CN210746907U

CN210746907U - Cell cryopreservation device for reproductive embryonic stem cells

Info

Publication number: CN210746907U
Application number: CN201921311960.4U
Authority: CN
Inventors: 王桂丽; 张伟; 徐万鹏
Original assignee: Weifang Medical University
Current assignee: Weifang Medical University
Priority date: 2019-08-14
Filing date: 2019-08-14
Publication date: 2020-06-16
Anticipated expiration: 2029-08-14

Abstract

The utility model discloses a cell cryopreservation device for reproductive embryonic stem cell relates to the relevant technical field of reproductive embryonic stem cell, include the casing and deposit the case, the right side fixed mounting of casing has the touch-sensitive screen, deposit case fixed mounting in the inside of casing, be provided with the lid directly over depositing the case. The utility model discloses it does not loosen to press adjusting button to make motor drive deposit a plurality of storage tubes of incasement use the pivot as the center rotation, and can observe the condition of the inside stem cell of transparent form storage tube through glass, facilitate the use, and conveniently take out or install the storage tube, thereby make things convenient for taking out or placing of stem cell, easy operation is taking out or installing the in-process of certain storage tube, can not influence the cryopreserving effect of other storage tubes.

Description

Cell cryopreservation device for reproductive embryonic stem cells

Technical Field

The utility model relates to a reproductive embryo stem cell correlation technique field, in particular to a cell cryopreservation device for reproductive embryo stem cell and control system thereof.

Background

Currently, stem cells are a more specialized cell that can develop into other cells. In particular embryonic stem cells, germ embryonic stem cells develop into any cell, such as neural cells, cardiac myocytes, and the like. Stem cell reproduction can therefore be used to repair damaged organs of the human body and treat many diseases that are otherwise refractory to treatment.

In stem cell clinical research, can not leave the save of stem cell, stem cell is deposited in the cryopreservation intraductal and is placed in the case of preserving usually, and current refrigerating plant is when taking out a cryopreservation pipe as required, and the case of preserving is opened completely to the cryopreservation effect of other cryopreservation intraductal stem cells of influence preservation incasement, it is very inconvenient to get and put, and current save set security is very low, causes the stem cell to be stolen easily, and intelligent degree is low.

Therefore, it is necessary to provide a cell cryopreservation apparatus for reproductive embryonic stem cells and a control system thereof to solve the above problems.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

The utility model provides a not enough to prior art, the utility model provides a cell cryopreservation device and control system for procreation embryonic stem cell has solved the problem of proposing in the background art.

(II) technical scheme

In order to achieve the above purpose, the utility model discloses a following technical scheme realizes:

a cell cryopreservation device for reproductive embryonic stem cells comprises a shell and a storage box, wherein a touch screen is fixedly arranged on the right side of the shell, the storage box is fixedly arranged in the shell, a cover body is arranged right above the storage box, a liquid nitrogen tank is inserted at the top of the left side of the shell, a delivery pipe is communicated between the liquid nitrogen tank and the storage box, the delivery pipe is fixedly provided with an electromagnetic valve, the left side of the bottom of the storage box is fixedly provided with a temperature sensor, a controller is fixedly arranged at the bottom of the right side in the shell, a taking hole is arranged at the right side of the top wall of the storage box, the bolt is fixedly arranged in the taking and placing hole, the middle of the bottom wall of the storage box is movably provided with a rotating shaft, the top end of the rotating shaft is fixedly provided with a fixed sleeve, the outer side of the fixed sleeve is fixedly connected with a plurality of supports, and one end of each support, far away from the fixed sleeve, is fixedly provided with a placing frame;

the plug bush has been placed to the inside of rack, the storage cylinder has been pegged graft at the top of plug bush, the lid is installed to the bottom opening part of storage cylinder, the top fixed mounting of storage cylinder has the ball, the equal fixed mounting in both sides bottom of plug bush has the guide block, the guide slot has been seted up to the inside wall of rack, guide block and guide slot sliding connection, fixedly connected with spring between the interior roof of guide block and guide slot, the top surface embedding of depositing the case has glass, glass's inboard is provided with adjustment button.

