CN210932006U

CN210932006U - NEC experimental facilities

Info

Publication number: CN210932006U
Application number: CN201921323910.8U
Authority: CN
Inventors: 胡刘宏; 刘斌
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-08-15
Filing date: 2019-08-15
Publication date: 2020-07-07
Anticipated expiration: 2029-08-15

Abstract

The utility model discloses a NEC experimental facility, which comprises a feeding box, an experimental box, a conveying pipe and a control device, wherein the conveying pipe is used for feeding small animals into the experimental box, one end of the conveying pipe is connected with the feeding box, the other end of the conveying pipe is connected with the experimental box, the conveying pipe is arranged in an inclined way, the feeding box is provided with a rotatable bottom plate, a rotating shaft for rotating the bottom plate is arranged on the bottom plate, one end of the bottom plate is provided with a driving device for driving the bottom plate to rotate so as to tilt one end of the bottom plate, the conveying pipe is provided with an induction door at one side of the experimental box, when the small animals need to be fed into the experimental box, the control device controls the driving device to jack up one end of the bottom plate so as to make the other end of the bottom plate move downwards, so that the bottom plate is inclined so that the small animals can slide into the conveying pipe, the, the whole process does not need manual operation, and the labor intensity of workers is greatly reduced.

Description

NEC experimental facilities

Technical Field

The utility model relates to a medical experimental apparatus technical field, in particular to NEC experimental facilities.

Background

Necrotizing enterocolitis of newborn (i.e., NEC) is a common and very serious gastrointestinal emergency in the neonatal period, the incidence of disease reaches 7% in premature infants with a birth weight of less than 1500g, the mortality rate can reach 20% -30%, and in addition, 25% of surviving infants can have serious sequelae such as intestinal fistula, short bowel syndrome, after-growth and impaired development of the nervous system. To date, much research has been done on the disease at home and abroad, but the pathogenesis is still unclear.

In order to explore the pathogenesis of the disease and further provide intervention measures, a stable NEC animal model is often required to be established, and the stable NEC animal model is established at home and abroad by adopting a mode of artificial feeding, hypoxia and cold stimulation of a newborn mouse. The artificial feeding is that a mouse milk substitute product prepared artificially is subjected to oral gavage treatment through a PICC (the PICC is a catheter inserted into an upper vena cava through a peripheral vein), one person can only feed 1 newborn mouse at a time, the smooth feeding of one newborn mouse takes about 1.5min (minute), about 30 newborn mice are fed every time, and the feeding needs to be repeated for 1 time every 4 hours, namely, people are required to be on duty at night and in the morning for feeding treatment, more importantly, the gavage of the newborn mouse is a very challenging technology, and the death of the newborn mouse can be caused by the asphyxia or the lung hemorrhage due to the fact that the grabbing and pinching force is too large, the PICC is inserted too deeply, too shallowly or inserted into an airway by mistake during each gastric gavage operation, so that the experiment cannot be continued. Meanwhile, the artificial feeding is designed in an experiment to simulate the high-risk factor that the clinical NEC infant is not breast-fed but is fed by formula milk, but the clinical operation that the infant is not repeatedly gazed is avoided, so that the mode of artificially gavage feeding the newborn mouse is time-consuming and labor-consuming, and the clinical simulation is not good.

In addition, when oxygen deficiency and cold stimulation are carried out, nitrogen is filled into a self-made simple oxygen deficiency box, when an oxygen instrument to be measured measures that the oxygen concentration in the box is reduced to 0%, a box cover is opened, all the newborn mice are placed into the oxygen deficiency box to be subjected to oxygen deficiency treatment, the newborn mice are taken out after 10min, and after oxygen is subjected to temporary reoxygenation, the newborn mice are placed into a refrigerator at 4 ℃ to be subjected to cold stimulation intervention for 10min, so that the NEC animal model is established. In the experimental mode, when the mouse needs to be placed in the anoxic treatment process, the cover is opened, and the absolute zero oxygen concentration in the box cannot be ensured. When the mouse is in cold stimulation, the mouse is directly placed in a refrigerator filled with various experimental reagents or experimental articles, unnecessary pollution is easily caused, and each step needs manual participation, so that the mouse is not intelligent enough and the labor cost is too high.

