CN211094095U - A pushpin appearance electrode for recording of young mouse electroencephalogram - Google Patents
A pushpin appearance electrode for recording of young mouse electroencephalogram Download PDFInfo
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- CN211094095U CN211094095U CN201921548612.9U CN201921548612U CN211094095U CN 211094095 U CN211094095 U CN 211094095U CN 201921548612 U CN201921548612 U CN 201921548612U CN 211094095 U CN211094095 U CN 211094095U
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Abstract
The utility model discloses a thumbtack-like electrode for recording electroencephalograms of young mice, which has the technical proposal that the thumbtack-like electrode comprises a connecting plate and an electrode wire arranged in the middle of the connecting plate; the top end of the electrode wire penetrates through the connecting plate and is arranged at the lower end of the connecting plate, and a contact part is arranged at the joint of the electrode wire and the connecting plate; the top of the electrode wire is connected with a lead which is arranged at the upper end of the connecting plate; and the connecting plate is provided with a fixing unit. The method realizes rapid positioning and low-damage implantation of the brain electrode of the young mouse by low-trauma, auxiliary fixed accessory design and other methods.
Description
Technical Field
The invention relates to an electroencephalogram recording electrode, in particular to a pushpin-like electrode for recording electroencephalograms of young mice.
Background
The electroencephalogram of the young mouse is an important method for researching brain diseases of the infant such as human febrile convulsion, and the neuroelectric activity in the brain of the young mouse can be effectively recorded through electrode implantation, so that the electroencephalogram can be used for diagnosing diseases, evaluating the therapeutic action of medicines and researching the pathogenesis of the diseases. The rapid and effective counter electrode implantation can reduce the mortality rate of the young animals in the operation process, reduce the dosage of the animals and reduce the workload of experimenters.
The prior art has the following disadvantages: currently, most of the scalp is usually surgically isolated, a hole is drilled in the skull, and then an electrode is implanted and fixed by dental cement to a young mouse. However, the skull of the young mouse is very fragile and soft, the operation of drilling and the like is easy to cause large damage, the operation steps are more and time-consuming, and the death rate of the mouse is high. Moreover, because the conventional electrode has no supporting accessories, and the skull of the young mouse is very soft and cannot be fixed by an anchoring screw for assisting the electrode fixation, the conventional electrode has difficulty in positioning and infirm fixation in the implantation process due to insufficient support to a stress point, and is difficult to record permanently.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a pushpin-like electrode for recording electroencephalograms of a young mouse, which realizes quick positioning and implantation of the electrode into the brain of the young mouse with low damage by methods such as low trauma, design of auxiliary fixing accessories and the like.
In order to achieve the purpose, the invention provides the following technical scheme: a kind of thumb pin-like electrode used for electroencephalogram recording of young mice, including the connecting plate and electrode wire set up in the middle part of connecting plate; the top end of the electrode wire penetrates through the connecting plate and is arranged at the lower end of the connecting plate, and a contact part is arranged at the joint of the electrode wire and the connecting plate; the top of the electrode wire is connected with a lead which is arranged at the upper end of the connecting plate; and the connecting plate is provided with a fixing unit.
Through adopting above-mentioned technical scheme, directly set up the connecting plate on the mouse skull, the wire electrode of connecting plate lower extreme runs through the head entering detection area of mouse, realizes the position that awaits measuring of quick location brain electricity, has shortened the process time that the electrode directly implanted the mouse brain, has avoided arousing the unstability of mouse vital sign because of long-time operation to influence the numerical value of electroencephalogram record, fixed unit on the connecting plate sets up more convenient installation on the brain bag of mouse, and can accurate aim at corresponding position and detect.
The invention is further configured to: the electrode wire comprises a first electrode wire and a second electrode wire which are perpendicular to the connecting plate respectively, the first electrode wire and the second electrode wire are arranged in a manner of being symmetrical with the center of the connecting plate, and the distance between the contact part of the first electrode wire and the contact part of the second electrode wire is 0.5 mm.
