CN211534401U

CN211534401U - Flexible recording electrode

Info

Publication number: CN211534401U
Application number: CN201922218495.6U
Authority: CN
Inventors: 王璐璐; 鲁艺; 钟成; 曹燚; 孙重阳; 王立平
Original assignee: Shenzhen Institute of Advanced Technology of CAS
Current assignee: Shenzhen Institute of Advanced Technology of CAS
Priority date: 2019-12-11
Filing date: 2019-12-11
Publication date: 2020-09-22
Anticipated expiration: 2029-12-11

Abstract

The utility model provides a flexible recording electrode, including adapter, many row's needles and many electrode wires, many row's needle intervals are located on the adapter, the one end of electrode wire with row's needle one-to-one is connected, many electrode wires are kept away from the one end of row's needle is passed through the tissue adhesive and is glued, the electrode wire is not the cladding the position cladding that the tissue was glued has the protection to glue. The flexible recording electrode of the utility model has small volume, can record the electrical activity of a target brain area with small area, and can lighten the load of the head of an animal; the flexibility is good, the wound electrode wire is fixed through tissue glue, the tissue glue is degradable in brain tissue, the electrode wire recovers the flexibility degree and can move along with the movement of the brain tissue, and the damage to the brain tissue is reduced; simple structure and low cost.

Description

Flexible recording electrode

Technical Field

The utility model belongs to the technical field of the recording electrode, especially, relate to a flexible recording electrode.

Background

In the nervous system, a large number of neurons continuously generate and transmit electrophysiological signals to realize information exchange between different brain regions and the neurons. Electrophysiological techniques have become a very important tool for our study of neuronal activity and hence neural circuit function due to their high temporal and spatial resolution recording advantage. In the past decades, the development of recording electrodes has made a series of significant breakthroughs, and these achievements have directly made a profound impact on neuroscience research, and rapidly driven the development of various novel electrode arrays including four electrodes capable of better distinguishing neuron types, michigan microelectrodes distributed at recording sites of different depths, uta microelectrode arrays for recording sites in a large range, and the like.

The method is a common method in the field of neuroscience, namely, the method is used for recording and researching the action potential of single cells in a brain area on a freely moving experimental animal, simultaneously testing the related behaviors of the freely moving experimental animal, and analyzing the neural loop function related to the specific behavior by processing the obtained data signals. This is helpful for functional analysis of the neural circuit.

However, most of the conventional metal electrodes are hard, and the Young's modulus of the electrodes is greatly different from that of brain tissue, so that the electrodes are easy to mechanically damage the brain tissue after being implanted. After the electrode is implanted into the brain tissue of the animal, the electrode can repeatedly penetrate through the nerve tissue in the moving process of the animal, and continuous damage is caused to the nerve tissue.

SUMMERY OF THE UTILITY MODEL

In view of the above, it is desirable to provide a flexible recording electrode with less brain tissue loss, and a method for manufacturing and implanting the same.

The utility model provides a flexible recording electrode, includes adapter, many row's needles and many electrode wires, many row's needle intervals are located on the adapter, the one end of electrode wire with row's needle one-to-one is connected, many electrode wires are kept away from the one end of row's needle is passed through the tissue adhesive and is glued, the electrode wire is not the cladding the position cladding that the tissue was glued has the protection to glue.

In one embodiment, the protective glue is an epoxy glue.

In one embodiment, the outer side of the tissue glue is further coated with polyethylene glycol.

In one embodiment, the wire electrode has a diameter of 12 μm, 17 μm or 25 μm.

The utility model discloses a flexible recording electrode, it is small, not only can the electric activity in the little target brain district of recording area, can alleviate the heavy burden of animal head moreover. In addition, above-mentioned flexible recording electrode is flexible good, fixes winding wire electrode through tissue glue, and tissue glue is degradable in the brain tissue, and the wire electrode resumes its flexibility degree, can follow the removal of brain tissue and remove, reduces the damage to brain tissue. Meanwhile, the flexible recording electrode is simple in structure and low in cost. The utility model discloses a preparation method of flexible recording electrode, easy operation, low cost, it is high-efficient to save time, and is lower to experimenter's technical requirement, can accord with the daily use in laboratory. The utility model discloses an implantation method of flexible recording electrode, easy operation easily realizes, and stability is good.

