CN210803047U - Laser scanning device and cutting instrument - Google Patents
Laser scanning device and cutting instrument Download PDFInfo
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- CN210803047U CN210803047U CN201920950347.0U CN201920950347U CN210803047U CN 210803047 U CN210803047 U CN 210803047U CN 201920950347 U CN201920950347 U CN 201920950347U CN 210803047 U CN210803047 U CN 210803047U
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Abstract
The utility model provides a laser scanning device and a cutting instrument, wherein the laser scanning device comprises a laser and a guide rail, and the guide rail is an arc-shaped surface and is used for bearing and moving relative to a sliding part; a slider, the laser being disposed on a side of the slider facing away from the rail; one end of the elastic piece is fixed on the sliding piece, and the other end of the elastic piece is fixed; one end of the connecting piece is fixed on the sliding piece, and the other end of the connecting piece is wound on the rotating shaft; the connecting piece and the elastic piece are respectively arranged on two opposite sides of the sliding piece; a motor driving the rotation shaft to rotate. The utility model has the advantages of accurate movement and the like.
Description
Technical Field
The utility model relates to a laser scanning, in particular to laser scanning device and laser microdissection appearance.
Background
The mobile control technology is one of the key technologies for realizing Laser Capture Microdissection (LCM), the control precision of the mobile control technology determines the precision of capture and cutting of macroscopic metamorphic tissues and operation of nanoscale cells, and the volume size of the mobile control technology determines the integration level and the usability of the mobile control technology in a microscope.
The conventional LCM moves the laser by scanning a galvanometer or moving a stage. The scanning galvanometer has high scanning precision, high speed, small volume and high integration level, but the laser scanning galvanometer body rotates along the axis at high speed under the driving of a motor to change the path of a laser beam, and the highest deflection angle lens is +12.5 degrees (the +10 degrees are always a safe range) and the incidence angle cannot deviate from 45 degrees, so that a plurality of inherent problems are caused, such as:
the mobile control technology has the following defects:
1. the macroscopic large-area cutting precision is not high enough, the precision is high, and only the microscopic operation can be performed, and a plurality of laboratories can only be equipped with two devices for realizing the macroscopic and microscopic operations, so that the cost and the space are greatly increased;
2. due to the inherent properties of the vibrating mirror, the problems of mirror reflection loss, additional optical distortion and the like exist, and the operation with high precision requirement is greatly influenced;
3. because the mirror surface is partially exposed in the air, the requirement on the space cleanliness is extremely high; the scanning galvanometer structure and the control are complex and high in price;
4. moving the stage causes the sample to become poor in function, the user is inconvenient to use, and the sample is moved, causing a large error.
SUMMERY OF THE UTILITY MODEL
For solving not enough among the above-mentioned prior art scheme, the utility model provides a remove the laser scanning device that the precision is high, low-cost, need not to use galvanometer or objective table.
The utility model aims at realizing through the following technical scheme:
laser scanning device, laser scanning device includes the laser instrument, laser scanning device still includes:
the guide rail is an arc-shaped surface and is used for bearing and moving relative to the sliding part;
a slider, the laser being disposed on a side of the slider facing away from the rail;
one end of the elastic piece is fixed on the sliding piece, and the other end of the elastic piece is fixed;
one end of the connecting piece is fixed on the sliding piece, and the other end of the connecting piece is wound on the rotating shaft; the connecting piece and the elastic piece are respectively arranged on two opposite sides of the sliding piece;
a motor driving the rotation shaft to rotate.
The utility model aims at providing still that the laser microdissection appearance of using above-mentioned laser scanning device is provided, this utility model purpose can realize through following technical scheme:
the laser microdissection instrument comprises a scanning device, and the scanning device adopts the laser scanning device.
Compared with the prior art, the utility model discloses the beneficial effect who has does:
1. a galvanometer is not needed, so that the cost and the complexity are reduced;
2. the moving precision is high;
the displacement of the laser on the tissue to be cut (actual displacement or on a display) is converted into the rotation angle of the motor, and the high-precision motor improves the precision of the rotation angle, so that the high precision of the displacement is ensured;
3. by moving the laser beam rather than the stage, the user is convenient to use.
Drawings
The disclosure of the present invention will become more readily understood with reference to the accompanying drawings. As is readily understood by those skilled in the art: these drawings are only intended to illustrate the technical solution of the present invention and are not intended to limit the scope of the present invention. In the figure:
FIG. 1 is a schematic diagram of a laser microdissection instrument according to an embodiment of the present invention;
fig. 2 is a schematic structural diagram of a laser microdissection instrument according to embodiment 2 of the present invention.
Detailed Description
Fig. 1-2 and the following description depict alternative embodiments of the invention to teach those skilled in the art how to make and reproduce the invention. For the purpose of teaching the present invention, some conventional aspects have been simplified or omitted. Those skilled in the art will appreciate variations or substitutions from these embodiments that will be within the scope of the invention. Those skilled in the art will appreciate that the features described below can be combined in various ways to form multiple variations of the invention. Accordingly, the present invention is not limited to the following alternative embodiments, but is only limited by the claims and their equivalents.