Through adopting above-mentioned technical scheme, the first wire electrode and the second wire electrode that the symmetry set up can realize that record electrode (below wire electrode) and reference electrode (reference wire electrode) implant simultaneously to because reference electrode implantation is more shallow has reduced its injury to the brain.
The invention is further configured to: the fixing unit includes a plurality of through holes surrounding an outer side of the contact portion.
By adopting the technical scheme, the adhesive can be arranged in the through holes and used for fixing the connecting plate at the upper end of the skull of a mouse, the plurality of through holes can improve the adhesive contact surface, the stability is improved, and the position deviation of the wire electrode is prevented.
The invention is further configured to: the connecting plate is a circular connecting plate.
By adopting the technical scheme, the diameter of the circular connecting plate can be set to be about 3mm, and because the mice with similar body shapes for 11-13 days are selected, the circular shape and the diameter setting basically accord with the shapes and the sizes of the skull of the young mice, so that the electrode is conveniently and firmly fixed and has a certain skull protection effect.
The invention is further configured to: the through holes comprise three round holes which are equal to the distance between the centers of the connecting plates, the center of one round hole is positioned on the extension lines of the two contact parts, and the other two round holes are symmetrically arranged along the extension lines.
Through adopting above-mentioned technical scheme, the diameter of round hole can be according to the directness of circular connecting plate and set up, when the diameter of circular connecting plate sets up to 3mm, the diameter can set to 0.6mm, and the adhesive of being convenient for fixes the connecting plate on the skull, and the electrode connecting plate in 3 holes helps improving the accuracy of location, reduces electrode positioning electrode and calculates and operating time, and the electrode of prefabricated fixed interval can once implant recording electrode and reference electrode simultaneously. And can play the guard action to the mouse, not only increase the electrode fastness, help the electrode location moreover, improved the precision of final data.
The invention is further configured to: the length of the first electrode wire is longer than that of the second electrode wire.
By adopting the technical scheme, the first electrode wire is a longer metal electrode wire with an insulating layer and used for recording, and the second electrode wire is a shorter common electrode wire used as a reference, so that the transmission of electrical signals in the brain is realized.
The invention is further configured to: the length of the first electrode wire is 1-2 mm, and the length of the second electrode wire is 0.5-1 mm.
By adopting the technical scheme, the first electrode wire is used for recording the electrode, the length of the electrode wire is determined according to the position of electroencephalogram needing to be recorded, and the length can be adjusted according to specific experimental needs. The length is set so that the tip of the wire electrode can just reach the position to be recorded, and steps such as positioning and reading are omitted; the second wire electrode is arranged in a length such that the wire electrode enters the surface of the brain, but unnecessary damage to the brain is reduced.
The invention is further configured to: the first electrode wire is a metal electrode wire with an insulating layer.
By adopting the technical scheme, the metal electrode wire with the insulating layer has good bioelectricity, and the local electroencephalogram signal recording of the tip can be better realized through the insulating design.
The invention is further configured to: the connecting plate is a circuit board or a plastic plate.
By adopting the technical scheme, the circuit board and the plastic board are easy to obtain and prepare, the weight is light, the using amount of adhesives such as dental cement can be reduced, and a certain skull protection effect can be achieved. The material density is small, the weight is light, and the mouse burden is reduced. And the circuit board can be directly fixed by welding, and the installation is simple.
The invention is further configured to: the contact parts are all provided with adhesive layers.
Through adopting above-mentioned technical scheme, when the connecting plate is the plastic slab, utilize the viscose layer to be connected electrode wire and connecting plate fixedly, the viscidity of glue is strong, and the solidification speed is fast, and convenient safety is got up in the use, has improved the steadiness of whole device, has increased brain electricity measuring stability, has also improved the rate of utilization of device.
