CN211170701U - Combined cerebrospinal fluid sequencing and collecting device - Google Patents
Combined cerebrospinal fluid sequencing and collecting device Download PDFInfo
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- CN211170701U CN211170701U CN201921706089.8U CN201921706089U CN211170701U CN 211170701 U CN211170701 U CN 211170701U CN 201921706089 U CN201921706089 U CN 201921706089U CN 211170701 U CN211170701 U CN 211170701U
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- cerebrospinal fluid
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Abstract
The utility model discloses a modular cerebrospinal fluid sequencing collection system, it includes: a base; the storage assembly is clamped on the base; the collection assembly comprises a plurality of collection test tubes and dropper bottles; which are respectively clamped on the corresponding base and the storage component; the utility model designs a cerebrospinal fluid collection system suitable for intracranial infectious disease, oncogene detect, it is through locating collection component activity deposit subassembly and base on, it enables the cerebrospinal fluid sample collection more standard, maintain the cerebrospinal fluid active ingredient, prolong the sample and preserve and transport the time to guarantee the stability of sequencing result.
Description
Technical Field
The utility model relates to the technical field of medical treatment, especially, relate to a combination formula cerebrospinal fluid sequencing collection system for intracranial infectious disease, oncogene detection.
Background
Central Nervous System (CNS) infection and meningeal cancer are still one of the most important fatal diseases in neurology, and are common and frequently encountered diseases in neurology, and due to the limitations of various traditional detection methods, over half of cases cannot be diagnosed clearly. With the development of molecular biology, gene sequencing has been an unprecedented technological innovation.
In recent years, the secondary sequencing of cerebrospinal fluid pathogens of patients with central nervous system infection, the genotyping of cerebrospinal fluid tumor cells of patients with meningeal cancer and the like have become hot spots of current cerebrospinal fluid diagnosis research.
However, clinicians still have the problems of irregular operation, easy contamination of samples, low nucleic acid extraction efficiency and the like in the cerebrospinal fluid collection process, and lack of quality control on how to store and transport the samples after collection, so that the gene sequencing result may have a significant bias.
In view of the above circumstances, there is no special device for collecting sequencing cerebrospinal fluid, so there are many inconveniences.
SUMMERY OF THE UTILITY MODEL
The utility model designs a cerebrospinal fluid collection system suitable for intracranial infectious disease, oncogene detect, it is through locating collection component activity deposit subassembly and base on, it enables the cerebrospinal fluid sample collection more standard, maintain the cerebrospinal fluid active ingredient, prolong the sample and preserve and transport the time to guarantee the stability of sequencing result.
In order to achieve the above object, the present invention mainly provides the following technical solutions: a combined cerebrospinal fluid sequencing collection device, comprising:
a base;
the storage assembly is clamped on the base;
the collection assembly comprises a plurality of collection test tubes and dropper bottles; which are respectively clamped in the corresponding base and the storage assembly.
The combined cerebrospinal fluid sequencing and collecting device comprises 3 collecting test tubes, namely a first test tube, a second test tube and a third test tube, wherein the 3 collecting test tubes are 3ml collecting test tubes;
adding DNA enzyme and RNA enzyme into the first test tube and the third test tube for pretreatment;
and adding a cerebrospinal fluid protective agent into the second test tube, wherein the cerebrospinal fluid protective agent comprises an anticoagulant K3 EDTA, a preservative and guanidinium isothiocyanate or β -mercaptoethanol.
The combined cerebrospinal fluid sequencing and collecting device comprises 3 collecting test tubes, a screw test tube, a test tube cap and a label attached to the collecting test tube, wherein the screw test tube is connected with the test tube cap;
the corresponding labels on the test tubes are respectively: primary-pathogen sequencing, secondary-tumor sequencing, tertiary-negative control;
the color of the test tube cap corresponding to each test tube is yellow, purple and green.
The combined cerebrospinal fluid sequencing and collecting device comprises a dropping bottle, a sampling tube and a sampling tube, wherein the dropping bottle is a 1-tube 5ml glass dropping bottle; sterile water is arranged in the dropper bottle, and the sterile water in the glass dropper bottle is transferred to the test tube III after the cerebrospinal fluid is collected.