In summary, the prior experimental techniques need to be improved and improved.

Disclosure of Invention

In view of the above-mentioned prior art's weak point, the utility model aims to provide a NEC experimental facilities can automize and feed the toy, and make the toy automatically send into the experimental box.

In order to achieve the purpose, the utility model adopts the following technical proposal:

the utility model provides a NEC experimental facilities, is including feeding case, experimental box, being used for sending into the conveyer pipe and the controlling means of experimental box with the toy, the case is fed in the one end connection of conveyer pipe, and the experimental box is connected to the other end of conveyer pipe, the conveyer pipe slope sets up, it is provided with rotatable bottom plate to feed the case, be provided with on the bottom plate and supply bottom plate pivoted axis of rotation, the one end of bottom plate is provided with and is used for driving the bottom plate to rotate the drive arrangement who makes the one end perk of bottom plate, drive arrangement is connected with the controlling means electricity.

In the NEC experimental facility, be provided with the feeder in feeding the case, the feeder has the stock solution chamber that is used for storing formula milk, the bottom of feeder has a plurality of nipples that are used for supplying to be fed the animal and suck, and the upper portion of feeder is provided with annotates the liquid mouth, nipple and annotate the liquid mouth and all communicate with the stock solution chamber.

The NEC experimental facility, still include the negative pressure air exhaust device that is used for taking out the oxygen in the experimental box clean, be used for the oxygen conveyor of experimental box oxygen suppliment and be used for making the cryogenic nitrogen gas conveyor of experimental box, oxygen conveyor, negative pressure air exhaust device and nitrogen gas conveyor all are connected with controlling means electricity.

In the NEC experimental equipment, the experimental box is a double-layer box body, the first layer is an outer box, the second layer is an inner box, and a preset interval is arranged between the outer box and the inner box.

In the NEC experimental equipment, an oxygen input pipe, an oxygen output pipe and a nitrogen input pipe are connected to the experimental box, one ends of the oxygen input pipe and the oxygen output pipe penetrate through the outer box and are communicated with the inner box, the other end of the oxygen input pipe is connected with an oxygen conveying device, and the other end of the oxygen output pipe is connected with a negative pressure air extraction device; one end of the nitrogen input pipe is communicated with the outer box, and the other end of the nitrogen input pipe is connected with the nitrogen conveying device.

In the NEC experimental equipment, electromagnetic valves are arranged on the oxygen input pipe, the oxygen output pipe and the nitrogen input pipe and are connected with a control device.

In the NEC experimental facility, the feeding box, the feeder and the experimental box are all made of transparent materials.

In the NEC experimental equipment, a temperature sensor for detecting the temperature of the inner box is arranged in the inner box, and the temperature sensor is connected with a control device.

In the NEC experimental equipment, an oxygen meter for detecting the oxygen concentration of the inner box is arranged in the inner box and is connected with a control device.

In the NEC experimental equipment, a sealing door is arranged at the outlet of the conveying pipe of the experimental box.

In the NEC experimental equipment, a sucker device used for enabling the feeder to be stuck in the feeding box is arranged on the feeder.

