrode that every electrophoresis cell both sides set up realizes the electricity with the binding post of the cooling box lateral wall setting of homonymy respectively through gliding mode and connects, and this binding post is connected with the power electricity, and lock upper cover on the cooling box remains the clearance between electrophoresis box and the cooling box, this clearance respectively with the coolant import and the coolant export intercommunication that cooling box surface set up.

And moreover, a plurality of partition plates are arranged in the electrophoresis box, the electrophoresis box is internally divided into a plurality of electrophoresis tanks by the partition plates, the bottom plate of the electrophoresis box in each electrophoresis tank is provided with a bulge, the gel cutting area is arranged on each bulge, and the electrophoresis tanks on two sides of each bulge are electrode plate areas.

And moreover, the inner parts of the side walls of the two sides of the electrophoresis box are respectively provided with a bending plate, one side of the bending plate in the electrophoresis box is provided with an end part which is bent downwards and extends into the electrophoresis tank and forms an electrode plate, and the end part of the other side of the bending plate is electrically connected with the wiring terminal.

And a connecting point is arranged at the other side end part of the bending plate, the outer side surface of the connecting point is arc-shaped, and the arc-shaped protrudes to the outer side of the side wall of the electrophoresis box and is in sliding contact with a wiring terminal arranged in an alignment way with the cooling box.

Furthermore, the outer edge of the wiring terminal is provided with external threads and is meshed with the side wall surface aligned with the arc of the cooling box, the end part of the inner side of the wiring terminal is in sliding contact with the arc, the end part of the outer side of the wiring terminal is provided with a wiring hole and a fixing bolt, the wiring hole is used for inserting the end part of a cable connected with a power supply, and the fixing bolt is used for fixing the end part of the cable in the end part of the outer side of the wiring terminal.

And the upper end of the outer edge of the electrophoresis box is provided with at least one sealing ring from top to bottom, the outer edge of the electrophoresis box below the sealing ring is provided with a recess, the gap comprises a recess, the bottom surface of the electrophoresis box and the inner bottom surface of the cooling box, the lower end of the surface of one side of the cooling box is provided with a refrigerant inlet, and the upper end of the surface of the other side of the cooling box is provided with a refrigerant outlet.

Furthermore, the gap also includes a space inside the protrusion.

This practical advantage is with positive effect:

in the utility model, set up at least one electrophoresis box in the cooling box, separate into a plurality of electrophoresis cell through the baffle in the electrophoresis box, be provided with gel stripping and slicing area in every electrophoresis cell, the electrophoresis cell of gel stripping and slicing both sides is the plate electrode region, and the plate electrode in every electrode plate region is connected through board, arc and the binding post electricity of bending. When the electrophoresis device is used, air or liquid refrigerant is introduced into a gap between the cooling box and the electrophoresis boxes, buffer solution in the electrophoresis tank is cooled, then gel blocks are put into the electrophoresis boxes, the electrode plates are electrified, secondary electrophoresis can be simultaneously carried out on a plurality of gel blocks in each electrophoresis box, DNA molecular fragments on the gel blocks move into the buffer solution, then the DNA molecular fragments in the buffer solution are adsorbed and recovered, the electrified voltage of the electrode plates can be properly improved, so that the moving speed of the DNA molecular fragments is improved, the problem that the gel is dissolved in the buffer solution due to the temperature rise of the buffer solution is avoided due to the introduction of the refrigerant, and the recovery purity of the DNA molecular fragments in the later period is ensured.

Drawings

FIG. 1 is a schematic diagram of the construction of the present invention (two electrophoresis cassettes with the upper cover removed);

FIG. 2 is a schematic diagram of the structure of an electrophoresis cassette;

FIG. 3 is a left side view of FIG. 2;

FIG. 4 is a rear view of FIG. 3;

FIG. 5 is an enlarged cross-sectional view of FIG. 3;

fig. 6 is a positional relationship diagram of the separator and the electrode plate of fig. 5.

Detailed Description

The present invention is further illustrated by the following examples, which are intended to be illustrative, not limiting and are not intended to limit the scope of the invention.

A sliding connection type electrophoresis device for DNA purification is disclosed, as shown in figures 1-6, the innovation of the invention is as follows: including cooling box 1, electrophoresis box 11 and upper cover 17, place at least one electrophoresis box in the cooling box, set up a plurality of mutual isolation's electrophoresis cell 10 in the electrophoresis box, the plate electrode 21 that every electrophoresis cell both sides set up realizes the electricity with the binding post 6 of the cooling box lateral wall setting of homonymy respectively through gliding mode and is connected, this binding post is connected with the power electricity, the lock upper cover on the cooling box, remain clearance 28 between electrophoresis box and the cooling box, this clearance respectively with the coolant import 16 and the coolant export intercommunication 13 of cooling box surface setting.

