Quantum dot fluorescence detection device and quantum dot fluorescence monitor
Technical Field
The utility model relates to a technical field is used in biological science research and medical science inspection, particularly, relates to a quantum dot fluorescence detection device and quantum dot fluorescence monitor.
Background
The physical, optical and electrical characteristics of the quantum dots are far superior to those of the existing organic fluorescent dyes, the quantum dots have the advantages of high sensitivity, good stability, long validity period and the like, and the quantum dots can be widely applied to the technical fields of high sensitivity, living body/in-vivo long-time dynamic observation, multi-index simultaneous detection and the like as a marking probe.
In clinical practice, quantum dot labeled probes are usually disposed on a test strip, and the test strip generally includes a control line and a detection line, namely a C line and a T line.
In a conventional quantum dot assay device and method, an ultraviolet light source or visible light is generally used to irradiate a test strip, and fluorescence generated on the test strip is obtained together by an image pickup device or a photoelectric sensing device to perform analysis and assay. However, since the C line and the T line on the test strip have a certain distance, the distances between the light source (or the image pickup device and the photoelectric sensing device) and the C line and the T line are different, and the outgoing angle and the incident angle of the light are also different. In the sample measuring process, the light emitted from a specific fixed position is used to irradiate the T line and the C line, and the fluorescence data on the T line and the C line is collected from a specific fixed position, so that the accuracy of the experimental result is difficult to guarantee.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a quantum dot fluorescence detection device, its detection position that can adjust detection module according to the position relation of T line and C line on the test paper strip to the realization is to the accurate survey of analyte on the test paper strip.
Another object of the utility model is to provide a quantum dot fluorescence monitor, it has the effect of accurate fluorescence data on collecting T line and the C line in order to improve monitoring, contrast and analysis.
The embodiment of the utility model is realized like this:
a quantum dot fluorescence detection device comprises a base, a guide column and a detection module; the guide post is erected on the base, the detection module is movably arranged on the guide post, and the detection module can move along the length direction of the guide post relative to the base; the detection module comprises an emergent light source and a fluorescent probe; the emergent light source is used for irradiating the test strip, and the fluorescent probe is used for collecting fluorescence generated on the test strip.
By adopting the quantum dot fluorescence detection device, when a test strip is detected according to the position relation of the T line and the C line on the test strip, the lifting movement of the detection module on the guide column is controlled, the T line and the C line are sequentially irradiated at the same distance and angle, and the fluorescence information on the T line and the C line is respectively collected for analysis and determination, so that the detection and contrastive analysis accuracy is improved.
In a preferred embodiment of the present invention, the device further comprises a stepping motor; the stepping motor is arranged on the base and used for driving the detection module to move along the guide post. The technical effects are as follows: because the distance of T line and C line all is invariable numerical value on the test paper strip generally, utilize step motor to go up and down the detection module according to specific distance, improved the positioning speed and the experimental efficiency of detection module.
In a preferred embodiment of the present invention, the stepping motor is provided with a lifting column, the guiding column is provided with a guiding hole, and the lifting column passes through the guiding hole; the detection module is arranged on the lifting column, and the stepping motor is used for driving the lifting column to move along the axis direction of the guide hole. The technical effects are as follows: the cooperation of guiding hole and lift post can prevent to survey the skew of module emergence position in frequent activity, has guaranteed the accurate positioning of surveying the module.
In a preferred embodiment of the present invention, the exit light source includes an LED light source. The technical effects are as follows: the LED light source has the advantages of small heat productivity, low energy consumption, small installation space and long service life, and preferably, the LED lamp beads are arranged inside the integrated structure of the detection module, and light rays are transmitted out through a narrow strip-shaped through hole so as to accurately irradiate the test strip.
In a preferred embodiment of the present invention, the fluorescence probe comprises a photodiode detector. The technical effects are as follows: different from the traditional photographing imaging scheme, the photodiode detector can accurately and rapidly reflect the light intensity of fluorescence, and meanwhile, the photodiode detector is compact in structure and small in installation space. It should be noted that the LED light source and the photodiode detector should be sequentially and fixedly mounted in the length direction of the test strip, the light emitting direction of the LED light source and the direction of the photodiode detector form a certain angle and intersect with each other, and the intersection point position can directly correspond to the T line or the C line of the test strip.
A quantum dot fluorescence monitor comprises a shell, a test paper placing piece and the quantum dot fluorescence detection device, wherein the test paper placing piece and a base are both arranged in the shell; the test strip is placed in the test strip placing piece, and the length direction of the test strip is parallel to the moving direction of the detection module; the detection module is driven to move, the light of the emergent light source can sequentially irradiate the T line and the C line on the test strip, and the fluorescent probe can sequentially collect the fluorescence emitted by the T line and the fluorescence emitted by the C line on the test strip.
