CN210742131U - Quantum dot fluorescence detection device and quantum dot fluorescence monitor - Google Patents

Abstract

The utility model provides a quantum dot fluorescence detection device, which relates to the technical field of biological scientific research and medical examination application. The quantum dot fluorescence detection device includes a base, a guide column and a detection module; the guide column is erected on the base, the detection module is movably arranged on the guide column, and the detection module can move relative to the base along the length direction of the guide column; the detection module It includes an outgoing light source and a fluorescence probe; the outgoing light source is used to illuminate the test strip, and the fluorescence probe is used to collect the fluorescence generated on the test strip. The quantum dot fluorescence detection device of the utility model integrates the outgoing light source and the fluorescence probe into a detection module, and the fluorescence generated by the T line and the C line on the test strip can be obtained respectively by controlling the up and down of the detection module, and by calculating the fluorescence intensity And comparison, accurate determination of the analyte on the test strip. On this basis, the utility model also provides a quantum dot fluorescence monitor.

Description

Quantum dot fluorescence detection device and quantum dot fluorescence monitor

technical field

The utility model relates to the technical field of biological scientific research and medical inspection application, in particular to a quantum dot fluorescence detection device and a quantum dot fluorescence monitor.

Background technique

The physical, optical, and electrical properties of quantum dots are far superior to those of existing organic fluorescent dyes, and they have the advantages of high sensitivity, good stability, and long validity period. Multi-index simultaneous detection and other technical fields.

In clinical practice, quantum dot-labeled probes are usually set on test strips, and test strips generally include control lines and detection lines, which are often referred to as C lines and T lines.

Conventional quantum dot measurement equipment and methods usually use ultraviolet light source or visible light to irradiate the test strip, and then acquire the fluorescence generated on the test strip through imaging equipment or photoelectric sensing equipment for analysis and measurement. However, because there is a certain distance between the C line and the T line on the test strip, the distance between the light source (or camera equipment, photoelectric sensing equipment) and the C line and the T line must be different, and the exit and incidence angles of the light are also different. same. In the process of sample measurement, it is difficult to guarantee the accuracy of the experimental results if the light emitted from a specific fixed position is generally used to irradiate the T line and the C line, and a specific fixed position is used to collect the fluorescence data on the T line and the C line.

Utility model content

One object of the present invention is to provide a quantum dot fluorescence detection device, which can adjust the detection position of the detection module according to the positional relationship between the T line and the C line on the test strip, so as to realize the accurate detection of the analyte on the test strip. Determination.

Another object of the present invention is to provide a quantum dot fluorescence monitor, which has the effect of accurately collecting fluorescence data on T-line and C-line to improve monitoring, comparison and analysis.

The embodiment of the present utility model is realized in this way:

A quantum dot fluorescence detection device, comprising a base, a guide column and a detection module; the guide column is erected on the base, the detection module is movably arranged on the guide column, and the detection module can be relatively The base moves along the length direction of the guide column; the detection module includes an outgoing light source and a fluorescence probe; the outgoing light source is used to illuminate the test strip, and the fluorescent probe is used to collect the light generated on the test strip. Fluorescence.

Using the above quantum dot fluorescence detection device, according to the positional relationship between the T line and the C line on the test strip, when a test strip is detected, the lifting activity of the detection module on the guide column is controlled, and the same distance and angle are followed in sequence. Irradiate T line and C line and collect fluorescence information on T line and C line respectively for analysis and determination, which improves the accuracy of detection and comparative analysis.

In a preferred embodiment of the present invention, a stepping motor is further included; the stepping motor is arranged on the base and is used to drive the detection module to move along the guide column. The technical effect is: since the distance between the T line and the C line on the test strip is generally a constant value, the stepper motor can be used to move the detection module up and down according to a specific distance, which improves the positioning speed and experimental efficiency of the detection module.