Compared with the prior art, the utility model provides a NEC experimental facilities, including feeding case, experimental box, being used for sending into the conveyer pipe and the controlling means of experimental box with the toy, the case is fed in the one end connection of conveyer pipe, and the experimental box is connected to the other end of conveyer pipe, the conveyer pipe slope sets up, it is provided with rotatable bottom plate to feed the case, be provided with on the bottom plate and supply bottom plate pivoted axis of rotation, the one end of bottom plate is provided with and is used for driving the bottom plate to rotate the drive arrangement who makes the one end perk of bottom plate, drive arrangement is connected with the controlling means electricity. The toy can be fed in feeding the case, when needs sent the toy to the experimental box, controlling means control drive arrangement made the one end jack-up of bottom plate move down the other end of bottom plate, made the bottom plate slope let the toy can slide in the conveyer pipe, slide in the experimental box through the conveyer pipe, whole process does not need the manual work to operate, has alleviateed staff's intensity of labour greatly, the effectual work efficiency that has improved.

Drawings

Fig. 1 is a schematic structural diagram of the NEC experimental apparatus provided by the present invention.

Fig. 2 is a schematic diagram of the bottom plate of the feeding box of the NEC experimental facility provided by the present invention when it is inclined.

Detailed Description

In view of the deficiencies of the prior art, the utility model aims to provide a NEC experimental facilities can feed the toy voluntarily, realizes feeding and experiment integration.

In order to make the objects, technical solutions and effects of the present invention clearer and clearer, the following description of the present invention will refer to the accompanying drawings and illustrate embodiments. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the invention.

Please refer to fig. 1 and fig. 2, the utility model provides a NEC experimental apparatus, including feeding case 1, experimental box 2, be used for sending the toy into experimental box 2's conveyer pipe 3 and controlling means (not shown in the figure), feeding case 1 is connected to the one end of conveyer pipe 3, experimental box 2 is connected to the other end of conveyer pipe 3, specifically, the both ends of conveyer pipe 3 are all connected in feeding case 1 and experimental box 2's bottom to the tumble injury or death when the toy (like little mouse) gets into experimental box 2, the conveyer pipe 3 slope sets up, is located the one end of feeding case 1 and is higher than experimental box 2's one end, makes the toy can slide smoothly to the experimental box 2 in through conveyer pipe 3.

In this embodiment, the inner wall of conveyer pipe 3 is smooth, makes the toy directly slide into implement the case in, it is provided with rotatable bottom plate 4 to feed case 1, be provided with on bottom plate 4 and supply bottom plate 4 to rotate the axis of rotation 5 that makes the one end perk of bottom plate 4, the one end of bottom plate 4 is provided with and is used for driving bottom plate 4 to rotate the drive arrangement 6 that makes the one end perk of bottom plate 4, drive arrangement 6 is connected with the controlling means electricity.

When the small animal is required to be conveyed to the experimental box 2, the control device controls the driving device 6 to jack up one end of the bottom plate 4 to enable the other end of the bottom plate 4 to move downwards, so that the bottom plate 4 is inclined to enable the small animal to slide into the conveying pipe 3, the small animal enters the conveying pipe 3, then the inclined conveying pipe 3 slides into the experimental box 2, the whole process does not need manual operation, and the labor intensity of workers is greatly reduced.

With continuing reference to fig. 1 and 2, a feeder 11 is arranged in the feeding box 1, the feeder 11 has a liquid storage chamber 15 for storing formula milk, the bottom of the feeder 11 has a plurality of nipples 12 for being sucked by the fed animals, the upper part of the feeder 11 is provided with a liquid injection port 13, the liquid injection port 13 is provided with a sealing plug 14 for preventing liquid from overflowing, and the nipples 12 and the liquid injection port 13 are both communicated with the liquid storage chamber 15.

Preferably, the feeding device 11 is further provided with a temperature control device, which comprises a heating wire (not shown in the figure), a temperature measuring device (not shown in the figure) for detecting the temperature of the liquid (i.e. the milk temperature), a control chip and a battery, wherein the heating wire, the temperature measuring device and the battery are all connected with the control chip.

