CN210774901U - Soil culture and greenhouse gas collection system - Google Patents

Abstract

The utility model discloses a soil culture and greenhouse gas collection device, which comprises a culture cavity and a cover body, wherein the upper end of the culture cavity is open, the lower end of the culture cavity is sealed, a supporting clapboard is arranged in the culture cavity, a plurality of through holes are arranged on the supporting clapboard, an air inlet is arranged at the lower part of the culture cavity, the air inlet is communicated with an air outlet of a blower outside the culture cavity, an air outlet is arranged at the upper part of the culture cavity, and the air inlet and the air outlet are both provided with valves; the gas production structure is arranged on the cover body and comprises a carrier gas inlet, a carrier gas outlet, a greenhouse gas inlet and a greenhouse gas outlet, the cover body covers the culture cavity body, the carrier gas outlet and the greenhouse gas inlet of the gas production structure are located in the culture cavity body, and the carrier gas inlet and the greenhouse gas outlet are located outside the culture cavity body. The utility model adopts the above structure, make measuring result more accurate, make the greenhouse gas of collecting more close to the true value simultaneously.

Description

Soil culture and greenhouse gas collection system

Technical Field

The invention belongs to the field of soil culture and greenhouse gas collection, and particularly relates to a soil culture and greenhouse gas collection device.

Background

Soil is an important source for the production of greenhouse gases such as carbon dioxide, methane, nitrous oxide and the like, and the soil greenhouse gases mainly come from microbial respiration, plant root respiration and soil animal respiration. The mechanism of soil greenhouse gas emission and its influencing factors are important components for the study of the global carbon and nitrogen cycle. Research has shown that the soil microbial action process is regulated by various factors such as soil texture, temperature, moisture and the like, thereby influencing greenhouse gas emission. The method for clearly setting up the emission rule of the soil greenhouse gases and the influence factors thereof is the key for scientifically knowing the influence of climate change on the greenhouse gas source/sink function of the land ecosystem, and the accurate depiction of the generation process and contribution of the soil greenhouse gases has important significance for the reasonable utilization of land and the protection of biological diversity. Therefore, scholars and research institutions at home and abroad use the soil culture technology to research the greenhouse gas emission process of soil of different ecosystems, discuss the space-time change rule of soil greenhouse gas emission, and estimate the emission amount.

The existing soil culture technology generally adopts closed culture, a soil sample is placed in a culture cavity, then ventilation culture is carried out, and gas collection is carried out after the soil sample is closed for a short time in a culture period. Such soil culture and gas production methods have various problems: firstly, due to the stacking of soil, the ventilation of the soil is poor, the microbial habitat of the soil at the bottom layer is not good, the released gas cannot penetrate through the soil at the upper layer and enter an upper sampling area, the gas collected by the air extraction device is not the gas released by all the soil, and therefore the measured discharge amount is not the gas amount actually released by the soil; secondly, the prior soil culture generally comprises the steps of scattering collected soil, removing impurities and placing the soil in a culture device, so that the structure of the soil and the activity of microorganisms in the soil are undoubtedly damaged, greenhouse gases generated by the soil are not completely close to a true value, and the authenticity of a result is influenced; finally, in the gas collection process, two methods are generally adopted, one is to extract gas from a culture container into a vacuum blood collection tube, the gas is extracted by an injector during the on-machine test, and then the gas is injected into a pipeline through a sample inlet and enters a mass spectrometer for detection, because the gas can be injected only manually, the gas can be injected in a sample less than 1 ml (the sample injection amount is only hundreds of microliters when some samples are made), because the isotope result parallelism is poor due to manual operation, and the sample injection volume is greater than 100 milliliters, the gas collection process is limited by the specification of the injector, and can not meet the sample injection amount of more than 100 milliliters.

Disclosure of Invention

In order to overcome the defects of the prior art, the invention provides a soil culture and greenhouse gas collection device, which solves the problems that in the existing soil culture, the soil permeability is poor, the greenhouse gas released by the soil cannot be really measured, and the isotope fractionation and gas transfer are easily caused in the gas collection process to cause gas leakage.