In a preferred embodiment of the present invention, the stepping motor is provided with a lifting column, the guide column is provided with a guide hole, and the lifting column passes through the guide hole; the detection module is arranged on the lifting column , the stepping motor is used to drive the lifting column to move along the axis direction of the guide hole. The technical effect is that the cooperation between the guide hole and the lifting column can prevent the position deviation of the detection module during frequent activities, and ensure the accurate positioning of the detection module.

In a preferred embodiment of the present invention, the outgoing light source includes an LED light source. The technical effect is that the LED light source has low heat generation, low energy consumption, small installation space, and long service life. The holes let light through to accurately illuminate the test strip.

In a preferred embodiment of the present invention, the fluorescent probe includes a photodiode detector. The technical effect is: different from the traditional photographing and imaging scheme, the photodiode detector can accurately and quickly reflect the light intensity of the fluorescence, and at the same time, the photodiode detector has a compact structure and a small installation space. It should be noted that the above-mentioned LED light source and photodiode detector should be fixed and installed in sequence according to the length direction of the test strip, and the light emission direction of the LED light source and the direction of the photodiode detector form a certain angle and intersect. The position of the intersection point can directly correspond to the T line or C line of the test strip.

A quantum dot fluorescence monitor, comprising a casing, a test paper placement piece and the above-mentioned quantum dot fluorescence detection device, wherein the test paper placement piece and the base are all arranged inside the casing; a test strip is placed on the test paper In the placement piece, the length direction of the test strip is parallel to the moving direction of the detection module; the detection module is driven to move, and the light from the outgoing light source can sequentially illuminate the T line and the C line on the test strip. The fluorescence probe can sequentially collect the fluorescence emitted by the T line and the fluorescence emitted by the C line on the test strip.

In a preferred embodiment of the present invention, the test paper placing member includes a rotating base and a rotating cylinder arranged on the rotating base; the rotating base is disposed in the inner cavity of the outer casing and is used to drive the rotation The cylinder rotates; a plurality of test strips can be placed on the rotating cylinder. The technical effect is: since a plurality of test strips can be placed in the rotating cylinder, and the rotating cylinder is driven to rotate by the rotating base, after the T line and C line of one test strip are measured, the measurement of another test strip can be started immediately. . Therefore, in one measurement cycle, the quantum dot fluorescence monitor can detect multiple test strips, which greatly improves the monitoring efficiency of the experiment.

In a preferred embodiment of the present invention, a reset device is also included; the reset device is arranged on the bottom of the rotating seat. The technical effect is that after all the test strips in one rotating cylinder are measured, the rotating base can be restored to the initial position through the reset device, so as to facilitate the determination of the initial position of the next rotating cylinder.

In a preferred embodiment of the present invention, the bottom of the casing is provided with a support cushion. The technical effect is that during the experiment, the stepper motor frequently lifts and lowers the detection module. In order to maintain the stability of the equipment, the support cushion can play a buffering role and reduce the negative impact of vibration.

In a preferred embodiment of the present invention, it further includes a flip cover, and the flip cover is arranged on the casing. The technical effect is that the flip cover is arranged at the position of the casing corresponding to the rotating seat, and the rotating cylinder can be replaced by opening the flip cover, which is convenient and quick.

The beneficial effects of the embodiment of the present utility model are:

The quantum dot fluorescence detection device of the utility model integrates the outgoing light source and the fluorescence probe into a detection module, which can adjust the detection position of the detection module according to the positional relationship between the T line and the C line on the test strip, and control the up and down movement of the detection module. The fluorescence generated by the T line and the C line on the test strip can be obtained separately, and the analyte to be detected on the test strip can be accurately determined by calculating and comparing the fluorescence intensity.

The quantum dot fluorescence monitor of the utility model has the advantages of the above-mentioned quantum dot fluorescence detection device, and at the same time, the test strip placement parts and the detection device are built into the casing, forming a complete and closed monitoring mechanism, which effectively improves the Experiment reliability and independence.

Description of drawings

In order to illustrate the technical solutions of the embodiments of the present invention more clearly, the accompanying drawings that need to be used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present invention. Therefore, it should not be regarded as a limitation of the scope. For those of ordinary skill in the art, other related drawings can also be obtained from these drawings without any creative effort.