In this embodiment, the control chip may be an integrated chip of NRF51822_ QFAA, which is an ultra-low power consumption MCU and has the functions of fast data processing, multiple expansion interfaces, stable performance, sleep, intermittent wake-up, ultra-low power consumption, etc. The temperature measuring device can adopt a temperature sensor and the like, can be partially arranged at the bottom of the liquid storage cavity 15, the heating wire can be uniformly wound on the middle lower part of the feeder 11 and is respectively connected with an IO port (output port) of the control chip, and the heating temperature of the heating wire is controlled through detected temperature data, so that the milk temperature of the liquid storage cavity 15 is constant. The liquid can be heated to the preset temperature and kept in the preset temperature range through the heating wire and the temperature measuring device, and the small animal feels that the temperature can automatically approach the sucking nipple.

In addition, the feeder 11 can be designed into the appearance of a mother of a small animal (such as the appearance of a mouse mother), so that the small animal can be used for breast feeding and can independently suck and prepare milk, the feeding mode is feeding according to needs, gastric perfusion is not needed, clinic simulation can be better realized, and meanwhile, the death rate of the small animal is greatly reduced, so that the subsequent NEC experiment can be conveniently carried out.

The feeding mode is used for feeding according to the need, so that the small animals are healthier, the NEC experiment can be conveniently carried out, a plurality of small animals can be fed at one time, personnel participation is not needed, and the feeding efficiency is improved.

In order to facilitate the oxygen deficiency experiment, the NEC experimental apparatus further comprises a negative pressure air extractor (not shown in the figure) for extracting oxygen from the experiment box 2, an oxygen delivery device (not shown in the figure) for supplying oxygen to the experiment box 2, and a nitrogen delivery device (not shown in the figure) for refrigerating the experiment box 2, wherein the oxygen delivery device, the negative pressure air extractor and the nitrogen delivery device are all electrically connected with the control device.

In the experimental process, through negative pressure air exhaust device can take out the oxygen in experimental box 2 to the greatest extent fast, when waiting to oxygenate for experimental box 2 in, through oxygen conveyor can give experimental box 2 interior oxygen suppliment fast. In addition, an oxygen measuring instrument for detecting the oxygen concentration of the inner box can be arranged in the inner box and connected with the control device, and when the oxygen measuring instrument detects that the oxygen concentration of the inner box is 0, the control device controls the negative pressure air exhaust device to stop working. The utility model discloses of course also can directly set up negative pressure air exhaust device's operating time according to the volume of experimental box, make the oxygen in experimental box 2 take out to the greatest extent, this mode can calculate according to the volume of experimental box, also can directly use external oxygen concentration detector to detect the experiment, reachs the required time of evacuation.

When a cold stimulation experiment is carried out, the nitrogen conveying device is used for keeping the experiment box 2 in a low-temperature state (generally about 4 ℃), the time for filling the nitrogen of the nitrogen conveying device can be set according to the volume of the experiment box to control the temperature required by the cold stimulation experiment, the conveying of the nitrogen can be stopped when the temperature of the experiment box reaches the target temperature detected by the thermometer, and the time required by filling the nitrogen can be obtained through the experiment in the same way.

Referring to fig. 1 and 2, the experimental box 2 is a double-layer box, the first layer is an outer box 21, the second layer is an inner box 22, a preset distance is formed between the outer box 21 and the inner box 22, the preset distance is used for filling nitrogen gas into the outer box 21 and the inner box 22 to refrigerate the experimental box 2, and a plurality of support columns 28 are arranged between the outer box 21 and the inner box 22 to ensure the stability of the whole outer box 21 and the whole inner box 22 and avoid the damage of the inner box 22 due to suspension.

Specifically, the experimental box 2 is connected with an oxygen input pipe 23, an oxygen output pipe 24 and a nitrogen input pipe 25, one end of the oxygen input pipe 23 and one end of the oxygen output pipe 24 penetrate through the outer box 21 to be communicated with the inside of the inner box 22, the other end of the oxygen input pipe 23 is connected with an oxygen delivery device (such as an oxygen tank), and the other end of the oxygen output pipe 24 is connected with a negative pressure air extraction device (such as a KVP15 series negative pressure vacuum pump). The utility model discloses a connection of oxygen input tube 23 and oxygen conveyor and connection of oxygen output tube 24 and negative pressure air exhaust device enable the oxygen of inner box 22 is full of or takes out to the greatest extent.

