CN211057114U

CN211057114U - Fluorescent quantitative PCR instrument

Info

Publication number: CN211057114U
Application number: CN201921573158.2U
Authority: CN
Inventors: 王建荣; 徐红柳
Original assignee: Zhejiang Zhizao Instrument Co ltd
Current assignee: Zhejiang Zhizao Instrument Co ltd
Priority date: 2019-09-20
Filing date: 2019-09-20
Publication date: 2020-07-21
Anticipated expiration: 2029-09-20

Abstract

The utility model discloses a fluorescence quantitative PCR appearance, including fluorescence detection device, fluorescence detection device outside-in still includes the casing in proper order, a detection section of thick bamboo and lofting board, it has an enclosing cover to articulate on the casing, it has an inner cup to articulate on the detection section of thick bamboo, the outer fixed mounting of casing has a driving motor, a detection section of thick bamboo and casing swing joint, driving motor's output shaft drive detection section of thick bamboo is rotary motion around the axis of a detection section of thick bamboo, lofting board fixed connection is respectively on the both ends face of a detection section of thick bamboo, evenly set up the constant head tank of being no less than 2 on the lofting board, the interior week of constant head tank all is fixed with a rubber ring, the fixed sample liquid test tube of rubber ring. The utility model discloses the inaccurate scheduling problem of testing result that the temperature difference to current detection test tube caused improves. The utility model has the advantages of detect liquid be heated evenly, improved the accuracy and the precision of testing result.

Description

Fluorescent quantitative PCR instrument

Technical Field

The utility model relates to a biochemical equipment technical field especially involves a fluorescence quantitative PCR appearance.

Background

The development of the fluorescent quantitative PCR instrument is based on the technology of obtaining different fluorescent signals by different combination rates of double-stranded DNA and fluorescent substances and the air heating technology, and the instrument is clinically applied to realize early-stage rapid and accurate detection, can be used for a tracking means of chemotherapy cancer and the like, and achieves the purposes of early diagnosis and timely treatment of patients. Among the fluorescence testing process, the influence of temperature to the testing result of sample detection liquid is great, only set up one among the fluorescence detection device and put the appearance dish, the heater strip sets up in the bottom of putting the appearance dish, and the detection test tube of splendid attire fluorescence detection liquid directly places on the appearance dish, when the heater strip rapid heating intensifies like this usually, can rapid heating up with the bottom of the detection test tube of appearance dish direct contact, exceed the temperature range of its needs, can destroy the reaction of sample detection liquid, and the temperature of the detection liquid of different positions exists the difference in same test tube, influence the precision and the accuracy of testing result.

For this reason, improve the accuracy of testing result and be the utility model discloses the problem that will solve to guarantee that fluorescence quantitative PCR appearance detection efficiency is high, and can reduce the error of testing result.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to overcome prior art not enough, provide a fluorescence quantitative PCR appearance to solve the technical problem that the testing result that the temperature difference of the detection test tube among the prior art caused is inaccurate etc.

The utility model discloses a realize through following technical scheme: the utility model discloses a fluorescence quantitative PCR appearance, including fluorescence detection device, fluorescence detection device outside-in still includes casing, detection cylinder and lofting board in proper order, it has an enclosing cover to articulate on the casing, it has an inner cup to articulate on the detection cylinder, the outer fixed mounting of casing has a driving motor, the detection cylinder with casing swing joint, driving motor's output shaft drive the detection cylinder winds rotary motion is done to the axis of detection cylinder, lofting board fixed connection respectively is in on the both ends face of detection cylinder, evenly set up on the lofting board and be no less than 2 constant head tanks, the interior week of constant head tank all is fixed with a rubber ring, the rubber ring can unsettled fixed sample liquid test tube in the detection cylinder.

Preferably, in order to enable the heating wire to heat the exterior of the detection cylinder more uniformly, the exterior of the detection cylinder is wrapped by an asbestos mesh layer.

