CN211199188U

CN211199188U - DNA gel electrophoresis device convenient to use

Info

Publication number: CN211199188U
Application number: CN201921348567.2U
Authority: CN
Inventors: 杨冰; 杨博然; 闫亮亮; 李文佳
Original assignee: Tianjin Prius Biotechnology Co ltd
Current assignee: Tianjin Prius Biotechnology Co ltd
Priority date: 2019-08-20
Filing date: 2019-08-20
Publication date: 2020-08-07
Anticipated expiration: 2029-08-20

Abstract

The utility model relates to a DNA gel electrophoresis apparatus convenient to use, a plurality of electrophoresis cell of spaced placing in the end box, the inside plate electrode that sets up in every electrophoresis cell both sides is connected with the binding post electricity that sets up with the end box lateral wall of homonymy respectively, and this binding post is connected with the power electricity, lock upper cover on the end box. The utility model discloses in, a plurality of electrophoresis cell in the end box can once only a large amount of DNA molecular fragment's separation of carrying on, and the upper cover lock is convenient in addition, is provided with temperature sensor in gel tray district in addition, carries out the temperature measurement in this region by it, avoids the impurity that this department's buffer solution high temperature leads to the agarose gel's melting production.

Description

DNA gel electrophoresis device convenient to use

Technical Field

The utility model belongs to the technical field of electrophoresis equipment institutional advancement, especially a DNA gel electrophoresis apparatus convenient to use.

Background

DNA gel electrophoresis is a commonly used DNA separation technique that uses an apparatus configuration that is: including the electrophoresis cell, gel tray and power, set up an electrode plate respectively in the both sides of electrophoresis cell, the middle part of electrophoresis cell sets up a arch, place a plurality of gel tray on the arch, place agarose gel and DNA sample in the gel tray, put into the buffer solution of sufficient volume in the electrophoresis cell, make its gel surface that submerges the gel tray, then make the DNA molecule on the gel remove to anodal direction through the electric field between two electrode plates, because the molecule of different DNA molecule fragments is different with the configuration, the translation rate in the electric field is different, from this separate out different DNA molecule fragments. The both sides of electrophoresis cell set up a contact pin respectively, and the plate electrode of homonymy is connected to every contact pin lower extreme, and in the upper end of every contact pin imbeds the socket that the upper cover counterpointed the setting behind electrophoresis cell lock upper cover, two sockets of upper cover both sides were connected the anodal of power and the binding post of negative pole respectively. Because the contact pin need insert and imbed in the trompil of socket counterpoint, so it is not very convenient when actual lock, moreover, the electrophoresis cell that uses at present only can place two gel trays, and quantity is less, uses when can not satisfying a large amount of separation DNA molecule fragments, leads to the decline of experimental efficiency.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to overcome prior art's not enough, provide and use a convenient to use's DNA gel electrophoresis apparatus with a plurality of swimming pool integrations together and with the power wiring convenience, have the temperature measurement function.

The utility model adopts the technical proposal that:

a convenient DNA gel electrophoresis device, characterized in that: including end box, electrophoresis cell and upper cover, a plurality of electrophoresis cells are placed to the spaced in the end box, and the inside plate electrode that sets up in every electrophoresis cell both sides is connected with the binding post electricity that sets up with the end box lateral wall of homonymy respectively, and this binding post is connected with the power electricity, lock upper cover on the end box.

And moreover, a bending plate is arranged inside the side walls of the two sides of each electrophoresis cell, one end part of the bending plate, which is positioned in each electrophoresis cell, is bent downwards to extend and form an electrode plate, and the other end part of the bending plate is electrically connected with the wiring terminal.

And moreover, the outer surface of the side wall of the electrophoresis cell which is aligned with the upper end of the bending plate is provided with a bulge, the lower end of the bulge is embedded into a groove which is formed by aligning the bottom box, the end part of the other side of the bending plate extends downwards from the bottom surface of the bulge and contacts with the upper end of a connecting plate which is arranged on the bottom surface of the groove, and the lower end of the connecting plate is electrically connected with the wiring terminal.

Furthermore, the outer edge of the wiring terminal is provided with external threads and is meshed with the surface of the side wall where the bottom box and the wiring board are aligned, the end part of the inner side of the wiring terminal is in contact with the wiring board, the end part of the outer side of the wiring terminal is provided with a wire inserting hole and a fixing bolt, the wire inserting hole is used for inserting the end part of a cable connected with a power supply, and the fixing bolt is used for fixing the end part of the cable in the end part of the outer side.

Furthermore, a plurality of partition plates are arranged in the bottom box at intervals, and the plurality of partition plates separate a plurality of electrophoretic pools placed in the bottom box.

Furthermore, a bulge is arranged in the middle of the electrophoretic pool, and two vertical plates are respectively arranged on the bulge, which are close to two edges of two sides of the electrophoretic pool; the area of the electrophoresis cell at the two sides of the bulge is a polar plate area, and the area between two vertical plates at the upper end of the bulge is a gel tray area.

