CN210711351U - A high-efficiency screening device for monoclonal antibodies against secretin - Google Patents

Abstract

The utility model discloses a high-efficient sieving mechanism of monoclonal antibody of secretin, the device comprises a device shell, be located the first equipment district on device shell upper portion, the master control case, be located the second equipment district in the middle of the device shell and be located the third equipment district of device shell lower extreme, the inside in first equipment district is provided with the one-level filter cylinder, the one-level filter cylinder includes steel pipe and supporting network, the top of steel pipe is provided with the supporting network, and the inside of supporting network is provided with the filter membrane, the hollow top in inside of steel pipe is provided with the bleeder valve, the top of supporting network is provided with sealed lid, the top of one-level filter cylinder is provided with sharp driver, and sharp driver's drive end extends to sealed inslot, sharp driver's extension end is provided with the pressure plate, the top of one-level filter cylinder still is provided with the feed inlet. Has the advantages that: the cell material sizes of different micron levels can be screened and classified, the screening is more efficient and thorough, and the screening time is saved.

Description

A high-efficiency screening device for monoclonal antibodies against secretin

technical field

The utility model relates to the field of screening devices, in particular to a high-efficiency screening device for secretin monoclonal antibodies.

Background technique

Prolactin is a protein hormone secreted by the pituitary gland, and structurally it belongs to the same protein family as growth hormone and placental prolactin. PRL contains 199 amino acids, which are 26% and 27% identical to the amino acid sequences of growth hormone and placental prolactin, respectively. origin. Monoclonal antibodies are highly homogeneous antibodies produced by a single B cell clone directed against only a specific epitope. It is usually prepared by hybridoma technology. Hybridoma antibody technology is based on cell fusion technology, which fuses sensitized B cells with the ability to secrete specific antibodies and myeloma cells with unlimited reproduction ability into B cell hybridomas. Determination of PRL can be used as an important index to assess hypothalamic-pituitary function, but it is only contained in women, and the content in sterile women is very small. The study of monoclonal antibodies to secretin can be used for the etiological diagnosis of abnormal menstruation and infertility. Identification is of great significance.

In the production process, a centrifuge is often used to screen the antibody cells initially. Most of the existing centrifuge screening only uses the rotation of the centrifuge bucket to screen the cells and discards the cells containing mucus, red blood cell debris and other necrotic cell components. However, due to the different density and particle size of red blood cell debris and other necrotic cell components in the supernatant, the filter membrane of the same particle size cannot completely screen out antibody cells and other useful substances, which leads to the efficiency of cell screening. Low, the screening is not thorough.

In view of the problems in the related art, we propose a high-efficiency screening device for secretin monoclonal antibodies.

Utility model content

(1) Technical problems solved

In view of the deficiencies of the prior art, the present invention provides a high-efficiency screening device for secretin monoclonal antibodies, which can perform multiple screenings, has the advantages of high screening efficiency and thorough screening, and further solves the problems in the above-mentioned background technology.

(2) Technical solutions

In order to realize the above-mentioned advantages of multiple screening, high screening efficiency and thorough screening, the specific technical scheme adopted by the present utility model is as follows:

A high-efficiency screening device for secretin monoclonal antibodies, comprising a device casing, a first equipment area located at the upper part of the device casing, a master control box, a second equipment area located in the middle of the device casing, and a third equipment area located at the lower end of the device casing , the inside of the first equipment area is provided with a first-level screening cylinder, the first-level screening cylinder includes a steel pipe and a support net, the top of the steel pipe is provided with a support net, and the inside of the support net is provided with a filter membrane, so The inner hollow top of the steel pipe is provided with a discharge valve, the top of the support net is provided with a sealing cover, the top of the first-stage screening cylinder is provided with a linear actuator, and the driving end of the linear actuator extends into the sealing cover, The extension end of the linear drive is provided with a pressure plate, the top of the primary screening cylinder is also provided with a feed port, and the bottom end of the feed port is provided with a hose, which extends through the sealing cover To the pressure plate, the hose is provided with a feeding valve, the inner bottom end of the primary screening cylinder is provided with a drain pipe, and the lower end of the drain pipe is connected with a secondary screening cylinder, the two The primary screening cylinder is located inside the second equipment area, the bottom end of the steel pipe is provided with a primary collection cylinder, and the primary collection cylinder is located in the second equipment area, and the internal structure of the secondary screening cylinder is the same as that of the primary screening cylinder. The bottom end of the secondary screening cylinder is provided with a secondary collection cylinder, and the secondary collection cylinder is located in the third equipment area, and one end of the secondary screening cylinder is provided with a waste liquid discharge pipe. The third equipment area is also provided with a motor, and the output shaft end of the motor is connected with a rotating shaft, the outer surface of the rotating shaft located in the third equipment area is provided with a second transmission mechanism, and the second transmission mechanism is driven The end is connected to the bottom end of the secondary screening drum, the outer surface of the rotating shaft located in the second equipment area is provided with a first transmission mechanism, and the driven end of the first transmission mechanism is connected to the outside of the steel pipe of the primary screening drum surface.

Further, the lowered height of the pressure plate is equal to the height of the sealing cover, the pressure plate is fixedly connected with the bottom end of the hose, and the pressure plate is provided with a feeding hole at the bottom end of the hose.

Further, the bottom end of the steel pipe is movably connected with the primary collection cylinder through a bearing, and the primary collection cylinder is communicated with the primary screening cylinder through the steel pipe.

Further, both the second transmission mechanism and the first transmission mechanism include two sets of fixed sprocket transmission chains, one set of fixed sprockets is fixedly installed on the rotating shaft, and the other set of fixed sprockets is fixedly installed on the first-stage screening drum and the second set of fixed sprockets. The outer wall of the steel pipe at the bottom end of the stage screening cylinder, and the transmission chain is driven around two sets of fixed sprockets.

Further, the bottom ends of the primary collection cylinder and the secondary collection cylinder are provided with a suction pipe, and the suction pipe extends to the outside of the device casing and is connected with a collection trolley, and the collection trolley is provided with a collection bottle.

Further, an equipment door and an electric door are arranged on the outer surface of the front end of the device casing, and a display screen and an operation button electrically connected to the main control box are arranged on the electric door.

(3) Beneficial effects

Compared with the prior art, the utility model provides a high-efficiency screening device for secretin monoclonal antibodies, which has the following beneficial effects:

(1) The utility model can screen and classify different micron-level cell material sizes through the cooperation of the first-level screening cylinder and the second-level screening cylinder, and the screening is more efficient and thorough.

(2), the utility model cooperates with each other through the set linear driver and the pressure plate, when the primary screening cylinder and the secondary screening cylinder perform centrifugal motion, the pressure in the cylinder is increased, so that the mixed liquid quickly passes through the filter membrane, saving the screening time .

(3) The utility model adopts a set of motors to simultaneously drive the primary screening cylinder and the secondary screening cylinder for screening through the provided first transmission mechanism and the second transmission mechanism, which is compact in structure and saves energy consumption.

Description of drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the accompanying drawings required in the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only of the present invention. For some embodiments of the present invention, for those of ordinary skill in the art, other drawings can also be obtained from these drawings without any creative effort.

1 is a schematic diagram of the three-dimensional structure of a high-efficiency screening device for secretin monoclonal antibodies according to an embodiment of the present invention;

2 is a schematic diagram of the internal structure of a high-efficiency screening device for secretin monoclonal antibodies according to an embodiment of the present invention;

Fig. 3 is according to the first-level screening cylinder structural representation of Fig. 2;

FIG. 4 is a schematic diagram of a partial structure according to FIG. 3 .

