CN211079161U - Separation and purification device - Google Patents
Separation and purification device Download PDFInfo
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- CN211079161U CN211079161U CN201921658128.1U CN201921658128U CN211079161U CN 211079161 U CN211079161 U CN 211079161U CN 201921658128 U CN201921658128 U CN 201921658128U CN 211079161 U CN211079161 U CN 211079161U
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- bottle body
- plate
- side plate
- filtering membrane
- sliding
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Abstract
The utility model provides a separation and purification device, which comprises a filtering device, wherein the filtering device comprises a bottle body, a coarse filtering membrane is arranged at the upper part in the bottle body, a fine filtering membrane is arranged at the lower part in the bottle body, a liquid inlet is arranged at the upper end of the bottle body, a liquid outlet is arranged at the lower end of the bottle body, and a switch valve is arranged on the liquid outlet; the bottle body is arranged in the shell, a sealing cavity is arranged at the upper part of the shell, the bottle body penetrates through the sealing cavity, and the middle part of the bottle body, which is positioned between the coarse filtering membrane and the fine filtering membrane, is positioned in the sealing cavity; and a sliding rail and a sliding plate which are in sliding fit are arranged at the position right below the switch valve on the shell, and accommodating grooves are distributed on the sliding plate. The sealed cavity ensures that the temperature of the part between the coarse filtering membrane and the fine filtering membrane of the bottle body is stable, thereby ensuring the activity of the virus in the bottle body; slide and slide rail that slide below the bottle set up to set up a plurality of centrifuging tube on the slide, thereby can get liquid fast, it is convenient fast to collect plasmid virus liquid.
Description
Technical Field
The utility model relates to a biological plasmid and virus separation and purification technical field, concretely relates to separation and purification device.
Background
In the process of separating and purifying biological plasmid and virus, the activity of the virus needs to be ensured, the plasmid virus liquid is put into a filtering device, coarse filtration is firstly carried out to remove cell fragments, fine filtration is then carried out, the plasmid virus is filtered and collected, and the plasmid virus liquid is subpackaged through a centrifugal tube. The practical way is that the filter device is fixed, and then the plasmid virus liquid is received below the filter device through the centrifugal tube every time, so that the operation is troublesome, and the activity of the virus in the filter device is not well ensured.
Disclosure of Invention
To the problem among the prior art, the utility model provides a separation and purification device.
A separation and purification device, comprising a filtering device, characterized in that: the filtering device comprises a bottle body, a coarse filtering membrane is arranged at the upper part in the bottle body, a fine filtering membrane is arranged at the lower part in the bottle body, a liquid inlet is formed in the upper end of the bottle body, a liquid outlet is formed in the lower end of the bottle body, and a switch valve is arranged on the liquid outlet; a negative pressure port is arranged on the bottle body between the coarse filtering membrane and the fine filtering membrane, is close to the coarse filtering membrane and is externally connected with a pumping pump;
the bottle body is arranged in the shell, a sealing cavity is arranged at the upper part of the shell, the bottle body penetrates through the sealing cavity, and the middle part of the bottle body, which is positioned between the coarse filtering membrane and the fine filtering membrane, is positioned in the sealing cavity;
lie in on the casing be equipped with straight line shape slide rail under the ooff valve sliding fit has rather than parallel arrangement's rectangular shape slide on the slide rail be provided with a plurality of centrifuging tube storage tank along its long direction on the slide.
The utility model discloses a theory of operation: the sealed cavity ensures that the temperature of the part between the coarse filtration membrane and the fine filtration membrane of the bottle body is stable, centrifugal tubes are placed in the accommodating groove on the sliding plate, and after the single centrifugal tube is aligned to the switch valve and filled with the centrifugal tubes, other centrifugal tubes on the sliding plate are filled in sequence; then, the slide plate is taken down from the slide rail and replaced by another slide plate which is provided with an empty centrifuge tube.
Further comprises the following steps: the shell comprises a bottom plate, and a rear side plate, a left side plate and a right side plate which are fixedly connected with the bottom plate, wherein the upper parts of the rear side plate, the left side plate and the right side plate are fixedly connected with an upper plate, a lower plate and a front side plate, and a sealed cavity is formed among the rear side plate, the left side plate, the right side plate, the upper plate, the lower plate and the front side plate; the structure is simple and easy to assemble and mold.