Furthermore, a plurality of temperature sensors are arranged on the upper cover in alignment with each electrophoresis cell, the lower end of each temperature sensor extends below the liquid level of the buffer solution in the gel tray area, and the output ends of the plurality of temperature sensors are connected with a socket arranged on the upper cover.

Furthermore, the inner edge of the upper cover is provided with convex ribs or spaced convex blocks, and the convex ribs or spaced convex blocks are pressed on the upper end surface of the bottom box after the upper cover is buckled on the bottom box; the lower end edge of the upper cover shields the wiring terminal.

The utility model has the advantages that:

the utility model discloses in, the spaced sets up a plurality of swimming pools in the end box, the opposite side tip of the inside portion of bending that sets up in every swimming pool both sides contacts with the inside wiring board upper end that counterpoints the setting of end box lateral wall, the wiring board lower extreme contacts with the binding post of end box lateral wall outside surface, realized the inside plate electrode of swimming pool and binding post's electricity from this and connected, during the use directly put into the end box with the swimming pool can, and the upper cover only is used for the lock on the end box and its lower extreme edge shelters from some binding posts. Through the cooperation of each above-mentioned structure, a plurality of electrophoresis cell in the end box can once only carry out the separation of DNA molecular fragment a large amount, and the upper cover closure is convenient in addition, is provided with temperature sensor in gel tray district in addition, carries out the temperature measurement in this region by it, avoids the impurity that the melting of agarose gel that this department's buffer solution temperature too high leads to produces.

Drawings

FIG. 1 is a schematic structural view of the present invention (with the upper cover removed);

FIG. 2 is a bottom view of FIG. 1;

FIG. 3 is a schematic view of the structure of FIG. 1 with all of the electrophoresis cells removed;

FIG. 4 is a left side view of FIG. 2;

FIG. 5 is an enlarged cross-sectional view of FIG. 4;

FIG. 6 is an enlarged view of section I of FIG. 5;

FIG. 7 is a schematic view of the structure of an electrode plate;

FIG. 8 is a schematic view of the structure of FIG. 2 after it has been snapped onto the lid;

fig. 9 is a state diagram of using two electrophoresis cells.

Detailed Description

The present invention is further illustrated by the following examples, which are intended to be illustrative, not limiting and are not intended to limit the scope of the invention.

The utility model provides a DNA gel electrophoresis device convenient to use, as shown in fig. 1 ~ 9, the utility model discloses an innovation lies in: including end box 1, electrophoresis cell 10 and upper cover 28, a plurality of electrophoresis cells are placed to the spaced in the end box, and the inside plate electrode 4 that sets up in every electrophoresis cell both sides is connected with the binding post 2 electricity that sets up with the end box lateral wall of homonymy respectively, and this binding post is connected with the power electricity, lock upper cover on the end box.

In this embodiment, a bending plate 16 is arranged inside the side walls of the two sides of each electrophoresis cell, one side end of the bending plate located in the electrophoresis cell is bent downwards to extend and form an electrode plate, and the other side end 21 of the bending plate is electrically connected with a connecting terminal. The preferred structure is: set up protruding 3 with the electrophoresis cell lateral wall surface of bending the board upper end counterpoint, this protruding lower extreme embedding end box is counterpointed in the recess 13 that sets up, and the opposite side tip of the board of bending contacts from protruding bottom surface downwardly extending and with the connecting plate 15 upper end that the bottom surface of groove set up, and this connecting plate lower extreme 26 is connected with the binding post electricity.

The outer edge of a convex ring 23 at the lower end of the wiring terminal is provided with external threads and is meshed and connected with the surface of the side wall of the bottom box aligned with the wiring board, the end part 22 at the inner side of the wiring terminal is contacted with the wiring board, the end part at the outer side of the wiring terminal is provided with a wiring hole 24 and a fixing bolt 12 as shown in figure 6, the wiring hole is used for inserting the end part of a cable connected with a power supply, and the fixing bolt is used for fixing the end part of the cable in the end.

The wire insertion hole and the fixing bolt may also take a structure as shown in fig. 3, i.e., the fixing bolt 12 is disposed at the side of the end portion outside the terminal, and the wire insertion hole 24 is disposed at the end face of the end portion outside the terminal.

A plurality of partition plates 11 are arranged in the bottom box at intervals, the interior of the bottom box is divided into a plurality of spaces 14 by the partition plates, an electrophoresis cell can be placed in each space, and different electrophoresis cells are separated by the partition plates.

The middle part of the electrophoresis cell is provided with a bulge 19, and two edges of the bulge, which are close to two sides of the electrophoresis cell, are respectively provided with a vertical plate 17; the areas of the electrophoresis cell at the two sides of the bulge are

polar plate areas

7 and 9, the area between two vertical plates at the upper end of the bulge is a gel tray area 5, and a gel tray 20 is placed in the gel tray area.

The electrode plate structure in the electrophoresis cell is shown in fig. 7, the upper end of the electrode plate structure extends into the protrusion 6 aligned with the side wall of the electrophoresis cell and is integrally connected with the bending plate, and the lower end of the electrode plate structure is arranged into a transverse bending structure 27.

