CN210736727U - Plasma anaerobic microorganism breeding device - Google Patents
Plasma anaerobic microorganism breeding device Download PDFInfo
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- CN210736727U CN210736727U CN201920892676.4U CN201920892676U CN210736727U CN 210736727 U CN210736727 U CN 210736727U CN 201920892676 U CN201920892676 U CN 201920892676U CN 210736727 U CN210736727 U CN 210736727U
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Abstract
The utility model relates to a plasma anaerobic microorganism breeding device, including anaerobic microorganism operation processing module, detection module, control module, anaerobic microorganism operation processing module comprises the control chamber and through rather than the sample chamber that links to each other through the pass-gate. The utility model realizes the function of mutation treatment and culture of anaerobic bacteria.
Description
Technical Field
The utility model belongs to the technical field of plasma, in particular to plasma anaerobic microorganism breeding device.
Background
Anaerobic microorganisms exist widely in nature, which are not classified in environments where humans depend for survival and life, but the breeding work of anaerobic microorganisms is limited because the anaerobic microorganisms inhibit growth and even have toxic action in the presence of oxygen. How to utilize efficient microorganism mutagenesis technology to realize rapid optimization of strains is important work of microorganism breeding work.
The plasma is a substance fourth state coexisting with the solid, liquid and gas states of the substance, is a substance form mainly composed of a large number of free electrons and charged ions, and is electrically neutral macroscopically. Research shows that active particles existing in a large amount in the plasma can act on different biological samples, and cause improvement of various target properties, so that the method has a good application prospect in the field of biotechnology. Plasma technology has been developed rapidly in recent years as a new mutation breeding technology, and has been applied to mutation breeding of more than 100 kinds of microorganisms including bacteria, actinomycetes, fungi, yeasts, microalgae and the like, as a normal-pressure room-temperature plasma mutation breeding technology with industrial representativeness.
Although the plasma technology has been widely used for microorganisms, it is basically aerobic or facultative anaerobic microorganisms, and if the anaerobic microorganisms are subjected to plasma mutagenesis treatment, the plasma mutagenesis environment is an important factor. For the anaerobic treatment environment, application publication No. CN204848891U discloses a plasma breeding device for mutagenizing anaerobic microorganisms, which comprises a plasma generator, a sample carrying platform, a sampling chamber and an operating chamber, wherein the plasma generator and the sample carrying platform are positioned in the operating chamber, the plasma generator is over against the sample carrying platform, and the plasma generator is connected with a plasma control system at the upper part of the operating chamber; the sampling chamber is positioned outside the operating chamber, a transfer door is arranged between the sampling chamber and the operating chamber, and the sampling chamber and the operating chamber are connected with a vacuum pump at the upper part of the operating chamber; an operation window is arranged on the wall of the operation chamber, and operation gloves are arranged on the operation window. The patent uses nitrogen filling to realize anaerobic environment, and although the method meets certain anaerobic conditions, the anaerobic environment is very harsh in the application of industrial bacteria, and the level of the anaerobic environment required by the anaerobic industrial bacteria is difficult to reach.
Disclosure of Invention
In order to solve the problem, the utility model provides a plasma anaerobic microorganism breeding device both can provide strict anaerobic environment promptly oxygen content can not exceed 0.03%, can realize mutagenesis, cultivation integration operation again.