One end of the nitrogen input pipe 25 is communicated with the outer box 21 (i.e. communicated with the gap between the outer box 21 and the inner box 22), and the other end of the nitrogen input pipe 25 is connected with a nitrogen conveying device (such as a nitrogen tank), so that the gap between the outer box 21 and the inner box 22 is filled with nitrogen, and the temperature of the experimental box 2 can be reduced to the required temperature. Further, the nitrogen input pipe 25 may be disposed at a lower portion of the outer box 21, and the specific gravity of the nitrogen may be utilized to fill the nitrogen from the bottom of the outer box upward, so as to rapidly cool the experiment box.

Furthermore, the outer walls of the oxygen input pipe 23 and the oxygen output pipe 24 are sealed with the outer box 21, so that the influence of external air on the refrigeration effect is avoided when nitrogen is filled for refrigeration. The oxygen input pipe 23, the oxygen output pipe 24 and the nitrogen input pipe 25 can be provided with electromagnetic valves 26, the electromagnetic valves 26 are electrically connected with the control device, and the working state of each electromagnetic valve can be set through the controller to control the amount of negative pressure, oxygen delivery and nitrogen charging. The controller can adopt the PLC controller commonly used (like CP series, CPM series etc. of ohm dragon), the control mode of solenoid valve 26, the control mode of cylinder etc. are prior art, and also do not the utility model discloses a protection point, no longer describe in addition.

Further, a temperature sensor (not shown) for detecting the temperature of the inner box 22 is disposed in the inner box 22, the temperature sensor is connected to the control device, and when the temperature sensor detects that the inner box 22 reaches a set temperature, the control device closes the electromagnetic valve on the nitrogen input pipe 25, so that a freezing experiment can be performed.

In order to ensure the experimental effect of oxygen deficiency and cold stimulation, one side that conveyer pipe 3 is located experimental box 2 is provided with sealing door 7, and this sealing door can adopt soft silica gel separation blade, can slide into the experimental box smoothly when the toy slides in, when taking out oxygen and defeated nitrogen, this soft silica gel separation blade can make conveyer pipe 3's export sealed again because the effect of its gravity, avoids feeding and communicates between case and the experimental box and influence the experimental result, and its implementation is simple, and is with low costs.

Furthermore, the feeding box 1, the feeder 11 and the experimental box 2 are all made of transparent materials, so that the condition of the small animals inside and the condition of the liquid inside the feeder 11 can be observed more visually from the outside.

As shown in figures 1 and 2, the experimental box 2 is provided with an openable sealing cover 27 so as to facilitate the removal of the small animals after the experiment is finished. The side of the sealing cover is provided with a silica gel sealing ring (not shown in the figure), and when the sealing cover is covered with the outer box, the outer box is isolated from the outside.

Furthermore, a suction cup device (not shown) is disposed on the feeder 11 for adhering the feeder 11 to the inner wall of the feeding box 1, so as to fix the feeder 11 and ensure that the feeder 11 will not slide or fall down when the bottom plate of the feeding box 1 inclines.

Furthermore, the upper part of the feeding box is also provided with a protective cover 16, when the protective cover 16 is opened, the feeding box is communicated with the outside, the inside of the feeding box can be kept at a preset temperature (for example, 28-30 ℃) and a preset humidity (generally, the humidity is 45% -60%), and the influence of the external environment when the newborn mice are raised in the feeding box 1 is avoided.