The upper cover is provided with a plurality of temperature sensors 18 which are aligned with each electrophoresis cell, the lower end of each temperature sensor extends into the lower part of the liquid level of the buffer solution in the gel tray area, the output ends of the plurality of temperature sensors are connected with the wire row 29 which is arranged on the upper cover, all the wire rows are connected with the socket 30 which is arranged on the upper cover, a plug can be inserted into the socket, and the plug is connected with equipment which can receive and display data through a cable, an industrial personal computer, a temperature instrument, a computer and the like. The temperature sensor is used for detecting the temperature of the buffer solution in the gel tray area, the problem that the temperature of the buffer solution is too high due to the fact that the electrode plate is electrified is avoided, and when the temperature of the buffer solution is too high, the electrode plate is disconnected with the power supply.

The inner edge of the upper cover is provided with a convex rib 31 or a convex block at intervals, and the convex rib or the convex block at intervals is pressed on the upper end surface 8 of the bottom box after the upper cover is buckled on the bottom box. The lower end edge 32 of the upper cover shields the terminal.

The bottom box, the upper cover and the electrophoresis cell are made of transparent insulating materials, the bending plate is made of platinum materials, and the wiring board and the wiring terminal are made of copper materials.

The utility model discloses a use is shown in figure 9:

in this embodiment, only two electrophoretic cells are used, and are disposed in the two leftmost spaces of the bottom case.

All the wiring terminals are connected with a power supply through cables, a plug is inserted into the socket, and the other end of the plug is connected with the thermometer.

1. Agarose gel trays were placed into the gel tray area of the electrophoresis cell and DNA samples were placed, two agarose gel trays were placed in the gel tray area in each electrophoresis cell.

2. TAE buffer 25 was poured in, and then the power was turned on to apply power to both electrode plates.

3. After a period of time, the power supply is cut off, the gel tray is taken out for cutting, the DNA gel block needing further separation is taken out for subsequent elution treatment.

Claims

1. A convenient DNA gel electrophoresis device, characterized in that: including end box, electrophoresis cell and upper cover, a plurality of electrophoresis cells are placed to the spaced in the end box, and the inside plate electrode that sets up in every electrophoresis cell both sides is connected with the binding post electricity that sets up with the end box lateral wall of homonymy respectively, and this binding post is connected with the power electricity, lock upper cover on the end box.

2. The convenient DNA gel electrophoresis apparatus according to claim 1, wherein: the inside board that bends that sets up of lateral wall of the both sides of every electrophoresis cell, the board that bends is located one side tip in the electrophoresis cell and bends downwards and extend and form the plate electrode, the opposite side tip of the board of bending with binding post electricity is connected.

3. The convenient DNA gel electrophoresis apparatus according to claim 2, wherein: set up the arch with the electrophoresis cell lateral wall surface of bending board upper end counterpoint, this protruding lower extreme embedding end box is counterpointed in the recess that sets up, and the opposite side tip of the board of bending contacts from protruding bottom surface downwardly extending and the connecting plate upper end that sets up with the bottom surface of groove, this connecting plate lower extreme with the binding post electricity is connected.

4. A convenient DNA gel electrophoresis apparatus as claimed in claim 3 wherein: the outer edge of the wiring terminal is provided with external threads and is connected to the surface of the side wall, aligned with the wiring board, of the bottom box in a meshed mode, the end portion of the inner side of the wiring terminal is in contact with the wiring board, the end portion of the outer side of the wiring terminal is provided with a wire inserting hole and a fixing bolt, the wire inserting hole is used for inserting the end portion of a cable connected with a power supply, and the fixing bolt is used for fixing the end portion of the cable in the end.

5. The convenient DNA gel electrophoresis apparatus according to claim 4, wherein: a plurality of partition plates are arranged in the bottom box at intervals, and the plurality of partition plates separate a plurality of electrophoresis cells placed in the bottom box.

6. A convenient DNA gel electrophoresis device according to claim 1 or 2 or 3 or 4 or 5 wherein: the middle part of the electrophoresis cell is provided with a bulge, and two vertical plates are respectively arranged on the two edges of the bulge, which are close to the two sides of the electrophoresis cell; the area of the electrophoresis cell at the two sides of the bulge is a polar plate area, and the area between two vertical plates at the upper end of the bulge is a gel tray area.

7. The convenient DNA gel electrophoresis apparatus of claim 6, wherein: the upper cover is provided with a plurality of temperature sensors aligned with each electrophoresis cell, the lower end of each temperature sensor extends into the position below the liquid level of the buffer solution in the gel tray area, and the output ends of the plurality of temperature sensors are connected with the sockets arranged on the upper cover.

8. The convenient DNA gel electrophoresis apparatus of claim 7, wherein: the inner edge of the upper cover is provided with convex ribs or spaced convex blocks, and the convex ribs or spaced convex blocks are pressed on the upper end surface of the bottom box after the upper cover is buckled on the bottom box; the lower end edge of the upper cover shields the wiring terminal.