The utility model discloses technical scheme as follows:
a plasma anaerobic microorganism breeding device comprises an anaerobic microorganism operation processing module and a detection and control module,
the anaerobic microorganism operation treatment module comprises: an operation chamber and a sampling chamber connected with the operation chamber through a transfer door,
the operating room is internally provided with a plasma generator, a sample carrying table, an operating room visual show window, a culture system, a gas control system I and a gas catalysis module, wherein the plasma generator is opposite to the sample carrying table and is connected with the plasma control system on the upper part of the operating room, the plasma generator is connected with a working gas source and a radio frequency power supply module, the operating room visual show window is made of transparent materials, an operating window is arranged below the operating window, the operating window is provided with sealed operating gloves, the culture system can realize a constant-temperature culture device, the gas control system I comprises a nitrogen control valve, a vacuumizing control valve and a mixed gas control valve, and the gas catalysis module is a functional module which is arranged in the operating room and can eliminate residual oxygen and gas generated by microbial metabolism;
the sampling chamber is provided with a sampling chamber door, and a gas control system II which comprises a nitrogen control valve and a vacuumizing control valve is arranged in the sampling chamber;
the detection and control module comprises a gas flow controller for controlling a working gas source, a gas controller for controlling a gas control system I and a gas control system II, a power sensor for detecting the power of the radio frequency source power supply module, oxygen sensors respectively arranged in the operation chamber and the sampling chamber and used for detecting the oxygen environment, a temperature sensor for detecting the temperature of the culture system, an operation panel for setting parameters, and a controller, the input end of the controller is connected with an operation panel, a gas flow controller, a gas controller, a radio frequency source power supply module power sensor, an oxygen sensor and a temperature sensor, and the output end of the controller is connected with a nitrogen control valve, a vacuumizing control valve, a mixed gas control valve and a nitrogen control valve and a vacuumizing control valve in a gas control system II in a plasma generator, a sample carrying table, a culture system and the gas control system I.
The sample carrying table is an automatic rotating and lifting sample carrying table.
Furthermore, the automatic rotation lifting sample carrying platform controls the lifting or horizontal rotation of the stepping motor sample carrying platform through the controller so as to automatically process a plurality of samples.
Plasma anaerobic microorganism breeding device still includes lighting system, sterilization system.
Further, the lighting system and the sterilization system are arranged on the inner wall of the operation chamber.
The lighting system and the sterilization system can be controlled by the controller, and parameters are set on the operation panel.
The sampling chamber and the operation chamber are arranged in the shell, and a plasma generation gas working gas source, a mixed gas source, a nitrogen source, a main power switch, a power connector and a water injection port are arranged on one side outside the shell.
The operation window of the operation room visual shop window consists of two operation windows.
The bottom of the gas catalysis module is provided with a fan, and the catalyst, the drying agent and the high-efficiency gas purifying material are sequentially arranged from bottom to top.
The high-efficiency gas purifier comprises activated carbon.
The utility model discloses technical effect as follows:
according to the utility model discloses a plasma anaerobe breeding device eliminates oxygen through nitrogen control valve, evacuation control valve, mist control valve, gaseous catalysis module control valve and acts on the anaerobe poison.
The utility model discloses a plasma anaerobic microorganism breeding device can be according to the time of advancing nitrogen gas volume, evacuation on the operating panel to and the air input of mist, including gaseous catalytic module, come effective control oxygen content to maintain below 0.03% for a long time.
Simultaneously the utility model discloses plasma anaerobic microorganism breeding device is accomplishing the mutagenesis operation back, can place anaerobic microorganism and cultivate in the culture system in the device, has reduced and has taken out the time of keeping somewhere in the external world with the sample, and less oxygen poisons the possibility, and the mutagenesis is cultivateed with the anaerobism simultaneously and is combined together, makes the experiment operation more simple and easy, more is favorable to the breeding effect of mutagenesis and cultivation.
Drawings
FIG. 1 is a schematic view of the overall structure of an embodiment of the plasma anaerobic microorganism breeding device of the present invention.
FIG. 2 is a structural view of an embodiment of the plasma anaerobic microorganism breeding apparatus of the present invention;
FIG. 3 is a front view of an embodiment of the plasma anaerobic microorganism breeding device of the present invention;
FIG. 4 is a side view of an embodiment of the plasma anaerobic microorganism breeding device of the present invention;
description of the symbols:
1, a shell; 2, an operation chamber; 3, sampling a chamber; 4, a visible front show window; 5, a generator;
6 automatically rotating the carrier; 7 a culture system; 8, an illumination system; 9, a sterilization system;
10 an operation panel; 11 a pass gate; 12 automatically rotating and lifting the carrying platform;
13, an operation window of the visible front show window; 14 a sampling chamber door;
15 working gas source interface of plasma generator; 16 a mixed gas source interface;
17 nitrogen source interface; 18 a mains power switch; 19 water injection port and water outlet;
20 a power interface; 21 an anaerobic microorganism operation treatment module; 22 a detection and control module;
23 gas control system I; 24 a gas catalytic module; 25 radio frequency power supply module;
26 a source of working gas; 27 gas control system II; 28 a controller;
29 a gas flow controller; 30 a gas controller; 31 a power sensor;
a 32 oxygen sensor; 33 a temperature sensor; 34 stepping motor.
