CN112697554A - Soil microorganism fumigation device and fumigation method - Google Patents
Soil microorganism fumigation device and fumigation method Download PDFInfo
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- CN112697554A CN112697554A CN202011475849.6A CN202011475849A CN112697554A CN 112697554 A CN112697554 A CN 112697554A CN 202011475849 A CN202011475849 A CN 202011475849A CN 112697554 A CN112697554 A CN 112697554A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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Abstract
The invention belongs to the technical field of soil microorganism detection equipment, and particularly relates to a soil microorganism fumigating device, which comprises: the device comprises a refrigerating device (1), a heating device (2), an air blower (3), a vacuum pump (4), a plurality of electromagnetic valves (5), an exhaust device (6), a PLC (programmable logic controller) controller (7), a cabinet body (8), a cabinet door (9), a fumigation pot (10) and a buzzer (24); a storage space is arranged in the cabinet body (8) and is used as a placing chamber, and a fumigation pot (10) is placed in the placing chamber; an interlayer placing cavity is additionally arranged between the placing chamber and the bottom end of the cabinet body (8), and a refrigerating device (1), a heating device (2), an air blower (3) and a vacuum pump (4) are arranged in the interlayer placing cavity; an air outlet is arranged in the middle of the top of the placing chamber, and an air exhaust device (6) is arranged at the air outlet; a PLC controller (7), a buzzer (24) and a power switch (21) are arranged between the top of the placing chamber and the top end of the cabinet body (8).
Description
Technical Field
The invention belongs to the technical field of soil microorganism detection equipment, and particularly relates to a soil microorganism fumigation device and a fumigation method.
Background
Soil microorganisms are one of the three major phyla of the earth, and the soil science refers to organisms living in soil with a volume of less than 5X103 micro cubic meters as soil microorganisms. Soil microorganisms play a role of final decomposers in the earth ecosystem, are not only various (bacteria, fungi, actinomycetes, protozoa and the like), but also play a key role in the conversion and circulation of soil organic matters and nutrient elements such as nitrogen, phosphorus, sulfur and the like. The determination of soil microbial biomass is an important means in current soil science and ecology in the aspects of researching soil microbial structures, functions and the like.
At present, the traditional determination method for soil microbial biomass is popularized and widely applied in extensive laboratories all over the world, soil samples need to be fumigated when determining soil microbial biomass carbon nitrogen, soil microbial fumigation generally includes that after fresh soil samples are fumigated by chloroform, microbial dead cells in soil are cracked to release microbial biomass carbon, soil is extracted by using a certain volume of LK2SO4 solution, the microbial biomass carbon content is determined by using an organic carbon automatic analyzer, and the difference value and the conversion coefficient of organic carbon are determined according to fumigated soil and non-fumigated soil, SO that the soil microbial carbon biomass is calculated. The requirements of the experimental operation process and the experimental conditions are very strict, tiny operation errors and culture errors easily cause experimental failure, and in the microbial culture and fumigation processes, a glass drying dish needs to be sealed by paraffin, the sealing performance is poor, the constant-temperature culture temperature is difficult to control, the vacuum pump is required to be manually utilized to pump vacuum in the culture process, a certain negative pressure is kept, the capacity of a glass dryer is limited, the same batch of samples are difficult to manually maintain the consistency of the negative pressure, the difference of the sample fumigation effect is large, the experimental success rate of the traditional fumigation method is extremely low, after the fumigation is finished, the chloroform in the container is removed quite unsatisfactorily, the vacuum pump is used for repeatedly pumping air, the chloroform in the container cannot be completely removed, and time and labor are wasted.
