CN210993113U

CN210993113U - Protein purification chromatographic column

Info

Publication number: CN210993113U
Application number: CN201921800239.1U
Authority: CN
Inventors: 曾建华; 闫云云; 胡雪平
Original assignee: Wuhan Huatai Biotechnology Co ltd
Current assignee: Wuhan Huatai Biotechnology Co ltd
Priority date: 2019-10-24
Filing date: 2019-10-24
Publication date: 2020-07-14
Anticipated expiration: 2029-10-24

Abstract

The utility model relates to a protein purification technical field just discloses a protein purification chromatography post, the on-line screen storage device comprises a base, the bottom fixedly connected with ground connection post of base, the left side fixedly connected with branch at base top, the sliding sleeve has been cup jointed to the surface of branch, the left side fixedly connected with of sliding sleeve runs through the sliding sleeve and extends to the inside screw thread adjust knob of branch, the right side fixedly connected with anchor clamps of sliding sleeve. This protein purification chromatographic column is connected with the peristaltic pump through the one side that sets up the raceway, the velocity of flow of control liquid that can be accurate does not need the manual operation extrusion, then has three receiving flask through setting up fixed disk top joint, one side fixedly connected with of peristaltic pump extends to the connecting pipe at receiving flask top, conveniently collects the protein after the purification, has reached the efficient effect of purification to the effectual problem of the control velocity of flow that can not be accurate in the protein purification process influences protein purification efficiency.

Description

Protein purification chromatographic column

Technical Field

The utility model relates to a protein purification technical field specifically is a protein purification chromatographic column.

Background

The isolation and purification of proteins is a widely used technique in biochemical research applications, and a typical eukaryotic cell may contain thousands of different proteins, some very abundant, some only in a few copies, and in order to study a certain protein, it is necessary to first purify the protein from other proteins and non-protein molecules.

Along with the rapid development of science and technology, the application of protein has been very extensive, and the purification of protein needs to use the chromatographic column, and the chromatographic column on the market at present mostly uses manual pressure that provides, makes the liquid flow direction collecting flask, and this kind of mode flexible operation, but the operation is very inconvenient when equivalent is many, influences production efficiency, so provides a protein purification chromatographic column and solves the problem that provides in the aforesaid.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

The utility model provides a not enough to prior art, the utility model provides a protein purification chromatographic column possesses advantages such as purification efficiency height, has solved the problem that the control flow rate that can not be accurate influences protein purification efficiency among the protein purification process.

(II) technical scheme

For realizing the purpose of the above-mentioned purification efficiency, the utility model provides a following technical scheme: a protein purification chromatographic column comprises a base, wherein a grounding column is fixedly connected to the bottom of the base, a supporting rod is fixedly connected to the left side of the top of the base, a sliding sleeve is sleeved on the outer surface of the supporting rod, a thread adjusting knob penetrating through the sliding sleeve and extending into the supporting rod is fixedly connected to the left side of the sliding sleeve, a clamp is fixedly connected to the right side of the sliding sleeve, a cylinder body is fixedly connected to the inside of the clamp, a cover cap is fixedly connected to the top of the cylinder body, a column head is connected to the bottom of the cylinder body through threads, a control valve penetrating through and extending to the outside of the column head is fixedly connected to the outer surface of the column head, a first hydrophilic sieve plate is fixedly connected to the inside of the cylinder body, a second hydrophilic sieve plate is fixedly connected to the bottom of the first hydrophilic sieve plate, and a third hydrophilic sieve plate, the bottom fixedly connected with raceway of column cap, the junction fixedly connected with sealing washer of raceway and column cap, the one end fixedly connected with peristaltic pump that the column cap was kept away from to the raceway, the right side fixedly connected with fixed disk of base, the top joint of fixed disk has the receiving flask, the one end fixedly connected with that the raceway was kept away from to the peristaltic pump extends to the connecting pipe at receiving flask top.

Preferably, the number of the grounding posts is four, the grounding posts are uniformly distributed at four corners of the bottom of the base in a rectangular shape, and the bottom of each grounding post is fixedly connected with a rubber anti-slip pad.

Preferably, branch is solid cylinder, the thread groove has been seted up on the left side of branch, the internal diameter size of sliding sleeve equals the external diameter size of branch.

Preferably, the connection mode of anchor clamps and bracing piece is the welding, the round hole of cylinder external diameter size is seted up to the inside of anchor clamps, the junction fixedly connected with rubber pad of anchor clamps and cylinder, the cylinder is hollow cylinder, the surface of cylinder is equipped with the capacity mark.

Preferably, the column head is in threaded connection with the column body, the bottom of the column head is provided with a guide outlet, and the water delivery pipe is a soft hose.

Preferably, the draw-in groove has been seted up at the top of fixed disk, the diameter size of draw-in groove equals the external diameter size of receiving flask, the receiving flask is hollow cylinder, the quantity of receiving flask is three, and the surface of receiving flask all is equipped with the capacity mark.