Preferably, in order to facilitate simultaneous detection of different detection liquid concentrations and facilitate marking and distinguishing, a partition plate is further arranged between the two positioning grooves.

Preferably, in order to improve the utility model discloses a detection efficiency can place detection liquid in batches, the quantity of putting the model is a plurality of, and a plurality of put the model and be in detect the footpath parallel arrangement of a section of thick bamboo.

Preferably, for the energy saving, improve the utility model discloses a thermal efficiency, the inside of casing has still wrapped up the insulating layer that the one deck glass fiber material was made.

The utility model discloses a fluorescence quantitative PCR appearance compares with prior art:

the utility model adopts the locating slot to realize the single placement of the test tube, so that the test tube can be more stably placed without falling;

the utility model discloses a be provided with a rubber ring in the constant head tank, the rubber ring can be firm fixes a position the detection test tube, makes the detection test tube can hang in the air and fix on the constant head tank, compares with the detection test tube direct and lofting plate direct contact among the prior art, the utility model discloses can avoid the bottom of the detection test tube with lofting plate direct contact can rapid heating up destroy the reaction of sample detection liquid, improve the accuracy of testing result;

the utility model adopts the technical scheme that the lofting disc fixedly arranged in the prior art is replaced by a rotatable detection cylinder, so that the difference of the temperature of detection liquid at different positions in the same test tube is eliminated in the detection process, and the precision of the detection result is further improved;

the utility model has the advantages of detect liquid be heated evenly, improved the accuracy and the precision of testing result.

Drawings

Fig. 1 is a schematic cross-sectional view of the present invention;

FIG. 2 is a perspective view of the present invention;

1. a housing; 11. an outer cover; 12. a thermal insulation layer; 13. a terminal bearing; 14. a rotating shaft; 2. a detection cylinder; 21. an inner cover; 22. placing a template; 23. positioning a groove; 24. a rubber ring; 25. a partition plate; 26. an asbestos web layer; 3. a drive motor; 4. a heating wire.

Detailed Description

The embodiments of the present invention will be described in detail below, and the present embodiment is implemented on the premise of the technical solution of the present invention, and a detailed implementation manner and a specific operation process are given, but the scope of the present invention is not limited to the following embodiments.

Example 1

Embodiment 1 discloses a fluorescence quantitative PCR instrument, the utility model discloses a fluorescence quantitative PCR instrument, which comprises a fluorescence detection device, wherein a heating wire 4 is arranged in the fluorescence detection device to heat a detection test tube, thereby ensuring the smooth completion of the PCR process; the fluorescence detection device sequentially comprises a shell 1, a detection cylinder 2 for placing a detection test tube and a sample placing plate 22 from outside to inside, wherein the shell 1 is hinged with an outer cover 11, and the outer cover 11 is used for sealing the whole fluorescence detection device and is fixed with the shell 1 through static friction;

as the preferable scheme, in order to save energy and improve the thermal efficiency of the utility model, the inside of the shell 1 is wrapped with the thermal insulation layer 12 made of a layer of glass fiber material.

The detection cylinder 2 is hinged with an inner cover 21, and the inner cover 21 is used for sealing the detection cylinder 2 and is fixed with the detection cylinder 2 through a bolt; the center of the outer wall of two end faces of the detection cylinder 2 is welded with a rotating shaft 14, the rotating shaft 14 is movably connected on the shell 1 through a terminal bearing 13, the terminal bearing 13 is fixedly installed on the shell 1, a driving motor 3 is fixedly arranged outside the shell 1 through threads, the model of the driving motor 3 is 60ktyz (tin-free Jiebang motor), an output shaft of the driving motor 3 is fixedly welded with the rotating shaft 14 at one end, and the output shaft of the driving motor 3 drives the detection cylinder 2 to do slow rotary motion around the axis of the detection cylinder 2, and 10 revolutions per minute; the detection cylinder 2 which can do rotary motion eliminates the difference of the temperature of the detection liquid at different parts in the same test tube in the detection process, thereby further improving the precision of the detection result;

as the above preferred solution, in order to make the heating wire 4 heat the outside of the detecting cylinder 2 more uniformly, the outside of the detecting cylinder 2 and the outside of the inner lid 21 are both wrapped with a asbestos mesh layer 26, because asbestos is not a combustible substance, and the iron wire can disperse the heat of the heating wire 4 contacting with the asbestos mesh layer into the detecting cavity, so that the outside of the detecting cylinder 2 is heated uniformly.