Optionally, the lid passes through the intelligence lock and is connected with the casing, fixedly connected with handle in the middle of the top of lid, the lid is close to top one side fixed mounting of intelligence lock and has the code keypad.

Optionally, a battery is fixedly mounted at the bottom of the left side of the inside of the shell, and a charging interface is arranged on the left side of the battery.

Optionally, a driven gear is fixedly mounted at the bottom end of the rotating shaft, and the driven gear is meshed with a gear on the output end of the motor.

Optionally, a plurality of said stents are arranged in a circumferential array;

the placing frame is a U-shaped frame.

Optionally, the insert sleeve is cylindrical;

the diameter value of the top end of the storage cylinder, the outer diameter value of the plug bush and the inner diameter value of the taking hole are equal.

Optionally, the guide block is matched with the guide groove;

the adjusting button is fixedly arranged on the top surface of the storage box.

The utility model provides a control system for freezing save set of cell of reproduction embryonic stem cell, includes main control CPU, power module, information acquisition module, display module, storage module and execution module, main control CPU's input is all connected to power module and information acquisition module's output electricity, the output of battery is connected to power module's input electricity, temperature sensor's output is connected to information acquisition module's input electricity, main control CPU's output is connected to display module, storage module and execution module's input electricity, touch-sensitive screen, password keyboard and adjustment button's output is connected to main control CPU's input electricity, display module's output is connected with the input electricity of touch-sensitive screen.

Optionally, the main control CPU, the power supply module, the information acquisition module, the display module, the storage module, and the execution module constitute a controller.

Optionally, the output end of the execution module is electrically connected with the input ends of the intelligent lock, the electromagnetic valve and the motor.

(III) advantageous effects

The utility model provides a cell cryopreservation device for reproductive embryonic stem cell and control system thereof possesses following beneficial effect:

(1) the utility model discloses a temperature sensor real-time detection deposits the temperature of incasement and shows through touch-sensitive screen display, and the staff must open intelligent lock through the correct password of code keypad input to open the lid, strengthened the security of device, prevent that non-staff from stealing to get the stem cell.

(2) The utility model discloses the adjustment button is moved in the pressure does not loosen to make a plurality of storage tubes that motor drive deposited the incasement use the pivot as the center rotation, and can observe the condition of the inside stem cell of storage tube of transparence through glass, facilitate the use, and conveniently take out or install the storage tube, thereby make things convenient for taking out or placing of stem cell, easy operation is taking out or installing the in-process of certain storage tube, can not influence the cryopreserved effect of other storage tubes.

Drawings

Fig. 1 is a schematic view of the appearance structure of the present invention;

FIG. 2 is a schematic sectional view of the housing of the present invention;

fig. 3 is a schematic view of the top view structure of the fixing sleeve of the present invention;

FIG. 4 is a schematic cross-sectional view of the insert sleeve of the present invention;

FIG. 5 is a schematic view of the top surface of the storage box of the present invention;

fig. 6 is a block diagram of the system of the present invention;

fig. 7 is a block diagram of an execution module according to the present invention.

In the figure: the intelligent storage device comprises a shell 1, a touch screen 2, a storage box 3, a cover body 4, a handle 5, a password keyboard 6, an intelligent lock 7, a liquid nitrogen tank 8, a conveying pipe 9, an electromagnetic valve 10, a temperature sensor 11, a battery 12, a charging interface 13, a controller 14, a taking and placing hole 15, a bolt 16, a rotating shaft 17, a driven gear 18, a motor 19, a fixing sleeve 20, a support 21, a placing frame 22, an inserting sleeve 23, a storage cylinder 24, a cover 25, a ball 26, a guide block 27, a guide groove 28, a spring 29, glass 30, an adjusting button 31, a main control CPU32, a power supply module 33, an information acquisition module 34, a display module 35, a storage module 36 and an execution module 37.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", and the like, indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

In the present invention, unless otherwise expressly stated or limited, the terms "disposed," "mounted," "connected," and "fixed" are to be construed broadly, e.g., as meaning either a fixed connection or a removable connection; may be a mechanical connection; may be directly connected or indirectly connected through an intermediate. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature.