1 is a schematic three-dimensional structure diagram of a quantum dot fluorescence detection device provided by the first embodiment of the present invention;

2 is a front view of the quantum dot fluorescence detection device provided by the first embodiment of the present invention;

3 is a right side view of the quantum dot fluorescence detection device provided by the first embodiment of the present invention;

4 is a schematic diagram of the internal structure of the quantum dot fluorescence monitor (with the rotating base and the rotating cylinder removed) provided by the second embodiment of the present invention;

5 is a schematic diagram of the internal structure of the quantum dot fluorescence monitor provided by the second embodiment of the present invention;

6 is a schematic diagram of the external structure of the quantum dot fluorescence monitor provided by the second embodiment of the present invention;

FIG. 7 is a front view of the quantum dot fluorescence monitor provided by the second embodiment of the present invention.

In the figure: 1-base; 2-guide column; 3-outgoing light source; 4-fluorescence probe; 5-stepper motor; 6-lifting column; 7-guide hole; 8-shell; 9-rotating seat; 10- Rotary cylinder; 11-reset device; 12-support cushion; 13-flip cover.

Detailed ways

In order to make the purposes, technical solutions and advantages of the embodiments of the present utility model clearer, the technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model. The embodiments described above are a part of the embodiments of the present invention, but not all of the embodiments. The components of the embodiments of the invention generally described and illustrated in the drawings herein can be arranged and designed in a variety of different configurations.

Accordingly, the following detailed description of the embodiments of the invention provided in the accompanying drawings is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present invention.

It should be noted that like numerals and letters refer to like items in the following figures, so once an item is defined in one figure, it does not require further definition and explanation in subsequent figures.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner" and "outer" The orientation or positional relationship indicated by etc. is based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship that is usually placed when the utility model product is used, only for the convenience of describing the present utility model and simplifying the description, not Indicates or implies that the referred device or element must have a particular orientation, be constructed and operate in a particular orientation, and therefore should not be construed as a limitation of the present invention. Furthermore, the terms "first", "second", "third", etc. are only used to differentiate the description and should not be construed as indicating or implying relative importance.

Furthermore, the terms "horizontal", "vertical", "overhanging" etc. do not imply that a component is required to be absolutely horizontal or overhang, but rather may be slightly inclined. For example, "horizontal" only means that its direction is more horizontal than "vertical", it does not mean that the structure must be completely horizontal, but can be slightly inclined.

In the description of the present invention, it should also be noted that, unless otherwise expressly specified and limited, the terms "arrangement", "installation", "connection" and "connection" should be understood in a broad sense, for example, it may be a fixed connection It can also be a detachable connection or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or an indirect connection through an intermediate medium, or the internal communication between the two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood in specific situations.

Some embodiments of the present utility model will be described in detail below with reference to the accompanying drawings. The embodiments described below and features in the embodiments may be combined with each other without conflict.

First embodiment:

1 is a schematic three-dimensional structure diagram of the quantum dot fluorescence detection device provided by the first embodiment of the present invention; FIG. 2 is a front view of the quantum dot fluorescence detection device provided by the first embodiment of the present invention; FIG. 3 is the first embodiment of the present invention. The right side view of the quantum dot fluorescence detection device provided in an embodiment.

Referring to FIGS. 1 to 3 , the present embodiment provides a quantum dot fluorescence detection device, which includes a base 1 , a guide column 2 and a detection module; the guide column 2 is erected on the base 1 , and the detection module is movably arranged on the guide column 2 The detection module can move relative to the base 1 along the length direction of the guide column 2; the detection module includes the outgoing light source 3 and the fluorescence probe 4; the light emitting direction of the outgoing light source 3 and the direction of the fluorescence probe 4 One focus, the outgoing light source 3 is used to illuminate the test strip, and the fluorescence probe 4 is used to collect the fluorescence generated on the test strip.