Disclosure of Invention
In order to solve the defects in the prior art, the invention provides a soil microorganism fumigation device and a fumigation method, in particular to a device for full-automatic fumigation of soil microorganism biomass, which comprises the following steps: the device comprises a refrigerating device, a heating device, an air blower, a vacuum pump, a plurality of electromagnetic valves, an exhaust device, a PLC (programmable logic controller), a cabinet body, a cabinet door, a fumigation pot and a buzzer;
a storage space is arranged in the cabinet body and is used as a placing chamber, a cabinet door is arranged on one side of the placing chamber, and a fumigation pot is arranged in the placing chamber; an interlayer placing cavity is additionally arranged between the placing chamber and the bottom end of the cabinet body, and a refrigerating device, a heating device, a blower and a vacuum pump are arranged in the interlayer placing cavity;
the air blower is positioned above an air inlet formed in the middle of the bottom of the placing chamber, the air blower is connected with the air inlet through a first connecting pipe, and the rubber pipe is provided with an electromagnetic valve;
the vacuum pump is communicated with the fumigation pot through a rubber pipe, and an electromagnetic valve is arranged on the rubber pipe;
an air outlet is arranged in the middle of the top of the placing chamber, and an air exhaust device is arranged at the air outlet;
a PLC controller, a buzzer and a power switch are arranged between the top of the placing chamber and the top of the cabinet body.
As an improvement of the above technical solution, the fumigation tank includes: the upper cover, the fumigation pot body and the plurality of hollowed-out partition plates;
the plurality of hollow-out clapboards are arranged in the fumigation pot body in a laminated structure, and the upper cover covers the fumigation pot body to form a sealed space; a beaker filled with chloroform and a plurality of sample bottles are arranged in the middle of each hollow clapboard; a plurality of sample bottles are distributed around a beaker filled with chloroform in a circumferential distribution manner;
the upper cover is provided with a digital pressure gauge and a second connecting pipe; the digital pressure gauge is used for measuring the pressure value in the fumigation tank and controlling the sample culture negative pressure in the sample bottle according to the measured pressure value; the second connecting pipe is provided with an electromagnetic valve and is communicated with the vacuum pump through a rubber pipe.
As one improvement of the technical scheme, a rubber sealing ring is arranged on the inner side of the lower edge of the upper cover, and the air tightness between the upper cover and the fumigation pot body is increased.
As one of the improvement of the technical scheme, the upper cover is of a round table-shaped structure, the cross section of the upper cover is of an isosceles trapezoid shape, the upper cover and the fumigation pot body are connected through a buckle, the two sides of the upper edge of the fumigation pot body are respectively provided with a safety buckle, and the upper cover is further buckled after the sealing compression nut is pressed down, so that the upper cover is hooked, and the upper cover and the fumigation pot body are further compressed.
As one improvement of the technical scheme, the hollow-out partition plate is of a hollow-out structure with an opening at the top end and a U-shaped cross section, and is made of heat conducting materials.
As one of the improvement of above-mentioned technical scheme, the top of the cabinet body sets up the touch-sensitive screen, and this touch-sensitive screen is connected with bee calling organ, user terminal and PLC controller respectively through wireless network, and the PLC controller passes through long distance communication mode and user terminal remote connection, and simultaneously, the PLC controller still with bee calling organ electric connection.
As one improvement of the above technical solution, the user terminal is a mobile phone, a computer or a tablet computer.