Drawings

FIG. 1 is a schematic view of a flexible recording electrode according to an embodiment;

FIG. 2 is a schematic structural view of a stent;

FIG. 3 is a schematic view of a trapezoidal structure;

FIG. 4 is a schematic winding of a wire electrode;

FIG. 5 is a flow chart of a method of manufacturing a flexible recording electrode according to one embodiment;

FIG. 6 is a flow chart of a method for implanting a flexible recording electrode according to one embodiment.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the drawings in the embodiments of the present invention are combined below to clearly and completely describe the technical solutions in the embodiments of the present invention. It is to be understood that the embodiments described are only some of the embodiments of the present invention, and not all of them. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

In the description of the present invention, it should be understood that the relation indicating the direction or position such as "up" is based on the direction or position shown in the drawings, or the direction or position conventionally placed when the utility model is used, or the direction or position conventionally understood by those skilled in the art, and is only for convenience of description and simplification of the description, and it does not indicate or imply that the indicated device or element must have a specific direction, be constructed and operated in a specific direction, and thus, should not be construed as limiting the present invention.

Furthermore, the terms "first," "second," and the like are used merely to distinguish one description from another, and are not to be construed as indicating or implying relative importance.

The following describes in detail embodiments of the present invention with reference to the accompanying drawings.

As shown in fig. 1, the flexible recording electrode of an embodiment includes an adapter 10, a plurality of pins 20, and a plurality of wires 30. The adapter 10 is located at many row's of needle intervals, and the one end of wire electrode 30 and row's of needle 20 one-to-one are connected, and the one end that many wire electrodes 30 kept away from row's of needle 20 passes through the tissue glue and bonds, and the position cladding that wire electrode 30 did not coat tissue glue 40 has protection glue 50.

The flexible recording electrode has small volume, can record the electrical activity of a target brain area with small area, and can reduce the load of the head of an animal. In addition, the flexible recording electrode is good in flexibility, the wound electrode wire 30 is fixed through the tissue glue 40, the tissue glue 40 can be degraded in brain tissue, the electrode wire 30 recovers the flexibility degree, and can move along with the movement of the brain tissue, and damage to the brain tissue is reduced. Meanwhile, the flexible recording electrode is simple in structure and low in cost. The tissue gel 40 is not particularly limited, and may be suitably implanted and degraded.

In one embodiment, the protective glue 50 is an epoxy glue.

In one embodiment, the outer side of the tissue gel 40 is further coated with polyethylene glycol. Specifically, the polyethylene glycol may be polyethylene glycol having a molecular weight of 4000. When implanted into the brain of an experimental animal, the wire electrode 30 fixed by the tissue glue 40 has enough hardness to be implanted into the brain tissue if it is in the superficial brain region. If the brain area is a deep brain area, in order to improve the accuracy, a layer of polyethylene glycol can be coated on the outer side of the tissue glue 40 to improve the hardness, then the tissue glue is implanted, after the target brain area is reached, the polyethylene glycol on the outer layer of the electrode is dissolved by using normal saline, and then the whole flexible recording electrode and the skull are packaged and fixed by using dental cement.

In one embodiment, the wire electrode 30 may have a diameter of 12 μm, 17 μm, or 25 μm. In order to record signals in a brain region with a small area, the size of the wire electrode 30 should be as small as possible, and the damage to the brain tissue is also smaller. Further, the wire electrode 30 may be a wire electrode 30.

When the diameter of the electrode wire is 12 μm, 8 channels of flexible recording electrodes are closely arranged, and the width of the electrode wire is less than 100 μm. The volume is small, and the device is particularly suitable for brain implantation.

The flexible recording electrode can be prepared into 8 channels or 16 channels according to experimental requirements.

The flexible recording electrode is more suitable for a target brain area with a shallow layer and a small area.

In addition, referring to fig. 3 to 5, a method for manufacturing the flexible recording electrode includes the following steps:

and S110, providing a support. The stand includes a base 150, a first post 110, a second post 120, a third post 130, and a fourth post 140. One end of each of the first column 110, the second column 120, the third column 130 and the fourth column 140 is fixedly connected to the base 150. The first column 110, the second column 120, the third column 130 and the fourth column 140 are distributed on the base 150 in a trapezoidal shape, the first column 110 and the fourth column 140 are respectively located at two ends of the bottom side of the trapezoid, and the second column 120 and the third column 130 are respectively located at two ends of the top side of the trapezoid.

Specifically, referring to fig. 3, the AD side of the trapezoid is the top side of the trapezoid, and the BC side is the bottom side of the trapezoid.

In one embodiment, the support is printed by a 3D printer or welded by a metal material. Preferably, the support adopts 3D printing, and is quick, with low costs.

In one embodiment, the material of the first, second, third and

fourth columns

110, 120, 130, 140 may be metal, wood or other hard material.

S120, fixing the first end of the wire electrode 30 on the first pillar 110.

The wire electrode 30 may have a diameter of 12 μm, 17 μm or 25 μm.