In the picture:

1. Device shell; 2. The first equipment area; 3. The second equipment area; 4. The third equipment area; 5. The main control box; 603, sealing cover; 604, steel pipe; 605, drain pipe; 606, linear drive; 607, pressure plate; 608, hose; 609, feed valve; 610, support net; 611, discharge valve; 612, bearing ; 7. Primary collection cylinder; 8. Secondary screening cylinder; 9. Secondary collection cylinder; 10. Waste liquid discharge pipe; 11. Motor; 12. Second transmission mechanism; ; 15. Equipment door; 16. Electric door.

Detailed ways

In order to further illustrate each embodiment, the present invention provides accompanying drawings, which are part of the disclosure of the present invention, which are mainly used to illustrate the embodiments, and can be used to explain the operation principles of the embodiments in conjunction with the relevant descriptions in the specification, With reference to these contents, those of ordinary skill in the art will understand other possible implementations and advantages of the present invention. Components in the figures are not drawn to scale, and similar component symbols are generally used to denote similar components.

According to the embodiments of the present invention, a high-efficiency screening device for secretin monoclonal antibodies is provided.

The present utility model will now be further described with reference to the accompanying drawings and specific embodiments. As shown in Figs. 1-4, a high-efficiency screening device for secretin monoclonal antibodies according to an embodiment of the present utility model comprises a device casing 1, which is located in the device casing 1. 1. The first equipment area 2 in the upper part, the main control box 5 for controlling the automation of the device, the second equipment area 3 located in the middle of the device shell 1, and the third equipment area 4 located at the lower end of the device shell 1. The interior of the first equipment area 2 is set There is a primary screening cylinder 6. The primary screening cylinder 6 includes a steel pipe 604 and a support net 610. The top end of the steel pipe 604 is provided with a support net 610, and the inside of the support net 610 is provided with a filter membrane 602. The inner hollow top of the steel pipe 604 is provided with an outlet. The feeding valve 611 and the discharging valve 611 are solenoid valves, the top of the support net 610 is provided with a sealing cover 603, the top of the primary screening cylinder 6 is provided with a linear actuator 606, and the driving end of the linear actuator 606 extends into the sealing cover 603, The extension end of the linear drive 606 is provided with a pressure plate 607, the top of the primary screening cylinder 6 is also provided with a feed port 601, and the bottom end of the feed port 601 is provided with a hose 608, which extends through the sealing cover 603 To the pressure plate 607, the hose 608 is provided with a feed valve 609, the feed valve 609 is a solenoid valve, the inner bottom end of the primary screening cylinder 6 is provided with a drain pipe 605, and the lower end of the drain pipe 605 is connected with a The secondary screening cylinder 8, the secondary screening cylinder 8 is located inside the second equipment area 3, the bottom end of the steel pipe 604 is provided with a primary collecting cylinder 7, and the primary collecting cylinder 7 is located in the second equipment area 3, the secondary screening cylinder The internal structure of 8 is the same as that of the primary screening cylinder 6, the bottom end of the secondary screening cylinder 8 is provided with a secondary collecting cylinder 9, and the secondary collecting cylinder 9 is located in the third equipment area 4, and one end of the secondary screening cylinder 8 is provided with There is a waste liquid discharge pipe 10, the third equipment area 4 is also provided with a motor 11, and the output shaft end of the motor 11 is connected with a rotating shaft 13, and the outer surface of the rotating shaft 13 located in the third equipment area 4 is provided with a second transmission mechanism 12, And the driven end of the second conveying mechanism 12 is connected to the bottom end of the secondary screening cylinder 8, the outer surface of the rotating shaft 13 located in the second equipment area 3 is provided with a first conveying mechanism 14, and the driven end of the first conveying mechanism 14 is The ends are attached to the outer surface of the steel tube 604 of the primary screening cylinder 6 .

In one embodiment, the descending height of the pressure plate 607 is equal to the height of the sealing cover 603, the pressure plate 607 is fixedly connected with the bottom end of the hose 608, and the pressure plate 607 is located at the bottom end of the hose 608 with a feeding hole, It is convenient to put the mixture liquid into the first-stage screening drum 6 .