The technical scheme adopted by the invention for solving the problems is as follows:

a soil culture and greenhouse gas collection device comprises a culture cavity and a cover body, wherein the upper end of the culture cavity is open, the lower end of the culture cavity is sealed, a supporting partition plate is arranged in the culture cavity, a plurality of through holes are formed in the supporting partition plate, an air inlet is formed in the lower portion of the culture cavity and is communicated with an air outlet of a blower outside the culture cavity, an air outlet is formed in the upper portion of the culture cavity, and valves are arranged on the air inlet and the air outlet;

the gas production structure is arranged on the cover body and comprises a carrier gas inlet, a carrier gas outlet, a greenhouse gas inlet and a greenhouse gas outlet, the cover body covers the culture cavity, the carrier gas outlet and the greenhouse gas inlet of the gas production structure are located in the culture cavity, and the carrier gas inlet and the greenhouse gas outlet are located outside the culture cavity.

Further, as a preferred technical scheme, the gas production structure comprises an inner pipe, an outer pipe, an input pipe and an output pipe, wherein the inner pipe is located in the outer pipe, the lower end of the outer pipe is closed and is fixed with the inner pipe in a sealing mode, the upper end of the outer pipe is fixed with and communicated with the output pipe, the input pipe penetrates into the output pipe and is fixed with and communicated with the inner pipe, the fixing position of the input pipe and the fixing position of the output pipe are subjected to sealing treatment, and the outer pipe is provided with at least one air hole.

Further, as preferred technical scheme, still include humidity transducer, atomizer and microcontroller, humidity transducer sets up in cultivateing the cavity and be located the supporting partition board top, the atomizer sets up outside cultivateing the cavity, and the shower nozzle of atomizer stretches into in cultivateing the cavity, microcontroller's signal input part is connected with humidity transducer's signal output part, and microcontroller's control signal output part is connected with the on-off control end of atomizer.

Further, as a preferred technical scheme, a bearing ring is arranged at the upper edge of the culture cavity, an annular groove is formed in the bearing ring, and an elastic sealing ring matched with the annular groove in shape and size is filled in the annular groove.

Further, as a preferred technical scheme, the elastic sealing ring is a silicon rubber sealing ring.

Further, as a preferred technical scheme, a plurality of upper-layer through holes are formed in the cover body, lower-layer through holes corresponding to the upper-layer through holes are formed in the bearing ring, the upper-layer through holes are regular polygon stepped holes, and the lower-layer through holes are circular holes;

the novel anti-theft bolt further comprises a plurality of matched bolts and nuts, and the shape of the head of each bolt is matched with the shape and size of the upper-layer through hole.

Further, as a preferred technical scheme, a second carrier gas inlet is further arranged on the culture cavity, a three-way valve is arranged on the second carrier gas inlet, the three-way valve is provided with A, B, C three ports respectively, wherein the port A is used as an inlet, the port B and the port C are used as outlets, the port C is communicated with the culture cavity, a three-way valve is also arranged on the gas outlet, the three-way valve is provided with D, E, F three ports respectively, the port D and the port E are used as inlets, the port F is used as an outlet, the port D is communicated with the culture cavity, and the port B is communicated with the port E through a pipe.

A soil culture and gas production method comprises the following steps:

step 1: drilling soil to obtain a soil column, putting the soil column into a culture cavity for culture, keeping the air inlet state of an air inlet, and opening a cover body;

step 2: covering the cover body, closing the valves on the air inlet and the air outlet, and continuing to culture in a closed manner for a period of time;

and step 3: and introducing carrier gas into the culture cavity through the carrier gas inlet, replacing the greenhouse gas in the culture cavity, and introducing the greenhouse gas into the detection machine through the greenhouse gas outlet to finish gas collection.

Further, as a preferred technical scheme, in the step 3, when two indexes of the greenhouse gas need to be detected by injecting the sample into the detection machine twice, the first detection sample injection is performed, the ports C and D are in an open state, the ports B and E are in a closed state, the carrier gas is introduced into the culture cavity for a period of time through the second carrier gas inlet, a part of the greenhouse gas in the culture cavity is expelled and is sent into the detection machine through the gas outlet for detection, then the ports C and D are closed, the ports B and E are opened, the carrier gas is allowed to flow out along the ports B, E and F, and the first detection is completed; and then starting second detection sample introduction, keeping the port A of the second carrier gas inlet in an air inlet state all the time, opening the ports C and D, closing the ports B and E, replacing residual greenhouse gas in the culture cavity by carrier gas entering the second carrier gas inlet, and entering a detection machine for detection through the port F to finish second detection.

Further, as a preferable technical scheme, in the step 1, the height of the soil column is 1/2-2/3 of the distance from the supporting clapboard to the edge of the culture cavity.