According to as shown in fig. 1-7, the utility model provides a technical scheme:

a cell cryopreservation device for reproductive embryonic stem cells comprises a shell 1 and a storage box 3, wherein a touch screen 2 is fixedly installed on the right side of the shell 1, the storage box 3 is fixedly installed inside the shell 1, a cover body 4 is arranged right above the storage box 3, the cover body 4 is connected with the shell 1 through an intelligent lock 7, a handle 5 is fixedly connected in the middle of the top of the cover body 4, a code keypad 6 is fixedly installed on one side, close to the top of the intelligent lock 7, of the cover body 4, when the cover body 4 needs to be opened, a worker must input a correct code through the code keypad 6 to open the intelligent lock 7, so that the cover body 4 is opened, a liquid nitrogen tank 8 is inserted into the top of the left side of the shell 1, a conveying pipe 9 is communicated between the liquid nitrogen tank 8 and the storage box 3, the liquid nitrogen tank 8 is filled into the storage box 3 through the conveying pipe 9, an electromagnetic valve 10 is fixedly installed on the, the temperature sensor 11 detects the temperature in the storage box 3 in real time and displays the temperature through the touch screen 2, the battery 12 is fixedly installed at the bottom of the left side inside the shell 1, the battery 12 provides electric energy for the device, the charging interface 13 is arranged at the left side of the battery 12, the controller 14 is fixedly installed at the bottom of the right side inside the shell 1, the right side of the top wall of the storage box 3 is provided with the taking hole 15, the bolt 16 is fixedly installed in the taking hole 15, the rotating shaft 17 is movably installed in the middle of the bottom wall of the storage box 3, the driven gear 18 is fixedly installed at the bottom end of the rotating shaft 17, the driven gear 18 is meshed with the gear at the output end of the motor 19, the fixed sleeve 20 is fixedly installed at the top end of the rotating shaft 17, the plurality of supports 21 are fixedly connected with a plurality of supports 21 in a circumferential array arrangement, a placing rack, the rotating shaft 17 drives the placing rack 22 to move through the bracket 21;

the placing frame 22 is a U-shaped frame, the inserting sleeve 23 is placed in the placing frame 22, the top of the inserting sleeve 23 is inserted with the storage barrel 24, the storage barrel 24 is a transparent barrel, the opening at the bottom end of the storage barrel 24 is provided with the cover 25, the top end of the storage barrel 24 is fixedly provided with the ball 26, the inserting sleeve 23 is cylindrical, the diameter value of the top end of the storage barrel 24, the outer diameter value of the inserting sleeve 23 and the inner diameter value of the taking and placing hole 15 are equal, the bottoms of the two sides of the inserting sleeve 23 are fixedly provided with the guide blocks 27, the inner side wall of the placing frame 22 is provided with the guide grooves 28, the guide blocks 27 are matched with the guide grooves 28, the guide blocks 27 are in sliding connection with the guide grooves 28, the springs 29 are fixedly connected between the guide blocks 27 and the inner top walls of the guide grooves 28, the springs 29 in the placing frame 22 are in a stretching deformation state, the glass 30 is embedded in the, the ball 26 at the top end of the storage cylinder 24 is attached to the top wall of the storage box 3 to slide, the condition of stem cells in the transparent storage cylinder 24 can be observed through the glass 30, the inner side of the glass 30 is provided with an adjusting button 31, the adjusting button 31 is fixedly arranged on the top surface of the storage box 3, when one storage cylinder 24 needs to be taken out, firstly, the bolt 16 is rotated reversely until the rod part of the bolt 16 is screwed out by about two thirds, the adjusting button 31 is pressed, the storage cylinder 24 in the storage box 3 is driven to move by a starting motor 19, when the storage cylinder 24 is observed to be not moved through the glass 30, at the moment, one storage cylinder 24 is clamped into the taking hole 15 under the resetting action of a spring 29, the adjusting button 31 is loosened, the motor 19 stops working, then, the bolt 16 is completely screwed off, the storage cylinder 24 is taken out from the insert sleeve 23, the cover 25 on the storage cylinder 24 is taken out, the cover 25 is screwed on, the storage barrel 24 is inserted into the plug bush 23 again, the bolt 16 is screwed on, and the bolt 16 abuts against the plug bush 23 to reset.