Example 1:
fig. 1 schematically shows a schematic structural diagram of a laser microdissection instrument according to embodiment 1 of the present invention, and as shown in fig. 1, the laser microdissection instrument includes:
a laser scanning device, the laser scanning device comprising:
the guide rail 11 is an arc-shaped surface, such as an inwards concave three-dimensional arc-shaped surface, and is used for bearing and moving relative to the sliding part;
a slide 12, the laser 10 being arranged on a side of the slide 12 facing away from the guide rail 11;
an elastic member 22 having one end fixed to the slider and the other end fixed;
a connecting member 21 having one end fixed to the slider and the other end wound around the rotating shaft; the connecting piece and the elastic piece are respectively arranged on two opposite sides of the sliding piece;
a motor that drives the rotation shaft 31 to rotate; when the motor drives the rotating shaft to rotate in a forward direction (a reverse direction), the length of the connecting piece between the rotating shaft and the sliding piece is shortened, and the elastic piece is lengthened (shortened);
the main shaft of the objective lens is coincided with the radius of the cambered surface where the guide rail is located and is vertical to the objective table, and the circle center of a circle where the running track of the laser is located on the main shaft; the objective lens can transmit light with a specific wavelength through the coating;
the objective lens and the objective table are sequentially arranged in the light emitting direction of the laser; the object stage is used for bearing tissues to be cut;
a display for microscopic imaging of the tissue to be cut.
The working process of the laser microdissection instrument comprises the following steps:
obtaining the actual displacement Y of the laser on the tissue to be cut;
and obtaining the rotation angle theta of the rotation shaft according to the actual displacement Y, wherein the specific mode is as follows:
the center of the rotating shaft is used as the origin of coordinates, the straight line when the connecting piece and the elastic piece are collinear is the x axis, the direction parallel to the main axis of the objective lens is the z axis, and the coordinate of the circle center is (z)0,x0);RsIs the radius of the running track, R0Is the radius of the rotation axis, f is the focal length of the objective lens, LrThe length of the connecting piece is regarded as theta, and theta is the angle rotated by the rotating shaft;
the motor is controlled so that the rotation angle of the motor becomes θ.
Example 2:
according to the utility model discloses the application example of laser scanning device and method of embodiment 1.
In this application example, as shown in fig. 2, the elastic member is a spring, and the connecting member is a rope having a small deformation amount; the focus of the objective lens is the center of a circle where the running track of the laser is located; the initial position of the laser is: the position of the laser on the guide rail when the elastic member and the connecting member are collinear; the guide rail is an inward concave surface and faces the objective lens, namely the running track of the laser is a concave surface.
The working process of the laser microdissection instrument comprises the following steps:
the image of the tissue to be cut is displayed on a display in which the displacement X of the outgoing light incident on the tissue to be cuts;
Displacement X according to needsAnd XsK is a proportionality coefficient, constant; obtaining the actual displacement Y of the laser on the tissue to be cut;
and obtaining the rotation angle theta of the rotation shaft according to the actual displacement Y, wherein the specific mode is as follows:
the center of the rotating shaft is used as the origin of coordinates, the straight line when the connecting piece and the elastic piece are collinear is the x axis, the direction parallel to the main axis of the objective lens is the z axis, and the coordinate of the circle center is (z)0,x0);RsIs the radius of the running track, R0Is the radius of the rotation axis, f is the focal length of the objective lens, LrThe length of the connecting piece is regarded as theta, and theta is the angle rotated by the rotating shaft;
controlling the motor to make the rotation angle of the motor be theta;
and returning to the step (A1), circulating for a plurality of times, and enabling the displacement of the laser on the tissue to be cut to meet the requirement.
Claims (6)
1. Laser scanning device, laser scanning device includes the laser instrument, its characterized in that: the laser scanning device further includes:
the guide rail is an arc-shaped surface and is used for bearing and moving relative to the sliding part;
a slider, the laser being disposed on a side of the slider facing away from the rail;
one end of the elastic piece is fixed on the sliding piece, and the other end of the elastic piece is fixed;
one end of the connecting piece is fixed on the sliding piece, and the other end of the connecting piece is wound on the rotating shaft; the connecting piece and the elastic piece are respectively arranged on two opposite sides of the sliding piece;
a motor driving the rotation shaft to rotate.
2. Laser microdissection appearance, laser microdissection appearance includes scanning device, its characterized in that: the scanning device employs the laser scanning device of claim 1.
3. The laser microdissection instrument of claim 2, wherein: the laser microdissection instrument further comprises:
objective and objective table, objective and objective table set gradually on the light-emitting direction of laser instrument, the main shaft perpendicular to of objective the objective table.
4. The laser microdissection instrument of claim 3, wherein: the focal point of the objective lens is the center of a circle where the running track of the laser is located.
5. The laser microdissection instrument of claim 4, wherein: the laser has a concave running track and faces the objective lens.
6. The laser microdissection instrument of claim 5, wherein: the laser microdissection instrument further comprises:
a display for microscopic imaging of the tissue to be cut.
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CN201920950347.0U CN210803047U (en) | 2019-06-24 | 2019-06-24 | Laser scanning device and cutting instrument |
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CN201920950347.0U CN210803047U (en) | 2019-06-24 | 2019-06-24 | Laser scanning device and cutting instrument |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN110286011A (en) * | 2019-06-24 | 2019-09-27 | 宁波大学 | Laser scanning device, cutting device and working method |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN110286011A (en) * | 2019-06-24 | 2019-09-27 | 宁波大学 | Laser scanning device, cutting device and working method |
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