Compared with the prior art, the invention has the following beneficial effects:

(1) according to the invention, the microcontroller, the humidity sensor and the atomizer are designed as a humidity control structure, so that the automatic regulation and control of the humidity of the soil are realized, the culture environment of the culture cavity is closer to the real environment of the soil, the measured greenhouse gas amount is more likely to the greenhouse gas amount really released by the soil, and soil culture under different environments, such as high mountain grassland, farmlands and the like, can be obtained according to the control of the microcontroller and the humidity sensor, so that more accurate test results are obtained.

(2) According to the invention, the partition plate with the vent holes is innovatively introduced and arranged in the culture cavity, and the soil is placed on the partition plate for culture, so that the greenhouse gas generated by the soil at the lowest layer can be released to the lower part of the culture cavity from the vent holes, the good ventilation of the soil is kept, the collected gas released by the soil is ensured to be as uniform as possible, the measured greenhouse gas emission flux is closer to the greenhouse gas flux released by the real habitat of the soil, and the measurement result is more real and accurate.

(3) According to the invention, the soil is drilled to obtain the soil column, and the soil column is directly placed in the culture cavity for culture, compared with the traditional scattered soil culture, the soil culture method has the advantages that the original state of the soil is better kept, and the original living environment of microorganisms in the soil is kept to the maximum extent, so that the measured greenhouse gas emission flux is closer to the greenhouse gas flux released under the real environment of the soil, and the measurement result is more real and accurate.

(4) Compared with the traditional mode, the gas collection device does not involve gas transfer, so that gas leakage possibly generated in the transfer process is avoided, and the problem of isotope fractionation caused in the pumping process of a traditional injector is also avoided.

(5) According to the invention, by designing the second carrier gas inlet and combining with the gas outlet, when two index tests are carried out, only about half of greenhouse gas in the culture cavity 1 needs to be sent into a detection machine in sequence, namely two indexes can be detected by using only one culture cavity, and in the two tests, the carrier gas is uninterrupted, so that the normal and continuous detection is ensured, the waveform data obtained on a mass spectrometer is relatively continuous, and the test result can be conveniently and visually observed and read; meanwhile, on the basis of normal operation of the carrier gas, the greenhouse gas in the culture cavity is not diluted, a sample which is relatively consistent with the first index detection is provided for the second index detection, compared with two bottles of different greenhouse gases, the method ensures the consistency of the greenhouse gas tested in two times before and after the composition, the tested two index results have higher reliability and higher test value, and simultaneously, compared with a mode of simultaneously culturing two bottles of greenhouse gas to test two indexes, the method reduces the test cost to a certain extent.

Drawings

FIG. 1 is a schematic view of the overall structure of embodiment 1 of the present invention;

FIG. 2 is a schematic view of the overall structure of embodiment 2 of the present invention;

FIG. 3 is a schematic view of a structure in soil culture;

FIG. 4 is a schematic structural view of an elastic sealing ring;

FIG. 5 is a schematic view of the positional relationship between the cover, the elastic sealing ring and the receiving ring;

FIG. 6 is a schematic structural view of a bolt and a nut;

FIG. 7 is a schematic diagram of the control relationship between the humidity sensor, the microcontroller, and the atomizer;

FIG. 8 is a schematic structural view of a gas production structure;

fig. 9 is a schematic view of the overall structure of embodiment 3 of the present invention.

The names corresponding to the labels in the figure are: 1. the device comprises a culture cavity, 2, an air inlet, 3, an air outlet, 4, a cover body ring, 5, a supporting partition board, 6, a gas production structure, 7, a through hole, 8, a bearing ring, 9, an elastic sealing ring, 10, an annular groove, 11, a humidity sensor, 12, an atomizer, 13, an upper layer through hole, 14, a lower layer through hole, 15, a bolt, 16, a nut, 17 and a second carrier gas inlet.

Detailed Description

The present invention will be described in further detail with reference to examples and drawings, but the present invention is not limited to these examples.

Example 1

As shown in fig. 1, a soil cultivation and greenhouse gas collection device according to a preferred embodiment of the present invention includes a cultivation chamber 1 and a cover 4, the cultivation chamber 1 has an open upper end and a sealed lower end, a supporting partition plate 5 is disposed in the cultivation chamber 1, the supporting partition plate 5 is provided with a plurality of through holes 7, the size of the through holes 7 is determined based on that the soil does not leak downwards, generally speaking, the cultivated soil has a certain wettability and is not as fine as dry sand, therefore, as long as the aperture of the through holes 7 is not too large, the downward leakage generally does not occur, which is easily implemented by those skilled in the art; the lower part of the culture cavity 1 is provided with an air inlet 2, the air inlet 2 is communicated with an air outlet of an air blower (not shown in the figure) outside the culture cavity 1, the upper part of the culture cavity 1 is provided with an air outlet 3, and the air inlet 2 and the air outlet 3 are both provided with valves.