A control system of a cell freezing and storing device for reproductive embryonic stem cells comprises a main control CPU32, a power supply module 33, an information acquisition module 34, a display module 35, a storage module 36 and an execution module 37, wherein the main control CPU32, the power supply module 33, the information acquisition module 34, the display module 35, the storage module 36 and the execution module 37 form a controller 14, the output ends of the power supply module 33 and the information acquisition module 34 are electrically connected with the input end of a main control CPU32, the input end of the power supply module 33 is electrically connected with the output end of a battery 12, the power supply module 33 transmits the electric energy of the battery 12 to the main control CPU32, the input end of the information acquisition module 34 is electrically connected with the output end of a temperature sensor 11, the information acquisition module 34 transmits the data signal of the temperature sensor 11 to the main control CPU32, the input ends of the display module 35, the storage module 36 and the, wherein memory module 36 can save the temperature data of depositing stem cell etc, touch-sensitive screen 2 is connected to master control CPU 32's input electricity, password keyboard 6 and adjustment button 31's output, display module 35's output is connected with touch-sensitive screen 2's input electricity, display module 35 can show the data that show through touch-sensitive screen 2, intelligent lock 7, the input of solenoid valve 10 and motor 19 is connected to execution module 37's output electricity, can start motor 19 through pressing adjustment button 31 and triggering execution module 37 through master control CPU32, can open or close solenoid valve 10 through operation touch-sensitive screen 2 and triggering execution module 37 through master control CPU 32.

The working principle is as follows: when the device works, a liquid nitrogen tank 8 fills liquid nitrogen into a storage box 3 through a conveying pipe 9, a battery 12 provides electric energy for the device, a temperature sensor 11 detects the temperature in the storage box 3 in real time and displays the temperature through a touch screen 2, when a cover body 4 needs to be opened, a worker must input a correct password through a password keyboard 6 to open an intelligent lock 7 so as to open the cover body 4, a regulating button 31 is pressed and is not loosened, an execution module 37 in a controller 14 is triggered, a motor 19 works, the motor 19 drives a rotating shaft 17 to rotate through gear engagement, the rotating shaft 17 drives a placing frame 22 to move through a support 21, so that a plurality of storage cylinders 24 in the storage box 3 rotate by taking the rotating shaft 17 as a center, balls 26 at the top ends of the storage cylinders 24 are attached to the inner top wall of the storage box 3 to slide, a spring 29 in the placing frame 22 is in a stretching deformation state, and the condition of stem cells in the transparent storage, when it is desired to remove one of the cartridges 24, the bolt 16 is first rotated in the reverse direction until the shank of the bolt 16 is about two thirds of the way out, when the cartridge 24 is no longer moved, as observed through the glass 30, one cartridge 24 is now blocked in the access opening 15 by the return action of the spring 29, the adjustment button 31 is released, the motor 19 stops, then the bolt 16 is completely screwed off, the guide block 27 is driven by the reset action of the spring 29 in the placing frame 22 to continuously slide upwards along the guide groove 28, the guide block 27 drives the plug bush 23 to jack the taking hole 15, at the moment, the storage barrel 24 is exposed from the taking hole 15, then, the worker withdraws the cartridge 24 from the insert 23, removes the lid 25 of the cartridge 24 to take out the stem cells, and after taking out the stem cells, the cover 25 is screwed on, the storage barrel 24 is inserted into the plug bush 23 again, the bolt 16 is screwed on, and the bolt 16 abuts against the plug bush 23 to reset.