The combined cerebrospinal fluid sequencing and collecting device comprises a base, a first base, a second base, a dropping bottle, a first buffer and a second buffer, wherein the base is L-shaped and comprises the first base and the second base;
the second base station is used for accommodating the storage table of the storage assembly.
The combined cerebrospinal fluid sequencing and collecting device comprises a storage table, a storage assembly, a sequencing module and a sequencing module, wherein the storage assembly comprises a storage table and a cover body;
one side surface of the cover body is provided with an opening at one side; grooves corresponding to the raised lines on the inner wall of the cover body are formed in the outer wall of the storage table; the storage table slides out of the cover body along one end of the side opening;
the storage table is movably connected with the second base table;
the side opening is provided with a side flip cover, the top end of the side flip cover is connected with the top wall of the cover body through a hinge, and the bottom end of the side flip cover is connected with the bottom wall of the cover body through a lock catch.
The combined cerebrospinal fluid sequencing and collecting device comprises a storage table, a positioning table and a sequencing and collecting device, wherein the storage table comprises an upper fixing plate, a lower fixing plate and a side plate;
the upper fixing plate is provided with a through hole, and the corresponding position of the lower fixing plate is arranged in the groove body;
the quantity of through-hole and cell body is three, is used for holding test tube one, test tube two and test tube three respectively.
The upper fixing plate and the lower fixing plate are made of silica gel.
The combined cerebrospinal fluid sequencing and collecting device comprises a plurality of through holes, wherein the through holes are provided with a plurality of through holes, and the through holes are provided with through holes.
The combined cerebrospinal fluid sequencing and collecting device comprises a first base platform, a second base platform, a storage platform, a magnetic piece and a magnetic piece, wherein the magnetic piece is arranged at the four corners of the second base platform, and the magnetic piece is arranged at the four corners of the second base platform.
The combined cerebrospinal fluid sequencing and collecting device is characterized in that the cover body is provided with a handle.
Compared with the prior art, the utility model discloses the beneficial effect who produces mainly embodies:
the utility model discloses a combined cerebrospinal fluid sequencing and collecting device, which arranges test tubes used for collecting and sequencing on corresponding storage components in sequence by arranging a base, a collecting component and a storage component; thereby further standardizing the collecting sequence of cerebrospinal fluid samples;
the utility model discloses a combined cerebrospinal fluid sequencing and collecting device, the base, the collecting component and the storing component are arranged in a combined way, and the base, the collecting component and the storing component can be conveniently detached and used or combined for use, so as to meet the requirements of collecting, storing and carrying of medical staff;
the utility model discloses a combined type cerebrospinal fluid sequencing and collecting device, three test tubes are distinguished obviously through the difference of labels and test tube caps, so that the effective collection and inspection of cerebrospinal fluid are effectively ensured, and the problem that the cerebrospinal fluid is polluted is avoided;
the utility model discloses a modular cerebrospinal fluid sequencing collection system, the standardized collection of effective standard cerebrospinal fluid gene sequencing sample, the problem of prevention sample pollution takes place.
The foregoing description is only an overview of the technical solutions of the present invention, and in order to clearly understand the technical means of the present invention and implement the technical means according to the content of the description, the following detailed description of the preferred embodiments of the present invention is given with reference to the accompanying drawings.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
Fig. 1 is a schematic structural diagram of a combined cerebrospinal fluid sequencing and collecting device of the present invention;
fig. 2 is a schematic structural view of the storage assembly of the present invention;
fig. 3 is a schematic structural view of the base of the present invention;
fig. 4 is a schematic structural diagram of the cover body of the present invention;
fig. 5 is a schematic structural diagram of the collecting assembly of the present invention;
description of reference numerals:
1. base 11, first base 12, second base
13. Accommodating groove 14, groove
2. Storage unit 21, storage table 22, and cover body
23. Groove 24, convex strip 25 and side turnover cover
26. Hinge 27, lock catch 211 and upper fixing plate
212. Lower fixing plate 213, side plate 214, and through hole
215. Trough 216, waterproof sticker 28, magnetism piece of inhaling
29. Lifting handle
3. Collection assembly 31, collection test tube 32 and dropper bottle
33. Test tube I34, test tube II 35 and test tube III
311. Screw test tube 312, test tube cap 313 and label
314. Scale value
Detailed Description
To further illustrate the technical means and effects of the present invention for achieving the objects of the present invention, the following detailed description will be given with reference to the accompanying drawings and preferred embodiments of the present invention for the specific embodiments, structures, features and effects of the combined cerebrospinal fluid sequencing and collecting device according to the present invention.