S130, the wire electrode 30 sequentially bypasses the second column 120, the third column 130, and one side of the fourth column 140, bends around the fourth column 140, and then sequentially bypasses the third column 130, the second column 120, and the first column 110, wherein the second column 120 and the third column 130 are located on the same side of the wire electrode 30.

S140, after the wire electrode 30 is bent around the first column 110, repeating S130 until the number of the wire electrodes 30 between the second column 120 and the third column 130 reaches a preset value, stopping winding, and fixing the second end of the wire electrode 30 to the first column 110.

It is understood that when an 8-channel flexible recording electrode is prepared, when the number of the wire electrodes 30 between the second column 120 and the third column 130 reaches 8, the number of the wire electrodes 30 of the 8-channel flexible recording electrode is satisfied. Subsequently, the wire electrode 30 does not need to be wound any more. The preset value in S140 is set according to actual needs.

And S150, bonding the electrode wire 30 between the second column 120 and the third column 130 by tissue glue. It is understood that the tissue glue is not particularly limited, and meets the requirements of being suitable for implantation and degradability.

And S160, cutting the electrode wires 30 on the first column 110 and the fourth column 140, and cutting the electrode wires 30 bonded by the tissue glue between the second column 120 and the third column 130 to obtain the electrode wire assembly.

It is understood that the tissue glue is sufficiently dried when the cutting of the wire electrode 30 bonded with the tissue glue between the second and

third posts

120 and 130 is performed.

It is understood that when the wire electrode 30 bonded with the tissue glue between the second and

third posts

120 and 130 is cut, the wire electrode 30 may be cut from the middle position between the second and

third posts

120 and 130.

And S170, sequentially fixing the wire electrode 30 at one end without the tissue glue of the wire electrode 30 assembly to different pin headers 20.

And S180, coating the part of the wire electrode 30 which is not coated with the tissue glue by using a protective glue to obtain the flexible recording electrode.

The portion of the wire electrode 30 not covered by the tissue glue is covered by the protective glue, so that the wire electrode 30 can be protected from being damaged. One end of the wire electrode 30 bonded with tissue glue is used for implantation in brain tissue.

The preparation method of the flexible recording electrode is simple to operate, low in cost, time-saving, efficient, low in technical requirement on experimenters and capable of meeting daily use of laboratories.

In one embodiment, the outer side of the tissue glue is coated with polyethylene glycol.

In one embodiment, after each winding of the wire electrode 30, the wire electrode 30 is aligned such that the wire electrodes 30 on the second and

third posts

120, 130 are maintained in a parallel and close arrangement.

Referring to fig. 6, a method for implanting the flexible recording electrode includes the following steps:

and S210, calculating the depth of the target brain area.

S220, performing a brain region positioning operation.

And S230, implanting the flexible recording electrode into the target brain area according to the positioned brain area.

It will be appreciated that the flexible recording electrode may be a two-electrode or a four-electrode. And the wire electrode 30 of the flexible recording electrode may be a wire electrode of different sizes. After the preparation of the flexible recording electrode is finished, the flexible recording electrode needs to be modified and detected so as to ensure that the flexible recording electrode can normally record electrophysiological signals. Furthermore, the flexible recording electrode needs to be capable of recording spike of a single neuron after being modified and tested.

S240, fixing the flexible electrode and packaging the flexible electrode to the skull to complete implantation.

In S240, the flexible recording electrode is fixed to the skull bone using dental cement or other methods.

The implantation method of the flexible recording electrode is simple to operate, easy to implement and good in stability.

The utility model discloses a flexible recording electrode allows the daily use in laboratory on a large scale to solve and stabilize the problem of neuron's electric activity record in the target brain district to the free activity animal under specific behavioural paradigm, especially the little brain district of area, with the brain district function of relevant under the analytic this behavioural. In the in-service use process, the utility model discloses flexible recording electrode is not limited to the record of neuron signal, also can combine optic fibre to form photoelectrode and implant target brain district and carry out the light stimulation.

The above is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of improvements and decorations can be made without departing from the principle of the present invention, and these improvements and decorations should also be regarded as the protection scope of the present invention.

Claims

1. The utility model provides a flexible recording electrode, its characterized in that includes adapter, many row's needles and many wire electrodes, many row's needle intervals are located on the adapter, the one end of wire electrode with row's needle one-to-one is connected, many wire electrodes are kept away from the one end of row's needle is passed through the tissue adhesive and is glued, the wire electrode is not the cladding the position cladding that the tissue glued has the protection to glue.

2. The flexible recording electrode of claim 1, wherein the protective adhesive is an epoxy adhesive.

3. The flexible recording electrode of claim 1, wherein the outer side of said tissue glue is further coated with polyethylene glycol.

4. The flexible recording electrode of claim 1, wherein the wire electrode has a diameter of 12 μm, 17 μm, or 25 μm.