Detailed Description
Specific embodiments of the present invention will be described in more detail below with reference to the accompanying drawings.
The utility model discloses a plasma anaerobic microorganism breeding device for under the ordinary pressure room temperature condition through the mutagenesis treatment and the cultivation of plasma technique to anaerobic microorganism, like figure 1 and figure 2, including anaerobic microorganism operation processing module, detection and control module, anaerobic microorganism operation processing module includes: an operation chamber and a sampling chamber connected with the operation chamber through a transfer door,
the operating room is internally provided with a plasma generator, a sample carrying table, an operating room visual show window, a culture system, a gas control system I and a gas catalysis module, wherein the plasma generator is opposite to the sample carrying table and is connected with the plasma control system on the upper part of the operating room, the plasma generator is connected with a working gas source and a radio frequency power supply module, the operating room visual show window is made of transparent materials, an operating window is arranged below the operating window, the operating window is provided with sealed operating gloves, the culture system can realize a constant-temperature culture device, the gas control system I comprises a nitrogen control valve, a vacuumizing control valve and a mixed gas control valve, and the gas catalysis module is a functional module which is arranged in the operating room and can eliminate residual oxygen and gas generated by microbial metabolism;
the sampling chamber is provided with a sampling chamber door, and a gas control system II which comprises a nitrogen control valve and a vacuumizing control valve is arranged in the sampling chamber;
the detection and control module comprises a gas flow controller for controlling a working gas source, a gas controller for controlling a gas control system I and a gas control system II, a power sensor for detecting the power of the radio frequency source power supply module, oxygen sensors respectively arranged in the operation chamber and the sampling chamber and used for detecting the oxygen environment, a temperature sensor for detecting the temperature of the culture system, an operation panel for setting parameters, and a controller, the input end of the controller is connected with an operation panel, a gas flow controller, a gas controller, a radio frequency source power supply module power sensor, an oxygen sensor and a temperature sensor, and the output end of the controller is connected with a nitrogen control valve, a vacuumizing control valve, a mixed gas control valve and a nitrogen control valve and a vacuumizing control valve in a gas control system II in a plasma generator, a sample carrying table, a culture system and the gas control system I.
The utility model provides a carry the appearance platform and carry the appearance platform for the autogiration lift, it links to each other with step motor, controls the lift or the horizontal rotation of step motor objective table in order to carry out automatic processing to a plurality of samples through the controller.
The utility model discloses a plasma anaerobic microorganism breeding device still includes lighting system, sterilization system. The illumination system can adopt a fluorescent lamp and the like for illumination, and the sterilization system can adopt an ultraviolet lamp for sterilization, and the ultraviolet lamp is connected with the controller; or the pupil of the supply pipeline arranged on the inner wall can be sterilized by an externally connected chemical sterilizing gas supply device. The lighting system and the sterilization system can be controlled by the controller, and parameters are set on the operation panel.
The plasma generator of the utility model is connected with a radio frequency power supply module, a cooling system and a working air source system, and the plasma generator converts the working air source system such as helium gas into plasma jet by utilizing the energy provided by the radio frequency power supply module; the temperature of the plasma generator can also rise along with the prolonging of the working time, the cooling system is sequentially connected with the water pump, the water tank and the heat exchanger, and two ends of the plasma generator are respectively connected with the water pump and the radiator or the cooling system is connected with the cooling water circulator. The cooling system cools the plasma generator through water cooling or air cooling, and the plasma generator stably emits room-temperature plasma jet under the monitoring of the temperature sensor of the plasma generator. The plasma generator of the utility model can be a plasma generator with a coaxial structure, and the plasma generator with the coaxial structure adopts coaxial bare metal to generate plasma jet; the plasma generator of the present invention may also be a flat plate type plasma generator, and for example, refer to the plasma generator described in the patent of CN100405879C invention as an embodiment of the flat plate type plasma generator.
