CN211111961U

CN211111961U - Cell thawing device

Info

Publication number: CN211111961U
Application number: CN201921854415.XU
Authority: CN
Inventors: 张永慧; 谢玉清
Original assignee: Chongqing Three Gorges Medical College
Current assignee: Chongqing Three Gorges Medical College
Priority date: 2019-10-30
Filing date: 2019-10-30
Publication date: 2020-07-28
Anticipated expiration: 2029-10-30

Abstract

The utility model belongs to the field of experimental articles, and relates to a cell thawing device, which comprises a main body, a water filling port and a water outlet which are arranged on the main body, a test tube placing tray arranged at the top of the main body, and a timing and constant temperature heating module arranged at the bottom of the main body; the test tube placing plate is provided with a plurality of via holes for fixing the test tubes. The utility model is simple in operation, the cell damage rate is low, ensures the effect of unfreezing through electronic control's heating temperature and heat time, plays disinfection's effect through the ultraviolet irradiation lamp, reduces the cell and unfreezes the emergence that the in-process pollutes or dies.

Description

Cell thawing device

Technical Field

The utility model belongs to the field of experimental articles, a cell thawing apparatus is related to.

Background

In addition to the optimal freezing speed, the appropriate cryoprotectant and freezing temperature, the cryopreservation of the in vitro culture also needs to have the optimal rewarming speed during the recovery, so that the optimal cryopreservation effect can be ensured finally. In most cell recovery processes, the freezing tube is taken out of liquid nitrogen, immediately put into a water bath kettle at 37 ℃, manually and slightly shaken, and the liquid is taken out after being melted. However, most steps of the process are manually controlled, and the process has various instabilities (temperature conditions, sterile environment, water bath time and the like), is easy to cause cell pollution or cell death caused by improper cell recovery.

SUMMERY OF THE UTILITY MODEL

In view of this, the present invention provides a cell thawing apparatus, which can simultaneously thaw a large number of cells, reduce cell contamination, and eliminate unstable factors in the conventional manual operation.

In order to achieve the above purpose, the utility model provides a following technical scheme:

a cell thawing device is characterized by comprising a main body, a water filling port and a water outlet which are arranged on the main body, a test tube placing disc arranged at the top of the main body, and a timing and constant-temperature heating module arranged at the bottom of the main body; the test tube placing plate is provided with a plurality of via holes for fixing the test tubes.

Optionally, the test tube placing tray further comprises a tray cover which covers the test tube placing tray.

Optionally, the main body is further provided with an observation window, and the observation window is made of transparent materials.

Optionally, an ultraviolet radiation lamp is arranged in the main body.

Optionally, still include the test tube mount, set up on the interior ground of main part for fixed test tube.

Optionally, a time button and a temperature button are arranged on the timing and constant temperature heating module and used for adjusting the heating temperature and the heating time.

Optionally, the timed and fixed-temperature heating module is detachably arranged at the bottom of the main body.

Optionally, the water injection port is arranged at the top of the main body, and the water outlet is arranged at the bottom of the main body.

Optionally, the timing and constant-temperature heating module is powered by an external power supply.

Optionally, a support column and a blade rotatably arranged on the support column are arranged on the inner bottom surface of the main body.

The beneficial effects of the utility model reside in that:

the utility model is simple in operation, the cell damage rate is low, ensures the effect of unfreezing through electronic control's heating temperature and heat time, plays disinfection's effect through the ultraviolet irradiation lamp, reduces the cell and unfreezes the emergence that the in-process pollutes or dies.

Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention. The objectives and other advantages of the invention may be realized and attained by the means of the instrumentalities and/or combinations particularly pointed out in the appended claims.

Drawings

For the purposes of promoting a better understanding of the objects, features and advantages of the invention, reference will now be made to the following detailed description taken in conjunction with the accompanying drawings in which:

fig. 1 is a schematic view of the overall structure of the present invention.

Detailed Description

The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will be readily apparent to those skilled in the art from the disclosure herein. The present invention can also be implemented or applied through other different specific embodiments, and various details in the present specification can be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that the drawings provided in the following embodiments are only for illustrating the basic idea of the present invention, and the features in the following embodiments and examples may be combined with each other without conflict.

Wherein the showings are for the purpose of illustrating the invention only and not for the purpose of limiting the same, and in which there is shown by way of illustration only and not in any way limiting the scope of the invention; for a better understanding of the embodiments of the present invention, some parts of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted.

The same or similar reference numerals in the drawings of the embodiments of the present invention correspond to the same or similar parts; in the description of the present invention, it should be understood that if there are terms such as "upper", "lower", "left", "right", "front", "back", etc., indicating directions or positional relationships based on the directions or positional relationships shown in the drawings, it is only for convenience of description and simplification of description, but it is not intended to indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore, the terms describing the positional relationships in the drawings are only used for illustrative purposes and are not to be construed as limiting the present invention, and those skilled in the art can understand the specific meanings of the terms according to specific situations.