In a preferred embodiment of the present invention, the test paper placing member includes a rotating base and a rotating cylinder disposed on the rotating base; the rotating seat is arranged in the inner cavity of the shell and used for driving the rotating cylinder to rotate; a plurality of test strips can be placed on the rotary drum. The technical effects are as follows: because a plurality of test strips can be placed in the rotary drum, and the rotary drum is driven by the rotary seat to rotate, the T line and the C line of one test strip can be measured immediately, and then the other test strip can be measured. Therefore, in a measuring period, the quantum dot fluorescence monitor can detect a plurality of test strips, and the monitoring efficiency of the experiment is greatly improved.
In the preferred embodiment of the present invention, the utility model further comprises a reset device; the resetting device is arranged at the bottom of the rotating seat. The technical effects are as follows: after all the test paper strips in one rotary drum are tested, the rotary base can be restored to the initial position through the resetting device, so that the initial position of the next rotary drum can be conveniently determined.
In a preferred embodiment of the present invention, a support cushion is disposed at the bottom of the housing. The technical effects are as follows: in the experimental process, the detection module frequently goes up and down to step motor, and in order to keep the stability of equipment, the support cushion can play the effect of buffering, reduces the negative influence that vibrations brought.
In a preferred embodiment of the present invention, the device further comprises a flip cover, wherein the flip cover is disposed on the housing. The technical effects are as follows: the flip cover is arranged at the position of the shell corresponding to the rotating seat, and the rotating cylinder can be replaced by opening the flip cover, so that the convenience and the rapidness are realized.
The embodiment of the utility model provides a beneficial effect is:
the utility model discloses a quantum dot fluorescence detection device sets up emergent light source and fluorescence probe integration into the detection module, can adjust the detection position of detection module according to the position relation of T line and C line on the test paper strip, and the oscilaltion of control detection module can acquire the fluorescence that T line and C line produced on the test paper strip respectively, through calculation and comparison to fluorescence intensity, the analyte of being examined on the accurate determination test paper strip.
The utility model discloses a quantum dot fluorescence monitor when having above-mentioned quantum dot fluorescence detection device's advantage, places the test paper strip part and detection device and places in the shell in concentrating, forms a complete and confined monitoring mechanism, has effectively promoted the reliability and the independence of experiment.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention, and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
Fig. 1 is a schematic perspective view of a quantum dot fluorescence detection device according to a first embodiment of the present invention;
fig. 2 is a front view of a quantum dot fluorescence detection device according to a first embodiment of the present invention;
fig. 3 is a right side view of a quantum dot fluorescence detection device according to a first embodiment of the present invention;
fig. 4 is a schematic view of an internal structure of a quantum dot fluorescence monitor (with a rotary base and a rotary drum removed) according to a second embodiment of the present invention;
fig. 5 is a schematic view of an internal structure of a quantum dot fluorescence monitor according to a second embodiment of the present invention;
fig. 6 is a schematic view of an external structure of a quantum dot fluorescence monitor according to a second embodiment of the present invention;
fig. 7 is a front view of a quantum dot fluorescence monitor according to a second embodiment of the present invention.
In the figure: 1-a base; 2-a guide post; 3-emitting a light source; 4-a fluorescent probe; 5-a step motor; 6-lifting column; 7-a guide hole; 8-a housing; 9-a rotating seat; 10-a rotating drum; 11-a reset device; 12-a support cushion; 13-flip cover.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of the embodiments of the present invention, as generally described and illustrated in the figures herein, can be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate the position or positional relationship based on the position or positional relationship shown in the drawings, or the position or positional relationship which is usually placed when the product of the present invention is used, and are only for convenience of description and simplification of the description, but do not indicate or imply that the device or element referred to must have a specific position, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance.
Furthermore, the terms "horizontal", "vertical", "overhang" and the like do not imply that the components are required to be absolutely horizontal or overhang, but may be slightly inclined. For example, "horizontal" merely means that the direction is more horizontal than "vertical" and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.
In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Some embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The embodiments described below and the features of the embodiments can be combined with each other without conflict.
The first embodiment:
fig. 1 is a schematic perspective view of a quantum dot fluorescence detection device according to a first embodiment of the present invention; fig. 2 is a front view of a quantum dot fluorescence detection device according to a first embodiment of the present invention; fig. 3 is a right side view of the quantum dot fluorescence detection device according to the first embodiment of the present invention.
Referring to fig. 1 to 3, the present embodiment provides a quantum dot fluorescence detection device, which includes a base 1, a guide post 2, and a detection module; the guide post 2 is erected on the base 1, the detection module is movably arranged on the guide post 2, and the detection module can move along the length direction of the guide post 2 relative to the base 1; the detection module comprises an emergent light source 3 and a fluorescent probe 4; the light emitting direction of the emergent light source 3 and the straight line where the fluorescent probe 4 is located can be intersected at a focus, the emergent light source 3 is used for irradiating the test strip, and the fluorescent probe 4 is used for collecting fluorescence generated on the test strip.