As shown in fig. 1, fig. 2 and fig. 5, the utility model discloses a combined cerebrospinal fluid sequencing and collecting device, it includes:
a base 1;
the storage assembly 2 is clamped on the base 1;
a collection assembly 3 comprising a plurality of collection tubes 31 and dropper bottles 32; which are respectively clamped in the corresponding base 1 and the storage assembly 2.
According to the combined cerebrospinal fluid sequencing and collecting device, the base 1, the collecting assemblies 3 and the storage assemblies 2 are arranged, and test tubes used for collecting and sequencing are placed on the corresponding storage assemblies 2 in sequence; thereby further standardizing the collecting sequence of cerebrospinal fluid samples;
the base 1 and the storage assembly 2 in the application can be reused for the second time after being sterilized, and the acquisition assembly 3 is disposable; it is used for collecting cerebrospinal fluid for intracranial infectious diseases and tumor gene sequencing.
According to the combined cerebrospinal fluid sequencing and collecting device, the base 1, the collecting assemblies 3 and the storage assemblies 2 are arranged, and test tubes used for collecting and sequencing are placed on the corresponding storage assemblies 2 in sequence; thereby further standardizing the collecting sequence of cerebrospinal fluid samples;
as shown in FIGS. 1 and 5, the utility model discloses a combined type cerebrospinal fluid sequencing collection device, collection test tube 31 includes 3 collection test tubes 31 of 3ml, and it is test tube 33, test tube two 34 and test tube three 35 respectively, add DNase and RNase preliminary treatment in test tube one 33 and test tube three 35, add cerebrospinal fluid protective agent in test tube two 34, cerebrospinal fluid protective agent includes anticoagulant K3 EDTA, antiseptic and guanidinium isothiocyanate or β -mercaptoethanol.
As shown in fig. 5, in the combined cerebrospinal fluid sequencing and collecting device of the present invention, 3 collecting test tubes 31 are respectively composed of a screw test tube 311, a test tube cap 312 and a label 313 attached to the collecting test tube 31; the corresponding labels 313 on each test tube are: primary-pathogen sequencing, secondary-tumor sequencing, tertiary-negative control; the color of the cap 312 for each test tube is yellow, purple and green.
As shown in fig. 5, in the combined cerebrospinal fluid sequencing and collecting device of the present invention, the dropping bottle 32 is a 1-piece 5ml glass dropping bottle 32; sterile water is arranged in the dropper bottle 32, and the sterile water in the glass dropper bottle 32 is transferred to the third test tube 35 after cerebrospinal fluid collection.
The combined cerebrospinal fluid sequencing and collecting device comprises collecting test tubes 31, wherein each collecting test tube 31 consists of a screw test tube 311, a test tube cap 312 and a label 313 attached to the collecting test tube 31;
each test tube is marked with 3ml scale, and the label 313 is distinguished by a serial number and a detection item: wherein:
the label 313 corresponding to test tube one 33 is used for pathogen sequencing;
the label 313 corresponding to tube two 34 is tumor sequencing;
label 313 corresponding to test tube three 35 is a negative control;
in addition to the above-mentioned label 313 distinction it is further distinguished by the color of the test tube cap 312: the color of the cap 312 for each test tube is yellow, purple and green.
After collecting cerebrospinal fluid, pretreating the cerebrospinal fluid in a first test tube 33 and a third test tube 35 by DNase and RNase, adding a special cerebrospinal fluid protective agent into a second test tube 34, wherein the cerebrospinal fluid protective agent is an anticoagulant K3 EDTA, a preservative and other nucleic acid stabilizers, and the nucleic acid stabilizers are guanidinium isothiocyanate or β -mercaptoethanol;
it should be noted that, all the 3 collection test tubes 31 are processed in vacuum or non-vacuum; the collection test tube 31 is a screw glass test tube, and the test tube cap 312 is made of plastic material; the scale values 314 are arranged on the collecting test tubes 31.
The dropper bottle 32 consists of a medical grade glass dropper, a polypropylene plastic cover, a medical grade glass bottle and a nitrile rubber head.