It is noted that in the present disclosure, unless otherwise explicitly specified or limited, a first feature "on" or "under" a second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.

Claims

1. A cell cryopreservation device for reproductive embryonic stem cells, comprising a housing (1) and a storage box (3), characterized in that:

the touch screen (2) is fixedly mounted on the right side of the shell (1), the storage box (3) is fixedly mounted inside the shell (1), a cover body (4) is arranged right above the storage box (3), a liquid nitrogen tank (8) is spliced on the top of the left side of the shell (1), a conveying pipe (9) is communicated between the liquid nitrogen tank (8) and the storage box (3), an electromagnetic valve (10) is fixedly mounted on the conveying pipe (9), a temperature sensor (11) is fixedly mounted on the left side of the bottom of the storage box (3), a controller (14) is fixedly mounted at the bottom of the right side of the inside of the shell (1), a taking hole (15) is formed in the right side of the top wall of the storage box (3), a bolt (16) is fixedly mounted in the taking hole (15), and a rotating shaft (17) is movably mounted in the middle of the bottom wall of the storage, a fixed sleeve (20) is fixedly mounted at the top end of the rotating shaft (17), a plurality of brackets (21) are fixedly connected to the outer side of the fixed sleeve (20), and a placing frame (22) is fixedly mounted at one end, far away from the fixed sleeve (20), of each bracket (21);

plug bush (23) have been placed to the inside of rack (22), it has storage cylinder (24) to peg graft at the top of plug bush (23), lid (25) are installed to the bottom opening part of storage cylinder (24), the top fixed mounting of storage cylinder (24) has ball (26), the equal fixed mounting in both sides bottom of plug bush (23) has guide block (27), guide slot (28) have been seted up to the inside wall of rack (22), guide block (27) and guide slot (28) sliding connection, fixedly connected with spring (29) between the interior roof of guide block (27) and guide slot (28), the top surface embedding of depositing case (3) has glass (30), the inboard of glass (30) is provided with adjustment button (31).

2. A cell cryopreservation device for reproductive embryonic stem cells as claimed in claim 1 wherein:

lid (4) are connected with casing (1) through intelligent lock (7), fixedly connected with handle (5) in the middle of the top of lid (4), lid (4) are close to top one side fixed mounting of intelligent lock (7) have code keypad (6).

3. A cell cryopreservation device for reproductive embryonic stem cells as claimed in claim 1 wherein:

the inside left side bottom fixed mounting of casing (1) has battery (12), the left side of battery (12) is provided with interface (13) that charge.

4. A cell cryopreservation device for reproductive embryonic stem cells as claimed in claim 1 wherein:

the bottom end of the rotating shaft (17) is fixedly provided with a driven gear (18), and the driven gear (18) is meshed with a gear on the output end of the motor (19).

5. A cell cryopreservation device for reproductive embryonic stem cells as claimed in claim 1 wherein:

a plurality of said stents (21) are arranged in a circumferential array;

the placing rack (22) is a U-shaped rack.

6. A cell cryopreservation device for reproductive embryonic stem cells as claimed in claim 1 wherein:

the plug bush (23) is cylindrical;

the diameter value of the top end of the storage cylinder (24), the external diameter value of the plug bush (23) and the internal diameter value of the taking hole (15) are all equal.

7. A cell cryopreservation device for reproductive embryonic stem cells as claimed in claim 1 wherein:

the guide block (27) is matched with the guide groove (28);

the adjusting button (31) is fixedly arranged on the top surface of the storage box (3).