In this embodiment, the culture cavity 1 is used for providing a culture environment for soil, the culture cavity 1 preferably adopts a long cylindrical structure, the culture cavity 1 and the cover 4 preferably adopt organic glass, the cover 4 is used for opening and closing the culture cavity 1, the cover 4 and the culture cavity 1 can be connected in a hinged manner, the cover 4 can be opened and closed, and after the culture cavity 1 is closed by the cover 4, the cover 4 and the culture cavity 1 can be compressed and sealed in a manner of placing a heavy object on the cover 4; the cover body 4 and the culture cavity 1 can also adopt threaded connection, similar to a bottle cap; in addition, the cover body 4 and the culture cavity 1 do not need to be connected, when the culture cavity 1 is closed, the cover body 4 is directly covered on the opening of the culture cavity 1, the opening is completely covered, and then a heavy object is placed on the cover body 5, so that the sealing between the cover body 5 and the culture cavity 1 is realized.

In the embodiment, the air blower introduces air into the culture cavity 1 through the air inlet 2 and flows out of the air outlet 3, and the plurality of through holes 7 in the supporting partition plate 5 can greatly enhance the ventilation property in the culture cavity 1, so that greenhouse gas generated by the soil at the lowest layer can be released to the upper space of the culture cavity 1 from the through holes 7, the gas collection is facilitated, the measured greenhouse gas emission flux is closer to the greenhouse gas flux released by the soil in the real growth environment, and the measurement result is more real and accurate.

The embodiment further comprises a gas production structure 6, wherein the gas production structure 6 is arranged on the cover body 4, the gas production structure 6 comprises a carrier gas inlet, a carrier gas outlet, a greenhouse gas inlet and a greenhouse gas outlet, the cover body 4 covers the culture cavity 1, the carrier gas outlet and the greenhouse gas inlet of the gas production structure 6 are both positioned in the culture cavity 1, and the carrier gas inlet and the greenhouse gas outlet are positioned outside the culture cavity 1. The carrier gas inlet is communicated with an output port of an external carrier gas output device (not shown in the figure), the carrier gas enters the culture cavity 1 through the carrier gas inlet and the carrier gas outlet, and greenhouse gas generated by soil in the culture cavity 1 is expelled out of the culture cavity 1 through the greenhouse gas inlet and the greenhouse gas outlet and is sent to a detection machine. This embodiment is through adopting gas production structure 6 to realize greenhouse gas's collection, censorship, has abandoned traditional syringe mode of bleeding, can once only gather required amount's greenhouse gas to avoided traditional syringe to bleed many times and can cause the condition of isotope fractionation to take place, traditional syringe is subject to its specification, when the air input is great (being greater than 100 ml), need divide many times to bleed, will cause the isotope fractionation this moment, influences the testing result.

As shown in fig. 8, the gas production structure 6 of the present embodiment may preferably adopt the following structure: the gas production structure 6 comprises an inner pipe 61, an outer pipe 62, an input pipe 63 and an output pipe 64, wherein the inner pipe 61 is positioned in the outer pipe 62, the lower end of the outer pipe 62 is closed and is fixed with the inner pipe 61 in a sealing manner, the upper end of the outer pipe 62 is fixed and communicated with the output pipe 64, the input pipe 63 penetrates into the output pipe 64 and is fixed and communicated with the inner pipe 61, the fixing position of the input pipe 63 and the output pipe 64 is processed in a sealing manner, and the outer pipe 62 is provided with at least one air hole 65. The inner tube 61 and the outer tube 62 form a sleeve structure, wherein the inner tube 61 is used for introducing carrier gas into the culture cavity 1, a gap between the inner tube 61 and the outer tube 62 is used as a flow channel of greenhouse gas, a tube opening of the input tube 63 is used as a carrier gas inlet, a lower end opening of the inner tube 61 is used as a carrier gas outlet, a gas hole 65 on the outer tube 62 is used as a greenhouse gas inlet, a tube opening of the output tube 64 is used as a greenhouse gas outlet, a tube opening of the output tube 64 is directly and hermetically connected with a gas inlet of a detection machine, and the carrier gas entering the culture cavity 1 in the inner tube 61 just drives the greenhouse gas in the culture cavity 1 into the outer tube 62, so that the replacement of the carrier gas on.