The invention also provides a soil microorganism fumigation method, which comprises the following steps:
a beaker filled with chloroform is placed in the middle of each hollow-out partition plate, a plurality of sample bottles are distributed around the beaker filled with chloroform in a circumferential manner, and the hollow-out partition plates are stacked together and placed in a fumigation tank;
the PLC controls the electromagnetic valve to be opened, the vacuum pump vacuumizes the fumigation tank, and the digital pressure gauge displays the pressure value in the fumigation tank in real time, so that the culture negative pressure in each sample bottle is determined;
according to the pressure value displayed by the digital pressure gauge in real time, the PLC controls the on-off of an electromagnetic valve arranged on the rubber tube, and adjusts the pressure value in the fumigation tank to always maintain the culture negative pressure according with the soil sample in the sample bottle;
heating the fumigation tank by using a heating device so as to heat the soil sample in each sample bottle in the fumigation tank, and fumigating the soil sample in each sample bottle by using chloroform in a beaker filled with chloroform and arranged in the middle of the hollow partition plate;
the PLC controls the on-off of the refrigerating device according to the heating temperature of the heating device, so that the soil sample in each sample bottle in the fumigation tank is always kept at the culture temperature of the soil sample;
the PLC judges whether the pressure and the temperature in the fumigation tank need to be adjusted or not according to the culture temperature and the culture negative pressure of each soil sample which are respectively displayed on the touch screen and the user terminal in real time;
if the culture temperature of the soil sample displayed in real time is greater than or equal to a preset temperature threshold value and the culture negative pressure of the soil sample displayed in real time is greater than or equal to a preset pressure threshold value, the PLC controls the heating device and the refrigerating device to adjust the temperature in the fumigation tank to be in accordance with the culture temperature of the soil sample; the PLC controls an electromagnetic valve arranged on the rubber tube, adjusts the gas flow rate, and adjusts the pressure in the fumigation tank to the culture negative pressure according with the soil sample;
if the culture temperature of the soil sample displayed in real time is smaller than a preset temperature threshold value or the culture negative pressure of the soil sample displayed in real time is smaller than a preset pressure threshold value, the PLC sends an instruction to the buzzer, an alarm is sent to the touch screen and the user terminal, an operator can respond in time, and the pressure and the temperature in the fumigation tank are adjusted by the PLC through the user terminal or the touch screen until the culture temperature and the culture negative pressure which accord with the soil sample are reached;
the PLC controller collects the operation time of the whole device;
after the culture is finished, the PLC controller controls the electromagnetic valve connected with the first connecting pipe to be opened, meanwhile, the upper cover and the sealing cover are opened, the air blower starts to work, chloroform in the fumigation bottle is blown out of the fumigation bottle, the air exhaust device is started, and the chloroform blown out of the fumigation bottle is discharged out of the cabinet body.
Compared with the prior art, the invention has the beneficial effects that:
1. when the device is used for fumigating, the culture negative pressure and the culture temperature of the soil sample in each sample bottle can be ensured, the operation by experience is not needed, the automatic and unmanned real-time monitoring is realized in the whole process, the culture negative pressure and the culture temperature of the soil sample in each sample bottle can be monitored in real time by utilizing the PLC, the touch screen and the user terminal, and the timely and corresponding adjustment is made to ensure that the fumigating is smoothly carried out;
2. after the device disclosed by the invention is used for fumigating, the chloroform removal speed is high, the removal effect is obvious, the removal mode is convenient and fast, the capacity of the soil sample which can be loaded is large, the time cost is greatly saved, and the working efficiency is improved.
Drawings
FIG. 1 is a schematic structural diagram of a soil microorganism fumigation device of the present invention;
fig. 2 is a schematic structural diagram of a user terminal of the soil microorganism fumigation device of the invention.
Reference numerals:
1. refrigerating device 2 and heating device
3. Blower 4, vacuum pump
5. Electromagnetic valve 6, exhaust device
7. PLC controller 8, cabinet body
9. Cabinet door 10 and fumigation tank
11. Upper cover 12 and fumigation pot body
13. Hollow partition plate 14 and beaker filled with chloroform
15. Sample bottle 16, first connecting tube
17. Digital pressure gauge 18 and second connecting pipe
19. Rubber tube 20 and sealing compression nut
21. Power switch 22, safety buckle
23. User terminal 24, buzzer
Detailed Description
The invention will now be further described with reference to the accompanying drawings.