(III) advantageous effects

Compared with the prior art, the utility model provides a protein purification chromatographic column possesses following beneficial effect:

1. this protein purification chromatography column, through setting up the raceway that column cap bottom fixedly connected with extends to the peristaltic pump, the convenient transport protein, one side that the rethread set up the raceway is connected with the peristaltic pump, the velocity of flow of control liquid that can be accurate does not need the manual operation extrusion, then has three receiving flask through setting up fixed disk top joint, one side fixedly connected with of peristaltic pump extends to the connecting pipe at receiving flask top, conveniently collects the protein after the purification, has reached the efficient effect of purification.

2. This protein purification chromatography column, through four earthing columns that set up the base bottom, and the rubber slipmat of earthing column bottom, can effectively strengthen holistic stability, through setting up the inside first hydrophilicity sieve of cylinder, second hydrophilicity sieve and third hydrophilicity sieve, can effectively improve the quality of protein purification, through the junction fixedly connected with sealing washer that sets up raceway and column cap, can effectively avoid the condition that leaks to appear in the column cap, thereby the effectual problem of the control flow rate that can not be accurate influences protein purification efficiency in the protein purification process of having solved.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

In the figure: the device comprises a base 1, a grounding column 2, a support rod 3, a sliding sleeve 4, a thread adjusting knob 5, a clamp 6, a column 7, a cap 8, a column cap 9, a control valve 10, a first hydrophilic sieve plate 11, a second hydrophilic sieve plate 12, a third hydrophilic sieve plate 13, a water delivery pipe 14, a sealing ring 15, a peristaltic pump 16, a fixed disc 17, a collecting bottle 18 and a connecting pipe 19.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1, a protein purification chromatography column comprises a base 1, a grounding column 2 is fixedly connected to the bottom of the base 1, the number of the grounding column 2 is four, and the grounding column 2 is uniformly distributed at four corners of the bottom of the base 1 in a rectangular shape, a rubber anti-slip pad is fixedly connected to the bottom of the grounding column 2, a supporting rod 3 is fixedly connected to the left side of the top of the base 1, a sliding sleeve 4 is sleeved on the outer surface of the supporting rod 3, the supporting rod 3 is a solid cylinder, a threaded groove is formed in the left side of the supporting rod 3, the inner diameter of the sliding sleeve 4 is equal to the outer diameter of the supporting rod 3, a threaded adjusting knob 5 penetrating through the sliding sleeve 4 and extending into the supporting rod 3 is fixedly connected to the left side of the sliding sleeve 4, a clamp 6 is fixedly connected to the right side of the sliding sleeve 4, a cylinder 7 is fixedly connected to the inside of, a rubber pad is fixedly connected at the joint of the clamp 6 and the column 7, the column 7 is a hollow cylinder, a capacity mark is arranged on the outer surface of the column 7, a cap 8 is fixedly connected at the top of the column 7, a column cap 9 is connected at the bottom of the column 7 in a threaded manner, a control valve 10 which penetrates through and extends to the outside of the column cap 9 is fixedly connected on the outer surface of the column cap 9, a first hydrophilic sieve plate 11 is fixedly connected inside the column 7, a second hydrophilic sieve plate 12 is fixedly connected inside the column 7 and positioned at the bottom of the first hydrophilic sieve plate 11, a third hydrophilic sieve plate 13 is fixedly connected inside the column 7 and positioned at the bottom of the second hydrophilic sieve plate 12, a water delivery pipe 14 is fixedly connected at the bottom of the column cap 9, the column cap 9 is connected with the column 7 in a threaded manner, a guide outlet is formed at the bottom of the column cap 9, the water delivery pipe 14 is, the protein purification chromatographic column can effectively enhance the overall stability by arranging the four grounding columns 2 at the bottom of the base 1 and the rubber non-slip mats at the bottoms of the grounding columns 2, can effectively improve the quality of protein purification by arranging the first hydrophilic sieve plate 11, the second hydrophilic sieve plate 12 and the third hydrophilic sieve plate 13 inside the column body 7, can effectively avoid the situation of water leakage of the column head 9 by arranging the sealing ring 15 fixedly connected at the joint of the water delivery pipe 14 and the column head 9, the end of the water delivery pipe 14 far away from the column head 9 is fixedly connected with the peristaltic pump 16, the right side of the base 1 is fixedly connected with the fixed disk 17, the top of the fixed disk 17 is clamped with the collection bottle 18, the top of the fixed disk 17 is provided with a clamping groove, the diameter of the clamping groove is equal to the outer diameter of the collection bottle 18, the collection bottle 18 is a hollow cylinder, the number of the collection bottles 18 is three, and the outer surfaces, peristaltic pump 16 keeps away from one end fixedly connected with of raceway 14 and extends to the connecting pipe 19 at collection bottle 18 top, this protein purification chromatographic column, raceway 14 that extends to peristaltic pump 16 through setting up column cap 9 bottom fixedly connected with, make things convenient for the transport protein, one side that the rethread set up raceway 14 is connected with peristaltic pump 16, the velocity of flow of control liquid that can be accurate, do not need manual operation extrusion, then there is three collection bottle 18 through setting up fixed disk 17 top joint, one side fixedly connected with of peristaltic pump 16 extends to the connecting pipe 19 at collection bottle 18 top, conveniently collect the protein after the purification, the efficient effect of purification has been reached, thereby the effectual problem that can not accurate control the velocity of flow influence protein purification efficiency in the protein purification process of having solved.