In the existing gas collection link, gas is generally extracted from a culture container to a vacuum blood collection tube, when the device is operated to test, the gas is extracted by an injector and injected into a pipeline through a sample inlet to enter a mass spectrometer for detection, in the mode, the gas can be injected only manually, when the sample is introduced with the volume of less than 1 ml (the sample volume is only hundreds of microliters when some samples are made), the isotope result parallelism is poor due to manual operation, and when the sample volume is more than 100 milliliters, the gas is limited by the specification of the injector, and the sample volume of more than 100 milliliters can not be met. This embodiment is through adopting above-mentioned gas production structure to replace traditional syringe, even be less than 1 ml advance a kind and also need not manual extraction, and then can avoid leading to the poor condition of isotope result parallelism to take place, and to advancing a kind volume and be greater than 100 ml when rise, adopt gas to the detection machine once equally, can not take place like syringe extraction, transfer many times, avoided isotope fractionation, the condition of gas leakage.

It is understood that the sizes and materials of the inner tube 61, the outer tube 62, the input tube 63, the output tube 64 and the air holes 65 of the present embodiment can be designed and selected according to the requirement, and are not particularly limited.

Example 2

As shown in fig. 2, in order to better provide a relatively real cultivation environment for soil, in this embodiment, a humidity sensor 11, an atomizer 12 and a microcontroller may be further added, the humidity sensor 11 is disposed in the cultivation cavity 1 and located above the supporting partition plate 5, the atomizer 12 is disposed outside the cultivation cavity 1, a nozzle of the atomizer 12 extends into the cultivation cavity 1, a contact portion between the nozzle and the cultivation cavity 1 is sealed, a signal input end of the microcontroller is connected with a signal output end of the humidity sensor 11, a control signal output end of the microcontroller is connected with a switch control end of the atomizer 12, start and stop control of the atomizer 12 by the microcontroller realizes humidity adjustment of soil, so that soil in the cultivation cavity 1 is always in the relatively real environment, and further test data is closer to a real value.

As shown in fig. 7, the principle of implementing humidity control in this embodiment is as follows: humidity sensor 11 gathers the humidity of soil in cultivateing cavity 1 in real time to signal transmission who will gather gives microcontroller, and microcontroller sends control signal to atomizer 12 respectively according to the signal of receiving, controls the start-up and the off-state of switching atomizer 12. It should be noted that, the humidity sensor, the microcontroller, the atomizer, and the blower in this embodiment are all mature existing structures, and therefore, the above structures in this embodiment are all implemented by the existing structures, and the control process thereof is easily understood and implemented by those skilled in the art, and therefore, the specific models and operating principles of the humidity sensor, the microcontroller, the atomizer, and the blower are not described herein in detail, and those skilled in the art can implement the object of the present invention by combining with the common general knowledge in the art on the basis of knowing the structure and the connection relationship provided in this embodiment.

It should be noted that, although the blower and the microcontroller are not shown in fig. 1 and fig. 2, but do not affect understanding of the technical solution, on the basis of knowing the text description part of the present embodiment, a person skilled in the art can understand the technical solution and the implementation process of the present invention with reference to fig. 1 and fig. 2, for the present embodiment, even though specific positions of the blower and the microcontroller are not shown in fig. 1 and fig. 2, the positions and the specific connection relationships of the blower and the microcontroller are easy to understand and implement for a person skilled in the art, the blower and the microcontroller may be installed at any position outside the culture chamber 1, and on the premise that normal operation of the blower and the microcontroller is not affected, the technical solution given in the present embodiment is clear and complete.

In this embodiment, realized the automatically regulated control to the humidity of soil through microcontroller, humidity transducer, atomizer, air-blower, the cultivation environment that makes the cultivation cavity more is close to the real environment of soil to the greenhouse gas concentration who surveys more tends to the real release of soil greenhouse gas concentration, and can obtain the soil cultivation under the different environment according to microcontroller, humidity transducer's control, for example mountain meadow, plain meadow etc. thereby obtain comparatively accurate test result.

In addition, because the cover 4 is in the open state, the blower of the air inlet 2 is in the direct air intake state, and the air circulation is good when the culture chamber 1 is used for soil culture, the temperature itself in the culture chamber 1 is not too high, so that the condition of excessive temperature of the soil is not needed, and the humidity of the soil is only conditioned by using the humidity sensor in the embodiment.