In one embodiment, the operation chamber and the sampling chamber are independent strictly sealed space structures, the operation chamber and the sampling chamber are communicated through a transfer door, the transfer door is in a closed state normally, and the transfer door is opened during the process of placing and taking out the sample. The anaerobic environment is manufactured in the sampling chamber through the gas control system II, the sampling chamber plays a buffering role, the anaerobic environment of the sampling chamber is favorably maintained, and the time spent on recovering the anaerobic environment is reduced.
The utility model discloses a plasma anaerobic microorganism breeding device's another embodiment, carry the appearance platform and carry the appearance platform for the autogiration lift, its lift or horizontal rotation that come control step motor objective table through the controller are in order to carry out automatic handling to a plurality of samples. And controlling the rotation speed of the object stage, so that the time interval for automatically replacing the next sample to be processed after the sample is processed is within a fixed time, such as 5-10 s. The continuous automatic processing of batch samples is realized, the complex work of manually placing and taking out the samples in the prior art is avoided, and the mutation breeding efficiency is improved.
The utility model discloses a plasma anaerobic microorganism breeding device's another embodiment, sample room and control chamber set up in the casing, one side of casing is provided with plasma generation gas work air supply, mixed air supply, nitrogen gas source, total switch, power connection, water filling port, like figure 3. The plasma generation gas working gas source enters the plasma generator through the gas storage bottle and the like, and under the action of the plasma generator, plasma jet flow is generated and flows out from the emission surface of the plasma generator, which is opposite to the automatic rotating and lifting sample carrying table, and acts on a sample to carry out mutagenesis treatment. The mixed gas source and the nitrogen source are separately piped with corresponding gases, e.g. the mixed gas is 10% H2+5%CO2+85%N2The nitrogen gas is 99.999% purity nitrogen gas, and the gas pipeline is controlled by a nitrogen gas control valve and a mixed gas control valve at one end of the operation chamber. The power connector is connected with the power supply between the power system and the equipment, and the main power switch controls the main power supply of the plasma anaerobic microorganism breeding device. The cooling device of the cooling system is such as a cooling water circulator, cooling water is connected with the plasma generator through a water filling port pipeline, and flows out of the water outlet pipe and the water outlet after flowing and cooling of the plasma generator.
In another embodiment of the plasma anaerobic microorganism breeding device of the present invention, the operation window of the operation room visible show window shown in fig. 2 is composed of two windows, so that the operation mode is more humanized.
The utility model discloses a plasma anaerobic microorganism breeding device's another embodiment, the temperature of culture system is steerable at 25-60 ℃, and culture system forms independent space alone, for example the box, and the box is provided with the box door, can set up temperature sensor, gas concentration sensor, humidity transducer in it.
The utility model discloses a plasma anaerobic microorganism breeding device's another embodiment, gaseous catalysis module bottom be provided with the fan, from upwards having set gradually catalyst, drier, high-efficient gaseous purification thing for example active carbon down. The fan can accelerate the gas circulation, accelerate the catalysis and drying treatment, and accelerate the elimination of residual oxygen and gas generated by microorganism metabolism by high-efficiency gas purifiers such as activated carbon.
The utility model discloses realize anaerobic microorganism's ordinary pressure room temperature plasma mutagenesis and anaerobism cultivation integration, after accomplishing the mutagenesis operation, can place anaerobic microorganism and cultivate in the culture system in the device, reduced and taken out the time of keeping somewhere in the external world with the sample, less oxygen poisons the possibility, the simultaneous mutagenesis is in the same place with the anaerobism cultivation combination, more is favorable to the breeding effect of mutagenesis and cultivation.
The utility model discloses plasma anaerobic microorganism breeding device has filled the plasma technique in the scope that anaerobic microorganism used, possesses the advantage that has the ion beam mutagenesis to it is high-efficient, safer, the equipment operation is more convenient to change suddenly.
The utility model discloses because of having above-mentioned structure, can realizing the integration operation of mutagenesis and cultivation, reduced and taken out the sample and kept somewhere in the external world the time, less oxygen poisons the possibility of toxicity, the mutagenesis is cultivateed with the anaerobism simultaneously and is combined together, more is favorable to the breeding effect of mutagenesis and cultivation.
Industrial applicability
The utility model discloses a plasma anaerobic microorganism breeding device can make and use in anaerobic microorganism plasma mutation breeding field.