The invention has the following advantages:
1. the pushpin-like electrode adopted by the invention can realize rapid positioning of the position to be measured of the electroencephalogram, can overcome the defect of long operation time of electrode implantation in the prior art, and avoids unstable vital signs of mice, such as abnormal respiratory frequency and the like, caused by overlong operation time.
2. The drawing pin-like method adopted by the invention can improve the accuracy of brain positioning of the young mouse because the distance between the reference electrode and the recording electrode is fixed.
3. The connection mode of connecting the three holes of the connecting plate with the skull plays a role in protecting the young mouse, so that the firmness of the electrode is greatly improved, the holes formed in the connection mode are favorable for positioning the electrode, and the head wound of the mouse can be effectively protected; meanwhile, the device has small volume, is suitable for the head of a young mouse, and has small interference on the normal movement of the young mouse.
4. The connection mode of the connecting plate and the skull of the young mouse, the connection mode of the connecting plate and the electrode wire and the lead are stable, the stability of electroencephalogram measurement is improved, electroencephalogram data are recorded to be closer to the normal physiological activities of experimental animals, and secondary damage to brain tissues caused by instability of the lead is eliminated.
5. The drawing pin-like electroencephalogram recording device provided by the invention can be used for electroencephalogram recording and research on young mice by a simple method, and can be used for laying a cushion for subsequent research, thereby having important application value. The drawing pin-like electroencephalogram recording device provided by the invention can be used for electroencephalogram recording and research on young mice by a simple method, and can be used for laying a cushion for subsequent research, thereby having important application value.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic top view of the present invention;
in the figure: 1. a connecting plate; 2. a wire; 3. a contact portion; 4. a through hole; 5. a first wire electrode; 6. and a second wire electrode.
Detailed Description
Example 1
Referring to fig. 1 and 2, the pushpin-like electrode for recording electroencephalograms of young mice comprises a connecting plate 1 and an electrode wire arranged in the middle of the connecting plate 1; the top ends of the electrode wires penetrate through the connecting plate 1 and are fixed at the lower end of the connecting plate 1, the electrode wires comprise a first electrode wire 5 and a second electrode wire 6 which are perpendicular to the connecting plate 1 respectively, the first electrode wire 5 and the second electrode wire 6 are symmetrically arranged at the center of the connecting plate 1, the connecting parts of the first electrode wire 5 and the second electrode wire 6 and the connecting plate 1 are respectively provided with a contact part 3, the distance between the contact part 3 of the first electrode wire 5 and the contact part 3 of the second electrode wire 6 is 0.5mm, the connecting plate is fixed on the skull of a mouse, the invention generally selects mice with similar 11-13 celestial bodies, and the two electrode wires are symmetrically arranged about the midline of the brain bag of the mouse, so that experimental errors can be greatly reduced;
the top ends of the first electrode wire 5 and the second electrode wire 6 are respectively connected with a lead wire 2 and are connected in a welding mode, the two lead wires 2 are arranged at the upper end of a connecting plate 1, and the connecting plate 1 is used for fixing the positions of the electrode wires and the lead wires 2 to form an integral nail-like electrode structure; the length of first wire electrode 5 is longer than the length of second wire electrode 6, and first wire electrode 5 is the longer metal wire electrode that takes the insulating layer that is used for the record, and the second wire electrode is the ordinary wire electrode of shorter conduct reference, the longer metal wire electrode length that takes the insulating layer that is used for the record is 1mm, and the shorter ordinary wire electrode length that takes the reference is 0.5mm to realize the efferent of signal of telecommunication in the brain. The connecting plate can be a round plastic plate with certain hardness and the diameter of the connecting plate is 3mm, and because the shape of the connecting plate is similar to that of a mouse with the selected shape of 11-13 days, the round and diameter arrangement basically conforms to the shape and the size of the skull of the young mouse, so that the electrode can be firmly fixed conveniently and has certain skull protection effect. The second wire electrode 6 and the connection plate 1, and the first wire electrode 5 and the connection plate 1 are fixed by applying an adhesive of an adhesive layer to both contact portions 3.