Put the model 22 and place on the central axis of detecting a section of thick bamboo 2 and its both ends respectively fixed connection on the both ends face of detecting a section of thick bamboo 2, put evenly seted up 7 constant head tanks 23 on the model 22, the interior week of constant head tank 23 all is fixed with a rubber ring 24, and rubber ring 24 can be in the fixed test tube of detecting of suspension in detecting a section of thick bamboo 2. Only one detection test tube is placed in each positioning groove 23, and the positioning grooves 23 can be used for placing the detection test tubes singly, so that the detection test tubes are placed more stably and cannot topple over; the rubber ring 24 can firmly position the detection test tube, and the rubber ring 24 can be heated to expand, so that the detection test tube can be suspended and fixed on the positioning groove 23 when the detection cylinder 2 rotates, and the condition that the detection test tube does not fall off is ensured, the reaction that the bottom of the detection test tube directly contacted with the lofting plate is quickly heated to destroy the sample detection liquid can be avoided, and the accuracy of the detection result is improved;

as the preferable scheme, in order to facilitate the simultaneous detection of different detection liquids and the marking and distinguishing, a partition plate 25 is further arranged between the two positioning grooves 23;

preferably, in order to improve the utility model discloses a detection efficiency, can place in batches and detect liquid, the quantity of putting the model 22 is a plurality of, and a plurality of putting the model 22 and radially parallel arrangement at detection section of thick bamboo 2.

The during operation is sealed in adding the test tube with the sample liquid of waiting to detect, then open enclosing cover 11 and inner cup 21, hang the test tube and fix in rubber ring 24 with detecting, if the concentration or the kind of the sample liquid of waiting to detect are different, then need place respectively on the constant head tank 23 that different baffles 25 separated, close inner cup 21 and enclosing cover 11 in proper order after placing the completion, energize heater strip 4 again, heat up according to the temperature requirement of the sample liquid of waiting to detect, switch on driving motor 3's power makes it rotate slowly in detecting the intracavity, the thermally equivalent.

Claims

1. The utility model provides a fluorescence quantitative PCR appearance, includes fluorescence detection device, its characterized in that, fluorescence detection device outside-in still includes casing, detection cylinder and lofting board in proper order, it has an enclosing cover to articulate on the casing, it has an inner cup to articulate on the detection cylinder, the outer fixed mounting of casing has a driving motor, the detection cylinder with casing swing joint, driving motor's output shaft drive the detection cylinder winds rotary motion is to the axis of detection cylinder, lofting board fixed connection respectively is in on the both ends face of detection cylinder, evenly set up on the lofting board and be no less than 2 constant head tanks, the inner periphery of constant head tank all is fixed with a rubber ring, the rubber ring can unsettled fixed sample liquid test tube in the detection cylinder.

2. The fluorescence quantitative PCR instrument of claim 1, wherein the detection cylinder is externally wrapped with an asbestos mesh layer.

3. The fluorescence quantitative PCR instrument of claim 1, wherein a partition is further disposed between the two positioning grooves.

4. The fluorescence quantitative PCR instrument as claimed in claim 1, wherein the number of the sample placing plates is plural, and the plural sample placing plates are arranged in parallel in a radial direction of the detection cylinder.

5. The fluorescence quantitative PCR instrument of claim 1, wherein the inside of the housing further comprises a heat insulating layer made of glass fiber material.