Referring to FIG. 1, the reference numbers in the drawings denote: the device comprises a main body 1, a water filling port 2, an ultraviolet irradiation lamp 3, a timing and constant-temperature heating module 4, a test tube placing plate 5, a plate cover 6, a test tube fixing frame 7, a water outlet 8, an observation window 9 and blades 10.

The utility model relates to a cell thawing device, which is characterized by comprising a main body 1, a water filling port 2 and a water outlet 8 which are arranged on the main body 1, a test tube placing tray 5 which is arranged at the top of the main body 1, and a timing and constant temperature heating module 4 which is arranged at the bottom of the main body 1; the test tube placing plate 5 is provided with a plurality of through holes for fixing the test tubes.

Optionally, the test tube placing tray also comprises a tray cover 6 which covers the test tube placing tray 5; the main body 1 is also provided with an observation window 9, and the observation window 9 is made of transparent materials; an ultraviolet irradiation lamp 3 is arranged in the main body 1; the test tube fixing frame 7 is arranged on the inner ground of the main body 1 and used for fixing a test tube; the timing and constant temperature heating module 4 is provided with a time button and a temperature button for adjusting the heating temperature and the heating time; the timing and constant-temperature heating module 4 is detachably arranged at the bottom of the main body 1; the water injection port 2 is arranged at the top of the main body 1, and the water outlet 8 is arranged at the bottom of the main body 1; the timing and constant temperature heating module 4 is powered by an external power supply; the inner bottom surface of the main body 1 is provided with a support column and blades 10 rotatably arranged on the support column.

Specifically, the utility model discloses two kinds of schemes of unfreezing can be provided:

scheme one

Thawing frozen cells in the test tubes by a water bath heat matching timing constant-temperature heating module 4, plugging a water outlet 8 tightly, injecting quantitative water through a water injection port 2, then placing a plurality of test tubes into a main body 1 through a test tube placing disc 5, circumferentially fixing by the test tube placing disc 5, fixing the bottom of the test tube by a test tube fixing frame 7, and slightly extending the tops of the test tubes out of the main body 1; after the time and the temperature are selected, the cell thawing condition is determined through the observation window 9, and the ultraviolet irradiation lamp 3 plays a role in disinfection; after the unfreezing is finished, the tray cover 6 is covered to prevent ash from falling in the main body 1.

Scheme two

Carry out rotatory unfreezing through the circulation warm water, let in circulation warm water with water filling port 2 and delivery port 8, can select to let in constant temperature water, delivery port 8 in continuing to water filling port 2 and keep normally opening, through guaranteeing that

water filling port

2, 8 fluxes of delivery port are unanimous, guarantee that the inside water yield of main part 1 is steady. Under the circulation effect of rivers, drive blade 10 on the support column and produce the rotation, stir the interior liquid of main part 1, temperature transfer is more even.

The utility model is simple in operation, the cell damage rate is low, ensures the effect of unfreezing through electronic control's heating temperature and heat time, plays disinfection's effect through ultraviolet irradiation lamp 3, reduces the cell and unfreezes the emergence that the in-process pollutes or dies.

Finally, the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions, and all of them should be covered by the scope of the claims of the present invention.

Claims

1. A cell thawing device is characterized by comprising a main body, a water filling port and a water outlet which are arranged on the main body, a test tube placing disc arranged at the top of the main body, and a timing and constant-temperature heating module arranged at the bottom of the main body; the test tube placing plate is provided with a plurality of via holes for fixing the test tubes.

2. The cell thawing apparatus according to claim 1, further comprising a tray cover covering the tube placing tray.

3. The cell thawing apparatus according to claim 1, wherein said body further comprises an observation window, said observation window being made of a transparent material.

4. The cell thawing apparatus according to claim 1, wherein an ultraviolet irradiation lamp is provided in said main body.

5. The cell thawing apparatus according to claim 1, further comprising a test tube holder provided on an inner ground of the main body, for holding the test tube.

6. The cell thawing apparatus according to claim 1, wherein the timed and constant-temperature heating module is provided with a time button and a temperature button for adjusting a heating temperature and a heating time.

7. The cell thawing apparatus according to claim 1, wherein the timed, constant-temperature heating module is detachably provided at the bottom of the main body.

8. The cell thawing apparatus according to claim 1, wherein said water injection port is provided at a top of the main body, and said water outlet port is provided at a bottom of the main body.

9. The cell thawing apparatus according to claim 1, wherein the timed, constant temperature heating module is powered by an external power source.

10. The cell thawing apparatus according to claim 1, wherein a support column and a blade rotatably provided on the support column are provided on an inner bottom surface of the main body.