In this embodiment, set up a plurality of baffles 4 in the electrophoresis box, this a plurality of baffles separate the electrophoresis box for a plurality of electrophoresis cell, and electrophoresis box bottom plate in every electrophoresis cell all is provided with arch 22, and two border departments that this arch is close to the electrophoresis cell both sides set up a riser 5 respectively, are gel stripping and slicing region 8 between two risers on every arch, and the electrophoresis cell of protruding both sides is electrode plate region 7, 9, places gel stripping and slicing 23 in gel stripping and slicing region.

The inside board 25 that bends that all sets up of electrophoresis box both sides lateral wall, the one side that the board lies in the electrophoresis box of bending is provided with the tip 21 that extends to the electrophoresis tank in the same downwarping with electrophoresis cell quantity and forms the plate electrode, and the opposite side tip and the binding post electricity of the board of bending are connected. The upper end of the inner surface of the electrophoresis box is provided with a transverse insulating strip 3, the bending plate is integrally connected with a transverse conductive plate in the insulating strip, and the bottom surface of the conductive plate is integrally provided with the plurality of electrode plates. The preferred scheme is as follows: the other side end of the bending plate is provided with a connecting contact point 20, the outer side surface of the connecting contact point is provided with an arc shape, and the arc shape protrudes to the outer side of the side wall of the electrophoresis box and is in sliding contact with a connecting terminal arranged in a contraposition with the cooling box.

The outer edge of the convex ring 14 at the lower end of the wiring terminal is provided with external threads and is meshed and connected with the surface of the side wall of the cooling box aligned with the connecting contact point, the end part 26 at the inner side of the wiring terminal is contacted with the arc-shaped surface of the connecting contact point, the end part at the outer side of the wiring terminal is provided with a wiring hole 12 and a fixing bolt 15 as shown in figure 3, the wiring hole is used for inserting the end part of a cable connected with a power supply, and the fixing bolt is used for fixing the end part of the cable in the.

At least one sealing ring 27 is arranged at the upper end of the outer edge of the electrophoresis box from top to bottom, two sealing rings are arranged up and down in the figure, the two sealing rings can be sleeved in a sealing ring groove arranged at the outer edge of the electrophoresis box, not only can the gap below be sealed, but also the electrophoresis box is stably embedded into the cooling box through friction.

The outer edge of the electrophoresis cassette below the sealing ring is provided with a recess 31, and the gap comprises the recess, a gap 29 between the bottom surface of the electrophoresis cassette and the inner bottom surface of the cooling cassette, and a space 30 inside the protrusion. The lower end of one side surface of the cooling box is provided with a refrigerant inlet, the upper end of the other side surface of the cooling box is provided with a refrigerant outlet, and after the refrigerant enters the gap, the outer edge and the bottom surface of the electrophoresis box are soaked in the refrigerant.

The cooling box, the electrophoresis box and the upper cover are made of transparent insulating materials, the bending plate, the conductive plate and the electrode plate are made of platinum materials integrally, and the wiring terminal is made of copper materials.

As shown in fig. 2, the electrophoresis chamber is divided into four electrophoresis tanks, each of which has a different capacity, and is 1 ml, 2 ml, 5 ml and 10 ml from the rightmost side to the leftmost side. The refrigerant can be gas or liquid, and the gas can be cold air cooled by a compressor, or ice-salt mixture (the particles of ice are small).

The upper end of the outer edge of the electrophoresis box is provided with a convex rib or a spaced convex block 2, so that the electrophoresis box can be stably placed on the upper end surface 19 of the cooling box. The upper cover is directly buckled on the upper end surface 18 of the cooling box at the outer side of the lug as shown in figure 5, and the electrophoresis box is not provided with a plug and a socket in the prior art, so that the electrophoresis box is convenient to install and detach.

This utility is shown in figure 2:

in this embodiment, only one electrophoresis cell is used, and the inside of the electrophoresis cell is divided into four electrophoresis cells with different volumes. And connecting all the connecting terminals with a power supply through cables. The refrigerant is ice salt mixture. The third 5 ml cell from the right of FIG. 2 was selected.