A fixing unit is arranged on the connecting plate 1 and comprises a plurality of through holes 4 surrounding the outer side of the contact part 3, the through holes 4 comprise three round holes with the same distance with the circle center of the connecting plate 1, and the three round holes formed in the connecting plate 1 are convenient for an adhesive to fix the connecting plate 1 and the skull of the mouse; the diameters of the three round holes are all 0.6mm, the circle center of one round hole is positioned on the extension line of the two contact parts 3, and the other two round holes are symmetrically arranged along the extension line.
Example 2
Referring to fig. 1 and 2, the pushpin-like electrode for recording electroencephalograms of young mice comprises a connecting plate 1 and an electrode wire arranged in the middle of the connecting plate 1; the top ends of the electrode wires penetrate through the connecting plate 1 and are fixed at the lower end of the connecting plate 1, the electrode wires comprise a first electrode wire 5 and a second electrode wire 6 which are perpendicular to the connecting plate 1 respectively, the first electrode wire 5 and the second electrode wire 6 are symmetrically arranged at the center of the connecting plate 1, contact parts 3 are arranged at the connection parts of the first electrode wire 5 and the second electrode wire 6 and the connecting plate 1 respectively, the distance between the contact part 3 of the first electrode wire 5 and the contact part 3 of the second electrode wire 6 is 0.5mm, the invention generally selects mice with similar 11-13 celestial bodies, and the two electrode wires are symmetrically arranged about the center line of the brain bag of the mouse, so that experimental errors can be greatly reduced;
the top ends of the first electrode wire 5 and the second electrode wire 6 are respectively connected with a lead wire 2, the lead wires 2 are arranged at the upper end of a connecting plate 1, and the connecting plate 1 is used for fixing the positions of the electrode wires and the lead wires 2 to form an integral nail-like electrode structure; the length of the first electrode wire 5 is longer than that of the second electrode wire 6, the first electrode wire 5 is a longer metal electrode wire with an insulating layer and used for recording, the second electrode wire is a shorter common electrode wire used as a reference, the length of the longer metal electrode wire with the insulating layer and used for recording is 1.6mm, and the length of the shorter common electrode wire used as the reference is 0.8mm, so that the electrical signals in the brain are transmitted out; the connection can be set into a circular circuit board with certain hardness, and the first electrode wire 5, the second electrode wire 6, the circuit board and the lead 2 are welded together in a soldering tin welding mode; the connecting plate 1 is provided with a fixing unit, the fixing unit comprises a plurality of through holes 4 surrounding the outer side of the contact part 3 and used for fixing the electrode wires and the lead wires 2, the diameter of the connecting plate 1 is 3mm, and the shape and the diameter of the connecting plate are similar to those of a mouse in 11-13 days, so that the circular shape and the diameter are basically consistent with the shape and the size of the skull of the mouse, the electrode is conveniently and firmly fixed, and a certain skull protection effect is achieved. The second wire electrode 6 and the connection plate 1, and the first wire electrode 5 and the connection plate 1 are fixed by applying an adhesive of an adhesive layer to both contact portions 3.
A fixing unit is arranged on the connecting plate 1 and comprises a plurality of through holes 4 surrounding the outer side of the contact part 3, the through holes 4 comprise three round holes with the same distance with the circle center of the connecting plate 1, and the three round holes formed in the connecting plate 1 are convenient for an adhesive to fix the connecting plate 1 and the skull of the mouse; the diameters of the three round holes are all 0.6mm, the circle center of one round hole is positioned on the extension line of the two contact parts 3, and the other two round holes are symmetrically arranged along the extension line.