As shown in fig. 1, fig. 2 and fig. 4, in order to better realize the sealing between the cover 5 and the culture cavity 1, in this embodiment, a receiving ring 8 may be further disposed at the upper edge of the culture cavity 1, preferably, the outer diameter of the receiving ring 8 is equal to the outer diameter of the cover 4, the receiving ring 8 and the culture cavity 1 may be integrally formed, an annular groove 10 is disposed on the receiving ring 8, an elastic sealing ring 9 matching with the shape and size of the annular groove 10 is filled in the annular groove 10, the thickness of the elastic sealing ring 9 is greater than the depth of the annular groove 10, when the cover 4 covers the culture cavity 1, the lower bottom surface of the cover 4 contacts and presses the elastic sealing ring 9, the elastic sealing ring 9 realizes the sealing between the cover 4 and the receiving ring 8, and further realizes the sealing between the cover 4 and the culture cavity 1. It can be understood that, because of the receiving ring 8, after the cover 4 is placed on the receiving ring 8, the culture chamber 1 can be sealed by the cover 4 by placing a heavy object on the cover, and the cover 4 and the receiving ring 8 can be fastened by a plurality of clips, so that the cover is convenient to fasten and take down. The elastic sealing ring 9 of this implementation preferably adopts the silicon rubber sealing ring, and the silicon rubber sealing ring is current structure, so no longer do too much to its specific structure and material give unnecessary details.

As shown in FIGS. 5 and 6, the present embodiment provides another solution to the problem of the sealing between the cover 4 and the culture chamber 1, which is specifically as follows: the cover body 4 is provided with a plurality of upper-layer through holes 13, the bearing ring 8 is provided with lower-layer through holes 14 corresponding to the upper-layer through holes 13, the upper-layer through holes 13 are regular polygon stepped holes, the lower-layer through holes 14 are circular holes, and the upper-layer through holes 13 and the lower-layer through holes 14 are preferably 4 and are in one-to-one correspondence from top to bottom; in addition, the device also comprises bolts 15 and nuts 16 matched with the number and the shape of the upper layer through holes 13, and the head shape of the bolt 15 is matched with the shape and the size of the upper layer through holes 13. During assembly, after the cover body 4 is placed on the bearing ring 8, the upper layer through hole 13 and the lower layer through hole 14 are aligned, then the bolt 15 is inserted, the head of the bolt 15 is clamped in the upper layer through hole 13, then the nut 16 is sleeved from the lower part and rotates upwards, the cover body 4 and the bearing ring 8 are locked and sealed, and when the cover body 4 needs to be taken down, the nut 16 only needs to be rotated downwards, so that the cover body 4 can be taken down after the nut 16 is separated from the bolt 15.

Example 3

In the process of detecting greenhouse gases, two indexes of the greenhouse gases need to be respectively detected sometimes, and the existing detection mode is that two bottles of greenhouse gases obtained under the same culture condition are respectively detected to achieve the purpose of detecting the two indexes. Obviously, such a method has certain problems, because even under the same culture conditions, the culture soil cannot be completely the same, the released greenhouse gases cannot be completely the same, and the consistency of two indexes measured by two bottles of different greenhouse gases cannot be guaranteed, obviously, the reliability and accuracy of the test result are affected, and meanwhile, when two indexes are tested each time, at least two bottles of greenhouse gases obtained under the same conditions need to be cultured, so that the test cost is increased to a certain extent; if the same bottle of greenhouse gas is adopted, the carrier gas always keeps in an air inlet state in the sampling and detecting processes, obviously, the continuously entering carrier gas can dilute and take away the greenhouse gas in the bottle during the first detection, and the greenhouse gas in the bottle is basically replaced by the carrier gas after the first detection is finished, so that the test value is lost.

To solve the above problem, as shown in FIG. 9, in this embodiment, on the basis of embodiment 2, a second carrier gas inlet 17 is further provided on the culture chamber 1, and a three-way valve having A, B, C three ports is provided on the second carrier gas inlet 17, wherein port A is used as an inlet, port B and port C are used as outlets, port C is communicated with the culture chamber 1, and a three-way valve having D, E, F three ports is also provided on the gas outlet 3, wherein port D and port E are used as inlets, port F is used as an outlet, port D is communicated with the culture chamber 1, and port B and port E are communicated through a pipe, wherein a latex pipe can be used as the pipe.