1. Agarose gel sections were placed into the gel section area of the electrophoresis cell.

2. The TAE buffer 24 was poured in, the ice-salt mixture was pumped in through a water pump, and the electrode plates were powered on when the temperature of the buffer was measured to be 5 ℃.

3. After a period of time, the power is turned off, the buffer is removed with a pipette, and the DNA fragments are further separated by adsorption.

In the invention, at least one electrophoresis box is arranged in a cooling box, the electrophoresis box is divided into a plurality of electrophoresis tanks by partition plates, a gel cutting area is arranged in each electrophoresis tank, the electrophoresis tanks on two sides of the gel cutting area are electrode plate areas, and an electrode plate in each electrode plate area is electrically connected with a connecting terminal through a bending plate and an arc. When the electrophoresis device is used, air or liquid refrigerant is introduced into a gap between the cooling box and the electrophoresis boxes, buffer solution in the electrophoresis tank is cooled, then gel blocks are put into the electrophoresis boxes, the electrode plates are electrified, secondary electrophoresis can be simultaneously carried out on a plurality of gel blocks in each electrophoresis box, DNA molecular fragments on the gel blocks move into the buffer solution, then the DNA molecular fragments in the buffer solution are adsorbed and recovered, the electrified voltage of the electrode plates can be properly improved, so that the moving speed of the DNA molecular fragments is improved, the problem that the gel is dissolved in the buffer solution due to the temperature rise of the buffer solution is avoided due to the introduction of the refrigerant, and the recovery purity of the DNA molecular fragments in the later period is ensured.

Claims (7)

1. A sliding connection type electrophoresis device for DNA purification is characterized in that: including cooling box, electrophoresis box and upper cover, place at least one electrophoresis box in the cooling box, set up a plurality of mutual isolation's electrophoresis cell in the electrophoresis box, the plate electrode that every electrophoresis cell both sides set up realizes the electricity with the binding post of the cooling box lateral wall setting of homonymy respectively through gliding mode and connects, and this binding post is connected with the power electricity, and lock upper cover on the cooling box remains the clearance between electrophoresis box and the cooling box, this clearance respectively with the coolant import and the coolant export intercommunication that cooling box surface set up.

2. The slide-on electrophoresis apparatus for DNA purification according to claim 1, wherein: set up a plurality of baffles in the electrophoresis box, this a plurality of baffles separate the electrophoresis box for a plurality of electrophoresis cell, and electrophoresis box bottom plate in every electrophoresis cell all is provided with the arch, is the gel dicing area on every arch, and the electrophoresis cell of protruding both sides is the plate electrode region.

3. The slide-contact electrophoresis apparatus for DNA purification according to claim 2, wherein: the inside board of bending that all sets up of electrophoresis box both sides lateral wall, the one side that the board lies in the electrophoresis box of bending is provided with the tip that extends to the electrophoresis tank in the same downwarping with electrophoresis cell quantity and forms the plate electrode, the opposite side tip of the board of bending with binding post electricity is connected.

4. The slide-on electrophoresis apparatus for DNA purification according to claim 3, wherein: the other side end of the bending plate is provided with a connecting contact, the outer side surface of the connecting contact is arc-shaped, and the arc-shaped protrudes to the outer side of the side wall of the electrophoresis box and is in sliding contact with a wiring terminal arranged in an alignment mode of the cooling box.

5. The slide-on electrophoresis apparatus for DNA purification according to claim 4, wherein: the outer edge of the wiring terminal is provided with external threads and is meshed with the surface of the side wall where the cooling box is aligned to the arc, the end part of the inner side of the wiring terminal is in sliding contact with the arc, the end part of the outer side of the wiring terminal is provided with a wire inserting hole and a fixing bolt, the wire inserting hole is used for inserting the end part of a cable connected with a power supply, and the fixing bolt is used for fixing the end part of the cable in the end part of the outer side of the wiring terminal.

6. The slide-on electrophoresis apparatus for DNA purification according to claim 4 or 5, wherein: the electrophoresis box is characterized in that at least one sealing ring is arranged at the upper end of the outer edge of the electrophoresis box from top to bottom, the outer edge of the electrophoresis box below the sealing ring is provided with a recess, the gap comprises the recess, the bottom surface of the electrophoresis box and the gap between the bottom surfaces inside the cooling box, the lower end of the surface of one side of the cooling box is provided with a refrigerant inlet, and the upper end of the surface of the other side of the cooling box is provided.

7. The slide-on electrophoresis apparatus for DNA purification according to claim 6, wherein: the gap also includes a space inside the protrusion.

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