As shown in fig. 3, the base 1 of the combined cerebrospinal fluid sequencing and collecting device of the present invention is L-shaped, and includes a first base 11 and a second base 12, wherein the first base 11 is provided with a receiving groove 13 for receiving the dropper bottle 32 therein, and the second base 12 is used for receiving the storage table 21 of the storage assembly 2.
As shown in fig. 2 and fig. 4, the storage assembly 2 of the combined cerebrospinal fluid sequencing and collecting device of the present invention includes a storage table 21 and a cover 22; one side surface of the cover body 22 is provided with an opening; a groove 23 corresponding to the raised line 24 on the inner wall of the cover body 22 is arranged on the outer wall of the storage table 21; the storage table 21 slides out of the cover 22 along one end of the side opening; the storage table 21 is movably connected with the second base station 12; the side opening is provided with a side flip cover 25, the top end of the side flip cover 25 is connected with the top wall of the cover body 22 through a hinge 26, and the bottom end of the side flip cover 25 is connected with the bottom wall of the cover body 22 through a lock catch 27.
As shown in fig. 2, the storage table 21 of the combined cerebrospinal fluid sequencing and collecting device of the present invention includes an upper fixing plate 211, a lower fixing plate 212, and a side plate 213; a through hole 214 is formed in the upper fixing plate 211, and a through hole 215 is formed in a position corresponding to the lower fixing plate 212; the number of the through holes 214 and 215 is three, and the through holes are used for accommodating a test tube I33, a test tube II 34 and a test tube III 35 respectively; the upper fixing plate 211 and the lower fixing plate 212 are made of silicone.
The utility model discloses a combined cerebrospinal fluid sequencing and collecting device, which is provided with a base 1, a storage component 2 and a collecting component 3, wherein the base 1, the storage component 2 and the collecting component 3 can be combined into a whole when the three are used at the same time, so that the collecting, placing and storing operations of cerebrospinal fluid can be facilitated;
the storage table 21 can be separated from the cover body 22 of the storage assembly 2, and is fixed on the second base table 12 of the base 1 after being separated, so that cerebrospinal fluid collection and additive addition can be facilitated, and the storage table 21 and the cover body 22 can be combined to achieve the purposes of storage and carrying.
Each test tube is inserted into the corresponding through hole 214, and the bottom of each test tube is arranged in the through hole 215 of the lower fixing plate 212, so that the experimenter can observe the cerebrospinal fluid in the test tube from the position between the storage tables 21.
As shown in fig. 2, in the combined cerebrospinal fluid sequencing and collecting device of the present invention, a waterproof adhesive paper 216 corresponding to the color of the test tube cap 312 is attached to each through hole 214; the laboratory staff of further being convenient for correspondingly places each and gathers test tube 31.
As shown in fig. 2, in the combined cerebrospinal fluid sequencing and collecting device of the present invention, the bottom wall of the storage platform 21 is magnetically connected to the second base station 12 through a magnetic attraction member 28 disposed at four corners; the two are attracted by a certain magnetic force, so that the problem of movement of the storage table 21 can be prevented.
As shown in fig. 4, the utility model discloses a modular cerebrospinal fluid sequencing and collecting device, be equipped with handle 29 on the cover body 22, can be convenient for the experimenter to carry this and deposit subassembly 2.
A modular cerebrospinal fluid sequencing collection device of an embodiment of the present application, in-process of its use:
firstly, placing the storage assembly 2 on the second base station 12, and sliding the storage table 21 out of the cover body 22 to place the storage table 21 on the second base station 12;
the experimenter puts 3 collection test tubes 31 into the corresponding through holes 214 on the storage table 21 in sequence according to the difference of the labels 313; then, a dropper bottle 32 storing 3ml of sterile liquid is placed in the accommodating groove 13 on the first base platform 11;
in the collecting process, cerebrospinal fluid is sequentially injected into each collecting test tube 31, the cerebrospinal fluid in the first test tube 33 and the cerebrospinal fluid in the third test tube 35 are pretreated by DNase and RNase, a special cerebrospinal fluid protective agent is added into the second test tube 34, the cerebrospinal fluid protective agent is an anticoagulant K3 EDTA, a preservative and other nucleic acid stabilizers, and the nucleic acid stabilizers are guanidinium isothiocyanate or β -mercaptoethanol, and then 3ml of sterile liquid is added into the third test tube 35;
after the operation is finished, the storage table 21 can be slid into the cover body 22, the side turnover cover 25 is turned down to be sealed in the cover body 22, and the storage assembly 2 is integrally inspected.