As shown in fig. 1, the present invention provides a soil microorganism fumigation apparatus, comprising: the device comprises a refrigerating device 1, a heating device 2, an air blower 3, a vacuum pump 4, a plurality of electromagnetic valves 5, an exhaust device 6, a PLC (programmable logic controller) 7, a cabinet body 8, a cabinet door 9, a fumigation tank 10 and a buzzer 24;
a storage space is arranged in the cabinet body 8 and is used as a placing chamber, a cabinet door 9 is arranged on one side of the placing chamber, and a fumigation pot 10 is arranged in the placing chamber; before starting fumigation, the cabinet door 9 is opened, the fumigation tank is placed in the placing chamber, and the cabinet door 9 is closed; after the fumigation is finished, taking the fumigation pot out of the placing chamber; an interlayer placing cavity is additionally arranged between the placing chamber and the bottom of the cabinet body 8, and a refrigerating device 1, a heating device 2, an air blower 3 and a vacuum pump 4 are arranged in the interlayer placing cavity;
the air blower 3 is positioned above an air inlet arranged in the middle of the bottom of the placing chamber, the air blower 3 is connected with the air inlet through a first connecting pipe 16, and the rubber pipe is provided with an electromagnetic valve 5;
the vacuum pump 4 is communicated with the fumigation tank through a rubber pipe and is used for vacuumizing the fumigation tank, and the rubber pipe is provided with an electromagnetic valve 5;
an air outlet is arranged in the middle of the top of the placing chamber, and an air exhaust device 6 is arranged at the air outlet; wherein, in the embodiment, the exhaust device is an exhaust fan;
a PLC (programmable logic controller) 7, a buzzer 24 and a power switch 21 are arranged between the top of the placing chamber and the top end of the cabinet body 8, and the PLC is used for adjusting the on-off of the corresponding electromagnetic valve 5 and controlling the speed of the gas flow flowing through the corresponding electromagnetic valve 5 according to the gas flow flowing through the electromagnetic valve 5; the temperature control device is also used for controlling the temperature values generated by the heating device 2 and the refrigerating device 1 respectively according to the culture temperatures collected by the heating device 2 and the refrigerating device 1;
wherein, in the embodiment, the refrigeration device is a compressor; the heating device is formed by combining one or more groups of heating wires and a fan, each group of heating wires is positioned above the fan, when each group of heating wires is electrified, the heating wires can generate heat, a motor in the fan drives a rotor to drive a fan blade to rotate, and the air blown out by the fan is changed into hot air through the heating wires and is sent to the blower 3;
the power switch 21 is used for controlling the on-off of the power supply, providing the power supply required by the normal operation for each component in the whole device when the power switch is closed, and stopping providing the power supply required by the normal operation for each component in the whole device when the power switch is disconnected.
Wherein, install air-blower 3 additional and can realize getting rid of the purpose of chloroform, install exhaust device 6 additional and can realize discharging the inside chloroform of fumigation jar 10 outside the cabinet body 8.
Wherein, the fumigation tank 10 includes: the upper cover 11, the fumigation pot body 12 and a plurality of hollowed-out partition plates 13;
the plurality of hollow partition plates 13 are arranged in the fumigation pot body 12 in a laminated structure, and the upper cover 11 covers the fumigation pot body 12 to form a sealed space; a beaker 14 filled with chloroform and a plurality of sample bottles 15 are arranged in the middle of each hollow clapboard 13; a plurality of sample bottles 15 are distributed around the beaker 14 containing chloroform in a circumferential distribution;
the upper cover 11 is provided with a digital pressure gauge 17 and a second connecting pipe 18; the digital pressure gauge 17 is used for measuring the pressure value in the fumigation tank 10 and controlling the sample culture negative pressure in the sample bottle 15 according to the measured pressure value; the second connecting pipe 18 is provided with an electromagnetic valve 5 and is communicated with the vacuum pump 4 through a rubber pipe 19. Wherein, can realize through installing digital pressure table 17 additional that the soil sample in every batch of sample bottle 15 is when stifling, the negative pressure is unanimous, and stifling effect is unanimous, reduces experimental error.