As shown in fig. 1, 2 and 3, the device further comprises a stepping motor 5; the stepping motor 5 is arranged on the base 1 and used for driving the detection module to move along the guide post 2.
Optionally, the lifting driving device of the detection module may also selectively use a worm and gear structure, after the worm gear rotates by a certain angle, the worm may move by a certain distance to realize lifting, and after the worm gear rotates by a certain angle in the opposite direction, the worm may move by a certain distance to realize lowering.
As shown in fig. 1, 2 and 3, the stepping motor 5 is provided with a lifting column 6, the guide column 2 is provided with a guide hole 7, and the lifting column 6 passes through the guide hole 7; the detection module is arranged on the lifting column 6, and the stepping motor 5 is used for driving the lifting column 6 to move along the axial direction of the guide hole 7.
Alternatively, the combination structure of the guide hole 7 and the lifting column 6 may be replaced by a combination structure of a guide rail and a guide groove, the guide groove is arranged on the guide column 2, the guide rail is arranged on the detection module, and the detection module is guided by the guide column 2 to move back and forth in a specific direction.
As shown in fig. 1 and 3, the light source 3 includes an LED light source. Preferably, LED lamp pearl is preferred to be set up inside the integrated configuration of detecting the module to light is passed through the bar through-hole of a narrow and small, with the accurate test paper strip that shines. The illumination range formed by the shape of the strip-shaped through hole corresponds to the T line and the C line of the test strip.
As shown in fig. 1 and 3, the fluorescence probe 4 includes a photodiode detector.
Preferably, the LED light source and the photodiode detector should be sequentially and fixedly mounted in the length direction of the test strip, the light emitting direction of the LED light source and the direction of the photodiode detector form a certain angle and intersect, and the intersection point position can directly correspond to the T line or the C line of the test strip.
The working principle of the quantum dot fluorescence detection device is as follows: firstly, the emergent light source 3 irradiates the T line position (or the C line position) of the test strip, a sample to be detected on the test strip generates fluorescence after being irradiated, and the fluorescence probe 4 immediately collects the fluorescence signal and carries out processing analysis. Then, the detection module changes the height position thereof, so that the emergent light source 3 irradiates the C line position (or the T line position) of the test strip, the sample to be detected on the test strip generates fluorescence after being irradiated, and the fluorescence probe 4 immediately collects the fluorescence signal and carries out processing analysis. And the detected analyte on the test strip is accurately measured by calculating and comparing the fluorescence intensity. In the process, the quantum dot fluorescence detection device controls the lifting movement of the detection module on the guide post 2 when detecting a test strip according to the position relation of the T line and the C line on the test strip, sequentially irradiates the T line and the C line at the same distance and angle and respectively collects fluorescence information on the T line and the C line for analysis and determination, and improves the accuracy of detection and contrastive analysis.
Second embodiment:
fig. 4 is a schematic view of an internal structure of a quantum dot fluorescence monitor (with the rotary base 9 and the rotary drum 10 removed) according to a second embodiment of the present invention; fig. 5 is a schematic view of an internal structure of a quantum dot fluorescence monitor according to a second embodiment of the present invention; fig. 6 is a schematic view of an external structure of a quantum dot fluorescence monitor according to a second embodiment of the present invention; fig. 7 is a front view of a quantum dot fluorescence monitor according to a second embodiment of the present invention. Referring to fig. 4 to 7, the present embodiment provides a quantum dot fluorescence monitor, which includes a housing 8, a test paper placing member, and the quantum dot fluorescence detecting device, wherein the test paper placing member and the base 1 are both disposed inside the housing 8; the test strip is placed in the test strip placing piece, and the length direction of the test strip is parallel to the moving direction of the detection module; the detection module is driven to move, the light of the emergent light source 3 can sequentially irradiate a T line and a C line on the test strip, and the fluorescent probe 4 can sequentially collect fluorescence emitted by the T line and fluorescence emitted by the C line on the test strip.
As shown in fig. 4 and 5, the test paper placing member includes a rotating base 9 and a rotating cylinder 10 disposed on the rotating base 9; the rotating seat 9 is arranged in the inner cavity of the shell 8 and used for driving the rotating cylinder 10 to rotate; a plurality of test strips can be placed on the rotary drum 10.
As shown in fig. 4 and 5, the device further includes a reset device 11; the resetting device 11 is arranged at the bottom of the rotary seat 9. Optionally, the resetting device 11 may be a couple-type rotational positioning device, or a magnetic directional returning device.
Wherein a support cushion 12 is provided at the bottom of the housing 8, as shown in fig. 7. The number of the supporting cushions 12 is set to be 3, 4, 5 or 6, etc., and is uniformly distributed on the bottom surface of the housing 8.
As shown in fig. 6, the portable electronic device further includes a flip 13, and the flip 13 is disposed on the housing 8. Optionally, the flip cover 13 may also be selectively used with a pull-out cover to reduce the operating space of the device.
The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.