Further comprises the following steps: the sections of the sliding rail and the sliding plate are rectangular, a rectangular sliding groove is formed in the position, corresponding to the sliding rail, of the sliding plate, and the sliding groove penetrates through the sliding plate; the long direction of the slide rail is arranged along the front-back direction of the shell.
Further comprises the following steps: and a positioning straight line is arranged on the upper surface of the bottom plate and is positioned under the switch valve, the positioning straight line is in contact with the sliding plate and is vertically arranged, and vertical identification straight lines are arranged on the right side surface of the sliding plate corresponding to the accommodating grooves. When the sliding plate is moved and the identification straight line is aligned with the positioning straight line, the containing groove corresponding to the identification straight line is positioned under the switch valve, and the liquid in the bottle body of the switch valve is opened and then just falls into the centrifugal tube in the containing groove.
The utility model has the advantages that: the sealed cavity ensures that the temperature of the part between the coarse filtering membrane and the fine filtering membrane of the bottle body is stable, thereby ensuring the activity of the virus in the bottle body; the slide plate and the slide rail are arranged below the bottle body in a sliding manner, and the slide plate is provided with a plurality of centrifuge tubes, so that liquid can be quickly taken, and plasmid virus liquid can be quickly and conveniently collected; the positioning straight line and the marking straight line are respectively arranged on the bottom plate and the sliding plate, so that the centrifugal tube is convenient to align to a switch valve for discharging liquid on the bottle body; the coarse filtering membrane and the fine filtering membrane are matched to improve the filtering effect; the utility model has the advantages of simple overall structure, convenient operation is swift, improves work efficiency.
Drawings
Fig. 1 is a schematic structural view of the present invention;
fig. 2 is a schematic cross-sectional structure diagram of the present invention.
In the figure, 1, a housing; 11. a slide rail; 2. a bottle body; 21. a sealing cover; 22. an on-off valve; 23. a coarse filtration membrane; 24. a fine filtration membrane; 25. a negative pressure port; 26. positioning a straight line; 3. sealing the cavity; 4. a slide plate; 41. marking a straight line; 42. a containing groove; 5. and (6) centrifuging the tube.
Detailed Description
The present invention will be described in detail with reference to the accompanying drawings. It should be noted that the terms of orientation such as left, middle, right, up and down in the examples of the present invention are only relative to each other or are referred to the normal use status of the product, and should not be considered as limiting.
A separation and purification device, as shown in fig. 1 and 2, comprising a filtering device, wherein the filtering device comprises a bottle body 2, a coarse filtering membrane 23 is arranged at the upper part in the bottle body 2, a fine filtering membrane 24 is arranged at the lower part in the bottle body 2, a liquid inlet is arranged at the upper end of the bottle body 2, a liquid outlet is arranged at the lower end of the bottle body, and a switch valve 22 is arranged on the liquid outlet; the bottle body 2 is cylindrical, an opening at the upper end of the bottle body 2 is a liquid inlet, a sealing cover 21 is covered on the bottle body 2 at the liquid inlet, an air inlet is arranged in the middle of the sealing cover 21, a funnel body is arranged at the lower part of the bottle body 2, a liquid outlet is arranged at an opening at the lower part of the funnel body, and the switch valve 22 is a manual switch valve; a negative pressure port 25 is arranged on the bottle body 2 between the coarse filtering membrane 23 and the fine filtering membrane 24, and the negative pressure port 25 is close to the coarse filtering membrane 23 and externally connected with a filtering pump; the bottle body 2 is arranged in the shell 1, the upper part of the shell 1 is provided with a sealing cavity 3, the bottle body 2 penetrates through the sealing cavity 3, and the middle part of the bottle body 2 between the coarse filtering membrane 23 and the fine filtering membrane 24 is positioned in the sealing cavity 3; a linear slide rail 11 is arranged right below the switch valve 22 on the shell 2, a long strip-shaped slide plate 4 arranged in parallel with the slide rail 11 is matched on the slide rail 11 in a sliding manner, and four centrifuge tube accommodating grooves 42 are arranged on the slide plate 4 along the length direction of the slide plate; the bottle body can be made of plastic or glass, the shell and the sliding rail can be made of plastic or metal, and the sliding plate can be made of wood, plastic or rubber; in addition, the filter fineness of coarse filtration membrane is 100um, and the filter fineness of fine filtration membrane is 0.45um, and coarse filtration membrane and the cooperation of fine filtration membrane can improve the filter effect, and traditional operation is then for after the cell piece was got rid of in the centrifugation, then directly filters the plasmid virus liquid with 0.45 um's filter membrane.