Wherein, as shown in FIG. 1 , FIG. 2 , and FIG. 3 , a stepping motor 5 is further included; the stepping motor 5 is arranged on the base 1 and is used to drive the detection module to move along the guide column 2 .

Optionally, the lift drive device of the detection module can also choose to use a turbine worm structure. After the turbine rotates at a certain angle, the worm can move a certain distance to achieve elevation. After the turbine rotates in the opposite direction for a certain angle, the worm can move a certain amount. The distance achieved is decreased.

Among them, as shown in Figure 1, Figure 2, Figure 3, the stepping motor 5 is provided with a lifting column 6, the guide column 2 is provided with a guide hole 7, and the lifting column 6 passes through the guide hole 7; the detection module is arranged on the lifting column 6, and the step The feeding motor 5 is used to drive the lifting column 6 to move along the axis direction of the guide hole 7 .

Optionally, the combined structure of the guide hole 7 and the lifting column 6 can also be replaced by a combined structure of a guide rail and a guide groove, a guide groove is set on the guide column 2 and a guide rail is set on the detection module, and the guide column 2 is used to guide the detection module according to Move back and forth in a specific direction.

Wherein, as shown in FIG. 1 and FIG. 3 , the outgoing light source 3 includes an LED light source. Preferably, the LED lamp bead is preferably arranged inside the integrated structure of the detection module, and transmits light through a narrow strip-shaped through hole, so as to accurately illuminate the test strip. The illumination range formed by the shape of the strip-shaped through hole corresponds to the T line and the C line of the test strip.

Among them, as shown in FIG. 1 and FIG. 3 , the fluorescence probe 4 includes a photodiode detector.

Preferably, the above-mentioned LED light source and photodiode detector should be fixed and installed in sequence according to the length direction of the test strip, and the light emitting direction of the LED light source and the direction of the photodiode detector form a certain angle and intersect, and the position of the intersection point It can directly correspond to the T line or C line of the test strip.

The working principle of the quantum dot fluorescence detection device is as follows: first, the outgoing light source 3 illuminates the T line position (or C line position) of the test strip, and the sample to be tested on the test strip generates fluorescence after being irradiated, and the fluorescence probe 4 collects it immediately. The fluorescent signal is processed and analyzed. Then, the detection module changes its height position, so that the outgoing light source 3 illuminates the C-line position (or T-line position) of the test strip, the sample to be tested on the test strip generates fluorescence after being irradiated, and the fluorescence probe 4 collects the fluorescence immediately signal and analyze it. Through the calculation and comparison of the fluorescence intensity, the detected analyte on the test strip can be accurately determined. In this process, according to the positional relationship between the T line and the C line on the test strip, the quantum dot fluorescence detection device controls the lifting and lowering activities of the detection module on the guide column 2 when detecting a test strip, at the same distance The T line and the C line are irradiated in turn and the fluorescence information on the T line and the C line are respectively collected for analysis and determination, which improves the accuracy of detection and comparative analysis.

Second embodiment:

FIG. 4 is a schematic diagram of the internal structure of the quantum dot fluorescence monitor provided by the second embodiment of the present invention (with the rotating base 9 and the rotating drum 10 removed); FIG. 5 is the quantum dot fluorescence monitor provided by the second embodiment of the present invention. Figure 6 is a schematic diagram of the external structure of the quantum dot fluorescence monitor provided by the second embodiment of the present invention; Figure 7 is a front view of the quantum dot fluorescence monitor provided by the second embodiment of the present invention. Referring to FIGS. 4 to 7 , the present embodiment provides a quantum dot fluorescence monitor, which includes a housing 8 , a test paper placement member, and the above-mentioned quantum dot fluorescence detection device. The test paper placement member and the base 1 are all disposed inside the housing 8 ; The test strip is placed in the test strip holder, and the length direction of the test strip is parallel to the moving direction of the detection module; the detection module is driven to move, and the light emitted from the light source 3 can illuminate the T line and C line on the test strip in turn, The fluorescence probe 4 can sequentially collect the fluorescence emitted by the T line and the fluorescence emitted by the C line on the test strip.