By adopting the technical scheme, the method has the advantages that,
1. according to the requirements, the parts of the first electrode wire 5 and the second electrode wire 6 which vertically extend downwards and exceed the connecting plate 1 are arranged in the deep brain area of the young mouse (taking the median association center or the primary society of the early mental trunk as an example, the front fontanel is 1.9mm behind, the side is 0.9mm, and the depth is 1.0-1.2 mm), and are fixed by dental cement through a three-round hole;
2. the two leads 2 are respectively connected and fixed with an electroencephalograph to record electroencephalograms.
The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments, and all technical solutions belonging to the idea of the present invention belong to the protection scope of the present invention. It should be noted that modifications and embellishments within the scope of the invention may occur to those skilled in the art without departing from the principle of the invention, and are considered to be within the scope of the invention.
Claims (10)
1. A pushpin-like electrode for electroencephalogram recording of a young mouse, characterized in that: comprises a connecting plate (1) and an electrode wire arranged in the middle of the connecting plate (1); the top end of the electrode wire penetrates through the connecting plate (1) and is arranged at the lower end of the connecting plate (1), and a contact part (3) is arranged at the connecting part of the electrode wire and the connecting plate (1); the top of the electrode wire is connected with a lead (2), and the lead (2) is arranged at the upper end of the connecting plate (1); the connecting plate (1) is provided with a fixing unit.
2. The pushpin-like electrode for electroencephalogram recording of a young mouse according to claim 1, wherein the wire electrode comprises a first wire electrode (5) and a second wire electrode (6) which are respectively perpendicular to the connecting plate (1), the first wire electrode (5) and the second wire electrode (6) are symmetrically arranged with respect to the center of the connecting plate (1), and the distance between the contact part (3) of the first wire electrode (5) and the contact part (3) of the second wire electrode (6) is 0.5 mm.
3. The pushpin-like electrode for electroencephalogram recording of a young mouse according to claim 2, characterized in that said fixing unit comprises a plurality of through holes (4) surrounding the outside of the contact portion (3).
4. The pushpin-like electrode for electroencephalogram recording of a young mouse according to claim 3, wherein said connection plate (1) is a circular connection plate.
5. The pushpin-like electrode for electroencephalogram recording of a young mouse according to claim 4, wherein the through hole (4) comprises three circular holes having a distance equal to the center of the connecting plate (1), wherein the center of one circular hole is located on the extension line of two contact parts (3), and the other two circular holes are symmetrically arranged with respect to the extension line.
6. The pushpin-like electrode for electroencephalogram recording of a young mouse according to claim 2, wherein the length of the first wire electrode (5) is longer than the length of the second wire electrode (6).
7. The pushpin-like electrode for electroencephalogram recording of a young mouse according to claim 6, wherein the length of the first wire electrode (5) is 1-2 mm, and the length of the second wire electrode (6) is 0.5-1 mm.
8. The pushpin-like electrode for electroencephalogram recording of a young mouse according to claim 2, wherein said first wire electrode (5) is a metal wire electrode with an insulating layer.
9. The pushpin-like electrode for electroencephalogram recording of a young mouse according to claim 1, wherein said connection board (1) is a circuit board or a plastic board.
10. The pushpin-like electrode for electroencephalogram recording of a young mouse according to claim 9, wherein said contact portion (3) is provided with an adhesive layer.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921548612.9U CN211094095U (en) | 2019-09-18 | 2019-09-18 | A pushpin appearance electrode for recording of young mouse electroencephalogram |
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| CN201921548612.9U CN211094095U (en) | 2019-09-18 | 2019-09-18 | A pushpin appearance electrode for recording of young mouse electroencephalogram |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110537912A (en) * | 2019-09-18 | 2019-12-06 | 浙江中医药大学 | A pushpin appearance electrode for recording of young mouse electroencephalogram |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110537912A (en) * | 2019-09-18 | 2019-12-06 | 浙江中医药大学 | A pushpin appearance electrode for recording of young mouse electroencephalogram |
| CN110537912B (en) * | 2019-09-18 | 2024-07-02 | 浙江中医药大学 | Drawing pin-like electrode for electroencephalogram recording of young mice |
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