Although the embodiments of the present invention have been described above with reference to the accompanying drawings, the present invention is not limited to the above-described embodiments and application fields, and the above-described embodiments are illustrative, instructive, and not restrictive. Those skilled in the art, having the benefit of this disclosure, may effect numerous modifications thereto without departing from the scope of the invention as defined by the appended claims.
Claims (10)
1. The utility model provides a plasma anaerobic microorganism breeding device, includes anaerobic microorganism operation processing module, detects and the control module, its characterized in that:
the anaerobic microorganism operation treatment module comprises: an operation chamber and a sampling chamber connected with the operation chamber through a transfer door,
the operating room is internally provided with a plasma generator, a sample carrying table, an operating room visual show window, a culture system, a gas control system I and a gas catalysis module, wherein the plasma generator is opposite to the sample carrying table and is connected with the plasma control system on the upper part of the operating room, the plasma generator is connected with a working gas source and a radio frequency power supply module, the operating room visual show window is made of transparent materials, an operating window is arranged below the operating window, the operating window is provided with sealed operating gloves, the culture system can realize a constant-temperature culture device, the gas control system I comprises a nitrogen control valve, a vacuumizing control valve and a mixed gas control valve, and the gas catalysis module is a functional module which is arranged in the operating room and can eliminate residual oxygen and gas generated by microbial metabolism;
the sampling chamber is provided with a sampling chamber door, and a gas control system II which comprises a nitrogen control valve and a vacuumizing control valve is arranged in the sampling chamber;
the detection and control module comprises a gas flow controller for controlling a working gas source, a gas controller for controlling a gas control system I and a gas control system II, a power sensor for detecting the power of the radio frequency source power supply module, oxygen sensors respectively arranged in the operation chamber and the sampling chamber and used for detecting the oxygen environment, a temperature sensor for detecting the temperature of the culture system, an operation panel for setting parameters, and a controller, the input end of the controller is connected with an operation panel, a gas flow controller, a gas controller, a radio frequency source power supply module power sensor, an oxygen sensor and a temperature sensor, and the output end of the controller is connected with a nitrogen control valve, a vacuumizing control valve, a mixed gas control valve and a nitrogen control valve and a vacuumizing control valve in a gas control system II in a plasma generator, a sample carrying table, a culture system and the gas control system I.
2. The plasma anaerobic microorganism breeding device according to claim 1, wherein the sample stage is an automatic rotating and lifting sample stage.
3. The apparatus according to claim 2, wherein the automatic rotation/elevation stage is a controller for controlling elevation or horizontal rotation of a stepping motor stage to automatically process a plurality of samples.
4. The plasma anaerobic microorganism breeding device according to any one of claims 1 to 3, further comprising a lighting system and a sterilization system.
5. The plasma anaerobic microorganism breeding device according to claim 4, wherein the lighting system and the sterilization system are arranged on the inner wall of the operation chamber.
6. The apparatus of claim 4, wherein the lighting system and the sterilization system are controlled by a controller, and parameters are set on an operation panel.
7. The plasma anaerobic microorganism breeding device according to claim 1, wherein the sampling chamber and the operation chamber are arranged in a shell, and a plasma generating gas working gas source, a mixed gas source, a nitrogen gas source, a main power switch, a power connector and a water filling port are arranged on one side outside the shell.
8. The apparatus for breeding plasma anaerobic microorganisms according to claim 1, wherein the operation window of the operation chamber visual shop window is composed of two.
9. The plasma anaerobic microorganism breeding device as claimed in claim 1, wherein a fan is arranged at the bottom of the gas catalysis module, and a catalyst, a drying agent and a high-efficiency gas purifying material are sequentially arranged from bottom to top.
10. The plasma anaerobic microorganism breeding device according to claim 9, wherein the high efficiency gas purifier comprises activated carbon.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920892676.4U CN210736727U (en) | 2019-06-14 | 2019-06-14 | Plasma anaerobic microorganism breeding device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920892676.4U CN210736727U (en) | 2019-06-14 | 2019-06-14 | Plasma anaerobic microorganism breeding device |
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| CN210736727U true CN210736727U (en) | 2020-06-12 |
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| CN201920892676.4U Active CN210736727U (en) | 2019-06-14 | 2019-06-14 | Plasma anaerobic microorganism breeding device |
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