The present invention has been further described with reference to the embodiments, but the present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art.
Claims (10)
1. The utility model provides a modular cerebrospinal fluid sequencing collection system which characterized in that, it includes:
a base;
the storage assembly is clamped on the base;
the collection assembly comprises a plurality of collection test tubes and dropper bottles; which are respectively clamped in the corresponding base and the storage assembly.
2. The combined cerebrospinal fluid sequencing collection device of claim 1, wherein:
the collecting test tubes comprise 3 collecting test tubes of 3ml, namely a test tube I, a test tube II and a test tube III;
adding DNA enzyme and RNA enzyme into the first test tube and the third test tube for pretreatment;
and a cerebrospinal fluid protective agent is added into the second test tube.
3. The combined cerebrospinal fluid sequencing collection device of claim 2, wherein the cerebrospinal fluid sequencing collection device comprises a probe for detecting cerebrospinal fluid flow
The 3 collecting test tubes are all composed of screw test tubes, test tube caps and labels attached to the collecting test tubes;
the corresponding labels on the test tubes are respectively: primary-pathogen sequencing, secondary-tumor sequencing, tertiary-negative control;
the color of the test tube cap corresponding to each test tube is yellow, purple and green.
4. The combined cerebrospinal fluid sequencing collection device of claim 2, wherein:
the dropping bottle is a 1-piece 5ml glass dropping bottle; sterile water is arranged in the dropper bottle, and the sterile water in the glass dropper bottle is transferred to the test tube III after the cerebrospinal fluid is collected.
5. The combined cerebrospinal fluid sequencing collection device of claim 3, wherein:
the base is L-shaped and comprises a first base station and a second base station, wherein the first base station is provided with a containing groove for placing the dropper bottle;
the second base station is used for accommodating the storage table of the storage assembly.
6. The combined cerebrospinal fluid sequencing collection device of claim 5, wherein:
the storage assembly comprises a storage table and a cover body;
one side surface of the cover body is provided with an opening at one side; grooves corresponding to the raised lines on the inner wall of the cover body are formed in the outer wall of the storage table; the storage table slides out of the cover body along one end of the side opening;
the storage table is movably connected with the second base table;
the side opening is provided with a side flip cover, the top end of the side flip cover is connected with the top wall of the cover body through a hinge, and the bottom end of the side flip cover is connected with the bottom wall of the cover body through a lock catch.
7. The combined cerebrospinal fluid sequencing collection device of claim 6, wherein:
the storage table comprises an upper fixing plate, a lower fixing plate and a side plate;
the upper fixing plate is provided with a through hole, and the corresponding position of the lower fixing plate is arranged in the groove body;
the number of the through holes and the number of the groove bodies are three, and the through holes and the groove bodies are respectively used for accommodating a first test tube, a second test tube and a third test tube;
the upper fixing plate and the lower fixing plate are made of silica gel.
8. The combined cerebrospinal fluid sequencing collection device of claim 7, wherein:
waterproof stickers corresponding to the colors of the test tube caps are attached to the through holes.
9. The combined cerebrospinal fluid sequencing collection device of claim 8, wherein:
the bottom wall of the storage table is magnetically connected with the second base platform through magnetic pieces arranged at four corners.
10. The combined cerebrospinal fluid sequencing collection device of claim 6, wherein:
the cover body is provided with a handle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921706089.8U CN211170701U (en) | 2019-10-12 | 2019-10-12 | Combined cerebrospinal fluid sequencing and collecting device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921706089.8U CN211170701U (en) | 2019-10-12 | 2019-10-12 | Combined cerebrospinal fluid sequencing and collecting device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211170701U true CN211170701U (en) | 2020-08-04 |
Family
ID=71804204
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921706089.8U Expired - Fee Related CN211170701U (en) | 2019-10-12 | 2019-10-12 | Combined cerebrospinal fluid sequencing and collecting device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211170701U (en) |
-
2019
- 2019-10-12 CN CN201921706089.8U patent/CN211170701U/en not_active Expired - Fee Related
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200804 Termination date: 20211012 |