The hollow partition plate 13 is a hollow structure with an opening at the top end and a U-shaped cross section, and the hollow partition plate 13 is made of a heat conducting material.
Wherein, the inner side of the lower edge of the upper cover 11 is provided with a rubber sealing ring, so that the air tightness between the upper cover 11 and the fumigation pot body 12 is increased. In this embodiment, the upper cover 11 is an explosion-proof glass upper cover.
The upper cover 11 is a round platform structure, the cross section of the upper cover is isosceles trapezoid, the upper cover 11 is connected with the fumigation pot body 12 in a buckling manner, the two sides of the upper edge of the fumigation pot body 12 are respectively provided with a safety buckle 22, and the upper cover 11 is further buckled after the sealing compression nut 20 is pressed down, so that the upper cover 11 is hooked, the upper cover 11 and the fumigation pot body 12 are further compressed, and the safety of the whole fumigation device is improved.
In other embodiments, the upper cover 11 is a round cover structure, and a sealing compression nut 20 and a sealing cover are further arranged on the round cover structure; the sealing cover is sleeved outside the upper cover 11, the middle part of the sealing cover is provided with a sealing compression nut 20, and when the sealing compression nut 20 is screwed down, the sealing cover 21 can be driven to be pressed down on the upper cover 11; meanwhile, the safety buckles 22 are respectively arranged on two sides of the upper edge of the fumigation pot body 12, and after the sealing compression nut 20 is pressed down, the sealing cover is buckled, so that the upper cover 11 is hooked, the upper cover 11 and the fumigation pot body 12 are compressed, and the safety of the whole fumigation device is improved.
Wherein, the top of the cabinet body 8 sets up the touch-sensitive screen, and this touch-sensitive screen is connected with bee calling organ 24, user terminal 23 and PLC controller 7 respectively through wireless network, and the PLC controller passes through long distance communication mode and user terminal 23 remote connection, and simultaneously, PLC controller 7 still with bee calling organ 24 electric connection.
Specifically, the touch screen displays the pressure in the fumigation tank 10, the culture temperature and the culture negative pressure in each sample bottle 15 in real time; different keys are operated according to the displayed numerical values, corresponding instructions are sent to the PLC, and the PLC executes corresponding operations according to the corresponding instructions to control the pressure in the fumigation pot 10 and the culture temperature and culture negative pressure in each sample bottle 15; the PLC controller is remotely connected with the user terminal 23 in a long-distance communication mode, the culture temperature and the culture negative pressure in each sample bottle 15 are displayed on the user terminal in real time, an operator can check and send a control instruction to the PLC controller, and the pressure in the fumigation pot 10 and the culture temperature and the culture negative pressure in each sample bottle 15 are correspondingly controlled. The touch screen, the user terminal 23, the PLC 7 and the buzzer which are mutually connected are additionally arranged, so that remote automatic operation and timely alarm reminding can be realized.
The user terminal 23 is a mobile phone, a computer or a tablet computer.