The shell 1 comprises a bottom plate, and a rear side plate, a left side plate and a right side plate which are fixedly connected with the bottom plate, wherein the upper parts of the rear side plate, the left side plate and the right side plate are fixedly connected with an upper plate, a lower plate and a front side plate, and the sealed cavity 3 is formed among the rear side plate, the left side plate, the right side plate, the upper plate, the lower plate and the front side plate; the suction filtration pump is fixed on the bottom plate and is positioned at the rear part of the bottom plate, and a connecting pipe between the suction filtration pump and the negative pressure port 25 penetrates through the lower layer plate; wherein, the rear side board is arranged obliquely towards the front of the shell, the composition structure of the shell is of a conventional design, and the composition characteristics are not shown by reference numbers.
The cross sections of the sliding rail 11 and the sliding plate 4 are rectangular, a rectangular sliding groove is formed in the position, corresponding to the sliding rail 11, of the sliding plate 4, the sliding groove penetrates through the sliding plate 4, and a handle is fixedly arranged at the outer end of the sliding plate 4.
A positioning straight line 26 is arranged on the upper surface of the bottom plate and is positioned right below the switch valve 22, the positioning straight line 26 is in contact with the sliding plate 4 and is vertically arranged, and vertical identification straight lines 41 are arranged on the right side surface of the sliding plate 4 corresponding to the accommodating groove 42.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the above embodiments, and that the foregoing embodiments and descriptions are provided only to illustrate the principles of the present invention without departing from the spirit and scope of the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (4)
1. A separation and purification device, comprising a filtering device, characterized in that: the filtering device comprises a bottle body, a coarse filtering membrane is arranged at the upper part in the bottle body, a fine filtering membrane is arranged at the lower part in the bottle body, a liquid inlet is formed in the upper end of the bottle body, a liquid outlet is formed in the lower end of the bottle body, and a switch valve is arranged on the liquid outlet; a negative pressure port is arranged on the bottle body between the coarse filtering membrane and the fine filtering membrane, is close to the coarse filtering membrane and is externally connected with a pumping pump;
the bottle body is arranged in the shell, a sealing cavity is arranged at the upper part of the shell, the bottle body penetrates through the sealing cavity, and the middle part of the bottle body, which is positioned between the coarse filtering membrane and the fine filtering membrane, is positioned in the sealing cavity;
lie in on the casing be equipped with straight line shape slide rail under the ooff valve sliding fit has rather than parallel arrangement's rectangular shape slide on the slide rail be provided with a plurality of centrifuging tube storage tank along its long direction on the slide.
2. The separation and purification device of claim 1, wherein: the shell comprises a bottom plate, and a rear side plate, a left side plate and a right side plate which are fixedly connected with the bottom plate, wherein an upper plate, a lower plate and a front side plate are fixedly connected to the upper parts of the rear side plate, the left side plate and the right side plate, and the sealed cavity is formed among the rear side plate, the left side plate, the right side plate, the upper plate, the lower plate and the front side plate.
3. A separation and purification apparatus according to claim 2, wherein: the sections of the sliding rail and the sliding plate are rectangular, a rectangular sliding groove is formed in the position, corresponding to the sliding rail, of the sliding plate, and the sliding groove penetrates through the sliding plate; the long direction of the slide rail is arranged along the front-back direction of the shell.
4. A separation and purification apparatus according to claim 3, wherein: and a positioning straight line is arranged on the upper surface of the bottom plate and is positioned under the switch valve, the positioning straight line is in contact with the sliding plate and is vertically arranged, and vertical identification straight lines are arranged on the right side surface of the sliding plate corresponding to the accommodating grooves.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921658128.1U CN211079161U (en) | 2019-09-30 | 2019-09-30 | Separation and purification device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921658128.1U CN211079161U (en) | 2019-09-30 | 2019-09-30 | Separation and purification device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211079161U true CN211079161U (en) | 2020-07-24 |
Family
ID=71641042
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921658128.1U Active CN211079161U (en) | 2019-09-30 | 2019-09-30 | Separation and purification device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211079161U (en) |
-
2019
- 2019-09-30 CN CN201921658128.1U patent/CN211079161U/en active Active
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