In one embodiment, the bottom end of the steel pipe 604 is movably connected with the primary collection cylinder 7 through the bearing 612, and the primary collection cylinder 7 is communicated with the primary screening cylinder 6 through the steel pipe 604, so that the screening material in the filter membrane 602 follows the The steel pipe 604 flows into the primary collection drum 7 .

In one embodiment, both the second transmission mechanism 12 and the first transmission mechanism 14 include two sets of fixed sprocket transmission chains, one set of fixed sprockets is fixedly installed on the rotating shaft 13, and the other set of fixed sprockets is fixedly installed on the first stage The outer wall of the steel pipe 604 at the bottom of the screening drum 6 and the secondary screening drum 8, the transmission chain is driven around two sets of fixed sprockets, so that the same motor 11 can drive the primary screening drum 6 and the secondary screening drum 8 at the same time, saving energy consumption.

In one embodiment, the bottom ends of the primary collection cylinder 7 and the secondary collection cylinder 9 are provided with suction pipes, and the suction pipes extend to the outside of the device casing 1 and are connected to a collection trolley (not shown in the figure), which collects There is a collection bottle on the trolley, which is convenient for centralized extraction of screening materials to the operating table.

In one embodiment, an equipment door 15 and an electrical door 16 are provided on the outer surface of the front end of the device casing 1 to facilitate opening for normal maintenance and repair of the interior, and the electrical door 16 is provided with a display that is electrically connected to the main control box 5 The screen and operation buttons are convenient for the operator to operate.

Working principle: The cell mixture liquid enters the hose 608 through the feeding port 601 at the top of the primary screening cylinder 6 and then enters the filter membrane 602 of the primary screening cylinder 6. After the feeding is completed, close the feeding valve on the hose 608 609, start the motor 11 to drive the rotating shaft 13 to drive the first transmission mechanism 14 and the steel pipe 604 of the primary screening cylinder 6 to rotate, so that the whole of the sealing cover 603, the support net 610 and the filter membrane 602 rotates. , the linear driver 606 of the primary screening cylinder 6 starts to drive the pressure plate 607 to press down, increasing the internal pressure of the filter membrane 602, so that the cell mixture liquid that is smaller than the particle size of the filter membrane 602 quickly passes through the filter membrane 602 and is larger than the filter membrane 602 particle size The cell mixture is collected by opening the discharge valve 611 and falling from the steel pipe 604 to the primary collection cylinder 7. After the primary screening cylinder 6 is screened, the feed valve 609 on the hose 608 of the secondary screening cylinder 8 is opened, and the mixture liquid Put it into the secondary screening cylinder 8 and continue to repeat the above-mentioned actions. The second transmission mechanism 12 drives the steel pipe 604 of the secondary screening cylinder 8 to rotate. The liquid molecules of particle size are discharged through the waste liquid discharge pipe 10, and other mixtures flow into the secondary collection cylinder 9 for collection.

In the present utility model, unless otherwise expressly specified and limited, terms such as "installation", "arrangement", "connection", "fixation", "swivel connection" should be understood in a broad sense, for example, it may be a fixed connection, It can also be detachable connection or integrated; it can be mechanical connection or electrical connection; it can be directly connected or indirectly connected through an intermediate medium, it can be the internal communication of two elements or the interaction of two elements Unless otherwise clearly defined, those of ordinary skill in the art can understand the specific meanings of the above terms in the present invention according to specific situations.

The above are only preferred embodiments of the present invention, and are not intended to limit the present invention. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the within the scope of protection of the present invention.