As shown in fig. 1 and 2, the present invention also provides a soil microorganism fumigation method, comprising:
a beaker 14 filled with chloroform is placed in the middle of each hollow clapboard 13, a plurality of sample bottles 15 are distributed around the beaker 14 filled with chloroform in a circumferential manner, and the hollow clapboards 13 are stacked together and placed in the fumigation pot 10;
the PLC controller controls the electromagnetic valve 5 to be opened, the vacuum pump 4 vacuumizes the fumigation pot 10, and the digital pressure gauge 17 displays the pressure value in the fumigation pot 10 in real time, so that the culture negative pressure in each sample bottle 15 is determined;
according to the pressure value displayed by the digital pressure gauge 17 in real time, the PLC controller controls the on-off of the electromagnetic valve 5 arranged on the rubber tube 19, and adjusts the pressure value in the fumigation pot 10 to always maintain the culture negative pressure according with the soil sample in the sample bottle 15;
heating the fumigation pot 10 by using the heating device 2, thereby heating the soil sample in each sample bottle 15 placed in the fumigation pot 10, and fumigating the soil sample in each sample bottle 15 by using chloroform in a beaker 14 filled with chloroform and placed in the middle of the hollow partition plate 13;
the PLC 7 controls the on-off of the refrigerating device 1 according to the heating temperature of the heating device 2, so that the soil sample in each sample bottle 15 placed in the fumigation pot 10 always keeps the culture temperature according with the soil sample;
the PLC 7 judges whether the pressure and the temperature in the fumigation pot 10 need to be adjusted according to the culture temperature and the culture negative pressure of each soil sample respectively displayed on the touch screen and the user terminal in real time;
if the culture temperature of the soil sample displayed in real time is greater than or equal to the preset temperature threshold value and the culture negative pressure of the soil sample displayed in real time is greater than or equal to the preset pressure threshold value, the PLC controller controls the heating device 2 and the refrigerating device 1 to adjust the temperature in the fumigation tank 10 to be in accordance with the culture temperature of the soil sample; the PLC 7 controls the electromagnetic valve 5 arranged on the rubber tube 19, adjusts the gas flow rate, and adjusts the pressure in the fumigation pot 10 to the culture negative pressure according with the soil sample;
if the culture temperature of the soil sample displayed in real time is smaller than the preset temperature threshold value or the culture negative pressure of the soil sample displayed in real time is smaller than the preset pressure threshold value, the PLC 7 sends an instruction to the buzzer 24, an alarm is sent to the touch screen and the user terminal 23, an operator can respond in time, and the pressure and the temperature in the fumigation pot 10 are adjusted by the PLC 7 through the user terminal or the touch screen until the culture temperature and the culture negative pressure which accord with the soil sample are reached;
the PLC collects the running time of the whole device for subsequent calculation of soil microorganism biomass carbon;
after the culture is finished, the PLC controls the electromagnetic valve 5 connected with the first connecting pipe to be opened, meanwhile, the upper cover 11 and the sealing cover 21 are opened, the air blower 3 starts to work, chloroform in the fumigation bottle 10 is blown out of the fumigation bottle 10, the air exhaust device 6 is started, and the chloroform blown out of the fumigation bottle 10 is exhausted out of the cabinet body 8, so that the aim of removing the chloroform is achieved.
In the traditional fumigation method, the culture negative pressure of the soil sample in each batch of sample bottles in a closed container can not be kept consistent, the operation is carried out only by experience, the experimental error is very large, the experimental accuracy is low, and the fumigation is often failed, so that the success rate of completing the fumigation is greatly reduced; meanwhile, after the culture is finished, chloroform cannot be thoroughly removed, even though the vacuum pump is used for repeatedly pumping air, the chloroform cannot be thoroughly removed, and the operation is time-consuming and labor-consuming.
According to the fumigation method, when soil microbial biomass is fumigated, only a weighed soil sample is placed in a corresponding sample bottle, the soil sample is circumferentially distributed around a beaker 14 filled with chloroform and sodium hydroxide solution, a hollow partition plate provided with the beaker 14 and a plurality of sample bottles 15 is stacked in a fumigation bottle 10, a sealing cover and an upper cover are covered to achieve sealing, the operation before fumigation is completed by clicking a 'start key' on a touch screen, the pressure and the temperature in the fumigation bottle 10 are monitored in real time by using a PLC (programmable logic controller) 7, the touch screen or a user terminal is used for real-time adjustment until fumigation is completed, and the user terminal can receive a completion prompt after the fumigation is completed.