Among them, as shown in Figures 4 and 5, the test paper placement member includes a rotating base 9 and a rotating cylinder 10 arranged on the rotating base 9; A plurality of test strips can be placed on the cartridge 10 .

Wherein, as shown in FIG. 4 and FIG. 5 , a reset device 11 is also included; the reset device 11 is arranged on the bottom of the rotating base 9 . Optionally, the reset device 11 can be a galvanic-type rotary positioning device, or a magnetic directional reset device.

Wherein, as shown in FIG. 7 , the bottom of the casing 8 is provided with a support cushion 12 . The number of supporting soft pads 12 should be set to 3, 4, 5 or 6, etc., and are evenly distributed on the bottom surface of the casing 8 .

Wherein, as shown in FIG. 6 , a flip cover 13 is also included, and the flip cover 13 is arranged on the casing 8 . Optionally, the flip cover 13 can also choose to use a pull-out cover to reduce the operating space of the device.

The above are only preferred embodiments of the present invention, and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims (10)

1. A quantum dot fluorescence detection device, characterized in that, comprising a base (1), a guide post (2) and a detection module; The guide column (2) is erected on the base (1), the detection module is movably arranged on the guide column (2), and the detection module can be relative to the base (1) along the the length direction of the guide column (2) moves; The detection module includes an outgoing light source (3) and a fluorescence probe (4); the outgoing light source (3) is used to illuminate the test strip, and the fluorescence probe (4) is used to collect fluorescence generated on the test strip. 2 . The quantum dot fluorescence detection device according to claim 1 , further comprising a stepping motor ( 5 ); the stepping motor ( 5 ) is arranged on the base ( 1 ) for driving The detection module moves along the guide column (2). 3 . The quantum dot fluorescence detection device according to claim 2 , wherein the stepping motor ( 5 ) is provided with a lifting column ( 6 ), the guide column ( 2 ) is provided with a guide hole ( 7 ), and the The lifting column (6) passes through the guide hole (7); the detection module is arranged on the lifting column (6), and the stepping motor (5) is used to drive the lifting column (6) along the The guide hole (7) moves in the axial direction. 4. The quantum dot fluorescence detection device according to any one of claims 1 to 3, wherein the outgoing light source (3) comprises an LED light source. 5. The quantum dot fluorescence detection device according to any one of claims 1 to 3, wherein the fluorescence probe (4) comprises a photodiode detector. 6. A quantum dot fluorescence monitor, characterized in that it comprises a casing (8), a test paper placement member and the quantum dot fluorescence detection device according to any one of claims 1 to 5, the test paper placement member, the test paper placement The bases (1) are all arranged inside the casing (8); the test strips are placed in the test paper placement pieces, and the length direction of the test strips is parallel to the movement direction of the detection module; The detection module is driven to move, the light from the outgoing light source (3) can be sequentially irradiated on the T line and the C line on the test strip, and the fluorescent probe (4) can sequentially collect the light emitted by the T line on the test strip. Fluorescence and fluorescence emitted by the C-line. 7 . The quantum dot fluorescence monitor according to claim 6 , wherein the test paper placement member comprises a rotating base (9) and a rotating cylinder (10) arranged on the rotating base (9); the The rotating seat (9) is arranged in the inner cavity of the casing (8), and is used for driving the rotating cylinder (10) to rotate; a plurality of test strips can be placed on the rotating cylinder (10). 8 . The quantum dot fluorescence monitor according to claim 7 , further comprising a reset device ( 11 ); the reset device ( 11 ) is arranged on the bottom of the rotating seat ( 9 ). 9 . 9 . The quantum dot fluorescence monitor according to claim 6 , characterized in that a support cushion ( 12 ) is provided at the bottom of the casing ( 8 ). 10 . 10 . The quantum dot fluorescence monitor according to claim 6 , further comprising a flip cover ( 13 ), the flip cover ( 13 ) being arranged on the housing ( 8 ). 11 .

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