Claims (6)

1. The high-efficiency screening device for the monoclonal antibody of the Gastrin is characterized by comprising a device shell (1), a first equipment area (2) positioned at the upper part of the device shell (1), a master control box (5), a second equipment area (3) positioned in the middle of the device shell (1) and a third equipment area (4) positioned at the lower end of the device shell (1), wherein a first-stage screening cylinder (6) is arranged inside the first equipment area (2), the first-stage screening cylinder (6) comprises a steel pipe (604) and a supporting net (610), the top end of the steel pipe (604) is provided with the supporting net (610), a filter membrane (602) is arranged inside the supporting net (610), the hollow top end inside the steel pipe (604) is provided with a discharge valve (611), the top end of the supporting net (610) is provided with a sealing cover (603), the top end of the first-stage screening cylinder (6) is provided with a linear driver (606), the driving end of the linear driver (606) extends into the sealing cover (603), the extending end of the linear driver (606) is provided with a pressure plate (607), the top end of the primary screening cylinder (6) is also provided with a feeding hole (601), the bottom end of the feeding hole (601) is provided with a hose (608), the hose (608) penetrates through the sealing cover (603) and extends onto the pressure plate (607), the hose (608) is provided with a feeding valve (609), the bottom end inside the primary screening cylinder (6) is provided with a liquid discharge pipe (605), the lower end of the liquid discharge pipe (605) is connected with a secondary screening cylinder (8), the secondary screening cylinder (8) is positioned inside the second equipment area (3), the bottom end of the steel pipe (604) is provided with a primary collecting cylinder (7), and the primary collecting cylinder (7) is positioned in the second equipment area (3), the internal structure of the secondary screening cylinder (8) is the same as that of the primary screening cylinder (6), a secondary collecting cylinder (9) is arranged at the bottom end of the secondary screening cylinder (8), the secondary collecting cylinder (9) is positioned in a third equipment area (4), one end of the secondary screening cylinder (8) is provided with a waste liquid discharge pipe (10), the third equipment area (4) is also provided with a motor (11), and the output shaft end of the motor (11) is connected with a rotating shaft (13), the outer surface of the rotating shaft (13) positioned in the third equipment area (4) is provided with a second transmission mechanism (12), and the driven end of the second transmission mechanism (12) is connected with the bottom end of the secondary screening cylinder (8), a first transmission mechanism (14) is arranged on the outer surface of the rotating shaft (13) which is positioned in the second equipment area (3), and the driven end of the first transmission mechanism (14) is connected to the outer surface of the steel pipe (604) of the primary screening cylinder (6).

2. The apparatus for the high-efficiency screening of the monoclonal antibody against secretin according to claim 1, wherein the pressure plate (607) is lowered to a height equal to that of the sealing cap (603), the pressure plate (607) is fixedly connected to the bottom end of the hose (608), and the pressure plate (607) is provided with a blanking hole at the bottom end of the hose (608).

3. The device for screening monoclonal antibodies against secretin according to claim 2, wherein the bottom end of the steel tube (604) is movably connected to the primary collection cylinder (7) through a bearing (612), and the primary collection cylinder (7) is communicated with the primary screening cylinder (6) through the steel tube (604).

4. The apparatus for the high-efficiency screening of the monoclonal antibody against secretin according to claim 1, wherein the second conveyor mechanism (12) and the first conveyor mechanism (14) each comprise two sets of fixed sprocket conveyor chains, one set of fixed sprocket conveyor chains is fixedly mounted on the rotating shaft (13), the other set of fixed sprocket conveyor chains is fixedly mounted on the outer walls of the steel pipes (604) at the bottom ends of the primary screening drum (6) and the secondary screening drum (8), and the conveyor chains are driven around the two sets of fixed sprocket conveyor chains.

5. The device for screening monoclonal antibodies against secretin according to claim 1, wherein the primary collection canister (7) and the secondary collection canister (9) are provided with pumping tubes at their bottom ends, and the pumping tubes extend out of the device housing (1) and are connected to a collection cart on which collection bottles are disposed.

6. The device for screening monoclonal antibodies against secretin according to claim 1, wherein the device housing (1) is provided with an equipment door (15) and an electrical door (16) on the front outer surface, and the electrical door (16) is provided with a display screen and an operation button electrically connected to the master control box (5).

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