After the fumigation is finished, only the upper cover and the sealing cover need to be opened, a 'chloroform removal key' is clicked on the touch screen, the PLC controls the electromagnetic valve arranged on the first connecting pipe to be opened, the air blower 3 starts to work, chloroform is blown out of the fumigation bottle 10 and is discharged out of the cabinet body 8 through the air exhaust device 6, and the chloroform is taken away along with wind, so that the chloroform is completely removed. The method has the advantages of high chloroform removal speed, obvious removal effect, convenient removal mode, large capacity of the soil sample which can be loaded, great time and cost saving and improvement of the working efficiency.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and are not limited. Although the present invention has been described in detail with reference to the embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (8)
1. A soil microorganism fumigation apparatus, characterized in that, the apparatus includes: the device comprises a refrigerating device (1), a heating device (2), an air blower (3), a vacuum pump (4), a plurality of electromagnetic valves (5), an exhaust device (6), a PLC (programmable logic controller) controller (7), a cabinet body (8), a cabinet door (9), a fumigation pot (10) and a buzzer (24);
a storage space is arranged in the cabinet body (8) and is used as a placing chamber, a cabinet door (9) is arranged on one side of the placing chamber, and a fumigation pot (10) is arranged in the placing chamber; an interlayer placing cavity is additionally arranged between the placing chamber and the bottom end of the cabinet body (8), and a refrigerating device (1), a heating device (2), an air blower (3) and a vacuum pump (4) are arranged in the interlayer placing cavity;
the air blower (3) is positioned above an air inlet formed in the middle of the bottom of the placing chamber, the air blower (3) is connected with the air inlet through a first connecting pipe (16), and the rubber pipe is provided with an electromagnetic valve (5);
the vacuum pump (4) is communicated with the fumigation pot (10) through a rubber pipe (19), and an electromagnetic valve (5) is arranged on the rubber pipe;
an air outlet is arranged in the middle of the top of the placing chamber, and an air exhaust device (6) is arranged at the air outlet;
a PLC controller (7), a buzzer (24) and a power switch (21) are arranged between the top of the placing chamber and the top end of the cabinet body (8).
2. A soil microbial fumigation apparatus as defined in claim 1, wherein said fumigation tank (10) comprises: the upper cover (11), the fumigation pot body (12) and a plurality of hollowed-out partition plates (13);
the plurality of hollow partition plates (13) are arranged in the fumigation pot body (12) in a laminated structure, and the upper cover (11) covers the fumigation pot body (12) to form a sealed space; a beaker (14) filled with chloroform and a plurality of sample bottles (15) are arranged in the middle of each hollow clapboard (13); a plurality of sample bottles (15) are distributed around a beaker (14) filled with chloroform in a circumferential distribution manner;
a digital pressure gauge (17) and a second connecting pipe (18) are arranged on the upper cover (11); the digital pressure gauge (17) is used for measuring the pressure value in the fumigation tank (10) and controlling the sample culture negative pressure in the sample bottle (15) according to the measured pressure value; the second connecting pipe (18) is provided with an electromagnetic valve (5) and is communicated with the vacuum pump (4) through a rubber pipe (19).
3. A soil microbe fumigation device as defined in claim 2, wherein a rubber sealing ring is mounted on the inner side of the lower edge of said upper cover (11) to increase the air tightness between the upper cover (11) and the fumigation pot body (12).
4. A soil microorganism fumigation apparatus as defined in claim 2, wherein said upper cover (11) is a truncated cone-shaped structure, the cross section of which is isosceles trapezoid, the upper cover (11) is snap-fitted with the fumigation pot body (12), and the two sides of the upper edge of the fumigation pot body (12) are respectively provided with a safety buckle (22), after the sealing and pressing nut (20) is pressed down, the upper cover (11) is further buckled, so as to further press the upper cover (11) and the fumigation pot body (12).
5. The soil microorganism fumigating device according to claim 2, wherein the hollow partition plate (13) is a hollow structure with an opening at the top end and a U-shaped cross section, and the hollow partition plate (13) is made of a heat-conducting material.
6. The soil microorganism fumigating device according to claim 1, wherein a touch screen is arranged at the top end of the cabinet body (8), the touch screen is respectively connected with the buzzer (24), the user terminal (23) and the PLC (7) through a wireless network, the PLC (7) is remotely connected with the user terminal (23) through a long-distance communication mode, and meanwhile, the PLC (7) is electrically connected with the buzzer (24).