To sum up, the NEC experimental facility provided by the utility model comprises a feeding box, an experimental box, a conveying pipe and a control device, wherein the conveying pipe is used for feeding small animals into the experimental box, one end of the conveying pipe is connected with the feeding box, the other end of the conveying pipe is connected with the experimental box, the conveying pipe is arranged in an inclined way, the feeding box is provided with a rotatable bottom plate, a rotating shaft for rotating the bottom plate is arranged on the bottom plate, one end of the bottom plate is provided with a driving device for driving the bottom plate to rotate so as to tilt one end of the bottom plate, the experimental box is arranged at the outlet of the conveying pipe and is provided with a sealing door, when the small animals need to be fed into the experimental box, the control device controls the driving device to jack up one end of the bottom plate so as to move the other end of the bottom plate downwards, so that the bottom plate is inclined so that the small, the labor intensity of workers is greatly reduced, and the working efficiency is effectively improved.

Meanwhile, the feeding box can be controlled at constant temperature, automatic feeding can be realized as required, one experimenter is not required to feed the feed through the stomach, the feeding is not required to be carried out at regular time, and the feeding is not required to be carried out by getting up at night, so that the labor intensity is greatly reduced.

It should be understood that equivalent alterations and modifications can be made by those skilled in the art according to the technical solution of the present invention and the inventive concept thereof, and all such alterations and modifications should fall within the scope of the appended claims.

Claims

1. The utility model provides a NEC experimental facilities, its characterized in that, including be used for feeding the case of feeding, experimental box of waiting to experiment the toy, be used for sending the toy into the conveyer pipe and the controlling means of experimental box, the case is fed in the one end connection of conveyer pipe, the experimental box is connected to the other end of conveyer pipe, the conveyer pipe slope sets up, it is provided with rotatable bottom plate to feed the case, be provided with on the bottom plate and supply bottom plate pivoted axis of rotation, the one end of bottom plate is provided with and is used for driving the bottom plate to rotate the drive arrangement who makes the one end perk of bottom plate, drive arrangement is connected with the controlling means.

2. The NEC experimental apparatus of claim 1, wherein a feeder is provided in the feeding tank, the feeder has a reservoir for storing formula, the bottom of the feeder has a plurality of nipples for sucking by the fed animal, the upper portion of the feeder is provided with a liquid injection port, and the nipples and the liquid injection port are both communicated with the reservoir.

3. The NEC experimental apparatus of claim 1 further comprising a negative pressure pumping device for pumping oxygen out of the test chamber, an oxygen delivering device for supplying oxygen to the test chamber and a nitrogen delivering device for refrigerating the test chamber, wherein the oxygen delivering device, the negative pressure pumping device and the nitrogen delivering device are electrically connected to the control device.

4. The NEC experimental apparatus of claim 1 or 3, wherein the experimental box is a double-layered box, the first layer is an outer box, the second layer is an inner box, and the outer box and the inner box have a predetermined interval therebetween.

5. The NEC experimental facility of claim 4 wherein said experimental box is connected with an oxygen input tube, an oxygen output tube and a nitrogen input tube, one end of said oxygen input tube and said oxygen output tube is connected with said inner box through said outer box, the other end of said oxygen input tube is connected with an oxygen delivery device, the other end of said oxygen output tube is connected with a negative pressure air extraction device; one end of the nitrogen input pipe is communicated with the outer box, and the other end of the nitrogen input pipe is connected with the nitrogen conveying device.

6. The NEC experimental apparatus of claim 5 wherein the oxygen input tube, the oxygen output tube and the nitrogen input tube are all provided with solenoid valves, and the solenoid valves are connected with the control device.

7. The NEC experimental apparatus of claim 2 wherein said feeding chamber, said feeder and said experimental chamber are made of transparent material.

8. The NEC experimental apparatus of claim 4 wherein a temperature sensor for detecting the temperature of the inner tank is provided in the inner tank, and the temperature sensor is connected to the control device.

9. The NEC experimental apparatus of claim 1 wherein said experimental box is provided with a sealing door at an outlet of said transporting pipe.

10. The NEC experimental apparatus of claim 2 wherein said feeder is provided with a suction cup means for sticking the feeder in a feeding tank.