The working principle of the embodiment is as follows: when two indexes of greenhouse gas need to be detected by feeding the sample into the detection machine twice, the first detection sample feeding is firstly carried out, the ports C and D are in an open state, the ports B and E are in a closed state, carrier gas is fed into the culture cavity 1 through the second carrier gas inlet 17 for a period of time, the time for feeding the carrier gas can be obtained according to a plurality of tests in advance, for example, how long the carrier gas fed into the second carrier gas inlet 17 can completely exhaust the greenhouse gas in the culture cavity 1, the last measurement is assumed to be 10 minutes, and after the carrier gas is fed into the culture cavity 1 for a period of time, 50% of the greenhouse gas in the culture cavity 1 can be exhausted, the 3 minutes is assumed, then the time for feeding the carrier gas into the culture cavity 1 for the first time is 3 minutes, at the moment, just half of the greenhouse gas in the culture cavity 1 is fed into the detection machine, and when a part of the greenhouse gas in the culture cavity 1 is exhausted, the carrier gas is sent into a detection machine for detection through the gas outlet 3, then the port C and the port D are closed, the port B and the port E are opened, the carrier gas flows out along the port B, the port E and the port F, the first detection is completed, the carrier gas is kept uninterrupted in the detection process, and the concentration of the greenhouse gas in the culture cavity 1 is not influenced; and then, starting second detection sample injection, keeping the port A of the second carrier gas inlet 17 in an air inlet state all the time, opening the ports C and D, closing the ports B and E, replacing residual greenhouse gas in the culture cavity 1 by carrier gas entering the second carrier gas inlet 17, and entering a detection machine for detection through the port F to finish second detection.

The embodiment well solves the problem of two index tests, and the two tests sequentially send about half of the greenhouse gas in the culture cavity 1 into the detection machine, so that two indexes are measured by using only one culture cavity, the carrier gas is not interrupted in the two tests, the normal and continuous detection is ensured, the waveform data obtained on the mass spectrometer is relatively continuous, and the test result can be conveniently and visually observed and read; this embodiment detects this section of time of appearance after the appearance at first index and to before the appearance is detected to the second index, second carrier gas entry remains the state of admitting air all the time, guaranteed that the detection is normal, go on in succession, and this embodiment has kept this section of time's carrier gas normal current basis ingeniously, through the mode of circumventing, avoided causing the dilution to the greenhouse gas in cultivateing cavity 1, for the second index detects the sample that provides and detect relatively unanimity with first index, for two different greenhouse gas of bottle, this embodiment ensures the uniformity of the greenhouse gas of twice test before and after two in composition, two index results tested have more reliability, have more experimental value.

In this embodiment, a method for soil cultivation and gas production is provided by combining the soil cultivation apparatus, which specifically includes the following steps:

step 1: drilling soil to obtain a soil column, putting the soil column into the culture cavity 1 for culture, and keeping the air inlet state of the air inlet 2 and the cover body 4 in an open state as shown in figure 3; the soil column is drilled by a tool, the tool is of an existing structure, the diameter of the soil column is slightly smaller than the inner diameter of the culture cavity 1, meanwhile, the size of the soil column is preferably that the soil column is not scraped and touched by a humidity sensor and an atomizer sprayer in the culture cavity, in addition, the height of the soil column is not too high or too low, and the height of the soil column is preferably 1/2-2/3 of the distance from the supporting partition plate 5 to the edge of the culture cavity 1;

step 2: covering the cover body 4, closing the valves on the air inlet 2 and the air outlet 3, and continuing to culture in a closed manner for a period of time, wherein the period of time is generally 4 hours; before the closed culture, the cover body 4 is in an open state, ventilation is kept, and the closing is started only four hours before gas collection;

and step 3: and introducing carrier gas into the culture cavity 1 through a carrier gas inlet, replacing the greenhouse gas in the culture cavity 1, and introducing the greenhouse gas into a detection machine through a greenhouse gas outlet to finish gas collection.

In the step 3, when two samples need to be introduced into the detection machine twice to detect two indexes of the greenhouse gas, the first detection sample introduction is firstly carried out, the ports C and D are in an open state, the ports B and E are in a closed state, the carrier gas is introduced into the culture cavity 1 through the second carrier gas inlet 17 for a period of time, a part of the greenhouse gas in the culture cavity 1 is expelled and is sent into the detection machine through the gas outlet 3 for detection, then the ports C and D are closed, the ports B and E are opened, the carrier gas is allowed to flow out along the ports B, E and F, and the first detection is completed; and then, starting second detection sample injection, keeping the port A of the second carrier gas inlet 17 in an air inlet state all the time, opening the ports C and D, closing the ports B and E, replacing residual greenhouse gas in the culture cavity 1 by carrier gas entering the second carrier gas inlet 17, and entering a detection machine for detection through the port F to finish second detection.