7. A soil microbial fumigation arrangement as defined in claim 6, in which said user terminal (23) is a mobile phone, a computer or a tablet computer.
8. A soil microorganism fumigation method, which is realized based on the soil microorganism fumigation device of any one of the claims 1-7, and is characterized in that the method comprises the following steps:
a beaker (14) filled with chloroform is placed in the middle of each hollow clapboard (13), a plurality of sample bottles (15) are distributed around the beaker (14) filled with chloroform in a circumferential distribution manner, and the hollow clapboards (13) are stacked together and are placed in a fumigation pot (10);
the PLC controller controls the electromagnetic valve (5) to be opened, the vacuum pump (4) vacuumizes the fumigation pot (10), the digital pressure gauge (17) displays the pressure value in the fumigation pot (10) in real time, and the culture negative pressure in each sample bottle (15) is determined;
according to the pressure value displayed by the digital pressure gauge (17) in real time, the PLC controller controls the on-off of the electromagnetic valve (5) arranged on the rubber tube (19), and adjusts the pressure value in the fumigation pot (10) to always maintain the culture negative pressure according with the soil sample in the sample bottle (15);
heating the fumigation pot (10) by using a heating device (2), thereby heating the soil sample in each sample bottle (15) placed in the fumigation pot (10), and fumigating the soil sample in each sample bottle (15) by using chloroform in a beaker (14) filled with chloroform and placed in the middle of a hollow partition plate (13);
the PLC (7) controls the on-off of the refrigerating device (1) according to the heating temperature of the heating device (2) so that the soil sample in each sample bottle (15) placed in the fumigation pot (10) is always kept at the culture temperature of the soil sample;
the PLC (7) judges whether the pressure and the temperature in the fumigation pot (10) need to be adjusted according to the culture temperature and the culture negative pressure of each soil sample which are respectively displayed on the touch screen and the user terminal (23) in real time;
if the culture temperature of the soil sample displayed in real time is greater than or equal to a preset temperature threshold value and the culture negative pressure of the soil sample displayed in real time is greater than or equal to a preset pressure threshold value, the PLC (7) controls the heating device (2) and the refrigerating device (1) to adjust the temperature in the fumigation tank (10) to be in accordance with the culture temperature of the soil sample; the PLC (7) controls an electromagnetic valve (5) arranged on the rubber tube (19) to adjust the gas flow rate and adjust the pressure in the fumigation pot (10) to the culture negative pressure according with the soil sample;
if the culture temperature of the soil sample displayed in real time is smaller than a preset temperature threshold value or the culture negative pressure of the soil sample displayed in real time is smaller than a preset pressure threshold value, the PLC (7) sends an instruction to the buzzer (24), sends an alarm prompt to the touch screen and the user terminal (23), and adjusts the pressure and the temperature in the fumigation tank (10) through the user terminal (23) or the touch screen by using the PLC (7) until the culture temperature and the culture negative pressure which accord with the soil sample are reached;
the PLC (7) collects the running time of the whole device;
after the culture is finished, the PLC (7) controls the electromagnetic valve (5) connected with the first connecting pipe (16) to be opened, meanwhile, the upper cover (11) and the sealing cover (21) are opened, the air blower (3) starts to work, chloroform in the fumigation bottle (10) is blown out of the fumigation bottle (10), the air exhaust device (6) is started, and the chloroform blown out of the fumigation bottle (10) is discharged out of the cabinet body (8).
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| CN202011475849.6A CN112697554A (en) | 2020-12-15 | 2020-12-15 | Soil microorganism fumigation device and fumigation method |
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| CN202011475849.6A CN112697554A (en) | 2020-12-15 | 2020-12-15 | Soil microorganism fumigation device and fumigation method |
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