To sum up, this protein purification chromatographic column, through setting up the raceway 14 that column cap 9 bottom fixedly connected with extends to peristaltic pump 16, conveniently transport protein, one side that the rethread set up raceway 14 is connected with peristaltic pump 16, the velocity of flow of control liquid that can be accurate does not need the manual operation extrusion, then there is three receiving flask 18 through setting up fixed disk 17 top joint, one side fixedly connected with of peristaltic pump 16 extends to the connecting pipe 19 at receiving flask 18 top, conveniently collect the protein after the purification, the efficient effect of purification has been reached.

And, this protein purification chromatographic column, through setting up four earthing columns 2 of base 1 bottom, and the rubber slipmat of earthing column 2 bottom, can effectively strengthen holistic stability, through setting up the inside first hydrophilicity sieve 11 of cylinder 7, second hydrophilicity sieve 12 and third hydrophilicity sieve 13, can effectively improve protein purification's quality, through setting up the junction fixedly connected with sealing washer 15 of raceway 14 and column cap 9, can effectively avoid column cap 9 to appear the condition of leaking, thereby the effectual problem of the control velocity of flow that can not be accurate influences protein purification efficiency in the protein purification process of having solved.

It is to be noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A protein purification chromatographic column, includes base (1), its characterized in that: the bottom fixedly connected with earthing pole (2) of base (1), the left side fixedly connected with branch (3) at base (1) top, sliding sleeve (4) has been cup jointed to the surface of branch (3), the left side fixedly connected with of sliding sleeve (4) runs through sliding sleeve (4) and extends to the inside screw thread adjust knob (5) of branch (3), the right side fixedly connected with anchor clamps (6) of sliding sleeve (4), the inside fixedly connected with cylinder (7) of anchor clamps (6), the top fixedly connected with of cylinder (7) is capped (8), the bottom threaded connection of cylinder (7) has column cap (9), fixedly connected with runs through and extends to the outside control valve (10) of column cap (9) on the surface of column cap (9), the inside fixedly connected with first hydrophilicity sieve board (11) of cylinder (7), the utility model discloses a solar water heater, including cylinder (7), the inside of cylinder (7) and bottom fixedly connected with second hydrophilicity sieve (12) that are located first hydrophilicity sieve (11), the inside of cylinder (7) and the bottom fixedly connected with third hydrophilicity sieve (13) that are located second hydrophilicity sieve (12), the bottom fixedly connected with raceway (14) of column cap (9), the junction fixedly connected with sealing washer (15) of raceway (14) and column cap (9), the one end fixedly connected with peristaltic pump (16) of column cap (9) are kept away from in raceway (14), the right side fixedly connected with fixed disk (17) of base (1), the top joint of fixed disk (17) has receiving flask (18), the one end fixedly connected with that raceway (14) were kept away from in peristaltic pump (16) extends to connecting pipe (19) at receiving flask (18) top.

2. The protein purification chromatography column of claim 1, wherein: the number of the grounding posts (2) is four, the four grounding posts are uniformly distributed at four corners of the bottom of the base (1) in a rectangular mode, and rubber anti-slip pads are fixedly connected to the bottom of the grounding posts (2).

3. The protein purification chromatography column of claim 1, wherein: the supporting rod (3) is a solid cylinder, a thread groove is formed in the left side of the supporting rod (3), and the inner diameter of the sliding sleeve (4) is equal to the outer diameter of the supporting rod (3).

4. The protein purification chromatography column of claim 1, wherein: the connecting mode of anchor clamps (6) and branch (3) is the welding, the round hole of cylinder (7) external diameter size is seted up to the inside of anchor clamps (6), the junction fixedly connected with rubber pad of anchor clamps (6) and cylinder (7), cylinder (7) are hollow cylinder, the surface of cylinder (7) is equipped with the capacity mark.

5. The protein purification chromatography column of claim 1, wherein: the column cap (9) is connected with the column body (7) in a threaded manner, a guide outlet is formed in the bottom of the column cap (9), and the water delivery pipe (14) is a flexible hose.

6. The protein purification chromatography column of claim 1, wherein: the top of fixed disk (17) has been seted up the draw-in groove, the diameter size of draw-in groove equals the external diameter size of receiving flask (18), receiving flask (18) are hollow cylinder, the quantity of receiving flask (18) is three, and the surface of receiving flask (18) all is equipped with the capacity mark.