It should be noted that helium is preferably used as the carrier gas in the above embodiments, and other gases may be used as the carrier gas in case of meeting the requirement.

As described above, the present invention can be preferably realized.

The foregoing is only a preferred embodiment of the present invention, and the present invention is not limited thereto in any way, and any simple modification, equivalent replacement and improvement made to the above embodiment within the spirit and principle of the present invention still fall within the protection scope of the present invention.

Claims (7)

1. The soil cultivation and greenhouse gas collection device is characterized by comprising a cultivation cavity (1) and a cover body (4), wherein the upper end of the cultivation cavity (1) is open, the lower end of the cultivation cavity is sealed, a supporting partition plate (5) is arranged in the cultivation cavity (1), a plurality of through holes (7) are formed in the supporting partition plate (5), an air inlet (2) is formed in the lower portion of the cultivation cavity (1), the air inlet (2) is communicated with an air outlet of a blower outside the cultivation cavity (1), an air outlet (3) is formed in the upper portion of the cultivation cavity (1), and valves are arranged on the air inlet (2) and the air outlet (3);

still include gas production structure (6), gas production structure (6) set up on lid (4), and gas production structure (6) include carrier gas entry, carrier gas export, greenhouse gas entry and greenhouse gas export, lid (4) lid back on cultivateing cavity (1), the carrier gas export of gas production structure (6), greenhouse gas entry all are located cultivates cavity (1), and carrier gas entry, greenhouse gas export are located outside cultivateing cavity (1).

2. The soil culture and greenhouse gas collection device of claim 1, wherein the gas collection structure (6) comprises an inner pipe (61), an outer pipe (62), an input pipe (63) and an output pipe (64), the inner pipe (61) is located in the outer pipe (62), the lower end of the outer pipe (62) is closed and is fixed with the inner pipe (61) in a sealing manner, the upper end of the outer pipe (62) is fixed and communicated with the output pipe (64), the input pipe (63) penetrates into the output pipe (64) and is fixed and communicated with the inner pipe (61), the fixing position of the input pipe (63) and the output pipe (64) is sealed, and the outer pipe (62) is provided with at least one air hole (65).

3. A soil culture and greenhouse gas collection system according to claim 1, further comprising a humidity sensor (11), an atomizer (12) and a microcontroller, wherein the humidity sensor (11) is disposed in the culture chamber (1) and above the supporting partition (5), the atomizer (12) is disposed outside the culture chamber (1), a nozzle of the atomizer (12) extends into the culture chamber (1), a signal input end of the microcontroller is connected with a signal output end of the humidity sensor (11), and a control signal output end of the microcontroller is connected with a switch control end of the atomizer (12).

4. A soil culture and greenhouse gas collection device according to claim 1, wherein the upper edge of the culture chamber (1) is provided with a receiving ring (8), the receiving ring (8) is provided with an annular groove (10), and the annular groove (10) is filled with an elastic sealing ring (9) matched with the shape and size of the annular groove (10).

5. A soil cultivation and greenhouse gas collection device as claimed in claim 4, wherein the elastic sealing ring (9) is a silicone rubber sealing ring.

6. A soil cultivation and greenhouse gas collection device as claimed in claim 4, wherein the cover (4) is provided with a plurality of upper through holes (13), the receiving ring (8) is provided with lower through holes (14) corresponding to the upper through holes (13), the upper through holes (13) are regular polygon stepped holes, and the lower through holes (14) are circular holes;

the novel anti-theft bolt is characterized by further comprising a plurality of matched bolts (15) and nuts (16), wherein the head shapes of the bolts (15) are matched with the shapes and sizes of the upper-layer through holes (13).

7. A soil culture and greenhouse gas collection device as claimed in any one of claims 1-6, wherein the culture chamber (1) is further provided with a second carrier gas inlet (17), the second carrier gas inlet (17) is provided with a three-way valve having A, B, C ports, wherein port A is an inlet port, port B and port C are outlet ports, and port C is connected to the culture chamber (1), the gas outlet (3) is also provided with a three-way valve having D, E, F ports, wherein port D and port E are inlet ports, port F is an outlet port, port D is connected to the culture chamber (1), and port B and port E are connected by a pipe.

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